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Identification

Personal identification

Full name
Tânia Filipa Arsénio Geraldes Francisco

Author identifiers

Ciência ID
941A-E434-265B
ORCID iD
0000-0003-3571-5164
Education
Degree Classification
2020/10/12 - 2020/10/16
Concluded
Introduction to Python and Machine Learning for the Health/ Life Sciences Course (Outros)
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2013
Concluded
Doutoramento em Ciências Biomédicas (Doutoramento)
Major in Doutoramento em Ciências Biomédicas
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
"The PEX5-mediated import pathway: Characterization of the translocation step" (THESIS/DISSERTATION)
Aprovada com Distinção
2012
Concluded
Course in Laboratory Animal Science (FELASA B) (Diploma de especialização)
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2006
Concluded
Biologia Celular e Molecular (pré-Bolonha) (Licenciatura)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
"Detecção Laboratorial de Agentes Infecciosos Susceptíveis de Causar Doença Neonatal" (THESIS/DISSERTATION)
15
Affiliation

Science

Category
Host institution
Employer
2019/01/01 - Current Contracted Researcher (Research) Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2014/01 - 2018 Postdoc (Research) Fundação para a Ciência e a Tecnologia, Portugal
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2009/01 - 2013/12 Researcher (Research) Fundação para a Ciência e a Tecnologia, Portugal
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2008/04 - 2008/12 Researcher (Research) Fundação para a Ciência e a Tecnologia, Portugal
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2007/06 - 2008/01 Researcher (Research) Universidade do Porto Faculdade de Medicina, Portugal
Universidade do Porto Faculdade de Medicina, Portugal
2006/10 - 2007/05 Research Trainee (Research) Universidade do Porto Faculdade de Medicina, Portugal
Universidade do Porto Faculdade de Medicina, Portugal

Teaching in Higher Education

Category
Host institution
Employer
2017/09 - Current Invited Assistant Professor (University Teacher) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
Projects

Grant

Designation Funders
2023/02/01 - 2025/01/31 A new component of the mammalian peroxisomal protein import machinery
2022.08378.PTDC
Researcher
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2023/12/01 - 2025 Unveiling peroxisomal homeostasis mechanisms – the role of LonP2
2023.02428.RESTART
Principal investigator
Fundação para a Ciência e a Tecnologia
Concluded
2021/01/15 - 2024/01/14 Investigating the molecular mechanisms of mammalian pexophagy
EXPL/BIA-MOL/1664/2021
Researcher
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2014/01/01 - 2018/12/31 The molecular mechanism of protein import into peroxisomes
Post-doc Fellow
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal

Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
Fundação para a Ciência e a Tecnologia
2009/01/01 - 2013/12/15 The PEX5-mediated import pathway: Characterization of the translocation step
SFRH/BD/61539/2009
PhD Student Fellow
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2008/01/01 - 2009/12/31 The molecular mechanisms of protein import into mammalian peroxisomes: a Pex5p- and Pex19p-centered perspective
PTDC/BIA-BCM/64771/2006
Research Fellow
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2007/01/01 - 2008/01/31 Genomic Imprinting in human spermatogenesis
Research Fellow
Universidade do Porto Faculdade de Medicina, Portugal
Fundação para a Ciência e a Tecnologia
Concluded

Contract

Designation Funders
2019/01/01 - Current Peroxisomal function and dynamics: players and mechanisms involved
DL 57/2016/CP1355/CT0019
Researcher
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
Ongoing
2022/04/18 - 2023/07/01 Investigating the molecular mechanisms of mammalian pexophagy
EXPL/BIA-MOL/1664/2021
Co-Principal Investigator (Co-PI)
Fundação para a Ciência e a Tecnologia
Ongoing

Other

Designation Funders
2016/05 - 2019/04 The Neurosciences and Neurologic Disease Research Initiative at i3S
NORTE-01-0145-FEDER-000008
Researcher
European Regional Development Fund
Concluded
2013 - 2015 Funding of RD Units Strategic Plan - 2013/2015 – OE
UID/BIM/04293/2013
Researcher
Fundação para a Ciência e a Tecnologia
Concluded
2013 - 2014 Projecto Estratégico - LA 2 - 2013-2014
PEst-C/SAU/LA0002/2013
Researcher
Fundação para a Ciência e a Tecnologia
2011 - 2012 Strategic Project - LA 2 - 2011-2012
PEst-C/SAU/LA0002/2011
Researcher
Fundação para a Ciência e a Tecnologia
Concluded
Outputs

Publications

Book chapter
  1. Ferreira, Maria J.; Rodrigues, Tony A.; Pedrosa, Ana G.; Francisco, Tânia; Azevedo, Jorge E.. "A Cell-Free In Vitro Import System for Peroxisomal Proteins Containing a Type 2 Targeting Signal (PTS2)". In Methods in Molecular Biology, 333-343. Springer US, 2023.
    10.1007/978-1-0716-3048-8_23
  2. Francisco, T.; Dias, A.F.; Pedrosa, A.G.; Grou, C.P.; Rodrigues, T.A.; Azevedo, J.E.. "Determining the topology of peroxisomal proteins using protease protection assays". 2017.
    Published • 10.1007/978-1-4939-6937-1_3
  3. Rodrigues, T.A.; Francisco, T.; Carvalho, A.F.; Pinto, M.P.; Grou, C.P.; Azevedo, J.E.. "Factors involved in ubiquitination and deubiquitination of PEX5, the peroxisomal shuttling receptor". 2014.
    10.1007/978-3-7091-1788-0_16
Conference abstract
  1. Freitas, MO; Francisco, Tânia; Rodrigues Tony; Lismont, C.; Domingues, P.; Pinto, MP.; Grou, CP; Fransen, M.; Azevedo JE. "LB-148 - Monomeric proteins are the preferential substrates of the peroxisomal protein import machinery". Paper presented in 40th FEBS Congress, the Biochemical Basis of Life (doi.org/10.1111/febs.13339), Berlin, 2015.
    Published
  2. Francisco, Tânia; Marques, Joana C.; Sousa, Sónia; Carvalho, Filipa; Fernandes, Susana; Silva, Júlia; Barros, Alberto; Sousa, Mário. "Defective Genomic Imprinting in Azoospermia". Paper presented in 24th European Society of Human Reproduction & Embriology - published in Human Reproduction, 2008.
    Published
Conference poster
  1. Ana Rita Silva; Francisco, Tânia; Rodrigues, Tony A; Fransen, Marc; Azevedo, Jorge E.. "Functional characterization of mammalian LONP2, a peroxisomal AAA+ protease". Paper presented in XXII NATIONAL CONGRESS OF BIOCHEMISTRY, 2024.
  2. Ferreira, MJ; Rodrigues, Tony A; Pedrosa, Ana G.; Gales, Luís; Salvador, Armindo; Francisco, Tânia; Azevedo, JE. "Glutathione to the rescue: the importance of glutathione in mammalian peroxisomal redox homeostasis". Paper presented in XXII NATIONAL CONGRESS OF BIOCHEMISTRY, 2024.
  3. Ana Rita Silva; Francisco, Tânia; Rodrigues, Tony A; Fransen, Marc; Azevedo, Jorge E.. "Functional characterization of mammalian Lonp2, a peroxisomal AAA+ protease". Paper presented in 7th PhDay: From Theory to Application: Research in Action, 2024.
  4. Ana G. Pedrosa; Francisco, Tânia; Tony A. Rodrigues; Maria J. Ferreira; Gerbrand J. Van der Heden van Noort; Jorge E. Azevedo. "On the extraction mechanism of monoubiquitinated PEX5 from the peroxisome". Paper presented in 8th Open European Peroxisome Meeting, 2022.
  5. Barros-Barbosa, Aurora; Ferreira, Maria João; Rodrigues, Tony A.; Pedrosa, Ana G; Grou, Cláudia P.; Pinto, Manuel P.; Fransen, Marc; Francisco, Tânia; Azevedo, Jorge E. "Reconstitution of recombinant PEX13 and PEX14 proteins into liposomes". Paper presented in 6th Open European Peroxisome Meeting (OEPM), 2018.
  6. Francisco, Tânia; Freitas, MO; Rodrigues, Tony A.; Lismont, C.; Domingues, P.; Pinto, MP.; Grou, Cláudia P.; Fransen, M.; Azevedo, Jorge E.. "Monomeric proteins are the preferential substrates of the peroxisomal protein import machinery". Paper presented in 40th FEBS, 2015.
  7. Nordgren, Marcus; Francisco, Tânia; Hennebel, Lore; Brees, Chantal; Lismont, Celien; Wang, Bo; van Veldhoven, P.P.; Azevedo, Jorge E; Fransen, Marc. "PEX5-mediated pexophagy in mammalian cells". Paper presented in Open European Peroxisome Meeting (OEPM), 2014.
  8. Francisco, Tânia. "The PEX5-mediated import pathway – insights into the cargo translocation step". Paper presented in Open European Peroxisome Meeting (OEPM), 2014.
  9. Grou, Cláudia P.; Francisco, Tânia; Rodrigues, Tony A.; Freitas, Marta O.; Pinto, Manuel P.; Carvalho, Andreia F.; Domingues, Pedro; et al. "Biochemical/biophysical properties of USP9X". Paper presented in EMBO Conference: Ubiquitin and Ubiquitin-like Proteins: from structure to function, 2013.
  10. Grou, Cláudia P.; Francisco, Tânia; Rodrigues, Tony A.; Freitas, Marta O.; Pinto, Manuel P.; Carvalho, Andreia F.; Domingues, Pedro; et al. "USP9X hydrolyzes the Ubiquitin-PEX5 thioester conjugate". Paper presented in FEBS Special Meeting – Protein quality control and Ubiquitin Systems in health and disease, 2012.
  11. Freitas, Marta O.; Francisco, Tânia; Rodrigues, Tony A; Alencastre, Inês; Pinto, Manuel P.; Grou, Cláudia P.; Carvalho, Andreia F.; et al. "The PEX5-cargo protein interaction". Paper presented in 3rd Open European Peroxisome meeting (OEPM),, 2012.
  12. Francisco, Tânia; Grou, Cláudia P.; Sá-Miranda, Clara; Pinto, Manuel P.; Azevedo, Jorge E. "Peroxisomal membrane protein sorting into peroxisomes". Paper presented in 3rd European Open Peroxisome Meeting (OEPM), 2012.
Journal article
  1. Francisco, Tânia; Pedrosa, Ana G.; Rodrigues, Tony A.; Abalkhail, Tarad; Li, Hongli; Ferreira, Maria J.; van der Heden van Noort, Gerbrand J.; et al. Corresponding author: Azevedo, Jorge E.. "Noncanonical and reversible cysteine ubiquitination prevents the overubiquitination of PEX5 at the peroxisomal membrane". PLOS Biology 22 3 (2024): e3002567. http://dx.doi.org/10.1371/journal.pbio.3002567.
    10.1371/journal.pbio.3002567
  2. Ferreira, Maria J.; Rodrigues, Tony A.; Pedrosa, Ana G.; Silva, Ana R.; Vilarinho, Beatriz G.; Francisco, Tânia; Azevedo, Jorge E.. Corresponding author: Francisco, Tânia. "Glutathione and peroxisome redox homeostasis". Redox Biology 67 (2023): 102917. http://dx.doi.org/10.1016/j.redox.2023.102917.
    10.1016/j.redox.2023.102917
  3. Maria J. Ferreira; Tony A. Rodrigues; Ana G. Pedrosa; Luís Gales; Armindo Salvador; Francisco, Tânia; Azevedo JE. Corresponding author: Francisco, Tânia. "The mammalian peroxisomal membrane is permeable to both GSH and GSSG – Implications for intraperoxisomal redox homeostasis". Redox Biology 63 (2023): 102764-102764. http://dx.doi.org/10.1016/j.redox.2023.102764.
    10.1016/j.redox.2023.102764
  4. Pedrosa, Ana G.; Francisco, Tânia; Rodrigues, Tony A.; Ferreira, Maria J.; van der Heden van Noort, Gerbrand J.; Azevedo, Jorge E.. "The Extraction Mechanism of Monoubiquitinated PEX5 from the Peroxisomal Membrane". Journal of Molecular Biology 435 2 (2023): 167896. http://dx.doi.org/10.1016/j.jmb.2022.167896.
    10.1016/j.jmb.2022.167896
  5. Ana G. Pedrosa; Katharina Reglinski; Celien Lismont; Suzan Kors; Joseph Costello; Tony A. Rodrigues; Mariana Marques; et al. "Peroxisomes: novel findings and future directions. - Meeting Report". Histochemistry and Cell Biology (2023):
    In press
  6. Dahan, Noa; Francisco, Tania; Falter, Christian; Rodrigues, Tony; Kalel, Vishal; Kunze, Markus; Hansen, Tobias; Schliebs, Wolfgang; Erdmann, Ralf. "Current advances in the function and biogenesis of peroxisomes and their roles in health and disease - Meeting Report". Histochemistry and Cell Biology 155 4 (2021): 513-524. http://dx.doi.org/10.1007/s00418-021-01982-1.
    10.1007/s00418-021-01982-1
  7. Ali, Muhammad; Khan, Shahid Y.; Rodrigues, Tony A.; Francisco, Tânia; Jiao, Xiaodong; Qi, Hang; Kabir, Firoz; et al. "A missense allele of PEX5 is responsible for the defective import of PTS2 cargo proteins into peroxisomes". Human Genetics 140 4 (2021): 649-666. http://dx.doi.org/10.1007/s00439-020-02238-z.
    10.1007/s00439-020-02238-z
  8. Pedrosa, Ana G.; Francisco, Tânia; Ferreira, Maria J.; Rodrigues, Tony A.; Barros-Barbosa, Aurora; Azevedo, Jorge E.. "A Mechanistic Perspective on PEX1 and PEX6, Two AAA+ Proteins of the Peroxisomal Protein Import Machinery". International Journal of Molecular Sciences 20 21 (2019): 5246. http://dx.doi.org/10.3390/ijms20215246.
    Open access • Published • 10.3390/ijms20215246
  9. Barros-Barbosa, Aurora; Rodrigues, Tony A.; Ferreira, Maria J.; Pedrosa, Ana G.; Teixeira, Nélson R.; Francisco, Tânia; Azevedo, Jorge E.. "The intrinsically disordered nature of the peroxisomal protein translocation machinery". The FEBS Journal 286 1 (2018): 24-38. http://dx.doi.org/10.1111/febs.14704.
    Open access • 10.1111/febs.14704
  10. Barros-Barbosa, Aurora; Ferreira, Maria J.; Rodrigues, Tony A.; Pedrosa, Ana G.; Grou, Cláudia P.; Pinto, Manuel P.; Fransen, Marc; Francisco, Tânia; Azevedo, Jorge E.. "Membrane topologies of PEX13 and PEX14 provide new insights on the mechanism of protein import into peroxisomes". The FEBS Journal 286 1 (2018): 205-222. http://dx.doi.org/10.1111/febs.14697.
    Open access • 10.1111/febs.14697
  11. Pedrosa, Ana G.; Francisco, Tânia; Bicho, Diana; Dias, Ana F.; Barros-Barbosa, Aurora; Hagmann, Vera; Dodt, Gabriele; et al. "Peroxisomal monoubiquitinated PEX5 interacts with the AAA ATPases PEX1 and PEX6 and is unfolded during its dislocation into the cytosol". Journal of Biological Chemistry 293 29 (2018): 11553-11563. http://dx.doi.org/10.1074/jbc.ra118.003669.
    Published • 10.1074/jbc.ra118.003669
  12. Rodrigues, Tony A.; Francisco, Tânia; Azevedo, Jorge E.. "PEX13 Enters the RING, Lives Fast, Dies Young. (Commentary)". Journal of Molecular Biology 430 11 (2018): 1559-1561. http://dx.doi.org/10.1016/j.jmb.2018.04.005.
    Published • 10.1016/j.jmb.2018.04.005
  13. Francisco, T.; Rodrigues, T.A.; Dias, A.F.; Barros-Barbosa, A.; Bicho, D.; Azevedo, J.E.. "Protein transport into peroxisomes: Knowns and unknowns". BioEssays 39 10 (2017): http://www.scopus.com/inward/record.url?eid=2-s2.0-85029926379&partnerID=MN8TOARS.
    10.1002/bies.201700047
  14. Dias, A.F.; Rodrigues, T.A.; Pedrosa, A.G.; Barros-Barbosa, A.; Francisco, T.; Azevedo, J.E.. "The peroxisomal matrix protein translocon is a large cavity-forming protein assembly into which PEX5 protein enters to release its cargo". Journal of Biological Chemistry 292 37 (2017): 15287-15300. http://www.scopus.com/inward/record.url?eid=2-s2.0-85029471726&partnerID=MN8TOARS.
    Published • 10.1074/jbc.M117.805044
  15. Dias, A.F.; Francisco, T.; Rodrigues, T.A.; Grou, C.P.; Azevedo, J.E.. "The first minutes in the life of a peroxisomal matrix protein". Biochimica et Biophysica Acta - Molecular Cell Research 1863 5 (2016): 814-820. http://www.scopus.com/inward/record.url?eid=2-s2.0-84964265518&partnerID=MN8TOARS.
    Published • 10.1016/j.bbamcr.2015.09.025
  16. Rodrigues, T.A.; Francisco, T.; Dias, A.F.; Pedrosa, A.G.; Grou, C.P.; Azevedo, J.E.. "A cell-free organelle-based in vitro system for studying the peroxisomal protein import machinery". Nature Protocols 11 12 (2016): 2454-2469. http://www.scopus.com/inward/record.url?eid=2-s2.0-84996598602&partnerID=MN8TOARS.
    Published • 10.1038/nprot.2016.147
  17. Nordgren, M.; Francisco, T.; Lismont, C.; Hennebel, L.; Brees, C.; Wang, B.; van Veldhoven, P.P.; Azevedo, J.E.; Fransen, M.. "Export-deficient monoubiquitinated PEX5 triggers peroxisome removal in SV40 large T antigen-transformed mouse embryonic fibroblasts". Autophagy 11 8 (2015): 1326-1340. http://www.scopus.com/inward/record.url?eid=2-s2.0-84943775216&partnerID=MN8TOARS.
    10.1080/15548627.2015.1061846
  18. Freitas, M.O.; Francisco, T.; Rodrigues, T.A.; Lismont, C.; Domingues, P.; Pinto, M.P.; Grou, C.P.; Fransen, M.; Azevedo, J.E.. "The peroxisomal protein import machinery displays a preference for monomeric substrates". Open Biology 5 4 (2015): http://www.scopus.com/inward/record.url?eid=2-s2.0-84934927612&partnerID=MN8TOARS.
    10.1098/rsob.140236
  19. Rodrigues, T.A.; Alencastre, I.S.; Francisco, T.; Brites, P.; Fransen, M.; Grou, C.P.; Azevedo, J.E.. "A PEX7-centered perspective on the peroxisomal targeting signal type 2-mediated protein import pathway". Molecular and Cellular Biology 34 15 (2014): 2917-2928. http://www.scopus.com/inward/record.url?eid=2-s2.0-84904312911&partnerID=MN8TOARS.
    10.1128/MCB.01727-13
  20. Francisco, T.; Rodrigues, T.A.; Pinto, M.P.; Carvalho, A.F.; Azevedo, J.E.; Grou, C.P.. "Ubiquitin in the peroxisomal protein import pathway". Biochimie 98 1 (2014): 29-35. http://www.scopus.com/inward/record.url?eid=2-s2.0-84895097185&partnerID=MN8TOARS.
    10.1016/j.biochi.2013.08.003
  21. Francisco, T.; Rodrigues, T.A.; Freitas, M.O.; Grou, C.P.; Carvalho, A.F.; Sá-Miranda, C.; Pinto, M.P.; Azevedo, J.E.. "A cargo-centered perspective on the PEX5 receptor-mediated peroxisomal protein import pathway". Journal of Biological Chemistry 288 40 (2013): 29151-29159. http://www.scopus.com/inward/record.url?eid=2-s2.0-84885091904&partnerID=MN8TOARS.
    10.1074/jbc.M113.487140
  22. Grou, C.P.; Francisco, T.; Rodrigues, T.A.; Freitas, M.O.; Pinto, M.P.; Carvalho, A.F.; Domingues, P.; et al. "Identification of ubiquitin-specific protease 9X (USP9X) as a deubiquitinase acting on ubiquitin-peroxin 5 (PEX5) thioester conjugate". Journal of Biological Chemistry 287 16 (2012): 12815-12827. http://www.scopus.com/inward/record.url?eid=2-s2.0-84859762823&partnerID=MN8TOARS.
    10.1074/jbc.M112.340158
  23. Freitas, M.O.; Francisco, T.; Rodrigues, T.A.; Alencastre, I.S.; Pinto, M.P.; Grou, C.P.; Carvalho, A.F.; et al. "PEX5 protein binds monomeric catalase blocking its tetramerization and releases it upon binding the N-terminal domain of PEX14". Journal of Biological Chemistry 286 47 (2011): 40509-40519. http://www.scopus.com/inward/record.url?eid=2-s2.0-81755181731&partnerID=MN8TOARS.
    10.1074/jbc.M111.287201
  24. Marques, C.J.; Francisco, T.; Sousa, S.; Carvalho, F.; Barros, A.; Sousa, M.. "Methylation defects of imprinted genes in human testicular spermatozoa". Fertility and Sterility 94 2 (2010): 585-594. http://www.scopus.com/inward/record.url?eid=2-s2.0-77953712614&partnerID=MN8TOARS.
    10.1016/j.fertnstert.2009.02.051
  25. Dória, S.; Carvalho, F.; Ramalho, C.; Lima, V.; Francisco, T.; Machado, A.P.; Brandão, O.; et al. "An efficient protocol for the detection of chromosomal abnormalities in spontaneous miscarriages or foetal deaths". European Journal of Obstetrics Gynecology and Reproductive Biology 147 2 (2009): 144-150. http://www.scopus.com/inward/record.url?eid=2-s2.0-71849084531&partnerID=MN8TOARS.
    10.1016/j.ejogrb.2009.07.023
  26. Grou, C.P.; Carvalho, A.F.; Pinto, M.P.; Alencastre, I.S.; Rodrigues, T.A.; Freitas, M.O.; Francisco, T.; Sá-Miranda, C.; Azevedo, J.E.. "The peroxisomal protein import machinery - A case report of transient ubiquitination with a new flavor". Cellular and Molecular Life Sciences 66 2 (2009): 254-262. http://www.scopus.com/inward/record.url?eid=2-s2.0-59149083150&partnerID=MN8TOARS.
    10.1007/s00018-008-8415-5
Preprint
  1. Chen, Walter W.; Rodrigues, Tony A.; Wendscheck, Daniel; Pedrosa, Ana G.; Yang, Chendong; Francisco, Tânia; Möcklinghoff, Till; et al. "PEX39 facilitates the peroxisomal import of PTS2 proteins". 2024. http://dx.doi.org/10.1101/2024.04.30.591961.
    10.1101/2024.04.30.591961
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2024/11/15 Non-conventional ubiquitination of PEX5 - a unique example of reversible ubiquitination catalyzed by a general purpose E2. Presented by Francisco, T. 11th i3S Annual Meeting
i3S (Póvoa do Varzim, Portugal)
2024/10/25 Nonconventional ubiquitination at a cysteine residue: protecting PEX5 from overubiquitination. Presented by Francisco T. XXII NATIONAL CONGRESS OF BIOCHEMISTRY
Portuguese Biochemical Society (Aveiro, Portugal)
2024/09/27 "Now you see it now you dont - Why PEX5 is ubiquitinated at a cysteine residue". Presented by Francisco T. 9th Open European Peroxisome meeting (OEPM 2024)
(St. Feliu de Guixols , Spain)
2024/09/27 "PEX39 is a conserved biogenesis factor that facilitates PTS2-protein import". Presented by Rodrigues TA 9th Open European Peroxisome meeting (OEPM 2024)
(St. Feliu de Guixols , Spain)
2024/09/22 "PEX39 is a novel PTS2 import factor and orchestrates PEX7 function" Presented by Wendscheck, D. EMBO Workshop: Advances and challenges in protein translocation across membranes
EMBO (St. Feliu de Guixols , Spain)
2023/11/17 The mammalian peroxisomal membrane is permeable to GSH and GSSG - implications for intraperoxisomal redox homeostasis. Presented by Francisco, T. 10th i3S Annual Meeting
Universidade do Porto Instituto de Investigação e Inovação em Saúde (i3S) (Póvoa do Varzim, Portugal)
2023/11/17 "The crucial role of ubiquitin in the PEX5-mediated peroxisomal protein import pathway" - Presented by Pedrosa, AG. 10th Annual Meeting of i3S
Universidade do Porto Instituto de Investigação e Inovação em Saúde (i3S) (Póvoa do Varzim, Portugal)
2023/05/08 The role of ubiquitin in the extraction of PEX5 from the peroxisomal membrane THIRD MEETING PHD IN BIOMEDICAL SCIENCES
ICBAS-UP (Porto, Portugal)
2023/03/07 Dissecting the mechanism of protein import into peroxisomes using a mammalian cell-free in vitro system. Presented by J. Azevedo
Harvard Medical School (United States)
2022/09/22 Identification and characterization of an additional PTS2 import factor. Presented by Daniel Wendscheck 8th Open European Peroxisome Meeting
(Aveiro, Portugal)
2022/09/22 Understanding the peroxisome/cytosol redox relationship. Presented by Maria J. Ferreira. 8th Open European Peroxisome Meeting
(Aveiro, Portugal)
2020/10/15 Defining the membrane topologies of PEX13 and PEX14. Presented by Maria João Ferreira 7th Open European Peroxisome meeting (OEPM) meeting
Ruhr - University Bochum - Systems Biochemistry (Zoom Conference, Germany)
2018/11/08 The peroxisomal protein import machinery: new findings and their implications. Presented by Tânia Francisco
i3S - Instituto de Investigação e Inovação em Saúde (Porto, Portugal)
2018/10/25 Monoubiquitinated PEX5 is a natural substrate of the AAA ATPases PEX1 and PEX6. Presented by Pedrosa, AG 6th Open European Peroxisome meeting (OEPM) meeting
University Groningen (Groningen, Netherlands)
2018/05/07 Dissecting the peroxisomal mechanism of protein import into peroxisomes using a biochemical in vitro approach. Presented by Pedrosa, AG 1st meeting PhD in Biomedical Sciences from Instituto de Ciências Biomédicas de Abel Salazar – Universidade do Porto (ICBAS-UP)
ICBAS - Universidade do Porto (Porto, Portugal)
2016/06/15 Translocation of proteins across the peroxisomal membrane. Presented by Azevedo, JE 5th International Iberian Biophysics Congress
(Porto, Portugal)
2015/03/25 Dissecting the peroxisomal protein import pathway using an in vitro import/export system Dissecting the peroxisomal protein import pathway using an in vitro import/export system
(Cologne, Germany)
2014/09/09 The PEX5-mediated import pathway – insights into the cargo translocation step. Presented by Tânia Francisco 4th Open European Peroxisome Meeting
2014/09/09 Mechanistic insights on PEX7 trafficking. Presented by Tony A. Rodrigues 4th Open European Peroxisome Meeting
2014/09/09 PEX5-mediated pexophagy in mammalian cells. Presented by Nordgren M. 4th Open European Peroxisome Meeting
2012/07/05 “Identification of a mammalian deubiquitinase hydrolyzes the soluble Ub-PEX5 thioester conjugate". Presented Grou CP. 5th Open European Peroxisome Meeting
2007/11/15 An Efficient Protocol for detection of chromosomal abnormalities in spontaneous abortions. Presented by Doria S. 11º Reunião da Sociedade Portuguesa de Genética Humana

Supervision

Thesis Title
Role
Degree Subject (Type)
Institution / Organization
2023/01/02 - 2023/07/25 Biochemistry bachelor's degree internship - Identificação/Caraterização dos Componentes Moleculares Envolvidos na Pexofagia
Co-supervisor
Bioquímica (Degree)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2018/10 - 2022/11/09 Dissecting the mechanisms of peroxisomal ROS homeostasis
Co-supervisor of Maria João Marques de Campos Ferreira
Ciências Biomédicas (PhD)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2017/09 - 2022 Dissecting the receptor export module of the peroxisomal protein import pathway
Co-supervisor of Ana Margarida Ferreira Guimarães Pedrosa
Ciências Biomédicas (PhD)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2020/10 - 2021/09 Biology bachelor's degree intership - Caracterização de um possível novo componente da maquinaria de importação proteica peroxissomal
Supervisor of João Marcos Freitas Vieira
Biology (Degree)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal

Universidade do Porto Faculdade de Ciências, Portugal
2020/12 - 2021/07 Biochemistry bachelor's degree internship - CHARACTERIZATION OF A PUTATIVE NEW PLAYER IN PEROXISOMAL PROTEIN IMPORT
Co-supervisor of Bernardo Silva
Bachelor's degree internship (Degree)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal

Universidade do Porto Faculdade de Ciências, Portugal
2017/10 - 2018/12/11 O mecanismo autorregulatório da PEX5, o recetor para a importação de proteínas da matriz peroxissomal
Co-supervisor of Nélson Rodrigo dos Reis Teixeira
Bioquímica (Master)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal

Universidade do Porto Faculdade de Ciências, Portugal
2015/10 - 2016/12 The peroxisomal protein import pathway: a functional study of the N-terminal half of PEX5
Co-supervisor of Ana Margarida Pedrosa
Bioquímica (Master)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal

Universidade do Porto Faculdade de Ciências, Portugal

Event organisation

Event name
Type of event (Role)
Institution / Organization
2023/02/02 - Current spokesperson at i3S educational exhibitions - i3S MSc Open Day 2023 (2023/02/02)
Exhibition (Other)
2022 - 2023 Science embassador of the Educational Programme of i3S: CienciaEtal (cienciaetal.i3s.up.pt). Participation in the Workshop ¿A Herança dos Maias¿ (where we use molecular biology activities in their classrooms to promote genetic and hereditary concepts,) and in career (or scientific) talks (either for visiting students or at their schools). (2022)
Other (Co-organisor)
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2021/02 - 2022/09/30 Co-Organizer of the 8th Open European Peroxisome Meeting (2022/09/22 - 2022/09/24)
Conference (Member of the Scientific Committee)
Universidade do Porto, Portugal

Universidade de Aveiro, Portugal
2018/12/12 - 2018/12/14 Co-organizer of the "Career Development Workshop for researchers in the biosciences", 12-14 of December 2018, A 2-days hands-on and personalized workshop with two tutors from the Imperial College (London), to promote the development and professional advancement of early career researchers (2018/12/12 - 2018/12/14)
Workshop (Member of the Organising Committee)
2018/01/09 - 2018/11/30 Co-organizer of the "Workshop Moving to Industry" 9, 30 and 31 October 2018 | i3S 3 day Workshop on personal and professional development for researchers (PhDs) in the Bioscience field, focused on two main career planning processes when an academia-industry transition is desired. (2018/10/09 - 2018/10/31)
Workshop (Member of the Organising Committee)
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2018/01/15 - 2018/01/16 Co-organizer of the ¿Effective Communication workshop¿. 2-days workshop on effective communication - a hands-on course on personal and professional development for researchers (PhDs) and PhD students. (2018/01/15 - 2018/01/16)
Workshop (Member of the Organising Committee)
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2017/05/29 - 2017/05/29 Co-organizer of the ¿Writing your way into the right job - a CV skills workshop¿ - Hands-on 1-day workshop on personal and professional development for researchers (PhDs) and PhD students in the Biosciences. (2017/05/29 - 2017/05/29)
Workshop (Co-organisor)
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2016/10/20 - 2016/10/21 Co-organizer of "Careers in Science & Beyond" - two-day workshop at the i3S, Instituto de Investigação e Inovação em Saúde, dedicated to career development for young researchers. (2016/10/20 - 2016/10/21)
Workshop
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2008 - 2015 Participation in the Educational Programme of the Associated Laboratory IBMC/INEB. Organizing and hosting lab visits for undergraduated students (secondary education students). (2008)
Other (Co-organisor)
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2010/06/17 - 2010/06/17 Co-organizer of the Symposium on career development “Careers in Science: Life After a PhD", June 17, 2010 (2010/06/17 - 2010/06/17)
Symposium (Co-organisor)
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal

Event participation

Activity description
Type of event
Event name
Institution / Organization
2020/10/15 - 2020/10/16 Chair
Conference
7th Open European Peroxisome meeting (OPEM) meeting
Ruhr-Universität Bochum, Germany
2012/07/05 - 2012/07/06 Chair
Conference
3rd Open European Peroxisome meeting (OPEM) meeting

Jury of academic degree

Topic
Role
Candidate name (Type of degree)
Institution / Organization
2022/12/15 Deciphering the crosstalk between MAVS- and STING- dependent antiviral responses
(Thesis) Main arguer
Rui Filipe Tavares Ferreira (Master)
Universidade de Aveiro, Portugal
2022/11 Yck2: At The Intersection Of Prion-like Domains And Stress Granules Regulation?
(Thesis) Main arguer
Eduarda R F Pinto (Master)
Universidade do Porto, Portugal
2021/12 Novel tools to study the interplay between peroxisomes and lipid droplets in hepatitis C virus infection
(Thesis) Main arguer
Ariana dos Santos Carvalho (Master)
Universidade de Aveiro, Portugal

Ad Hoc journal article review

Journal title (ISSN) Publisher
2022 - Current Cells MDPI
2020/03 - 2020/03 Biomolecules
2019/06 - 2019/08 Scientific Reports

Conference scientific committee

Conference name Conference host
2022/05 - 2023/09 8th Open European Peroxisome Meeting Universidade do Porto; Universidade de Aveito
2022 - 2023/05/08 Third meeting PhD in Biomedical Sciences from Instituto de Ciências Biomédicas de Abel Salazar ¿ Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar - Universidade do Porto
2018/05 - 2018/05 First meeting PhD in Biomedical Sciences from Instituto de Ciências Biomédicas de Abel Salazar ¿ Universidade do Porto Instituto de Ciências Biomédicas de Abel Salazar – Universidade do Porto

Course / Discipline taught

Academic session Degree Subject (Type) Institution / Organization
2024/09 - Current Complements of Molecular Biology - Master in Biochemistry ICBAS-UP Complements of Molecular Biology (Mestrado) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2022/02 - Current Protein Structure and Function - Bachelor in Bioengineering (ICBAS-UP) Ciências de Engenharia, orientação em Bioengenharia (Licenciatura) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2020/02 - Current Protein Structure and Function - Master in Biochemistry ICBAS-UP Bioquímica (Mestrado integrado) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2020/02 - Current Protein Structure and Function - Master in Bioengeneering ICBAS-UP Bioengenharia (Mestrado integrado) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2018/09 - Current Biochemistry and Metabolism - Master in Medicine (ICBAS -UP) Medicina (Mestrado integrado) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2017/09 - 2018/02 Biochemistry - Master in Medicine (ICBAS -UP) Medicina (Mestrado integrado) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal

Evaluation committee

Activity description
Role
Institution / Organization Funding entity
2023/01 - 2023/01 Member of the jury for a research fellowship (PhD level) within the project "A new component of the mammalian peroxisomal protein import machinery - 2022.08378.PTDC", BIPD/i3S/17013001/2023, January 2023
Evaluator
2023/01 - 2023/01 Member of the jury for a research fellowship (MSc level) within the project "Investigating the molecular mechanisms of mammalian pexophagy - EXPL/BIA-MOL/1664/2021”, BI/FCT_Proj2021/i3S/14120601/2022, January, 2023
2022/09 - 2022/10 Evaluator and part of the Selection Committee for the international PhD Programme for Medical Doctors, EMERALD, an EU-funded MSCA project with the aim of recruiting and training MDs that want to do a PhD in one of the EMERALD research centres.
Evaluator
2017/11/01 - 2017/11/15 Member of the jury for a research fellowship (MSc level) within the project NORTE-01-0145-FEDER-000008 – Porto Neurosciences and Neurologic Disease Research Initiative at i3S. Norte2020NEURO59 November, 2017.
Evaluator
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal

Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
Fundação para a Ciência e a Tecnologia
2017/09/22 - 2017/10/30 Member of the jury for a research fellowship (MSc level) within the project NORTE-01-0145-FEDER-000008 – Porto Neurosciences and Neurologic Disease Research Initiative at i3S. Norte2020NEURO57 October, 2017.
Evaluator
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal

Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
Fundação para a Ciência e a Tecnologia
2017/04 - 2017/04 member of the jury for a research fellowship (MSc level) under the project “NORTE-01-0145-FEDER- 000008 – Porto Neurosciences and Neurologic Disease Research Initiative Norte2020NEURO47 (April 2017)
Evaluator
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal

Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
Fundação para a Ciência e a Tecnologia
2017/01/24 - 2017/02/10 member of the jury for a research fellowship (MSc level) under the project “NORTE-01-0145-FEDER- 000008 – Porto Neurosciences and Neurologic Disease Research Initiative" Norte2020NEURO38 (February, 2017)
Evaluator
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal

Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
Fundação para a Ciência e a Tecnologia
2016/06/24 - 2016/07/07 member of the jury for a research fellowship (Pos-doctoral level) under the project “PTDC/BEX- BCM/2311/2014 - The molecular mechanisms of peroxisome biogenesis” at IBMC/i3S – PR27201601 June, 2016.
Evaluator
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal

Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
Fundação para a Ciência e a Tecnologia
2016/03/15 - 2016/03/30 Member of the jury for a research fellowship (Pos-Doctoral level) within the project NORTE-01-0145-FEDER- 000008 – Porto Neurosciences and Neurologic Disease Research Initiative at IBMC/i3S. Norte2020NEURO19 March, 2016.
Evaluator
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal

Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
Fundação para a Ciência e a Tecnologia
2015/02/09 - 2015/02/21 Member of the jury for a research fellowship (MSc level) within the project FCOMP-01-0124-FEDER- 019731 - PTDC/BIA-BCM/118577/2010 - Mecanismo molecular da importação de proteínas para o peroxissoma. PR271501 February, 2015
Evaluator
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal

Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2014/08/21 - 2014/09/03 Member of the jury for a research fellowship (MSc level) within the project FCOMP-01-0124-FEDER- 019731 - PTDC/BIA-BCM/118577/2010 - Mecanismo molecular da importação de proteínas para o peroxissoma. PR271402 September, 2014.
Evaluator
Fundação para a Ciência e a Tecnologia
2013/09 - 2013/09 Member of the jury for a research fellowship (MSc level) within the project FCOMP-01-0124-FEDER- 019731 - PTDC/BIA-BCM/118577/2010 - Mecanismo molecular da importação de proteínas para o peroxissoma. PR270102 September, 2013.
Evaluator
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal

Journal scientific committee

Journal title (ISSN) Publisher
2023/09/29 - Current Review Editor of Frontiers in Cell and Developmental Biology - Membrane Traffic MDPI
2020/12 - 2022 Topic Editor of Frontiers in Cell and Developmental Biology Frontiers
Distinctions

Other distinction

2013 Post-Doctoral Fellowship
Fundação para a Ciência e a Tecnologia, Portugal
2009 PhD fellowship
Fundação para a Ciência e a Tecnologia, Portugal