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Identification

Personal identification

Full name
Ana Sofia Afonso Ferreira

Author identifiers

Ciência ID
721A-AA1B-6660

Email addresses

  • anasofia.afonsoferreira@gmail.com (Professional)
Education
Degree Classification
2011
Concluded
 Ciências Veterinárias (Doutoramento)
Major in Sem especialidade
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
"Molecular and serological approaches for the detection and typing of lepstospira " (THESIS/DISSERTATION)
2009
Concluded
 Biologia Clínica Laboratorial (Mestrado)
Universidade de Trás-os-Montes e Alto Douro, Portugal
"Infecções Fúngicas - Diagnóstico e Epidemiologia" (THESIS/DISSERTATION)
 18 valores
2007
Concluded
 Biologia (Licenciatura)
Universidade de Trás-os-Montes e Alto Douro, Portugal
"Caracterização de Isolados Ambientais de Cryptococcus neoformans e sua Comparação com Isolados Clínicos" (THESIS/DISSERTATION)
 15 valores
Affiliation

Teaching in Higher Education

Category
Host institution 		Employer
2023/09/01 - Current Invited Associate Professor (University Teacher) Cooperativa de Ensino Superior Politécnico e Universitário CRL, Portugal

Others

Category
Host institution 		Employer
2021/02/01 - Current Integrated Member (PhD) Universidade do Porto Instituto de Saúde Pública, Portugal
Universidade do Porto Faculdade de Farmácia, Portugal
2017/07/01 - Current Collaborator in the Microbiology Laboratory of the Biological Sciences (Virology), Department of the Faculty of Pharmacy Universidade do Porto, Portugal
2009/12/01 - 2015/12/14 PhD student Instituto Nacional de Investigação Agrária e Veterinária, Portugal
2012/10/01 - 2012/12/22 Internship Koninklijk Instituut voor de Tropen, Netherlands
2007/12/03 - 2009/05/20 MSc student Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2007/02/01 - 2009/05/20 Technical and Scientific Community Service Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2007/02/01 - 2007/09/30 BSc student Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
Projects

Grant

Designation Funders
2009/12/01 - 2014/09/01 Molecular and Serological approaches for the Detection and Typing of Leptospira
SFRH/BD/62317/2009
PhD Student Fellow
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal

Instituto Nacional de Investigação Agrária e Veterinária, Portugal
Fundação para a Ciência e a Tecnologia
Concluded

Other

Designation Funders
2020/06/01 - 2020/08/31 SARS-CoV-2 and blood donation safety in Portugal: a shift to screening? SARSBLOODsafe
021_596187412
Researcher
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
Outputs

Publications

Book chapter
  1. Cruz, R.; Vasconcelos-Nobrega, Carmen; Esteves, F.; Coelho, C.; Ferreira, AS; Santos, C.; Mega, C.; et al. "A One Health Perspective on Q Fever: Part 2". 195-216. Hershey, PA: IGI Global, 2019.
    10.4018/978-1-5225-6304-4.ch009
  2. Cruz, R.; Vasconcelos-Nobrega, C.; Esteves, F.; Coelho, C.; Ferreira, AS; Santos, C.; Mega, C.; et al. "A One Health Perspective on Q Fever: Part 1". In Global Applications of One Health Practice and Care, 174-194. Hershey, PA: IGI Global, 2018.
    Published • 10.4018/978-1-5225-6304-4.ch008
  3. Ferreira, Ana; Paiva-Cardoso, MDN; Thompson, G; Inácio, J. "Reação de polimerase em cadeia em tempo real (real time PCR) com sondas múltiplas: Deteção de Leptospira em amostras biológicas de animais". In Abordagens Moleculares em Veterinária - Como desvendar a etiologia, a epidemiologia e os mecanismos da infeção. Lidel - Edições Técnicas Lda., 2014.
    Published
  4. Ferreira, AS; Ahmed, A.; Hartskeerl, R.A.. "Multilocus sequence typing (MLST): markers for the traceability of pathogenic Leptospira strains". In Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies. Methods in Molecular Biology (Methods and Protocols), edited by Monica Cunha; João José Inácio Silva. New York, NY, United States: Humana Press, 2014.
    10.1007/978-1-4939-2004-4_25.
Conference poster
  1. Santos-Ferreira, NL; Ferreira, AS; Mesquita, JR; Nascimento, MSJ. "Evidência serológica de infeção humana pelo vírus do Nilo Ocidental em Portugal". Paper presented in V Congresso Nacional de Virologia – IX Encontro da Sociedade Portuguesa de Virologia, 2018.
  2. Mesquita, JR; Istrate, C; Santos-Ferreira, NL; Ferreira, AS; Veiga, J; van der Poel, WHM; Nascimento, MSJ. "Deteção e caracterização molecular do vírus da hepatite E em animais domésticos de São Tomé e Príncipe". Paper presented in V Congresso Nacional de Virologia – IX Encontro da Sociedade Portuguesa de Virologia, 2018.
  3. Cruz, R; Esteves, F; Vasconcelos-Nobrega, C; Santos, C; Ferreira, AS; Mega, C; Coelho, AC; Vala, H; Mesquita, JR. "Serological evidence of West Nile virus infection in the Portuguese population". Paper presented in 21st ESCV-European Society for Clinical Virology, 2018.
  4. Inácio, J; Amaro, A; Ferreira, AS; Albuquerque, T; Cunha, MV; Couto, I; Botelho, A; Viveiros, M; Costa, P. "Enhanced detection and identification of tuberculous mycobacteria using 16S rDNA, IS6110 and Regions of Difference-targeted dual-labelled hydrolysis probes". Paper presented in VI Congresso da Sociedade Portuguesa de Ciências Veterinárias - CCV2014, 2014.
  5. Costa, P; Ferreira, AS; Amaro, A; Albuquerque, T; Botelho, A; Couto, I; Cunha, MV; Viveiros, M; Inácio, J. "Direct detection in animal tissues of mycobacteria belonging to the Mycobacterium tuberculosis complex using a semi-nested probe-based real-time PCR". Paper presented in Clinical Microbiology. MICROBIOTEC’13, 2013.
  6. Costa, P; Ferreira, A; Amaro, A; Couto, I; Viveiros, M; Inácio, J. "Evaluation of the presence of mycobacteria belonging to the Mycobacterium tuberculosis complex in animal tissues by real-time PCR". Paper presented in MDR Diagnostic and DST. 34th Congress of European Society of Mycobacteriology (ESM), 2013.
  7. Pereira, C; Costa, P; Ferreira, A; Mendonça, P; Carvalho, P; Monteiro, M; Ferronha, H.; Inácio, J; Amaro, A. "Deteção por hibridação in situ e PCR em tempo real de micobactérias do Complexo Mycobacterium tuberculosis em secções histológicas". Paper presented in 2.º Encontro de Biologia Molecular em Saúde, 2012.
  8. Costa, P; Pereira, C; Soares, R; Ferreira, A; Ferronha, H; Gomes, J; Inácio, J; Amaro, A. "Evaluation of IS6110-targeted conventional and real time PCR assays for detecting Mycobacterium tuberculosis Complex in paraffin embedded bovine tissues". Paper presented in Molecular Microbiology and Microbial Physiology. MICROBIOTEC’11, 2011.
  9. Ferreira, Ana; Costa, P; Amaro, A; Rocha, T; Thompson, G; Inácio, J.. "Novel TaqMan-based multiplex real-time PCR assays to detect and discriminate pathogenic Leptospira genomospecies". Paper presented in Molecular Microbiology and Microbial Physiology. MICROBIOTEC’11, 2011.
  10. Costa, P; Ferreira, A; Amaro, A; Botelho, A; Inácio, J. "Rapid detection and identification of Mycobacterium bovis and other M. tuberculosis complex members using a TaqMan-based real-time PCR approach". Paper presented in V Congresso da Sociedade Portuguesa de Ciências Veterinárias - CCV2011, 2011.
  11. Ferreira, Ana; Costa, P; Amaro, A; Rocha, T; Thompson, G; Inácio, J. "Detection and discrimination of pathogenic Leptospira genomospecies in single-tube multiplex real-time PCR assays". Paper presented in V Congresso da Sociedade Portuguesa de Ciências Veterinárias - CCV2011, 2011.
  12. Costa, P; Ferreira, A; Amaro, A; Botelho, A; Viveiros, M; Inácio, J. "Detection and identification of Mycobacterium bovis and other M. tuberculosis complex members using Regions of Difference targeted TaqMan probes". Paper presented in : Molecular Methods for Diagnosis of Infective Agents. 4th Congress of European Microbiologists - FEMS 2011, 2011.
  13. Ferreira, Ana; Costa, P; Thompson, G; Inácio, J.. "Improved multigene targets for the Taqman-based identification of pathogenic Leptospira species". Paper presented in Molecular Methods for Diagnosis of Infective Agents. 4th Congress of European Microbiologists - FEMS 2011, 2011.
  14. Ferreira, Ana; Maduro, AP; Martins, ML; Inácio, J; Sampaio, A. "Zoonotic potential of cryptococcosis associated with domestic pigeons: molecular heterogeneity amongst Cryptococcus neoformans isolates of human and animal origins". Paper presented in I Jornadas de Saúde Pública Veterinária da UTAD, 2010.
  15. Ferreira, AS; Teodósio, R; Martins, ML. "Tinea capitis caused by dermatophytes of african origin: a health problem in a suburb slum of Lisbon". Paper presented in Migrants and Refugees. 11th Conference of the International Society of Travel Medicine, 2009.
  16. Bastos, R; Ferreira, AS; Franco, N. "Desenvolvimento de um modelo ecológico para a previsão de tendências populacionais do Priolo (Pyrrhula murina) em São Miguel – Açores". Paper presented in XI Encontro Nacional de Estudantes de Biologia “Interacções Biológicas”, 2007.
Journal article
  1. Mesquita, João R.; Barradas, Patrícia; Gomes da Silva, Priscilla; Ferreira, Ana Sofia; Silva, Eliane; Matas, Isabel M.; Thomson, Gertrude; et al. "SARS-CoV-2 and blood donations in Portugal, June–July 2020". (2021):
    10.1002/jmv.27353
  2. João Inácio; Antonio Muñoz-Mérida; Ahmed A. Ahmed; Teresa Rocha; João Rodrigo Mesquita; Gertrude Thompson; Marga G. A. Goris; et al. "Draft Genome Sequence of a Leptospira kirschneri Serovar Mozdok Type 2 Strain Isolated from a Horse in Portugal". Microbiology Resource Announcements 10 28 (2021): https://doi.org/10.1128/MRA.00217-21.
    10.1128/MRA.00217-21
  3. Cruz, Rita; Santos, Carla; Esteves, Fernando; Ferreira, Ana Sofia; Vasconcelos-Nóbrega, Carmen; Mega, Cristina; Albuquerque, Carlos; et al. "Occupationally Acquired Q Fever in Shepherds and Sheep Milk Cheesemakers". EcoHealth (2020): http://dx.doi.org/10.1007/s10393-020-01483-y.
    10.1007/s10393-020-01483-y
  4. Mesquita, João R.; Santos-Ferreira, Nanci; Ferreira, Ana S.; Albuquerque, Carlos; Nóbrega, Cármen; Esteves, Fernando; Cruz, Rita; Vala, Helena; Nascimento, Maria S. J.. "Increased risk of hepatitis E virus infection in workers occupationally exposed to sheep". Transboundary and Emerging Diseases (2020): http://dx.doi.org/10.1111/tbed.13524.
    10.1111/tbed.13524
  5. Ferreira, AS. "Genetic diversity of pathogenic leptospires from wild, domestic and captive host species in Portugal". Transboundary and Emerging Diseases (2019): http://dx.doi.org/10.1111/tbed.13409.
    10.1111/tbed.13409
  6. Esteves, Fernando; Cruz, Rita; Vasconcelos-Nóbrega, Cármen; Santos, Carla; Ferreira, Ana S.; Petrovic, Tamas; Cardoso, Luís; et al. "Bulk-Tank Milk Longitudinal Serosurvey Reveals Decreasing Schmallenberg Virus Circulation in a Confined Population of Portuguese Sheep". Vector-Borne and Zoonotic Diseases (2019): http://dx.doi.org/10.1089/vbz.2018.2420.
    10.1089/vbz.2018.2420
  7. Cruz, R; Esteves, F; Vasconcelos-Nobrega, C; Santos, C; Ferreira, AS; Mega, C; Coelho, AC; Vala, H; Mesquita, JR. "Prospective Serosurvey of Coxiella burnetii Antibodies in Selected Sheep of Portugal". (2018):
    10.1007/s10393-018-1374-x
  8. Mesquita, JR; Istrate, C; Santos-Ferreira, NL; Ferreira, AS; Abreu-Silva, J; Veiga, J; van der Poel, WHM; Nascimento, MSJ. "Short communication: detection and molecular characterization of hepatitis E virus in domestic animals of São Tomé and Príncipe". (2018):
    10.1007/s11250-018-1700-x
  9. Cruz, R; Esteves, F; Vasconcelos-Nobrega, C; Santos, C; Ferreira, AS; Mega, C; Coelho, AC; Vala, H; Mesquita, JR. "A Nationwide Seroepidemiologic Study on Q Fever Antibodies in Sheep of Portugal". (2018):
    10.1089/vbz.2018.2294
  10. Cruz, Rita; Esteves, Fernando; Vasconcelos-Nóbrega, Carmen; Santos, Carla; Ferreira, Ana S.; Mega, Cristina; Coelho, Ana C.; Vala, Helena; Mesquita, João R.. "Outbreaks of abortions by Coxiella burnetii in small ruminant flocks and a longitudinal serological approach on archived bulk tank milk suggest Q fever emergence in Central Portugal". Transboundary and Emerging Diseases 65 4 (2018): 972-975. http://dx.doi.org/10.1111/tbed.12913.
    10.1111/tbed.12913
  11. Esteves, F; Cruz, R; Vasconcelos-Nobrega, C; Santos, C; Ferreira, AS; Petrovic, T; Cardoso, L; et al. "Serologic evidence for Schmallenberg virus circulation at high altitude, Central Portugal, 2015-2016". (2018):
    10.1111/tbed.12899
  12. Ferreira, AS; Costa, P; Rocha, T; Amaro, A; Vieira, ML; Ahmed, A; Thompson, G; Hartskeerl, RA; Inácio, J. "Direct Detection and Differentiation of Pathogenic Leptospira Species Using a Multi-Gene Targeted Real Time PCR Approach". (2014):
    10.1371/journal.pone.0112312
  13. Costa, P; Amaro, A; Ferreira, AS; Machado, D; Albuquerque, T; Couto, I; Botelho, A; Viveiros, M; Inácio, J. "Rapid identification of veterinary-relevant Mycobacterium tuberculosis complex species using 16S rDNA, IS6110 and Regions of Difference-targeted dual-labelled hydrolysis probes". (2014):
    10.1016/j.mimet.2014.08.017
  14. Costa, P; Ferreira, AS; Amaro, A; Albuquerque, T; Botelho, A; Couto, I; Cunha, MV; Viveiros, M; Inácio, J. "Enhanced detection of tuberculous mycobacteria in animal tissues using a semi-nested probe-based real-time PCR". (2013):
    10.1371/journal.pone.0081337
  15. Ferreira, AS; Sampaio, A; Maduro, AP; Silva, I; Teles, F; Martins, ML; Inácio, J. "Genotypic diversity of environmental Cryptococcus neoformans isolates from Northern Portugal". (2013):
    10.1111/myc.12106
  16. Martins, ML; Ferreira, AS; Sampaio, A; Vieira, R; Inácio, J. "Direct and specific identification of Cryptococcus neoformans in biological samples using fluorescently-labelled DNA probes". (2010):
    10.1007/s10096-010-0897-z
Thesis / Dissertation
  1. Ferreira, Ana. "Molecular and Serological approaches for the Detection and Typing of Leptospira". PhD, 2015.
  2. Ferreira, Ana. "Diagnóstico laboratorial de micoses humanas – métodos convencionais versus métodos moleculares". Master, Universidade de Trás-os-Montes e Alto Douro Escola de Ciências Agrárias e Veterinárias, 2009.
  3. Ferreira, Ana. "Caracterização de isolados ambientais de Cryptococcus neoformans e sua comparação com isolados clínicos". Degree, Universidade de Trás-os-Montes e Alto Douro Escola de Ciências Agrárias e Veterinárias, 2007.

Other

Research technique
  1. A validated innovative TaqMan-based multi-gene targeted real-time PCR assay was developed for the detection and differentiation of the most relevant pathogenic species of Leptospira in clinical specimens, suitable for being introduced in the routine diagnostics of veterinary laboratories (Ferreira et al. 2014). Researcher. Ferreira, Ana.
    Researcher
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2017/12/11 Q fever surveillance in portuguese sheep: preliminary results European Pathosurveillance Network: 2017 meeting
(Viseu)
2014/04/03 Molecular approaches for the diagnosis of bovine tuberculosis: detection of Mycobacterium bovis by fluorescence in situ hybridization and real time PCR in histological sections. Oral commnunication presented at: Biologia Celular e Molecular VI Congresso da Sociedade Portuguesa de Ciências Veterinárias - CCV2014
(Oeiras, Portugal)
2012/05/31 Design of TaqMan-based PCR assays to distinguish pathogenic Leptospira genomospecies Eurolepto 2012
(Dubrovnik, Croatia)
2011/05/28 Emerging instrument-free diagnostic tools for the molecular detection of zoonotic pathogens XXV Congresso de Iniciação Científica da Unicamp
(Vila Real, Portugal)
2008/10/07 Tinea capitis um problema de saúde pública num bairro suburbano de Lisboa 2ªs Jornadas de Biologia da UTAD
(Vila Real, Portugal)
2007/06/15 Caracterização de isolados ambientais de Cryptococcus neoformans e sua comparação com isolados clínicos XV Jornadas de Biologia de Leveduras "Professor Nicolau Van Uden"
(Porto, Portugal)

Jury of academic degree

Topic
Role 		Candidate name (Type of degree)
Institution / Organization
2021/07/08 Molecular Detection of SARS-CoV-2 from FTA Cards sputum Samples
(Thesis) Main arguer
 Mafalda Castro (Other)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal

Course / Discipline taught

Academic session Degree Subject (Type) Institution / Organization
2018/09/03 - 2018/09/08 O virus da hepatite E nos países industrializados: uma ameaça silenciosa 14th edition of the School of Life Sciences and Health (Ensino secundário) Universidade do Porto, Portugal
2017/09/04 - 2017/09/09 O virus da hepatite E nos países industrializados: uma ameaça silenciosa (Ensino secundário) Universidade do Porto, Portugal
Distinctions

Other distinction

2009 Research Grant (Ref SFRH/BD/62317/2009) to Support Ph.D. in the scope of Veterinary Sciencies
Fundação para a Ciência e a Tecnologia, Portugal