???global.info.a_carregar???
Identification

Personal identification

Full name
Alfredo Castello Palomares

Author identifiers

Ciência ID
601C-94C1-C332
ORCID iD
0000-0002-1499-4662
Education
Degree Classification
2003/07/01 - 2008/04/16
Concluded
 PhD in Molecular Biology (Postgraduate Certificate)
Universidad Autónoma de Madrid, Spain
"Alteraciones de la Expresión Génica Inducidas por Proteasas Virales" (THESIS/DISSERTATION)
1998/09/01 - 2003/06/30
Concluded
 Alfredo Castello Palomares (Bachelor)
Major in Biological Sciences
Universidad Autónoma de Madrid, Spain
Affiliation

Science

Category
Host institution 		Employer
2014/10/31 - 2021/11/30 Principal Investigator (Research) University of Oxford Department of Biochemistry, United Kingdom
2012/09/01 - 2014/10/30 Coordinating Researcher (Research) European Molecular Biology Laboratory, Germany
2009/07/01 - 2012/08/31 Postdoc (Research) European Molecular Biology Laboratory, Germany
2008/04/17 - 2009/06/30 Postdoc (Research) Universidad Autonoma de Madrid Centro de Biología Molecular Severo Ochoa, Spain
Projects

Grant

Designation Funders
2019/11 - 2021/11 Proteome-wide identification of RNA-binding proteins playing critical roles in virus infection
MR/R021562/1
 Medical Research Council
2014/10 - 2019/10 Global Approaches to Elucidate the Function of Post-transcriptional Networks in HIV-1 Infection
MR/L019434/1
 Medical Research Council
2016/05 - 2017/05 Comprehensive identification of host factors involved in the early steps of HIV infection
151/055
 University of Oxford
Outputs

Publications

Book
  1. Castello, A.; Horos, R.; Strein, C.; Fischer, B.; Eichelbaum, K.; Steinmetz, L.M.; Krijgsveld, J.; Hentze, M.W.. Comprehensive identification of RNA-binding proteins by RNA interactome capture. 2015.
    10.1007/978-1-4939-3067-8_8
Journal article
  1. Garcia-Moreno M; Noerenberg M; Ni S; Järvelin AI; González-Almela E; Lenz CE; Bach-Pages M; et al. "System-wide Profiling of RNA-Binding Proteins Uncovers Key Regulators of Virus Infection.". Molecular cell (2019): http://europepmc.org/abstract/med/30799147.
    10.1016/j.molcel.2019.01.017
  2. Horos, R.; Büscher, M.; Kleinendorst, R.; Alleaume, A.-M.; Tarafder, A.K.; Schwarzl, T.; Dziuba, D.; et al. "The Small Non-coding Vault RNA1-1 Acts as a Riboregulator of Autophagy". Cell 176 5 (2019): 1054-1067.e12. http://www.scopus.com/inward/record.url?eid=2-s2.0-85061744258&partnerID=MN8TOARS.
    10.1016/j.cell.2019.01.030
  3. Avolio R; Järvelin AI; Mohammed S; Agliarulo I; Condelli V; Zoppoli P; Calice G; et al. "Protein Syndesmos is a novel RNA-binding protein that regulates primary cilia formation.". Nucleic acids research (2018): http://europepmc.org/abstract/med/30260431.
    10.1093/nar/gky873
  4. Garcia-Moreno M; Järvelin AI; Castello A. "Unconventional RNA-binding proteins step into the virus-host battlefront.". Wiley interdisciplinary reviews. RNA (2018): http://europepmc.org/abstract/med/30091184.
    10.1002/wrna.1498
  5. Palomares, Alfredo. "Understanding RNP remodelling uncovers RBPs functionally required for viral replication". BioRxiv (2018):
    10.1101/350686
  6. Hobor F; Dallmann A; Ball NJ; Cicchini C; Battistelli C; Ogrodowicz RW; Christodoulou E; et al. "A cryptic RNA-binding domain mediates Syncrip recognition and exosomal partitioning of miRNA targets.". Nature communications (2018): http://europepmc.org/abstract/med/29483512.
    10.1038/s41467-018-03182-3
  7. Hentze MW; Castello A; Schwarzl T; Preiss T. "A brave new world of RNA-binding proteins.". Nature reviews. Molecular cell biology (2018): http://europepmc.org/abstract/med/29339797.
    10.1038/nrm.2017.130
  8. Palomares, Alfredo. "Identification of RNA-binding domains of RNA-binding proteins in cultured cells on a system-wide scale with RBDmap.". Nature protocols (2017): http://europepmc.org/abstract/med/29095441.
    10.1038/nprot.2017.106
  9. Moore S; Järvelin AI; Davis I; Bond GL; Castello A. "Expanding horizons: new roles for non-canonical RNA-binding proteins in cancer.". Current opinion in genetics & development (2017): http://europepmc.org/abstract/med/29216518.
    10.1016/j.gde.2017.11.006
  10. Pintacuda G; Wei G; Roustan C; Kirmizitas BA; Solcan N; Cerase A; Castello A; et al. "hnRNPK Recruits PCGF3/5-PRC1 to the Xist RNA B-Repeat to Establish Polycomb-Mediated Chromosomal Silencing.". Molecular cell (2017): http://europepmc.org/abstract/med/29220657.
    10.1016/j.molcel.2017.11.013
  11. Palomares, Alfredo. "RNA-binding activity of TRIM25 is mediated by its PRY/SPRY domain and is required for ubiquitination.". BMC biology (2017): http://europepmc.org/abstract/med/29117863.
    10.1186/s12915-017-0444-9
  12. Palomares, Alfredo. "Characterization of the African swine fever virus decapping enzyme during infection.". Journal of virology (2017): http://europepmc.org/abstract/med/29021398.
    10.1128/jvi.00990-17
  13. Palomares, Alfredo. "The small non-coding vault RNA1-1 acts as a riboregulator of autophagy". BioRxiv (2017):
    10.1101/177949
  14. Palomares, Alfredo. "Specific RNP capture with antisense LNA/DNA mixmers". RNA (2017):
    10.1261/rna.060798.117
  15. Palomares, Alfredo. "Plant RNA Interactome Capture: Revealing the Plant RBPome.". Trends Plant Sci (2017): http://europepmc.org/abstract/med/28478905.
    10.1016/j.tplants.2017.04.006
  16. Quintas, A.; Pérez-Núñez, D.; Sánchez, E.G.; Nogal, M.L.; Hentze, M.W.; Castelló, A.; Revilla, Y.. "Characterization of the African swine fever virus decapping enzyme during infection". Journal of Virology 91 24 (2017): http://www.scopus.com/inward/record.url?eid=2-s2.0-85035779251&partnerID=MN8TOARS.
    10.1128/JVI.00990-17
  17. Vasiliy O. Sysoev; Bernd Fischer; Christian K. Frese; Ishaan Gupta; Jeroen Krijgsveld; Matthias W. Hentze; Alfredo Castello; Anne Ephrussi. "Global changes of the RNA-bound proteome during the maternal-to-zygotic transition in Drosophila". Nature Communications 7 1 (2016): https://doi.org/10.1038/ncomms12128.
    10.1038/ncomms12128
  18. Palomares, Alfredo. "The Cardiomyocyte RNA-Binding Proteome: Links to Intermediary Metabolism and Heart Disease.". Cell Reports (2016): http://europepmc.org/abstract/med/27452465.
  19. Castello, Alfredo. "Comprehensive Identification of RNA-Binding Domains in Human Cells.". Mol Cell (2016): http://europepmc.org/abstract/med/27453046.
  20. Liepelt A; Naarmann-de Vries IS; Simons N; Eichelbaum K; Foehr S; Archer SK; Castello A; et al. "Identification of RNA-binding proteins in macrophages by interactome capture.". (2016): http://europepmc.org/abstract/med/27281784.
  21. Castello, Alfredo. "The expanding universe of ribonucleoproteins: of novel RNA-binding proteins and unconventional interactions.". (2016): http://link.springer.com/article/10.1007%2Fs00424-016-1819-4.
  22. Castello, Alfredo. "The new (dis)order in RNA regulation.". (2016):
    10.1186/s12964-016-0132-3
  23. Castello, A.; Fischer, B.; Frese, C.K.; Horos, R.; Alleaume, A.-M.; Foehr, S.; Curk, T.; Krijgsveld, J.; Hentze, M.W.. "Comprehensive Identification of RNA-Binding Domains in Human Cells". Molecular Cell 63 4 (2016): 696-710. http://www.scopus.com/inward/record.url?eid=2-s2.0-84992740396&partnerID=MN8TOARS.
    10.1016/j.molcel.2016.06.029
  24. Beckmann, B.M.; Castello, A.; Medenbach, J.. "The expanding universe of ribonucleoproteins: of novel RNA-binding proteins and unconventional interactions". Pflugers Archiv European Journal of Physiology 468 6 (2016): 1029-1040. http://www.scopus.com/inward/record.url?eid=2-s2.0-84966570560&partnerID=MN8TOARS.
    10.1007/s00424-016-1819-4
  25. Liao, Y.; Castello, A.; Fischer, B.; Leicht, S.; Föehr, S.; Frese, C.K.; Ragan, C.; et al. "The Cardiomyocyte RNA-Binding Proteome: Links to Intermediary Metabolism and Heart Disease". Cell Reports 16 5 (2016): 1456-1469. http://www.scopus.com/inward/record.url?eid=2-s2.0-84989890443&partnerID=MN8TOARS.
    10.1016/j.celrep.2016.06.084
  26. Liepelt, A.; Naarmann-De Vries, I.S.; Simons, N.; Eichelbaum, K.; Föhr, S.; Archer, S.K.; Castello, A.; et al. "Identification of RNA-binding proteins in macrophages by interactome capture". Molecular and Cellular Proteomics 15 8 (2016): 2699-2714. http://www.scopus.com/inward/record.url?eid=2-s2.0-84982839157&partnerID=MN8TOARS.
    10.1074/mcp.M115.056564
  27. Castello, A.; Hentze, M.W.; Preiss, T.. "Metabolic Enzymes Enjoying New Partnerships as RNA-Binding Proteins". Trends in Endocrinology and Metabolism 26 12 (2015): 746-757. http://www.scopus.com/inward/record.url?eid=2-s2.0-84949547310&partnerID=MN8TOARS.
    10.1016/j.tem.2015.09.012
  28. Beckmann BM; Horos R; Fischer B; Castello A; Eichelbaum K; Alleaume AM; Schwarzl T; et al. "The RNA-binding proteomes from yeast to man harbour conserved enigmRBPs.". (2015): http://europepmc.org/abstract/med/26632259.
    10.1038/ncomms10127
  29. Castello, A.. "The emerging universe of RNA-binding proteins". Biochemist 37 2 (2015): 33-38. http://www.scopus.com/inward/record.url?eid=2-s2.0-84928987190&partnerID=MN8TOARS.
  30. C. Strein; A.-M. Alleaume; U. Rothbauer; M. W. Hentze; A. Castello. "A versatile assay for RNA-binding proteins in living cells". RNA 20 5 (2014): 721-731. http://dx.doi.org/10.1261/rna.043562.113.
    10.1261/rna.043562.113
  31. Enrique Álvarez; Alfredo Castelló; Luis Carrasco; José M. Izquierdo; Emanuele Buratti. "Poliovirus 2A Protease Triggers a Selective Nucleo-Cytoplasmic Redistribution of Splicing Factors to Regulate Alternative Pre-mRNA Splicing". PLoS ONE 8 9 (2013): e73723-e73723. http://dx.doi.org/10.1371/journal.pone.0073723.
    10.1371/journal.pone.0073723
  32. S Chul Kwon; Hyerim Yi; Katrin Eichelbaum; Sophia Föhr; Bernd Fischer; Kwon Tae You; Alfredo Castello; et al. "The RNA-binding protein repertoire of embryonic stem cells". Nat Struct Mol Biol 20 9 (2013): 1122-1130. http://dx.doi.org/10.1038/nsmb.2638.
    10.1038/nsmb.2638
  33. Alfredo Castello; Bernd Fischer; Matthias W. Hentze; Thomas Preiss. "RNA-binding proteins in Mendelian disease". Trends in Genetics 29 5 (2013): 318-327. http://dx.doi.org/10.1016/j.tig.2013.01.004.
    10.1016/j.tig.2013.01.004
  34. Elena G. Sánchez; Ana Quintas; Marisa Nogal; Alfredo Castelló; Yolanda Revilla. "African swine fever virus controls the host transcription and cellular machinery of protein synthesis". Virus Research 173 1 (2013): 58-75. http://dx.doi.org/10.1016/j.virusres.2012.10.025.
    10.1016/j.virusres.2012.10.025
  35. Alfredo Castello; Rastislav Horos; Claudia Strein; Bernd Fischer; Katrin Eichelbaum; Lars M Steinmetz; Jeroen Krijgsveld; Matthias W Hentze. "System-wide identification of RNA-binding proteins by interactome capture". Nat Protoc 8 3 (2013): 491-500. http://dx.doi.org/10.1038/nprot.2013.020.
    10.1038/nprot.2013.020
  36. Javier del Pino; José Luis Jiménez; Iván Ventoso; Alfredo Castelló; Ma. Ángeles Muñoz-Fernández; César de Haro; Juan José Berlanga; Jean-Luc E. P. H. Darlix. "GCN2 Has Inhibitory Effect on Human Immunodeficiency Virus-1 Protein Synthesis and Is Cleaved upon Viral Infection". PLoS ONE 7 10 (2012): e47272-e47272. http://dx.doi.org/10.1371/journal.pone.0047272.
    10.1371/journal.pone.0047272
  37. Alfredo Castello; Bernd Fischer; Katrin Eichelbaum; Rastislav Horos; Benedikt M. Beckmann; Claudia Strein; Norman E. Davey; et al. "Insights into RNA Biology from an Atlas of Mammalian mRNA-Binding Proteins". Cell 149 6 (2012): 1393-1406. http://dx.doi.org/10.1016/j.cell.2012.04.031.
    10.1016/j.cell.2012.04.031
  38. M. J. Buzon; I. Erkizia; C. Pou; G. Minuesa; M. C. Puertas; A. Esteve; A. Castello; et al. "A non-infectious cell-based phenotypic assay for the assessment of HIV-1 susceptibility to protease inhibitors". Journal of Antimicrobial Chemotherapy 67 1 (2011): 32-38. http://dx.doi.org/10.1093/jac/dkr433.
    10.1093/jac/dkr433
  39. Enrique Álvarez; Alfredo Castelló; Luis Carrasco; José M. Izquierdo. "Alternative splicing, a new target to block cellular gene expression by poliovirus 2A protease". Biochemical and Biophysical Research Communications 414 1 (2011): 142-147. http://dx.doi.org/10.1016/j.bbrc.2011.09.040.
    10.1016/j.bbrc.2011.09.040
  40. Emilio Yángüez; Alfredo Castello; Ewelina Welnowska; Luis Carrasco; Ian Goodfellow; Amelia Nieto. "Functional impairment of eIF4A and eIF4G factors correlates with inhibition of influenza virus mRNA translation". Virology 413 1 (2011): 93-102. http://dx.doi.org/10.1016/j.virol.2011.02.012.
    10.1016/j.virol.2011.02.012
  41. Alfredo Castelló; Enrique Álvarez; Luis Carrasco. "The Multifaceted Poliovirus 2A Protease: Regulation of Gene Expression by Picornavirus Proteases". Journal of Biomedicine and Biotechnology 2011 (2011): 1-23. http://dx.doi.org/10.1155/2011/369648.
    10.1155/2011/369648
  42. Alfredo Castelló; David Franco; Pablo Moral-López; Juan J. Berlanga; Enrique Álvarez; Eckard Wimmer; Luis Carrasco; Douglas F. Nixon. "HIV- 1 Protease Inhibits Cap- and Poly(A)-Dependent Translation upon eIF4GI and PABP Cleavage". PLoS ONE 4 11 (2009): e7997-e7997. http://dx.doi.org/10.1371/journal.pone.0007997.
    10.1371/journal.pone.0007997
  43. A. Castello; J. M. Izquierdo; E. Welnowska; L. Carrasco. "RNA nuclear export is blocked by poliovirus 2A protease and is concomitant with nucleoporin cleavage". Journal of Cell Science 122 20 (2009): 3799-3809. http://dx.doi.org/10.1242/jcs.055988.
    10.1242/jcs.055988
  44. Alfredo Castelló; Ana Quintas; Elena G. Sánchez; Prado Sabina; Marisa Nogal; Luis Carrasco; Yolanda Revilla; Ian Mohr. "Regulation of Host Translational Machinery by African Swine Fever Virus". PLoS Pathog 5 8 (2009): e1000562-e1000562. http://dx.doi.org/10.1371/journal.ppat.1000562.
    10.1371/journal.ppat.1000562
  45. Miguel Ángel Sanz; Alfredo Castelló; Iván Ventoso; Juan José Berlanga; Luis Carrasco; Wang-Shick Ryu. "Dual Mechanism for the Translation of Subgenomic mRNA from Sindbis Virus in Infected and Uninfected Cells". PLoS ONE 4 3 (2009): e4772-e4772. http://dx.doi.org/10.1371/journal.pone.0004772.
    10.1371/journal.pone.0004772
  46. Ewelina Welnowska; Alfredo Castelló; Pablo Moral; Luis Carrasco. "Translation of mRNAs from Vesicular Stomatitis Virus and Vaccinia Virus Is Differentially Blocked in Cells with Depletion of eIF4GI and/or eIF4GII". Journal of Molecular Biology 394 3 (2009): 506-521. http://dx.doi.org/10.1016/j.jmb.2009.09.036.
    10.1016/j.jmb.2009.09.036
  47. Vanessa Madan; Alfredo Castelló; Luis Carrasco. "Viroporins from RNA viruses induce caspase-dependent apoptosis". Cell Microbiol 0 0 (2007): 071027034427002-???. http://dx.doi.org/10.1111/j.1462-5822.2007.01057.x.
    10.1111/j.1462-5822.2007.01057.x
  48. Susana Molina; Miguel A. Sanz; Vanesa Madan; Iván Ventoso; Alfredo Castelló; Luis Carrasco. "Differential inhibition of cellular and Sindbis virus translation by brefeldin A". Virology 363 2 (2007): 430-436. http://dx.doi.org/10.1016/j.virol.2007.02.001.
    10.1016/j.virol.2007.02.001
  49. M. A. Sanz; A. Castello; L. Carrasco. "Viral Translation Is Coupled to Transcription in Sindbis Virus-Infected Cells". Journal of Virology 81 13 (2007): 7061-7068. http://dx.doi.org/10.1128/jvi.02529-06.
    10.1128/jvi.02529-06
  50. Sanz, M.A.; Castelló, A.; Carrasco, L.. "Viral translation is coupled to transcription in sindbis virus-infected cells". Journal of Virology 81 13 (2007): 7061-7068. http://www.scopus.com/inward/record.url?eid=2-s2.0-34250879518&partnerID=MN8TOARS.
    10.1128/JVI.02529-06
  51. A. Castello; E. Alvarez; L. Carrasco. "Differential Cleavage of eIF4GI and eIF4GII in Mammalian Cells: EFFECTS ON TRANSLATION". Journal of Biological Chemistry 281 44 (2006): 33206-33216. http://dx.doi.org/10.1074/jbc.m604340200.
    10.1074/jbc.m604340200
  52. Enrique Álvarez; Alfredo Castelló; Luis Menéndez-Arias; Luis Carrasco. "HIV protease cleaves poly(A)-binding protein". Biochem. J. 396 2 (2006): 219-226. http://dx.doi.org/10.1042/bj20060108.
    10.1042/bj20060108
  53. Alfredo Castelló; Miguel Ángel Sanz; Susana Molina; Luis Carrasco. "Translation of Sindbis Virus 26S mRNA Does Not Require Intact Eukariotic Initiation Factor 4G". Journal of Molecular Biology 355 5 (2006): 942-956. http://dx.doi.org/10.1016/j.jmb.2005.11.024.
    10.1016/j.jmb.2005.11.024
  54. Álvarez, E.; Castelló, A.; Menéndez-Arias, L.; Carrasco, L.. "HIV protease cleaves poly(A)-binding protein". Biochemical Journal 396 2 (2006): 219-226. http://www.scopus.com/inward/record.url?eid=2-s2.0-33744806877&partnerID=MN8TOARS.
    10.1042/BJ20060108
  55. Castello, A.; Alvarez, E.; Carrasco, L.. "Differential cleavage of eIF4GI and eIF4GII in mammalian cells: Effects on translation". Journal of Biological Chemistry 281 44 (2006): 33206-33216. http://www.scopus.com/inward/record.url?eid=2-s2.0-33845931570&partnerID=MN8TOARS.
    10.1074/jbc.M604340200

Other

Other output
  1. CryoSIM: super resolution 3D structured illumination cryogenic fluorescence microscopy for correlated ultra-structural imaging. 2020. Michael A. Phillips; Maria Harkiolaki; David Miguel Susano Pinto; Richard M. Parton; Ana Palanca; Manuel Garcia-Moreno; Ilias Kounatidis; et al. https://doi.org/10.1101/2020.03.09.980334.
    10.1101/2020.03.09.980334
  2. System-wide analyses of the fission yeast poly(A)+ RNA interactome reveal insights into organisation and function of RNA-protein complexes. 2019. Cornelia Kilchert; Tea Kecman; Emily Priest; Svenja Hester; Krzysztof Kus; Alfredo Castello; Shabaz Mohammed; Lidia Vasiljeva. https://doi.org/10.1101/748194.
    10.1101/748194
  3. RNA-binding activity of TRIM25 is mediated by its PRY/SPRY domain and is required for ubiquitination. 2017. Nila Roy Choudhury; Gregory Heikel; Maryia Trubitsyna; Peter Kubik; Jakub Stanislaw Nowak; Shaun Webb; Sander Granneman; et al. https://doi.org/10.1101/200410.
    10.1101/200410