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André R.A. Marques graduated in Biochemistry (BSc, University of Lisbon; MSc, University of Amsterdam) and currently works as Assistant Researcher at the NOVA Medical School (NMS, Portugal). He obtained his Ph.D. in Medical Biochemistry at the University of Amsterdam, The Netherlands. During his Ph.D. studies he investigated the role of lysosomal homeostasis in neurodegeneration. Afterwards, André developed a 3-year PostDoctoral Project at the University of Kiel (Germany), centred on Batten disease (NCL2TREAT Consortium), the most common inherited neurodegenerative disease in children. This project resulted in the registration of an international patent for the treatment of Batten disease, of which he is co-inventor. Since 2019, A. Marques has been developing a project investigating the role of lysosomal dysfunction in atherosclerosis at the NMS. Last year, he was awarded a position as Assistant Researcher by the Portuguese Foundation for Science and Technology (FCT), to develop a 6-year project focused on the interplay between lipid metabolism and organelle dysfunction in human disease. During the course of his career, A. R. A. Marques has published 31 scientific articles (h index = 18, Citations = 1181, Source = Scopus) and was awarded two research grants from the FCT. He has also participated in numerous international scientific conferences and workshops, having been selected to present his work on four occasions, including at a Gordon Research Conference. Furthermore, he has supervised several BSc, MSc and PhD students, and a M.D. doctoral thesis.
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Identification

Personal identification

Full name
André Rosa Alcalde Marques

Citation names

  • Marques, André R. A.

Author identifiers

Ciência ID
7512-8D9D-138D
ORCID iD
0000-0001-9674-3017

Email addresses

  • andre.marques@nms.unl.pt (Professional)

Knowledge fields

  • Natural sciences - Biological Sciences - Biochemistry
Education
Degree Classification
2011/09/01 - 2016/06/08
Concluded
 Medical Biochemistry (Doctor of Philosophy)
Academisch Medisch Centrum Faculteit Geneeskunde, Netherlands

Universiteit van Amsterdam, Netherlands
"Lysosomal glycosidases and glycosphingolipids: new avenues for research" (THESIS/DISSERTATION)
2009/09/01 - 2011/07/29
Concluded
 Medical Biochemistry (Master)
Universiteit van Amsterdam Faculteit der Natuurwetenschappen Wiskunde en Informatica, Netherlands
"Detection of glucocerebrosidase with fluorescent suicide inhibitors " (THESIS/DISSERTATION)
 8.5/10 Cum laude
2006/09/01 - 2009/07/15
Concluded
 Bioquímica (Licenciatura)
Universidade de Lisboa Faculdade de Ciências, Portugal
 17/20
Affiliation

Science

Category
Host institution 		Employer
2025/02/01 - Current Auxiliary Researcher (Research) Universidade Nova de Lisboa Faculdade de Ciências Médicas, Portugal
Universidade Nova de Lisboa Medical School, Portugal
2019/03/01 - 2025/01/31 Contracted Researcher (Research) Universidade Nova de Lisboa Faculdade de Ciências Médicas, Portugal
Universidade Nova de Lisboa Faculdade de Ciências Médicas, Portugal
2016/03/01 - 2018/01/31 Postdoc (Research) Christian-Albrechts-Universität zu Kiel Biochemisches Institut, Germany
Christian-Albrechts-Universität zu Kiel Biochemisches Institut, Germany
2011/09/01 - 2016/02/29 Researcher (Research) Academisch Medisch Centrum Faculteit Geneeskunde, Netherlands
Academisch Medisch Centrum Faculteit Geneeskunde, Netherlands
2010/09/01 - 2011/05/31 Research Trainee (Research) Academisch Medisch Centrum Faculteit Geneeskunde, Netherlands
Academisch Medisch Centrum Faculteit Geneeskunde, Netherlands
2009/09/01 - 2010/06/30 Research Trainee (Research) Universiteit van Amsterdam Swammerdam Institute for Life Sciences, Netherlands
Universiteit van Amsterdam Swammerdam Institute for Life Sciences, Netherlands
2008/12/01 - 2009/08/31 Research Trainee (Research) Universidade de Lisboa Departamento de Química e Bioquímica, Portugal
Universidade de Lisboa Departamento de Química e Bioquímica, Portugal
Outputs

Publications

Conference poster
  1. Guerreiro Simões, Catarina; Lopes, Elizeth; Ferreira, Inês S.; Ribeiro, Quélia; Ramalho, José; Marques, André R. A.; Vieira, Otília V. Corresponding author: Vieira, Otília V. "Unravelling the Connection Between Lysosomal Dysfunction and Senescence in Atherogenesis". Paper presented in NMS PhD Retreat 2024 - Lisbon, 2024.
  2. Inês S. Ferreira; Ribeiro, Quélia; Elizeth Lopes; Guerreiro Simões, Catarina; Hall, Michael J; Ramalho, Jose; Marques, André R. A.; Otilia V. Vieira. "Exploring the Effects of Oxidized Lipids on Murine Macrophages: A Pathway to Atherosclerosis". Paper presented in International FOR2625 Symposium on LYSOSOMES & AUTOPHAGY, 2024.
  3. Inês S. Ferreira; Ribeiro, Quélia; Elizeth Lopes; Guerreiro Simões, Catarina; Hall, Michael J; Ramalho, Jose; Marques, André R. A.; Vieira, Otília V. "Unravelling Atherosclerosis: Exploring Lysosomal Dysfunction and Cell Death in Murine Macrophages Exposed to Oxidized Lipids". Paper presented in 2nd MIA-Portugal Ageing Spring School, 2024.
  4. Marques, André R. A.; Ferraz, Maria J.; Ferreira, Inês S.; Lopes, Elizeth; Guerreiro Simões, Catarina; Aerts, Johannes M. F. G.; Vieira, Otília V. "Atherosclerotic plaques accumulate glucosylated cholesterol in response to lysosomal glycosphingolipid and cholesterol storage". Paper presented in Glycolipid and Sphingolipid Biology Gordon Research Conference, 2024.
  5. Luís Fialho; Inês S. Ferreira; Guerreiro Simões, Catarina; Lopes, Elizeth; Marques, André R. A.; Ramalho, José; Vieira, Otília V. "Cellular and Lysosomal Dysfunction Induced In Vitro by Cholesteryl Hemiglutarate in RAW264.7 Cells". Paper presented in Programa de Integração de Alunos em Trabalhos de Investigação (PIATI), 2023.
  6. Lopes, Elizeth; Machado-Oliveira, Gisela; Guerreiro Simões, Catarina; Inês S. Ferreira; Ramos, Cristiano; Ramalho, José; Maria I. L. Soares; et al. "The pro-atherogenic lipid cholesteryl hemiazelate induces lysosome dysfunction in murine fibroblasts". Paper presented in 1st NMS Research Week, 2023.
  7. Inês S. Ferreira; Lopes, Elizeth; Guerreiro Simões, Catarina; Marques, André R. A.; Vieira, Otília V.. "The Impact of Oxidized Lipids in the Homeostasis of Murine Macrophages: Insights into Atherosclerosis". Paper presented in 1st NMS Research Week, 2023.
  8. L. Alves; Marques, André R. A.; N. Padrão; F.A. Carvalho; C. Lopes; Maria I. L. Soares; Teresa M. V. D. Pinho e Melo; N. C. Santos; O. V. Vieira. "Lysosomal dysfunction in vascular smooth muscle cells plays a role in atherosclerosis". Paper presented in Workshop on Lysosome Biology - 2019 EDITION, 2019.
Journal article
  1. Elizeth Lopes; Gisela Machado-Oliveira; Catarina Guerreiro Simões; Inês S. Ferreira; Cristiano Ramos; José Ramalho; Maria I. L. Soares; et al. "Cholesteryl Hemiazelate Present in Cardiovascular Disease Patients Causes Lysosome Dysfunction in Murine Fibroblasts". Cells (2023): https://doi.org/10.3390/cells12242826.
    10.3390/cells12242826
  2. Neuza Domingues; Joana Gaifem; Rune Matthiesen; Diana P. Saraiva; Luís Bento; André R.A. Marques; Maria I.L. Soares; et al. "Cholesteryl hemiazelate identified in CVD patients causes in vitro and in vivo inflammation". Journal of Lipid Research (2023): https://doi.org/10.1016/j.jlr.2023.100419.
    10.1016/j.jlr.2023.100419
  3. Neuza Domingues; Marques, André R. A.; Rita Diogo Almeida Calado; Inês S. Ferreira; Cristiano Ramos; José Ramalho; Maria I. L. Soares; et al. Corresponding author: Otília V. Vieira. "Oxidized cholesteryl ester induces exocytosis of dysfunctional lysosomes in lipidotic macrophages". Traffic (2023): https://doi.org/10.1111/tra.12888.
    Published • 10.1111/tra.12888
  4. Yen-Hsi Chen; Weihua Tian; Makiko Yasuda; Zilu Ye; Ming Song; Ulla Mandel; Claus Kristensen; et al. "A universal GlycoDesign for lysosomal replacement enzymes to improve circulation time and biodistribution". Frontiers in Bioengineering and Biotechnology 11 (2023): http://dx.doi.org/10.3389/fbioe.2023.1128371.
    Published • 10.3389/fbioe.2023.1128371
  5. Chi-Lin Kuo; Qin Su; Adrianus M. C. H. van den Nieuwendijk; Thomas J.M. Beenakker; Wendy A. Offen; Lianne Irene Willems; Rolf. G. Boot; et al. "The development of a broad-spectrum retaining ß-exo-galactosidase activity-based probe". Organic & Biomolecular Chemistry (2023): http://dx.doi.org/10.1039/d3ob01261a.
    10.1039/d3ob01261a
  6. Lisa Gallwitz; Lina Schmidt; André R. A. Marques; Andreas Tholey; Liam Cassidy; Irem Ulku; Gerhard Multhaup; Alessandro Di Spiezio; Paul Saftig. "Cathepsin D: Analysis of its potential role as an amyloid beta degrading protease". Neurobiology of Disease (2022): 105919-105919. http://dx.doi.org/10.1016/j.nbd.2022.105919.
    Published • 10.1016/j.nbd.2022.105919
  7. Eva Alegre-Cortés; Alberto Giménez-Bejarano; Elisabet Uribe-Carretero; Marta Paredes-Barquero; André R. A. Marques; Mafalda Lopes-da-Silva; Otília V. Vieira; et al. "Delay of EGF-Stimulated EGFR Degradation in Myotonic Dystrophy Type 1 (DM1)". Cells 11 19 (2022): 3018-3018. http://dx.doi.org/10.3390/cells11193018.
    Published • 10.3390/cells11193018
  8. Barral, Duarte C.; Staiano, Leopoldo; Guimas Almeida, Cláudia; Cutler, Dan F.; Eden, Emily R.; Futter, Clare E.; Galione, Antony; et al. "Current methods to analyze lysosome morphology, positioning, motility and function". Traffic 23 5 (2022): 238-269. http://dx.doi.org/10.1111/tra.12839.
    Published • 10.1111/tra.12839
  9. Prieto Huarcaya, S.; Drobny, A.; Marques, André R. A.; Di Spiezio, Alessandro; Dobert, J. P.; Balta, D.; Werner, C.; et al. Corresponding author: Zunke, Friederike. "Recombinant pro-CTSD (cathepsin D) enhances SNCA/alpha-Synuclein degradation in alpha-Synucleinopathy models". Autophagy (2022):
    Published • 10.1080/15548627.2022.2045534
  10. Alves, Liliana S.; Marques, André R. A.; Padrão, Nuno; Carvalho, Filomena A.; Ramalho, José; Lopes, Catarina S.; Soares, Maria I. L.; et al. Corresponding author: Marques, André R. A.. "Cholesteryl hemiazelate causes lysosome dysfunction impacting vascular smooth muscle cell homeostasis". Journal of Cell Science 135 5 (2021): http://dx.doi.org/10.1242/jcs.254631.
    Published • 10.1242/jcs.254631
  11. Di Spiezio, Alessandro; Marques, André R. A.; Schmidt, Lina; Thießen, Niklas; Gallwitz, Lisa; Fogh, Jens; Bartsch, Udo; Saftig, Paul. Corresponding author: Saftig, Paul. "Analysis of cathepsin B and cathepsin L treatment to clear toxic lysosomal protein aggregates in neuronal ceroid lipofuscinosis". Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease 1867 10 (2021): 166205. http://dx.doi.org/10.1016/j.bbadis.2021.166205.
    Published • 10.1016/j.bbadis.2021.166205
  12. van der Lienden, Martijn J. C.; Aten, Jan; Marques, André R. A.; Waas, Ingeborg S. E.; Larsen, Per W. B.; Claessen, Nike; van der Wel, Nicole N.; et al. Corresponding author: Aerts, Johannes M. F. G.. "GCase and LIMP2 Abnormalities in the Liver of Niemann Pick Type C Mice". International Journal of Molecular Sciences 22 5 (2021): 2532. http://dx.doi.org/10.3390/ijms22052532.
    Open access • Published • 10.3390/ijms22052532
  13. Marques, André R. A.; Ramos, Cristiano; Machado-Oliveira, Gisela; Vieira, Otília V.. Corresponding author: Marques, André R. A.. "Lysosome (Dys)function in Atherosclerosis - A Big Weight on the Shoulders of a Small Organelle". Frontiers in Cell and Developmental Biology 9 (2021): http://dx.doi.org/10.3389/fcell.2021.658995.
    Published • 10.3389/fcell.2021.658995
  14. Boer, Daphne E.; Mirzaian, Mina; Ferraz, Maria J.; Zwiers, Kimberley C.; Baks, Merel V.; Hazeu, Marc D.; Ottenhoff, Roelof; et al. Corresponding author: Aerts, Johannes M.. "Human glucocerebrosidase mediates formation of xylosyl-cholesterol by ß-xylosidase and transxylosidase reactions". Journal of Lipid Research 62 (2021): 100018. http://dx.doi.org/10.1194/jlr.ra120001043.
    Published • 10.1194/jlr.ra120001043
  15. Machado-Oliveira, Gisela; Ramos, Cristiano; Marques, André R. A.; Vieira, Otília V.. Corresponding author: Vieira, Otília V.. "Cell Senescence, Multiple Organelle Dysfunction and Atherosclerosis". Cells 9 10 (2020): 2146. http://dx.doi.org/10.3390/cells9102146.
    Published • 10.3390/cells9102146
  16. Herrera Moro Chao, Daniela; Wang, Yanan; Foppen, Ewout; Ottenhoff, Roelof; van Roomen, Cindy; Parlevliet, Edwin T.; van Eijk, Marco; et al. Corresponding author: Yi, Chun-Xia. "The Iminosugar AMP-DNM Improves Satiety and Activates Brown Adipose Tissue Through GLP1". Diabetes 68 12 (2019): 2223-2234. http://dx.doi.org/10.2337/db19-0049.
    Published • 10.2337/db19-0049
  17. Marques, André R. A.; Di Spiezio, Alessandro; Thießen, Niklas; Schmidt, Lina; Grötzinger, Joachim; Lüllmann-Rauch, Renate; Damme, Markus; et al. Corresponding author: Saftig, Paul. "Enzyme replacement therapy with recombinant pro-CTSD (cathepsin D) corrects defective proteolysis and autophagy in neuronal ceroid lipofuscinosis". Autophagy 16 5 (2019): 811-825. http://dx.doi.org/10.1080/15548627.2019.1637200.
    Published • 10.1080/15548627.2019.1637200
  18. Marques, André R. A.; Saftig, Paul. Corresponding author: Saftig, Paul. "Lysosomal storage disorders - challenges, concepts and avenues for therapy: beyond rare diseases". Journal of Cell Science 132 2 (2019): jcs221739. http://dx.doi.org/10.1242/jcs.221739.
    Published • 10.1242/jcs.221739
  19. van Rooden, Eva J.; van Esbroeck, Annelot C. M.; Baggelaar, Marc P.; Deng, Hui; Florea, Bogdan I.; Marques, André R. A.; Ottenhoff, Roelof; et al. Corresponding author: van der Stelt, Mario. "Chemical Proteomic Analysis of Serine Hydrolase Activity in Niemann-Pick Type C Mouse Brain". Frontiers in Neuroscience 12 (2018): http://dx.doi.org/10.3389/fnins.2018.00440.
    Published • 10.3389/fnins.2018.00440
  20. Aerts, Johannes M. F. G.; Ferraz, Maria J; Mirzaian, Mina; Gaspar, Paulo; Oussoren, Saskia V; Wisse, Patrick; Chi-Lin Kuo; et al. "Lysosomal Storage Diseases. For Better or Worse: Adapting to Defective Lysosomal Glycosphingolipid Breakdown". eLS (2017): http://onlinelibrary.wiley.com/doi/10.1002/9780470015902.a0027592/abstract.
    Published • 10.1002/9780470015902.a0027592
  21. Mirzaian, M.; Wisse, P.; Ferraz, M.J.; Marques, André R. A.; Gaspar, P.; Oussoren, S.V.; Kytidou, K.; et al. Corresponding author: Aerts, J.M.. "Simultaneous quantitation of sphingoid bases by UPLC-ESI-MS/MS with identical 13C-encoded internal standards". Clinica Chimica Acta 466 (2017): 178-184. http://dx.doi.org/10.1016/j.cca.2017.01.014.
    Published • 10.1016/j.cca.2017.01.014
  22. Marques, André R. A.; Willems, Lianne I.; Herrera Moro, Daniela; Florea, Bogdan I.; Scheij, Saskia; Ottenhoff, Roelof; van Roomen, Cindy P. A. A.; et al. Corresponding author: Aerts, Johannes M. F. G.. "A Specific Activity-Based Probe to Monitor Family GH59 Galactosylceramidase, the Enzyme Deficient in Krabbe Disease". ChemBioChem 18 4 (2017): 402-412. http://dx.doi.org/10.1002/cbic.201600561.
    Published • 10.1002/cbic.201600561
  23. Mirzaian, Mina; Wisse, Patrick; Ferraz, Maria J.; Marques, André R. A.; Gabriel, Tanit L.; van Roomen, Cindy P.A.A.; Ottenhoff, Roelof; et al. Corresponding author: Aerts, Johannes M.. "Accurate quantification of sphingosine-1-phosphate in normal and Fabry disease plasma, cells and tissues by LC-MS/MS with 13 C-encoded natural S1P as internal standard". Clinica Chimica Acta 459 (2016): 36-44. http://dx.doi.org/10.1016/j.cca.2016.05.017.
    Published • 10.1016/j.cca.2016.05.017
  24. Ferraz, Maria J.; Marques, André R. A.; Appelman, Monique D.; Verhoek, Marri; Strijland, Anneke; Mirzaian, Mina; Scheij, Saskia; et al. Corresponding author: Aerts, Johannes M.. "Lysosomal glycosphingolipid catabolism by acid ceramidase: formation of glycosphingoid bases during deficiency of glycosidases". FEBS Letters 590 6 (2016): 716-725. http://dx.doi.org/10.1002/1873-3468.12104.
    Published • 10.1002/1873-3468.12104
  25. Marques, André R. A.; Mirzaian, Mina; Akiyama, Hisako; Wisse, Patrick; Ferraz, Maria J.; Gaspar, Paulo; Ghauharali-van der Vlugt, Karen; et al. Corresponding author: Aerts, Johannes M.. "Glucosylated cholesterol in mammalian cells and tissues: formation and degradation by multiple cellular ß-glucosidases". Journal of Lipid Research 57 3 (2016): 451-463. http://dx.doi.org/10.1194/jlr.m064923.
    Published • 10.1194/jlr.m064923
  26. Ferraz, Maria J.; Marques, André R. A.; Gaspar, Paulo; Mirzaian, Mina; van Roomen, Cindy; Ottenhoff, Roelof; Alfonso, Pilar; et al. Corresponding author: Aerts, Johannes M.. "Lyso-glycosphingolipid abnormalities in different murine models of lysosomal storage disorders". Molecular Genetics and Metabolism 117 2 (2016): 186-193. http://dx.doi.org/10.1016/j.ymgme.2015.12.006.
    Published • 10.1016/j.ymgme.2015.12.006
  27. Marques, André R. A.; Gabriel, Tanit L.; Aten, Jan; van Roomen, Cindy P. A. A.; Ottenhoff, Roelof; Claessen, Nike; Alfonso, Pilar; et al. Corresponding author: van Eijk, Marco. "Gpnmb Is a Potential Marker for the Visceral Pathology in Niemann-Pick Type C Disease". PLOS ONE 11 1 (2016): e0147208. http://dx.doi.org/10.1371/journal.pone.0147208.
    Published • 10.1371/journal.pone.0147208
  28. Herrera Moro Chao, Daniela; Kallemeijn, Wouter W.; Marques, André R. A.; Orre, Marie; Ottenhoff, Roelof; van Roomen, Cindy; Foppen, Ewout; et al. Corresponding author: Aerts, Johannes M. F. G.. "Visualization of Active Glucocerebrosidase in Rodent Brain with High Spatial Resolution following In Situ Labeling with Fluorescent Activity Based Probes". PLOS ONE 10 9 (2015): e0138107. http://dx.doi.org/10.1371/journal.pone.0138107.
    Published • 10.1371/journal.pone.0138107
  29. Marques, André R. A.; Aten, Jan; Ottenhoff, Roelof; van Roomen, Cindy P. A. A.; Herrera Moro, Daniela; Claessen, Nike; Vinueza Veloz, María Fernanda; et al. Corresponding author: Aerts, Johannes M. F. G.. "Reducing GBA2 Activity Ameliorates Neuropathology in Niemann-Pick Type C Mice". PLOS ONE 10 8 (2015): e0135889. http://dx.doi.org/10.1371/journal.pone.0135889.
    Published • 10.1371/journal.pone.0135889
  30. Dahl, Maria; Doyle, Alexander; Olsson, Karin; Månsson, Jan-Eric; Marques, André R. A.; Mirzaian, Mina; Aerts, Johannes M; et al. Corresponding author: Karlsson, Stefan. "Lentiviral Gene Therapy Using Cellular Promoters Cures Type 1 Gaucher Disease in Mice". Molecular Therapy 23 5 (2015): 835-844. http://dx.doi.org/10.1038/mt.2015.16.
    Published • 10.1038/mt.2015.16
  31. Ferraz, Maria J.; Kallemeijn, Wouter W.; Mirzaian, Mina; Herrera Moro, Daniela; Marques, André R. A.; Wisse, Patrick; Boot, Rolf G.; et al. Corresponding author: Aerts, J.M.. "Gaucher disease and Fabry disease: New markers and insights in pathophysiology for two distinct glycosphingolipidoses". Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids 1841 5 (2014): 811-825. http://dx.doi.org/10.1016/j.bbalip.2013.11.004.
    Published • 10.1016/j.bbalip.2013.11.004
  32. Sorrentino, Vincenzo; Nelson, Jessica K.; Maspero, Elena; Marques, André R. A.; Scheer, Lilith; Polo, Simona; Zelcer, Noam. Corresponding author: Zelcer, Noam. "The LXR-IDOL axis defines a clathrin-, caveolae-, and dynamin-independent endocytic route for LDLR internalization and lysosomal degradation". Journal of Lipid Research 54 8 (2013): 2174-2184. http://dx.doi.org/10.1194/jlr.m037713.
    Published • 10.1194/jlr.m037713
  33. Kallemeijn, Wouter W.; Li, Kah-Yee; Witte, Martin D.; Marques, André R. A.; Aten, Jan; Scheij, Saskia; Jiang, Jianbing; et al. Corresponding author: Overkleeft, Herman S.. "Novel Activity-Based Probes for Broad-Spectrum Profiling of Retaining ß-Exoglucosidases In Situ and In Vivo". Angewandte Chemie International Edition 51 50 (2012): 12529-12533. http://dx.doi.org/10.1002/anie.201207771.
    Published • 10.1002/anie.201207771

Intellectual property

Patent
  1. Marques, André R. A.; Fogh, Jens; Saftig, Paul; Zunke, Friederike. 2019. "Treatment of neuronal ceroid lipofuscinosis". United States.
    Granted/Issued