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I am a talented early career researcher who specialises in combining interdisciplinary knowledge to tackle human diseases such as cancer. My specific research ambitions are to harness the immune system to develop new or improved immunotherapeutic strategies. My academic path has spanned several disciplines including biochemistry, cellular and molecular biology and immunology, resulting in 19 peer-reviewed publications to date (17 original articles and 2 reviews) with over 500 citations in total. During my Masters in the Vectors and Gene Therapy Group at CNC (Coimbra), I worked on targeted multimodal strategies combining chemotherapy and gene modulation for the treatment of glioblastoma. During my PhD at the University of Cambridge and the Karolinska Institutet, I worked on projects on immune cell biology, immunometabolism and immunotherapy. My work resulted in 3 original first-author and 1 co-first author publications, including one describing a novel strategy based on the fine-tuning of oxygen levels to boost T cell- based immunotherapy (eLife) and another describing the previously unknown role of T cell intrinsic nitric oxide in facilitating tumour infiltration (Cancer Immunology Research). During my career of more than 10 years as a researcher in 3 different laboratories across 3 countries, I have engaged in several collaborative projects. These collaborations resulted in 7 original second-author manuscripts, including one describing the role of glutarate as a new immunometabolite in T cells (Nature Metabolism) and another employing a targeted delivery of nucleic acids to glioblastoma using lipid-based nanoparticles (Journal of Controlled Release). I secured funding from the FCT (Portuguese Fundação para a Ciência e Tecnologia) to conduct my graduate studies outside of Portugal. I also attended and presented at more than 5 international scientific conferences, having won two best Poster presentation prizes on two occasions. To pursue my career path in innovative immunology-related projects, after my PhD, in 2022, I joined the laboratory of Dr. Filipe Pereira (CNC, Coimbra), who is at the forefront of using direct cellular reprogramming to generate rare subsets of immune cells with therapeutic potential, as recently published in Science (DOI: 10.1126/science.adn9083). Since joining the Pereira lab, I set out to understand the molecular determinants of dendritic cell reprogramming. By combining my knowledge of genetic manipulation, metabolism, and immunotherapy, I have collected preliminary data that will lead to the improvement of a new immunotherapy modality based on dendritic cell reprogramming.
Identification

Personal identification

Full name
Pedro Pacheco Cunha

Citation names

  • Pedro P. Cunha

Author identifiers

Ciência ID
861B-C9D3-9F08
ORCID iD
0000-0002-3814-6289

Email addresses

  • pedrocunha@cnc.uc.pt (Professional)
  • pedrocunha37@gmail.com (Personal)

Addresses

  • UcBiotech - Parque Biotecnologico de Cantanhede, Lote 8, 3060-197, Cantanhede, Coimbra, Portugal (Professional)

Knowledge fields

  • Medical and Health Sciences - Basic Medicine - Immunology

Languages

Language Speaking Reading Writing Listening Peer-review
English Proficiency (C2) Proficiency (C2) Proficiency (C2) Proficiency (C2) Proficiency (C2)
Portuguese (Mother tongue)
Education
Degree Classification
2017 - 2022
Concluded
Development, Physiology and Neuroscience (Doctor of Philosophy)
University of Cambridge Queens' College, United Kingdom
"The role of hypoxia in T cell function and immunotherapy" (THESIS/DISSERTATION)
2013 - 2015
Concluded
Biologia Celular e Molecular (Mestrado integrado)
Universidade de Coimbra, Portugal
"On the development of a novel targeted miRNA-based therapy towards glioblastoma" (THESIS/DISSERTATION)
19
2010 - 2013
Concluded
Bioquímica (Licenciatura)
Universidade de Coimbra, Portugal
16
Affiliation

Science

Category
Host institution
Employer
2023/03/29 - Current Postdoc (Research) Universidade de Coimbra, Portugal
Universidade de Coimbra Centro de Inovação em Biomedicina e Biotecnologia, Portugal
2022/04 - 2023/01/31 Contracted Researcher (Research) University of Cambridge Queens' College, United Kingdom
2019/03 - 2022/12 Visiting Researcher (Research) Karolinska Institutet, Sweden
2016/07 - 2017/08 Contracted Researcher (Research) Universidade de Coimbra Centro de Inovação em Biomedicina e Biotecnologia, Portugal

Others

Category
Host institution
Employer
2017/09 - 2022/03 PhD student University of Cambridge Queens' College, United Kingdom
2014/08 - 2016/08 Master Student Universidade de Coimbra Centro de Inovação em Biomedicina e Biotecnologia, Portugal
2012/01 - 2014/06 Bachelor Student Universidade de Coimbra Centro de Inovação em Biomedicina e Biotecnologia, Portugal
Projects

Grant

Designation Funders
2023/02/14 - Current CInTech: Technological Hub for Innovation, Translation and Industrialization of Complex Injectable Drugs
Post-doc
Universidade de Coimbra Centro de Inovação em Biomedicina e Biotecnologia, Portugal
Ongoing
2022/04 - 2022/12 Hypoxia, HIF function and immunotherapy
CAN2018/808
Invited Scientist Fellow
Karolinska Institutet, Sweden
Cancerfonden
Ongoing
2017/09 - 2022/12 Oxygen and the immune response
214283/Z/18/Z
Research Fellow
University of Cambridge Queens' College, United Kingdom
Wellcome Foundation Ltd
Ongoing
2017 - 2022 The role of hypoxia in T cell function and Imunotherapy
SFRH/BD/115612/2016
PhD Student Fellow
University of Cambridge Queens' College, United Kingdom
Fundação para a Ciência e a Tecnologia
Concluded
2017/01 - 2017/08 Differentiation therapy toward glioblastoma stem-like cells
POCI-01-0145- FEDER-016390
Research Fellow
Universidade de Coimbra Centro de Inovação em Biomedicina e Biotecnologia, Portugal
Autoridade de Gestão do Programa Operacional Competitividade e Internacionalização
Concluded
Outputs

Publications

Book chapter
  1. Cardoso, A.M.; Guedes, J.R.; Cardoso, A.L.; Morais, C.; Cunha, P.; Viegas, A.T.; Costa, R.; Jurado, A.; Pedroso de Lima, M.C.. "Recent Trends in Nanotechnology Toward CNS Diseases: Lipid-Based Nanoparticles and Exosomes for Targeted Therapeutic Delivery". 1-40. 2016.
    10.1016/bs.irn.2016.05.002
Conference poster
  1. Morais, Catarina; Cunha, Pedro; Cardoso, A.M.; et al. "Membrane perturbations induced by acylated S4(13)-PV analogs: relevance to nucleic acid delivery". Paper presented in 34th European Peptide Symposium, 2016.
  2. Cunha, Pedro; Costa, Pedro; Costa, Raquel; Custódia, Carlos; Branco, Ruben; Morais, Catarina; Cardoso, A.M.; et al. "MicroRNA modulation renders glioblastoma cells sensitive to chemotherapy". Paper presented in 49th Annual Scientific Meeting of the European Society for Clinical Investigation, 2015.
  3. Cunha, Pedro; Costa, Raquel; Costa, Pedro; Cardoso, A.M.; et al. "A promising targeted strategy to overcome drug-resistance in glioblastoma". Paper presented in EMBO workshop: Modern DNA concepts and tools for safe gene transfer and modification, 2015.
  4. Cunha, Pedro; Costa, Pedro; Costa, Raquel; Branco, Ruben; Morais, Catarina; Cardoso, A.M.; et al. "MicroRNA-21 and micro RNA-128 modulation sensitizes glioblastoma cells to sunitinib treatement". Paper presented in XVIII Congress of the Portuguese Biochemical Society, 2014.
Journal article
  1. Brennan J Wadsworth; Marina Leiwe; Eleanor A Minogue; Pedro P Cunha; Viktor Engman; Carolin Brombach; Christos Asvestis; et al. "A 2-hydroxybutyrate-mediated feedback loop regulates muscular fatigue". eLife (2024): https://doi.org/10.7554/eLife.92707.2.
    10.7554/eLife.92707.2
  2. Iosifina P. Foskolou; Pedro P. Cunha; Elena Sánchez-López; Eleanor A. Minogue; Benoît P. Nicolet; Aurélie Guislain; Christian Jorgensen; et al. "The two enantiomers of 2-hydroxyglutarate differentially regulate cytotoxic T cell function". Cell Reports (2023): http://dx.doi.org/10.1016/j.celrep.2023.113013.
    10.1016/j.celrep.2023.113013
  3. Eleanor Minogue; Pedro P. Cunha; Brennan Wadsworth; Guinevere L. Grice; shiv kumar Sah-Teli; Rob Hughes; David Bargiela; et al. "Glutarate regulates T cell metabolism and anti-tumour immunity". Nature Metabolism (2023): http://dx.doi.org/10.1038/s42255-023-00855-2.
    10.1038/s42255-023-00855-2
  4. Pedro P Cunha; Eleanor Minogue; Lena CM Krause; Rita M Hess; David Bargiela; Brennan J Wadsworth; Laura Barbieri; et al. "Oxygen levels at the time of activation determine T cell persistence and immunotherapeutic efficacy". eLife (2023): https://doi.org/10.7554/eLife.84280.
    10.7554/eLife.84280
  5. Laura Barbieri; Pedro Veliça; Paulo A. Gameiro; Pedro P. Cunha; Iosifina P. Foskolou; Eric Rullman; David Bargiela; Randall S. Johnson; Helene Rundqvist. "Lactate exposure shapes the metabolic and transcriptomic profile of CD8+ T cells". Frontiers in Immunology 14 (2023): http://dx.doi.org/10.3389/fimmu.2023.1101433.
    10.3389/fimmu.2023.1101433
  6. Pedro P. Cunha; David Bargiela; Eleanor Minogue; Lena C.M. Krause; Laura Barbieri; Carolin Brombach; Milos Gojkovic; et al. "Infiltration of tumors is regulated by T cell-intrinsic nitric oxide synthesis". Cancer Immunology Research (2022): https://doi.org/10.1158/2326-6066.CIR-22-0387.
    10.1158/2326-6066.CIR-22-0387
  7. David Bargiela; Pedro P. Cunha; Pedro Veliça; Iosifina P. Foskolou; Laura Barbieri; Helene Rundqvist; Randall S. Johnson. "Vitamin B6 Metabolism Determines T Cell Anti-Tumor Responses". Frontiers in Immunology 13 (2022): http://dx.doi.org/10.3389/fimmu.2022.837669.
    10.3389/fimmu.2022.837669
  8. Foskolou, I. P.; Cunha, P. P.; Minogue, E.; Nicolet, B.; Guislain, A.; Jorgensen, C.; Zandhuis, N. D.; et al. "The two enantiomers of 2-hydroxyglutarate differentially regulate cytotoxic T cell function". BioRxiv (2022): https://publons.com/wos-op/publon/58339254/.
    10.1101/2022.08.18.504372
  9. Gojkovic, M.; Cunha, P.P.; Darmasaputra, G.S.; Barbieri, L.; Rundqvist, H.; Veliça, P.; Johnson, R.S.. "Oxygen-Mediated Suppression of CD8+ T Cell Proliferation by Macrophages: Role of Pharmacological Inhibitors of HIF Degradation". Frontiers in Immunology 12 (2021): http://www.scopus.com/inward/record.url?eid=2-s2.0-85107036662&partnerID=MN8TOARS.
    10.3389/fimmu.2021.633586
  10. Cardoso, A.M.; Morais, C.M.; Pena, F.; Marante, T.; Cunha, P.P.; Jurado, A.S.; Pedroso de Lima, M.C.. "Differentiation of glioblastoma stem cells promoted by miR-128 or miR-302a overexpression enhances senescence-associated cytotoxicity of axitinib". Human Molecular Genetics 30 3-4 (2021): 160-171. http://www.scopus.com/inward/record.url?eid=2-s2.0-85106069155&partnerID=MN8TOARS.
    10.1093/hmg/ddab011
  11. Matuleviciute, R.; Cunha, P.P.; Johnson, R.S.; Foskolou, I.P.. "Oxygen regulation of TET enzymes". FEBS Journal (2021): http://www.scopus.com/inward/record.url?eid=2-s2.0-85099991597&partnerID=MN8TOARS.
    10.1111/febs.15695
  12. Veliça, P.; Cunha, P.P.; Vojnovic, N.; Foskolou, I.P.; Bargiela, D.; Gojkovic, M.; Rundqvist, H.; Johnson, R.S.. "Modified hypoxia-inducible factor expression in CD8þ T cells increases antitumor efficacy". Cancer Immunology Research 9 4 (2021): 401-414. http://www.scopus.com/inward/record.url?eid=2-s2.0-85104428121&partnerID=MN8TOARS.
    10.1158/2326-6066.CIR-20-0561
  13. Barbieri, L.; Velica, P.; Gameiro, P. A.; Cunha, P. P.; Foskolou, I. P.; Bargiela, D.; Rundqvist, H.; Johnson, Randall S.. "Lactate regulation of activation in CD8+ T cells". BioRxiv (2021): https://publons.com/wos-op/publon/58339252/.
    10.1101/2021.12.14.472728
  14. Iosifina P. Foskolou; Laura Barbieri; Aude Vernet; David Bargiela; Pedro P. Cunha; Pedro Velica; Eunyeong Suh; et al. "The S enantiomer of 2-hydroxyglutarate increases central memory CD8 populations and improves CAR-T therapy outcome". Blood Advances (2020): https://doi.org/10.1182/bloodadvances.2020002309.
    10.1182/bloodadvances.2020002309
  15. Cunha, Pedro. "miRNA-31 Improves Cognition and Abolishes Amyloid-ß Pathology by Targeting APP and BACE1 in an Animal Model of Alzheimer’s Disease". Molecular Therapy - Nucleic Acids (2020): http://dx.doi.org/10.1016/j.omtn.2020.01.010.
    10.1016/j.omtn.2020.01.010
  16. Catarina M Morais; Pedro P Cunha; Tânia Melo; Ana M Cardoso; Pedro Domingues; M Rosário Domingues; Maria C Pedroso de Lima; Amália S Jurado. "Glucosylceramide synthase silencing combined with the receptor tyrosine kinase inhibitor axitinib as a new multimodal strategy for glioblastoma". Human Molecular Genetics (2019): https://doi.org/10.1093/hmg/ddz152.
    10.1093/hmg/ddz152
  17. Morais, C.M.; Cardoso, A.M.; Cunha, P.P.; Aguiar, L.; Vale, N.; Lage, E.; Pinheiro, M.; et al. "Acylation of the S413-PV cell-penetrating peptide as a means of enhancing its capacity to mediate nucleic acid delivery: Relevance of peptide/lipid interactions". Biochimica et Biophysica Acta - Biomembranes 1860 12 (2018): 2619-2634. http://www.scopus.com/inward/record.url?eid=2-s2.0-85054439165&partnerID=MN8TOARS.
    10.1016/j.bbamem.2018.10.002
  18. Pedro P Cunha; Pedro M Costa; Catarina M Morais; Inês R Lopes; Ana M Cardoso; Ana L Cardoso; Miguel Mano; Amália S Jurado; Maria C Pedroso de Lima. "High-throughput screening uncovers miRNAs enhancing glioblastoma cell susceptibility to tyrosine kinase inhibitors". Human Molecular Genetics 26 22 (2017): 4375-4387. https://doi.org/10.1093/hmg/ddx323.
    10.1093/hmg/ddx323
  19. Cunha, Pedro. "MiRNA-21 silencing mediated by tumor-targeted nanoparticles combined with sunitinib: A new multimodal gene therapy approach for glioblastoma". Journal of Controlled Release (2015): http://www.sciencedirect.com/science/article/pii/S0168365915002205.
    http://dx.doi.org/10.1016/j.jconrel.2015.04.002
  20. Cunha, P. P.; Costa, P.; Costa, R.; Custodia, C.; Branco, R.; Morais, C. M.; Cardoso, A. M.; et al. "MicroRNA modulation renders glioblastoma cells sensitive to chemotherapy". European Journal of Clinical Investigation (2015): https://publons.com/wos-op/publon/11264927/.
Preprint
  1. Rundqvist, H.; Veliça, P.; Barbieri, L.; Gameiro, P.A.; Cunha, P.P.; Gojkovic, M.; Bargiela, D.; et al. "Lactate potentiates differentiation and expansion of cytotoxic T cells". 2019. http://dx.doi.org/10.1101/571745.
    10.1101/571745

Other

Other output
  1. Modified Hypoxia Inducible Factor expression in CD8+ T cells increases anti-tumor efficacy. 2020. Pedro Veliça; Pedro Pacheco Cunha; Nikola Vojnovic; Iosifina Petrina Foskolou; David Bargiela; Milos Gojkovic; Helene Rundqvist; Randall Scott Johnson. https://doi.org/10.1101/2020.06.18.159137.
    10.1101/2020.06.18.159137
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2021/07 Endogenous nitric oxide production modulates CD8+ T cell function PDN symposium 2021
(Cambridge, United Kingdom)
2018/03 Understanding the role of Arginase and Nitric oxide synthase in CD8+ T cell function PDN symposium 2018
(Cambridge, United Kingdom)
2016/07 On the development of a novel targeted miRNA-based therapy towards glioblastoma I Gala of Biochemistry
(Porto, Portugal)

Event participation

Activity description
Type of event
Event name
Institution / Organization
2023/03 - 2023/03 XLVIII Annual Meeting of the Portuguese Society for Immunology
Meeting
XLVIII Annual Meeting of the Portuguese Society for Immunology
2022/09 - 2022/09 Tuned hypoxic response in CD8+ T cells improves antitumour function (Poster presentation)
Conference
Keystone Immunometabolism (Colorado, USA)
2021/11 - 2021/11 Tuned hypoxic response in T cells improves antitumour function (Poster presentation)
Workshop
EMBO Workshop - Cancer immunometabolism (Barcelona, Spain)
2020/01 - 2020/01 Endogenous nitric oxide production modulates T cell function (Poster presentation)
Conference
Keystone Hypoxia (Colorado, USA)
2019/03 - 2019/03 Endogenous nitric oxide production modulates T cell function (Poster presentation)
Conference
Keystone Cancer Metastasis (Florance, Italy)
2015/05 - 2015/05 MicroRNA modulation renders glioblastoma cells sensitive to chemotherapy (Poster presentation)
Meeting
49th Annual Scientific Meeting of the European Society for Clinical Investigation (Cluj, Romania)
2015/03 - 2015/03 A promising targeted strategy to overcome drug-resistance in glioblastoma (Poster presentation)
Workshop
EMBO workshop: Modern DNA concepts and tools for safe gene transfer and modification (Paris, France)
2014/12 - 2014/12 Vectorized oligonucleotide delivery renders glioblastoma cells sensitive to chemotherapy (Poster presentation)
Congress
XVIII Congress of the Portuguese Biochemical Society (Coimbra, Portugal)
Distinctions

Award

2021 Best Poster Award
2014 Best Poster Award

Other distinction

2017 PhD research studentship
Fundação para a Ciência e a Tecnologia, Portugal