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Ana do Vale graduated in Aquatic Sciences from the University of Porto in 1996 and received her PhD from the same University in 2003. She worked at the University of Santiago de Compostela (Spain) and Marine Laboratory (Aberdeen, UK). Since 2004 she integrates the Fish Immunology and Vaccinology Group of IBMC/i3S-Universidade do Porto and was/is the PI of several projects (funded in national and international competitive calls) within the group. Ana do Vale has a solid background on molecular and cellular microbiology and fish biology. She has a long-standing interest in bacterial virulence and her work contributed to identify several virulence mechanisms of Photobacterium damselae subsp. piscicida (Phdp), a Gram-negative bacterium that causes a devastating septicemia in several wild and cultured marine fish species. She is currently very committed to apply her recent findings on novel virulence factors of Phdp to develop an effective subunit vaccine against that pathogen. Ana do Vale has experience in implementing and managing R&D projects and in supervising undergraduate, MSc and PhD students. She co-authored 2 patents and published numerous articles in peer-reviewed scientific journals.
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Identification

Personal identification

Full name
Ana Maria Silva do Vale

Citation names

  • do Vale, Ana

Author identifiers

Ciência ID
661E-CBF2-6AA5
ORCID iD
0000-0002-4044-6328

Email addresses

  • avale@ibmc.up.pt (Professional)
  • avale@i3s.up.pt (Professional)

Telephones

Telephone
  • 220408800 (Professional)

Addresses

  • i3S, Instituto de Investigação e Inovação em Saúde. Rua Alfredo Allen, 208 , 4200-135 P, Porto, Porto, Portugal (Professional)
  • IBMC, Instituto de Biologia Molecular e Celular. Rua Alfredo Allen, 208, 4200-135, Porto, Porto, Portugal (Professional)

Knowledge fields

  • Natural sciences - Biological Sciences - Microbiology
  • Natural sciences - Biological Sciences - Cell Biology
  • Agrarian Sciences - Veterinary Sciences

Languages

Language Speaking Reading Writing Listening Peer-review
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Spanish; Castilian Advanced (C1) Advanced (C1) Intermediate (B1) Advanced (C1) Advanced (C1)
French Beginner (A1) Beginner (A1) Beginner (A1) Beginner (A1) Beginner (A1)
Galician Intermediate (B1) Advanced (C1) Beginner (A1) Intermediate (B1) Advanced (C1)
Education
Degree Classification
2011/05/02 - 2011/05/13
Concluded
 Course on laboratory Animal Science, Category C (80 hours), accredited by FELASA (Outros)
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
1997/09/01 - 2003/12/31
Concluded
 Aquatic Sciences (Doutoramento)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
"Anti-phagocytic mechanisms of Photobacterium damselae subsp. piscicida in sea bass pasteurellosis" (THESIS/DISSERTATION)
1991 - 1996/10/22
Concluded
 Aquatic Sciences (Licenciatura)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
"In vivo expression of the 70 kDa Extracellular protease and of the iron regulated outer membrane proteins of the fish pathogen Aeromonas salmonicida" (THESIS/DISSERTATION)
 16/20
Affiliation

Science

Category
Host institution 		Employer
2022/08/16 - Current Auxiliary Researcher (Research) Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2019/01/01 - 2022/08/15 Researcher (Research) Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2014/07/15 - 2018/12/31 Postdoc (Research) Fundação para a Ciência e a Tecnologia, Portugal
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2009/07/01 - 2014/06/30 Contracted Researcher (Research) Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2007/01/01 - 2009/06/30 Postdoc (Research) Fundação para a Ciência e a Tecnologia, Portugal
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2007 - 2008 Postdoc (Research) Fundação para a Ciência e a Tecnologia, Portugal
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2004/01/01 - 2006/12/31 Postdoc (Research) Fundação para a Ciência e a Tecnologia, Portugal
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
1996/01/01 - 1996/06/30 Research Trainee (Research) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
University of Aberdeen, United Kingdom

Teaching in Higher Education

Category
Host institution 		Employer
2012/01/01 - 2014/07/31 Invited Adjunct Teacher (Polytechnic Teacher) Instituto Politécnico do Porto Escola Superior de Saúde, Portugal
Instituto Politécnico do Porto Escola Superior de Saúde, Portugal
2004 - 2006 Invited Adjunct Teacher (Polytechnic Teacher) Instituto Superior de Saúde do Alto Ave, Portugal
Instituto Superior de Saúde do Alto Ave, Portugal

Others

Category
Host institution 		Employer
1997/09/01 - 2003/12/31 PhD student Fundação para a Ciência e a Tecnologia, Portugal
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
(...)
Projects

Grant

Designation Funders
2015/03 - 2018/12 Structural determinants required for receptor binding and membrane translocation of AIP56, an AB toxin targeting NF-kB
Provided by PTCRIS: SFRH/BPD/95777/2013
Principal investigator
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2007/01 - 2009/06 STUDIES ON THE ROLE OF THE AIP56 EXOTOXIN IN THE PATHOGENESIS AND DISSEMINATION OF FISH SEPTICEMIC PASTEURELLOSIS
Provided by PTCRIS: SFRH/BPD/26816/2006
Principal investigator
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
 Fundação para a Ciência e a Tecnologia
Cancelled
2004/01 - 2006/12 PROGRAMMED CELL DEATH INDUCED IN SEA BASS BY PHOTOBACTERIUM DAMSELAE SUBSP. PISCICIDA INFECTION: STUDIES TOWARDS IDENTIFICATION OF THE APOPTOGENIC FACTOR, ELUCIDATION OF APOPTOTIC PATHWAYS AND DEVELOPMENT OF AN ANTI-PHOTOBACTERIUM VACCINE
Provided by PTCRIS: SFRH/BPD/11538/2002
 Fundação para a Ciência e a Tecnologia
1997/09 - 2001/08 VIRULENCE FACTORS AND MECHANISMS OF PHOTOBACTERIUM DAMSELA, THE CAUSATIVE AGENT OF PASTEURELLOSIS
Provided by PTCRIS: PRAXIS XXI/BD/11038/97
 Fundação para a Ciência e a Tecnologia

Contract

Designation Funders
2022/11/01 - Current IGNITION - Improving Green Innovation for the Blue Revolution: new tools and opportunities for a more sustainable animal farming (https://ignition-project.eu/) (PI at i3S; i3S budget-356,591 euros; total budget- 5,077,442 euros)
101084651
Principal investigator
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
 Horizon 2020
Ongoing
2022/08/16 - 2028/08/15 VIVACE: Prevention of infectious diseases in aquaculture: exploring VIrulence factors for the development of subunit VACcinEs for fish
2021.02251.CEECIND/CP1663/CT0016
10.54499/2021.02251.CEECIND/CP1663/CT0016
Principal investigator
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2021/01/01 - 2025/12/31 Institute for Research and Innovation in Health
LA/P/0070/2020
Instituto Nacional de Engenharia Biomédica, Portugal

Universidade do Porto, Portugal

Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal

Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal

Universidade do Porto Instituto de Patologia e Imunologia Molecular, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2021/03/28 - 2025/03/27 T3SS and T5SS in Photobacterium damselae subsp. piscicida: from basic studies to vaccine development
PTDC/CVT-CVT/7177/2020
10.54499/PTDC/CVT-CVT/7177/2020
Principal investigator
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2019/01/01 - 2022/08/15 Research Contract (https://doi.org/10.54499/DL57/2016/CP1355/CT0010)
DL 57/2016/CP1355/CT0010
10.54499/DL57/2016/CP1355/CT0010
Principal investigator
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2018/07/15 - 2022/07/14 Identification and characterization of virulence factors secreted by Photobacterium damselae piscicida with potential to be used as antigens for vaccination against fish photobacteriosis (PI; Funding 232,362 euros)
PTDC/CVT-CVT/30018/2017
Principal investigator
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2018/07/15 - 2022/07/14 Structural and functional characterization of AB toxins targeting antigen-presenting cells and their application as cancer vaccines (Co-PI; Funding 235,831 euros)
PTDC/BIA-MIC/29910/2017
Co-Principal Investigator (Co-PI)
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2019/01/01 - 2019/12/31 Instituto de Investigação e Inovação em Saúde
UID/BIM/04293/2019
Instituto Nacional de Engenharia Biomédica, Portugal

Universidade do Porto, Portugal

Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal

Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal

Universidade do Porto Instituto de Patologia e Imunologia Molecular, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2016/06/01 - 2019/11/30 Host cell factors and molecular mechanisms implicated in intoxication by AIP56, a bacterial toxin targeting NF-kB (PI; Funding 198,048 euros)
PTDC/BIA-MIC/2007/2014
Principal investigator
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2013/05/15 - 2015/09/30 Studies towards identification of structural determinants required for receptor binding and membrane translocation of AIP56, a Photobacterium damselae subsp. piscicida apoptogenic AB toxin targeting NF-kB (PI; Funding 195,655 euros)
129800
PTDC/BIA-MIC/3463/2012
Provided by PTCRIS: 129800
Principal investigator
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
 Fundação para a Ciência e a Tecnologia, I.P.
Concluded
2010/06/01 - 2013/10/31 Cell entry and intracellular trafficking of the apoptogenic AIP56 exotoxin, a key virulence factor of Photobacterium damselae subsp. piscicida (PI; Funding 153,402 euros)
99544
PTDC/CVT/099544/2008
Provided by PTCRIS: 99544
Principal investigator
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
 Fundação para a Ciência e a Tecnologia, I.P.
Concluded
2010/05/01 - 2013/10/31 Rational design of an AIP56-based anti-pasteurellosis vaccine
101143
PTDC/MAR/101143/2008
Provided by PTCRIS: 101143
Researcher
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
 Fundação para a Ciência e a Tecnologia, I.P.
Concluded
2010/05/01 - 2013/10/31 The role of iron-related acute phase proteins during bacterial infection in fish
100386
PTDC/CVT/100386/2008
Provided by PTCRIS: 100386
Researcher
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
 Fundação para a Ciência e a Tecnologia, I.P.
Concluded
2011/01/01 - 2012/12/31 Strategic Project - LA 2 - 2011-2012
PEst-C/SAU/LA0002/2011
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal

Instituto Nacional de Engenharia Biomédica, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2010/04/01 - 2012/01/31 Biochemical and biophysical characterization of AIP56, a novel bacterial apoptogenic exotoxin
101111
PTDC/BIA-PRO/101111/2008
Provided by PTCRIS: 101111
Researcher
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
 Fundação para a Ciência e a Tecnologia, I.P.
Concluded
2007/10/01 - 2011/06/30 Creating tools for studying sea bass (Dicentrarchus labrax) immunity
69086
PTDC/CVT/69086/2006
Provided by PTCRIS: 69086
Researcher
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
 Fundação para a Ciência e a Tecnologia, I.P.
Concluded
2005/11/01 - 2009/01/31 Studies on the apoptosis induced in sea bass (Dicentrarchus labrax L.) phagocytes by Photobacterium damselae subsp. piscicida exotoxin AIP56 (PI; Funding 98,753 euros)
56111
POCI/MAR/56111/2004
Provided by PTCRIS: 56111
Principal investigator
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
 Fundação para a Ciência e a Tecnologia, I.P.
Concluded
2005/01/01 - 2008/06/30 Apoptotic pathway(s) in Saccharomyces cerevisiae: proteomic profile and metacaspase-interactive molecules
57364
POCI/BIA-BCM/57364/2004
Provided by PTCRIS: 57364
Researcher
Universidade do Minho Instituto de Investigação em Ciências da Vida e Saúde, Portugal
 Fundação para a Ciência e a Tecnologia, I.P.
Concluded
2003/09/15 - 2006/09/14 Development of a vaccine against Photobacterium infection (PI; Funding 50,000 euros)
XX
Principal investigator
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
Concluded
Outputs

Publications

Conference poster
  1. Molina-Sorribes, C; Lisboa, Johnny; Sanjuan, E; Fouz, B; do Vale, Ana; Amaro, C. "A NEW VACCINE AGAISNT THE ZOONOTIC PATHOGEN Vibrio vulnificus: FIRST STEP". Paper presented in Aquaculture Europe 2023, 18-21 Sepetember 2023, Viena, Austria, 2023.
  2. Pinto, Alexandre Jorge; Lisboa, Johnny; Barca, Alba V.; Terceti, Mateus S.; Azevedo, Jorge E; Osorio, Carlos; dos Santos, Nuno M.S.; do Vale, Ana. "The two-component system RstAB regulates toxin expression in Photobacterium damselae subsp. piscicida". Paper presented in ETOX 2023-EUROPEAN WORKSHOP ON BACTERIAL PROTEIN TOXINS, June 25 - June 29 2023, Crieff, UK, 2023.
  3. Bruno Pinheiro; Moura, Ana; Lisboa, Johnny; Azevedo, Jorge E; do Vale, Ana; dos Santos, Nuno M.S.. "Development of tools towards the use of AIP56 as a vehicle for the intracellular delivery of antigens in antigen-presenting cells". Paper presented in ETOX 2023-EUROPEAN WORKSHOP ON BACTERIAL PROTEIN TOXINS, June 25 - June 29 2023, Crieff, UK, 2023.
  4. Lisboa, Johnny; Freitas, Inês Lua; Pereira, Cassilda; Bruno Pinheiro; D. Pinto, Rute; S. Rodrigues, Inês; Pereira, Liliana M.G.; et al. "Three-dimensional structure and mechanisms of membrane interaction and translocation of the NFkB-targeting toxin AIP56". Paper presented in ETOX 2023-EUROPEAN WORKSHOP ON BACTERIAL PROTEIN TOXINS, June 25 - June 29 2023, Crieff, UK, 2023.
  5. Freitas, Inês Lua; Macedo, Fatima; Oliveira, Liliana; do Vale, Ana; dos Santos, Nuno M.S.. "The NF-kB targeting toxin AIP56 has tropism for myeloid cells". Paper presented in ETOX 2023-EUROPEAN WORKSHOP ON BACTERIAL PROTEIN TOXINS, June 25 - June 29 2023, Crieff, UK, 2023.
  6. José Viveiros; Lisboa, Johnny; Teixeira, Alexandra; Loureiro, Inês; dos Santos, Nuno M.S.; do Vale, Ana. "Recombinant expression and purification of trimeric autotransporter adhesins from Photobacterium damselae subsp. piscicida". Paper presented in IJUP 2023, 10-12 Maio 2023, Porto, Portugal, 2023.
  7. Bruno Pinheiro; Moura, Ana; Lisboa, Johnny; Azevedo, Jorge E; do Vale, Ana; dos Santos, Nuno M.S.. "Development of tools towards the use of AIP56 as a vehicle for the intracellular delivery of antigens in antigen-presenting cells". Paper presented in XLVIII Annual Meeting of the Portuguese Society for Immunology, 29-31 March 2023, Aveiro, Portugal, 2023.
  8. Freitas, Inês Lua; Macedo, Fatima; Oliveira, Liliana; do Vale, Ana; dos Santos, Nuno M.S.. "Cellular Specificity of AIP56, a Bacterial Toxin that Targets NF-¿B". Paper presented in XLVIII Annual Meeting of the Portuguese Society for Immunology, 29-31 March 2023, Aveiro, Portugal, 2023.
  9. Lisboa, Johnny; Freitas, Inês Lua; do Vale, Ana; dos Santos, Nuno M.S.. "AIP56 and AIP56-like toxins: a family of bacterial toxins that target NF-kB". Paper presented in XLVIII Annual Meeting of the Portuguese Society for Immunology, 29-31 March 2023, Aveiro, Portugal, 2023.
  10. Pinheiro, Bruno; Moura, Ana; Lisboa, Johnny; Azevedo, Jorge E; do Vale, Ana; dos Santos, Nuno M.S.. "Development of tools towards the use of AIP56 as a vehicle for the intracellular delivery of antigens in antigen-presenting cells". Paper presented in I ImmunoHUB Annual Meeting - Infection & Immunity. i3S - Instituto de Investigação e Inovação em Saúde, Porto, Portugal, 2023.
  11. Freitas, Inês Lua; Macedo, Fatima; Oliveira, Liliana; do Vale, Ana; dos Santos, Nuno M.S.. "Cellular Specificity of AIP56, a Bacterial Toxin that Targets NF-kB". Paper presented in I ImmunoHUB Annual Meeting - Infection & Immunity. i3S - Instituto de Investigação e Inovação em Saúde, Porto, Portugal, 2023.
  12. Freitas, Inês Lua; Macedo, M Fatima; Oliveira, Liliana; do Vale, Ana; dos Santos, Nuno M.S.. "AIP56 Has Tropism for Myeloid Cells". Paper presented in 9th McBiology Symposium, 23rd and 24th February 2023, i3S, Porto, Portugal, 2023.
  13. Pinheiro, Bruno; Moura, Ana; Lisboa, Johnny; Azevedo, Jorge E; do Vale, Ana; dos Santos, Nuno M.S.. "Antigen Delivery by AB toxins". Paper presented in 9th McBiology Symposium, 23rd and 24th February 2023, i3S, Porto, Portugal, 2023.
  14. Ferreira, Inês; Guardiola, Francisco A.; do Vale, Ana; Costas, Benjamin. "Early mucosal immune responses in European seabass (Dicentrarchus labrax) following Tenacibaculum maritimum infection. IX Animal Science Workshop ICBAS, Porto, Portugal. 15th of September, 2022.". Paper presented in IX Animal Science Workshop, ICBAS, Porto, Portugal, 2022.
  15. Lisboa, Johnny; D. Pinto, Rute; Rodrigues, Inês; Pereira, Liliana M.G.; Pereira, Cassilda; Oliveira, Pedro; Barbosa Pereira, Pedro J.; et al. "3D structure of the NFkB-targeting toxin AIP56 and characterization of determinants required for low pH-induced conformational changes, membrane interaction and translocation". Paper presented in IUBMB-FEBS-PABMB 2022 Congress: The Biochemistry Global Summit, Lisbon, 9-14 July 2022, 2022.
  16. Pinto, Alexandre Jorge; Lisboa, Johnny; dos Santos, Nuno M.S.; do Vale, Ana. "Role of RstAB in the regulation of toxin expression by Photobacterium damselae subsp. piscicida". Paper presented in 8th Molecular and Cell Biology Symposium, 2022, 2022.
  17. Lisboa, Johnny; Inês Lua Freitas; do Vale, Ana; dos Santos, Nuno M.S.. "Structural and functional characterization of NiTox: a new AB-toxin from Vibrio nigripulchritudo lethal to shrimp". Paper presented in ETOX2021-EUROPEAN WORKSHOP ON BACTERIAL PROTEIN TOXINS, 2021.
  18. Lisboa, Johnny; Freitas, Inês Lua; do Vale, Ana; dos Santos, Nuno M.S.. "NiTox: a new Vibrio Nigripulchritudo AB-toxin". Paper presented in ETOX 21 - European Workshop on Bacterial Protein Toxins, 2021.
  19. Ferreira, Inês; Guardiola, Francisco A.; do Vale, Ana; Costas, Benjamin. "Tenacibaculum maritimum pathogenesis: crosstalk between host and pathogen and beyond". Paper presented in Blue Think Conference - Share Science, Spread Knowledge. CIIMAR, Porto, Portugal, 2020.
  20. Ferreira, Inês; Guardiola, Francisco A.; do Vale, Ana; Costas, Benjamin. "Tenacibaculum maritimum pathogenesis: crosstalk between host and pathogen and beyond". Paper presented in VII Animal Science Workshop, ICBAS, Porto, Portugal, 2020.
  21. Teixeira, Alexandra; Loureiro, Inês; dos Santos, Nuno M.S.; do Vale, Ana. "Outer Membrane Vesicles from Photobacterium damselae subsp. piscicida: characterization and antigenic potential". Paper presented in ISEV2020 Virtual Annual Meeting, 2020.
  22. Teixeira, Alexandra; Loureiro, Inês; dos Santos, Nuno M.S.; do Vale, Ana. "Isolation and characterization of Outer Membrane Vesicles from Photobacterium damselae subsp. piscicida". Paper presented in i3S Annual Meeting, 28-29 November 2019, Póvoa de Varzim, Portugal, 2019.
  23. Loureiro, Inês; Teixeira, Alexandra; Lisboa, Johnny; Barca, Alba V.; Osorio, Carlos; Gärtner, F.; dos Santos, Nuno M.S.; do Vale, Ana. "Photobacterium deadly duet: uncovering the mechanism of action of a putative binary pore-forming toxin". Paper presented in i3S Annual Meeting, 28-29 November 2019, Póvoa de Varzim, Portugal, 2019.
  24. Lisboa, Johnny; Fernandes, Andreia; Freitas, Inês Lua; do Vale, Ana; dos Santos, Nuno M.S.. "Structural Insights into AIP56 and AIP56-related toxins". Paper presented in i3S Annual Meeting, 28-29 November 2019, Póvoa de Varzim, Portugal, 2019.
  25. Lisboa, Johnny; Fernandes, Andreia; Freitas, Inês Lua; do Vale, Ana; dos Santos, Nuno M.S.. "Structural Insights on AIP56 and AIP56-relarted Toxins". Paper presented in ETOX19, European Workshop on Bacterial protein Toxins, Davos, Switzerland, 22-26 June 2019, 2019.
  26. Lisboa, Johnny; Pereira, Cassilda; Ayala, Juan; Terceti, Mateus; Rodrigues, Inês; Pereira, Pedro J.B.; Osorio, Carlos R.; et al. "Characterization of a novel NlpC/P60 endopeptidase of Photobacterium damselae subsp. piscicida secreted by the Type II secretion system". Paper presented in Bacterial Protein Export 2018, 30 September - 03 October 2018, Leuven, Belgium., 2018.
  27. Rodrigues, Inês; Pereira, Cassilda; Pereira, Liliana M.G.; Lisboa, Johnny; dos Santos, Nuno M.S.; do Vale, Ana. "Host factors involved in AIP56 intoxication: the role of protein chaperones and redox systems". Paper presented in ETOX18- 18th European Workshop on Bacterial Protein Toxins, 26-30 June 2017, Paris, France., 2017.
  28. Lisboa, Johnny; Rodrigues, Inês; Benz, Roland; do Vale, Ana; dos Santos, Nuno M.S.. "Structural determinants required for pH-induced unfolding and pore formation in AIP56". Paper presented in ETOX18- 18th European Workshop on Bacterial Protein Toxins, 26-30 June 2017, Paris, France., 2017.
  29. Pereira, Liliana M.G.; Pinto, Rute D.; Peres, José; Rodrigues, Inês; Lisboa, Johnny; dos Santos, Nuno M.S.; do Vale, Ana. "Involvement of Hsp90 and PPIases in AIP56 intoxication". Paper presented in ETOX17- 17th European Workshop on Bacterial Protein Toxins, 20-24 June 2015, Braga, Portugal., 2015.
  30. Peres, José; Rodrigues, Inês; Pereira, Liliana M.G.; Pinto, Rute D.; Lisboa, Johnny; do Vale, Ana; dos Santos, Nuno M.S.. "AIP56: a short-trip AB toxin with a peculiar translocation domain". Paper presented in ETOX17- 17th European Workshop on Bacterial Protein Toxins, 20-24 June 2015, Braga, Portugal., 2015.
  31. Pereira, Liliana M.G.; Silva, Daniela S.P.; Moreira, Ana R.; Sampaio, Paula; dos Santos, Nuno M.S.; do Vale, Ana. "Trafficking of AIP56 in mouse bone marrow-derived macrophages". Paper presented in 16th European Workshop on Bacterial Protein Toxins, 22-26 June 2013, Freiburg, Germany., 2013.
  32. Silva, Daniela S.P.; Pereira, Liliana M.G.; Moreira, Ana R.; Araújo, Leonor; do Vale, Ana; dos Santos, Nuno M.S.. "AIP56 and NleC: structural and functional conservation but different unfolding properties". Paper presented in 16th European Workshop on Bacterial Protein Toxins, 22-26 June 2013, Freiburg, Germany., 2013.
  33. Silva, Daniela S.P.; Pereira, Liliana M.G.; Silva, Frederico; do Vale, Ana; dos Santos, Nuno M.S.. "AIP56, an apoptogenic AB-toxin targeting NF-kB p65 (Winner of Best Poster Prize)". Paper presented in 15th European Workshop on Bacterial Protein Toxins, 18-22 June 2011, Oslo, Norway., 2011.
  34. Pereira, Liliana M.G.; Silva, Daniela S.P.; Beitzinger, C.; Benz, Roland; dos Santos, Nuno M.S.; do Vale, Ana. "Is AIP56 translocated from endosomes?". Paper presented in 15th European Workshop on Bacterial Protein Toxins, 18-22 June 2011, Oslo, Norway., 2011.
  35. Pereira, Liliana M.G.; Costa-Ramos, Carolina; dos Santos, Nuno M.S.; do Vale, Ana. "Studies on the interaction of AIP56, a novel bacterial apoptogenic exotoxin, with host cells". Paper presented in 14th European Workshop on Bacterial Protein Toxins, Obernai, France, June 27-Jully 02, 2009., 2009.
  36. do Vale, Ana; Costa-Ramos, Carolina; Pereira, Liliana M.G.; Silva, Manuel T.; dos Santos, Nuno M.S.. "Phagocyte apoptosis induced by the bacterial exotoxin AIP56 involves toxin internalization and the participation of mitochondria". Paper presented in ESH Conference; MECHANISMS OF CELL DEATH AND DISEASE: ADVANCES IN THERAPEUTIC INTERVENTION AND DRUG DEVELOPMENT, Cascais, Portugal, October 30 - November 04, 2008., 2008.
  37. Costa-Ramos, Carolina; do Vale, Ana; Silva, Manuel T.; dos Santos, Nuno M.S.. "Studies on apoptosis induced by AIP56, a novel virulence factor of Photobacterium damselae ssp piscicida". Paper presented in 16th Euroconference on Apoptosis and 5th Swiss Apoptosis Meeting, Bern, Switzerland, September 6-9, 2008., 2008.
  38. Reis, Marta I.R.; do Vale, Ana; dos Santos, Nuno M.S.. "Molecular cloning and characterisation of sea bass (Dicentrarchus labrax)caspase-8". Paper presented in 16th Euroconference on Apoptosis and 5th Swiss Apoptosis Meeting, Bern, Switzerland, September 6-9, 2008., 2008.
  39. do Vale, Ana; Costa-Ramos, Carolina; Silva, Daniela S.P.; dos Santos, Nuno M.S.; Silva, Manuel T.. "Brush border microvillar loss during detachment-triggered apoptosis (anoikis) of enterocytes.". Paper presented in XLI Congress of the Portuguese Society for Microscopy, Braga, Portugal, December 14-15, 2006., 2006.
  40. Nascimento, Diana S; do Vale, Ana; Tomás, Ana M.; Zou, Jun; Secombes, Christopher J.; dos Santos, Nuno M.S.. "Cloning, promoter analysis and expression of sea bass (Dicentrarchus labrax L.) interleukin-12 p40 and p35 subunits.". Paper presented in XXXIII Annual Meeting of the Sociedade Portuguesa de Imunologia, Braga, Portugal, 13th and 14th October 2006., 2006.
  41. Pinto, Rute D.; Nascimento, Diana S.; Reis, Marta I.R.; do Vale, Ana; dos Santos, Nuno M.S.. "Molecular characterization, 3D modelling and expression analysis of sea bass (Dicentrarchus labrax L.) interleukin-10". Paper presented in XXXIII Annual Meeting of the Sociedade Portuguesa de Imunologia, Braga, Portugal, 13th and 14th October 2006., 2006.
  42. do Vale, Ana; Costa-Ramos, Carolina; Silva, Alexandra; dos Santos, Nuno M.S.; Silva, Manuel T.. "Role of apoptosis induced by Photobacterium damselae subsp. piscicida in the ethiopathogenesis of acute fish pasteurellosis". Paper presented in EAFP 12th International Conference, Copenhagen, Denmark, 11th-16th September 2005., 2005.
  43. Nascimento, Diana S.; do Vale, Ana; Zou, Jun; Secombes, Christopher J.; dos Santos, Nuno M.S.. "Molecular characterisation and production of sea bass (Dicentrarchus labrax L.) recombinant tumour necrosis factor alpha (TNF-alpha)". Paper presented in EAFP 12th International Conference, Copenhagen, Denmark, 11th-16th September 2005., 2005.
  44. Reis, Marta I.R.; do Vale, Ana; Pinto, Cristina; dos Santos, Nuno M.S.. "Molecular cloning and characterization of sea bass (Dicentrarchus labrax L.) caspase-1, -3 and –9 genes". Paper presented in EAFP 12th International Conference, Copenhagen, Denmark, 11th-16th September 2005., 2005.
  45. do Vale, Ana; Afonso, António; Silva, Manuel T.. "The professional phagocytes of sea bass (Dicentrarchus labrax L.): cytochemical characterization of peritoneal neutrophils and macrophages". Paper presented in 10th International Conference of the EAFP, Dublin, Ireland, 9th-14th September 2001., 2001.
  46. dos Santos, Nuno M.S.; do Vale, Ana; Sousa, Maria J.; Silva, Manuel T.. "Mycobacterial infection in farmed turbot Scophthalmus maximus". Paper presented in 10th International Conference of the EAFP, Dublin, Ireland, 9th-14th September 2001., 2001.
  47. do Vale, Ana; Magariños, Beatriz; Lemos, Manuel C.; Toranzo, Alicia E.; Romalde, Jesus L.. "Utilization of haem compounds by Pasteurella piscicida". Paper presented in Third International Symposium on Aquatic Animal health. Baltimore. USA., 1998.
Journal article
  1. Lages, Marta A.; do Vale, Ana; Lemos, Manuel L.; Balado, Miguel. "Remodulation of bacterial transcriptome after acquisition of foreign DNA: the case of irp-HPI high-pathogenicity island in Vibrio anguillarum". mSphere (2023): http://dx.doi.org/10.1128/msphere.00596-23.
    10.1128/msphere.00596-23
  2. Johnny Lisboa; Cassilda Pereira; Rute D. Pinto; Inês Rodrigues; Liliana M. G. Pereira; Bruno Pinheiro; Pedro Oliveira; et al. "Unconventional structure and mechanisms for membrane interaction and translocation of the NF-kB-targeting toxin AIP56". Nature Communications (2023): http://dx.doi.org/10.1038/s41467-023-43054-z.
    10.1038/s41467-023-43054-z
  3. Ferreira, Inês A.; Peixoto, Diogo; Losada, Ana Paula; Quiroga, María Isabel; do Vale, Ana; Costas, Benjamín. "Early innate immune responses in European sea bass (Dicentrarchus labrax L.) following Tenacibaculum maritimum infection". Frontiers in Immunology 14 (2023): http://dx.doi.org/10.3389/fimmu.2023.1254677.
    10.3389/fimmu.2023.1254677
  4. Barca, Alba V.; Vences, Ana; Terceti, Mateus S.; do Vale, Ana; Osorio, Carlos R.. "Low salinity activates a virulence program in the generalist marine pathogen Photobacterium damselae subsp. damselae". mSystems 8 3 (2023): http://dx.doi.org/10.1128/msystems.01253-22.
    10.1128/msystems.01253-22
  5. Alexandra Teixeira; Inês Loureiro; Johnny Lisboa; Pedro N. Oliveira; Jorge E. Azevedo; Nuno M. S. dos Santos; Ana do Vale. "Characterization and Vaccine Potential of Outer Membrane Vesicles from Photobacterium damselae subsp. piscicida". International Journal of Molecular Sciences (2023): https://doi.org/10.3390/ijms24065138.
    10.3390/ijms24065138
  6. Inês Lua Freitas; Alexandra Teixeira; Inês Loureiro; Johnny Lisboa; Aurélia Saraiva; Nuno Miguel Simões dos Santos; Ana do Vale. "Susceptibility of Sea Bream (Sparus aurata) to AIP56, an AB-Type Toxin Secreted by Photobacterium damselae subsp. piscicida (Editor's Choice)". Toxins 14 2 (2022): 119-119. https://doi.org/10.3390/toxins14020119.
    10.3390/toxins14020119
  7. Xosé M. Matanza; Laura López-Suárez; Ana Vale; Carlos R. Osorio. "The two-component system RstAB regulates production of a polysaccharide capsule with a role in virulence in the marine pathogen Photobacterium damselae subsp. damselae". Environmental Microbiology (2021): https://doi.org/10.1111/1462-2920.15731.
    10.1111/1462-2920.15731
  8. Lisboa, Johnny; Pereira, Cassilda; Rifflet, Aline; Ayala, Juan; Terceti, Mateus S.; Barca, Alba V.; Rodrigues, Inês; et al. "A Secreted NlpC/P60 Endopeptidase from Photobacterium damselae subsp. piscicida Cleaves the Peptidoglycan of Potentially Competing Bacteria". mSphere 6 1 (2021): http://dx.doi.org/10.1128/msphere.00736-20.
    10.1128/msphere.00736-20
  9. Cassilda Pereira; Inês S. Rodrigues; Liliana M.G. Pereira; Johnny Lisboa; Rute D. Pinto; Leonor Araújo; Pedro Oliveira; et al. "Role of AIP56 disulphide bond and its reduction by cytosolic redox systems for efficient intoxication". Cellular Microbiology (2019): https://doi.org/10.1111/cmi.13109.
    10.1111/cmi.13109
  10. Abushattal, S.; Vences, A.; Dos Santos, N.M.S.; Do Vale, A.; Osorio, C.R.. "Draft genome sequences of photobacterium damselae subsp. Piscicida snw-8.1 and pp3, two fish-isolated strains containing a type III secretion system". Microbiology Resource Announcements 8 21 (2019): http://www.scopus.com/inward/record.url?eid=2-s2.0-85067227514&partnerID=MN8TOARS.
    10.1128/MRA.00426-19
  11. Terceti, M.S.; Vences, A.; Matanza, X.M.; Barca, A.V.; Noia, M.; Lisboa, J.; Dos Santos, N.M.S.; Do Vale, A.; Osorio, C.R.. "The RstAB system impacts virulence, motility, cell morphology, penicillin tolerance and production of type II secretion system-dependent factors in the fish and human pathogen photobacterium damselae subsp. damselae". Frontiers in Microbiology 10 APR (2019): http://www.scopus.com/inward/record.url?eid=2-s2.0-85068109857&partnerID=MN8TOARS.
    10.3389/fmicb.2019.00897
  12. Rodrigues, I.S.; Pereira, L.M.G.; Lisboa, J.; Pereira, C.; Oliveira, P.; dos Santos, N.M.S.; do Vale, A.. "Involvement of Hsp90 and cyclophilins in intoxication by AIP56, a metalloprotease toxin from Photobacterium damselae subsp. piscicida". Scientific Reports 9 1 (2019): http://www.scopus.com/inward/record.url?eid=2-s2.0-85067826636&partnerID=MN8TOARS.
    10.1038/s41598-019-45240-w
  13. Núñez-Díaz, José; Fumanal, Milena; do Vale, Ana; Fernández-Díaz, Catalina; Moriñigo, Miguel; Balebona, María. "Transcription of IVIAT and Virulence Genes in Photobacterium damselae subsp. piscicida Infecting Solea senegalensis". Microorganisms 6 3 (2018): 67. http://dx.doi.org/10.3390/microorganisms6030067.
    10.3390/microorganisms6030067
  14. do Vale, A.; Pereira, C.; Osorio, C.R.; dos Santos, N.M.S.. "The apoptogenic toxin aip56 is secreted by the type ii secretion system of photobacterium damselae subsp. Piscicida". Toxins 9 11 (2017): http://www.scopus.com/inward/record.url?eid=2-s2.0-85034449974&partnerID=MN8TOARS.
    10.3390/toxins9110368
  15. do Vale, A.; Cabanes, D.; Sousa, S.. "Bacterial toxins as pathogen weapons against phagocytes". Frontiers in Microbiology 7 FEB (2016): http://www.scopus.com/inward/record.url?eid=2-s2.0-84962082244&partnerID=MN8TOARS.
    10.3389/fmicb.2016.00042
  16. Pereira, L.M.G.; Pinto, R.D.; Silva, D.S.; Moreira, A.R.; Beitzinger, C.; Oliveira, P.; Sampaio, P.; et al. "Intracellular trafficking of AIP56, an NF-¿B-cleaving toxin from Photobacterium damselae subsp. piscicida". Infection and Immunity 82 12 (2014): 5270-5285. http://www.scopus.com/inward/record.url?eid=2-s2.0-84911433340&partnerID=MN8TOARS.
    10.1128/IAI.02623-14
  17. Silva, D.S.; Pereira, L.M.G.; Moreira, A.R.; Ferreira-da-Silva, F.; Brito, R.M.; Faria, T.Q.; Zornetta, I.; et al. "The Apoptogenic Toxin AIP56 Is a Metalloprotease A-B Toxin that Cleaves NF-¿b P65". PLoS Pathogens 9 2 (2013): http://www.scopus.com/inward/record.url?eid=2-s2.0-84880954175&partnerID=MN8TOARS.
    10.1371/journal.ppat.1003128
  18. Reis, M.I.R.; do Vale, A.; Pereira, P.J.B.; Azevedo, J.E.; dos Santos, N.M.S.. "Caspase-1 and IL-1ß Processing in a Teleost Fish". PLoS ONE 7 11 (2012): http://www.scopus.com/inward/record.url?eid=2-s2.0-84870595247&partnerID=MN8TOARS.
    10.1371/journal.pone.0050450
  19. Silva, A.; Almeida, B.; Sampaio-Marques, B.; Reis, M.I.R.; Ohlmeier, S.; Rodrigues, F.; Vale, A.D.; Ludovico, P.. "Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a specific substrate of yeast metacaspase". Biochimica et Biophysica Acta - Molecular Cell Research 1813 12 (2011): 2044-2049. http://www.scopus.com/inward/record.url?eid=2-s2.0-80054096814&partnerID=MN8TOARS.
    10.1016/j.bbamcr.2011.09.010
  20. Costa-Ramos, C.; Vale, A.D.; Ludovico, P.; dos Santos, N.M.S.; Silva, M.T.. "The bacterial exotoxin AIP56 induces fish macrophage and neutrophil apoptosis using mechanisms of the extrinsic and intrinsic pathways". Fish and Shellfish Immunology 30 1 (2011): 173-181. http://www.scopus.com/inward/record.url?eid=2-s2.0-78650697665&partnerID=MN8TOARS.
    10.1016/j.fsi.2010.10.007
  21. Silva, M.T.; dos Santos, N.M.S.; do Vale, A.. "AIP56: A novel bacterial apoptogenic toxin". Toxins 2 4 (2010): 905-918. http://www.scopus.com/inward/record.url?eid=2-s2.0-78649552139&partnerID=MN8TOARS.
    10.3390/toxins2040905
  22. Reis, M.I.R.; Costa-Ramos, C.; do Vale, A.; dos Santos, N.M.S.. "Molecular cloning of sea bass (Dicentrarchus labrax L.) caspase-8 gene and its involvement in Photobacterium damselae ssp. piscicida triggered apoptosis". Fish and Shellfish Immunology 29 1 (2010): 58-65. http://www.scopus.com/inward/record.url?eid=2-s2.0-77955057057&partnerID=MN8TOARS.
    10.1016/j.fsi.2010.02.016
  23. Silva, M.T.; Do Vale, A.; Dos Santos, N.M.N.. "Secondary necrosis in multicellular animals: An outcome of apoptosis with pathogenic implications". Apoptosis 13 4 (2008): 463-482. http://www.scopus.com/inward/record.url?eid=2-s2.0-41149115987&partnerID=MN8TOARS.
    10.1007/s10495-008-0187-8
  24. dos Santos, N.M.S.; do Vale, A.; Reis, M.I.R.; Silva, M.T.. "Fish and apoptosis: Molecules and pathways". Current Pharmaceutical Design 14 2 (2008): 148-169. http://www.scopus.com/inward/record.url?eid=2-s2.0-38949175453&partnerID=MN8TOARS.
    10.2174/138161208783378743
  25. Silva, M.T.; do Vale, A.; dos Santos, N.M.S.. "Fish and apoptosis: Studies in disease and pharmaceutical design". Current Pharmaceutical Design 14 2 (2008): 170-183. http://www.scopus.com/inward/record.url?eid=2-s2.0-38949102730&partnerID=MN8TOARS.
    10.2174/138161208783378734
  26. Nascimento, D.S.; Vale, A.d.; Tomás, A.M.; Zou, J.; Secombes, C.J.; dos Santos, N.M.S.. "Cloning, promoter analysis and expression in response to bacterial exposure of sea bass (Dicentrarchus labrax L.) interleukin-12 p40 and p35 subunits". Molecular Immunology 44 9 (2007): 2277-2291. http://www.scopus.com/inward/record.url?eid=2-s2.0-33846202982&partnerID=MN8TOARS.
    10.1016/j.molimm.2006.11.006
  27. Silva, D.S.P.; Reis, M.I.R.; Nascimento, D.S.; Vale, A.d.; Pereira, P.J.B.; dos Santos, N.M.S.. "Sea bass (Dicentrarchus labrax) invariant chain and class II major histocompatibility complex: Sequencing and structural analysis using 3D homology modelling". Molecular Immunology 44 15 (2007): 3758-3776. http://www.scopus.com/inward/record.url?eid=2-s2.0-34249286269&partnerID=MN8TOARS.
    10.1016/j.molimm.2007.03.025
  28. Reis, M.I.R.; Nascimento, D.S.; do Vale, A.; Silva, M.T.; dos Santos, N.M.S.. "Molecular cloning and characterisation of sea bass (Dicentrarchus labrax L.) caspase-3 gene". Molecular Immunology 44 5 (2007): 774-783. http://www.scopus.com/inward/record.url?eid=2-s2.0-33748785440&partnerID=MN8TOARS.
    10.1016/j.molimm.2006.04.028
  29. Nascimento, D.S.; Pereira, P.J.B.; Reis, M.I.R.; do Vale, A.; Zou, J.; Silva, M.T.; Secombes, C.J.; dos Santos, N.M.S.. "Molecular cloning and expression analysis of sea bass (Dicentrarchus labrax L.) tumor necrosis factor-a (TNF-a)". Fish and Shellfish Immunology 23 3 (2007): 701-710. http://www.scopus.com/inward/record.url?eid=2-s2.0-34447279908&partnerID=MN8TOARS.
    10.1016/j.fsi.2007.02.003
  30. Do Vale, A.; Costa-Ramos, C.; Silva, D.S.P.; Macedo, P.M.; Fernandes, R.; Sampaio, P.; Dos Santos, N.M.S.; Silva, M.T.. "Cytochemical and ultrastructural study of anoikis and secondary necrosis in enterocytes detached in vivo". Apoptosis 12 6 (2007): 1069-1083. http://www.scopus.com/inward/record.url?eid=2-s2.0-34247889986&partnerID=MN8TOARS.
    10.1007/s10495-006-0040-x
  31. do Vale, A.; Costa-Ramos, C.; Silva, A.; Silva, D.S.P.; Gärtner, F.; dos Santos, N.M.S.; Silva, M.T.. "Systemic macrophage and neutrophil destruction by secondary necrosis induced by a bacterial exotoxin in a Gram-negative septicaemia". Cellular Microbiology 9 4 (2007): 988-1003. http://www.scopus.com/inward/record.url?eid=2-s2.0-33947168734&partnerID=MN8TOARS.
    10.1111/j.1462-5822.2006.00846.x
  32. Pinto, R.D.; Nascimento, D.S.; Reis, M.I.R.; do Vale, A.; dos Santos, N.M.S.. "Molecular characterization, 3D modelling and expression analysis of sea bass (Dicentrarchus labrax L.) interleukin-10". Molecular Immunology 44 8 (2007): 2066-2075. http://www.scopus.com/inward/record.url?eid=2-s2.0-34147189842&partnerID=MN8TOARS.
    10.1016/j.molimm.2006.09.008
  33. Reis, M.I.R.; do Vale, A.; Pinto, C.; Nascimento, D.S.; Costa-Ramos, C.; Silva, D.S.P.; Silva, M.T.; dos Santos, N.M.S.. "First molecular cloning and characterisation of caspase-9 gene in fish and its involvement in a gram negative septicaemia". Molecular Immunology 44 7 (2007): 1765-1775. http://www.scopus.com/inward/record.url?eid=2-s2.0-33751258003&partnerID=MN8TOARS.
    10.1016/j.molimm.2006.07.301
  34. Pinto, R.D.; Nascimento, D.S.; Vale, A.D.; Santos, N.M.S.D.. "Molecular cloning and characterization of sea bass (Dicentrarchus labrax L.) CD8a". Veterinary Immunology and Immunopathology 110 1-2 (2006): 169-177. http://www.scopus.com/inward/record.url?eid=2-s2.0-32044440076&partnerID=MN8TOARS.
    10.1016/j.vetimm.2005.11.005
  35. Do Vale, A.; Silva, M.T.; Dos Santos, N.M.S.; Nascimento, D.S.; Reis-Rodrigues, P.; Costa-Ramos, C.; Ellis, A.E.; Azevedo, J.E.. "AIP56, a novel plasmid-encoded virulence factor of Photobacterium damselae subsp. piscicida with apoptogenic activity against sea bass macrophages and neutrophils". Molecular Microbiology 58 4 (2005): 1025-1038. http://www.scopus.com/inward/record.url?eid=2-s2.0-27944453406&partnerID=MN8TOARS.
    10.1111/j.1365-2958.2005.04893.x
  36. Do Vale, A.; Marques, F.; Silva, M.T.. "Apoptosis of sea bass (Dicentrarchus labrax L.) neutrophils and macrophages induced by experimental infection with Photobacterium damselae subsp. piscicida". Fish and Shellfish Immunology 15 2 (2003): 129-144. http://www.scopus.com/inward/record.url?eid=2-s2.0-0038639727&partnerID=MN8TOARS.
    10.1016/S1050-4648(02)00144-4
  37. Dos Santos, N.M.S.; Do Vale, A.; Sousa, M.J.; Silva, M.T.. "Mycobacterial infection in farmed turbot Scophthalmus maximus". Diseases of Aquatic Organisms 52 1 (2002): 87-91. http://www.scopus.com/inward/record.url?eid=2-s2.0-0037038713&partnerID=MN8TOARS.
  38. Do Vale, A.; Magariños, B.; Romalde, J.L.; Lemos, M.L.; Ellis, A.E.; Toranzo, A.E.. "Binding of haemin by the fish pathogen Photobacterium damselae subsp. piscicida". Diseases of Aquatic Organisms 48 2 (2002): 109-115. http://www.scopus.com/inward/record.url?eid=2-s2.0-0037061193&partnerID=MN8TOARS.
  39. Do Vale, A.; Afonso, A.; Silva, M.T.. "The professional phagocytes of sea bass (Dicentrarchus labrax L.): Cytochemical characterisation of neutrophils and macrophages in the normal and inflamed peritoneal cavity". Fish and Shellfish Immunology 13 3 (2002): 183-198. http://www.scopus.com/inward/record.url?eid=2-s2.0-0036712732&partnerID=MN8TOARS.
    10.1006/fsim.2001.0394
  40. Do Vale, A.; Ellis, A.E.; Silva, M.T.. "Electron microscopic evidence that expression of capsular polysaccharide by Photobacterium damselae subsp. piscicida is dependent on iron availability and growth phase". Diseases of Aquatic Organisms 44 3 (2001): 237-240. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035836424&partnerID=MN8TOARS.
  41. Ellis, A.E.; Do Vale, A.; Bowden, T.J.; Thompson, K.; Hastings, T.S.. "In vivo production of A-protein, lipopolysaccharide, iron-regulated outer membrane proteins and 70-kDa serine protease by Aeromonas salmonicida subsp. salmonicida". FEMS Microbiology Letters 149 2 (1997): 157-163. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030998110&partnerID=MN8TOARS.
    10.1016/S0378-1097(97)00063-3
Preprint
  1. Lisboa, Johnny; Pereira, Cassilda; Pinto, Rute D.; Rodrigues, Inês S.; Pereira, Liliana M. G.; Pinheiro, Bruno; Oliveira, Pedro; et al. "Structural and functional characterization of the NF-kB-targeting toxin AIP56 from Photobacterium damselae subsp. piscicida reveals a novel mechanism for membrane interaction and translocation". 2023. http://dx.doi.org/10.1101/2023.05.04.539443.
    10.1101/2023.05.04.539443
Thesis / Dissertation
  1. do Vale, Ana. "Anti-phagocytic mechanisms of Photobacterium damselae subsp. piscicida in sea bass pasteurellosis". PhD, Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, 2003.

Intellectual property

Patent
  1. do Vale, Ana; Nuno Miguel Simões dos Santos; Daniela Sá Pereira e Silva; Liliana Marisa Gonçalves Pereira; Jorge Eduardo Da Silva Azevedo; Pedro Pereira; Sandra MACEDO-RIBEIRO. 2014. "WO2014109658A3: AIP56 derived peptides (AIP56 as a biomedical and biotechnological tool for targeting NF-kB and for cargo-delivery)".
  2. do Vale, Ana; Nuno Miguel Simões dos Santos; Manuel Alexandre Teixeira da Silva; Jorge Eduardo Da Silva Azevedo. 2005. "WO2005014629A2: Protein from photobacterium damselae and use thereof".
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2023/09/11 The two-component system RstAB positively regulates the expression of secreted virulence factors in Photobacterium damselae subsp. piscicida 21st International Conference on Diseases of Fish and Shellfish (EAFP XXI), Aberdeen, Scotland, 11th-14th September 2023
EAFP-European Association of Fish Pathologists (Aberdeen, United Kingdom)
2023/09/11 Tenacibaculum maritimum can boost an inflammatory response in European seabass (Dicentrarchus labrax) upon peritoneal injection but cannot trigger tenacibaculosis disease 21st International Conference on Diseases of Fish and Shellfish (EAFP XXI), Aberdeen, Scotland, 11th-14th September 2023
EAFP-European Association of Fish Pathologists (Aberdeen, United Kingdom)
2023/03/17 PBT: a novel binary toxin from the fish pathogen Photobacterium damselae subsp. piscicida that targets fish, mouse and human macrophages (Best Oral Presentation Award) I ImmunoHUB Annual Meeting - Infection & Immunity
i3S - Instituto de Investigação e Inovação em Saúde (Porto, Portugal)
2022/05/04 The two-component system RstAB regulates expression of virulence factors in Photobacterium damselae subsp. piscicida IJUP - Encontro de Investigação Jovem
(Porto, Portugal)
2022/05/02 AIP56 and AIP56-like toxins: a family of bacterial toxins that target NF-kB 5th European NF-kappaB Subunit Workshop
(Porto, Portugal)
2022/04/26 Innate immune-related gene expression profiles in European sea bass (Dicentrarchus labrax) bath challenged with Tenacibaculum maritimum 21st Fish Immunology/Vaccination Workshop. Wageningen, Netherlands, 24-28th of April, 2022
(Wageningen, Netherlands)
2021/10/07 Differential local and systemic immune responses in European sea bass (Dicentrarchus labrax) following Tenacibaculum maritimum infection European Aquaculture Society Congress, Funchal, Portugal, 7th of October, 2021
(Funchal)
2021/09/21 Structural and functional characterization of a PirAB-like binary toxin from Photobacterium damselae subsp. piscicida EAFP 2021- 20th International Conference on Diseases of Fish and Shellfish
EAFP-European Association of Fish Pathologists (Online)
2021/09/20 Structural and functional characterization of NiTox: a new AB-toxin from Vibrio Nigripulchritudo lethal to shrimp Virtual 20th International Conference on Diseases of Fish and Shellfish
EAFP-European Association of Fish Pathologists (online)
2021/09/20 Innate immune-related gene expression profiles in European sea bass (Dicentrarchus labrax) bath challenged with Tenacibaculum maritimum Virtual 20th International Conference on Diseases of Fish and Shellfish
EAFP-European Association of Fish Pathologists (Online)
2021/09/20 Isolation and characterization of Outer Membrane Vesicles from Photobacterium damselae subsp. piscicida Virtual 20th International Conference on Diseases of Fish and Shellfish
EAFP-European Association of Fish Pathologists (online)
2021/09/17 Innate immune-related gene expression profiles in European sea bass (Dicentrarchus labrax) bath challenged with Tenacibaculum maritimum VIII Animal Science Workshop, ICBAS, Porto, Portugal
(Porto, Portugal)
2021/06/28 Photobacterium deadly duet: structure and activity of a novel virulence-associated binary toxin ETOX2021- European workshop on bacterial protein toxins
ETOX2021- European workshop on bacterial protein toxins (online, Germany)
2018/11/22 Proteins secreted by the type II secretion system of Photobacterium damselae subsp. piscicida: characterization of a novel NlpC/P60 containing peptidoglycan endopeptidase i3S Integrative Seminars
i3S- Instituto de Investigação e Inovação em Saúde (Porto, Portugal)
2017/06 The type II secretion of Photobacterium damselae piscicida mediates extracellular release of the apoptogenic toxin AIP56 18th European Workshop on Bacterial Protein Toxins, 26-30 June 2017, Paris, France.
Institute Pasteur (Paris, France)
2014/10 AIP56, a bacterial AB toxin targeting NF-kB i3S 4th Annual Meeting, 30-31 October 2014, Póvoa de Varzim, Portugal
i3s- Instituto de Investigação e Inovação em Saúde (Póvoa de Varzim, Portugal)
2009/09 Studies on AIP56, a key virulence factor of Photobacterium damselae subsp. piscicida 14th International Conference on Diseases of Fish and Shellfish, Prague, Czech Republic, September 14-18, 2009.
(Prague, Czech Republic)
2009/06 Structural and functional studies on AIP56, a novel bacterial apoptogenic toxin 14th European Workshop on Bacterial Protein Toxins, 27 June-02 Jully 2009, Obernai, France.
(Obernai, France)
1998/05 Caracterización de los mechanismos de utilización de compuestos hemo como fuentes de hierro por Pasteurella piscicida II Reunión Científica de Microbiología del Medio Acuático de la Sociedad Española de Microbiología, Girona, Spain, 29th- 31st May 1998.
(Girona, Spain)

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2021/01/01 - Current Characterization of a novel family of AB toxins with potential to be used as antigen delivery vehicles
Co-supervisor of Inês Lua Freitas
 Biologia Molecular e Celular (PhD)
Universidade do Porto, Portugal

Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal

Universidade do Porto Faculdade de Ciências, Portugal
2021/01/01 - Current Development of an antigen delivery platform based on AB toxins with specific tropism for APCs
Co-supervisor of Bruno Miguel da Silva Pinheiro
 Biologia Molecular e Celular (PhD)
Universidade do Porto, Portugal

Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal

Universidade do Porto Faculdade de Ciências, Portugal

Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2019/10/01 - Current Tenacibaculum maritimum pathogenesis: cross-talk between host and pathogen and beyond
Co-supervisor of Inês de Almeida Ferreira
 Ciência Animal (PhD)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2023/01/01 - 2023/07/21 Recombinant expression and purification of trimeric autotransporter adhesins from Photobacterium damselae subsp. piscicida
Supervisor of José Viveiros
 Bioquímica (Degree)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal

Universidade do Porto Faculdade de Ciências, Portugal

Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2021/09/15 - 2022/10/26 Studies on the regulation of Photobacterium damselae subsp. piscicida secreted proteins by the two-component system RstAB
Supervisor of Alexandre J Pinto
 Biochemistry (Master)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal

Universidade do Porto Faculdade de Ciências, Portugal
2021/07/15 - 2022/10/14 T3SS and T5SS in Photobacterium damselae subsp. piscicida: from basic studies to vaccine development
Supervisor of Inês Loureiro (Post-Doc)
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2021/07/15 - 2022/10/14 T3SS and T5SS in Photobacterium damselae subsp. piscicida: from basic studies to vaccine development
Supervisor of Johnny Lisboa (Post-Doc)
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2021/02/01 - 2022/09/05 Trimeric autotransporter adhesins (TAAs) from Photobacterium damselae subsp. piscicida: investigation of biological functions and recombinant production to use as antigens
Supervisor of Gabriela Alves Afonso
 Genética Molecular Comparativa e Tecnológica (Master)
Universidade de Trás-os-Montes e Alto Douro, Portugal

Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2019/01 - 2021/07/14 Identification and characterization of virulence factors secreted by Photobacterium damselae subsp. piscicida with potential to be used as antigens for vaccination against photobacteriosis
Supervisor of Inês Loureiro
 contracted post-doc (Other)
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2019/09 - 2020/07/15 Studies on the regulation of expression of AIP56 by RstA of Photobacterium damselae subsp. piscicida
Supervisor of Alexandre Pinto
 Bioquímica (Degree)
Universidade do Porto Faculdade de Ciências, Portugal

Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2016/10 - 2019/11 Host cell factors and molecular mechanisms implicated in intoxication by AIP56, a bacterial toxin targeting NF-kB
Supervisor of Cassilda Pereira
 post-doc fellowship (Other)
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2019/03 - 2019/07 Characterization of virulence factors secreted by Photobacterium damselae subsp. piscicida
Supervisor of Mateus de Souza Terceti
contracted post-doc (Other)
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2018/06 - 2018/11 Host cell factors and molecular mechanisms implicated in intoxication by AIP56, a bacterial toxin targeting NF-kB
Supervisor of Rita Pombinho
 post-doc fellowship (Other)
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2018/06 - 2018/10 Comparative analysis of proteins secreted by different strains of Photobacterium damselae subsp. piscicida
Supervisor
 Biotecnologia (Degree)
Instituto Politécnico de Coimbra Escola Superior Agrária de Coimbra, Portugal
2015/05 - 2015/09 Structural determinants required for AIP56 membrane translocation
Supervisor of Johnny Marques Lisboa
 post-doc fellowship (Other)
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2013/11 - 2015/05 Identification of structural determinants required for receptor binding and membrane translocation of AIP56, a Photobacterium damselae subsp. piscicida apoptogenic AB toxin targeting NF-kB
Supervisor of Rute Daniela Pinto
 post-doc fellowship (Other)
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2009/01/01 - 2015/02/23 Intracellular trafficking of AIP56, a bacterial AB toxin trageting NF-kB
Co-supervisor of Liliana Marisa Gonçalves Pereira
 Ciências Biomédicas (PhD)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2013/09/01 - 2014/11/16 Identification of the binding/translocation domain of AIP56
Supervisor of José Martinho Oliveira Peres
 Bioquímica (Master)
Universidade do Porto Faculdade de Ciências, Portugal
2013/09/01 - 2014/11/03 Biotechnological and clinical potential of AIP56 toxin
Co-supervisor of Inês Salvador Rodrigues
 Bioquímica (Master)
Universidade do Porto Faculdade de Ciências, Portugal
2013 - 2014 Expression of the protein quimera AIP56-IL3 using different expression vectors and solubility tags
Co-supervisor of Daniela Ferreira Garcia Rodrigues
 Bioquímica (Degree)
Universidade do Porto Faculdade de Ciências, Portugal

Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2013 - 2014 Clarification of structure-function relationships of AIP56, an apoptogenic bacterial toxin targeting NF-kB
Supervisor of José Pedro Quelhas
 Bioquímica (Degree)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal

Universidade do Porto Faculdade de Ciências, Portugal
2008/01/01 - 2013/07/16 Structural and functional characterization of AIP56, an apoptogenic exotoxin of Photobacterium damselae ssp. piscicida
Co-supervisor of Daniela Sá Pereira e Silva
 Ciências Biomédicas (PhD)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2008/01/01 - 2012/11/30 Molecular and genetic characterisation of sea bass caspases
Co-supervisor of Marta Inês Rocha Reis
 Ciências Biomédicas (PhD)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2012/04/01 - 2012/09/03 Optimization of immuno-electron microscopy and immunofluorescence protocols for studying AIP56 trafficking
Supervisor of Clara Isabel Moura Cabral
 Anatomia Patológica, Citológica e Tanatológica (Degree)
Instituto Politécnico do Porto Escola Superior de Saúde, Portugal
2010/10/01 - 2011/12/12 Development of a mammalian cell model to study AIP56
Supervisor of Silvia Liliana Gomes Mota
Biologia Aplicada (Master)
Universidade de Aveiro, Portugal
2007 - 2008 Studies on the pathological changes in the sea bass intestine associated to the pathogenesis and dissemination of Photobacterium damselae subsp. piscicida
Supervisor of Mariana Isabel da Cunha Monteiro Terra
 Bioquímica (Degree)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal

Universidade do Porto Faculdade de Ciências, Portugal
2004 - 2005 Contribution to the clarification of the mechanism of action of the AIP56 exotoxin from Photobacterium damselae subsp. piscicida
Supervisor of Ana Sofia Leite Silva de Magalhães
 Bioquímica (Degree)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal

Universidade do Porto Faculdade de Ciências, Portugal
2003 - 2004 Sequencing of sea bass (Dicentrarchus labrax) CD8 alpha
Supervisor
 Biologia (Degree)
Universidade do Porto Faculdade de Ciências, Portugal
2003 - 2004 Apoptosis and bacterial virulence: study of anti-phagocytic activity of Photobacterium damselae subsp. piscicida in pasteurellosis os Solea senegalensis
Supervisor of Joaquim Jorge Faria Ribeiro de Freitas
 Biologia Aplicada
Universidade do Minho, Portugal
2003 - 2004 Kinetics of activation of caspases in apoptotic peritoneal phagocytes from sea bass (Dicentrarchus labrax) injected with Photobacterium damselae subsp. piscicida (Phdp) concentrated supernatants and detection of apoptosis in tissues from sea bass with natural Phdp infection
Supervisor
 Biologia Aplicada (Degree)
Universidade do Minho, Portugal
2003 - 2004 Sea bass (Dicentrarchus labrax) caspase-9: partial sequencing and molecular characterization
Supervisor of Ana Cristina Prieto Machado Correia Pinto
 Biologia Aplicada (Degree)
Universidade do Minho, Portugal
2002 - 2003 Partial sequencing and molecular characterisation of sea bass (Dicentrarchus labrax) caspase-3
Supervisor
 Biologia Aplicada (Degree)
Universidade do Minho, Portugal

Event organisation

Event name
Type of event (Role) 		Institution / Organization
2013/06/26 - 2015/06/25 ETOX17- 17th European Workshop on Bacterial Protein Toxins; 20-24 June 2015. A meeting that brought together the most renowned world scientists, young researchers and students for exchanging and discussing the latest and most exciting findings regarding all aspects of the research within the field of bacterial protein toxins. (2015/06/20 - 2015/06/24)
Workshop (Member of the Organising Committee)
 Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal

Jury of academic degree

Topic
Role 		Candidate name (Type of degree)
Institution / Organization
2021/07/20 Transcriptomic study of Photobacterium damselae subsp. damselae: roles of temperature and of the two-component system RstAB as regulators of physiology and virulence
(Thesis) Main arguer
 Xosé Manuel Matanza Fente (PhD)
Universidade de Santiago de Compostela, Spain
2021/03/24 Characterisation of siderophores' receptors from Aeromonas salmonicida and their application in the design of new methods to control bacterial infections in aquaculture
(Thesis) Main arguer
 Diego Rey Varela (PhD)
Universidade de Santiago de Compostela, Spain
2021/03/16 Exploring the role of Vav3 in the repair of toxin-induced plasma membrane damage
(Thesis) Main arguer
 Ana Catarina Sampaio Ribeiro (Master)
Universidade do Minho Escola de Engenharia, Portugal
2018/09/10 Unravelling the role of keratins in bacterial infections
(Thesis) Arguer
 Rui Filipe da Silva e Cruz (PhD)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2018/07/05 Expression and purification of bacterial pore-forming toxins
(Thesis) Main arguer
 Patrícia Inês Notário Coelho (Other)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal

Universidade do Porto Faculdade de Ciências, Portugal
2018/06/29 Identification and characterization of virulence genes in Photobacterium damselae subsp. damselae, a pathogen of marine aquaculture
(Thesis) Main arguer
 Ana Vences Lorenzo (PhD)
Universidade de Santiago de Compostela, Spain
2017/12/21 Effects of dietary Amino acids supplementation on the European seabass (Dicentrarchus labrax) skin mucosal immune status
(Thesis) Main arguer
 Maria Carlota Pinto da Silva (Master)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2017/11/27 Application of experimental protocols for the study of the infection of Solea Senegalensis by Tenacibaculum maritimum
(Thesis) Main arguer
 Ildefonso José Vital Simões (Master)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2014/10/29 Molecular cytopathogenesis induced by the mycobacterium ulcerans toxin, mycolactone
(Thesis) Arguer
 José Bernardo da Costa Gama (PhD)
Universidade do Minho, Portugal
2013/11/29 Identification of novel non-muscle myosin IIA (NMIIA) interacting partners
(Thesis) Main arguer
 Marina Patrícia Fonseca Santos (Master)
Universidade do Porto Faculdade de Ciências, Portugal
2013/07/16 Structural and Functional Characterization of AIP56, An Apoptogenic Exotoxin of Photobacterium damselae subsp. piscicida
Supervisor
 Daniela de Sá Pereira e Silva (PhD)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2012/11/30 Molecular and genetic characterization of sea bass caspases
Supervisor
 Marta Inês Rocha Reis (PhD)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2012/10/12 Host-Pathogen interactions in Gram-positive bacteriosis in farmed turbot (Scophtalmus maximus)
(Thesis) Arguer
 Sónia Alexandra da Rocha Dias Gomes (PhD)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2010 NleC expression and purification
(Thesis) Main arguer
 António Simão Abreu (Other)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2010 Understanding Burulli Ulcer: Elucidation of the Cellular and Molecular Mechanisms Underlying Infection with Mycobacterium ulcerans
(Thesis) Arguer
 Alexandra Gabriel Fraga (PhD)
Universidade do Minho, Portugal
2008/10/24 Identification of bacteria from the genus Vibrio associated to the clam culture: characterization and pathogenesis
(Thesis) Main arguer
 Roxana Beaz Hidalgo (PhD)
Universidade de Santiago de Compostela, Spain

Ad Hoc journal article review

Journal title (ISSN) Publisher
2022/04/24 - Current Aquaculture
2022 - Current International Journal of Molecular Sciences (1422-0067) MDPI
2018/10/12 - Current Scientific Reports (2045-2322)
2016/11/30 - Current Frontiers in Cellular and Infection Microbiology (2235-2988)
2015/04/01 - Current Environmental Microbiology
2015/03/23 - Current Infection and Immunity
2014/04/22 - Current Research in Microbiology
2011/03/11 - Current Diseases of Aquatic Organisms
2006/06/08 - Current Fish & Shellfish Immunology
2006/05/18 - Current Veterinary Microbiology

Journal scientific committee

Journal title (ISSN) Publisher
2023 - Current Frontiers in Bacteriology (2813-6144) Frontiers Media, SA
2021/10/02 - Current Frontiers in Cell and Developmental Biology (2296-634X) Frontiers Media, SA
2021/08/03 - Current International Journal of Molecular Sciences (1422-0067) MDPI

Other jury / evaluation

Activity description Institution / Organization
2023/10/25 - Current Assessor in the probationary review of Manuel Correia, doctoral student of the Doctoral Program in Molecular and Cell Biology of the University of Porto. Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal