María Lourdes Renart Pérez (9414-B2B0-87F7)

Curriculum Vitae

María Lourdes Renart Pérez

María Lourdes Renart Pérez is a researcher at the recently created Instituto de Innovación, Desarrollo e Innovación en Biotecnología Sanitaria de Elche (IDiBE), part of the Universidad Miguel Hernández (UMH, Elche, Spain). She completed her doctorate in 2010 at the Insituto de Biología Molecular y Celular from the same university. S he studied Biochemistry at the Universidad de Buenos Aires (Argentina) and worked as a research trainee on the free radical and antioxidants field with the team of Prof. César Fraga. In addition to her Ph.D. degree, she is also has a degree (Licenciatura) In Biochemistry (2007) and Pharmacy (2013) from the UMH. In 2003, she was awarded with an "FPI" predoctoral fellowship from Ministerio de Educación y Ciencia of Spain and started officially her Ph.D. at the laboratory of Prof. José Manuel González Ros. The aim of her thesis was the study of the structure-function link in ion channels, using the prokaryotic potassium channel KcsA as a model. The dissertation got a "Sobresaliente cum laude" grade. This work, among all the investigation lines in the laboratory, were presented several times in national and international conferences and published in prestigious international scientific journals. In 2014, she started a post-doctoral training among the advanced fluorescence field at the laboratory of Prof. Manuel Prieto in Lisbon (IST, Universidade de Lisboa). That work is now part of a thesis project, which she is co-directing with Profs. Manuel Prieto and Ana Coutinho. So far, her laboratory skills include: heterelogous expression of membrane proteins in E.coli systems, membrane proteins purification by several types of chromatography techniques, SDS-PAGE, BN-PAGE and agarose electrophoresis, site-directed mutagenesis by PCR, plasmidic DNA amplification and purification, notions of electrophysiological recordings (patch-clamp), circular dichroism and steady-state fluorescence spectroscopy, membrane proteins folding analysis by chemical and physical denaturation, protein reconstitution into liposomes and labelling with fluorescent dyes, fluorescence microscopy, time-resolved fluorescence and anisotropy spectroscopy, among others. To date her general quality indicators are: Total publications: 26; H-index: 9; sum of times cited: 497; citing articles: 418. She works in the area(s) of Natural sciences, Biological Sciences with emphasis on Biophysics.

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Identification

Personal identification

Full name: María Lourdes Renart Pérez

Citation names

Renart, María Lourdes
Lourdes Renart, M
Renart, Lourdes
Renart, ML

Author identifiers

Ciência ID: 9414-B2B0-87F7
ORCID iD: 0000-0002-6717-4756

Email addresses

lrenart@umh.es (Professional)

Telephones

Telephone

(+34) 966658942 (Professional)

Addresses

Avda de la Universidad s/n Edificio Torregaitán L2.10, 03202, Elche, Alicante, Spain (Professional)

Knowledge fields

Natural sciences - Biological Sciences - Biophysics

Languages

Language	Speaking	Reading	Writing	Listening	Peer-review
Spanish; Castilian (Mother tongue)
English	Advanced (C1)	Advanced (C1)	Advanced (C1)	Advanced (C1)	Advanced (C1)
Portuguese	Beginner (A1)	Beginner (A1)	Beginner (A1)	Beginner (A1)	Beginner (A1)

Education

	Degree	Classification
2013 Concluded	Licenciatura en Farmacia (Licenciatura) Universidad Miguel Hernández de Elche Facultad de Farmacia, Spain
2010 Concluded	Biología Molecular y Celular (Doutoramento) Universidad Miguel Hernández de Elche, Spain " Iones y fosfolípidos como efectores en la estructura y estabilidad del canal de potasio KcsA" (THESIS/DISSERTATION)	Sobresaliente cum laude
2006 - 2007 Concluded	Licenciatura en Bioquímica (Licenciatura) Universidad Miguel Hernández de Elche, Spain	8.08/10
1996 - 2001 Concluded	Bioquímica (Licenciatura) Universidad de Buenos Aires, Argentina	8.25/10

Affiliation

Science

	Category Host institution	Employer
2019/12 - Current	Contracted Researcher (Research)	Universidad Miguel Hernández de Elche - Instituto de Investigación, Desarrollo e Innovación en Biotecnología Sanitaria de Elche, Spain
	Universidad Miguel Hernández de Elche - Instituto de Investigación, Desarrollo e Innovación en Biotecnología Sanitaria de Elche, Spain
2014/09 - 2016/01	Postdoc (Research)	Fundação para a Ciência e a Tecnologia, Portugal
	Universidade de Lisboa Centro de Química-Física Molecular, Portugal
2007 - 2014	Contracted Researcher (Research)	Universidad Miguel Hernández de Elche, Spain
	Universidad Miguel Hernández de Elche Instituto de Biología Molecular y Celular, Spain
1999 - 2001	Research Trainee (Research)	Universidad de Buenos Aires, Argentina
	Universidad de Buenos Aires Facultad de Farmacia y Bioquimica, Argentina

Projects

Grant

	Designation	Funders
2019/12 - Current	Estudio de los estados conformacionales del filtro de selectividad de los canales iónicos: hacia la comprensión de la permeación, selectividad e inactivación (AEI)/PGC2018-093505-B-I00 Researcher Universidad Miguel Hernández de Elche - Instituto de Investigación, Desarrollo e Innovación en Biotecnología Sanitaria de Elche, Spain	Ongoing
2017/12 - 2019/03	Bases Moleculares de la modulación de canales iónicos BFU2015- 66612-P Researcher Universidad Miguel Hernández de Elche Instituto de Biología Molecular y Celular, Spain	Concluded
2014/09 - 2016/01	Nanomateriais Multifuncionais para Microscopia de Fluorescência e para Sensores RECI/CTM-POL/0342/2012 Post-doc Fellow Universidade de Lisboa Centro de Química-Física Molecular, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2011/05 - 2014/03	The Spanish ion channel initiative (SICI) CSD2-2008-00005 Researcher Universidad Miguel Hernández de Elche Instituto de Biología Molecular y Celular, Spain	Gobierno de España Ministerio de Ciencia e Innovación Concluded
2009/05 - 2011/11	Estudio de interacciones funcionalmente relevantes en proteínas de membrana: allanando el camino a nuevas aproximaciones al descubrimiento de fármacos BFU2009-08346 Researcher Universidad Miguel Hernández de Elche Instituto de Biología Molecular y Celular, Spain	Gobierno de España Ministerio de Ciencia e Innovación Concluded
2007/06 - 2008/12	Canales iónicos: modelos para el estudio de interacciones moleculares en proteínas de membrana y para la identificación de nuevas dianas de potencial actuación farmacológica BFU2005-00749/BMC Researcher Universidad Miguel Hernández de Elche Instituto de Biología Molecular y Celular, Spain	Concluded
2003/05 - 2007/04	Interacciones Moleculares en Neurobiología: conocimientos básicos y posibles aplicaciones BFI2002-03410 PhD Student Fellow Universidad Miguel Hernández de Elche Instituto de Biología Molecular y Celular, Spain	Gobierno de España Ministerio de Educación y Ciencia Concluded

Outputs

Publications

Conference abstract	Renart, María Lourdes; Díaz-García, Clara; Giudici, Ana Marcela; Poveda, José Antonio; González-Ros, José M.; Berberan-Santos, Mário; Coutinho, Ana; Prieto, Manuel. "Conformational Plasticity of the KcsA Channel from Advanced Time-Resolved Homo-FRET Methodologies". Paper presented in Biophysical Society 64th Annual Meeting, San Diego, 2020. Published Renart, María Lourdes; Giudici, Ana Marcela; Poveda JA; Fedorov, Aleksander; Berberan-Santos, Mário; Prieto, Manuel; González-Ros, José M.; Coutinho, Ana. "Probing conformational changes of K plus channel KcsA by time-resolved homo-FRET studies". Paper presented in 19th IUPAB Congress / 11th EBSA Congress, Edinburgh, 2017. Published Renart, María Lourdes; Giudici, Ana Marcela; Montoya, Estefanía; Poveda, José Antonio; Fedorov, Aleksander; Berberan-Santos, Mário; Prieto, Manuel; González-Ros, José M.; Coutinho, Ana. "Homo-FRET studies of K plus channel KcsA: a closer look to selectivity filter dynamics and regulation". Paper presented in 10th European-Biophysical-Societies-Association (EBSA) European Biophysics Congress, Dresden, 2015. Published
Journal article	Renart, María Lourdes; Giudici, Ana Marcela; Díaz-García, Clara; Molina, María Luisa; Morales, Andrés; González-Ros, José M.; Poveda, José Antonio. "Modulation of Function, Structure and Clustering of K+ Channels by Lipids: Lessons Learnt from KcsA". International Journal of Molecular Sciences 21 7 (2020): 2554. http://dx.doi.org/10.3390/ijms21072554. Open access • Published • 10.3390/ijms21072554 M. Lourdes Renart; A. Marcela Giudici; José A. Poveda; Aleksander Fedorov; Mário N. Berberan-Santos; Manuel Prieto; Clara Díaz-García; José M. González-Ros; Ana Coutinho. "Conformational plasticity in the KcsA potassium channel pore helix revealed by homo-FRET studies". Scientific Reports 9 1 (2019): https://doi.org/10.1038/s41598-019-42405-5. 10.1038/s41598-019-42405-5 Ana Marcela Giudici; María Lourdes Renart; Clara Díaz-García; Andrés Morales; jose antonio poveda larrosa; José Manuel González-Ros. "Accessibility of Cations to the Selectivity Filter of KcsA in the Inactivated State: An Equilibrium Binding Study". International Journal of Molecular Sciences (2019): http://www.mdpi.com/1422-0067/20/3/689. 10.3390/ijms20030689 Montoya E; Renart, María Lourdes; Marcela Giudici A; Poveda JA; Fernández AM; Morales A; González-Ros JM. "Differential binding of monovalent cations to KcsA: Deciphering the mechanisms of potassium channel selectivity.". Biochimica et Biophysica Acta (BBA) - Biomembranes 1859 5 (2017): 779-788. http://europepmc.org/abstract/med/28088447. Published • 10.1016/j.bbamem.2017.01.014 Poveda JA; Marcela Giudici A; Lourdes Renart M; Morales A; González-Ros JM. "Towards understanding the molecular basis of ion channel modulation by lipids: Mechanistic models and current paradigms.". Biochimica et Biophysica Acta (BBA) - Biomembranes 1859 9(B) (2017): 1507-1516. http://europepmc.org/abstract/med/28408206. Published • 10.1016/j.bbamem.2017.04.003 Renart, María Lourdes; Montoya, Estefanía; Giudici, Ana Marcela; Poveda, José Antonio; Fernández, Asia; Morales, Andrés; González-Ros, José M.. "Selective exclusion and selective binding both contribute to ion selectivity in KcsA, a model potassium channel". The Journal of biological chemistry 292 37 (2017): 15552-15560. https://doi.org/10.1074/jbc.M117.795807. Published • 10.1074/jbc.m117.795807 Molina ML; Giudici AM; Poveda JA; Fernández-Ballester G; Montoya E; Renart ML; Fernández AM; et al. "Competing Lipid-Protein and Protein-Protein Interactions Determine Clustering and Gating Patterns in the Potassium Channel from Streptomyces lividans (KcsA).". Journal of Biological Chemistry 290 42 (2015): 25745-25755. http://europepmc.org/abstract/med/26336105. Published • 10.1074/jbc.m115.669598 Poveda JA; Giudici AM; Renart ML; Molina ML; Montoya E; Fernández-Carvajal A; Fernández-Ballester G; Encinar JA; González-Ros JM. "Lipid modulation of ion channels through specific binding sites.". Biochimica et Biophysica Acta (BBA) - Biomembranes 1838 6 (2014): 1560-1567. http://europepmc.org/abstract/med/24211605. Published • 10.1016/j.bbamem.2013.10.023 Giudici AM; Molina ML; Ayala JL; Montoya E; Renart ML; Fernández AM; Encinar JA; et al. "Detergent-labile, supramolecular assemblies of KcsA: relative abundance and interactions involved.". Biochimica et Biophysica Acta (BBA) - Biomembranes 1828 2 (2013): 193-200. http://europepmc.org/abstract/med/23022492. Published • 10.1016/j.bbamem.2012.09.020 Calcutta A; Jessen CM; Behrens MA; Oliveira CL; Renart ML; González-Ros JM; Otzen DE; et al. "Mapping of unfolding states of integral helical membrane proteins by GPS-NMR and scattering techniques: TFE-induced unfolding of KcsA in DDM surfactant.". Biochimica et Biophysica Acta (BBA) - Biomembranes 1818 9 (2012): 2290-2301. http://europepmc.org/abstract/med/22525601. Published • 10.1016/j.bbamem.2012.04.005 Renart ML; Montoya E; Fernández AM; Molina ML; Poveda JA; Encinar JA; Ayala JL; et al. "Contribution of ion binding affinity to ion selectivity and permeation in KcsA, a model potassium channel.". Biochemistry 51 18 (2012): 3891-3900. http://europepmc.org/abstract/med/22509943. 10.1021/bi201497n Renart ML; Triano I; Poveda JA; Encinar JA; Fernández AM; Ferrer-Montiel AV; Gómez J; González Ros JM. "Ion binding to KcsA: implications in ion selectivity and channel gating.". Biochemistry 49 44 (2010): 9480-9487. http://europepmc.org/abstract/med/20925387. Published • 10.1021/bi101235v Triano I; Barrera FN; Renart ML; Molina ML; Fernández-Ballester G; Poveda JA; Fernández AM; et al. "Occupancy of nonannular lipid binding sites on KcsA greatly increases the stability of the tetrameric protein.". Biochemistry 49 25 (2010): 5397-5404. http://europepmc.org/abstract/med/20481584. Published • 10.1021/bi1003712 Neira JL; Román-Trufero M; Contreras LM; Prieto J; Singh G; Barrera FN; Renart ML; Vidal M. "The transcriptional repressor RYBP is a natively unfolded protein which folds upon binding to DNA.". Biochemistry 48 6 (2009): 1348-1360. http://europepmc.org/abstract/med/19170609. Published • 10.1021/bi801933c Molina ML; Barrera FN; Encinar JA; Renart ML; Fernández AM; Poveda JA; Santoro J; et al. "N-type inactivation of the potassium channel KcsA by the Shaker B "ball" peptide: mapping the inactivating peptide-binding epitope.". Journal of Biological Chemistry 283 26 (2008): 18076-18085. http://europepmc.org/abstract/med/18430729. Published • 10.1074/jbc.m710132200 Barrera FN; Renart ML; Poveda JA; de Kruijff B; Killian JA; González-Ros JM. "Protein self-assembly and lipid binding in the folding of the potassium channel KcsA.". Biochemistry 47 7 (2008): 2123-2133. http://europepmc.org/abstract/med/18205389. Published • 10.1021/bi700778c Renart ML; Barrera FN; Molina ML; Encinar JA; Poveda JA; Fernández AM; Gómez J; González-Ros JM. "Effects of conducting and blocking ions on the structure and stability of the potassium channel KcsA.". Journal of Biological Chemistry 281 40 (2006): 29905-29915. http://europepmc.org/abstract/med/16815844. Published • 10.1074/jbc.m602636200 Molina ML; Barrera FN; Fernández AM; Poveda JA; Renart ML; Encinar JA; Riquelme G; González-Ros JM. "Clustering and coupled gating modulate the activity in KcsA, a potassium channel model.". Journal of Biological Chemistry 281 27 (2006): 18837-18848. http://europepmc.org/abstract/med/16670090. Published • 10.1074/jbc.m600342200 Encinar JA; Molina ML; Poveda JA; Barrera FN; Renart ML; Fernández AM; González-Ros JM. "The influence of a membrane environment on the structure and stability of a prokaryotic potassium channel, KcsA.". FEBS Letters 579 23 (2005): 5199-5204. http://europepmc.org/abstract/med/16150445. Published • 10.1016/j.febslet.2005.08.038 Barrera FN; Renart, María Lourdes; Molina ML; Poveda JA; Encinar JA; Fernández AM; González-Ros JM. "Unfolding and refolding in vitro of a tetrameric, alpha-helical membrane protein: the prokaryotic potassium channel KcsA". Biochemistry 44 43 (2005): 14344-14352. https://doi.org/10.1021/bi050845t. 10.1021/bi050845t Lotito SB; Renart ML; Fraga CG. "Assessing the antioxidant capacity in the hydrophilic and lipophilic domains: study of a sample of Argentine wines.". Annals of the New York Academy of Sciences 957 (2002): 284-287. http://europepmc.org/abstract/med/12074982. Published • 10.1111/j.1749-6632.2002.tb02926.x Lotito SB; Actis-Goretta L; Renart ML; Caligiuri M; Rein D; Schmitz HH; Steinberg FM; Keen CL; Fraga CG. "Influence of oligomer chain length on the antioxidant activity of procyanidins.". Biochemical and Biophysical Research Communications 276 3 (2000): 945-951. http://europepmc.org/abstract/med/11027573. Published • 10.1006/bbrc.2000.3571
Thesis / Dissertation	Renart, María Lourdes. "Iones y fosfolípidos como efectoresen la estructura y estabilidad del canal de potasio KcsA". PhD, Universidad Miguel Hernández de Elche Instituto de Biología Molecular y Celular, 2010.

Activities

Oral presentation

	Presentation title	Event name Host (Event location)
2020/02	Conformational Plasticity of the KcsA Channel from Advanced Time-Resolved Homo-FRET Methodologies	64th Annual Meeting of the Biophysical-Society Biophysical Society (San Diego, United States)
2018/09/11	The membrane potassium channel KcsA - Exact analytical solutions for advanced homo-FRET to retrieve structural information	VIth Jornadas Ibéricas de Fotoquímica Grupo de Fotoquímica of the Sociedade Portuguesa de Química (SPQ) and the Grupo Especializado de Fotoquimica (GRUFO) of the Real Sociedad Española de Química (RSEQ) (Aveiro, Portugal)
2018/06/20	Exploring potassium channels selectivity and inactivation through steady-state and time-resolved fluorescence spectroscopy	6th International Iberian Biophysics Congress X Iberoamerican Congress of Biophysics Sociedad Española de Biofísica, Portuguese Biophysical Society, Latin American Federation of Biophysical Societies (Castellón de la Plana, Spain)
2014/11/06	The potassium channel KcsA: A model for developing useful tools in the understanding of potassium channels and design new potential drugs	XVI Congreso de Sociedad de Biofísica de España Sociedad Española de Biofísica (Madrid, Spain)
2011/07/11	Protein-protein and lipid-protein interactions in KcsA	International Workshop on Integrative Research on Ion Channels SICI (The Spanish Ion Channel Iniciative) (Elche, Spain)
2011/06/01	Electroforesis “blue-native” para la cuantificación de la auto-asociación de KcsA	XI Congreso de Sociedad de Biofísica de España Sociedad Española de Biofísica (Murcia, Spain)
2011/02/02	Specific ions, specific lipids: sources of protein stability in an ion channel model	RECI 3: Trends and challenges in ion channel research RECI (Red Española de Canales Iónicos). (Tenerife, Spain)
2009/10/15	On the molecular basis of ion channel selectivity and conduction	II Reunión Española de Canales Iónicos (RECI) RECI (Red Española de Canales Iónicos). (Valladolid, Spain)
2007/07/14	Unfolding and refolding of a predominantly ?-helical, tetrameric potassium channel protein	6 th EBSA European Biophysics Congress EBSA (London, United Kingdom)
2007/05/17	El canal de potasio KcsA: un modelo para muchos usos	I RECI: Reunión Española de Canales Iónicos RECI (Red Española de Canales Iónicos). (Alicante, Spain)
2007/03/08	El canal de potasio KcsA: modelo para el estudio de la oligomerización y el plegamiento de proteínas de membrana	II Reunión del grupo de plegamiento y estructura de proteínas Grupo de plegamiento y estructura de proteínas de la SEBBM (Alicante, Spain)

Supervision

	Thesis Title Role	Degree Subject (Type) Institution / Organization
2017 - Current	Structural insights into the molecular basis of lipid-dependent gating of KcsA, a model potassium channel Co-supervisor	Universidade de Lisboa Instituto Superior Técnico, Portugal

Event participation

	Activity description Type of event	Event name Institution / Organization
2013 - 2013	Invited speaker in the series of conferences "En las fronteras del conocimiento" Convocatoria de Innovación y Mejora Docente para la divulgación del conocimiento en Doctorados UMH Conference	Iones y fosfolípidos como efectores en la estructura y estabilidad del canal de potasio KcsA Universidad Miguel Hernández de Elche, Spain

Association member

	Society Organization name	Role
2009 - Current	Sociedad de Biofísica de España	Adherent member
2003 - Current	Sociedad Española de Bioquímica y Biología Molecular	Adherent member

Course / Discipline taught

	Academic session	Degree Subject (Type)	Institution / Organization
2017 - Current	Rutas de aprendizaje para el personal de administración y servicios en formato de nanocursos (www.nanocursos.com)	NanoCursos de Conceptos y Técnicas de Uso Habitual en el Estudio de Biomoléculas	Universidad Miguel Hernández de Elche, Spain
1999 - 2001	Biochemistry	Fisicoquímica (Licence)	Universidad de Buenos Aires Facultad de Farmacia y Bioquimica, Argentina

Mentoring / Tutoring

	Topic	Student name
2019 - 2019	Estudio de la conformación del estado activado de KcsA mediante bloqueadores de alquilamonio (Final year project)	Francisco Javier Vázquez Gallardo
2016 - 2016	Estudio del mecanismo de selectividad en canales de potasio (Final year project)	Eduardo Puerta Baile

Other jury / evaluation

	Activity description	Institution / Organization
2020/02 - Current	Reviewer for "Archives of Biochemistry and Biophysics" Journal

Distinctions

Other distinction

2012	Premio Extraordinario de Doctorado dentro del programa de Biología Molecular y Celular (2010-2011)
2007	Premio Extraordinario de Licenciatura en Bioquímica