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Zsuzsa Sárkány. Completed the Mestrado integrado in Biology - Chemistry in 1996/07/01 by Eötvös Loránd University, Faculty of Science, Budapest, Hungary and Doutoramento in Structural Biochemistry in 2006/06/22 by Eötvös Loránd University, Faculty of Science, Department of Biochemistry, Budapest, Hungary. Is Contracted Researcher in Universidade do Porto Instituto de Investigação e Inovação em Saúde. Published 16 articles in journals. Supervised 1 MSc dissertation(s) e co-supervised 1. Participates and/or participated as PhD Student Fellow in 1 project(s), Post-doc Fellow in 5 project(s), Research Fellow in 1 project(s) and Researcher in 2 project(s). Works in the area(s) of Natural sciences with emphasis on Biological Sciences with emphasis on Biophysics, Natural sciences with emphasis on Biological Sciences with emphasis on Biochemical Research Methods and Natural sciences with emphasis on Biological Sciences with emphasis on Molecular Biology. In his curriculum Ciência Vitae the most frequent terms in the context of scientific, technological and artistic-cultural output are: Project funding; Fe-S cluster biogenesis; Oxidative stress; Transcription factor; Three-dimensional structure; Parkinson's Disease; Machado-Joseph Disease; Toxic Oligomers; Reposicionamento de Fármacos; Medical and health sciences; Colloid aggregation; Biological condensates; Liquid-liquid phase separation; Chemical kinetics; Exact sciences; Engineering and technology; Engineering and technology/Chemical engineering; Candida; genetic code; cell wall; kinases; Engineering and technology/Industrial biotechnology; Natural sciences; Natural sciences/Biological sciences; .
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Identification

Personal identification

Full name
Zsuzsa Sárkány
Gender
Female

Citation names

  • Sárkány, Zsuzsa

Author identifiers

Ciência ID
5E1C-24E7-A8E8
ORCID iD
0000-0001-9185-8210

Email addresses

  • zsarkany@i3s.up.pt (Professional)
  • zs.sarkany@gmail.com (Personal)

Telephones

Mobile phone
  • 926907489 (Personal)
Telephone
  • 220408800 Ext.: 6160 (Professional)

Addresses

  • Rua Alfredo Allen 208, Porto, Portugal, 4200-135 Porto, 4200-135, Porto, Porto, Portugal (Professional)
  • RUA DOS BRASILEIROS, 94, 4455-071, LAVRA, Lavra-Matosinhos, Portugal (Personal)

Knowledge fields

  • Natural sciences - Biological Sciences - Biophysics
  • Natural sciences - Biological Sciences - Biochemical Research Methods
  • Natural sciences - Biological Sciences - Molecular Biology

Languages

Language Speaking Reading Writing Listening Peer-review
Hungarian (Mother tongue)
Portuguese Upper intermediate (B2) Upper intermediate (B2) Upper intermediate (B2) Upper intermediate (B2) Upper intermediate (B2)
English Upper intermediate (B2) Upper intermediate (B2) Upper intermediate (B2) Upper intermediate (B2) Upper intermediate (B2)
Education
Degree Classification
2006/06/22
Concluded
 Structural Biochemistry (Doutoramento)
Eötvös Loránd University, Faculty of Science, Department of Biochemistry, Budapest, Hungary, Hungary
"THE CATALYTIC MECHANISM OF RHINOVIRUS 2A AND POLIOVIRUS 3C PROTEASES" (THESIS/DISSERTATION)
 Summa cum laude
1999/09/07 - 2003/06/21
Concluded
 Structural Biochemistry (Curso de doutoramento (conclusão de unidades curriculares))
Eötvös Loránd University, Faculty of Science, Department of Biochemistry, Hungary
 Summa cum laude
1990/09/03 - 1996/07/01
Concluded
 Biology - Chemistry (Mestrado integrado)
Eötvös Loránd University, Faculty of Science, Budapest, Hungary, Hungary
"Investigation of the Unionid population from the river White Cris" (THESIS/DISSERTATION)
Affiliation

Science

Category
Host institution 		Employer
2019/04/01 - Current Contracted Researcher (Research) Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2018/04/01 - 2019/03/31 Postdoc (Research) Faculdade de Engenharia da Universidade do Porto, Departamento de Engenharia Química, Portugal
2017/05/01 - 2018/01 Contracted Researcher (Research) Universidade do Porto Instituto de Investigação e Inovação em Saúde, Biochemical and Biophysical Technologies Platform, Portugal
2016/05/01 - 2017/04/30 Postdoc (Research) Universidade do Porto Instituto de Investigação e Inovação em Saúde, Biomolecular Structure and Function Group, Portugal
2011/05/01 - 2016/04/30 Postdoc (Research) Universidade do Porto Instituto de Biologia Molecular e Celular, Protein Crystallography Group, Portugal
2007/05/01 - 2011/04/30 Postdoc (Research) Universidade do Porto Instituto de Biologia Molecular e Celular, Molecular Structure Group, Portugal
1997/08/01 - 2003/04/30 Researcher (Research) Institute of Enzymology of the Hungarian Academy of Sciences, Budapest, Hungary, Hungary

Others

Category
Host institution 		Employer
2003/05/01 - 2007/04/30 Living in Portugal for family reasons, bringing up two sons born here. In the meantime, Ph.D. exams and thesis defense completed back in Budapest, Hungary. Institute of Enzymology of the Hungarian Academy of Sciences, Budapest, Hungary, Hungary
1996/08/01 - 1997/07/31 Biology and chemistry teacher in high school Verebély László High School, Budapest, Hungary, Hungary
Projects

Grant

Designation Funders
2022/01/01 - Current Colloid aggregation activity of intrinsically disordered polypeptides that phase separate into neurotoxic condensates
PTDC/QUI-COL/2444/2021
Researcher
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
 Fundação para a Ciência e a Tecnologia
2019/01/01 - 2022/02 Targeting diffusible oligomers of alpha-synuclein and ataxin-3: a drug repurposing opportunity for the treatment of neurodegenerative diseases
PTDC/MED-FAR/31323/2017
Researcher
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2018/03 - 2019/03 Soluble oligomers of alpha-synuclein studied as possible drug targets for the treatment of Parkinson`s disease
FEUP-LEPABE-SINUCLEÍNA/NORTE-01-0145-FEDER-000005
Post-doc Fellow
Fundação para a Ciência e a Tecnologia
Concluded
2016/05/01 - 2017/04/30 Novel therapeutic tools for Machado-Joseph Disease: Lessons from molecular interactions
NORTE-01-0145-FEDER-000008
Post-doc Fellow
Fundação para a Ciência e a Tecnologia
2013/07/01 - 2015/06/30 Biochemical and structural characterization of gene regulation by IscR, the Fe-S cluster biogenesis pathway regulator
PTDC/BBB-BEP/2127/2012
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2012 - 2015 Ambiguity and virulence in a human pathogen
PTDC/BBB-BEP/0695/2012
Post-doc Fellow
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
 Fundação para a Ciência e a Tecnologia
2007 - 2009 Plant TTL and Human RAGE: Any common features between the signaling pathways in plant development and human nerve regeneration?
PTDC/SAU-NEU/69123/2006
Post-doc Fellow
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
 Fundação para a Ciência e a Tecnologia
2002/01/01 - 2003 Structural basis of oligopeptidase regulation and catalysis: acylaminoacyl peptidase.
066099
PhD Student Fellow
Institute of Enzymology of the Hungarian Academy of Sciences, Budapest, Hungary, Hungary
 Wellcome Trust
Concluded
1998 - 1999 The mechanism of action of cysteine proteases: poliovirus 3C and rhinovirus 2A”
Hungarian National Research Fund Grant - OTKA T/9 T022808
Research Fellow
Institute of Enzymology of the Hungarian Academy of Sciences, Budapest, Hungary, Hungary
Concluded

Contract

Designation Funders
2010/05/01 - 2016/04 STRUCTURAL IMPACT OF CUG AMBIGUITY: STRUCTURAL AND FUNCTIONAL CHARACTERIZATION OF PROTEINS INVOLVED IN THE SIGNAL TRANSDUCTION PATHWAY IN CANDIDA ALBICANS
SFRH/BPD/63465/2009
Post-doc Fellow
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
 Fundação para a Ciência e a Tecnologia
Outputs

Publications

Conference abstract
  1. Saavedra, Joana; Sárkány, Zsuzsa; Cardoso, Isabel; Martins, Pedro. "Preclinical development of small-molecule inhibitors of non-amyloidogenic aggregation of alpha-synuclein in Parkinson's Disease". Paper presented in International Conference on Alzheimer's and Parkinson's Diseases and related neurological disorders (AD/PD 2022), Barcelona, 2022.
    Published
Conference poster
  1. Podlasiak, Martyna; Manso, J.A.; Macedo-Ribeiro, Sandra; Sárkány, Zsuzsa; Barbosa Pereira, Pedro José. "Unravelling Molecular Insights into Machado-Joseph Disease through Ataxin-3 Interactions with Nanobodies". Paper presented in 10th McBiology Symposium "A Decade Building Science", 2024.
  2. Sárkány, Zsuzsa; Figueiredo, Francisco; Silva, Alexandra; Vilasboas-Campos, Daniela; Maciel, Patrícia; Teixeira-Castro, Andreia; MARTINS, PEDRO MIGUEL; Macedo-Ribeiro, Sandra. "Repurposing dopaminergic drugs to prevent Ataxin 3 aggregation". Paper presented in III PhasAGE International Conference:Multiscale understanding of protein aggregation and biomolecular condensates in aging and disease, 2023.
  3. Podlasiak, Martyna; Silva, Alexandra; Sárkány, Zsuzsa; Morais-de-Sá, Eurico; Barbosa Pereira, Pedro J.; Macedo-Ribeiro, Sandra. "Ataxin-3-targeting nanobodies in Machado-Joseph Disease". Paper presented in III PhasAGE International Conference: "A multiscale understanding of protein aggregation and phase separation: from molecules to the brain", 2023.
  4. Podlasiak, Martyna; Sárkány, Zsuzsa; Figueiredo, Francisco; Macedo-Ribeiro, Sandra; Barbosa Pereira, Pedro J.; Silva, Alexandra. "Development of a nanobody-based toolbox for Machado-Joseph Disease". Paper presented in II PhasAGE-VIB International Conference: Biomolecular Condensates in Biology and Disease, 2022.
  5. Saavedra, Joana; Sárkány, Zsuzsa; Cardoso, Isabel; Martins, Pedro. "Application of repurposed compounds as inhibitors of the non-amyloidogenic oligomerization of alpha-synuclein in Parkinson's Disease". Paper presented in 8th edition of the Molecular and Cell Biology Symposium - Let's have a moment of science!¿, 2022.
  6. Saavedra, Joana; Sárkány, Zsuzsa; Cardoso, Isabel; Martins, Pedro. "Drug repurposing compounds rescuing cell toxicity induced by soluble oligomers of alpha-synuclein". Paper presented in XVII Meeting of the Portuguese Society for Neuroscience (SPN 2021), 2021.
  7. Sárkány, Z. (5E1C-24E7-A8E8); Martins P.; Rocha, F.; Damas, A.M.; Macedo-Ribeiro, S.. "High-Throughput screening of protein aggregation modulators using chemical kinetic strategies". Paper presented in 14th International Conference on Alzheimer's and Parkinson's Diseases, Lisbon, Portugal, 2019.
  8. Gomes, M.N.; A., Silva; Manso, J.; Almeida, A.; Sárkány, Z. (5E1C-24E7-A8E8); Pereira, P.J.B.. "Crystal structure of an Ataxin-3 binding nanobody". Paper presented in 4th Symposium on Non-Globular Proteins (NGP-NET), Druskininkai, Lithuania, 2018.
  9. A., Silva; Almeida, A.; Sárkány, Z. (5E1C-24E7-A8E8); Macedo-Ribeiro, S.. "Molecular tools to tackle protein aggregation in Machado-Joseph disease". Paper presented in 3rd Symposium on Non-Globular Proteins (NGP-NET), Kosice, Slovakia, 2017.
  10. Fraga, J.; Teixeira, I.; Sárkány, Z.(5E1C-24E7-A8E8); Silva, A.,; Pereira, P.J.B.; Macedo-Ribeiro, S.. "Molecular sensors of codon ambiguity in C. albicans signaling pathways". Paper presented in Instruct Biennial Structural Biology Meeting Florence, Italy, 2015.
  11. A., Silva; Sárkány, Z. (5E1C-24E7-A8E8); Carvalho, V.; Moreira, M.; Santos, M.S.; Pereira, P.J. B.; Macedo-Ribeiro, S.. "Is there a link between genetic code ambiguity and virulence in the human pathogen, Candida albicans?". Paper presented in 5th FEBS Advanced Lecture Course on Human Fungal Pathogens, La Colle-sur-Loup, Nice, France, 2013.
  12. Sárkány, Zsuzsa; Carvalho, V.; Pereira, P. J.; Macedo-Ribeiro, S.. "CUG ambiguity in the structure of Ras1 and Cek1 from Candida albicans". Paper presented in 22nd IUBMB & 37th FEBS Congress, Sevilla, Spain, 2012.
  13. Sárkány, Z. (5E1C-24E7-A8E8); Svergun, D.; Ikonen, T.; Ferreira-da-Silva, F.; Saraiva, M. J.; Damas, A. M.. "Solution structure and self assembly properties of soluble RAGE". Paper presented in 8 th European Biophysics Congress, Budapest, Hungary, 2011.
  14. Sárkány, Z. (5E1C-24E7-A8E8); Pessoa, J.; Ferreira-da-Silva, F.,; Jianming Li. "TTL- a bifunctional enzyme in the ureide pathway". Paper presented in 35th FEBS Congress, Gothenburg, Sweden, 2010.
  15. Sárkány, Z. (5E1C-24E7-A8E8); Svergun, D.; Ikonen, T.; Ferreira-da-Silva, F.; Saraiva, M.J.; Damas, A.M. "Solution structure of the extracellular region of the Receptor for Advanced Glycation End products". Paper presented in XVII National Congress of Biochemistry, Porto, Portugal, 2010.
  16. Pessoa, J.; Sárkány, Z. (5E1C-24E7-A8E8); Ferreira-da-Silva, F.; Damas, A. M.. "Structural and functional studies on Arabidopsis thaliana Transthyretin-like protein". Paper presented in XVI National Congress of Biochemistry, Ponta Delgada,Portugal, 2008.
  17. Sárkány, Z. (5E1C-24E7-A8E8); Ferreira-da-Silva, F.; Almeida, M.; Damas, A. M.. "Structural studies on the extracellular region of the Receptor for Advanced Glycation End products". Paper presented in 1st Meeting on Medicinal Chemistry, Porto, Portugal, 2008.
Journal article
  1. Zsuzsa Sárkány; Francisco Figueiredo; Sandra Macedo-Ribeiro; Pedro M. Martins. "NAGPKin: Nucleation-and-growth parameters from the kinetics of protein phase separation". Molecular Biology of the Cell (2023): http://dx.doi.org/10.1091/mbc.e23-07-0289.
    10.1091/mbc.e23-07-0289
  2. Francisco Figueiredo; Zsuzsa Sárkány; Alexandra Silva; Daniela Vilasboas-Campos; Patrícia Maciel; Andreia Teixeira-Castro; Pedro M. Martins; Sandra Macedo-Ribeiro. "Drug repurposing of dopaminergic drugs to inhibit ataxin-3 aggregation". Biomedicine & Pharmacotherapy (2023): https://doi.org/10.1016/j.biopha.2023.115258.
    10.1016/j.biopha.2023.115258
  3. José A. Manso; Arturo Carabias; Zsuzsa Sárkány; José M. de Pereda; Pedro José Barbosa Pereira; Sandra Macedo-Ribeiro; Wayne A. Hendrickson. "Pathogen-specific structural features of Candida albicans Ras1 activation complex: uncovering new antifungal drug targets". mBio (2023): https://doi.org/10.1128/mbio.00638-23.
    10.1128/mbio.00638-23
  4. Sárkány, Zsuzsa; Rocha, Fernando; Bratek-Skicki, Anna; Tompa, Peter; Macedo-Ribeiro, Sandra; Martins, Pedro M.. "Quantification of Surface Tension Effects and Nucleation-and-Growth Rates during Self-Assembly of Biological Condensates". Advanced Science (2023): http://dx.doi.org/10.1002/advs.202301501.
    Published • 10.1002/advs.202301501
  5. Martins, Pedro M.; Navarro, Susanna; Silva, Alexandra; Pinto, Maria F.; Sárkány, Zsuzsa; Figueiredo, Francisco; Pereira, Pedro José Barbosa; et al. "MIRRAGGE – Minimum Information Required for Reproducible AGGregation Experiments". Frontiers in Molecular Neuroscience 13 (2020): http://dx.doi.org/10.3389/fnmol.2020.582488.
    10.3389/fnmol.2020.582488
  6. Fraga, Joana S.; Sárkány, Zsuzsa; Silva, Alexandra; Correia, Inês; Pereira, Pedro José Barbosa; Macedo-Ribeiro, Sandra. "Genetic code ambiguity modulates the activity of a C. albicans MAP kinase linked to cell wall remodeling". Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics 1867 6 (2019): 654-661. http://dx.doi.org/10.1016/j.bbapap.2019.02.004.
    Published • 10.1016/j.bbapap.2019.02.004
  7. Sárkány, Zsuzsa; Rocha, Fernando; Damas, Ana M.; Macedo-Ribeiro, Sandra; Martins, Pedro M.. "Chemical Kinetic Strategies for High-Throughput Screening of Protein Aggregation Modulators". Chemistry - An Asian Journal 14 4 (2019): 500-508. http://dx.doi.org/10.1002/asia.201801703.
    10.1002/asia.201801703
  8. Gomes, João R.; Sárkány, Zsuzsa; Teixeira, Anabela; Nogueira, Renata; Cabrito, Inês; Soares, Hugo; Wittelsberger, Angela; et al. "Anti-TTR Nanobodies Allow the Identification of TTR Neuritogenic Epitope Associated with TTR-Megalin Neurotrophic Activities". ACS Chemical Neuroscience 10 1 (2018): 704-715. http://dx.doi.org/10.1021/acschemneuro.8b00502.
    10.1021/acschemneuro.8b00502
  9. Alexandra Silva; Zsuzsa Sarkany; Joana Fraga; Pablo Taboada; Sandra Macedo-Ribeiro; Pedro Miguel Martins. "Probing the Occurrence of Soluble Oligomers through Amyloid Aggregation Scaling Laws". Biomolecules (2018): http://www.mdpi.com/2218-273X/8/4/108.
    10.3390/biom8040108
  10. Sárkány Z; Silva A; Pereira PJ; Macedo-Ribeiro S. "Ser or Leu: structural snapshots of mistranslation in Candida albicans.". (2014): http://europepmc.org/abstract/med/25988168.
    10.3389/fmolb.2014.00027
  11. Sárkány, Z.; Ikonen, T.P.; Ferreira-da-Silva, F.; Saraiva, M.J.; Svergun, D.; Damas, A.M.. "Solution structure of the soluble receptor for advanced glycation end products (sRAGE)". Journal of Biological Chemistry 286 43 (2011): 37525-37534. http://www.scopus.com/inward/record.url?eid=2-s2.0-80054814015&partnerID=MN8TOARS.
    10.1074/jbc.M111.223438
  12. Pessoa, J.; Sárkány, Z.; Ferreira-da-Silva, F.; Martins, S.; Almeida, M.R.; Li, J.; Damas, A.M.. "Functional characterization of Arabidopsis thaliana transthyretin-like protein". BMC Plant Biology 10 (2010): http://www.scopus.com/inward/record.url?eid=2-s2.0-77949472438&partnerID=MN8TOARS.
    10.1186/1471-2229-10-30
  13. Martins, P.M.; Pessoa, J.; Sàrkàny, Z.; Rocha, F.; Damas, A.M.. "Rationalizing protein crystallization screenings through water equilibration theory and protein solubility data". Crystal Growth and Design 8 12 (2008): 4233-4243. http://www.scopus.com/inward/record.url?eid=2-s2.0-61549122663&partnerID=MN8TOARS.
    10.1021/cg8006958
  14. Sárkány, Z.; Polgár, L.. "The unusual catalytic triad of poliovirus protease 3C". Biochemistry 42 2 (2003): 516-522. http://www.scopus.com/inward/record.url?eid=2-s2.0-0037457917&partnerID=MN8TOARS.
    10.1021/bi027004w
  15. Sárkány, Z.; Szeltner, Z.; Polgár, L.. "Thiolate-imidazolium ion pair is not an obligatory catalytic entity of cysteine peptidases: The active site of picornain 3C". Biochemistry 40 35 (2001): 10601-10606. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035807060&partnerID=MN8TOARS.
    10.1021/bi010550p
  16. Sárkány, Z.; Skern, T.; Polgár, L.. "Characterization of the active site thiol group of rhinovirus 2A proteinase". FEBS Letters 481 3 (2000): 289-292. http://www.scopus.com/inward/record.url?eid=2-s2.0-0342409311&partnerID=MN8TOARS.
    10.1016/S0014-5793(00)02024-X
Preprint
  1. Zsuzsa Sárkány; Tiago Gião; Márcia Almeida Liz; Antoni Planas; Sandra Macedo-Ribeiro; Isabel Cardoso; Gemma Arsequell; Pedro M. Martins. "Transthyretin has conformation-selective proteolytic activity against a-synuclein". 2023. https://doi.org/10.1101/2023.08.10.552896.
    10.1101/2023.08.10.552896

Intellectual property

Provisional application for patent
  1. 2024. "Compounds for use in the treatment of Machado-Joseph Disease through binding at a 'Dopamine docking site' in Ataxin 3.".
  2. 2020. "TREATMENT FOR SYNUCLEINOPATHIES USING INHIBITORS OF NON-AMYLOIDOGENIC ALPHA-SYNUCLEIN AGGREGATION".
Activities

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2022/02 - 2022/07/21 Uncovering the Liquid-Liquid Phase Separation Behavior of Ataxin-3
Supervisor
 Bioengenharia (Master)
Universidade do Porto Faculdade de Engenharia, Portugal
2011 - 2012/07/19 "Cek1 e Ras1 de C. albicans: clonagem, expressão e purificação" "C. albicans Cek1 and Ras1: cloning, expression and purification"
Co-supervisor of Vanessa Isabel Dias Ribeiro de Carvalho
Biologia Aplicada (Master)
Universidade de Aveiro Departamento de Biologia, Portugal

Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal