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Nuno M. S. dos Santos graduated in Aquatic Sciences in 1992 (ICBAS, University of Porto). In October 2000, he received his PhD in fish immunology from the University of Wageningen, The Netherlands, under the supervision of Willem van Muiswinkel (University of Wageningen) and Maria de Sousa (University of Porto) and co-supervision of Jan Rombout (University of Wageningen) and Anthony Ellis (Marine Laboratory of Aberdeen, Scotland). In 2004, he established the Fish Immunology and Vaccinology (FIV) group at Instituto de Biologia Molecular e Celular (IBMC), Universidade do Porto, which he coordinates since then. In 2015, the group became part of Instituto de Investigação e Inovação em Saúde (i3S), Universidade do Porto (https://www.i3s.up.pt/research-group.php?groupid=19). He published 44 articles and 2 section of books, has an H-index of 23 (Scopus) and is the inventor of 3 patents. He was PI of 10 grants (>1,300 keuro), including 2 from industry (>650keuro; Novartis Animal Health), in a total of 18 grants (>3,000 keuro) awarded to the group. He supervised 7 post-docs, 7 PhDs (2 ongoing) and co-supervised 1 PhD, and 13 MSc, in addition to countless undergraduates. He participated in PhD and MSc thesis juries, has taught regularly in PhD and Master's programs, regularly reviewed articles for international scientific journals and is academic editor for PLoS Pathogens. Since 2015 he integrates the scientific committee of ETOX, a biannual workshop on bacterial protein toxins that runs for more than 30 years. In his curriculum Ciência Vitae the most frequent terms in the context of scientific and technological output are: Host-pathogen Interactions; Fish Immunology and Immunopathology; Microbiology; Virulence factors and mechanisms; Bacterial toxins; Cellular and Molecular Biology; Immunology; Antigen Presentation; Cancer; Apoptosis; Aquaculture Recirculating Systems.
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Identification

Personal identification

Full name
Nuno Miguel Simões dos Santos

Citation names

  • dos Santos, Nuno M.S.

Author identifiers

Ciência ID
871C-55E5-E483
ORCID iD
0000-0002-5960-0647

Email addresses

  • nsantos@i3s.up.pt (Professional)

Telephones

Telephone
  • 220408800 Ext.: 6102 (Professional)

Addresses

  • Instituto de Biologia Molecular e Celular. Rua Alfredo Allen 208, 4200-135, Porto, Porto, Portugal (Professional)
  • Instituto de Investigação e Inovação em Saúde (i3S), Rua Alfredo Allen nº 208, 4200-135, Porto, Porto, Portugal (Professional)

Websites

Knowledge fields

  • Natural sciences - Biological Sciences

Languages

Language Speaking Reading Writing Listening Peer-review
English Proficiency (C2) Proficiency (C2) Advanced (C1) Proficiency (C2) Advanced (C1)
Spanish; Castilian Elementary (A2) Advanced (C1) Beginner (A1) Upper intermediate (B2)
Education
Degree Classification
2010
Concluded
 Laboratory Animal Science (Outros)
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2000/10/25
Concluded
 Immunology (Doktor (PhD))
Major in Fish Immunology
Wageningen University and Research Wageningen Institute of Animal Sciences, Netherlands
"Development of Immunity in Sea Bass: A Study Towards Vaccination Against Pseudotuberculosis" (THESIS/DISSERTATION)
1992
Concluded
 Ciências do Meio Aquático (Licenciatura)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
 16
Affiliation

Science

Category
Host institution 		Employer
2023/02/01 - Current Principal Investigator (Research) Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2020/02/01 - 2023/01/31 Principal Investigator (Research) Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
(...)
2015/01/15 - 2020/01/31 Auxiliary Researcher (Research) Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2011/01/15 - 2014/01/14 Auxiliary Researcher (Research) Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2008/01/15 - 2011/01/14 Auxiliary Researcher (Research) Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2004/04/01 - 2007/12/31 Auxiliary Researcher (Research) Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2001/04/01 - 2004/03/31 Auxiliary Researcher (Research) Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
1994/01/01 - 1994/03/30 Research Trainee (Research) Wageningen University and Research Wageningen Institute of Animal Sciences, Netherlands

Other Careers

Category
Host institution 		Employer
1992/08/01 - 1993/12/01 Coordenador Técnico (Assistente Técnico) Sorgal, Sociedade de Óleos e Rações SA, Portugal

Others

Category
Host institution 		Employer
1994/04/01 - 1997/12/01 Consultant Sorgal, Sociedade de Óleos e Rações SA, Portugal
Projects

Contract

Designation Funders
2021/03/28 - Current PhotoVac: T3SS and T5SS in Photobacterium damselae subsp. piscicida: from basic studies to vaccine development (Co-PI)
PTDC/CVT-CVT/7177/2020
Co-Principal Investigator (Co-PI)
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2022/11/01 - 2026/10/31 IGNITION - Improving Green Innovation for the Blue Revolution: new tools and opportunities for a more sustainable animal farming
101084651
Co-Principal Investigator (Co-PI)
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
Ongoing
2022/01/01 - 2024/12/31 JumpIN - HiJacking the peripheral neurons retrograde trafficking machinery to deliver neUroprotective Molecules to sPinal cord InterNeurons
PTDC/BTM-MAT/4156/2021
Researcher
Fundação para a Ciência e a Tecnologia
Ongoing
2018/07/15 - 2022/07/14 Structural and functional characterization of AB toxins targeting antigen-presenting cells and their application as cancer vaccines
PTDC/BIA-MIC/29910/2017
Principal investigator
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2018/07/15 - 2022/07/14 Identification and characterization of virulence factors secreted by Photobacterium damselae piscicida with potential to be used as antigens for vaccination against fish photobacteriosis (Co-PI)
PTDC/CVT-CVT/30018/2017
Co-Principal Investigator (Co-PI)
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal

Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2016/05 - 2019/04 Structured program on bioengineered therapies for infectious diseases and tissue regeneration
Norte-01-0145-FEDER-000012
Principal investigator
Norte2020 Programa Operacional Regional do Norte
2016/01 - 2018/12 Host cell factors and molecular mechanisms implicated in intoxication by AIP56, a bacterial toxin targeting NF-kB
Provided by PTCRIS: 140633
Co-Principal Investigator (Co-PI)
Fundação para a Ciência e a Tecnologia, I.P.
2015/10 - 2018/09 Projecto ALISSA: Alimentação saudável e sustentável para peixes de aquacultura
08/SI/2015
 Agência Nacional de Inovação
2013/12 - 2015/06 Host-Pathogen Interactions: From pathogen virulence factors to host responses, disease induction and therapeutic control
NORTE-07-0124-FEDER-000002
Principal investigator
ON2 Programa Operacional Regional do Norte
2013/05 - 2015/05 Studies towards identification of structural determinants required for receptor binding and membrane translocation of AIP56, a Photobacterium damselae subsp. piscicida apoptogenic AB toxin targeting NF-kB
129800
Provided by PTCRIS: 129800
Researcher
Fundação para a Ciência e a Tecnologia, I.P.
2010/06 - 2013/11 Cell entry and intracellular trafficking of the apoptogenic AIP56 exotoxin, a key virulence factor of Photobacterium damselae subsp. piscicida
99544
Provided by PTCRIS: 99544
Researcher
Fundação para a Ciência e a Tecnologia, I.P.
2010/05 - 2013/10 Rational design of an AIP56-based anti-pasteurellosis vaccine
PTDC/MAR/101143/2008
Principal investigator
Fundação para a Ciência e a Tecnologia, I.P.
2010/04 - 2012/01 Biochemical and biophysical characterization of AIP56, a novel bacterial apoptogenic exotoxin
PTDC/BIA-PRO/101111/2008
Principal investigator
Fundação para a Ciência e a Tecnologia, I.P.
2007/10 - 2011/06 Creating tools for studying sea bass (Dicentrarchus labrax) immunity
PTDC/CVT/69086/2006
Principal investigator
Fundação para a Ciência e a Tecnologia, I.P.
2007/01 - 2009/01 Studies on the apoptosis induced in sea bass (Dicentrarchus labrax L.) phagocytes by Photobacterium damselae subsp. piscicida exotoxin AIP56.
82147
Provided by PTCRIS: 82147
Researcher
Fundação para a Ciência e a Tecnologia, I.P.
2004/04/01 - 2007/12/31 Development of vaccines against pathogens important for warm water aquaculture
Not applicable
Principal investigator
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
 Novartis Animal Vaccines Ltd (UK)
Concluded
2004/12 - 2007/11 Production of important markers for monitoring the immune response in sea bass (Dicentrarchus labrax, L.)
POCTI/CVT/44925/2002
Principal investigator
Fundação para a Ciência e a Tecnologia, I.P.
2005/11 - 2007/03 Studies on the apoptosis induced in sea bass (Dicentrarchus labrax L.) phagocytes by Photobacterium damselae subsp. piscicida exotoxin AIP56.
Provided by PTCRIS: 56111
56111
Researcher
Fundação para a Ciência e a Tecnologia, I.P.
2001/04/01 - 2004/03/01 Development of vaccines against Nodavirus and Photobacterium damselae ssp. piscicida
-
Principal investigator
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
 Aqua Health (Europe) Ltd

Novartis Animal Vaccines Ltd (UK)
Concluded
Outputs

Publications

Book chapter
  1. Barnes, A.C.; Dos Santos, N.M.S.; Ellis, A.E.. "Update on bacterial vaccines: Photobacterium damselae subsp. piscicida". In Fish Vaccinology. 2005.
  2. dos Santos, Nuno M.S.; Taverne, N.; Taverne-Thiele, A.J.; Rombout, J.H.W.M.. "Application and Characterisation of Monoclonal Antibodies against Sea Bass (Dicentrarchus labrax L.) IgM". In Fish Vaccinology, edited by Gudding, R.; Lillehaug, A.; Midtlyng, P.J, 469. Basel, Switzerland: Karger, 1997.
    Published
Conference poster
  1. dos Santos, Nuno M.S.. "Three-dimensional structure and mechanisms of membrane interaction and translocation of the NF¿B-targeting toxin AIP56". Paper presented in ETOX21, European Workshop on Bacterial Protein Toxins, 2023.
  2. dos Santos, Nuno M.S.. "Development of tools towards the use of AIP56 as a vehicle for the intracellular delivery of antigens in antigen-presenting cells". Paper presented in ETOX21, European Workshop on Bacterial Protein Toxins, 2023.
  3. dos Santos, Nuno M.S.. "The two-component system RstAB regulates toxin expression in Photobacterium damselae subsp. piscicida". Paper presented in ETOX21, European Workshop on Bacterial Protein Toxins, 2023.
  4. dos Santos, Nuno M.S.. "Structural and functional characterization of NiTox: a new AB-toxin from Vibrio Nigripulchritudo lethal to shrimp". Paper presented in ETOX21, European Workshop on Bacterial Protein Toxins, 2021.
  5. dos Santos, Nuno M.S.. "Outer Membrane Vesicles from Photobacterium damselae subsp. piscicida: characterization and antigenic potential". Paper presented in Annual Meeting ISEV, 2020.
  6. dos Santos, Nuno M.S.; Lisboa, Johnny; Fernandes, Andreia; Freitas, Inês Lua; do Vale, Ana. "Structural Insights on AIP56 and AIP56-relarted Toxins". Paper presented in ETOX19, European Workshop on Bacterial protein Toxins, Davos, Switzerland, 22-26 June 2019, 2019.
  7. dos Santos, Nuno M.S.; do Vale, Ana; Osorio, Carlos Rodriguez; Pereira, Cassilda. "The type II secretion of Photobacterium damselae piscicida mediates extracellular release of the apoptogenic toxin AIP56". Paper presented in ETOX 18, 18th European Workshop on Bacterial Protein Toxins, Paris, France, 26-30 June, 2017, 2017.
  8. dos Santos, Nuno M.S.; Lisboa, Johnny; Rodrigues, Inês Salvador; Benz, Roland; do Vale, Ana. "Structural determinants required for pH-induced unfolding and pore formation in AIP56". Paper presented in ETOX 18, 18th European Workshop on Bacterial Protein Toxins, Paris, France, 26-30 June, 2017, 2017.
  9. dos Santos, Nuno M.S.; Rodrigues, Inês Salvador; Pereira, Cassilda; Pereira, Liliana Marisa Gonçalves; Lisboa, Johnny; do Vale, Ana. "Host factors involved in AIP56 intoxication: the role of protein chaperones and redox systems". Paper presented in ETOX 18, 18th European Workshop on Bacterial Protein Toxins, Paris, France, 26-30 June, 2017, 2017.
  10. dos Santos, Nuno M.S.; Peres, José Martinho Oliveira; Rodrigues, Inês Salvador; Pereira, Liliana Marisa Gonçalves; Pinto, Rute Daniela Pinheiro da Silva; Lisboa, Johnny; do Vale, Ana. "AIP56: a short-trip AB toxin with a peculiar translocation domain". Paper presented in ETOX 17, 17th European Workshop on Bacterial Protein Toxins, Braga, Portugal, 20-24 June, 2015, 2015.
  11. dos Santos, Nuno M.S.; Pereira, Liliana Marisa Gonçalves; Pinto, Rute Daniela Pinheiro da Silva; Peres, José Martinho; Rodrigues, Inês Salvador; Lisboa, Johnny; do Vale, Ana. "Involvement of Hsp90 and PPIases in AIP56 intoxication". Paper presented in ETOX 17, 17th European Workshop on Bacterial Protein Toxins, Braga, Portugal, 20-24 June, 2015, 2015.
  12. dos Santos, Nuno M.S.; Silva, Daniela Sá Pereira; Pereira, Liliana Marisa Gonçalves; Moreira, Ana Raquel; Araújo, Leonor; do Vale, Ana. "AIP56 and NleC: structural and functional conservation but different unfolding properties". Paper presented in ETOX 16, 16th European Workshop on Bacterial Protein Toxins, Freiburg, Germany, 22-26 June 2013, 2013.
  13. dos Santos, Nuno M.S.; Pereira, Liliana Marisa Gonçalves; Silva, Daniela Sá Pereira; Moreira, Ana Raquel; Sampaio, Paula; do Vale, Ana. "Trafficking of AIP56 in mouse bone marrow-derived macrophages". Paper presented in ETOX 16, 16th European Workshop on Bacterial Protein Toxins, Freiburg, Germany, 22-26 June 2013, 2013.
    10.1164/ajrccm-conference.2011.183.1_meetingabstracts.a1076
  14. dos Santos, Nuno M.S.; Silva, Daniela Sá Pereira; Pereira, Liliana Marisa Gonçalves; Silva, Frederico; do Vale, Ana. "AIP56, an apoptogenic AB-toxin targeting NF-kB". Paper presented in ETOX15, 15th European Workshop on Bacterial Protein toxins, Oslo, Norway, June 18-22, 2011, 2011.
  15. dos Santos, Nuno M.S.; Pereira, Liliana Marisa Gonçalves; Silva, Daniela Sá Pereira; Beitzinger, Christoph; Benz, Roland; do Vale, Ana. "Studies on the intracelular trafficking of the apoptogenic toxin AIP56". Paper presented in ETOX15, 15th European Workshop on Bacterial Protein toxins, Oslo, Norway, June 18-22, 2011, 2011.
  16. dos Santos, Nuno M.S.; Pinto, Rute Daniela Pinheiro da Silva; Pereira, Pedro J.B.. "Class I antigen presentation in sea bass (Dicentrarchus labrax, L): identification of key peptide loading complex genes". Paper presented in First EOFFI (Euroepean Organisation of Fish Immunology) symposium, P44, Domus La Quercia, Viterbo, Italy. May 23-27, 2010, 2010.
  17. dos Santos, Nuno M.S.; Silva, Daniela Sá Pereira; Ferreira-da-Silva, Frederico; do Vale, Ana. "Structural and functional studies on AIP56, a novel bacterial apoptogenic toxin". Paper presented in ETOX14, 14th European Workshop on Bacterial Protein toxins, Obernai, France, June 27-July 2, 2009, 2009.
  18. dos Santos, Nuno M.S.; Pereira, Liliana Marisa Gonçalves; Costa-Ramos, Carolina; do Vale, Ana. "Studies on the interaction of AIP56, a novel bacterial apoptogenic exotoxin, with host cells". Paper presented in ETOX14, 14th European Workshop on Bacterial Protein toxins, Obernai, France, June 27-July 2, 2009, 2009.
  19. dos Santos, Nuno M.S.; do Vale, Ana; Costa-Ramos, Carolina; Pereira, Liliana Marisa Gonçalves; Silva, Manuel Teixeira. "Phagocyte apoptosis induced by the bacterial exotoxin AIP56 involves toxin internalization and the participation of mitochondria". Paper presented in 7th international euroconference on mechanisms of cell death and disease: advances in therapeutic intervention and drug development, Cascais, Lisboa, Portugal, 30 October-4 November, 2008, 2008.
  20. dos Santos, Nuno M.S.; Costa-Ramos, Carolina; do Vale, Ana; Silva, Manuel T.. "Studies on apoptosis induced by AIP56, a novel virulence factor of Photobacterium damselae ssp piscicida". Paper presented in 16th Euroconference on Apoptosis and 5th Swiss Apoptosis Meeting, Bern, Switzerland, September 6-9, 2008, 2008.
  21. dos Santos, Nuno M.S.; Reis, Marta Inês Rocha; do Vale, Ana. "Molecular cloning and characterisation of sea bass (Dicentrarchus labrax) caspase-8". Paper presented in 16th Euroconference on Apoptosis and 5th Swiss Apoptosis Meeting, Bern, Switzerland, September 6-9, 2008, 2008.
  22. dos Santos, Nuno M.S.; Silva, Daniela S.P.; Reis, Marta Inês Rocha; Nascimento, Diana (0F14-BB9C-AFC3); do Vale, Ana; Pereira, Pedro J.B.. "Sea bass (Dicentrarchus labrax) invariant chain and class II major histocompatibility complex: sequencing and structural analysis using 3D homology modelling". Paper presented in 7th Nordic Symposium on Fish Immunology, Stirling, Scotland, UK, 19-22 June 2007, 2007.
  23. dos Santos, Nuno M.S.; do Vale, Ana; Costa-Ramos, Carolina; Silva, Daniela S.P.; Macedo, Paula; Fernandes, Rui; Sampaio, Paula; Silva, Manuel T.. "Brush border microvillar loss during detachment-triggered apoptosis (anoikis) of enterocytes". Paper presented in XLI Congresso of the Portuguese Society for Microscopy, Braga, Portugal, 14-15 December, 2006, 2006.
  24. dos Santos, Nuno M.S.; Nascimento, Diana (0F14-BB9C-AFC3); do Vale, Ana; Tomas, Ana M.; Zou, Jun; Secombes, Christopher J.. "Cloning, promoter analysis and expression of sea bass (Dicentrarchus labrax L.) interleukine-12 p40 and p35 subunits". Paper presented in Annual Meeting of the Sociedade Portuguesa de Imunologia, Innate and acquired immunity: evolution and regulation, Braga, Portugal, 13-14 October, 2006, 2006.
  25. dos Santos, Nuno M.S.; Nascimento, Diana (0F14-BB9C-AFC3); do Vale, Ana; Secombes, Christopher J.. "Molecular characterisation and production of sea bass (Dicentrarchus labrax L.) recombinant tumour necrosis factor alpha (TNF-a)". Paper presented in 12th International Conference of the EAFP, Copenhagen, Denmark, 11-16 September, 2005, 2005.
  26. dos Santos, Nuno M.S.; Reis, Marta Inês Rocha; do Vale, Ana. "Molecular cloning and characterisation of sea bass (Dicentrarchus labrax L.) caspase-1, -3 and -9 genes". Paper presented in 12th International Conference of the EAFP, Copenhagen, Denmark, 11-16 September, 2005, 2005.
  27. dos Santos, Nuno M.S.; Pinto, Rute Daniela Pinheiro da Silva; Nascimento, Diana (0F14-BB9C-AFC3). "Molecular cloning and characterisation of sea bass (Dicentrarchus labrax L.) CD8alpha". Paper presented in 12th International Conference of the EAFP, Copenhagen, Denmark, 11-16 September, 2005, 2005.
  28. dos Santos, Nuno M.S.; do Vale, Ana; Sousa, Maria João; Silva, Manuel T.. "Mycobactrium marinum infection in farmed turbot (Scophtalmus maximus)". Paper presented in 10th International Conference of the EAFP, Trinity College – Dublin, Ireland, 9-14 September, 2001., 2001.
  29. dos Santos, Nuno M.S.; Hermsen, Trudy; Pilstrom, Lars; Stet, René. "Characterisation of the Immunoglobulin light chain of sea bass (Dicentrarchus labrax) suggests possible low diversity of the V regions". Paper presented in 8th congress of the International Society of Developmental and Comparative immunology, Cairns, Australia, 2-6 July 2000, 2000.
Journal article
  1. Lisboa, Johnny; Pereira, Cassilda; Pinto, Rute D.; Rodrigues, Inês S.; Pereira, Liliana M. G.; Pinheiro, Bruno; Oliveira, Pedro; et al. "Unconventional structure and mechanisms for membrane interaction and translocation of the NF-¿B-targeting toxin AIP56". Nature Communications 14 1 (2023): http://dx.doi.org/10.1038/s41467-023-43054-z.
    Published • 10.1038/s41467-023-43054-z
  2. Alexandra Teixeira; Inês Loureiro; Johnny Lisboa; Pedro N. Oliveira; Jorge E. Azevedo; Nuno M. S. dos Santos; Ana do Vale. "Characterization and Vaccine Potential of Outer Membrane Vesicles from Photobacterium damselae subsp. piscicida". International Journal of Molecular Sciences (2023): https://doi.org/10.3390/ijms24065138.
    10.3390/ijms24065138
  3. Inês Lua Freitas; Alexandra Teixeira; Inês Loureiro; Johnny Lisboa; Aurélia Saraiva; Nuno Miguel Simões dos Santos; Ana do Vale. "Susceptibility of Sea Bream (Sparus aurata) to AIP56, an AB-Type Toxin Secreted by Photobacterium damselae subsp. piscicida". Toxins 14 2 (2022): 119-119. https://doi.org/10.3390/toxins14020119.
    10.3390/toxins14020119
  4. Lisboa, Johnny; Pereira, Cassilda; Rifflet, Aline; Ayala, Juan; Terceti, Mateus S.; Barca, Alba V.; Rodrigues, Inês; et al. "A Secreted NlpC/P60 Endopeptidase from Photobacterium damselae subsp. piscicida Cleaves the Peptidoglycan of Potentially Competing Bacteria". mSphere 6 1 (2021): http://dx.doi.org/10.1128/msphere.00736-20.
    10.1128/msphere.00736-20
  5. Inês S. Rodrigues; Liliana M. G. pereira; Johnny Lisboa; Cassilda Pereira; Pedro Oliveira; Nuno M. S. dos Santos; Ana do Vale. "Author Correction: Involvement of Hsp90 and cyclophilins in intoxication by AIP56, a metalloprotease toxin from Photobacterium damselae subsp. piscicida". Scientific Reports (2020): https://doi.org/10.1038/s41598-020-58152-x.
    10.1038/s41598-020-58152-x
  6. Inês S. Rodrigues; Liliana M. G. Pereira; Johnny Lisboa; Cassilda Pereira; Pedro Oliveira; Nuno M. S. dos Santos; Ana do Vale. "Involvement of Hsp90 and cyclophilins in intoxication by AIP56, a metalloprotease toxin from Photobacterium damselae subsp. piscicida". Scientific Reports 9 1 (2019): https://doi.org/10.1038/s41598-019-45240-w.
    10.1038/s41598-019-45240-w
  7. Abushattal, S.; Vences, A.; Dos Santos, N.M.S.; Do Vale, A.; Osorio, C.R.. "Draft genome sequences of photobacterium damselae subsp. Piscicida snw-8.1 and pp3, two fish-isolated strains containing a type III secretion system". Microbiology Resource Announcements 8 21 (2019): http://www.scopus.com/inward/record.url?eid=2-s2.0-85067227514&partnerID=MN8TOARS.
    10.1128/MRA.00426-19
  8. Terceti, M.S.; Vences, A.; Matanza, X.M.; Barca, A.V.; Noia, M.; Lisboa, J.; Dos Santos, N.M.S.; Do Vale, A.; Osorio, C.R.. "The RstAB system impacts virulence, motility, cell morphology, penicillin tolerance and production of type II secretion system-dependent factors in the fish and human pathogen photobacterium damselae subsp. damselae". Frontiers in Microbiology 10 APR (2019): http://www.scopus.com/inward/record.url?eid=2-s2.0-85068109857&partnerID=MN8TOARS.
    10.3389/fmicb.2019.00897
  9. Pereira, C.; Rodrigues, I.S.; Pereira, L.M.G.; Lisboa, J.; Pinto, R.D.; Araújo, L.; Oliveira, P.; et al. "Role of AIP56 disulphide bond and its reduction by cytosolic redox systems for efficient intoxication". Cellular Microbiology (2019): http://www.scopus.com/inward/record.url?eid=2-s2.0-85073780861&partnerID=MN8TOARS.
    10.1111/cmi.13109
  10. Pinheiro, J.; Lisboa, J.; Pombinho, R.; Carvalho, F.; Carreaux, A.; Brito, C.; Pöntinen, A.; et al. "MouR controls the expression of the Listeria monocytogenes Agr system and mediates virulence". Nucleic acids research 46 18 (2018): 9338-9352. http://www.scopus.com/inward/record.url?eid=2-s2.0-85054896319&partnerID=MN8TOARS.
    10.1093/nar/gky624
  11. do Vale, A.; Pereira, C.; Osorio, C.R.; dos Santos, N.M.S.. "The apoptogenic toxin aip56 is secreted by the type ii secretion system of photobacterium damselae subsp. Piscicida". Toxins 9 11 (2017): http://www.scopus.com/inward/record.url?eid=2-s2.0-85034449974&partnerID=MN8TOARS.
    10.3390/toxins9110368
  12. Pereira, L.M.G.; Pinto, R.D.; Silva, D.S.; Moreira, A.R.; Beitzinger, C.; Oliveira, P.; Sampaio, P.; et al. "Intracellular trafficking of AIP56, an NF-¿B-cleaving toxin from Photobacterium damselae subsp. piscicida". Infection and Immunity 82 12 (2014): 5270-5285. http://www.scopus.com/inward/record.url?eid=2-s2.0-84911433340&partnerID=MN8TOARS.
    10.1128/IAI.02623-14
  13. Pinto, R.D.; Moreira, A.R.; Pereira, P.J.B.; dos Santos, N.M.S.. "Molecular cloning and characterization of sea bass (Dicentrarchus labrax, L.) calreticulin". Fish and Shellfish Immunology 34 6 (2013): 1611-1618. http://www.scopus.com/inward/record.url?eid=2-s2.0-84877621851&partnerID=MN8TOARS.
    10.1016/j.fsi.2013.03.004
  14. Pinto, R.D.; Moreira, A.R.; Pereira, P.J.B.; Dos Santos, N.M.S.. "Two thioredoxin-superfamily members from sea bass (Dicentrarchus labrax, L.): Characterization of PDI (PDIA1) and ERp57 (PDIA3)". Fish and Shellfish Immunology 35 4 (2013): 1163-1175. http://www.scopus.com/inward/record.url?eid=2-s2.0-84883552448&partnerID=MN8TOARS.
    10.1016/j.fsi.2013.07.024
  15. Pinto, R.D.; Randelli, E.; Buonocore, F.; Pereira, P.J.B.; dos Santos, N.M.S.. "Molecular cloning and characterization of sea bass (Dicentrarchus labrax, L.) MHC class I heavy chain and ß2-microglobulin". Developmental and Comparative Immunology 39 3 (2013): 234-254. http://www.scopus.com/inward/record.url?eid=2-s2.0-84873450024&partnerID=MN8TOARS.
    10.1016/j.dci.2012.10.002
  16. Silva, D.S.; Pereira, L.M.G.; Moreira, A.R.; Ferreira-da-Silva, F.; Brito, R.M.; Faria, T.Q.; Zornetta, I.; et al. "The Apoptogenic Toxin AIP56 Is a Metalloprotease A-B Toxin that Cleaves NF-¿b P65". PLoS Pathogens 9 2 (2013): http://www.scopus.com/inward/record.url?eid=2-s2.0-84880954175&partnerID=MN8TOARS.
    10.1371/journal.ppat.1003128
  17. Reis, M.I.R.; do Vale, A.; Pereira, P.J.B.; Azevedo, J.E.; dos Santos, N.M.S.. "Caspase-1 and IL-1ß Processing in a Teleost Fish". PLoS ONE 7 11 (2012): http://www.scopus.com/inward/record.url?eid=2-s2.0-84870595247&partnerID=MN8TOARS.
    10.1371/journal.pone.0050450
  18. Pinto, R.D.; da Silva, D.V.; Pereira, P.J.B.; dos Santos, N.M.S.. "Molecular cloning and characterization of sea bass (Dicentrarchus labrax, L.) Tapasin". Fish and Shellfish Immunology 32 1 (2012): 110-120. http://www.scopus.com/inward/record.url?eid=2-s2.0-84859241049&partnerID=MN8TOARS.
    10.1016/j.fsi.2011.10.029
  19. Pinto, R.D.; Pereira, P.J.B.; dos Santos, N.M.S.. "Transporters associated with antigen processing (TAP) in sea bass (Dicentrarchus labrax, L.): Molecular cloning and characterization of TAP1 and TAP2". Developmental and Comparative Immunology 35 11 (2011): http://www.scopus.com/inward/record.url?eid=2-s2.0-80053309062&partnerID=MN8TOARS.
    10.1016/j.dci.2011.03.024
  20. Costa-Ramos, C.; Vale, A.D.; Ludovico, P.; dos Santos, N.M.S.; Silva, M.T.. "The bacterial exotoxin AIP56 induces fish macrophage and neutrophil apoptosis using mechanisms of the extrinsic and intrinsic pathways". Fish and Shellfish Immunology 30 1 (2011): 173-181. http://www.scopus.com/inward/record.url?eid=2-s2.0-78650697665&partnerID=MN8TOARS.
    10.1016/j.fsi.2010.10.007
  21. Silva, M.T.; dos Santos, N.M.S.; do Vale, A.. "AIP56: A novel bacterial apoptogenic toxin". Toxins 2 4 (2010): 905-918. http://www.scopus.com/inward/record.url?eid=2-s2.0-78649552139&partnerID=MN8TOARS.
    10.3390/toxins2040905
  22. Reis, M.I.R.; Costa-Ramos, C.; do Vale, A.; dos Santos, N.M.S.. "Molecular cloning of sea bass (Dicentrarchus labrax L.) caspase-8 gene and its involvement in Photobacterium damselae ssp. piscicida triggered apoptosis". Fish and Shellfish Immunology 29 1 (2010): 58-65. http://www.scopus.com/inward/record.url?eid=2-s2.0-77955057057&partnerID=MN8TOARS.
    10.1016/j.fsi.2010.02.016
  23. Monteiro, Sandra M.; Dos Santos, Nuno M. S.; Calejo, Margarida; Fontainhas-Fernandes, Antonio; Sousa, Mario. "The effect of copper toxicity in apoptotic response and cell proliferation, in gills of Oreochromis niloticus". Comparative Biochemistry and Physiology a-Molecular & Integrative Physiology 153A 2 (2009): S63-S63.
    10.1016/j.cbpa.2009.04.537
  24. Monteiro, S.M.; dos Santos, N.M.S.; Calejo, M.; Fontainhas-Fernandes, A.; Sousa, M.. "Copper toxicity in gills of the teleost fish, Oreochromis niloticus: Effects in apoptosis induction and cell proliferation". Aquatic Toxicology 94 3 (2009): 219-228. http://www.scopus.com/inward/record.url?eid=2-s2.0-69249213355&partnerID=MN8TOARS.
    10.1016/j.aquatox.2009.07.008
  25. Silva, M.T.; do Vale, A.; dos Santos, N.M.S.. "Fish and apoptosis: Studies in disease and pharmaceutical design". Current Pharmaceutical Design 14 2 (2008): 170-183. http://www.scopus.com/inward/record.url?eid=2-s2.0-38949102730&partnerID=MN8TOARS.
    10.2174/138161208783378734
  26. dos Santos, N.M.S.; do Vale, A.; Reis, M.I.R.; Silva, M.T.. "Fish and apoptosis: Molecules and pathways". Current Pharmaceutical Design 14 2 (2008): 148-169. http://www.scopus.com/inward/record.url?eid=2-s2.0-38949175453&partnerID=MN8TOARS.
    10.2174/138161208783378743
  27. Silva, M.T.; Do Vale, A.; Dos Santos, N.M.N.. "Secondary necrosis in multicellular animals: An outcome of apoptosis with pathogenic implications". Apoptosis 13 4 (2008): 463-482. http://www.scopus.com/inward/record.url?eid=2-s2.0-41149115987&partnerID=MN8TOARS.
    10.1007/s10495-008-0187-8
  28. Reis, M.I.R.; Nascimento, D.S.; do Vale, A.; Silva, M.T.; dos Santos, N.M.S.. "Molecular cloning and characterisation of sea bass (Dicentrarchus labrax L.) caspase-3 gene". Molecular Immunology 44 5 (2007): 774-783. http://www.scopus.com/inward/record.url?eid=2-s2.0-33748785440&partnerID=MN8TOARS.
    10.1016/j.molimm.2006.04.028
  29. Pinto, R.D.; Nascimento, D.S.; Reis, M.I.R.; do Vale, A.; dos Santos, N.M.S.. "Molecular characterization, 3D modelling and expression analysis of sea bass (Dicentrarchus labrax L.) interleukin-10". Molecular Immunology 44 8 (2007): 2066-2075. http://www.scopus.com/inward/record.url?eid=2-s2.0-34147189842&partnerID=MN8TOARS.
    10.1016/j.molimm.2006.09.008
  30. Do Vale, A.; Costa-Ramos, C.; Silva, D.S.P.; Macedo, P.M.; Fernandes, R.; Sampaio, P.; Dos Santos, N.M.S.; Silva, M.T.. "Cytochemical and ultrastructural study of anoikis and secondary necrosis in enterocytes detached in vivo". Apoptosis 12 6 (2007): 1069-1083. http://www.scopus.com/inward/record.url?eid=2-s2.0-34247889986&partnerID=MN8TOARS.
    10.1007/s10495-006-0040-x
  31. do Vale, A.; Costa-Ramos, C.; Silva, A.; Silva, D.S.P.; Gärtner, F.; dos Santos, N.M.S.; Silva, M.T.. "Systemic macrophage and neutrophil destruction by secondary necrosis induced by a bacterial exotoxin in a Gram-negative septicaemia". Cellular Microbiology 9 4 (2007): 988-1003. http://www.scopus.com/inward/record.url?eid=2-s2.0-33947168734&partnerID=MN8TOARS.
    10.1111/j.1462-5822.2006.00846.x
  32. Reis, M.I.R.; do Vale, A.; Pinto, C.; Nascimento, D.S.; Costa-Ramos, C.; Silva, D.S.P.; Silva, M.T.; et al. "First molecular cloning and characterisation of caspase-9 gene in fish and its involvement in a gram negative septicaemia". Molecular Immunology 44 7 (2007): 1765-1775. http://www.scopus.com/inward/record.url?eid=2-s2.0-33751258003&partnerID=MN8TOARS.
    10.1016/j.molimm.2006.07.301
  33. Nascimento, D.S.; Pereira, P.J.B.; Reis, M.I.R.; do Vale, A.; Zou, J.; Silva, M.T.; Secombes, C.J.; dos Santos, N.M.S.. "Molecular cloning and expression analysis of sea bass (Dicentrarchus labrax L.) tumor necrosis factor-a (TNF-a)". Fish and Shellfish Immunology 23 3 (2007): 701-710. http://www.scopus.com/inward/record.url?eid=2-s2.0-34447279908&partnerID=MN8TOARS.
    10.1016/j.fsi.2007.02.003
  34. Silva, D.S.P.; Reis, M.I.R.; Nascimento, D.S.; Vale, A.d.; Pereira, P.J.B.; dos Santos, N.M.S.. "Sea bass (Dicentrarchus labrax) invariant chain and class II major histocompatibility complex: Sequencing and structural analysis using 3D homology modelling". Molecular Immunology 44 15 (2007): 3758-3776. http://www.scopus.com/inward/record.url?eid=2-s2.0-34249286269&partnerID=MN8TOARS.
    10.1016/j.molimm.2007.03.025
  35. Nascimento, Diana S.; do Vale, Ana; Tomas, Ana M.; Zou, Jun; Secombes, Christopher J.; dos Santos, Nuno M. S.. "Cloning, promoter analysis and expression in response to bacterial exposure of sea bass (Dicentrarchus labrax L.) interleukin-12 p40 and p35 subunits". Molecular Immunology 44 9 (2007): 2277-2291.
    10.1016/j.molimm.2006.11.006
  36. Pinto, R.D.; Nascimento, D.S.; Vale, A.D.; Santos, N.M.S.D.. "Molecular cloning and characterization of sea bass (Dicentrarchus labrax L.) CD8a". Veterinary Immunology and Immunopathology 110 1-2 (2006): 169-177. http://www.scopus.com/inward/record.url?eid=2-s2.0-32044440076&partnerID=MN8TOARS.
    10.1016/j.vetimm.2005.11.005
  37. Do Vale, A.; Silva, M.T.; Dos Santos, N.M.S.; Nascimento, D.S.; Reis-Rodrigues, P.; Costa-Ramos, C.; Ellis, A.E.; Azevedo, J.E.. "AIP56, a novel plasmid-encoded virulence factor of Photobacterium damselae subsp. piscicida with apoptogenic activity against sea bass macrophages and neutrophils". Molecular Microbiology 58 4 (2005): 1025-1038. http://www.scopus.com/inward/record.url?eid=2-s2.0-27944453406&partnerID=MN8TOARS.
    10.1111/j.1365-2958.2005.04893.x
  38. Dos Santos, N.M.S.; Do Vale, A.; Sousa, M.J.; Silva, M.T.. "Mycobacterial infection in farmed turbot Scophthalmus maximus". Diseases of Aquatic Organisms 52 1 (2002): 87-91. http://www.scopus.com/inward/record.url?eid=2-s2.0-0037038713&partnerID=MN8TOARS.
  39. Dos Santos, N.M.S.; Hermsen, T.; Rombout, J.H.W.M.; Pilström, L.; Stet, R.J.M.. "Ig light chain variability in DNP494-KLH immunised sea bass (Dicentrarchus labrax L.): Evidence for intra-molecular induced suppression". Developmental and Comparative Immunology 25 5-6 (2001): 387-401. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035008352&partnerID=MN8TOARS.
    10.1016/S0145-305X(01)00002-7
  40. Dos Santos, N.M.S.; Taverne-Thiele, J.J.; Barnes, A.C.; Ellis, A.E.; Rombout, J.H.W.M.. "Kinetics of juvenile sea bass (Dicentrarchus labrax, L.) systemic and mucosal antibody secreting cell response to different antigens (Photobacterium damselae spp. piscicida, Vibrio anguillarum and DNP)". Fish and Shellfish Immunology 11 4 (2001): 317-331. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035343750&partnerID=MN8TOARS.
    10.1006/fsim.2000.0320
  41. Dos Santos, N.M.S.; Taverne-Thiele, J.J.; Barnes, A.C.; Van Muiswinkel, W.B.; Ellis, A.E.; Rombout, J.H.W.M.. "The gill is a major organ for antibody secreting cell production following direct immersion of sea bass (Dicentrarchus labrax, L.) in a Photobacterium damselae ssp. piscicida bacterin: An ontogenetic study". Fish and Shellfish Immunology 11 1 (2001): 65-74. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035234070&partnerID=MN8TOARS.
    10.1006/fsim.2000.0295
  42. Dos Santos, N.M.S.; Romano, N.; De Sousa, M.; Ellis, A.E.; Rombout, J.H.W.M.. "Ontogeny of B and T cells in sea bass (Dicentrarchus labrax, L.)". Fish and Shellfish Immunology 10 7 (2000): 583-596. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034288479&partnerID=MN8TOARS.
  43. López-Dóriga, M.V.; Barnes, A.C.; Dos Santos, N.M.S.; Ellis, A.E.. "Invasion of fish epithelial cells by Photobacterium damselae suksp. piscicida : Evidence for receptor specificity, and effect of capsule and serum". Microbiology 146 1 (2000): 21-30. http://www.scopus.com/inward/record.url?eid=2-s2.0-0041405584&partnerID=MN8TOARS.
  44. Dos Santos, N.M.S.; Taverne, N.; Taverne-Thiele, A.J.; De Sousa, M.; Rombout, J.H.W.M.. "Characterisation of monoclonal antibodies specific for sea bass (Dicentrarchus labrax L.) IgM indicates the existence of B cell subpopulations". Fish and Shellfish Immunology 7 3 (1997): 175-191. http://www.scopus.com/inward/record.url?eid=2-s2.0-0031116821&partnerID=MN8TOARS.
    10.1006/fsim.1996.0073
Preprint
  1. Lisboa, Johnny; Pereira, Cassilda; Pinto, Rute D.; Rodrigues, Inês S.; Pereira, Liliana M. G.; Pinheiro, Bruno; Oliveira, Pedro; et al. "Structural and functional characterization of the NF-¿B-targeting toxin AIP56 fromPhotobacterium damselaesubsp.piscicidareveals a novel mechanism for membrane interaction and translocation". 2023. http://dx.doi.org/10.1101/2023.05.04.539443.
    10.1101/2023.05.04.539443
Thesis / Dissertation
  1. dos Santos, Nuno M.S.. "Development of Immunity in Sea Bass: A Study Towards Vaccination Against Pseudotuberculosis". PhD, Wageningen University and Research Wageningen Institute of Animal Sciences, 2000. https://library.wur.nl/WebQuery/wurpubs/66133.

Intellectual property

Patent
  1. dos Santos, Nuno M.S.. 2014. "AIP56 derived peptides".
  2. dos Santos, Nuno M.S.. 2005. "Protein from Photobacterium damselae and use thereof".
  3. dos Santos, Nuno M.S.. 2004. "Inactivated Nodavirus Vaccine".
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2022/05 AIP56 and AIP56-like toxins: a family of bacterial toxins that target NF-kB 5th European NF-kappaB subunit workshop
(Porto, Portugal)
2021/09 Structural and functional characterization of NiTox: a new AB-toxin from Vibrio nigripulchritudo lethal to shrimp 20th EAFP
(Virtual)
2021/06 AIP56, a single chain AB toxin with a unique structural organization 7th International Iberian Biophysics Congress
(Coimbra, Portugal)
2019/06/24 Structural insights on AIP56 and AIP56-related toxins ETOX19, European Workshop on Bacterial Protein Toxins
(Davos, Switzerland)
2017/06/14 AIP56, an apoptosis inducing protein: basic research and applications CNC.IBILI Seminars
UC-Biotech (Cantanhede, Portugal)
2017/06 The type II secretion of Photobacterium damselae piscicida mediates extracellular release of the apoptogenic toxin AIP56 ETOX18, European Workshop on Bacterial Protein Toxins
Institute Pasteur (Paris, France)
2016/03/05 AB toxins: basic research and applications IV AEICBAS Biomedical Congress
Associação de Estudntes do Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto (Porto, Portugal)
2015/06 AIP56: a short-trip AB toxin with a peculiar translocation domain ETOX 17, 17th European Workshop on Bacterial Protein Toxins
Universidade do Porto Instituto de Biologia Molecular e Celular (Braga, Portugal)
2013/06 Interaction of AIP56 with cells ETOX 16, European Workshop on Bacterial Protein Toxins
(Freiburg, Germany)
2011/05 AIP56, a potential target as vaccine against Photobacterium damselae piscicida The Nodavirus challenge to aquaculture, 2011 Mediterranean fish health workshop
University of Malaga (Malaga, Spain)
2011/05 Programmed cell death in immune responses Fish Immunology Workshop
Wageningen University (Wageningen, Netherlands)
2009/09 Studies on AIP56, a key virulent factor of Photobacterium damselae subs. piscicida 4th EAFP (European Association of Fish Pathologists) International Conference
(Praga, Czech Republic)
2009/09 Caspase-1 and IL-1beta processing in sea bass (Dicentrarchus labrax) 14th EAFP (European Association of Fish Pathologists) International Conference
(Prague, Czech Republic)
2009/06 Structural and functional studies on AIP56, a novel bacterial apoptogenic toxin ETOX14, 14th European Workshop on Bacterial Protein Toxins
(Obernai, France)
2006/10 Molecular characterization, 3D modeling and expression analysis of sea bass (Dicentrarchus labrax L.) interleukine-10 XXXII Annual Meeting of the Sociedade Portuguesa de Imunologia, Innate and acquired immunity: evolution and regulation
(Braga, Portugal)
2006/07 Molecular cloning and expression analysis of sea bass (Dicentrarchus labrax L.) interleukin-12 p40 and p35 subunits 10th ISDCI (International Society of Developmental and Comparative Immunology) congress
(Charleston, United States)
2003/06/05 Mucosal immune responses after immersion vaccination of fish and the effect of hyperosmotic pre-treatment 9th ISDCI (International Society of Developmental and Comparative Immunology) Congress
(St Andrews, Scotland, United Kingdom)
2003/04 Protective immunity of proteins associated with invasion of fish epithelial cells by Photobacterium damselae ssp. piscicida 3rd International Symposium on Fish Vaccinology
(Bergen, Norway)

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2021/01/01 - Current Development of an antigen delivery platform based on AB toxins with specific tropism for APCs
Supervisor of Bruno Miguel da Silva Pinheiro
 Biologia Molecular e Celular (PhD)
Universidade do Porto, Portugal

Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal

Universidade do Porto Faculdade de Ciências, Portugal

Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2021/01/01 - Current Characterization of a novel family of AB toxins with potential to be used as antigen delivery vehicles
Supervisor of Inês Lua Freitas
 Biologia Molecular e Celular (PhD)
Universidade do Porto, Portugal

Universidade do Porto Faculdade de Ciências, Portugal

Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal

Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2018/07/15 - Current Johnny Marques Lisboa
Supervisor of Johnny Marques Lisboa
 Investigador Contratado (Other)
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal

Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2020 - 2021 Can AIP56, Apoptosis Inducing Protein of 56kDa, be used as an antigen delivery system for Leishmaniasis?
Supervisor of Amber Van Laer
 Master in Biomedical Sciences (Master)
Universiteit Antwerpen, Belgium
2020 - 2021 Biotechnological application of AIP56, an AB-toxin from Photobacterium damselae subsp. piscicida
Supervisor of Ana Catarina Pinto Mendes de Moura
 Master in Biochemistry (Master)
Universidade do Porto, Portugal
2020 - 2021 The understanding of AIP56-like proteins
Supervisor
 Magistère Européen de Génétique (Master)
Université de Paris, France
2015/02/01 - 2020 Mediators of inflammation: unravelling the role of methionine and tryptophan during infection
Co-supervisor of Marina Machado Casimiro
Ciência Animal (PhD)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2019 - 2019 Development of an antigen delivery platform based on the AIP56 toxin
Supervisor of Bruno Miguel da Silva Pinheiro
 Biologia Celular e Molecular (Master)
Universidade do Porto Faculdade de Ciências, Portugal

Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal

Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2015/03/01 - 2018/12/31 Ana Maria Silva do Vale
Supervisor of Ana Maria Silva do Vale
 Pos-doc (Other)
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal

Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2016/05/01 - 2018/07/14 Johnny Marques Lisboa
Supervisor of Johnny Marques Lisboa
 Pos-doc (Other)
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal

Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2018 - 2018 AIP56, an AB toxin from the fish pathogenic bacterium Photobacterium damselae subsp. piscicida, as a possible drug and/or antigen delivery system for leishmaniasis
Supervisor of Laura Dirkx
 Biomedical Sciences (Master)
Universiteit Antwerpen Faculteit Farmaceutische Biomedische en Diergeneeskundige Wetenschappen, Belgium

Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal

Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2017 - 2017 Zebrafish as model for studying AIP56 toxicity
Supervisor of Maria Beatriz de Sousa Figueiredo
 Université de Nantes UFR Sciences et Techniques (Master)
Université de Nantes UFR Sciences et Techniques, France

Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal

Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2015/10/01 - 2016/04/30 Johnny Marques Lisboa
Supervisor of Johnny Marques Lisboa
 Pos-doc (Other)
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal

Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2014/05/01 - 2015/05/14 Johnny Marques Lisboa
Supervisor of Johnny Marques Lisboa
 Pos-doc (Other)
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal

Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2009/01/01 - 2015/02/23 Interaction of the apoptogenic AB toxin AIP56 with cells and its intracellular trafficking
Supervisor of Liliana Marisa Gonçalves Pereira
 Ciências Biomédicas (PhD)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal

Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2014 - 2014 Biotechnological and clinical potential of AIP56 toxin
Co-supervisor of Inês Salvador Rodrigues
 Bioquímica (Master)
Universidade do Porto Faculdade de Ciências, Portugal

Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2008/01/01 - 2013/10/25 MHC in sea bass: molecular and structural insights to class I antigen presentation
Supervisor of Rute Daniela Pinheiro da Silva Pinto
 Ciências Biomédicas (PhD)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal

Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2008/01/01 - 2013/07/16 Structural and functional characterization of AIP56, an apoptogenic exotoxin of Photobacterium damselae piscicida
Supervisor of Daniela Sá Pereira e Silva
 Ciências Biomédicas (PhD)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal

Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2008/01/01 - 2012/11/30 Role played by caspase-1, -3, -8 and -9 in sea bass phagocyte apoptosis induced by an exotoxin of Photobacterium damselae piscicida
Supervisor of Marta Inês Rocha Reis
Ciências Biomédicas (PhD)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal

Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2005 - 2011 Carolina Costa-Ramos
Supervisor of Carolina Costa-Ramos
 Pos-doc (Other)
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2010 - 2010 Molecular cloning and characterization of sea bass ERp57, calnexin and calreticulin genes
Supervisor of Ana Raquel Pinto Moreira
 Bioquímica (Master)
Universidade do Porto Faculdade de Ciências, Portugal

Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2010 - 2010 Sea bass macrophage colony stimulating factor receptors
Supervisor of Márcio Adriano Guiomar de Oliveira
 Biologia Molecular e Celular (Master)
Universidade de Aveiro Departamento de Biologia, Portugal

Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2003/09/01 - 2008/04/30 Study of Sea Bass Cytokines: Molecular Characterization and Expression Analysis in Response to Photobacterium damselae ssp. piscicida Exposure
Supervisor of Diana Esperança dos Santos Nascimento
 Ciências Biológicas (PhD)
University of Aberdeen, United Kingdom

Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2003 - 2003 Estudo da composição mineral e da acumulação de metais em pregado (Scophthalmus maximus) criado em sistema de recirculação
Supervisor of Elisabete Tavares
Universidade de Aveiro Departamento de Biologia, Portugal

Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal

Event organisation

Event name
Type of event (Role) 		Institution / Organization
2015/06/20 - 2015/06/27 ETOX17, 17th European Workshop on Bacterial Protein Toxins (2015/06/20 - 2015/06/27)
Workshop (President of the Organising Committee)
 Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal

Jury of academic degree

Topic
Role 		Candidate name (Type of degree)
Institution / Organization
2013 Structural and functional characterization of AIP56, an apoptogenic exotoxin of Photobacterium damselae piscicida
Supervisor
 Daniela Sá Pereira e silva (PhD)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2013 MHC in sea bass: molecular and structural insights to class I antigen presentation
Supervisor
 Rute Daniela Pinheiro da Silva Pinto (PhD)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2012 Molecular and genetic characterization of sea bass caspases
Supervisor
 Marta Inês Rocha reis (PhD)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2011 Sistema inmune inespecífico en dorada (Sparus aurata): activación del sistema interferón
(Thesis) Main arguer
 Jimena Bravo Garcia (PhD)
Universidad de Las Palmas de Gran Canaria Instituto Universitario de Sanidad Animal y Seguridad Alimentaria, Spain
2011 Iron and Immunity: the Role of Iron During Bacterial Infection in Sea Bass (Dicentrarchus Labarx)
(Thesis) Arguer
 João Filipe Pereira Villares Neves (PhD)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2010 Apoptosis: How It Restricts Legionella Pneumophila Intracellular Replication and How It Can Be Neutralized by a Type IV Effector Protein
(Thesis) Main arguer
 Catarina Sofia Viana Nogueira (PhD)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal

Ad Hoc journal article review

Journal title (ISSN) Publisher
2018/09/03 - Current PLoS Pathogens Public Library of Science
2016/01/05 - Current Diseases of Aquatic Organisms Inter-Research Science Publisher
2015/07/14 - Current Cellular Microbiology (1462-5822) Wiley (Blackwell Publishing)
2014/11/18 - Current Gene (0378-1119) Elsevier
2014/01/10 - Current Infection and Immunity (1098-5522) American Society for Microbiology
2013/09/22 - Current PLoS ONE (1932-6203) Public Library of Science
2013/03/26 - Current FEBS Journal (1742-4658) Wiley (Blackwell Publishing)
2012/01/03 - Current Veterinary Microbiology (0378-1135) Elsevier
2011/07/04 - Current Research in Microbiology (0923-2508) Elsevier
2011/02/11 - Current Chemistry and Ecology (1029-0370) Informa UK (Taylor & Francis)
2009/06/05 - Current Developmental & Comparative Immunology (0145-305X) Elsevier
2009 - Current APOPTOSIS (1573-675X) Springer-Verlag
2005 - Current Fish & Shellfish Immunology (1095-9947) Elsevier

Committee member

Activity description
Role 		Institution / Organization
2014 - 2022 Friday Noon Seminars: series of seminars that take place at i3S (previously at IBMC) on Fridays at noon, with internationally renowned guest speakers
Coordinator
 Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2008 - 2018 Animal Ethics Commission
Member
 Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal

Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal

Conference scientific committee

Conference name Conference host
2023/06/25 - 2023/06/29 ETOX21, European Workshop on Bacterial Protein Toxins
2021/06/28 - 2021/07/01 ETOX20, European Workshop on Bacterial Protein Toxins
2019/06/22 - 2019/06/26 ETOX19, European Workshop on Bacterial Protein Toxins
2017/06/26 - 2017/06/30 ETOX18, European Workshop on Bacterial Protein Toxins

Course / Discipline taught

Academic session Degree Subject (Type) Institution / Organization
2010 - 2019 Bacterial AB Toxins Biologia Básica e Aplicada (Doutoramento) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal

Universidade do Porto Faculdade de Ciências, Portugal

Universidade do Porto Faculdade de Medicina, Portugal
2008 - 2019 Bacterial AB toxins Bioquímica (Mestrado integrado) Universidade do Porto Faculdade de Ciências, Portugal
2018 - 2018 Bacterial AB toxins Biociências Moleculares (Doutoramento) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2018 - 2018 Bacterial AB toxins PhD Program Biology at the Host Microbe Interface (Doutoramento) Instituto Gulbenkian de Ciência, Portugal

Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2016 - 2016 Bacterial AB toxins Biociências Moleculares (Doutoramento) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Journal scientific committee

Journal title (ISSN) Publisher
2021/08/26 - Current PLoS Pathogens (1553-7374) Public Library of Science