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Augusto Q. H. Pedro graduated in Biochemistry in 2009 by University of Beira Interior (UBI), and during 2009 he participated as a volunteer researcher in a project concerning the purification of pegylated proteins at (CICS-UBI). He finished his MSc in Biochemistry in 2011 (UBI, Portugal) wherein he carried out research at Health Sciences Research Centre (CICS-UBI), and was also an intern at Technical University of Lodz, Poland, under the supervision of Prof. Stanislaw Wysocki. He was then granted with a PhD fellowship from FCT (SFRH/BD/81222/2011), and by the beginning of 2016 he completed his PhD in Biochemistry by CICS-UBI, marked as excellent. From 2016 to january 2019, he was a Post-doctoral researcher at University of Aveiro, (CICECO ¿ Aveiro Institute of Materials, Chemistry department), hired as member of the research team of an European Research Council (ERC)-funded project, namely IgY Technology: A Purification Platform using Ionic-Liquid-Based Aqueous Biphasic Systems (IgYPurTech). Augusto had then several roles as a young researcher at CICECO, initially hired under the scope of FCT project IF/01634/2015 as then as a first level doctorate researcher within the scope of FCT project PTDC/BII-BBF/030840/2017. Currently, he is a Junior Researcher at University of Aveiro, hired under the Scientific Employment Stimulus ¿ Individual Call from FCT, undertaken under a very competitive basis (approval rate circa 8%). He is/was the Principal Investigator (PI) of the Projects IL2BioPro (FCT, 214367€), mVACCIL (FCT, 49949.48€, overall approval rate of 8.7%) and co-PI of PureDNA (FCT, 49903€). In the last years, he has been using ionic liquids as alternative solvents to improve the stability of biopharmaceuticals and as integrated stabilization/purification platforms in different formats. Since 2011, he (co)authored 31 scientific articles (2 as corresponding author) in international peer-reviewed journals, 1 in a national Journal and 1 white paper, 3 chapter in highly reputed journals in the fields of sustainable chemistry and (applied) biotechnology. Recently, Augusto acted as Chief Guest Editor of a special issue from Life (MDPI). He is/was supervisor of 2PhD students (Funded by FCT), 2 research fellows, 6 MSc theses and 8 BSc final projects (University of Beira Interior and University of Aveiro). Aiming to disseminate the knowledge he has acquired, Augusto has participated in several national and international conferences and has lectured practical classes at University of Beira Interior (Covilhã, Portugal) and University of Aveiro (Aveiro, Portugal). To protect IP rights of the outcomes of the research projects he has participated, Augusto has filled 3 patents, and has been developing his skills in entrepreneurship and technology commercialization strategies by the participation in several programmes (Hitech, Born from Knowledge). Overall, Augusto has multidisciplinary skills and an outstanding commitment to science in benefit of environment and society.
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Identification

Personal identification

Full name
Augusto Quaresma Henriques Pedro

Citation names

  • Augusto Pedro
  • Augusto Q. Pedro
  • A. Q. Pedro

Author identifiers

Ciência ID
7111-B2BE-336D
ORCID iD
0000-0001-8194-6022
Google Scholar ID
https://scholar.google.com/citations?user=oer44UoAAAAJ&hl=pt-PT&oi=ao
Scopus Author Id
54080426100

Websites

Knowledge fields

  • Natural sciences - Biological Sciences - Molecular Biology
  • Engineering and Technology - Industrial Biotechnology - Fermentation
  • Exact Sciences - Chemical Sciences - Analytical Chemistry
  • Engineering and Technology - Industrial Biotechnology - Industrial Biotechnology
  • Engineering and Technology - Industrial Biotechnology - Industrial Biotechnology
  • Engineering and Technology - Industrial Biotechnology - Industrial Biotechnology

Languages

Language Speaking Reading Writing Listening Peer-review
English Advanced (C1) Proficiency (C2) Advanced (C1) Proficiency (C2) Upper intermediate (B2)
Spanish; Castilian Elementary (A2) Upper intermediate (B2) Beginner (A1) Intermediate (B1) Elementary (A2)
Portuguese (Mother tongue)
Education
Degree Classification
2016
Concluded
 Biochemistry (Doutoramento)
Universidade da Beira Interior Faculdade de Ciências, Portugal
"Biosynthesis, isolation and kinetic characterization of recombinant human catechol-O-methyltransferase from Pichia pastoris strains " (THESIS/DISSERTATION)
 Excellent
2011
Concluded
 Biochemistry (Mestrado)
Major in Industrial Biotechnology
Universidade da Beira Interior Faculdade de Ciências, Portugal
"Biosynthesis, solubilization and purification of human membrane bound Catechol-O-Methyltransferase in Brevibacillus choshinensis cells " (THESIS/DISSERTATION)
 18
2009
Concluded
 Biochemistry (Licenciatura)
Universidade da Beira Interior Faculdade de Ciências, Portugal
""Tryptase use as a diagnostic marker in patients with monoclonal gammophaties."" (THESIS/DISSERTATION)
 14
Affiliation

Science

Category
Host institution 		Employer
2019/05 - Current Contracted Researcher (Research) Universidade de Aveiro, Portugal
Universidade de Aveiro CICECO, Portugal
2019/06/22 - 2019/07/05 Visiting Researcher (Research) Centre de Biophysique Moléculaire, France
Centre de Biophysique Moléculaire, France
(...)
2019/02 - 2019/05 Postdoc (Research) Universidade de Aveiro CICECO, Portugal
Universidade de Aveiro CICECO, Portugal
2016/09 - 2019/02 Postdoc (Research) Universidade de Aveiro CICECO, Portugal
Universidade de Aveiro CICECO, Portugal
2012/01/01 - 2016/02/29 Researcher (Research) Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2010/09 - 2011/06 Researcher (Research) Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2010/07/01 - 2010/08/31 Visiting Researcher (Research) Politechnika Lodzka Instytut Chemii Ogólnej i Ekologicznej, Poland
Politechnika Lodzka Instytut Chemii Ogólnej i Ekologicznej, Poland

Teaching in Higher Education

Category
Host institution 		Employer
2021/02 - Current Invited Assistant (University Teacher) Universidade de Aveiro, Portugal
Universidade de Aveiro Departamento de Química, Portugal
2012/09/01 - 2016/02/01 Invited Assistant (University Teacher) Universidade da Beira Interior, Portugal
Universidade da Beira Interior, Portugal

Others

Category
Host institution 		Employer
2022/01/17 - 2024/01/16 mVACCIL - Improving the manufacturing of mRNA vaccines applying ionic liquids and integrated production-clarification processes - EXPL/BII-BTI/0731/2021. FCT. Principal Investigator Fundação para a Ciência e a Tecnologia, Portugal
Universidade de Aveiro CICECO, Portugal
2018/07/01 - 2022/06/30 IL2BioPro - Towards the improvement of recombinant proteins bioprocessing using ionic liquids. FCT, Portuguese Foundation for Science and Technology, POCI-01-0145-FEDER-030840. Principal Investigator Fundação para a Ciência e a Tecnologia, Portugal
Universidade de Aveiro CICECO, Portugal
2014 - 2016 Responsible of the Centrifuge Laboratory Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2009/01/01 - 2009/12/13 Participation as a volunteer in a scientific project about the separation and purification of pegylated proteins, under the supervision of Professor Ana Cristina Dias-Cabral. Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
Projects

Grant

Designation Funders
2019/02 - 2019/05 Sustainable purification platforms for RNA
FCT IF/01634/2015
Post-doc Fellow
Universidade de Aveiro CICECO, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2016/09 - 2019/01 IgY Technology: A Purification Platform using Ionic-Liquid-Based Aqueous Biphasic Systems (IgYPurTech)
2013-StG-337753
Post-doc Fellow
Universidade de Aveiro CICECO, Portugal
 European Research Council
Concluded
2012/01/01 - 2016/12/31 Biosynthesis, isolation and kinetic characterization of recombinant human catechol-O-methyltransferase isolated from Pichia pastoris
SFRH/BD/81222/2011
PhD Student Fellow
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded

Contract

Designation Funders
2021 - Current Cost-effective and sustainable purification platforms for protein-based biopharmaceuticals - 2020.02599.CEECIND
2020.02599.CEECIND
Researcher
Universidade de Aveiro, Portugal

Universidade de Aveiro Departamento de Química, Portugal

Universidade de Aveiro CICECO, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2019/05 - 2021 IL2BioPro: Towards the improvement of recombinant proteins bioprocessing using ionic liquids
PTDC/BII-BBF/030840/2017
Researcher
Universidade de Aveiro, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing

Other

Designation Funders
2022/03 - Current Yscript: Yeast cell factory for mRNA bioproduction
101047214
Researcher
Universidade de Aveiro CICECO, Portugal
 European Innovation Council
Ongoing
2023/01 - 2025/12 PRR - Recovery and Resilience Plan Agenda for Business Innovation InsectERA
C644917393-00000032
Researcher
Universidade de Aveiro CICECO, Portugal
Ongoing
2023/01/01 - 2024/06/30 PureDNA: Development of cost-effective platforms based on ionic liquids for the purification of p53-minicircle DNA biopharmaceuticals with application in oncology
2022.03394.PTDC
Co-Principal Investigator (Co-PI)
Universidade de Aveiro CICECO, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2022/01/17 - 2024/01/16 mVACCIL - Improving the manufacturing of mRNA vaccines applying ionic liquids and integrated production-clarification processes (EXPL/BII-BTI/0731/2021, FCT)
EXPL/BII-BTI/0731/2021
Principal investigator
Universidade de Aveiro, Portugal

Universidade de Aveiro CICECO, Portugal

Universidade de Aveiro Unidade de Investigação em Governança Competitividade e Políticas Públicas, Portugal

Universidade de Aveiro Centro de Estudos do Ambiente e do Mar, Portugal

Universidade de Aveiro Instituto de Biomedicina, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2018/07 - 2022/06/30 IL2BioPro: Towards the improvement of recombinant proteins bioprocessing using ionic liquids
PTDC/BII-BBF/030840/2017
Principal investigator
Universidade de Aveiro CICECO, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2018 - 2022 PUREmirSils: Ionic liquid-based supports for pre-miRNAs purification targeting Alzheimers disease
POCI-01-0145-FEDER-029496
Researcher
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal

Universidade de Aveiro CICECO, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2018 - 2019/12 Pre-miRNAs for Alzheimers disease therapy: biosynthesis, purification and delivery
Acordo bilateral FCT-PHC (Programa Pessoa)
Researcher
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2012 - 2014 Biosynthesis, purification and biointeraction of human soluble catechol-O-methyltransferase with Parkinson´s inhibitors
Provided by PTCRIS: 128284
128284
EXPL/BBB478/BQB/0960/2012
Researcher
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
 Fundação para a Ciência e a Tecnologia, I.P.
Concluded
Outputs

Publications

Book chapter
  1. Augusto Pedro. "Ionogels for Biomedical Applications". 391-425. Springer Singapore, 2022.
    10.1007/978-981-16-7483-9_18
  2. Inês S. Cardoso; Augusto Q. Pedro (7111-B2BE-336D); Armando J. D. Silvestre; Mara G. Freire. "Ionic Liquids and Deep Eutectic Solvents in the Field of Environmental Monitoring". In Green Chemistry and Sustainable Technology, edited by Justyna Plotka-Wasylka; Jacek Namiesnik, 203-240. Singapore: Springer Singapore, 2019.
    Published • 10.1007/978-981-13-9105-7_8
  3. Augusto Q. Pedro (7111-B2BE-336D); João A. P. Coutinho; Mara G. Freire. "Immobilization of Ionic Liquids, Types of Materials, and Applications". In Encyclopedia of Ionic Liquids, edited by Suojiang Zhang, 1-12. Singapore: Springer Singapore, 2019.
    Published • 10.1007/978-981-10-6739-6_86-1
Conference abstract
  1. Luís António Paulino Passarinha; Filipa Correia; Augusto Q. Pedro; Diana Oliveira; João António de Sampaio Rodrigues Queiroz. "Biosynthesis, purification and biointeraction of human SCOMT with Parkinson's disease inhibitors". Paper presented in 16th European Congress on Biotechnology, New Biotechnology, Edinburgh, 2014.
    Published • 10.1016/j.nbt.2014.05.942
Conference poster
  1. L. S. Castro, P. Pereira, F. Sousa, J. A. P. Coutinho, A. P. M. Tavares, M. G. Freire, A. Q. Pedro. "Improvement of recombinant proteins purification by the use of ionic liquids as adjuvants in aqueous two-phase systems". Paper presented in MicroBiotec’19 – Congress of Microbiology and Biotechnology. Coimbra, Portugal, 2019.
  2. M. J. Quental, A. Q. Pedro, P. Pereira, M. Sharma, J. A. Queiroz, J. A. P. Coutinho, F. Sousa, M. G. Freire. "Bio-based ionic liquids as integrated extraction-preservation strategies in RNA bioprocessing". Paper presented in ILSEPT – 4th International Conference on Ionic Liquids in Separation and Purification Tehcnology, Sitges, Spain, 2019.
  3. M. C. Neves, P. Pereira, A. Q. Pedro, J. C. Martins, T. Trindade, J. A. Queiroz, M. G. Freire, F. Sousa. "Isolation of nucleic acids using novel ionic-liquid-modified stationary phases". Paper presented in ILSEPT – 4th International Conference on Ionic Liquids in Separation and Purification Tehcnology, Sitges, Spain, 2019.
  4. A. Q. Pedro, P. Pereira, M. J. Quental, A. P. Carvalho, S. M. Santos, J. A. Queiroz, F. Sousa, M. G. Freire. "Stabilization of recombinant RNA using biological-based ionic liquids". Paper presented in ILSEPT – 4th International Conference on Ionic Liquids in Separation and Purification Tehcnology, Sitges, Spain, 2019.
  5. T. Santos, P. Pereira, A. Q. Pedro, J. Dias, A. Viegas, E. J. Cabrita, C. Cruz. "G-quadruplex recognition by the nucleolin protein in prostate cancer cells". Paper presented in PTNMR Meeting, SMASH – Small Molecule NMR Conference, Porto, Portugal, 2019.
  6. R. Carapito, M. Neves, P. Pereira, A. Q. Pedro, J. A. Queiroz, M. G. Freire, F. Sousa. "Ionic liquids-based supports for the separation of nucleic acids". Paper presented in III International Congress in Health Sciences Research: Towards Innovation and Entrepreneurship. Covilhã, Portugal, 2019.
  7. T. Santos, P. Pereira, A. Q. Pedro, J. S. Dias, A. Viegas, J. A. Queiroz, E. J. Cabrita, C. Cruz. "Nucleolin/miR-149 complex: A strategy in the target of prostate cancer cells". Paper presented in III International Congress in Health Sciences Research: Towards Innovation and Entrepreneurship. Covilhã, Portugal, 2019.
  8. L. S. Castro, M. R. Almeida, P. Pereira, F. Sousa, J. A. P. Coutinho, A. P. M. Tavares, M. G. Freire, A. Q. Pedro. "Aqueous two-phase systems comprising ionic liquids for the extraction of recombinant proteins". Paper presented in XIV Annual CICS-UBI Symposium (Universidade da Beira Interior), Covilhã, Portugal, 2019.
  9. Márcia C. Neves, Patrícia Pereira, Augusto Q. Pedro, João C. Martins, João A. Queiroz, Mara G. Freire, Fani Sousa. "Preparative chromatography of recombinant nucleic acids using ionic liquids as multimodal ligands". Paper presented in National Congress of Biotechnology (BIOTEC), Vigo, Spain, 2019.
  10. L. S. Castro, M. R. Almeida, P. Pereira, F. Sousa, J. A. P. Coutinho, A. P. M. Tavares, A. Q. Pedro, M. G. Freire. "Improving the purification of recombinant proteins by the use of ionic liquids as adjuvants in aqueous two-phase systems". Paper presented in Jornadas CICECO 2019 – Where Science Meets Society (Universidade de Aveiro), Aveiro, Portugal, 2019.
  11. Maria V. Quental, Augusto Q. Pedro, Patrícia Pereira, Mukesh Sharma, João A. P. Coutinho, Fani Sousa, M. G. Freire. "Integrated extraction-preservation strategies for RNA bioprocessing using biobased ionic liquids". Paper presented in Jornadas CICECO 2019 – Where Science Meets Society (Universidade de Aveiro), Aveiro, Portugal, 2019.
  12. MJ Quental, AQ Pedro, P Pereira, M Sharma, Queiroz JA, Coutinho JAP, Sousa F, Freire MG. "Bio-based ionic liquids as extraction-preservation strategies in RNA bioprocessing". Paper presented in ISGC 2019, La Rochelle, France, 2019.
  13. P Pereira, MC Neves, AQ Pedro, I Rodrigues, JC Martins, T Trindade, JA Queiroz, MG Freire, F Sousa. "Separation of nucleic acids using ionic-liquid functionalized macroporous supports". Paper presented in ChemPor 2018 – 13th International Chemical and Biological Engineering Conference, Aveiro, Portugal, 2018.
  14. AQ Pedro, P. Pereira, MJ Quental, AP Carvalho, SM Santos, JA Queiroz, F Sousa, MG Freire. "Biological-based ionic liquids as novel preservation media for recombinant RNA". Paper presented in ChemPor 2018 – 13th International Chemical and Biological Engineering Conference, Aveiro, Portugal, 2018.
  15. Azevedo GM, Valente JFA, Sousa Â, Pedro AQ, Pereira P, Queiroz JA, Sousa F. "Effect of the purification conditions on the p53-pDNA stability and biological activity”". Paper presented in XIII Annual CICS-UBI symposium, Covilhã, Portugal, 2018.
  16. Pedro AQ, Antunes JA, Pereira P, Passarinha LA, Sousa F, Coutinho JAP, Freire MG. "Bioprocessing of interferon alfa 2b using ionic-liquid-based systems". Paper presented in BPP 2017 – Biopartitioning and Purification Conference, Copenhagen, Denmark, 2017.
  17. Barreira M, Pereira P, Pedro AQ, Figueiras R, Sousa F. "Ionic-liquid assisted-purification of pre-miR-29b for delivery in lung cancer cells". Paper presented in XII Annual CICS-UBI Symposium. Covilhã, Portugal, 2017.
  18. Barreira M, Pereira P, Pedro AQ, Freire MG, Figueiras AR, Sousa F. "Pre-miR-29 as a lung cancer therapeutic: improving purity with ionic liquids and delivery with functionalized nanosystems". Paper presented in II International Congress in Health Sciences Research: Towards Innovation and Entrepreneurship. Covilhã, Portugal, 2017.
  19. Duarte DR, Pedro AQ, Maia CJ, Passarinha LA. "Improvement of STEAP1 biosynthesis from Pichia pastoris X33 cells under an optimized feeding strategy". Paper presented in II International Congress in Health Sciences Research: Towards Innovation and Entrepreneurship. Covilhã, Portugal, 2017.
  20. Augusto Pedro, Joana Antunes, Patrícia Pereira, Luís Passarinha, João Coutinho, Mara Freire. "The role of biocompatible ionic liquids in the purification and stabilization of recombinant interferon alpha 2b". Paper presented in 9th Conference on Recombinant Protein Production (RPP9). Dubrovnik, Croatia, 2017.
  21. Pereira P, Pedro AQ, Tomás J, Sousa A, Queiroz JA, Figueiras R, Sousa F. "Intra and Extracellular Pre-miR-29b Purification by an Agmatine monolithic support". Paper presented in 9º Encontro Nacional de Cromatografia & XVI COLACRO. Lisboa, Portugal, 2015.
  22. Pedro AQ, Bonifácio MJ, Queiroz JA, Passarinha LA. "Purification of histidine-tagged human membrane-bound catechol-O-methyltransferase". Paper presented in 9º Encontro Nacional de Cromatografia & XVI COLACRO. Lisboa, Portugal, 2015.
  23. Pereira P, Pedro AQ, Tomás J, Sousa Â, Queiroz JA, Figueiras A, Sousa F. "Polyplexes-mediated delivery of pre-miR-29b for Alzheimer’s disease therapeutics". Paper presented in I International Congress in Health Sciences Research - Towards Innovation and Entrepreneurship: Trends in Endocrinology and Neurosciences. Covilhã, Portugal, 2015.
  24. Pedro AQ, Martins LM, Dias JML, Bonifácio MJ, Queiroz JA, Passarinha, LA. "An artificial neural networks for membrane-bound catechol-O-methyltransferase biosynthesis in mini-bioreactors with Pichia pastoris methanol-induced cultures networks". Paper presented in 8th Conference on Recombinant Protein Production (RPP). Mallorca, Spain, 2015.
  25. Pedro AQ, Martins LM, Dias JML, Bonifácio MJ, Queiroz JA, Passarinha, LA. "Membrane-bound catechol-O-methyltransferase biosynthesis in Pichia pastoris: optimization of the induction phase using artificial neural networks". Paper presented in Protein & Antibody Engineering Summit (PEGS) Europe, Lisboa, Portugal, 2014.
  26. Correia FF, Pedro AQ, Oliveira D, Queiroz JA, Passarinha LA. "Biosynthesis, Purification and Biointeraction of human SCOMT with Parkinson’s disease inhibitors". Paper presented in 16th European Congress on Biotechnology (ECB). Edinburgh, Scotland, 2014.
  27. Pedro AQ, Oppolzer D, Bonifácio MJ, Maia CJ, Queiroz JA, Passarinha LA. "Evaluation of MutS and Mut+ Pichia pastoris strains for membrane-bound COMT biosynthesis". Paper presented in 13' Microbiotec - Portuguese Congress of Microbiology and Biotechnology, 2013.
  28. Pedro AQ, Oppolzer D, Bonifácio MJ, Maia CJ, Queiroz JA, Passarinha, LA. "Evaluation of a MutS and Mut+ Pichia pastoris strains for membrane-bound COMT biosynthesis". Paper presented in Modern biophysical methods for protein-ligand interactions – EMBO practical course, 2013.
  29. Soares R, Ferreira S, Pedro AQ, Gonçalves AM, Bonifácio MJ, Queiroz JA, Maia CJ, Passarinha LA. "Biosynthesis of human membrane-bound catechol-O-methyltransferase optimization using Plackett-Burman central composite rotatable design". Paper presented in 9th European Symposium on Biochemical Engineering Science (ESBES). Istanbul, Turkey, 2012.
  30. Pedro AQ, Bonifácio MJ, Queiroz JA, Maia CJ, Passarinha LA. "Strategies to improve recombinant human membrane protein overexpression for structural, functional and clinical studies". Paper presented in Jornadas sobre Tecnologia e Saúde. Guarda, Portugal, 2012.
Journal article
  1. Almeida, Catarina; Pedro, Augusto Q.; Tavares, Ana P. M.; Neves, Márcia C.; Freire, Mara G.. "Ionic-liquid-based approaches to improve biopharmaceuticals downstream processing and formulation". Frontiers in Bioengineering and Biotechnology 11 (2023): http://dx.doi.org/10.3389/fbioe.2023.1037436.
    10.3389/fbioe.2023.1037436
  2. Capela, Emanuel V.; Bairos, Jéssica; Pedro, Augusto Q.; Neves, Márcia C.; Raquel Aires-Barros, M.; Azevedo, Ana M.; Coutinho, João A.P.; Tavares, Ana P.M.; Freire, Mara G.. "Supported ionic liquids as customizable materials to purify immunoglobulin G". Separation and Purification Technology 305 (2023): 122464. http://dx.doi.org/10.1016/j.seppur.2022.122464.
    10.1016/j.seppur.2022.122464
  3. Gonçalves, Ana M.; Pedro, Augusto Q.; Oliveira, Diana M.; Oliveira, Adriana E.; Santos, Marino F. A.; Correia, Márcia A. S.; Queiroz, João A.; et al. "Thermofluor-Based Optimization Strategy for the Stabilization of Recombinant Human Soluble Catechol-O-Methyltransferase". International Journal of Molecular Sciences 23 20 (2022): 12298. http://dx.doi.org/10.3390/ijms232012298.
    10.3390/ijms232012298
  4. Gonçalves, A.M.; Sousa, Â.; Pedro, A.Q.; Romão, M.J.; Queiroz, J.A.; Gallardo, E.; Passarinha, L.A.. "Advances in Membrane-Bound Catechol-O-Methyltransferase Stability Achieved Using a New Ionic Liquid-Based Storage Formulation". International Journal of Molecular Sciences 23 13 (2022): http://www.scopus.com/inward/record.url?eid=2-s2.0-85133120009&partnerID=MN8TOARS.
    10.3390/ijms23137264
  5. Pereira, Patrícia; Pedro, Augusto Q.; Neves, Márcia C.; Martins, João C.; Rodrigues, Inês; Freire, Mara G.; Sousa, Fani. "Efficient Isolation of Bacterial RNAs Using Silica-Based Materials Modified with Ionic Liquids". Life 11 10 (2021): 1090. http://dx.doi.org/10.3390/life11101090.
    Published • 10.3390/life11101090
  6. Duarte, D. R.; Barroca-Ferreira, J.; Gonçalves, A. M.; Santos, F. M.; Rocha, S. M.; Pedro, A. Q.; Maia, C. J.; Passarinha, L. A.. "Impact of glycerol feeding profiles on STEAP1 biosynthesis by Komagataella pastoris using a methanol-inducible promoter". Applied Microbiology and Biotechnology 105 11 (2021): 4635-4648. http://dx.doi.org/10.1007/s00253-021-11367-y.
    Published • 10.1007/s00253-021-11367-y
  7. Castro, Leonor S.; Lobo, Guilherme S.; Pereira, Patrícia; Freire, Mara G.; Neves, Márcia C.; Pedro, Augusto Q.. "Interferon-Based Biopharmaceuticals: Overview on the Production, Purification, and Formulation". Vaccines 9 4 (2021): 328. http://dx.doi.org/10.3390/vaccines9040328.
    Open access • Accepted • 10.3390/vaccines9040328
  8. Pereira, Patrícia; Barreira, Maria; Cruz, Carla; Tomás, Joana; Luís, Ângelo; Pedro, Augusto Q.; Queiroz, João A.; Sousa, Fani. "Brain-Targeted Delivery of Pre-miR-29b Using Lactoferrin-Stearic Acid-Modified-Chitosan/Polyethyleneimine Polyplexes". Pharmaceuticals 13 10 (2020): 314. http://dx.doi.org/10.3390/ph13100314.
    10.3390/ph13100314
  9. Castro, Leonor S.; Pereira, Patrícia; Passarinha, Luís A.; Freire, Mara G.; Pedro, Augusto Q.. "Enhanced performance of polymer-polymer aqueous two-phase systems using ionic liquids as adjuvants towards the purification of recombinant proteins". Separation and Purification Technology 248 (2020): 117051. http://dx.doi.org/10.1016/j.seppur.2020.117051.
    Published • 10.1016/j.seppur.2020.117051
  10. Cruz, Carla; Pedro, Augusto Q.; Carvalho, Josué; Santos, Tiago; Talhada, Daniela; Paiva, Artur; Queiroz, João A.; et al. "Nucleolin as a potential biomarker for canine malignant neoplasia". Research in Veterinary Science (2020): http://dx.doi.org/10.1016/j.rvsc.2020.10.004.
    10.1016/j.rvsc.2020.10.004
  11. Neves, M.C.; Pereira, P.; Pedro, A.Q.; Martins, J.C.; Trindade, T.; Queiroz, J.A.; Freire, M.G.; Sousa, F.. "Improved ionic-liquid-functionalized macroporous supports able to purify nucleic acids in one step". Materials Today Bio 8 (2020): 100086. http://dx.doi.org/10.1016/j.mtbio.2020.100086.
    Published • 10.1016/j.mtbio.2020.100086
  12. Azevedo, G.M.; Valente, J.F.A.; Sousa, A.; Pedro, A.Q.; Pereira, P.; Sousa, F.; Queiroz, J.A.. "Effect of Chromatographic Conditions on Supercoiled Plasmid DNA Stability and Bioactivity". Applied Sciences 9 23 (2019): 5170. http://dx.doi.org/10.3390/app9235170.
    Open access • Published • 10.3390/app9235170
  13. Pedro, Augusto Quaresma; Queiroz, João António; Passarinha, Luís António. "Smoothing membrane protein structure determination by initial upstream stage improvements". Applied Microbiology and Biotechnology 103 14 (2019): 5483-5500. http://dx.doi.org/10.1007/s00253-019-09873-1.
    Published • 10.1007/s00253-019-09873-1
  14. Augusto Pedro. "Spectroscopic studies of naproxen and tryptophan immobilized in polyvinyl alcohol". Biotechnology and Food Science (2019): Tom 75 Nr 1 (2011):-Tom 75 Nr 1 (2011):. https://eczasopisma.p.lodz.pl/BFS/article/view/9.
    10.34658/BFS.2011.75.1.39-49
  15. Quental, M.V.; Pedro, A.Q.; Pereira, P.; Sharma, M.; Queiroz, J.A.; Coutinho, J.A.P.; Sousa, F.; Freire, M.G.. "Integrated Extraction-Preservation Strategies for RNA Using Biobased Ionic Liquids". ACS Sustainable Chemistry and Engineering 7 10 (2019): 9439-9448. https://pubs.acs.org/doi/10.1021/acssuschemeng.9b00688.
    Published • 10.1021/acssuschemeng.9b00688
  16. Pedro, A.Q.; Gonçalves, A.M.; Queiroz, J.A.; Passarinha, L.A.. "Purification of Histidine-Tagged Membrane-Bound Catechol-O-Methyltransferase from Detergent-Solubilized Pichia pastoris Membranes". Chromatographia 81 3 (2018): 425-434. https://link.springer.com/article/10.1007/s10337-017-3453-5.
    Published • 10.1007/s10337-017-3453-5
  17. Pedro, A.Q.; Pereira, P.; Quental, M.J.; Carvalho, A.P.; Santos, S.M.; Queiroz, J.A.; Sousa, F.; Freire, M.G.. "Cholinium-Based Good's Buffers Ionic Liquids as Remarkable Stabilizers and Recyclable Preservation Media for Recombinant Small RNAs". ACS Sustainable Chemistry and Engineering 6 12 (2018): 16645-16656. https://pubs.acs.org/doi/10.1021/acssuschemeng.8b03900.
    Published • 10.1021/acssuschemeng.8b03900
  18. Pereira, P.; Pedro, A.Q.; Queiroz, J.A.; Figueiras, A.R.; Sousa, F.. "New insights for therapeutic recombinant human miRNAs heterologous production: Rhodovolum sulfidophilum vs Escherichia coli". Bioengineered 8 5 (2017): 670-677. https://www.tandfonline.com/doi/full/10.1080/21655979.2017.1284710.
    Published • 10.1080/21655979.2017.1284710
  19. Pereira P; Pedro AQ; Tomás J; Maia CJ; Queiroz JA; Figueiras A; Sousa F. "Advances in time course extracellular production of human pre-miR-29b from Rhodovulum sulfidophilum". (2016): https://link.springer.com/article/10.1007/s00253-016-7350-x.
    Published • 10.1007/s00253-016-7350-x
  20. Augusto Q. Pedro; Filipa F. Correia; Fátima M. Santos; Guilherme Espírito-Santo; Ana M. Gonçalves; Maria J. Bonifacio; João António de Sampaio Rodrigues Queiroz; Luís A. Passarinha. "Biosynthesis and purification of histidine-tagged human soluble catechol-O-methyltransferase". Journal of Chemical Technology and Biotechnology 91 12 (2016): 3035-3044. https://onlinelibrary.wiley.com/doi/full/10.1002/jctb.4930.
    Published • 10.1002/jctb.4930
  21. Pedro, A.Q.; Pereira, P.; Bonifácio, M.J.; Queiroz, J.A.; Passarinha, L.A.. "Purification of Membrane-Bound Catechol-O-Methyltransferase by Arginine-Affinity Chromatography". Chromatographia 78 21-22 (2015): 1339-1348. https://link.springer.com/article/10.1007/s10337-015-2970-3.
    Published • 10.1007/s10337-015-2970-3
  22. Pedro, A.Q.; Martins, L.M.; Dias, J.M.L.; Bonifácio, M.J.; Queiroz, J.A.; Passarinha, L.A.. "An artificial neural network for membrane-bound catechol-O-methyltransferase biosynthesis with Pichia pastoris methanol-induced cultures". Microbial Cell Factories 14 113 (2015): https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-015-0304-7.
    Open access • Published • 10.1186/s12934-015-0304-7
  23. Martins, L.M.; Pedro, A.Q.; Oppolzer, D.; Sousa, F.; Queiroz, J.A.; Passarinha, L.A.. "Enhanced biosynthesis of plasmid DNA from Escherichia coli VH33 using Box-Behnken design associated to aromatic amino acids pathway". Biochemical Engineering Journal 98 (2015): 117-126. https://www.sciencedirect.com/science/article/pii/S1369703X15000248.
    Published • 10.1016/j.bej.2015.02.001
  24. Pedro, A.Q.; Oppolzer, D.; Bonifácio, M.J.; Maia, C.J.; Queiroz, J.A.; Passarinha, L.A.. "Evaluation of MutS and Mut+ Pichia pastoris Strains for Membrane-Bound Catechol-O-Methyltransferase Biosynthesis". Applied Biochemistry and Biotechnology 175 8 (2015): 3840-3855. https://link.springer.com/article/10.1007%2Fs12010-015-1551-0.
    Published • 10.1007/s12010-015-1551-0
  25. Correia, F.F.; Santos, F.M.; Pedro, A.Q.; Bonifácio, M.J.; Queiroz, J.A.; Passarinha, L.A.P.. "Recovery of biological active catechol-O-methyltransferase isoforms from Q-sepharose". Journal of Separation Science 37 1-2 (2014): 20-29. https://onlinelibrary.wiley.com/doi/abs/10.1002/jssc.201300977.
    Published • 10.1002/jssc.201300977
  26. Pedro, A.Q.; Soares, R.F.;; Oppolzer, D.;; Santos, F.M.;; Rocha, L.A.;; Gonçalves, A.M.;; Bonifácio, M.J.;; et al. "An Improved HPLC Method for Quantification of Metanephrine with Coulometric Detection". Journal of Chromatography & Separation Techniques 05 02 (2014): http://dx.doi.org/10.4172/2157-7064.1000217.
    Published • 10.4172/2157-7064.1000217
  27. Gonçalves, A.M.; Pedro, A.Q.; Santos, F.M.; Martins, L.M.; Maia, C.J.; Queiroz, J.A.; Passarinha, L.A.. "Trends in protein-based biosensor assemblies for drug screening and pharmaceutical kinetic studies". Molecules 19 8 (2014): 12461-12485. https://doi.org/10.3390/molecules190812461.
    Open access • Published • 10.3390/molecules190812461
  28. Espírito Santo, G.M.; Pedro, A.Q.; Oppolzer, D.; Bonifácio, M.J.; Queiroz, J.A.; Silva, F.; Passarinha, L.A.. "Development of fed-batch profiles for efficient biosynthesis of catechol-O-methyltransferase". Biotechnology Reports 3 (2014): 34-41. https://www.sciencedirect.com/science/article/pii/S2215017X14000083.
    Published • 10.1016/j.btre.2014.05.005
  29. Gonçalves, A.M.; Pedro, A.Q.; Maia, C.; Sousa, F.; Queiroz, J.A.; Passarinha, L.A.. "Pichia pastoris: A recombinant microfactory for antibodies and human membrane proteins". Journal of Microbiology and Biotechnology 23 5 (2013): 587-601. http://www.jmb.or.kr/journal/viewJournal.html?doi=JMB023-05-01.
    Published • 10.4014/jmb.1210.10063
  30. Santos, F.M.; Pedro, A.Q.; Soares, R.F.; Martins, R.; Bonifácio, M.J.; Queiroz, J.A.; Passarinha, L.A.. "Performance of hydrophobic interaction ligands for human membrane-bound catechol-O-methyltransferase purification". Journal of Separation Science 36 11 (2013): 1693-1702. https://onlinelibrary.wiley.com/doi/10.1002/jssc.201300010.
    Published • 10.1002/jssc.201300010
  31. Pedro, A.Q.; Bonifácio, M.J.; Queiroz, J.A.; Maia, C.J.; Passarinha, L.A.. "A novel prokaryotic expression system for biosynthesis of recombinant human membrane-bound catechol-O-methyltransferase". Journal of Biotechnology 156 2 (2011): 141-146. https://www.sciencedirect.com/science/article/pii/S0168165611004925.
    Published • 10.1016/j.jbiotec.2011.08.022
Magazine article
  1. Leonor S. Castro, Patrícia Pereira, Mara G. Freire, Augusto Q. Pedro. "Progress in the development of aqueous two-phase systems comprising ionic liquids for the downstream processing of protein-based biopharmaceuticals (Invited)", American Pharmaceutical review, 2019, https://www.americanpharmaceuticalreview.com/Featured-Articles/518305-Progress-in-the-Development-of-Aqueous-Two-Phase-Systems-Comprising-Ionic-Liquids-for-the-Downstream-Processing-of-Protein-Based-Biopharmaceuticals/.
Newsletter article
  1. Sabara D M, Dunne JS, Pedro A Q, Trifunovic J, Balogun I I O, Kotlicic O, Serrano A, Kertez, I. "Spectroscopic studies of naproxen and tryptophan immobilized in polyvinyl alcohol", Biotechnology and Food Science, 2011, http://yadda.icm.edu.pl/yadda/element/bwmeta1.element.baztech-article-LOD7-0032-0046.

Intellectual property

Patent
  1. Mara Guadalupe Freire Martins; Augusto Quaresma Henriques Pedro; Coutinho, Joao A. P.; Ana Maria Ferreira; Mukesh Sharma; Dibyendu Mondal; Maria João Quental. 2021. "Sistema aquoso bifásico para uso em medicina veterinária, métodos de produção e suas aplicações". Portugal.
    Granted/Issued
  2. Augusto Q. Pedro; Mara G. Freire; Patricia Pereira; Márcia C. Neves; João C. Martins; Fani Sousa; João António de Sampaio Rodrigues Queiroz. 2019. "Separação de ácidos nucleicos utilizando líquidos iónicos como ligandos cromatográficos multimodais em matriz macroporosas (PT 110626 A)". Portugal.
    Granted/Issued
Provisional application for patent
  1. 2019. "Separation of nucleic acids using chromatographic matrices functionalized with ionic liquids (Filled WO/2019/175849 A1)".
    Protected
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2019/11/22 Improved downstream processing of recombinant proteins using aqueous two-phase systems composed of ionic liquids Protein Engineering & Summit Europe (PEGS Europe)
Cambridge Healthtech Institute (Lisboa, Portugal)
2019/09/10 Downstream processing of recombinant proteins using alternative purification platforms based on ionic liquids ILSEPT – 4th International Conference on Ionic Liquids in Separation and Purification Tehcnology
(Sitges, Spain)
2019/07/04 Ionic liquids-based supports for the purification of RNA XIV Annual CICS-UBI Symposium
Centro de Investigação em Ciências da Saúde - Universidade da Beira Interior (Covilhã, Portugal)
2019/06/12 Bioprocessing of recombinant proteins using alternative purification platforms Spanish National Congress of Biotechnology (BIOTEC)
Spanish Society of Biotechnology (Vigo, Spain)
2018/06/09 Circulating nucleolin as a potential biomarker for canine tumors XXIII Meeting of the Portuguese Society Animal Pathology
Universidade de Coimbra (Coimbra, Portugal)
2017/09/24 Functionalization of chromatographic matrices with ionic liquids for RNA purification Biopartitioning and Purification (BPP)
Technical University of Copenhagen (Copenhaga, Denmark)
2017/07/06 Synthesis and characterization of ionic liquid functionalized matrices for RNAs purification XII Annual CICS-UBI Symposium
Centro de Investigação em Ciências da Saúde - Universidade da Beira Interior (Covilhã, Portugal)
2017/05/17 Small RNA stabilization using biocompatible cholinium-based ionic liquids II International Congress in Health Sciences Research: Towards Innovation and Entrepreneurship
Centro de Investigação em Ciências da Saúde - Universidade da Beira Interior (Covilhã, Portugal)
2017/05/17 Novel alternatives for interferon alpha2b purification: chromatography vs ionic liquid aqueous biphasic systems II International Congress in Health Sciences Research: Towards Innovation and Entrepreneurship
Centro de Investigação em Ciências da Saúde - Universidade da Beira Interior (Covilhã, Portugal)
2017/04/06 Modern applications of ionic liquids in biomolecules bioprocessing: a case-study involving small RNA stabilization using cholinium-based ionic liquids (Invited) III Cycle of Conferences in Medicinal and Pharmaceutical Biotechnology
Universidade da Beira Interior (Covilhã, Portugal)
2015/11/10 A biotechnological platform for pre-miRNAs biosynthesis, purification and brain-targeted delivery Microbiotec'15 - Congress of Microbiology and Biotechnology
Universidade de Évora (Évora, Portugal)
2015/07 Biosynthesis, purification and brain-targeted delivery of pre-miR-29b biopharmaceuticals for Alzheimer’s disease X Annual CICS Symposium
Centro de Investigação em Ciências da Saúde (C, Portugal)
2013/07/01 Purification of recombinant human membrane COMT by ionic-exchange chromatography VIII Annual CICS-UBI Symposium
Centro de Investigação em Ciências da Saúde (Covilhã, Portugal)
2012/09/25 A novel prokaryotic expression system for biosynthesis of recombinant human membrane-bound catechol-O-methyltransferase 9th European Symposium on Biochemical Engineering Science (ESBES).
ESBES (Istambul, Turkey)
2012/09/24 Evaluation of human membrane-bound catechol-O-methyltransferase purification by hydrophobic interaction 32nd International Symposium on the Separation of Peptides, Proteins, and Polynucleotides (ISPPP)
(Istambul, Turkey)
2012/02/01 Kinetics of a recombinant form of human membrane-bound Catechol-O-methyltransferase XLII Portuguese Society Pharmacology Reunion
Portuguese Society Pharmacology (Lisboa, Portugal)
2011/07/05 Advances in time-course biosynthesis and solubilisation of MBCOMT from Brevibacillus cells VI Annual CICS-UBI Symposium
Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior (Covilhã, Portugal)

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2023/08 - Current Production and clarification of mRNA vaccines using integrated processes based on thermoreversible aqueous biphasic systems and ionic liquids (FCT, 2022.11476.BD)
Supervisor
Universidade de Aveiro, Portugal

Universidade de Aveiro CICECO, Portugal
2022/10 - Current Melhorias na obtenção de vacinas de ARN mensageiro utilizando processos integrados de produção-clarificação
Supervisor
 Biotecnologia (Master)
Universidade de Aveiro, Portugal
2022/10 - Current RECOMBINANT MEMBRANE PROTEIN PRODUCTION USING INTEGRATED STRATEGIES
Supervisor
 Biotecnologia (Master)
Universidade de Aveiro, Portugal
2021 - Current Downstream processing of therapeutic proteins using hybrid technologies based on aqueous two-phase systems and supported ionic liquids
Co-supervisor
 Engenharia Química (PhD)
Universidade de Aveiro, Portugal

Universidade de Aveiro CICECO, Portugal
2022/03/15 - 2022/06/30 PhD Contract within the scope of I&D IL2BioPro Melhorias no bioprocessamento de proteínas recombinantes utilizando líquidos iónicos (POCI-01-0145-FEDER-030840)
Supervisor
Universidade de Aveiro, Portugal
2022/03/15 - 2022/06/15 Desenvolvimento de estratégias integradas para a recuperação da ciclooxigenase-2 utilizando líquidos iónicos - Master Fellowship (POCI-01-0145-FEDER-030840)
Supervisor
Universidade de Aveiro, Portugal
2022/02 - 2022/06 Novas aplicações de fibrilas proteicas em bioengenharia
Supervisor of Francisco Rocha
 Biotecnologia (Degree)
Universidade de Aveiro, Portugal
2022/02 - 2022/06 Desenvolvimento de estratégias integradas para a clarificação e purificação de proteínas terapêuticas recombinantes
Supervisor
 Biotecnologia (Degree)
Universidade de Aveiro, Portugal
2021/04 - 2021/07 Purification of protein-based biopharmaceuticals using thermo-responsive and biocompatible polymers
Supervisor of Hugo Miguel Dores da Silva
 Biotecnologia Medicinal (Degree)
Instituto Politécnico do Porto, Portugal
2021/04 - 2021/07 Integrated recovery-purification strategies of protein-based biopharmaceuticals
Supervisor
 Biotecnologia Medicinal (Degree)
Instituto Politécnico do Porto, Portugal
2021/02 - 2021/07 Inhibition and disaggregation of beta-amyloid fibrils from Alzheimer's disease using ionic liquids
Supervisor
 Biotecnologia (Degree)
Universidade de Aveiro, Portugal
2021/02 - 2021/07 Inibição e desagregação de fibrilas beta-amilóides da doença de Alzheimer utilizando solventes eutécticos profundos
Supervisor
 Biotecnologia (Degree)
Universidade de Aveiro, Portugal
2021/02 - 2021/06 Libertação controlada de biofármacos de DNA plasmídico por fibrilas de albumina humana
Supervisor
 Bioquímica (Degree)
Universidade de Aveiro, Portugal
2019 - 2021 Chromatographic bioseparations of therapeutic proteins using ionic-liquid-functionalized silicas
Co-supervisor of Guilherme Lobo e Sousa
Bioquímica (Master)
Universidade de Aveiro, Portugal
2019 - 2020 IL2BioPro: Towards the improvement of recombinant proteins bioprocessing using ionic liquids - Master Fellowship
Supervisor of Leonor Sofia de Oliveira e Castro
2016 - 2017/10/13 Produção e Purificação do Interferão Alfa-2b a partir de Culturas de Escherichia coli Plataformas Alternativas
Co-supervisor of Joana Raquel Amaral Antunes
 Biotecnologia (Master)
Universidade de Aveiro, Portugal
2014 - 2015/11/15 Lyophilization and a preliminary thermodynamic characterization of recombinant SCOMT_His6
Co-supervisor of Rúben Miguel Oliveira Coval
 Biotecnologia (Master)
Universidade da Beira Interior, Portugal
2014 - 2015 Reconstituição funcional da isoforma membranar da catecol-O-metiltransferase humana em sistemas não micelares
Supervisor of Maria João Abrantes
 Bioengenharia (Degree)
Universidade da Beira Interior, Portugal
2013 - 2014/07/16 Stabilization of COMT by experimental design approaches
Co-supervisor of Diana Margarida Tavares de Oliveira
 Biotecnologia (Master)
Universidade da Beira Interior, Portugal

Event organisation

Event name
Type of event (Role) 		Institution / Organization
2013/12/02 - 2013/12/04 8º Encontro Nacional de Cromatografia (2013/12/02 - 2013/12/04)
Congress (Member of the Organising Committee)
 Universidade da Beira Interior, Portugal
2013/07/01 - 2013/07/02 VIII Annual CICS-UBI Symposium (2013/07/01 - 2013/07/02)
Symposium (Member of the Organising Committee)
 Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal

Event participation

Activity description
Type of event 		Event name
Institution / Organization
2019/07/15 - 2019/07/19 Theoretical and Practical course on cell culture
Workshop
 VII Cell Culture and Tissue Engineering Training Course “Beyond the Microscope, culturing life”
Universidade de Coimbra Faculdade de Medicina, Portugal

Instituto Politécnico de Coimbra Escola Superior de Tecnologia da Saúde de Coimbra, Portugal
2018/01/11 - 2018/01/11 Theoretical course on the application of Good Manufacturing Practices (GMP) rules for (bio)pharmaceutical applications
Symposium
 Good Manufacturing Practices in the Pharmaceutical Industry: how transform rules into productivity tools
Universidade da Beira Interior, Portugal
2013/10/21 - 2013/10/25 Theoretical and Practical course on the synthesis and purification of recombinant proteins, and modern biophysical techniques to study protein-ligand interactions
Workshop
 EMBO Practical course on modern biophysical methods for protein-ligand interactions
Oulun Yliopisto, Finland

EMBO Press, Germany
2012/02/19 - 2012/02/23 Theoretical and Practical Course on Proteomics
Workshop
 Three Day Proteomics Course: From 2DE to protein identification by mass spectrometry
Centro de Ciências do Mar, Portugal
2012/02/13 - 2012/02/17 Theoretical and Practical course on the application of NMR for molecules identification and studying small-molecule-protein interaction
Workshop
 Hands on course, NMR – From theory to practise
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal

Jury of academic degree

Topic
Role 		Candidate name (Type of degree)
Institution / Organization
2018/11/07 Molecular Modelling of a novel G-quadruplex structure and its interaction with ligands
(Thesis) Main arguer
 Rui Pedro Abrantes Carrilho (Master)
Universidade da Beira Interior, Portugal
2018/07/20 Novas estratégias de purificação de microRNAs usando líquidos iónicos
(Thesis) Main arguer
 Inês Isabel Dias Rodrigues (Master)
Universidade da Beira Interior, Portugal
2017/11/10 Novas estratégias de purificação de RNAs usando líquidos iónicos
(Thesis) Main arguer
 João Carlos Claudino Martins (Master)
Universidade da Beira Interior, Portugal
2016/11/14 Biosynthesis optimization of STEAP1 protein in Pichia pastoris X33 MUT+
(Thesis) Main arguer
 Margarida Maria Antunes dos Santos (Master)
Universidade da Beira Interior, Portugal
2016/07/13 Isolation and purification of STEAP1 protein fragment in Escherichia Coli cells: a potential target for prostate cancer
(Thesis) Main arguer
 Jorge Daniel Barroca Ferreira (Master)
Universidade da Beira Interior, Portugal
2014 Caracterização biofísica e bioquímica da MBCOMT ligada a um inibidor farmacológico
(Thesis) Main arguer
 Rita Monteiro (Other)
Universidade da Beira Interior, Portugal
2014 Protótipo de uma mitocôndria artificial para aplicação na doença de Alzheimer
(Thesis) Main arguer
 Joana Coelho (Other)
Universidade da Beira Interior, Portugal
2014 Biosíntese, purificação e biointeracção da proteína humana recombinante Tau com inibidores da doença de Alzheimer
(Thesis) Main arguer
 Luís Clara (Other)
Universidade da Beira Interior, Portugal

Association member

Society Organization name Role
2023/01 - Current UAVR-RNA Club Co-founder

Committee member

Activity description
Role 		Institution / Organization
2016/09 - Current Member of Scientific Council of Aveiro Institute of Materials (CICECO)/University of Aveiro
Member
 Universidade de Aveiro CICECO, Portugal

Course / Discipline taught

Academic session Degree Subject (Type) Institution / Organization
2021/09 - 2022/02 Laboratory classes of microbial biotechnology Biotecnologia (Licenciatura) Universidade de Aveiro Departamento de Química, Portugal
2021/02 - 2021/06 Laboratory classes of general chemistry. Meteorologia, Oceanografia e Geofísica (Licenciatura) Universidade de Aveiro, Portugal
2017 - 2018 Seminars in Biotechnology (Invited Speaker) Biotecnologia (Mestrado) Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2013 - 2016 Biotechnology - Practical (Laboratory) SDS-PAGE Classes Bioquímica (Mestrado) Universidade da Beira Interior, Portugal
2013 - 2016 Biotechnology - Practical (Laboratory) SDS-PAGE Classes Ciências Biomédicas (Mestrado) Universidade da Beira Interior, Portugal
2013 - 2016 Biotechnology - Practical (Laboratory) SDS-PAGE Classes Química Medicinal (Mestrado) Universidade da Beira Interior, Portugal
2012 - 2016 Cellular and Molecular Biology - Practical (Laboratory) Classes Bioengenharia (Licenciatura) Universidade da Beira Interior, Portugal
2012 - 2016 Cellular and Molecular Biology - Practical (Laboratory) Classes Biotecnologia (Licenciatura) Universidade da Beira Interior, Portugal
2012 - 2016 Cellular and Molecular Biology - Practical (Laboratory) Classes Optometria - Ciências da Visão (Licenciatura) Universidade da Beira Interior, Portugal

Mentoring / Tutoring

Topic Student name
2017 - 2018 Purification of nucleic acids using macroporous chromatographic matrices modified with ionic liquids; João C. Martins
2012 - 2013 Bacterial production of plasmid DNA biopharmaceuticals; Luís M. C. Martins
2012 - 2013 Chromatographic purification of recombinant proteins and determination of enzymatic activities; Filipa I. F. Correia
2012 - 2013 Recombinant production of proteins; Guilherme M. P. R. Espírito-Santo
2011 - 2012 Chromatographic purification of recombinant proteins and determination of enzymatic activities; Fátima M. Santos
Distinctions

Award

2019 Best short communication "Ionic liquids-based supports for the purification of RNA"
Universidade da Beira Interior, Portugal
2015 Best Oral Communication "Biosynthesis, purification and brain-targeted delivery of pre-miR-29b biopharmaceuticals for Alzheimer’s disease therapy"
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2012 Best Poster Communication "Strategies to improve recombinant human membrane protein overexpression for structural, functional and clinical studies"
Instituto Politécnico da Guarda, Portugal