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Noélia Custódio completed a BSc in Biochemistry at the Faculty of Sciences, University of Lisbon and a PhD in Biomedical Sciences at the Faculty do Medicine, University of Lisbon. Currently she is a Researcher at Instituto de Medicina Molecular João Lobo Antunes (iMM-JLA Lisboa) and Assistant Professor at the Faculty of Medicine, University of Lisbon. Works in Biomedical Sciences with emphasis in Human Molecular Genetics and Oncobiology to uncover molecular pathways and mechanisms implicating RNA in human health and disease.
Identification

Personal identification

Full name
Noélia Maria Fernandes Custódio

Citation names

  • Custódio, Noélia

Author identifiers

Ciência ID
8615-44B3-7645
ORCID iD
0000-0001-9230-9870
Scopus Author Id
6508187568

Email addresses

  • noelia.custodio@medicina.ulisboa.pt (Professional)

Telephones

Telephone
  • 217999503 (Professional)

Addresses

  • Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa. Av. Prof Egas Moniz, 1649-028, Lisboa, Lisboa, Portugal (Professional)

Websites

Knowledge fields

  • Natural sciences - Biological Sciences - Cell Biology
  • Natural sciences - Biological Sciences - Molecular Biology
  • Medical and Health Sciences - Basic Medicine - Human Genetics

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese (Mother tongue)
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Education
Degree Classification
2008/06/19
Concluded
 Ciências Biomédicas (Doutoramento)
Major in Especialidade: Ciências Morfológicas
Universidade de Lisboa Faculdade de Medicina, Portugal
"Quality Control of Gene Expression in the Mammalian Cell Nucleus" (THESIS/DISSERTATION)
 Approved with honors
2003/10/17
Concluded
 Provas de Aptidão Pedagógica e Capacidade Científica (Outros)
Universidade de Lisboa Faculdade de Medicina, Portugal
 Approved with honors
1989/09/30 - 1994/09/30
Concluded
 Biochemistry (Licenciatura)
Universidade de Lisboa Faculdade de Ciências, Portugal
"Arquitectura Funcional do Núcleo de Células de Mamífero: visualização de cromossomas, genes e produtos de transcrição" (THESIS/DISSERTATION)
 16
Affiliation

Science

Category
Host institution 		Employer
2004 - Current Researcher (Research) Instituto de Medicina Molecular João Lobo Antunes, Portugal
Instituto de Medicina Molecular João Lobo Antunes, Portugal

Teaching in Higher Education

Category
Host institution 		Employer
2008/06/19 - Current Assistant Professor (University Teacher) Universidade de Lisboa Faculdade de Medicina, Portugal
Universidade de Lisboa Faculdade de Medicina, Portugal
2003/10/18 - 2008/06/19 Assistant (University Teacher) Universidade de Lisboa Faculdade de Medicina, Portugal
1999/07/18 - 2003/10/17 Trainee Assistant (University Teacher) Universidade de Lisboa Faculdade de Medicina, Portugal
Universidade de Lisboa Faculdade de Medicina, Portugal

Others

Category
Host institution 		Employer
1997 - 1999 Bolseira de Doutoramento (PRAXIS XXI/BD/9439/96) Junta Nacional de Investigação Científica e Tecnológica (JNICT), Portugal, Portugal
Universidade de Lisboa Faculdade de Medicina, Portugal
1995 - 1996 Bolseira de Investigação (PRAXIS/2/2.1/SAU/06/94/BIC/908) Junta Nacional de Investigação Científica e Tecnológica (JNICT), Portugal, Portugal
Instituto de Histologia e Embriologia, Faculdade de Medicina, Universidade de Lisboa, Portugal
Projects

Grant

Designation Funders
2018/07/01 - 2021/06/30 Gene-edited organoids to study the functional impact of BRCA1 and BRCA2 mutations
PTDC/MED-ONC/29469/2017
Principal investigator
Instituto de Medicina Molecular João Lobo Antunes, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2016/05/01 - 2019/10/31 Making sense of antisense transcription
PTDC/BEX-BCM/5899/2014
Researcher
Instituto de Medicina Molecular João Lobo Antunes, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2014/03/01 - 2017/06/30 Epigenetics and alternative splicing: role in immune T cell differentiation and leukemogenesis
FCT-ANR/BIM-ONC/0009/2013
Researcher
Instituto de Medicina Molecular João Lobo Antunes, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2012 - 2015 Splicing and stochasticity in programmed cell death decisions.
FCT-PTDC/SAU-GMG/118180/2010)
Researcher
Instituto de Medicina Molecular João Lobo Antunes, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2011/01/01 - 2013/12/31 The role of RNA polymerase II in splicing regulation
PTDC/SAU-GMG/113440/2009
Researcher
Instituto de Medicina Molecular João Lobo Antunes, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2010/01/01 - 2011/12/31 Transcription-coupled mechanisms of gene regulation
PTDC/BIA-BCM/101575/2008
Researcher
Instituto de Medicina Molecular João Lobo Antunes, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2007/09/01 - 2010/08/31 Mechanisms of splicing regulation by U2AF proteins
PTDC/SAU-GMG/69739/2006
Researcher
Instituto de Medicina Molecular João Lobo Antunes, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2005/04/01 - 2008/06/30 Cell Bio of messenger RNA biogenesis
POCI/V.5/B0019/2005
Researcher
Instituto de Medicina Molecular João Lobo Antunes, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2005 - 2007 Mechanisms of regulation of mRNA processing and transport in the nucleus
FCT-POCI/SAU-MMO/57700/2004
Researcher
Instituto de Medicina Molecular João Lobo Antunes, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2001 - 2004 RNOMICS-An integrated analysis of links between pathways of RNA metabolism in the post-genomic era
QLRT-2000-01554
Researcher
Universidade de Lisboa Faculdade de Medicina, Portugal
Concluded
2001 - 2004 Genetic Diseases and RNA Processing
SAPIENS 36547/99
Researcher
Universidade de Lisboa Faculdade de Medicina, Portugal
 Junta Nacional de Investigação Científica e Tecnológica (JNICT), Portugal
Concluded
2000 - 2002 Gene Expression in 4 dimensions
PRAXIS BIO/12085/98
Researcher
Universidade de Lisboa Faculdade de Medicina, Portugal
 Junta Nacional de Investigação Científica e Tecnológica (JNICT), Portugal
Concluded
1995 - 1996 Nuclear Organization during interphase and mitosis
PRAXIS SAU/06/94
Scientific Initiation Fellow
Universidade de Lisboa Faculdade de Medicina, Portugal
 Junta Nacional de Investigação Científica e Tecnológica (JNICT), Portugal
Concluded
Outputs

Publications

Book
  1. Custódio, N.; Carvalho, C.; Carneiro, T.; Carmo-Fonseca, M.. In Situ Hybridization for Simultaneous Detection of DNA, RNA, and Protein. 2006.
    10.1016/B978-012164730-8/50172-6
Journal article
  1. Tammer L; Hameiri O; Keydar I; Roy VR; Ashkenazy-Titelman A; Custódio N; Sason I; et al. "Gene architecture directs splicing outcome in separate nuclear spatial regions.". Mol Cell. 82 5 (2022): 1021-1034.e8. https://doi.org/10.1016/j.molcel.2022.02.001.
    Published
  2. Noélia Custódio; Rosina Savisaar; Celia Carvalho; PEDRO BAK-GORDON; Maria I. Ribeiro; Joana Tavares; Paula B. Nunes; et al. "Expression Profiling in Ovarian Cancer Reveals Coordinated Regulation of BRCA1/2 and Homologous Recombination Genes". Biomedicines 10 2 (2022): https://www.mdpi.com/2227-9059/10/2/199.
    Open access • 10.3390/biomedicines10020199
  3. Vaz-Drago, R.; Custódio, N.; Carmo-Fonseca, M.. "Deep intronic mutations and human disease". Human Genetics (2017): 1-19. http://www.scopus.com/inward/record.url?eid=2-s2.0-85019143016&partnerID=MN8TOARS.
    10.1007/s00439-017-1809-4
  4. Custódio, N.; Carmo-Fonseca, M.. "Co-transcriptional splicing and the CTD code". Critical Reviews in Biochemistry and Molecular Biology 51 5 (2016): 395-411. http://www.scopus.com/inward/record.url?eid=2-s2.0-84992360016&partnerID=MN8TOARS.
    10.1080/10409238.2016.1230086
  5. Vaz-Drago, R.; Pinheiro, M.T.; Martins, S.; Enguita, F.J.; Carmo-Fonseca, M.; Custódio, N.. "Transcription-coupled RNA surveillance in human genetic diseases caused by splice site mutations". Human Molecular Genetics 24 10 (2015): 2784-2795. http://www.scopus.com/inward/record.url?eid=2-s2.0-84925874353&partnerID=MN8TOARS.
    10.1093/hmg/ddv039
  6. De Almeida, S.F.; García-Sacristán, A.; Custódio, N.; Carmo-Fonseca, M.. "A link between nuclear RNA surveillance, the human exosome and RNA polymerase II transcriptional termination". Nucleic Acids Research 38 22 (2010): 8015-8026. http://www.scopus.com/inward/record.url?eid=2-s2.0-78650445002&partnerID=MN8TOARS.
    10.1093/nar/gkq703
  7. Custódio, N.; Vivo, M.; Antoniou, M.; Carmo-Fonseca, M.. "Splicing- and cleavage-independent requirement of RNA polymerase II CTD for mRNA release from the transcription site". Journal of Cell Biology 179 2 (2007): 199-207. http://www.scopus.com/inward/record.url?eid=2-s2.0-35548953842&partnerID=MN8TOARS.
    10.1083/jcb.200612109
  8. Custódio, N.; Antoniou, M.; Carmo-Fonseca, M.. "Abundance of the largest subunit of RNA polymerase II in the nucleus is regulated by nucleo-cytoplasmic shuttling". Experimental Cell Research 312 13 (2006): 2557-2567. http://www.scopus.com/inward/record.url?eid=2-s2.0-33745835437&partnerID=MN8TOARS.
    10.1016/j.yexcr.2006.04.018
  9. Custódio, N.; Carvalho, C.; Condado, I.; Antoniou, M.; Blencowe, B.J.; Carmo-Fonseca, M.. "In vivo recruitment of exon junction complex proteins to transcription sites in mammalian cell nuclei". RNA 10 4 (2004): 622-633. http://www.scopus.com/inward/record.url?eid=2-s2.0-17644443879&partnerID=MN8TOARS.
    10.1261/rna.5258504
  10. Mühlemann, O.; Mock-Casagrande, C.S.; Wang, J.; Li, S.; Custódio, N.; Carmo-Fonseca, M.; Wilkinson, M.F.; Moore, M.J.. "Precursor RNAs harboring nonsense codons accumulate near the site of transcription". Molecular Cell 8 1 (2001): 33-43. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034886974&partnerID=MN8TOARS.
    10.1016/S1097-2765(01)00288-X
  11. Custödio, N.; Carmo-Fonseca, M.. "Quality control of gene expression in the nucleus". Journal of Cellular and Molecular Medicine 5 3 (2001): 267-275. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035527330&partnerID=MN8TOARS.
  12. Custódio, N.; Carmo-Fonseca, M.; Geraghty, F.; Pereira, H.S.; Grosveld, F.; Antoniou, M.. "Inefficient processing impairs release of RNA from the site of transcription". EMBO Journal 18 10 (1999): 2855-2866. http://www.scopus.com/inward/record.url?eid=2-s2.0-0041013167&partnerID=MN8TOARS.
    10.1093/emboj/18.10.2855
  13. Carmo-Fonseca, M.; Custódio, N.; Calado, A.. "Intranuclear trafficking of messenger RNA". Critical Reviews in Eukaryotic Gene Expression 9 3-4 (1999): 213-219. http://www.scopus.com/inward/record.url?eid=2-s2.0-0342948918&partnerID=MN8TOARS.
  14. Carmo-Fonseca, M.; Cunha, C.; Custódio, N.; Carvalho, C.; Jordan, P.; Ferreira, J.; Parreira, L.. "The topography of chromosomes and genes in the nucleus". Experimental Cell Research 229 2 (1996): 247-252. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030589487&partnerID=MN8TOARS.
    10.1006/excr.1996.0367
  15. Elefanty, A.G.; Antoniou, M.; Custodio, N.; Carmo-Fonseca, M.; Grosveld, F.G.. "GATA transcription factors associate with a novel class of nuclear bodies in erythroblasts and megakaryocytes". EMBO Journal 15 2 (1996): 319-333. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030068286&partnerID=MN8TOARS.
Thesis / Dissertation
  1. Custódio, Noélia Maria Fernandes, 1971-. "Quality control of gene expression in the mammalian cell nucleus". PhD, Universidade de Lisboa Faculdade de Medicina, 2008. http://sibul.reitoria.ul.pt/F/?func=item-global&doc_library=ULB01&type=03&doc_number=000532476.
Activities

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2020/09 - 2021/09 Patient-derived pancreatic cancer organoids for modeling drug response
Supervisor of Ana Catarina Cardoso Lopes
 Tecnologias Moleculares em Saúde (Master)
Instituto Politécnico de Lisboa Escola Superior de Tecnologia da Saúde de Lisboa, Portugal
2020/09 - 2021/06/07 Expression profiles of BRCA1/2 and homologous recombination genes in ovarian cancer
Co-supervisor of Maria Inês Fernandes Ribeiro
 Trabalho Final do Curso de Mestrado Integrado em Medicina (Master)
Universidade de Lisboa Faculdade de Medicina, Portugal
2019/09 - 2021/03 Ovarian cancer organoids for modeling drug response and discovering novel BRCA biomarkers
Supervisor of Beatriz Galvão
 Biologia Molecular e Genética (Master)
Universidade de Lisboa Faculdade de Ciências, Portugal
2018 - 2019 How the lncRNA Zeb2-NAT regulates the expression of Zeb2
Supervisor of Miguel Francisco Cruz Romano Guedes
 Ciências Biofarmacêuticas (Master)
Universidade de Lisboa Faculdade de Farmácia, Portugal
2013 - 2018 Co-transcriptional quality control of mRNA biogenesis : impact for human genetic diseases
Co-supervisor of Rita Catarina Vaz Drago
 Biomedical Sciences (PhD)
Universidade de Lisboa Faculdade de Medicina, Portugal
2014 - 2016 Epigenetics and alternative splicing
Co-supervisor of Soraia Vanessa Guerreiro da Silva
 Oncobiologia - Mecanismos Moleculares do Cancro (Master)
Universidade do Algarve, Portugal
2011 - 2012 The Nuclear Quality Control of Aberrant mRNA Transcripts in Human Genetic Disease
Co-supervisor of Marco Pinheiro
Industrial Placement of Genetics B.Sc. (Scientific initiation)
The University of Manchester School of Health Sciences, United Kingdom

Jury of academic degree

Topic
Role 		Candidate name (Type of degree)
Institution / Organization
2021/12/21 The role of cyclin-dependent kinases (CDKs) in co-transcriptional splicing through phosphorylation of the RNA Pol II carboxyl terminal domain (CTD).
(Thesis) Arguer
 Thais Souza Cunha (Master)
Universidade de Lisboa Faculdade de Farmácia, Portugal
2019/11/13 Single-molecule imaging of transcription dynamics
(Thesis) Main arguer
 Alda Filipa Queirós Oliveira e Silva (Master)
Universidade de Lisboa Faculdade de Ciências, Portugal
2018/11/14 Regulation of the alternative splicing of RAC1b in tumour cells
(Thesis) Main arguer
 Cláudia Alexandra da Silva Rodrigues (Master)
Universidade de Lisboa Faculdade de Ciências, Portugal

Course / Discipline taught

Academic session Degree Subject (Type) Institution / Organization
2009 - Current Oncobiology (Tronco Comum V. a) Oncobiologia) Medicina (Mestrado integrado) Universidade de Lisboa Faculdade de Medicina, Portugal
2007 - Current Molecular Biology of the Cell (Módulo I.I - Biologia Molecular, Celular e do Desenvolvimento Humano e Genética) Medicina (Mestrado integrado) Universidade de Lisboa Faculdade de Medicina, Portugal
1999 - 2006 Molecular Biology of the Cell Medicina (Licenciatura) Universidade de Lisboa Faculdade de Medicina, Portugal

Mentoring / Tutoring

Topic Student name
2020/01 - 2021/12 GAPIC research project for medical students " Why does BRCA loss of function predispose to tumor mainly in estrogen-related tissues?" Maria Grilo and Raquel Antunes
2020/01 - 2020/12 GAPIC research project for medical students "Looking beyond DNA II: Screening for mRNA biomarkers of response to PARP inhibition." Pedro Bak-Gordon
2018 - 2019 GAPIC research project for medical students "Looking beyond DNA: Characterizing the impact of the Portuguese BRCA2 founder mutation on mRNA processing using mammary organoids." Pedro Bak Gordon
2017 - 2018 GAPIC research project for medical students "An RNA-based screening for BRCA deficiency. " Maria Inês Fernandes Ribeiro
2012 - 2013 GAPIC research project for medical students "Avoiding the production of disease-causing proteins: is there a general mechanism for tethering mutated transcripts to the gene template? " Catarina Alves do Vale
2011 - 2012 GAPIC research project for medical students "Co-transcriptional effects of premature stop codons associated with genetic diseases." Vanessa Sofia de Oliveira Artilheiro
2006 - 2007 GAPIC research project for medical students "Splicing and Aging: effect of the cellular levels of splicing factors." Duarte Martins