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Ana Isabel Oliveira Silva is graduated in Biology and Geology at the University of Minho, and holds a Master's degree in Applied Biochemistry, with specialization in Biomedicine, in the same University, with a final score of 18 (out of 20). During the last two years, she was a researcher at the Life and Health Sciences Research Institute (ICVS), University of Minho, in which she developed her master's thesis project (19 out of 20), entitled "Pre-clinical pharmacology of the monocarboxylate transporter 1 inhibitor AZD3965 in bladder cancer", supervised by Dr. Julieta Afonso and co-supervised by Dr. Ana Preto. This project aimed to evaluate the anticancer activity of AZD3965, a specific MCT1 inhibitor, in bladder cancer cell lines, by firstly comparing the metabolic differences between non-neoplastic ad neoplastic cell lines regarding protein and gene expression levels and lactate production. Then, she tested AZD3965 in muscle-invasive bladder cancer cell lines in vitro and in vivo, and combined AZD3965 with cisplatin . She acquired practice in several in vitro techniques (cell culture, RT-qPCR, immunofluorescence, Western Blot) and biological functional assays . Additionally, she acquired expertise in in vivo assays, as she used the CAM model for evaluating tumor growth and angiogenic capabilities, and used immunohistochemistry for proliferation and angiogenic markers evaluation. She was awarded a PhD scholarship in 2022 funded by FCT.
Identification

Personal identification

Full name
Ana Isabel Oliveira Silva
Gender
Female

Citation names

  • Silva, Ana
  • Silva, A

Author identifiers

Ciência ID
671B-E637-8F24
ORCID iD
0000-0001-5474-1566

Email addresses

  • anaisabelsilva2407@gmail.com (Professional)

Websites

Knowledge fields

  • Natural sciences - Biological Sciences - Biochemistry
  • Medical and Health Sciences
  • Medical and Health Sciences - Clinical Medicine - Oncology
  • Natural sciences - Biological Sciences - Cell Biology
  • Natural sciences - Biological Sciences - Molecular Biology

Languages

Language Speaking Reading Writing Listening Peer-review
English Proficiency (C2) Proficiency (C2) Advanced (C1) Proficiency (C2) Advanced (C1)
Portuguese (Mother tongue)
French Beginner (A1) Beginner (A1) Beginner (A1) Elementary (A2) Beginner (A1)
Spanish; Castilian Elementary (A2) Intermediate (B1) Beginner (A1) Upper intermediate (B2) Elementary (A2)
Education
Degree Classification
2022/09 - 2026
Ongoing
Health Sciences Doctoral Programme (Doutoramento)
Universidade do Minho, Portugal

Laboratório Associado ICVS 3B's, Portugal
2023/04/21
Concluded
Cell functional and phenotypical analysis: The usefulness of flow cytometry and related tools (Curso de doutoramento (conclusão de unidades curriculares))
Laboratório Associado ICVS 3B's, Portugal
2023/02/28
Concluded
Bioinformatics (Curso de doutoramento (conclusão de unidades curriculares))
Laboratório Associado ICVS 3B's, Portugal
2023/01/29
Concluded
Biostatistics (Curso de doutoramento (conclusão de unidades curriculares))
Laboratório Associado ICVS 3B's, Portugal
2022/12/15
Concluded
Laboratory Animal Science (Curso de doutoramento (conclusão de unidades curriculares))
Laboratório Associado ICVS 3B's, Portugal
2019/07/05 - 2022/03/10
Concluded
Bioquímica Aplicada (Mestrado)
Major in Biomedicina
Universidade do Minho, Portugal

Laboratório Associado ICVS 3B's, Portugal
"Pre-clinical pharmacology of the monocarboxylate transporter 1 inhibitor AZD3965 in bladder cancer" (THESIS/DISSERTATION)
Thesis: 19 out of 20 / Master's Degree: 18 out of 20
2020/06/01
Concluded
Advanced Course in Protein Degradation and Trafficking in Health and Sickness (Pós-Graduação)
Universidade do Minho, Portugal
17 out of 20
2020/06/01
Concluded
Advanced Course in Programmed Cell Death and Autophagy (Pós-Graduação)
Universidade do Minho, Portugal
18 out of 20
2020/06/01
Concluded
Advanced Course in Medicinal Chemistry II (Pós-Graduação)
Universidade do Minho, Portugal
16 out of 20
2015 - 2019/01/06
Concluded
Biologia e Geologia (Licenciatura)
Universidade do Minho, Portugal
14 out of 20
Affiliation

Science

Category
Host institution
Employer
2020/09/01 - Current Researcher (Research) Laboratório Associado ICVS 3B's, Portugal
Projects

Grant

Designation Funders
2022/09/01 - Current Novel chromene-based compounds as candidates for bladder cancer therapeutics
2022.11018.BD
PhD Student Fellow
Fundação para a Ciência e a Tecnologia
Ongoing
Outputs

Publications

Conference poster
  1. Silva, A; Félix, A; Cerqueira, M; Gonçalves, CS; Sampaio-Marques, B; Longatto-Filho, A; Baltazar, F; Afonso, J. "Effects of Lactate Transport Inhibition by AZD3965 in Muscle-Invasive Urothelial Bladder Cancer". Paper presented in III ASPIC/ASEICA International Meeting, 2023.
  2. Silva, A; Cerqueira, MC; Rosa,B; Sobral, C; Pinto-Ribeiro, F; Costa, MF; Baltazar, F; Afonso, J. "Prognostic Value of Monocarboxylate Transporter 1 Overexpression in Cancer: A Systematic Review". Paper presented in Porto Cancer Meeting, 2023.
  3. M Cequeira; E Belmonte-Reche; C Gonçalves; M Costa; R Rainha; A Silva; F Baltazar; J Gallo; M Bañobre. "Treatment of triple-negative breast cancer through externally triggered target-less drug carriers". Paper presented in Porto Cancer Meeting, 2023.
  4. Silva, Ana; Félix, Ana; Gonçalves, Céline; Baltazar, Fátima; Afonso, Julieta. "Pre-clinical pharmacology of the monocarboxylate transporter 1 inhibitor AZD3965 in bladder cancer". Paper presented in 4ª Encontro Nacional de Jovens Investigadores em Oncologia, 2022.
  5. "Preclinical pharmacology of the monocarboxylate transporter 1 inhibitor AZD3965 in bladder cancer". Paper presented in Porto Cancer Meeting, 2022.
Journal article
  1. Silva, Ana; Félix, Ana; Cerqueira, Mónica; Gonçalves, Céline S.; Sampaio-Marques, Belém; Longatto-Filho, Adhemar; Baltazar, Fátima; Afonso, Julieta. "Effects of Lactate Transport Inhibition by AZD3965 in Muscle-Invasive Urothelial Bladder Cancer". Pharmaceutics 15 12 (2023): 2688. http://dx.doi.org/10.3390/pharmaceutics15122688.
    10.3390/pharmaceutics15122688
  2. Silva, Ana; Cerqueira, Mónica Costa; Rosa, Beatriz; Sobral, Catarina; Pinto-Ribeiro, Filipa; Costa, Marta Freitas; Baltazar, Fátima; Afonso, Julieta. "Prognostic Value of Monocarboxylate Transporter 1 Overexpression in Cancer: A Systematic Review". International Journal of Molecular Sciences 24 6 (2023): 5141. http://dx.doi.org/10.3390/ijms24065141.
    10.3390/ijms24065141
  3. Silva, Ana; Antunes, Beatriz; Batista, Alberta; Pinto-Ribeiro, Filipa; Baltazar, Fátima; Afonso, Julieta. "In Vivo Anticancer Activity of AZD3965: A Systematic Review". Molecules 27 1 (2021): 181. http://dx.doi.org/10.3390/molecules27010181.
    Open access • Published • 10.3390/molecules27010181
Activities

Event participation

Activity description
Type of event
Event name
Institution / Organization
2022/09/24 - Current 4º Encontro Nacional de Jovens Investigadores em Oncologia
Meeting
4ª Encontro Nacional de Jovens Investigadores em Oncologia
Liga Portuguesa Contra o Cancro Núcleo Regional do Norte, Portugal
2022/05/12 - 2022/05/13 Porto Cancer Meeting
Universidade do Porto, Portugal
2021/01/29 - 2021/01/29 II Simpósio Bioquímica Aplicada
Universidade do Minho, Portugal
2019/09/27 - 2019/09/27 Noite Europeia dos Investigadores Noite Europeia dos Investigadores
Universidade do Minho, Portugal

Universidade de Lisboa, Portugal

Universidade de Évora, Portugal

Course / Discipline taught

Academic session Degree Subject (Type) Institution / Organization
2021/09/01 - Current Natural Sciences / Physics and Chemistry / Mathematics / English (Ensino básico 3º ciclo) Ser e Saber+, Portugal