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Identification

Personal identification

Full name
Lígia Maria Oliveira Martins

Citation names

  • Martins, Lígia
  • Martins, Lígia O.

Author identifiers

Ciência ID
731B-B843-C1DF
ORCID iD
0000-0003-0082-9591
Google Scholar ID
https://scholar.google.pt/citations?user=OYWueH0AAAAJ&hl=en
Researcher Id
C-2513-2009
Scopus Author Id
7102109314

Email addresses

  • lmartins@itqb.unl.pt (Professional)

Knowledge fields

  • Engineering and Technology - Industrial Biotechnology - Biocatalysis
  • Engineering and Technology - Environmental Biotechnology - Environmental Biotechnology

Languages

Language Speaking Reading Writing Listening Peer-review
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
French Intermediate (B1) Intermediate (B1) Beginner (A1) Intermediate (B1)
Portuguese (Mother tongue)
Education
Degree Classification
2024/01/18
Concluded
Biociências Moleculares (Título de Agregado)
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
1994
Concluded
PhD in Biotechnology (Doutoramento)
Universidade de Lisboa Instituto Superior Técnico, Portugal
"Via Biossintética dos Nucleótidos de Açúcar Envolvidos na Produção do Exopolissacárido Gelano por Sphingomonas paucimobilis" (THESIS/DISSERTATION)
Distinção e louvor
1990
Concluded
MSc in Biological Engineering/Biotechnology (Mestrado)
Universidade de Lisboa Instituto Superior Técnico, Portugal
"Fisiologia da Biossíntese de Alginato por Pseudomonas aeruginosa: Enzimas Biossintéticas em Variantes Mucosas e Não Mucosas e Efeitos dos Iões Mn2+, Mg2+ e Ca2+" (THESIS/DISSERTATION)
Very Good
1987
Concluded
Degree (“Licenciatura”) in Biology (Licenciatura)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
"Efeito da Temperatura e do Cálcio na Composição em Ácidos Gordos de Bacillus stearothermophilus" (THESIS/DISSERTATION)
16/20
Affiliation

Science

Category
Host institution
Employer
2000/09/01 - 2003/12/31 Auxiliary Researcher (Research) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
1990 - 1991 Visiting Researcher (Research) The University of Edinburgh Institute of Cell Biology, United Kingdom

Teaching in Higher Education

Category
Host institution
Employer
2022/01/26 - Current Associate Professor (University Teacher) Universidade Nova de Lisboa, Portugal
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2014/09/01 - Current Assistant Professor (University Teacher) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2017/06/01 - 2017/06/30 Visiting Professor (University Teacher) Université Claude Bernard Lyon 1, France
2004/01/01 - 2014/08/31 Invited Assistant Professor (University Teacher) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
1998/03/01 - 2005/12/31 Assistant Professor (University Teacher) Universidade Lusófona de Humanidades e Tecnologias, Portugal

Others

Category
Host institution
Employer
2004/10/01 - Current Principal Investigator of the Microbial and Enzyme Technology Laboratory Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2014/07/01 - 2017/06/30 Special Visiting Researcher Fellow Laboratório Nacional de Ciência e Tecnologia do Bioetanol, Brazil
1994/05/01 - 2000/03/31 Post-doc Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
Projects

Grant

Designation Funders
2023 - 2026 Biocat4Suschem: Broadening the spectrum of biocatalysts, bioprocesses and bio-products in industrial biotechnology
FCT 2022.02027.PTDC
Principal investigator
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2022 - 2026 COZYME - Pan-European Network on Computational Redesign of Enzymes
CA21162
Researcher
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
Ongoing
2019 - 2024 B-LigZymes: Bacterial Enzymes and Bioprocesses for Lignin Valorisation
Principal investigator
EU Framework Programme for Research and Innovation Marie Sklodowska-Curie Actions
Ongoing
2022 - 2023 Unravel Structure-Function Relationships for Enhanced Biocatalysts
EXPL/BIA-BQM/0473/2021
Integration into Research Grant Fellow
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2019 - 2023 SMARTBOX: Selective Modifications of Aromatics through Biocatalytic Oxidations
Integration into Research Grant Fellow
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
European Union´s H2020 Research and Innovation Programme
Ongoing
2018 - 2021 FITzymes: Iterative Laboratory-Computational Evolution of Bacterial Ligninolytic Enzymes
PTDC/BII-BBF/29564/2017
Principal investigator
Fundação para a Ciência e a Tecnologia
Concluded
2018 - 2021 ORBioelectronics: Oxidoreductase-based electrochemical biosensors and bioreactors: rational design guided by biophysical characterization
PTDC/BIA-BFS/31026/2017
Integration into Research Grant Fellow
Fundação para a Ciência e a Tecnologia
Concluded
2016 - 2019 Biocatalysis for tackling Lignin Recalcitrance
PTDC/BBB-EBB/0122/2014
Principal investigator
Fundação para a Ciência e a Tecnologia
Concluded
2014 - 2017 Biocatalysis for Tackling Lignin Recalcitrance and Valorisation
Ciência sem Fronteiras: Bolsa de Pesquisante Visitante Especial
Invited Scientist Fellow
Programa Ciência sem Fronteiras
Concluded
2014 - 2015 Discovery and characterization of ligninolytic bacterial enzymes
EXP/BBB-BIO/1932/2013
Integration into Research Grant Fellow
Fundação para a Ciência e a Tecnologia
Concluded
2010/03/01 - 2013 Valorization of the Mediterranean energy crops giant reed and cardoon by integrated bio-chemical conversion to dissolving grade pulps, fuel ethanol, xylitol and lignin-based products - a complex LCF biorefinery concept.
Integration into Research Grant Fellow
Fundação para a Ciência e a Tecnologia
Concluded
2007/09/01 - 2011 Enzymatic Degradation and Synthesis of Azo and Antraquinonic Dyes
Principal investigator
Fundação para a Ciência e a Tecnologia
Concluded
2006 - 2010 White Biotechnology for Added Value Products from Renewable Plant Polymers: Design of Tailor-Made Biocatalysts and New Industrial Processes (BIORENEW)
Integration into Research Grant Fellow
European Commission Sixth Framework Programme
Concluded
2005 - 2008 NanoEngineering of Bacterial Laccases
POCTI/BIO/57083/2004
Principal investigator
Fundação para a Ciência e a Tecnologia
Concluded
2004 - 2008 Novel Sustainable Processes for the European Colour Industries (SOPHIED)
Integration into Research Grant Fellow
European Commission Sixth Framework Programme
Concluded
2006 - 2007 Expression, Purification, Biochemical and Structural Characterization of a Multicopper Oxidase from Myxococcus xanthus
E-67/2006
Principal investigator
Acção Integrada Luso-Espanhola, CRUP
Concluded
Outputs

Publications

Book chapter
  1. Martins, Lígia O.; Melo, Eduardo Pinho; Sanchez-Amat, Antonio; Robalo, Maria Paula. "Bacterial Laccases: Some Recent Advances and Applications". In Laccases in Bioremediation and Waste Valorisation, 27-55. Springer International Publishing, 2020.
    10.1007/978-3-030-47906-0_2
  2. Mendes, Sónia; Robalo, Maria Paula; Martins, Lígia O.. "Bacterial enzymes and multi-enzymatic systems for cleaning-up dyes from the environment". Springer International Publishing, 2015.
    10.1007/978-3-319-10942-8_2
  3. Henriques, A.O.; Costa, T.V; Martins, Lígia O.; Zilhão, R.. "Functional architecture and assembly of the spore coat". In "Bacterial Spore Formers: Probiotics and Emerging applications. London, United Kingdom: Horizon Scientific Press, 2004.
    Published
Conference paper
  1. Brissos, V.; Tavares, D.; Duarte, Z; Lígia O. Martins. "Engineering bacterial ligninolytic enzymes for tackling lignin recalcitrance". Paper presented in 15th European Workshop on Lignocellulosics and Pulp, Aveiro, 2018.
  2. "Improving a bacterial pyranose 2-oxidase using a combination of rational design and directed evolution for biosensor applications". Paper presented in 18th European Congress of Biotechnology, Geneva, 2018.
    Published
  3. "Towards a sustainable production of biologically active building blocks from dye-rich wastewaters". Paper presented in 18th European Congress of Biotechnology, Geneva, 2018.
    Published
  4. Sousa, Ana Catarina; Santos, Iolanda; Martins, Lígia O.; Robalo, Maria Paula. "Biocatalytic synthesis of 1,2-naphtoquinones derivatives mediated by CotA-laccase". 2017.
  5. Sousa, AC.; Martins, Lígia O.; ROBALO, MP. "Laccases: biocatalysts towards new heterocyclic cores". 2016.
  6. Sousa, A.C.; Martins, Lígia O.; Robalo, M.P.. "CotA-laccase as biocatalyst in the “green” synthesis of phenazine and acridine cores". 2016.
  7. Fernandes, AT.; Melo, EP.; Martins, Lígia O.. "Stability studies in the CotA-laccase from Bacillus subtilis and in the metallo-oxidase McoA from Aquifex aeolicus". Paper presented in Oxidative Enzymes as Sustainable Industrial Biocatalysts Workshop, 2010.
  8. Durão, P.; Martins, Lígia O.. "Towards the industrial application of biocatalysts: Elucidation of structural/ functional features of CotA-laccase". Paper presented in 6th International Conference Textile and Polymer Biotechnology, Ghent, 2009.
  9. Durão, P.; Chen, Z.; Pereira, M.M.; Todorovic, S.; Bento, I.; Lindley, P.F.; Martins, Lígia O.. "Evidences for the role of glutamate 498 as a proton donor in the reduction of dioxygen by CotA-laccase". Paper presented in 34th Congress of the Federation of European Biochemical Societies, Prague, 2009.
  10. Chen, Z.; Durão, P.; Fernandes, AT.; Hildebrandt, P.; Murgida, D.H.; Todorovic, S.; Pereira, M.M.; Melo, E.P.; Martins, Lígia O.. "Copper incorporation into recombinant CotA-laccase from Bacillus subtilis: Characterization of fully copper loaded enzymes". Paper presented in 13th International Biotechnology Symposium and Exhibition. “Biotechnology for the Sustainability of Human Society”, Dalían, 2008.
  11. Pereira, L.; Viegas, C.; Coelho, A.; Robalo, M.P.; Martins, Lígia O.. "Mechanisms of enzymatic degradation of azo and anthraquinone dyes by bacterial CotA-laccase". Paper presented in 13th European Congress on Biotechnology, Barcelona, 2007.
  12. Fernandes, AT.; Martins, Lígia O.; Melo, EP.. "Insight into stability of multicopper oxidases". Paper presented in 13th European Congress on Biotechnology, Barcelona, 2007.
  13. Durão, P.; Chen, Z.; Fernandes, AT.; Pereira, MM.; Melo, EP.; Martins, Lígia O.. "Production and characterization of a recombinant Cu-full loaded bacterial laccase". Paper presented in 13th European Congress on Biotechnology, Barcelona, 2007.
  14. Melo, EP.; Fernandes, AT.; Durão, P.; Martins, Lígia O.. "Insight into stability of CotA-laccase from the spore coat of Bacillus subtilis". Paper presented in 7th International Conference on Protein Stabilization, Exeter, 2007.
  15. Henriques, AO.; Martins, Lígia O.; Soares, CM.; Costa, T.; Pereira, MM.; Teixeira, M.. "Studies on CotA, a laccase associated with the surface of Bacillus subtilis endospores". Paper presented in 10th Int Conf on Bioinorganic Chemistry, Florence, 2001.
  16. Sá-Correia, I.; Martins, Lígia O.; Leitão, JH.; Fialho, A.M.; Monteiro, GA.; Lemos, PC.; Rodrigues, PL.. "Manipulation of exopolysaccaride synthesis in Pseudomonas aeruginosa and Pseudomonas elodea". Santiago de Compostela, 1992.
Journal article
  1. Hermann, Eniko; Rodrigues, Carolina F.; Martins, Lígia O.; Peterbauer, Clemens; Oostenbrink, Chris. "Engineering A-type Dye-decolorizing Peroxidases by Modification of a Conserved Glutamate Residue". ChemBioChem (2024): http://dx.doi.org/10.1002/cbic.202300872.
    10.1002/cbic.202300872
  2. Anja Kostelac; André Taborda; Lígia O. Martins; Dietmar Haltrich; Marina Lotti. "Evolution and separation of actinobacterial pyranose and C -glycoside-3-oxidases". Applied and Environmental Microbiology (2024): https://doi.org/10.1128/aem.01676-23.
    10.1128/aem.01676-23
  3. Brissos, Vânia; Borges, Patrícia T.; Sancho, Ferran; Lucas, Maria Fátima; Frazão, Carlos; Conzuelo, Felipe; Martins, Lígia O.. "Flexible active-site loops fine-tune substrate specificity of hyperthermophilic metallo-oxidases". JBIC Journal of Biological Inorganic Chemistry (2024): http://dx.doi.org/10.1007/s00775-023-02040-y.
    10.1007/s00775-023-02040-y
  4. Paulo Durão; Peter Kis; Ivo M. Chelo; M. Rita Ventura; Lígia O. Martins. "Environmentally Friendly Degradation and Detoxification of Rifampicin by a Bacterial Laccase and Hydrogen Peroxide". ChemBioChem (2024): https://doi.org/10.1002/cbic.202300627.
    10.1002/cbic.202300627
  5. Kelly Frade; Célia M. Silveira; Bruno A. Salgueiro; Sónia Mendes; Lígia O. Martins; Carlos Frazão; Smilja Todorovic; Elin Moe. "Biochemical, Biophysical, and Structural Analysis of an Unusual DyP from the Extremophile Deinococcus radiodurans". Molecules (2024): https://doi.org/10.3390/molecules29020358.
    10.3390/molecules29020358
  6. Barbosa, Catarina; Rodrigues, Carolina F.; Loncar, Nikola; Martins, Lígia O.; Todorovic, Smilja; Silveira, Célia M.. "Spectroelectrochemistry for determination of the redox potential in heme enzymes: Dye-decolorizing peroxidases". BBA Advances 5 (2024): 100112. http://dx.doi.org/10.1016/j.bbadva.2023.100112.
    10.1016/j.bbadva.2023.100112
  7. N. Delprat; L.O. Martins; L.J. Blum; C.M.G. Aymard; B. Leca-Bouvier; G. Octobre; B. Doumèche. "User-friendly one-step disposable signal-on bioassay for glyphosate detection in water samples". Biosensors and Bioelectronics (2023): https://doi.org/10.1016/j.bios.2023.115689.
    10.1016/j.bios.2023.115689
  8. Lígia O. Martins. "The quest for new robust bacterial monoamine oxidases". The FEBS Journal (2023): https://doi.org/10.1111/febs.17002.
    10.1111/febs.17002
  9. Taborda, André; Frazão, Tomás; Rodrigues, Miguel V.; Fernández-Luengo, Xavier; Sancho, Ferran; Lucas, Maria Fátima; Frazão, Carlos; et al. "Mechanistic insights into glycoside 3-oxidases involved in C-glycoside metabolism in soil microorganisms". Nature Communications 14 1 (2023): http://dx.doi.org/10.1038/s41467-023-42000-3.
    10.1038/s41467-023-42000-3
  10. Giulia Moro; Vânia Brissos; Chiara Zanardi; Lígia O. Martins; Felipe Conzuelo. "Electrochemical investigations of the multicopper oxidase from Aquifex aeolicus under direct electron transfer with carbon electrodes". Electrochimica Acta (2023): https://doi.org/10.1016/j.electacta.2023.143199.
    10.1016/j.electacta.2023.143199
  11. Maric, Ivana; Guo, Yiming; Fürst, Maximilian J. L. J.; Van Aelst, Korneel; Van den Bosch, Sander; De Simone, Mario; Martins, Lígia O.; Sels, Bert F.; Fraaije, Marco W.. "A One-Pot, Whole-Cell Biocatalysis Approach for Vanillin Production using Lignin Oil". Advanced Synthesis & Catalysis 365 22 (2023): 3987-3995. http://dx.doi.org/10.1002/adsc.202300868.
    10.1002/adsc.202300868
  12. Silva, Diogo; Rodrigues, Carolina F.; Lorena, Constança; Borges, Patrícia T.; Martins, Lígia O.. "Biocatalysis for biorefineries: The case of dye-decolorizing peroxidases". Biotechnology Advances 65 (2023): 108153. http://dx.doi.org/10.1016/j.biotechadv.2023.108153.
    10.1016/j.biotechadv.2023.108153
  13. Magalí F. Scocozza; Francisco Vieyra; Fernando Battaglini; Ligia O. Martins; Daniel H. Murgida. "Electrochemical Actuation of a DyP Peroxidase: A Facile Method for Drastic Improvement of the Catalytic Performance". ACS Catalysis (2023): https://doi.org/10.1021/acscatal.3c01530.
    10.1021/acscatal.3c01530
  14. Silva, Diogo; Sousa, Ana Catarina; Robalo, M. Paula; Martins, Lígia O.. "A wide array of lignin-related phenolics are oxidized by an evolved bacterial dye-decolourising peroxidase". New Biotechnology (2022): http://dx.doi.org/10.1016/j.nbt.2022.12.003.
    10.1016/j.nbt.2022.12.003
  15. Guerra, Vera; Tiago, Igor; Aires, Aitana; Coelho, Catarina; Nunes, João; Martins, Lígia O.; Veríssimo, António. "The gastrointestinal microbiome of browsing goats (Capra hircus)". PLOS ONE 17 10 (2022): e0276262. http://dx.doi.org/10.1371/journal.pone.0276262.
    10.1371/journal.pone.0276262
  16. Mario De Simone; Laura Alvigini; Lur Alonso-Cotchico; Vânia Brissos; Jonatan Caroli; Maria Fátima Lucas; Emanuele Monza; et al. "Rationally Guided Improvement of NOV1 Dioxygenase for the Conversion of Lignin-Derived Isoeugenol to Vanillin". Biochemistry (2022): https://doi.org/10.1021/acs.biochem.2c00168.
    10.1021/acs.biochem.2c00168
  17. Vânia Brissos; Patrícia T. Borges; Reyes Núñez-Franco; Maria Fátima Lucas; Carlos Frazão; Emanuele Monza; Laura Masgrau; Tiago N. Cordeiro; Lígia O. Martins. "Distal Mutations Shape Substrate-Binding Sites during Evolution of a Metallo-Oxidase into a Laccase". ACS Catalysis (2022): https://doi.org/10.1021/acscatal.2c00336.
    10.1021/acscatal.2c00336
  18. Borges, P.T.; Silva, D.; Silva, T.F.D.; Brissos, V.; Cañellas, M.; Lucas, M.F.; Masgrau, L.; et al. "Unveiling molecular details behind improved activity at neutral to alkaline pH of an engineered DyP-type peroxidase". Computational and Structural Biotechnology Journal 20 (2022): 3899-3910. http://www.scopus.com/inward/record.url?eid=2-s2.0-85134879064&partnerID=MN8TOARS.
    10.1016/j.csbj.2022.07.032
  19. Sousa, A.C.; Santos, I.; Piedade, M.F.M.M.; Martins, L.O.; Robalo, M.P.. "Corrigendum to: Synthesis of Substituted 4-Arylamino-1,2-naphthoquinones in One-Pot Reactions Using CotA-Laccase as Biocatalyst (Advanced Synthesis & Catalysis, (2020), 362, 16, (3380-3387), 10.1002/adsc.202000082)". Advanced Synthesis and Catalysis 364 7 (2022): 1346-1346. http://www.scopus.com/inward/record.url?eid=2-s2.0-85127008670&partnerID=MN8TOARS.
    10.1002/adsc.202200255
  20. Magalí F. Scocozza; Lígia O. Martins; Daniel H. Murgida. "Direct Electrochemical Generation of Catalytically Competent Oxyferryl Species of Classes I and P Dye Decolorizing Peroxidases". International Journal of Molecular Sciences (2021): https://doi.org/10.3390/ijms222212532.
    10.3390/ijms222212532
  21. Carolina F. Rodrigues; Patrícia T. Borges; Magali F. Scocozza; Diogo Silva; André Taborda; Vânia Brissos; Carlos Frazão; Lígia O. Martins. "Loops around the Heme Pocket Have a Critical Role in the Function and Stability of BsDyP from Bacillus subtilis". International Journal of Molecular Sciences 22 19 (2021): 10862-10862. https://doi.org/10.3390/ijms221910862.
    10.3390/ijms221910862
  22. Ana Catarina Sousa; Lígia O. Martins; M. Paula Robalo. "Laccases: Versatile Biocatalysts for the Synthesis of Heterocyclic Cores". Molecules 26 12 (2021): 3719-3719. https://doi.org/10.3390/molecules26123719.
    10.3390/molecules26123719
  23. Lange, Lene; Connor, Kevin O.; Arason, Sigurjon; Bundgård-Jørgensen, Uffe; Canalis, Antonella; Carrez, Dirk; Gallagher, Joe; et al. "Developing a Sustainable and Circular Bio-Based Economy in EU: By Partnering Across Sectors, Upscaling and Using New Knowledge Faster, and For the Benefit of Climate, Environment & Biodiversity, and People & Business". Frontiers in Bioengineering and Biotechnology 8 (2021): http://dx.doi.org/10.3389/fbioe.2020.619066.
    Published • 10.3389/fbioe.2020.619066
  24. Fernandes, A.; Pinto, B.; Bonardo, L.; Royo, B.; Robalo, M.P.; Martins, L.O.. "Wasteful Azo Dyes as a Source of Biologically Active Building Blocks". Frontiers in Bioengineering and Biotechnology 9 (2021): http://www.scopus.com/inward/record.url?eid=2-s2.0-85109011577&partnerID=MN8TOARS.
    10.3389/fbioe.2021.672436
  25. Patrícia T. Borges; Vânia Brissos; Guillem Hernandez; Laura Masgrau; Maria Fátima Lucas; Emanuele Monza; Carlos Frazão; Tiago N. Cordeiro; Lígia O. Martins. "Methionine-Rich Loop of Multicopper Oxidase McoA Follows Open-to-Close Transitions with a Role in Enzyme Catalysis". ACS Catalysis (2020): 7162-7176. https://doi.org/10.1021/acscatal.0c01623.
    10.1021/acscatal.0c01623
  26. Robalo, Maria Paula; Martins, Lígia O.; Sousa, Ana Catarina; Santos, Iolanda; Piedade, Maria Fátima M.M.. "Synthesis of Substituted 4-Arylamino-1,2-naphthoquinones in One-Pot Reactions Using CotA-Laccase as Biocatalyst". Advanced Synthesis & Catalysis (2020): http://dx.doi.org/10.1002/adsc.202000082.
    10.1002/adsc.202000082
  27. Barbosa, Catarina; Silveira, Célia M.; Silva, Diogo; Brissos, Vânia; Hildebrandt, Peter; Martins, Lígia O.; Todorovic, Smilja. "Immobilized dye-decolorizing peroxidase (DyP) and directed evolution variants for hydrogen peroxide biosensing". Biosensors and Bioelectronics 153 (2020): 112055.
    10.1016/j.bios.2020.112055
  28. Célia M. Silveira; Elin Moe; Marco Fraaije; Lígia O. Martins; Smilja Todorovic. "Resonance Raman view of the active site architecture in bacterial DyP-type peroxidases". RSC Advances 10 (2020): 11095.
    10.1039/D0RA00950D
  29. Alain R. Puente-Santiago; Daily Rodríguez-Padrón; Xuebo Quan; Mario J. Muñoz Batista; Lígia O. Martins; Sanny Verma; Rajender S. Varma; Jian Zhou; Rafael Luque. "Unprecedented Wiring Efficiency of Sulfonated Graphitic Carbon Nitride Materials: Toward High-Performance Amperometric Recombinant CotA Laccase Biosensors". ACS Sustainable Chemistry & Engineering (2019):
    10.1021/acssuschemeng.8b05107
  30. Sousa, A.C.; Baptista, S.R.; Martins, L.O.; Robalo, M.P.. "Synthesis of Azobenzene Dyes Mediated by CotA Laccase". Chemistry - An Asian Journal 14 1 (2019): 187-193.
    10.1002/asia.201801450
  31. Alba-Molina, David; Rodríguez-Padrón, Daily; Puente-Santiago, Alain R.; Giner-Casares, Juan J.; Martín-Romero, María T.; Camacho, Luis; Martins, Lígia O.; et al. "Mimicking the bioelectrocatalytic function of recombinant CotA laccase through electrostatically self-assembled bioconjugates". Nanoscale 11 4 (2019): 1549-1554.
    10.1039/c8nr06001k
  32. Sousa, A.C.; Conceição Oliveira, M.; Martins, L.O.; Robalo, M.P.. "A Sustainable Synthesis of Asymmetric Phenazines and Phenoxazinones Mediated by CotA-Laccase". Advanced Synthesis and Catalysis 360 3 (2018): 575-583.
    10.1002/adsc.201701228
  33. Blánquez, A.; Rodríguez, J.; Brissos, V.; Mendes, S.; Martins, L.O.; Ball, A.S.; Arias, M.E.; Hernández, M.. "Decolorization and detoxification of textile dyes using a versatile Streptomyces laccase-natural mediator system". Saudi Journal of Biological Sciences 26 (2018): 913-920.
    10.1016/j.sjbs.2018.05.020
  34. Brissos, V.; Tavares, D.; Sousa, A.C.; Robalo, M.P.; Martins, L.O.. "Engineering a Bacterial DyP-Type Peroxidase for Enhanced Oxidation of Lignin-Related Phenolics at Alkaline pH". ACS Catalysis 7 5 (2017): 3454-3465.
    10.1021/acscatal.6b03331
  35. Gonçalves, I.; Nunes, C.; Mendes, S.; Martins, L.O.; Ferreira, P.; Coimbra, M.A.. "CotA laccase-ABTS/hydrogen peroxide system: An efficient approach to produce active and decolorized chitosan-genipin films". Carbohydrate Polymers 175 (2017): 628-635.
    10.1016/j.carbpol.2017.08.029
  36. Pistone, L.; Ottolina, G.; De, S.; Romero, A.A.; Martins, L.O.; Luque, R.. "Encapsulated Laccases for the Room-Temperature Oxidation of Aromatics: Towards Synthetic Low-Molecular-Weight Lignins". ChemSusChem 9 7 (2016): 756-762.
    10.1002/cssc.201501427
  37. Sousa, Ana Catarina; Piedade, M. Fatima M. M.; Martins, Ligia O.; Paula Robalo, M.. "Eco-friendly synthesis of indo dyes mediated by a bacterial laccase". Green Chemistry 18 22 (2016): 6063-6070.
    10.1039/c6gc02050j
  38. Mendes, S.; Banha, C.; Madeira, J.; Santos, D.; Miranda, V.; Manzanera, M.; Ventura, M.R.; van Berkel, W.J.H.; Martins, L.O.. "Characterization of a bacterial pyranose 2-oxidase from Arthrobacter siccitolerans". Journal of Molecular Catalysis B: Enzymatic 133 (2016): S34-S43.
    10.1016/j.molcatb.2016.11.005
  39. Brissos, V.; Ferreira, M.; Grass, G.; Martins, L.O.. "Turning a Hyperthermostable Metallo-Oxidase into a Laccase by Directed Evolution". ACS Catalysis 5 8 (2015): 4932-4941.
    10.1021/acscatal.5b00771
  40. Martins, Ligia O.; Durao, Paulo; Brissos, Vania; Lindley, Peter F.. "Laccases of prokaryotic origin: enzymes at the interface of protein science and protein technology". Cellular and Molecular Life Sciences 72 5 (2015): 911-922.
    10.1007/s00018-014-1822-x
  41. Sousa, A.C.; Piedade, M.F.M.M.; Martins, L.O.; Robalo, M.P.. "An enzymatic route to a benzocarbazole framework using bacterial CotA laccase". Green Chemistry 17 3 (2015): 1429-1433.
    10.1039/c4gc02511c
  42. Mendes, S.; Brissos, V.; Gabriel, A.; Catarino, T.; Turner, D.L.; Todorovic, S.; Martins, L.O.. "An integrated view of redox and catalytic properties of B-type PpDyP from Pseudomonas putida MET94 and its distal variants". Archives of Biochemistry and Biophysics 574 (2015): 99-107.
    10.1016/j.abb.2015.03.009
  43. Todorovic, S.; Hildebrandt, P.; Martins, L.O.. "Surface enhanced resonance Raman detection of a catalytic intermediate of DyP-type peroxidase". Physical Chemistry Chemical Physics 17 18 (2015): 11954-11957.
    10.1039/c5cp01283j
  44. Mendes, S.; Catarino, T.; Silveira, C.; Todorovic, S.; Martins, L.O.. "The catalytic mechanism of A-type dye-decolourising peroxidase BsDyP: neither aspartate nor arginine is individually essential for peroxidase activity". Catalysis Science and Technology 5 12 (2015): 5196-5207.
    10.1039/c5cy00478k
  45. Santos, Ana; Mendes, Sonia; Brissos, Vania; Martins, Ligia O.. "New dye-decolorizing peroxidases from Bacillus subtilis and Pseudomonas putida MET94: towards biotechnological applications". Applied Microbiology and Biotechnology 98 5 (2014): 2053-2065.
    10.1007/s00253-013-5041-4
  46. Lang, W.; Sirisansaneeyakul, S.; Martins, L.O.; Ngiwsara, L.; Sakairi, N.; Pathom-aree, W.; Okuyama, M.; Mori, H.; Kimura, A.. "Biodecolorization of a food azo dye by the deep sea Dermacoccus abyssi MT1.1 strain from the Mariana Trench". Journal of Environmental Management 132 (2014): 155-164.
    10.1016/j.jenvman.2013.11.002
  47. Brissos, V.; Gonçalves, N.; Melo, E.P.; Martins, L.O.. "Improving kinetic or thermodynamic stability of an azoreductase by directed evolution". PLoS ONE 9 1 (2014):
    10.1371/journal.pone.0087209
  48. Sousa, A.C.; Oliveira, M.C.; Martins, L.O.; Robalo, M.P.. "Towards the rational biosynthesis of substituted phenazines and phenoxazinones by laccases". Green Chemistry 16 9 (2014): 4127-4136.
    10.1039/c4gc00901k
  49. Lang, W.; Sirisansaneeyakul, S.; Ngiwsara, L.; Mendes, S.; Martins, L.O.; Okuyama, M.; Kimura, A.. "Characterization of a new oxygen-insensitive azoreductase from Brevibacillus laterosporus TISTR1911: Toward dye decolorization using a packed-bed metal affinity reactor". Bioresource Technology 150 (2013): 298-306.
    10.1016/j.biortech.2013.09.124
  50. Sezer, M.; Santos, A.; Kielb, P.; Pinto, T.; Martins, L.O.; Todorovic, S.. "Distinct structural and redox properties of the heme active site in bacterial dye decolorizing peroxidase-type peroxidases from two subfamilies: Resonance raman and electrochemical study". Biochemistry 52 18 (2013): 3074-3084.
    10.1021/bi301630a
  51. Goncalves, Ana Maria D.; Mendes, Sonia; de Sanctis, Daniele; Martins, Ligia O.; Bento, Isabel. "The crystal structure of Pseudomonas putida azoreductase - the active site revisited". Febs Journal 280 24 (2013): 6643-6657.
    10.1111/febs.12568
  52. Sousa, A.C.; Martins, L.O.; Robalo, M.P.. "Laccase-catalysed homocoupling of primary aromatic amines towards the biosynthesis of dyes". Advanced Synthesis and Catalysis 355 14-15 (2013): 2908-2917.
    10.1002/adsc.201300501
  53. Rosado, T.; Bernardo, P.; Koci, K.; Coelho, A.V.; Robalo, M.P.; Martins, L.O.. "Methyl syringate: An efficient phenolic mediator for bacterial and fungal laccases". Bioresource Technology 124 (2012): 371-378.
    10.1016/j.biortech.2012.08.023
  54. Fernandes, A.T.; Lopes, C.; Martins, L.O.; Melo, E.P.. "Unfolding pathway of CotA-laccase and the role of copper on the prevention of refolding through aggregation of the unfolded state". Biochemical and Biophysical Research Communications 422 3 (2012): 442-446.
    10.1016/j.bbrc.2012.05.011
  55. Silva, Catarina S.; Durao, Paulo; Fillat, Amanda; Lindley, Peter F.; Martins, Ligia O.; Bento, Isabel. "Crystal structure of the multicopper oxidase from the pathogenic bacterium Campylobacter jejuni CGUG11284: characterization of a metallo-oxidase". Metallomics 4 1 (2012): 37-47.
    10.1039/c1mt00156f
  56. Sezer, Murat; Genebra, Tania; Mendes, Sonia; Martins, Ligia O.; Todorovic, Smilja. "A DyP-type peroxidase at a bio-compatible interface: structural and mechanistic insights". Soft Matter 8 40 (2012): 10314-10321.
    10.1039/c2sm26310f
  57. Silva, Catarina S.; Damas, Joao M.; Chen, Zhenjia; Brissos, Vania; Martins, Ligia O.; Soares, Claudio M.; Lindley, Peter F.; Bento, Isabel. "The role of Asp116 in the reductive cleavage of dioxygen to water in CotA laccase: assistance during the proton-transfer mechanism". Acta Crystallographica Section D-Biological Crystallography 68 (2012): 186-193.
    10.1107/S0907444911054503
  58. Brissos, Vania; Chen, Zhenjia; Martins, Ligia O.. "The kinetic role of carboxylate residues in the proximity of the trinuclear centre in the O-2 reactivity of CotA-laccase". Dalton Transactions 41 20 (2012): 6247-6255.
    10.1039/c2dt12067d
  59. Correia, B.; Chen, Z.; Mendes, S.; Martins, L.O.; Bento, I.. "Crystallization and preliminary X-ray diffraction analysis of the azoreductase PpAzoR from Pseudomonas putida MET94". Acta Crystallographica Section F: Structural Biology and Crystallization Communications 67 1 (2011): 121-123.
    10.1107/S1744309110048220
  60. Mendes, S.; Pereira, L.; Batista, C.; Martins, L.O.. "Molecular determinants of azo reduction activity in the strain Pseudomonas putida MET94". Applied Microbiology and Biotechnology 92 2 (2011): 393-405.
    10.1007/s00253-011-3366-4
  61. Mendes, S.; Farinha, A.; Ramos, C.G.; Leitão, J.H.; Viegas, C.A.; Martins, L.O.. "Synergistic action of azoreductase and laccase leads to maximal decolourization and detoxification of model dye-containing wastewaters". Bioresource Technology 102 21 (2011): 9852-9859.
    10.1016/j.biortech.2011.07.108
  62. Fernandes, A.T.; Pereira, M.M.; Silva, C.S.; Lindley, P.F.; Bento, I.; Melo, E.P.; Martins, L.O.. "The removal of a disulfide bridge in CotA-laccase changes the slower motion dynamics involved in copper binding but has no effect on the thermodynamic stability". Journal of Biological Inorganic Chemistry 16 4 (2011): 641-651.
    10.1007/s00775-011-0768-9
  63. Bento, I.; Silva, C.S.; Chen, Z.; Martins, L.O.; Lindley, P.F.; Soares, C.M.. "Mechanisms underlying dioxygen reduction in laccases. Structural and modelling studies focusing on proton transfer". BMC Structural Biology 10 (2010):
    10.1186/1472-6807-10-28
  64. Fernandes, A.T.; Damas, J.M.; Todorovic, S.; Huber, R.; Baratto, M.C.; Pogni, R.; Soares, C.M.; Martins, L.O.. "The multicopper oxidase from the archaeon Pyrobaculum aerophilum shows nitrous oxide reductase activity". FEBS Journal 277 15 (2010): 3176-3189.
    10.1111/j.1742-4658.2010.07725.x
  65. Chen, Zhenjia; Durão, Paulo; Silva, Catarina S.; Pereira, Manuela M.; Todorovic, Smilja; Hildebrandt, Peter; Bento, Isabel; Lindley, Peter F.; Martins, Lígia O.. "The role of Glu498 in the dioxygen reactivity of CotA-laccase from Bacillus subtilis". Dalton Transactions 39 11 (2010): 2875.
    10.1039/b922734b
  66. Fernandes, A.T.; Martins, L.O.; Melo, E.P.. "The hyperthermophilic nature of the metallo-oxidase from Aquifex aeolicus". Biochimica et Biophysica Acta - Proteins and Proteomics 1794 1 (2009): 75-83.
    10.1016/j.bbapap.2008.09.006
  67. Brissos, V.; Pereira, L.; Munteanu, F.-D.; Cavaco-Paulo, A.; Martins, L.O.. "Expression system of CotA-laccase for directed evolution and high-throughput screenings for the oxidation of high-redox potential dyes". Biotechnology Journal 4 4 (2009): 558-563.
    10.1002/biot.200800248
  68. Pereira, L.; Coelho, A.V.; Viegas, C.A.; Ganachaud, C.; Iacazio, G.; Tron, T.; Robalo, M.P.; Martins, L.O.. "On the mechanism of biotransformation of the anthraquinonic dye acid blue 62 by lacases". Advanced Synthesis and Catalysis 351 11-12 (2009): 1857-1865.
    10.1002/adsc.200900271
  69. PEREIRA, L; COELHO, AV; VIEGAS, CA; DOS SANTOS, MMC; ROBALO, MP; MARTINS, LO. "Enzymatic biotransformation of the azo dye Sudan Orange G with bacterial CotA-laccase". Journal of Biotechnology 139 1 (2009): 68-77.
    10.1016/j.jbiotec.2008.09.001
  70. Chen, Z.; Durao, P.; Fernandes, A. T.; Martins, L. O.; Melo, E. P.; Pereira, M.. "Production and characterization of a recombinant Cu-full loaded bacterial laccase (vol 131, pg S113, 2007)". Journal of Biotechnology 144 4 (2009): 341-341.
    10.1016/j.jbiotec.2009.09.018
  71. DURAO, P; CHEN, ZJ; SILVA, CS; SOARES, CM; PEREIRA, MM; TODOROVIC, S; HILDEBRANDT, P; et al. "Proximal mutations at the type 1 copper site of CotA laccase: spectroscopic, redox, kinetic and structural characterization of I494A and L386A mutants". BIOCHEMICAL JOURNAL 412 (2008): 339-346.
    10.1042/BJ20080166
  72. DURAO, P; CHEN, Z; FERNANDES, AT; HILDEBRANDT, P; MURGIDA, DH; TODOROVIC, S; PEREIRA, MM; MELO, EP; MARTINS, LO. "Copper incorporation into recombinant CotA laccase from Bacillus subtilis: characterization of fully copper loaded enzymes". JBIC Journal of Biological Inorganic Chemistry 13 2 (2008): 183-193.
    10.1007/s00775-007-0312-0
  73. Sánchez-Sutil, M.C.; Gómez-Santos, N.; Moraleda-Muñoz, A.; Martins, L.O.; Pérez, J.; Muñoz-Dorado, J.. "Differential expression of the three multicopper oxidases from Myxococcus xanthus". Journal of Bacteriology 189 13 (2007): 4887-4898.
    10.1128/JB.00309-07
  74. Melo, E.P.; Fernandes, A.T.; Durão, P.; Martins, L.O.. "Insight into stability of CotA laccase from the spore coat of Bacillus subtilis". Biochemical Society Transactions 35 6 (2007): 1579-1582.
    10.1042/BST0351579
  75. Fernandes, A.T.; Soares, C.M.; Pereira, M.M.; Huber, R.; Grass, G.; Martins, L.O.. "A robust metallo-oxidase from the hyperthermophilic bacterium Aquifex aeolicus". FEBS Journal 274 11 (2007): 2683-2694.
    10.1111/j.1742-4658.2007.05803.x
  76. DURAO, P; BENTO, I; FERNANDES, AT; MELO, EP; LINDLEY, PF; MARTINS, LO. "Perturbations of the T1 copper site in the CotA laccase from Bacillus subtilis: structural, biochemical, enzymatic and stability studies". JBIC Journal of Biological Inorganic Chemistry 11 4 (2006): 514-526.
    10.1007/s00775-006-0102-0
  77. Lameiro, M.H.; Lopes, A.; Martins, L.O.; Alves, P.M.; Melo, E.. "Incorporation of a model protein into chitosan-bile salt microparticles". International Journal of Pharmaceutics 312 1-2 (2006): 119-130.
    10.1016/j.ijpharm.2006.01.006
  78. Lamosa, P.; Gonçalves, L.G.; Rodrigues, M.V.; Martins, L.O.; Raven, N.D.H.; Santos, H.. "Occurrence of 1-glyceryl-1-myo-inosityl phosphate in hyperthermophiles". Applied and Environmental Microbiology 72 9 (2006): 6169-6173.
    10.1128/AEM.00852-06
  79. Zilhão, R.; Isticato, R.; Martins, L.O.; Steil, L.; Völker, U.; Ricca, E.; Moran Jr., C.P.; Henriques, A.O.. "Assembly and function of a spore coat-associated transglutaminase of Bacillus subtilis". Journal of Bacteriology 187 22 (2005): 7753-7764.
    10.1128/JB.187.22.7753-7764.2005
  80. Bento, I.; Martins, L.O.; Lopes, G.G.; Carrondo, M.A.; Lindley, P.F.. "Dioxygen reduction by multi-copper oxidases; a structural perspective". Dalton Transactions 21 (2005): 3507-3513.
    10.1039/b504806k
  81. Costa, T.; Steil, L.; Martins, L.O.; Völker, U.; Henriques, A.O.. "Assembly of An Oxalate Decarboxylase Produced under sK Control into the Bacillus subtilis Spore Coat". Journal of Bacteriology 186 5 (2004): 1462-1474.
    10.1128/JB.186.5.1462-1474.2004
  82. ENGUITA, FJ; MARCAL, D; MARTINS, LO; GRENHA, R; HENRIQUES, AO; LINDLEY, PF; CARRONDO, MA. "Substrate and doxygen binding to the endospore coat laccase from Bacillus subtilis". JOURNAL OF BIOLOGICAL CHEMISTRY 279 22 (2004): 23472-23476.
    10.1074/jbc.M314000200
  83. Enguita, F.J.; Martins, L.O.; Henriques, A.O.; Carrondo, M.A.. "Crystal structure of a bacterial endospore coat component: A laccase with enhanced thermostability properties". Journal of Biological Chemistry 278 21 (2003): 19416-19425.
    10.1074/jbc.M301251200
  84. Martins, L.O.; Soares, C.M.; Pereira, M.M.; Teixeira, M.; Costa, T.; Jones, G.H.; Henriques, A.O.. "Molecular and biochemical characterization of a highly stable bacterial laccase that occurs as a structural component of the Bacillus subtilis endospore coat". Journal of Biological Chemistry 277 21 (2002): 18849-18859.
    10.1074/jbc.M200827200
  85. Enguita, F.J.; Matias, P.M.; Martins, L.O.; Plácido, D.; Henriques, A.O.; Carrondo, M.A.. "Spore-coat laccase CotA from Bacillus subtilis: Crystallization and preliminary X-ray characterization by the MAD method". Acta Crystallographica Section D: Biological Crystallography 58 9 (2002): 1490-1493.
    10.1107/S0907444902011575
  86. Melo, E.P.; Faria, T.Q.; Martins, L.O.; Gonalves, A.M.; Cabral, J.M.S.. "Cutinase unfolding and stabilization by trehalose and mannosylglycerate". Proteins: Structure, Function and Genetics 42 4 (2001): 542-552.
    10.1002/1097-0134(20010301)42:4<542::AID-PROT120>3.0.CO;2-4
  87. Fialho, A.M.; Martins, L.O.; Donval, M.-L.; Leitão, J.H.; Ridout, M.J.; Jay, A.J.; Morris, V.J.; Sá-Correia, I.. "Structures and properties of gellan polymers produced by Sphingomonas paucimobilis ATCC 31461 from lactose compared with those produced from glucose and from cheese whey". Applied and Environmental Microbiology 65 6 (1999): 2485-2491.
  88. Martins, L.O.; Empadinhas, N.; Marugg, J.D.; Miguel, C.; Ferreira, C.; Da Costa, M.S.; Santos, H.. "Biosynthesis of mannosylglycerate in the thermophilic bacterium Rhodothermus marinus. Biochemical and genetic characterization of a mannosylglycerate synthase". Journal of Biological Chemistry 274 50 (1999): 35407-35414.
    10.1074/jbc.274.50.35407
  89. Silva, Z.; Borges, N.; Martins, L.O.; Wait, R.; Da Costa, M.S.; Santos, H.. "Combined effect of the growth temperature and salinity of the medium on the accumulation of compatible solutes by Rhodothermus marinus and Rhodothermus obamensis". Extremophiles 3 2 (1999): 163-172.
    10.1007/s007920050112
  90. Lamosa, P.; Martins, L.O.; Da Costa, M.S.; Santos, H.. "Effects of temperature, salinity, and medium composition on compatible solute accumulation by Thermococcus spp.". Applied and Environmental Microbiology 64 10 (1998): 3591-3598.
  91. Martins, L.O.; Huber, R.; Huber, H.; Stetter, K.O.; Da Costa, M.S.; Santos, H.. "Organic solutes in hyperthermophilic Archaea". Applied and Environmental Microbiology 63 3 (1997): 896-902.
  92. Martins, L.O.; Fialho, A.M.; Rodrigues, P.L.; Sá-Correia, I.. "Gellan gum production and activity of biosynthetic enzymes in Sphingomonas paucimobilis mucoid and non-mucoid variants". Biotechnology and Applied Biochemistry 24 1 (1996): 47-54.
  93. Xavier, K.B.; Martins, L.O.; Peist, R.; Kossmann, M.; Boos, W.; Santos, H.. "High-affinity maltose/trehalose transport system in the hyperthermophilic archaeon Thermococcus litoralis". Journal of Bacteriology 178 16 (1996): 4773-4777.
    10.1128/jb.178.16.4773-4777.1996
  94. Martins, L.O.; Carreto, L.S.; Da Costa, M.S.; Santos, H.. "New compatible solutes related to di-myo-inositol-phosphate in members of the order Thermotogales". Journal of Bacteriology 178 19 (1996): 5644-5651.
    10.1128/jb.178.19.5644-5651.1996
  95. Martins, L.O.; Santos, H.. "Accumulation of mannosylglycerate and Di-myo-inositol-phosphate by Pyrococcus furiosus in response to salinity and temperature". Applied and Environmental Microbiology 61 9 (1995): 3299-3303.
  96. Martins, L.O.; Sá-Correia, I.. "Temperature profiles of gellan gum synthesis and activities of biosynthetic enzymes". Biotechnology and Applied Biochemistry 20 3 (1994): 385-395. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028189126&partnerID=MN8TOARS.
    10.1111/j.1470-8744.1994.tb00324.x
  97. Martins, L.O.; Sá-Correia, I.. "Alginate biosynthesis in mucoid recombinants of Pseudomonas aeruginosa overproducing GDP-mannose dehydrogenase". Enzyme and Microbial Technology 13 5 (1991): 385-389.
    10.1016/0141-0229(91)90199-K
  98. Alves, M.J.; Martins, L.O.; Sá-Correia, I.. "Temperature profiles and alginate synthesis in mucoid and non-mucoid variants of Pseudomonas aeruginosa". Letters in Applied Microbiology 12 6 (1991): 244-248.
    10.1111/j.1472-765X.1991.tb00550.x
  99. Martins, L.O.; Sá-Correia, I.. "Gellan gum biosynthetic enzymes in producing and nonproducing variants of Pseudomonas elodea.". Biotechnology and applied biochemistry 14 3 (1991): 357-364.
  100. Martins, L.O.; Brito, L.C.; Sá-Correia, I.. "Roles of Mn2+, Mg2+ and Ca2+ on alginate biosynthesis by Pseudomonas aeruginosa". Enzyme and Microbial Technology 12 10 (1990): 794-799.
    10.1016/0141-0229(90)90154-I
  101. Martins, Ligia O.; Brito, Luisa C.; Sa-Correia, Isabel. "Roles of Mn2+, Mg2+ and Ca2+ on alginate biosynthesis by Pseudomonas aeruginosa". Integration, the VLSI Journal 9 3 (1990): 794-799.
  102. Martins, L.O.; Jurado, A.S.; Madeira, V.M.C.. "Composition of polar lipid acyl chains of Bacillus stearothermophilus as affected by temperature and calcium". Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism 1045 1 (1990): 17-20.
    10.1016/0005-2760(90)90198-7
Preprint
  1. Ligia O Martins. "Mechanistic Insights into Glycoside 3-Oxidases Involved in C-Glycoside Metabolism in Soil Microorganisms". 2023. https://doi.org/10.21203/rs.3.rs-2662172/v1.
    10.21203/rs.3.rs-2662172/v1
Thesis / Dissertation
  1. Taborda, André Filipe Teixeira. "Development of novel bacterial enzymes for medical diagnosis through directed evolution". Master, Universidade de Lisboa Faculdade de Ciências, 2019. http://hdl.handle.net/10451/40381.
  2. Fernandes, André João Tavares. "Insight into the multicopper oxidases stability". PhD, 2011. http://hdl.handle.net/10362/8562.