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In 2021, I concluded my BSc in Biotechnology at the University of Aveiro (2018/2021) with a final grade of 17/20 (A in ECTS considering the prior four years). In the last year of my BSc, I did an internship as a research trainee with Dr. Ana Tavares and Dr. Miguel Neto at CICECO - Aveiro Institute of Materials and at the Department of Engineering and Materials, to develop the project “Oxidative enzymes immobilization on functionalized diamond surfaces” (graded 18/20), exploring the very first steps of a boron-doped diamond-based enzymatic biosensor for phenolic compounds with potential applications in monitoring neurologic biomarkers. Next, I completed the MSc in Biochemistry and Biomedicine (2021/2024) at the Faculty of Sciences of the University of Lisbon with a final grade of 19/20. In my master’s project, I worked with Dr. Sandra Vaz at Instituto de Medicina Molecular, Faculty of Medicine of the University of Lisbon, in the project “Role of astrocytes in the endocannabinoid-mediated long-term depression in the medial prefrontal cortex (mPFC)“ (graded 20/20). Our aim was to unveil the astroglial CB1R role in the synaptic plasticity of mPFC and its translational value for depressive behavior. Briefly, we proposed a new mechanism behind long-term depression (LTD) maintenance where astrocytes respond to endocannabinoids by increasing their intracellular Ca2+ frequency and decreasing glutamate uptake, ultimately shaping LTD. This work resulted in two publications as contributing author: one already submitted for peer-reviewed publication in Nature Communications journal, in collaboration with the highly regarded Dr. Alfonso Araque (Nat. Commun, doi:10.3390/cells12030381); and another still under preparation reviewing the astrocyte’s contribution to neuroplastic alterations underlying depression highlighting the glutamatergic/endocannabinoid systems. During the last year, I presented this work in several science communications, including three international conferences (FENS Regional Meeting 2023; VII symposium of Portuguese Glial Network and AIMS Meeting 2023) and a national oral communication (53º Encontro da Sociedade Portuguesa de Farmacologia). Additionally, I had the opportunity to participate in several workshops, including the highly competitive “Synaptic function in epileptic disorders” workshop (EpiEpiNet) hosted by Sapienza University in Rome. I also did a Laboratory Animal Science Course for Function A (+D) (former FELASA Category B) CONGENTO to become fully independent on animal manipulation. Since 2023 I have been a member of Sociedade Portuguesa de Neurociências, Sociedade Portuguesa de Farmacologia, and Portuguese Glial Network. Currently, I am visiting Dr. Luisa Pinto’s lab, whose work focuses on novel therapeutics for depression with a link to neuro-glial plasticity. Here, I learned new concepts and techniques while having the opportunity to engage in the team's ongoing projects. Moreover, I was recentrly awarded with a PhD Studentship (2024.01292.BDANA) by FCT, to develop the project "AstroMap: Understanding Adult Astrogliogenesis in Health and Depression" under the supervision of Dr. Luisa Pinto, Dr. Sandra Vaz and Dr. Teresa Canedo. During this period, I was also able to participate in the organizing committee of the VIII symposium of the Portuguese Glial Network and to enroll in a science communication program (Scientist Returns to School) that promotes the communication of science to children through interactive workshops. To date, I already conducted two workshops in Escola Básica de Arões Santa-Cristina and in Escola Básica de Arões São-Romão where we playfully explored how the brain works.
Identification

Personal identification

Full name
Leandro José Rodrigues Freitas
Date of birth
2000/05/14

Citation names

  • Rodrigues-Freitas, Leandro

Author identifiers

Ciência ID
8113-FAB2-51B9
ORCID iD
0000-0002-4374-1816

Email addresses

  • id11570@alunos.uminho.pt (Professional)

Telephones

Telephone
  • 217999411 Ext.: 47126 (Professional)

Addresses

  • Instituto de Farmacologia e Neurociências, Faculdade de Medicina da Universidade de Lisboa, Avenida Professor Egas Moniz, 1649-028, Lisboa, Lisboa, Portugal (Professional)
  • Life and Health Sciences Research Institute (ICVS) School of Medicine, University of Minho, Campus de Gualtar, 4710-057, Gualtar, Braga, Portugal (Professional)

Websites

Knowledge fields

  • Medical and Health Sciences - Basic Medicine - Neurosciences

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese (Mother tongue)
English Upper intermediate (B2) Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Education
Degree Classification
2025/02/03 - 2030/02/02
Ongoing
Biomedicina e Ciências da Saúde (Doutoramento)
Universidade do Minho, Escola de Medicina, Portugal
2025/05/26 - 2025/06/09
Concluded
Integrative Strategies And Practical Skills In Neural Regenerative Medicine (Curso de doutoramento (conclusão de unidades curriculares))
Universidade do Minho, Escola de Medicina, Portugal
2025/05/12 - 2025/05/23
Concluded
Surgery, Neurosurgery and Advanced Anesthesia in Laboratory Rodents (Curso de doutoramento (conclusão de unidades curriculares))
Universidade do Minho, Escola de Medicina, Portugal
2024/07/29 - 2025/05/05
Concluded
Curso de Competências em Investigação Científica (Outros)
Universidade do Minho, Escola de Medicina, Portugal
2021 - 2023
Concluded
Bioquimica e Biomedicina (Mestrado integrado)
Universidade de Lisboa, Portugal
"Role of astrocytes in the endocannabinoid-mediated long-term depression in the medial prefrontal cortex" (THESIS/DISSERTATION)
19
2022/11/09 - 2022/11/21
Concluded
Course on Laboratory Animal Sciences (EU Functions A+D) (Outros)
Congento Consortium for Genetically Tractable Organisms, Portugal

Universidade de Lisboa Instituto de Medicina Molecular, Portugal
2018 - 2021
Concluded
Biotecnologia (Licenciatura)
Universidade de Aveiro, Portugal
"Immobilization of oxidative enzymes in functionalized diamond" (THESIS/DISSERTATION)
17
Affiliation

Science

Category
Host institution
Employer
2024/10/14 - 2025/04/13 Contracted Researcher (Research) Universidade do Minho Instituto de Investigação em Ciências da Vida e Saúde, Portugal
Universidade do Minho Instituto de Investigação em Ciências da Vida e Saúde, Portugal
2023/11/26 - 2024/09/17 Visiting Researcher (Research) Universidade do Minho Instituto de Investigação em Ciências da Vida e Saúde, Portugal
Universidade do Minho, Escola de Medicina, Portugal
2022/09/11 - 2023/09/11 Researcher (Research) Universidade de Lisboa Instituto de Medicina Molecular, Portugal
Universidade de Lisboa Faculdade de Medicina, Portugal
2020/10/06 - 2021/07/02 Research Trainee (Research) Universidade de Aveiro CICECO, Portugal
Universidade de Aveiro, Portugal

Positions / Appointments

Category
Host institution
Employer
2025/02/26 - Current Network of European Neuroscience Schools (NENS) Student Ambassador Network of European Neuroscience Schools (NENS), Belgium
2025/02/17 - Current Scientist ambassador in Município de Fafe Native Scientists, Portugal
Projects

Grant

Designation Funders
2025 - 2029 AstroMap: Understanding Adult Astrogliogenesis in Health and Depression
2024.01292.BDANA
PhD Student Fellow
Universidade do Minho Instituto de Investigação em Ciências da Vida e Saúde, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2024/10/14 - 2025/04/13 Financiamento FCT das Unidades de I&D|UIDP/50026/2020
UIDP/50026/2020_2023_ICVS
UMINHO/BIM-CNCG/2024/164
Research Fellow
Universidade do Minho Instituto de Investigação em Ciências da Vida e Saúde, Portugal
Fundação para a Ciência e a Tecnologia
Outputs

Publications

Conference poster
  1. Rodrigues-Freitas, L.; Canedo, T.; Vaz, Sandra H.; Pinto, Luisa. "AstroMap: First Glimpses of Adult Astrogliogenesis in Health and Depression". 2025.
  2. Figueiredo, Adriana; Monteiro-Pacheco, Ana; Rodrigues-Freitas, L.; Pinto, Luisa; Canedo, T.. "Desvendando a Especificidade Viral para a Marcação Seletiva de Novos Astrócitos Gerados no Hipocampo Adulto: Uma Abordagem Inovadora". Paper presented in MEEting 2025, 2025.
  3. Gonçalves-Ribeiro, Joana; Rodrigues-Freitas, L.; Nanclares C; Costa-Pinto, Sara; Sebastião, AM; Araque, Alfonso; Vaz SH. Corresponding author: Gonçalves-Ribeiro, Joana. "CB1R MODULATES GLUTAMATE TRANSPORTERS: SHAPING LTD". Paper presented in VIII Symposium Portuguese Glial Network, 2024.
  4. Bernardo L. Tavares; Rodrigues-Freitas, L.; R. Fernandes, Diogo; Ebinuma, Valeria; João C. F. Nunes; Neves, Márcia C.; Girão, Ana V.; Neto, Miguel Ângelo da Costa; Tavares, Ana P. M.. Corresponding author: Bernardo L. Tavares. "Boron-doped diamond surface as a platform for the development of an enzymatic biosensor". Paper presented in XXVIII Encontro Nacional da Sociedade Portuguesa de Química, 2023.
  5. Rodrigues-Freitas, L.; Gonçalves-Ribeiro, Joana; Sebastião, AM; Vaz, Sandra H.. Corresponding author: Rodrigues-Freitas, L.. "Astrocytic CB1R impact on Long-Term Depression in the medial Prefrontal Cortex". Paper presented in FENS REGIONAL MEETING 2023, 2023.
  6. Gonçalves-Ribeiro, Joana; Rodrigues-Freitas, L.; Savchak O; Costa-Pinto, Sara; Sebastian R; Lillo A; Navarro-Brugal G; et al. Corresponding author: Gonçalves-Ribeiro, Joana. "Astroglial CB1R activity is dictated by adenosine receptors in the rodent mPFC". Paper presented in FENS REGIONAL MEETING 2023, 2023.
  7. Rodrigues-Freitas, L.; Gonçalves-Ribeiro, Joana; Sebastião, AM; Vaz, Sandra H.. Corresponding author: Rodrigues-Freitas, L.. "The relevance of astroglial CB1R in the rodent mPFC synaptic plasticity". Paper presented in VII Symposium of the Portuguese Glial Network, 2023.
  8. Gonçalves-Ribeiro, Joana; Nanclares C; Costa-Pinto, Sara; Neuparth-Sottomayor, Mariana; Rodrigues-Freitas, L.; Gualdino M; Sebastião, AM; Araque, Alfonso; Vaz SH. Corresponding author: Gonçalves-Ribeiro, Joana. "CB1R downregulates glutamate transporters activity in a astroglial Ca2+-manner in the rodent mPFC". Paper presented in Glial Portuguese Meeting VII Symposium, 2023.
  9. Rodrigues-Freitas, L.; Gonçalves-Ribeiro, Joana; Sebastião, AM; Vaz, Sandra H.. "Unveiling the role of astrocytes in the eCB Long-Term Depression in the rodent mPFC". Paper presented in AIMS Meeting 2023 Research Competition, 2023.
  10. Tavares, Bernardo L.; Rodrigues-Freitas, L.; R. Fernandes, Diogo; Ebinuma, Valeria; João C. F. Nunes; Neves, Márcia C.; Girão, Ana V.; Neto, Miguel Ângelo da Costa; Tavares, Ana P. M.. Corresponding author: Tavares, Bernardo L.. "Development of an Enzymatic Biosensor Based on Boron-doped Diamond Surface". Paper presented in XXVI Encontro Galego-Portugués de Química, 2022.
  11. Tavares, Bernardo L.; Rodrigues-Freitas, L.; R. Fernandes, Diogo; Ebinuma, Valeria; João C. F. Nunes; Neves, Márcia C.; Girão, Ana V.; Neto, Miguel Ângelo da Costa; Tavares, Ana P. M.. Corresponding author: Tavares, Bernardo L.. "Development of an Enzymatic Biosensor Based on Boron-doped Diamond Surface". Paper presented in CICECO Jornadas 22 – Hand in Hand with Society, 2022.
Journal article
  1. Bernardo L. Tavares; Diogo R. Fernandes; Leandro Rodrigues-Freitas; João C.F. Nunes; Ana V. Girão; Márcia C. Neves; António J.S. Fernandes; et al. "Simple adsorption-based immobilization of l-asparaginase on boron-doped diamond film". Surfaces and Interfaces (2025): https://doi.org/10.1016/j.surfin.2025.107606.
    10.1016/j.surfin.2025.107606
  2. Gonçalves-Ribeiro, Joana; Rodrigues-Freitas, Leandro; Nanclares, Camen; Neuparth-Sottomayor, Mariana; Costa-Pinto, Sara; Gualdino, Madalena A.; Magalhães, Daniela M.; et al. "Modulation of Astrocytic EAATS by CB1 Receptor Shapes Endocannabinoid Mediated Long Term Depression enabling Antidepressive-like Behaviour". Molecular Psychiatry (2025): https://doi.org/10.21203/rs.3.rs-4281560/v1.
    Under revision
Thesis / Dissertation
  1. "Role of astrocytes in the endocannabinoid-mediated long-term depression in the medial prefrontal cortex". Master, Universidade de Lisboa Instituto de Medicina Molecular, 2024.
  2. "Oxidative enzymes immobilization on functionalized diamond surfaces". Degree, Universidade de Aveiro CICECO, 2021.
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2023/02/08 Role of astrocytes in the endocannabinoid-mediated long-term depression in the medial prefrontal cortex 5º Encontro Anual do Mestrado em Bioquímica e Biomedicina
Faculdade de Ciências da Universidade de Lisboa (Lisboa, Portugal)
2023/02/01 Role of astrocytes in the endocannabinoid-mediated long-term depression in the medial prefrontal cortex 53º Encontro da Sociedade Portuguesa de Farmacologia
Sociedade Portuguesa de Farmacologia (Coimbra, Portugal)
2022/01/16 Role of astrocytes in the endocannabinoid-mediated long-term depression in the mPFC Neurosciences Seminar
Instituto de Farmacologia e Neurociência, iMM, FMUL (Lisboa, Portugal)

Event organisation

Event name
Type of event (Role)
Institution / Organization
2024/04/05 - Current 8th Symposium of the Portuguese Glial Network (2024/04/05)
Symposium (Member of the Organising Committee)
Universidade do Minho Instituto de Investigação em Ciências da Vida e Saúde, Portugal
2023/03/11 - 2023/03/11 Activity in primary schools of Município de Fafe: Escola Básica de Arões Santa Cristina (Turma M34) and Escola Básica de Arões São Romão (Turma ASR4) (2023/03/11 - 2023/03/11)
Workshop (Co-organisor)
Native Scientists, Portugal

Event participation

Activity description
Type of event
Event name
Institution / Organization
2025/03/24 - 2025/03/24 Course in Bioethics and Scientific Integrity
Seminar
Bioethics and Scientific Integrity
Universidade do Minho Escola de Ciências da Saúde, Portugal
2025/03/13 - 2025/03/13 Activity "Quiz" with 2nd year students of the Colégio Alfacoop
Other
Brain Awareness Week
Universidade do Minho Instituto de Investigação em Ciências da Vida e Saúde, Portugal
2024/07/04 - 2024/07/05 ICVS Annual Retreat 2024
Meeting
ICVS Retreat 2024
Universidade do Minho Instituto de Investigação em Ciências da Vida e Saúde, Portugal
2023/08/05 - 2023/08/06 The Astrocytic Control of Brain Circuits and Behavior workshop
Workshop
ISN-ESN Meeting Satellite Workshop “Astrocytic Control of Brain Circuits and Behavior”
Universidade do Minho Instituto de Investigação em Ciências da Vida e Saúde, Portugal
2023/05/03 - 2023/05/05 FENS Regional Meeting 2023
Conference
FENS Regional Meeting 2023
Federation of European Neuroscience Societies, Belgium

Algarve Biomedical Center Research Institute, Portugal
2023/05/02 - 2023/05/02 7th Symposium of the Portuguese Glial Network
Symposium
VII Symposium of the Portuguese Glial Network
Federation of European Neuroscience Societies, Belgium
2023/04/16 - 2023/04/16 AIMS Meeting [Annual International (bio)Medical Students Meeting] workshop
Workshop
Workshop on Paediatric Neurosurgery (Theoretical/Practical)
Universidade de Lisboa Faculdade de Medicina, Portugal
2023/04/13 - 2023/04/16 AIMS Meeting [Annual International (bio)Medical Students Meeting]
Congress
AIMS Meeting 2023 [Annual International (bio)Medical Students Meeting]
Universidade de Lisboa Faculdade de Medicina, Portugal
2023/04/15 - 2023/04/15 AIMS Meeting [Annual International (bio)Medical Students Meeting] workshop
Workshop
Workshop on Palliative Care (Theoretical/Practical)
Universidade de Lisboa Faculdade de Medicina, Portugal
2023/03/07 - 2023/03/07 Portuguese Glial Network workshop
Workshop
Workshop on electrophysiology recordings, Portuguese Glial Network
Universidade de Lisboa Instituto de Medicina Molecular, Portugal
2022/11/25 - 2022/11/25 Rede SAÚDE workshop
Workshop
Terapia Génica – O Presente e o Futuro
Universidade de Lisboa, Portugal
2022/10/18 - 2022/10/18 6th Symposium of the Portuguese Glial Network
Symposium
VI Symposium of the Portuguese Glial Network
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal

Federation of European Neuroscience Societies, Belgium
2022/09/23 - 2022/09/24 EpiEpiNet Conference
Congress
From genes, synapses, and circuits to pave the way for novel drugs and strategies
Universidade de Lisboa Instituto de Medicina Molecular, Portugal
2022/09/19 - 2022/09/20 EpiEpiNet synaptic funcion workshop
Workshop
Synaptic function in epileptic disorders: from basic to clinical implications
Università degli Studi di Roma La Sapienza Dipartimento di Fisiologia e Farmacologia Vittorio Erspamer, Italy

Association member

Society Organization name Role
2023 - Current Sociedade Portuguesa de Neurociências Membro
2023 - Current Sociedade Portuguesa de Farmacologia Membro
2023 - Current Rede Glial Portuguesa Membro

Mentoring / Tutoring

Topic Student name
2025/03/23 - 2025/06/23 Internship Report (Bachelor in Biochemistry, University of Minho). "Unveiling the impact of Adult-born Astrocytes Activation in Behavioural Outcomes." Grade: Luísa Gonçalves Insua Pereira
2025/03/17 - 2025/06/17 Project 3 (Integrated Master in Medicine, University of Minho). "Mapping Adult Astrogliogenesis Influence in Health and Depression." Grade: Adriana Filipa Martins Vieira
2025/03/10 - 2025/04/17 Student-Selected Modules 1 (Integrated Master in Medicine, University of Algarve). "Desvendando a Especificidade Viral para a Marcação Seletiva de Novos Astrócitos Gerados no Hipocampo Adulto: Uma Abordagem Inovadora" Adriana Marisa da Silva Figueiredo
2024/12/04 - 2025/03/14 Project 2 (Integrated Master in Medicine, University of Minho). "Decoding the role of mouse adult-born astrocytes in hippocampal plasticity: from health to depression." Grade: 19 out of 20 Inês Sofia Oliveira Maia
2019/09/19 - 2021/07/26 Student mentor Hugo Santos, Mariana Pinto, Rafael Mascarenhas, Alexandre Santiago
Distinctions

Award

2024 Merit and Excellence Scholarship 2024, Município de Fafe
Município de Fafe, Portugal