???global.info.a_carregar???
Fani Sousa completed the Ph.D. in Biochemistry in 2008 and is Associate professor at the University of Beira Interior. Sousa F was Vice-Coordinator of CICS-UBI - Health Sciences Research Center at the University of Beira Interior between 2017 and 2021, and Coordinator of the Biopharmaceuticals and Biomaterials research group at CICS-UBI from 2018 to 2021. Sousa F was also the Coordinator of the Biotechnology and Biomolecular Sciences research group between 2010-2014. At a scientific level, her main research activity is related to Health Biotechnology, including the development of new biotechnological platforms for the production, recovery, purification, and stabilization of biological products for therapeutic application (biopharmaceuticals). Sousa F participated in 14 funded research projects and is/was a principal investigator in 4 projects in this field. Among these, it was recently approved a European Research Project (HORIZON-EIC-2021-PATHFINDEROPEN-01-01), where Sousa F is the Principal Investigator at UBI (Participant Institution). The national and international network of Sousa F, with academia and industry, is supported by joint publications, supervision of students, and projects in collaboration. Sousa F has experience in students supervision, with 52 master and 8 PhD theses completed, and she is currently supervising / co-supervising 6 doctoral students and 6 master students. Fani Sousa has published more than 130 scientific articles in international peer-reviewed journals, 6 book chapters and 1 patent (+2300 citations; h-index 28).
Read full resume ↓
Identification

Personal identification

Full name
Fani Pereira de Sousa

Citation names

  • Sousa, F

Author identifiers

Ciência ID
991D-2E13-A840
ORCID iD
0000-0001-9996-2194
Researcher Id
A-2014-2017
Scopus Author Id
7005110268

Email addresses

  • fani.sousa@fcsaude.ubi.pt (Professional)

Addresses

  • CICS-UBI - Health Sciences Reasearch Centre, University of Beira Interior, Av. Infante D. Henrique, 6200-506, Covilhã, Covilhã, Portugal (Professional)

Knowledge fields

  • Medical and Health Sciences - Medical Biotechnology - Health-Related Biotechnology
  • Medical and Health Sciences - Medical Biotechnology - Gene-based Diagnostics and Therapeutic Interventions
Education
Degree Classification
2008
Concluded
 Biochemistry (Doutoramento)
Universidade da Beira Interior, Portugal
"Affinity chromatographic processes in nucleic acids" (THESIS/DISSERTATION)
 Approved
2003
Concluded
 Biochemistry (Licenciatura)
Universidade da Beira Interior, Portugal
"Production and Recovery of a fusion protein containing the AD-1 domain of Human Citomagalovirus" (THESIS/DISSERTATION)
 15
Affiliation

Science

Category
Host institution 		Employer
2005/09/01 - 2008/09/03 Researcher (Research) Universidade da Beira Interior, Portugal
2003/10/29 - 2005/08/30 Researcher (Research) Universidade da Beira Interior, Portugal

Teaching in Higher Education

Category
Host institution 		Employer
2021 - Current Associate Professor (University Teacher) Universidade da Beira Interior, Portugal
2009 - 2021/01/31 Assistant Professor (University Teacher) Universidade da Beira Interior, Portugal
2008 - 2009 Invited Assistant Professor (University Teacher) Universidade da Beira Interior, Portugal

Others

Category
Host institution 		Employer
2018/01/12 - 2021 Coordinator of the Research Group: Biopharmaceuticals and Biomaterials - Health Sciences Research Centre Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2017 - 2021 Vice-coordinator of the Health Sciences Research Centre Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2017/03/09 - 2018/01/11 Coordinator of the Research Group of Biotechnology and Biomolecular Sciences - Health Sciences Research Centre Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2010/03/10 - 2014/10/31 Coordinator of the Research Group of Biotechnology and Biomolecular Sciences - Health Sciences Research Centre Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
Projects

Contract

Designation Funders
2021 - Current Evaluation of novel chromatography prototypes for the purification of nucleic-acids-based therapeutics
-
Principal investigator
Merck KGaA
Ongoing
2022/04 - 2025/03 Y-Script - Yeast cell factory for mRNA bioproduction
EU Project 101047214
Principal investigator
European Commission
Ongoing
2021 - 2024 CICS4COVID - Testar, Rastrear, e Inovar no Diagnóstico da COVID-19
72541
Researcher
Concluded
2022/06 - 2023/06 PAPILOMA
CENTRO-01-0145-FEDER-181235
Researcher
Comissão de Coordenação e Desenvolvimento Regional do Centro, Portugal
Concluded
2018 - 2022/06 PUREmiRSILs - Ionic Liquid-based supports for pre-miRNAs purification targeting Alzheimer's disease
POCI-01-0145-FEDER-029496
Principal investigator
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2020 - 2021 CovidOUT -Spray para aplicação em máscaras e no nariz para reduzir a transmissão do novo coronavírus
69358
Researcher
Concluded
2018 - 2021 IL2BioPro - Toward an improvement of the recombinant proteins bioprocessing using ionic liquids
POCI-01-0145-FEDER-030840
Researcher
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2018 - 2021 Research Line 2 - Cloud Applications, WP 2.6 – Bioinformatics
C4- CLOUD COMPUTING COMPETENCE CENTRE
Researcher
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
Concluded
2018 - 2020 ICON - Desafios interdisciplinares em neurodegeneração
CENTRO-01-0145-FEDER-000013
Researcher
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
 Comissão de Coordenação e Desenvolvimento Regional do Centro
Concluded
2018 - 2019 Pre-miRNAs for Alzheimer's disease therapy: biosynthesis, purification and delivery
4329/40839UA
Principal investigator
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2018 - 2019 BIOmedical DEVICE development based on pre-miRNA G-quadruplex
MIT-EXPL/BIO/0008/2017
Researcher
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2013 - 2015 Technologies for purification and controlled release of biopharmaceuticals to be applied in age-related diseases
CENTRO-07-ST24-FEDER-002014
Principal investigator
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
 Comissão de Coordenação e Desenvolvimento Regional do Centro
Concluded
2013 - 2014 Pre-miR-29 bioseparation and target delivery strategies for Alzheimer's Disease
EXPL/BBB-BIO/1056/2012
Principal investigator
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2013 - 2014 Biosynthesis, Purification and Biointeraction of human soluble catechol-O-metiltransferase with Parkinson´s inhibitors
EXPL/BBB-BQB/0960/2012
Researcher
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2011 - 2014 User-friendly model to simulate single and multi-component biomacromolecule adsorption onto ion-exchange and hydrophobic interaction supports
PTDC/EBB-BIO/113576/2009
Researcher
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2010 - 2014 Isolation and Purification of Plasmid DNA for Cancer Therapy
PTDC/EBB-BIO/114320/2009
Researcher
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2010 - 2013 Interacções de Afinidade entre Cianinas e Biomoléculas em Processos Cromatográficos
PTDC/QUI-QUI/100896/2008
Researcher
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2007 - 2009 Synthesis, optimization and intensification of bioseparation processes for DNA vaccine manufacturing
PTDC/EQU-EQU/65492/2006
Researcher
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2003 - 2005 Estudo da Infeção Congénita pelo Citomegalovírus Humano
Programa SAÚDE XXI
Research Fellow
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
Concluded
Outputs

Publications

Book chapter
  1. Carapito AR; Valente JFA; Queiroz JA; Sousa, F. Corresponding author: Sousa, F. "Arginine-Affinity Chromatography for Nucleic Acid (DNA and RNA) Isolation". In Affinity Chromatography. Methods in Molecular Biology. Humana, 2022.
    Published
  2. Almeida AM; Eusébio D; Queiroz JA; Sousa, F; Sousa Â. "Minicircle DNA Vaccine Purification and E7 Antigen Expression Assessment". In DNA Vaccines, 207-222. Humana, 2021.
    Published
  3. Costa D; Sousa, F; Queiroz JA. "Development of plasmid DNA nanoparticles for mitochondrial gene therapy". In In Materials for Biomedical Engineering - Bioactive Materials for Antimicrobial, Anticancer, and Gene Therapy, 297-327. Elsevier, 2019.
    Published
  4. Gaspar, V.M.; Moreira, A.F.; de Melo-Diogo, D.; Costa, E.C.; Queiroz, J.A.; Sousa, F.; Pichon, C.; Correia, I.J.. "Multifunctional nanocarriers for codelivery of nucleic acids and chemotherapeutics to cancer cells". 2016.
    10.1016/B978-0-323-41736-5.00006-6
  5. Sousa, Â.; Queiroz, J.A.; Sousa, F.. "p53-encoding pDNA purification by affinity chromatography for cancer therapy". 2015.
    10.1007/978-1-4939-2727-2_7
  6. Graça, V.C.; Sousa, F.; Santos, P.F.; Almeida, P.S.. "Protein purification by aminosquarylium cyanine dye-affinity chromatography". 2015.
    10.1007/978-1-4939-2447-9_2
Journal article
  1. Ferreira, Pedro L.; Marie, Helena; Berger, Tim; Edelmann, Bianca; Rammo, Oliver; Sousa, Fani. "Evaluation of novel chromatographic prototypes for supercoiled plasmid DNA polishing". Frontiers in Bioengineering and Biotechnology 11 (2024): http://dx.doi.org/10.3389/fbioe.2023.1296444.
    10.3389/fbioe.2023.1296444
  2. P. Ferreira; M. Riscado; S. Bernardo; M.G. Freire; J.L. Faria; APM. Tavares; CG. Silva; F. Sousa. "Pristine Multi-walled carbon nanotubes for a rapid and efficient plasmid DNA clarification". Separation and Purification Technology (2023): https://doi.org/10.1016/j.seppur.2023.124224.
    10.1016/j.seppur.2023.124224
  3. Rita Carapito; Sandra C. Bernardo; Matheus M. Pereira; Márcia C. Neves; Mara G. Freire; Fani Sousa. "Multimodal ionic liquid-based chromatographic supports for an effective RNA purification". Separation and Purification Technology (2023): https://doi.org/10.1016/j.seppur.2023.123676.
    10.1016/j.seppur.2023.123676
  4. Dinis, Teresa B.V.; Valente, Ana I.; Tavares, Ana P.M.; Sousa, Fani; Freire, Mara G.. "Integrated platform resorting to ionic liquids comprising the extraction, purification and preservation of DNA". Separation and Purification Technology 315 (2023): 123646. http://dx.doi.org/10.1016/j.seppur.2023.123646.
    10.1016/j.seppur.2023.123646
  5. Baptista, Bruno; Oliveira, Andreia S.R.; Mendonça, Patrícia; Serra, Arménio C.; Coelho, Jorge F.J.; Sousa, Fani. "pH-responsive nanoparticles based on POEOMA-b-PDPA block copolymers for RNA encapsulation, protection and cell delivery". Biomaterials Advances 145 (2023): 213267. http://dx.doi.org/10.1016/j.bioadv.2022.213267.
    10.1016/j.bioadv.2022.213267
  6. Joana F. A. Valente; Tiago Soares Carreira; Juliana R. Dias; Fani Sousa; Nuno Alves. "Arginine-Modified 3D-Printed Chromatographic Supports". Pharmaceutics (2022): https://doi.org/10.3390/pharmaceutics14112266.
    10.3390/pharmaceutics14112266
  7. Joana Valente; JR Dias; Sousa F; Nuno Alves. "3D Printing for Affinity Chromatographic Support Production". Materials Proceedings (2022): https://www.mdpi.com/2673-4605/8/1/82.
    10.3390/materproc2022008082
  8. Cardoso, Sara; Sousa, Fani; Pessoa Filho, Pedro A.; R. Azzoni, Adriano. "Understanding the adsorption of plasmid DNA and RNA molecules onto arginine-agarose chromatographic resin". Molecular Biology Reports 49 5 (2022): 3893-3901. http://dx.doi.org/10.1007/s11033-022-07239-x.
    10.1007/s11033-022-07239-x
  9. Ângela Sousa; Ana Margarida Almeida; Joana Valente; João Queiroz; Fani Sousa. "Hands-On Laboratory Class for Biopharmaceutical pDNA Quality Control". Journal of Chemical Education (2022): https://doi.org/10.1021/acs.jchemed.1c00695.
    10.1021/acs.jchemed.1c00695
  10. Bruno Baptista; Rita Carapito; Nabila Laroui; Chantal Pichon; Fani Sousa. "mRNA, a Revolution in Biomedicine". Pharmaceutics (2021): https://doi.org/10.3390/pharmaceutics13122090.
    10.3390/pharmaceutics13122090
  11. Josué Carvalho; Jéssica Lopes-Nunes; Joana Figueiredo; Tiago Santos; André Miranda; Micaela Riscado; Fani Sousa; et al. "Molecular Beacon Assay Development for Severe Acute Respiratory Syndrome Coronavirus 2 Detection". Sensors 21 21 (2021): 7015-7015. https://doi.org/10.3390/s21217015.
    10.3390/s21217015
  12. Teresa B. V. Dinis; Francisca A. e Silva; Fani Sousa; Mara G. Freire. "Advances Brought by Hydrophilic Ionic Liquids in Fields Involving Pharmaceuticals". Materials 14 21 (2021): 6231-6231. https://doi.org/10.3390/ma14216231.
    10.3390/ma14216231
  13. J.F.A. Valente; F. Sousa; N. Alves. "Additive Manufacturing Tools to Improve the Performance of Chromatographic Approaches". Trends in Biotechnology (2021): https://doi.org/10.1016/j.tibtech.2021.03.008.
    10.1016/j.tibtech.2021.03.008
  14. Patrícia Pereira; Augusto Q. Pedro; Márcia C. Neves; João C. Martins; Inês Rodrigues; Mara G. Freire; Sousa F. "Efficient Isolation of Bacterial RNAs Using Silica-Based Materials Modified with Ionic Liquids". Life (2021): https://www.mdpi.com/2075-1729/11/10/1090.
    10.3390/life11101090
  15. Micaela Riscado; Bruno Baptista; Fani Sousa. "New RNA-Based Breakthroughs in Alzheimer’s Disease Diagnosis and Therapeutics". Pharmaceutics (2021): https://doi.org/10.3390/pharmaceutics13091397.
    10.3390/pharmaceutics13091397
  16. J.F.A. Valente; P. Pereira; A. Sousa; J.A. Queiroz; F. Sousa. "Effect of Plasmid DNA Size on Chitosan or Polyethyleneimine Polyplexes Formulation". Polymers (2021): https://doi.org/10.3390/polym13050793.
    10.3390/polym13050793
  17. B. Baptista; M. Riscado; J.A. Queiroz; C. Pichon; F. Sousa. "Non-coding RNAs: Emerging from the discovery to therapeutic applications". Biochemical Pharmacology (2021): 114469-114469. https://doi.org/10.1016/j.bcp.2021.114469.
    10.1016/j.bcp.2021.114469
  18. J.F.A. Valente; J.A. Queiroz; F. Sousa. "Dilemma on plasmid DNA purification: binding capacity vs selectivity". Journal of Chromatography A 1637 (2021): 461848-461848. https://doi.org/10.1016/j.chroma.2020.461848.
    10.1016/j.chroma.2020.461848
  19. Patrícia Pereira; Maria Barreira; Carla Cruz; Joana Tomás; Ângelo Luís; Augusto Q. Pedro; João A. Queiroz; Fani Sousa. "Brain-Targeted Delivery of Pre-miR-29b Using Lactoferrin-Stearic Acid-Modified-Chitosan/Polyethyleneimine Polyplexes". Pharmaceuticals 13 10 (2020): 314-314. https://doi.org/10.3390/ph13100314.
    10.3390/ph13100314
  20. Ana Margarida Almeida; João António Queiroz; Fani Sousa; Ângela Sousa. "Minicircle DNA: The Future for DNA-Based Vectors?". Trends in Biotechnology (2020): https://doi.org/10.1016/j.tibtech.2020.04.008.
    10.1016/j.tibtech.2020.04.008
  21. M.C. Neves; P. Pereira; A.Q. Pedro; J.C. Martins; T. Trindade; J.A. Queiroz; M.G. Freire; F. Sousa. "Improved ionic-liquid-functionalized macroporous supports able to purify nucleic acids in one step". Materials Today Bio 8 (2020): 100086-100086. https://doi.org/10.1016/j.mtbio.2020.100086.
    10.1016/j.mtbio.2020.100086
  22. Almeida, A.M.; Cernigoj, U.; Queiroz, J.A.; Sousa, F.; Sousa, A.. "Quality assessment of supercoiled minicircle DNA by cadaverine-modified analytical chromatographic monolith". Journal of Pharmaceutical and Biomedical Analysis 180 (2020): 113037. http://dx.doi.org/10.1016/j.jpba.2019.113037.
    Published • 10.1016/j.jpba.2019.113037
  23. A.M. Almeida; D. Eusébio; J.A. Queiroz; F. Sousa; A. Sousa. "The use of size-exclusion chromatography in the isolation of supercoiled minicircle DNA from Escherichia coli lysate". Journal of Chromatography A 1609 (2020): 460444-460444. https://doi.org/10.1016/j.chroma.2019.460444.
    10.1016/j.chroma.2019.460444
  24. J.F.A. Valente; A. Sousa; G.A. Azevedo; J.A. Queiroz; F. Sousa. "Purification of supercoiled p53-encoding plasmid using an arginine-modified macroporous support". Journal of Chromatography A (2020): 460890-460890. https://doi.org/10.1016/j.chroma.2020.460890.
    10.1016/j.chroma.2020.460890
  25. Azevedo, G.M.; Valente, J.F.A.; Sousa, A.; Pedro, A.Q.; Pereira, P.; Sousa, F.; Queiroz, J.A.. "Effect of Chromatographic Conditions on Supercoiled Plasmid DNA Stability and Bioactivity". Applied Sciences 9 23 (2019): 5170. http://dx.doi.org/10.3390/app9235170.
    Published • 10.3390/app9235170
  26. Almeida, Ana M.; Queiroz, João A.; Sousa, Fani; Sousa, Ângela. "Cervical cancer and HPV infection: ongoing therapeutic research to counteract the action of E6 and E7 oncoproteins". Drug Discovery Today 24 10 (2019): 2044-2057. http://dx.doi.org/10.1016/j.drudis.2019.07.011.
    Published • 10.1016/j.drudis.2019.07.011
  27. Santos, Tiago; Pereira, Patrícia; Queiroz, João A.; Cruz, Carla; Sousa, Fani. "Plasmid production and purification: An integrated experiment-based biochemistry and biotechnology laboratory course". Biochemistry and Molecular Biology Education 47 6 (2019): 638-643. http://dx.doi.org/10.1002/bmb.21290.
    Published • 10.1002/bmb.21290
  28. Carvalho, Josué; Santos, Tiago; Carrilho, Rui; Sousa, Fani; Salgado, Gilmar F.; Queiroz, João António; Cruz, Carla. "Ligand screening to pre-miRNA 149 G-quadruplex investigated by molecular dynamics". Journal of Biomolecular Structure and Dynamics (2019): 1-11. http://dx.doi.org/10.1080/07391102.2019.1632743.
    Published • 10.1080/07391102.2019.1632743
  29. Maria V. Quental; Augusto Q. Pedro; Patrícia Pereira; Mukesh Sharma; João A. Queiroz; João A. P. Coutinho; Fani Sousa; Mara G. Freire. "Integrated Extraction-Preservation Strategies for RNA Using Biobased Ionic Liquids". ACS Sustainable Chemistry & Engineering (2019): https://doi.org/10.1021/acssuschemeng.9b00688.
    10.1021/acssuschemeng.9b00688
  30. J.F.A. Valente; A. Sousa; J.A. Queiroz; F. Sousa. "DoE to improve supercoiled p53-pDNA purification by O-phospho-l-tyrosine chromatography". Journal of Chromatography B 1105 (2019): 184-192. https://doi.org/10.1016/j.jchromb.2018.12.002.
    10.1016/j.jchromb.2018.12.002
  31. Almeida, A.M.; Queiroz, J.A.; Sousa, F.; Sousa, A.. "Minicircle DNA purification: Performance of chromatographic monoliths bearing lysine and cadaverine ligands". Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 1118-1119 (2019): 7-16. http://www.scopus.com/inward/record.url?eid=2-s2.0-85064324749&partnerID=MN8TOARS.
    10.1016/j.jchromb.2019.04.024
  32. Augusto Q. Pedro; Patrícia Pereira; Maria J. Quental; André P. Carvalho; Sérgio M. Santos; João A. Queiroz; Fani Sousa; Mara G. Freire. "Cholinium-Based Good’s Buffers Ionic Liquids as Remarkable Stabilizers and Recyclable Preservation Media for Recombinant Small RNAs". ACS Sustainable Chemistry & Engineering (2018): https://doi.org/10.1021/acssuschemeng.8b03900.
    10.1021/acssuschemeng.8b03900
  33. Valente, J.F.A.; Sousa, A.; Gaspar, V.M.; Queiroz, J.A.; Sousa, F.. "The biological performance of purified supercoiled p53 plasmid DNA in different cancer cell lines". Process Biochemistry 75 (2018): 240-249. http://dx.doi.org/10.1016/j.procbio.2018.09.014.
    Published • 10.1016/j.procbio.2018.09.014
  34. Valente, Joana F.A.; Queiroz, João A.; Sousa, Fani. "p53 as the Focus of Gene Therapy: Past, Present and Future". Current Drug Targets 19 15 (2018): 1801-1817. http://dx.doi.org/10.2174/1389450119666180115165447.
    Published • 10.2174/1389450119666180115165447
  35. Joana R. Góis; Fábio Reis; Ana M. Almeida; Patrícia Pereira; Fani Sousa; Arménio C. Serra; Jorge F.J. Coelho. "Preparation of well-defined brush-like block copolymers for gene delivery applications under biorelevant reaction conditions". Colloids and Surfaces B: Biointerfaces 169 (2018): 107-117. https://doi.org/10.1016/j.colsurfb.2018.05.004.
    10.1016/j.colsurfb.2018.05.004
  36. Sara Cardoso; Pedro de Alcântara Pessoa Filho; Fani Sousa; Adriano Rodrigues Azzoni. "Arginine and di-arginine ligands for plasmid DNA purification using negative chromatography". Separation and Purification Technology 202 (2018): 281-289. https://doi.org/10.1016/j.seppur.2018.03.066.
    10.1016/j.seppur.2018.03.066
  37. Sara Cardoso; Ângela Sousa; João A. Queiroz; Adriano R. Azzoni; Fani Sousa. "Arginine homopeptides for plasmid DNA purification using monolithic supports". Journal of Chromatography B 1087-1088 (2018): 149-157. https://doi.org/10.1016/j.jchromb.2018.04.031.
    10.1016/j.jchromb.2018.04.031
  38. Almeida, Ana M.; Tomás, Joana; Pereira, Patrícia; Queiroz, João A.; Sousa, Fani; Sousa, Ângela. "HPV-16 targeted DNA vaccine expression: The role of purification". Biotechnology Progress 34 2 (2018): 546-551. http://dx.doi.org/10.1002/btpr.2603.
    Published • 10.1002/btpr.2603
  39. Amorim, Lúcia F. A.; Gaspar, Rita; Pereira, Patrícia; Cernigoj, Urh; Sousa, Fani; Queiroz, João António; Sousa, Ângela. "Chromatographic HPV-16 E6/E7 plasmid vaccine purification employing L-histidine and 1-benzyl-L-histidine affinity ligands". ELECTROPHORESIS 38 22-23 (2017): 2975-2980. http://dx.doi.org/10.1002/elps.201700147.
    Published • 10.1002/elps.201700147
  40. Pereira, Patrícia; Pedro, Augusto Q.; Queiroz, João A.; Figueiras, Ana R.; Sousa, Fani. "New insights for therapeutic recombinant human miRNAs heterologous production: Rhodovolum sulfidophilum vs Escherichia coli". Bioengineered 8 5 (2017): 670-677. http://dx.doi.org/10.1080/21655979.2017.1284710.
    Published • 10.1080/21655979.2017.1284710
  41. Gaspar, Vítor M.; Cruz, Carla; Queiroz, João A.; Pichon, Chantal; Correia, Ilídio J.; Sousa, Fani. "Highly selective capture of minicircle DNA biopharmaceuticals by a novel zinc-histidine peptide conjugate". Separation and Purification Technology 174 (2017): 417-424. http://dx.doi.org/10.1016/j.seppur.2016.10.054.
    10.1016/j.seppur.2016.10.054
  42. Coutinho, Eduarda; Batista, Cátia; Sousa, Fani; Queiroz, João; Costa, Diana. "Mitochondrial Gene Therapy: Advances in Mitochondrial Gene Cloning, Plasmid Production, and Nanosystems Targeted to Mitochondria". Molecular Pharmaceutics 14 3 (2017): 626-638. http://dx.doi.org/10.1021/acs.molpharmaceut.6b00823.
    Published • 10.1021/acs.molpharmaceut.6b00823
  43. Ferreira, João; Santos, Tiago; Pereira, Patrícia; Corvo, Marta C.; Queiroz, João A.; Sousa, Fani; Cruz, Carla; et al. "Naphthalene amine support for G-quadruplex isolation". The Analyst 142 16 (2017): 2982-2994. http://dx.doi.org/10.1039/c7an00648a.
    Published • 10.1039/c7an00648a
  44. Pereira, Patrícia; Queiroz, João A.; Figueiras, Ana; Sousa, Fani. "Current progress on microRNAs-based therapeutics in neurodegenerative diseases". Wiley Interdisciplinary Reviews: RNA 8 3 (2016): e1409. http://dx.doi.org/10.1002/wrna.1409.
    Published • 10.1002/wrna.1409
  45. Pereira, Patrícia; Barreira, Maria; Queiroz, João A.; Veiga, Francisco; Sousa, Fani; Figueiras, Ana. "Smart micelleplexes as a new therapeutic approach for RNA delivery". Expert Opinion on Drug Delivery 14 3 (2016): 353-371. http://dx.doi.org/10.1080/17425247.2016.1214567.
    Published • 10.1080/17425247.2016.1214567
  46. Patrícia Pereira; João A. Queiroz; Ana Figueiras; Fani Sousa. "Affinity approaches in RNAi-based therapeutics purification". Journal of Chromatography B 1021 (2016): 45-56. https://doi.org/10.1016/j.jchromb.2016.01.022.
    10.1016/j.jchromb.2016.01.022
  47. Carvalho, Josué; Ferreira, João; Pereira, P.; Coutinho, E.; Guédin, Aurore; Nottelet, Pierre; Salgado, Gilmar F.; et al. "Stabilization of novel immunoglobulin switch regions G-quadruplexes by naphthalene and quinoline-based ligands". Tetrahedron 72 9 (2016): 1229-1237. http://dx.doi.org/10.1016/j.tet.2016.01.022.
    Published • 10.1016/j.tet.2016.01.022
  48. Pereira, Patrícia; Pedro, Augusto Q.; Tomás, Joana; Maia, Cláudio J.; Queiroz, João A.; Figueiras, Ana; Sousa, Fani. "Advances in time course extracellular production of human pre-miR-29b from Rhodovulum sulfidophilum". Applied Microbiology and Biotechnology 100 8 (2016): 3723-3734. http://dx.doi.org/10.1007/s00253-016-7350-x.
    Published • 10.1007/s00253-016-7350-x
  49. Pereira, Patrícia A.; Tomás, Joana F.; Queiroz, João A.; Figueiras, Ana R.; Sousa, Fani. "Recombinant pre-miR-29b for Alzheimer´s disease therapeutics". Scientific Reports 6 1 (2016): 19946-19946. http://dx.doi.org/10.1038/srep19946.
    Published • 10.1038/srep19946
  50. Diamantino, T.; Pereira, P.; Queiroz, J.A.; Sousa, Â.; Sousa, F.. "Minicircle DNA purification using a CIM® DEAE-1 monolithic support". Journal of Separation Science 39 18 (2016): 3544-3549. http://www.scopus.com/inward/record.url?eid=2-s2.0-84988447206&partnerID=MN8TOARS.
    10.1002/jssc.201600375
  51. Pereira, P.; Cruz, C.; Queiroz, J.A.; Figueiras, A.; Sousa, F.. "Analysis of pre-MIR-29b binding conditions to amino acids by using a surface plasmon resonance biosensor". Analytical Methods 8 1 (2016): 205-213. http://www.scopus.com/inward/record.url?eid=2-s2.0-84952802419&partnerID=MN8TOARS.
    10.1039/c5ay02329g
  52. Bicho, D.; Caramelo-Nunes, C.; Sousa, A.; Sousa, F.; Queiroz, J.A.; Tomaz, C.T.. "Purification of influenza deoxyribonucleic acid-based vaccine using agmatine monolith". Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 1012-1013 (2016): 153-161. http://www.scopus.com/inward/record.url?eid=2-s2.0-84955605658&partnerID=MN8TOARS.
    10.1016/j.jchromb.2015.12.036
  53. Santos, T.; Pereira, P.; Sousa, F.; Queiroz, J.A.; Cruz, C.. "Purification of supercoiled G-quadruplex pDNA for in vitro transcription". Separation and Purification Technology 163 (2016): 59-71. http://www.scopus.com/inward/record.url?eid=2-s2.0-84959323606&partnerID=MN8TOARS.
    10.1016/j.seppur.2016.02.036
  54. Gaspar, Vítor M.; Moreira, André F.; Costa, Elisabete C.; Queiroz, João A.; Sousa, Fani; Pichon, Chantal; Correia, Ilídio J.. "Gas-generating TPGS-PLGA microspheres loaded with nanoparticles (NIMPS) for co-delivery of minicircle DNA and anti-tumoral drugs". Colloids and Surfaces B: Biointerfaces 134 (2015): 287-294. http://dx.doi.org/10.1016/j.colsurfb.2015.07.004.
    10.1016/j.colsurfb.2015.07.004
  55. Salvado, Rúben; Sousa, Fani; Queiroz, João; Costa, Diana. "Development of mitochondrial targeting plasmid DNA nanoparticles: Characterization and in vitro studies". Colloids and Surfaces A: Physicochemical and Engineering Aspects 480 (2015): 287-295. http://dx.doi.org/10.1016/j.colsurfa.2014.12.013.
    Published • 10.1016/j.colsurfa.2014.12.013
  56. Martins, L.M.; Pedro, A.Q.; Oppolzer, D.; Sousa, F.; Queiroz, J.A.; Passarinha, L.A.. "Enhanced biosynthesis of plasmid DNA from Escherichia coli VH33 using Box–Behnken design associated to aromatic amino acids pathway". Biochemical Engineering Journal 98 (2015): 117-126. http://dx.doi.org/10.1016/j.bej.2015.02.001.
    Published • 10.1016/j.bej.2015.02.001
  57. Bicho, D.; Santos, B.F.; Caramelo-Nunes, C.; Sousa, A.; Sousa, F.; Queiroz, J.A.; Tomaz, C.T.. "Application of ethylenediamine monolith to purify a hemagglutinin influenza deoxyribonucleic acid-based vaccine". Separation and Purification Technology 154 (2015): 320-327. http://www.scopus.com/inward/record.url?eid=2-s2.0-84943535397&partnerID=MN8TOARS.
    10.1016/j.seppur.2015.09.046
  58. Almeida, A.M.; Queiroz, J.A.; Sousa, F.; Sousa, A.. "Optimization of supercoiled HPV-16 E6/E7 plasmid DNA purification with arginine monolith using design of experiments". Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 978-979 (2015): 145-150. http://www.scopus.com/inward/record.url?eid=2-s2.0-84919800600&partnerID=MN8TOARS.
    10.1016/j.jchromb.2014.12.004
  59. Amorim, L.F.A.; Sousa, F.; Queiroz, J.A.; Cruz, C.; Sousa, A.. "Screening of l -histidine-based ligands to modify monolithic supports and selectively purify the supercoiled plasmid DNA isoform". Journal of Molecular Recognition 28 6 (2015): 349-358. http://www.scopus.com/inward/record.url?eid=2-s2.0-84928111894&partnerID=MN8TOARS.
    10.1002/jmr.2449
  60. Silva, M.S.; Graça, V.C.; Reis, L.V.; Santos, P.F.; Silvestre, S.; Granadeiro, L.; Almeida, P.; Queiroz, J.A.; Sousa, F.. "3,3'-Diamino-N-methyldipropylamine as a versatile affinity ligand". Journal of Separation Science 38 5 (2015): 732-740. http://www.scopus.com/inward/record.url?eid=2-s2.0-84924169977&partnerID=MN8TOARS.
    10.1002/jssc.201400656
  61. Ferreira, S.; Carvalho, J.; Valente, J.F.A.; Corvo, M.C.; Cabrita, E.J.; Sousa, F.; Queiroz, J.A.; Cruz, C.. "Affinity analysis and application of dipeptides derived from l-tyrosine in plasmid purification". Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 1006 (2015): 47-58. http://www.scopus.com/inward/record.url?eid=2-s2.0-84946410380&partnerID=MN8TOARS.
    10.1016/j.jchromb.2015.10.025
  62. Gaspar, V.M.; Baril, P.; Costa, E.C.; De Melo-Diogo, D.; Foucher, F.; Queiroz, J.A.; Sousa, F.; Pichon, C.; Correia, I.J.. "Bioreducible poly(2-ethyl-2-oxazoline)-PLA-PEI-SS triblock copolymer micelles for co-delivery of DNA minicircles and Doxorubicin". Journal of Controlled Release 213 (2015): 175-191. http://www.scopus.com/inward/record.url?eid=2-s2.0-84937550858&partnerID=MN8TOARS.
    10.1016/j.jconrel.2015.07.011
  63. Gaspar, Vítor; Melo-Diogo, Duarte de; Costa, Elisabete; Moreira, André; Queiroz, João; Pichon, Chantal; Correia, Ilídio; Sousa, Fani. "Minicircle DNA vectors for gene therapy: advances and applications". Expert Opinion on Biological Therapy 15 3 (2014): 353-379. http://dx.doi.org/10.1517/14712598.2015.996544.
    Published • 10.1517/14712598.2015.996544
  64. Aguilar, P.A.; Twarda, A.; Sousa, F.; Dias-Cabral, A.C.. "Thermodynamic study of the interaction between linear plasmid deoxyribonucleic acid and an anion exchange support under linear and overloaded conditions". Journal of Chromatography A 1372 (2014): 166-173. http://dx.doi.org/10.1016/j.chroma.2014.11.002.
    Published • 10.1016/j.chroma.2014.11.002
  65. Mota, E.; Sousa, F.; Queiroz, J. A.; Cruz, C.. "Quantitative analysis of the interaction between L-methionine derivative and oligonucleotides". Journal of Biochemistry 157 4 (2014): 261-270. http://dx.doi.org/10.1093/jb/mvu073.
    Published • 10.1093/jb/mvu073
  66. Gaspar, Vítor M.; Costa, Elisabete C.; Queiroz, João A.; Pichon, Chantal; Sousa, Fani; Correia, Ilídio J.. "Folate-Targeted Multifunctional Amino Acid-Chitosan Nanoparticles for Improved Cancer Therapy". Pharmaceutical Research 32 2 (2014): 562-577. http://dx.doi.org/10.1007/s11095-014-1486-0.
    Published • 10.1007/s11095-014-1486-0
  67. Cruz, Carla; Sousa, Ângela; Mota, Élia; Sousa, Fani; Queiroz, João A.. "Quantitative analysis of histamine- and agmatine–DNA interactions using surface plasmon resonance". International Journal of Biological Macromolecules 70 (2014): 131-137. http://dx.doi.org/10.1016/j.ijbiomac.2014.06.044.
    Published • 10.1016/j.ijbiomac.2014.06.044
  68. Gaspar, Vítor M.; Maia, Cláudio J.; Queiroz, João A.; Pichon, Chantal; Correia, Ilídio J.; Sousa, Fani. "Improved Minicircle DNA Biosynthesis for Gene Therapy Applications". Human Gene Therapy Methods 25 2 (2014): 93-105. http://dx.doi.org/10.1089/hgtb.2013.020.
    10.1089/hgtb.2013.020
  69. Nunes, C.; Sousa, A.; Nunes, J.C.; Morão, A.M.; Sousa, F.; Queiroz, J.A.. "Supercoiled plasmid DNA purification by integrating membrane technology with a monolithic chromatography". Journal of Separation Science 37 11 (2014): 1229-1236. http://www.scopus.com/inward/record.url?eid=2-s2.0-84902117888&partnerID=MN8TOARS.
    10.1002/jssc.201301160
  70. Bicho, D.; Sousa, A.; Sousa, F.; Queiroz, J.; Tomaz, C.. "Effect of chromatographic conditions and plasmid DNA size on the dynamic binding capacity of a monolithic support". Journal of Separation Science 37 17 (2014): 2284-2292. http://www.scopus.com/inward/record.url?eid=2-s2.0-84906848855&partnerID=MN8TOARS.
    10.1002/jssc.201400127
  71. Santos, J.; Sousa, F.; Queiroz, J.; Costa, D.. "Rhodamine based plasmid DNA nanoparticles for mitochondrial gene therapy". Colloids and Surfaces B: Biointerfaces 121 (2014): 129-140. http://www.scopus.com/inward/record.url?eid=2-s2.0-84902972220&partnerID=MN8TOARS.
    10.1016/j.colsurfb.2014.06.003
  72. Pereira, P.; Sousa, T.; Queiroz, J.A.; Figueiras, A.; Sousa, F.. "Pharmaceutical-grade pre-miR-29 purification using an agmatine monolithic support". Journal of Chromatography A 1368 (2014): 173-182. http://www.scopus.com/inward/record.url?eid=2-s2.0-84908209237&partnerID=MN8TOARS.
    10.1016/j.chroma.2014.09.080
  73. Sousa, A.; Almeida, A.M.; Cernigoj, U.; Sousa, F.; Queiroz, J.A.. "Histamine monolith versatility to purify supercoiled plasmid deoxyribonucleic acid from Escherichia coli lysate". Journal of Chromatography A 1355 (2014): 125-133. http://www.scopus.com/inward/record.url?eid=2-s2.0-84904286739&partnerID=MN8TOARS.
    10.1016/j.chroma.2014.06.003
  74. Pereira, P.; Sousa, T.; Queiroz, J.; Figueiras, A.; Sousa, F.. "New approach for purification of pre-miR-29 using lysine-affinity chromatography". Journal of Chromatography A 1331 (2014): 129-132. http://www.scopus.com/inward/record.url?eid=2-s2.0-84896715699&partnerID=MN8TOARS.
    10.1016/j.chroma.2014.01.033
  75. Graça, V.C.; Silva, M.S.; Reis, L.V.; Sousa, F.; Almeida, P.; Queiroz, J.A.; Santos, P.F.. "Ethylenediamine-Derived Chromatographic Ligand to Separate BSA, Lysozyme, and RNase A". Chromatographia 77 21-22 (2014): 1529-1537. http://www.scopus.com/inward/record.url?eid=2-s2.0-84911950967&partnerID=MN8TOARS.
    10.1007/s10337-014-2749-y
  76. Sousa, T.; Pereira, P.; Sousa, F.; Queiroz, J.A.. "Binding mechanisms for histamine and agmatine ligands in plasmid deoxyribonucleic acid purifications". Journal of Chromatography A 1366 (2014): 110-119. http://www.scopus.com/inward/record.url?eid=2-s2.0-84907969355&partnerID=MN8TOARS.
    10.1016/j.chroma.2014.09.031
  77. Valente, J.F.A.; Sousa, A.; Queiroz, J.A.; Sousa, F.. "Selective purification of supercoiled p53-encoding pDNA with l-methionine-agarose matrix". Analytical Biochemistry 459 (2014): 61-69. http://www.scopus.com/inward/record.url?eid=2-s2.0-84902657783&partnerID=MN8TOARS.
    10.1016/j.ab.2014.05.011
  78. Martins, R.; Queiroz, J.A.; Sousa, F.. "Ribonucleic acid purification". Journal of Chromatography A 1355 (2014): 1-14. http://www.scopus.com/inward/record.url?eid=2-s2.0-84904302278&partnerID=MN8TOARS.
    10.1016/j.chroma.2014.05.075
  79. Alves, L.P.; Ramos, S.S.; Sousa, F.; Boto, R.E.F.; Almeida, P.. "A Benzothiazolium Salt as Chromatography Ligand for Protein Purification". Chromatographia 77 23-24 (2014): 1597-1605. http://www.scopus.com/inward/record.url?eid=2-s2.0-84911999848&partnerID=MN8TOARS.
    10.1007/s10337-014-2774-x
  80. Pereira, P.; Sousa, Â.; Queiroz, J.; Correia, I.; Figueiras, A.; Sousa, F.. "Purification of pre-miR-29 by arginine-affinity chromatography". Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 951-952 1 (2014): 16-23. http://www.scopus.com/inward/record.url?eid=2-s2.0-84893705000&partnerID=MN8TOARS.
    10.1016/j.jchromb.2014.01.020
  81. Mota, E.; Sousa, F.; Sousa, Â.; Queiroz, J.A.; Cruz, C.. "Molecular recognition of oligonucleotides and plasmid DNA by L-methionine". Journal of Molecular Recognition 27 10 (2014): 588-596. http://www.scopus.com/inward/record.url?eid=2-s2.0-84906888435&partnerID=MN8TOARS.
    10.1002/jmr.2382
  82. Afonso, A.; Pereira, P.; Queiroz, J.A.; Sousa, T.; Sousa, F.. "Purification of pre-miR-29 by a new O-phospho-l-tyrosine affinity chromatographic strategy optimized using design of experiments". Journal of Chromatography A 1343 (2014): 119-127. http://www.scopus.com/inward/record.url?eid=2-s2.0-84899989051&partnerID=MN8TOARS.
    10.1016/j.chroma.2014.03.071
  83. Gaspar, V.M.; Gonçalves, C.; De Melo-Diogo, D.; Costa, E.C.; Queiroz, J.A.; Pichon, C.; Sousa, F.; Correia, I.J.. "Poly(2-ethyl-2-oxazoline)-PLA-g-PEI amphiphilic triblock micelles for co-delivery of minicircle DNA and chemotherapeutics". Journal of Controlled Release 189 (2014): 90-104. http://www.scopus.com/inward/record.url?eid=2-s2.0-84904182939&partnerID=MN8TOARS.
    10.1016/j.jconrel.2014.06.040
  84. Martins, R.; Queiroz, J. A.; Sousa, F.. "New approach in RNA quantification using arginine-affinity chromatography: potential application in eukaryotic and chemically synthesized RNA". Analytical and Bioanalytical Chemistry 405 27 (2013): 8849-8858. http://dx.doi.org/10.1007/s00216-013-7334-y.
    Published • 10.1007/s00216-013-7334-y
  85. Gaspar, Vítor M.; Cruz, Carla; Queiroz, João A.; Pichon, Chantal; Correia, Ilídio J.; Sousa, Fani. "Sensitive Detection of Peptide–Minicircle DNA Interactions by Surface Plasmon Resonance". Analytical Chemistry 85 4 (2013): 2304-2311. http://dx.doi.org/10.1021/ac303288x.
    Published • 10.1021/ac303288x
  86. Gonçalves, A.M.; Pedro, A.Q.; Maia, C.; Sousa, F.; Queiroz, J.A.; Passarinha, L.A.. "Pichia pastoris: A recombinant microfactory for antibodies and human membrane proteins". Journal of Microbiology and Biotechnology 23 5 (2013): 587-601. http://www.scopus.com/inward/record.url?eid=2-s2.0-84877343772&partnerID=MN8TOARS.
    10.4014/jmb.1210.10063
  87. Gaspar, V.M.; Marques, J.G.; Sousa, F.; Louro, R.O.; Queiroz, J.A.; Correia, I.J.. "Biofunctionalized nanoparticles with pH-responsive and cell penetrating blocks for gene delivery". Nanotechnology 24 27 (2013): http://www.scopus.com/inward/record.url?eid=2-s2.0-84879292912&partnerID=MN8TOARS.
    10.1088/0957-4484/24/27/275101
  88. Soares, A.; Queiroz, J.A.; Sousa, F.; Sousa, A.. "Purification of human papillomavirus 16 E6/E7 plasmid deoxyribonucleic acid-based vaccine using an arginine modified monolithic support". Journal of Chromatography A 1320 (2013): 72-79. http://www.scopus.com/inward/record.url?eid=2-s2.0-84887611735&partnerID=MN8TOARS.
    10.1016/j.chroma.2013.10.053
  89. Silva, M.S.; Graça, V.C.; Reis, L.V.; Santos, P.F.; Almeida, P.; Queiroz, J.A.; Sousa, F.. "Protein purification by aminosquarylium cyanine dye-affinity chromatography". Biomedical Chromatography 27 12 (2013): 1671-1679. http://www.scopus.com/inward/record.url?eid=2-s2.0-84887190801&partnerID=MN8TOARS.
    10.1002/bmc.2978
  90. Cruz, C.; Sousa, A.; Sousa, F.; Queiroz, J.A.. "Affinity analysis between immobilized l-arginine and plasmid isoforms provided by surface plasmon resonance". Analytical Methods 5 7 (2013): 1682-1686. http://www.scopus.com/inward/record.url?eid=2-s2.0-84879509327&partnerID=MN8TOARS.
    10.1039/c3ay40213d
  91. Mota, É.; Sousa, Â.; Cernigoj, U.; Queiroz, J.A.; Tomaz, C.T.; Sousa, F.. "Rapid quantification of supercoiled plasmid deoxyribonucleic acid using a monolithic ion exchanger". Journal of Chromatography A 1291 (2013): 114-121. http://www.scopus.com/inward/record.url?eid=2-s2.0-84876791056&partnerID=MN8TOARS.
    10.1016/j.chroma.2013.03.070
  92. Pereira, Patrícia; Jorge, Andreia F.; Martins, Rita; Pais, Alberto A.C.C.; Sousa, Fani; Figueiras, Ana. "Characterization of polyplexes involving small RNA". Journal of Colloid and Interface Science 387 1 (2012): 84-94. http://dx.doi.org/10.1016/j.jcis.2012.07.088.
    Published • 10.1016/j.jcis.2012.07.088
  93. Sousa, Â.; Sousa, F.; Queiroz, J.A.. "Advances in chromatographic supports for pharmaceutical-grade plasmid DNA purification". Journal of Separation Science 35 22 (2012): 3046-3058. http://www.scopus.com/inward/record.url?eid=2-s2.0-84870040472&partnerID=MN8TOARS.
    10.1002/jssc.201200307
  94. Martins, R.; Maia, C.J.; Queiroz, J.A.; Sousa, F.. "A new strategy for RNA isolation from eukaryotic cells using arginine affinity chromatography". Journal of Separation Science 35 22 (2012): 3217-3226. http://www.scopus.com/inward/record.url?eid=2-s2.0-84870045405&partnerID=MN8TOARS.
    10.1002/jssc.201200338
  95. Cruz, C.; Sousa, A.; Sousa, F.; Queiroz, J.A.. "Study of the specific interaction between l-methionine chromatography support and nucleotides". Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 909 (2012): 1-5. http://www.scopus.com/inward/record.url?eid=2-s2.0-84868249738&partnerID=MN8TOARS.
    10.1016/j.jchromb.2012.09.037
  96. Martins, R.; Queiroz, J.A.; Sousa, F.. "Histidine affinity chromatography-based methodology for the simultaneous isolation of Escherichia coli small and ribosomal RNA". Biomedical Chromatography 26 7 (2012): 781-788. http://www.scopus.com/inward/record.url?eid=2-s2.0-84862202716&partnerID=MN8TOARS.
    10.1002/bmc.1729
  97. Gaspar, Vítor M.; Correia, Ilídio J.; Sousa, Ângela; Silva, Filomena; Paquete, Catarina M.; Queiroz, João A.; Sousa, Fani. "Nanoparticle mediated delivery of pure P53 supercoiled plasmid DNA for gene therapy". Journal of Controlled Release 156 2 (2011): 212-222. http://dx.doi.org/10.1016/j.jconrel.2011.08.007.
    10.1016/j.jconrel.2011.08.007
  98. Sousa, F.; Queiroz, J.A.. "Supercoiled plasmid quality assessment by analytical arginine-affinity chromatography". Journal of Chromatography A 1218 1 (2011): 124-129. http://www.scopus.com/inward/record.url?eid=2-s2.0-78650417249&partnerID=MN8TOARS.
    10.1016/j.chroma.2010.11.002
  99. Morão, A.M.; Nunes, J.C.; Sousa, F.; Pessoa de Amorim, M.T.; Escobar, I.C.; Queiroz, J.A.. "Ultrafiltration of supercoiled plasmid DNA: Modeling and application". Journal of Membrane Science 378 1-2 (2011): 280-289. http://www.scopus.com/inward/record.url?eid=2-s2.0-79959984677&partnerID=MN8TOARS.
    10.1016/j.memsci.2011.05.017
  100. Sousa, A.; Sousa, F.; Queiroz, J.A.. "Impact of lysine-affinity chromatography on supercoiled plasmid DNA purification". Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 879 30 (2011): 3507-3515. http://www.scopus.com/inward/record.url?eid=2-s2.0-80555149385&partnerID=MN8TOARS.
    10.1016/j.jchromb.2011.09.032
  101. Sousa, A.; Bicho, D.; Tomaz, C.T.; Sousa, F.; Queiroz, J.A.. "Performance of a non-grafted monolithic support for purification of supercoiled plasmid DNA". Journal of Chromatography A 1218 13 (2011): 1701-1706. http://www.scopus.com/inward/record.url?eid=2-s2.0-79952363162&partnerID=MN8TOARS.
    10.1016/j.chroma.2010.12.018
  102. Sousa, A.; Tomaz, C.T.; Sousa, F.; Queiroz, J.A.. "Successful application of monolithic innovative technology using a carbonyldiimidazole disk to purify supercoiled plasmid DNA suitable for pharmaceutical applications". Journal of Chromatography A 1218 46 (2011): 8333-8343. http://www.scopus.com/inward/record.url?eid=2-s2.0-80054865157&partnerID=MN8TOARS.
    10.1016/j.chroma.2011.09.033
  103. Gaspar, V M; Sousa, F; Queiroz, J A; Correia, I J. "Formulation of chitosan–TPP–pDNA nanocapsules for gene therapy applications". Nanotechnology 22 1 (2010): 015101. http://dx.doi.org/10.1088/0957-4484/22/1/015101.
    10.1088/0957-4484/22/1/015101
  104. Martins, R.; Queiroz, J.A.; Sousa, F.. "A new affinity approach to isolate Escherichia coli 6S RNA with histidine-chromatography". Journal of Molecular Recognition 23 6 (2010): 519-524. http://www.scopus.com/inward/record.url?eid=2-s2.0-78049417545&partnerID=MN8TOARS.
    10.1002/jmr.1078
  105. Sousa, F.; Cruz, C.; Queiroz, J.A.. "Amino acids-nucleotides biomolecular recognition: From biological occurrence to affinity chromatography". Journal of Molecular Recognition 23 6 (2010): 505-518. http://www.scopus.com/inward/record.url?eid=2-s2.0-78049440483&partnerID=MN8TOARS.
    10.1002/jmr.1053
  106. Sousa, A.; Sousa, F.; Queiroz, J.A.. "Differential interactions of plasmid DNA, RNA and genomic DNA with amino acid-based affinity matrices". Journal of Separation Science 33 17-18 (2010): 2610-2618. http://www.scopus.com/inward/record.url?eid=2-s2.0-77956918746&partnerID=MN8TOARS.
    10.1002/jssc.201000347
  107. Sousa, Fani; Prazeres, Duarte M. F.; Queiroz, João A.. "Improvement of transfection efficiency by using supercoiled plasmid DNA purified with arginine affinity chromatography". The Journal of Gene Medicine 11 1 (2009): 79-88. http://dx.doi.org/10.1002/jgm.1272.
    Published • 10.1002/jgm.1272
  108. Sousa, F.; Passarinha, L.; Queiroz, J.A.. "Biomedical application of plasmid DNA in gene therapy: A new challenge for chromatography". Biotechnology and Genetic Engineering Reviews 26 1 (2009): 83-116. http://www.scopus.com/inward/record.url?eid=2-s2.0-77749249237&partnerID=MN8TOARS.
    Published • 10.5661/bger-26-83
  109. Sousa, Â.; Sousa, F.; Queiroz, J.A.. "Selectivity of arginine chromatography in promoting different interactions using synthetic oligonucleotides as model". Journal of Separation Science 32 10 (2009): 1665-1672. http://www.scopus.com/inward/record.url?eid=2-s2.0-67650001336&partnerID=MN8TOARS.
    10.1002/jssc.200800690
  110. Sousa, F.; Prazeres, D.M.F.; Queiroz, J.A.. "Binding and elution strategy for improved performance of arginine affinity chromatography in supercoiled plasmid DNA purification". Biomedical Chromatography 23 2 (2009): 160-165. http://www.scopus.com/inward/record.url?eid=2-s2.0-61349098946&partnerID=MN8TOARS.
    10.1002/bmc.1097
  111. Sousa, A.; Sousa, F.; Prazeres, D.M.F.; Queiroz, J.A.. "Histidine affinity chromatography of homo-oligonucleotides. Role of multiple interactions on retention". Biomedical Chromatography 23 7 (2009): 745-753. http://www.scopus.com/inward/record.url?eid=2-s2.0-69749128313&partnerID=MN8TOARS.
    10.1002/bmc.1179
  112. Boto, R.E.F.; Anyanwu, U.; Sousa, F.; Almeida, P.; Queiroz, J.A.. "Thiacarbocyanine as ligand in dye-affinity chromatography for protein purification. II. Dynamic binding capacity using lysozyme as a model". Biomedical Chromatography 23 9 (2009): 987-993. http://www.scopus.com/inward/record.url?eid=2-s2.0-70349478902&partnerID=MN8TOARS.
    10.1002/bmc.1212
  113. Morão, A.; Nunes, J.C.; Sousa, F.; Amorim, M.T.P.d.; Escobar, I.C.; Queiroz, J.A.. "Development of a model for membrane filtration of long and flexible macromolecules: Application to predict dextran and linear DNA rejections in ultrafiltration". Journal of Membrane Science 336 1-2 (2009): 61-70. http://www.scopus.com/inward/record.url?eid=2-s2.0-67349194357&partnerID=MN8TOARS.
    10.1016/j.memsci.2009.03.007
  114. Silva, F.; Passarinha, L.; Sousa, F.; Queiroz, J.A.; Domingues, F.C.. "Influence of growth conditions on plasmid DNA production". Journal of Microbiology and Biotechnology 19 11 (2009): 1408-1414. http://www.scopus.com/inward/record.url?eid=2-s2.0-71549136850&partnerID=MN8TOARS.
    10.4014/jmb.0805.0329
  115. Sousa, A.; Sousa, F.; Queiroz, J.A.. "Biorecognition of supercoiled plasmid DNA isoform in lysine-affinity chromatography". Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 877 27 (2009): 3257-3260. http://www.scopus.com/inward/record.url?eid=2-s2.0-69749124060&partnerID=MN8TOARS.
    10.1016/j.jchromb.2009.08.021
  116. Sousa, Fani; Prazeres, Duarte M.F.; Queiroz, João A.. "Affinity chromatography approaches to overcome the challenges of purifying plasmid DNA". Trends in Biotechnology 26 9 (2008): 518-525. http://dx.doi.org/10.1016/j.tibtech.2008.05.005.
    Published • 10.1016/j.tibtech.2008.05.005
  117. Sousa, F.; Matos, T.; Prazeres, D.M.F.; Queiroz, J.A.. "Specific recognition of supercoiled plasmid DNA in arginine affinity chromatography". Analytical Biochemistry 374 2 (2008): 432-434. http://www.scopus.com/inward/record.url?eid=2-s2.0-39149129979&partnerID=MN8TOARS.
    10.1016/j.ab.2007.11.005
  118. Sousa, F.; Prazeres, D.M.F.; Queiroz, J.A.. "Circular dichroism investigation of the effect of plasmid DNA structure on retention in histidine chromatography". Archives of Biochemistry and Biophysics 467 2 (2007): 154-162. http://dx.doi.org/10.1016/j.abb.2007.08.027.
    Published • 10.1016/j.abb.2007.08.027
  119. Sousa, F.; Prazeres, D.M.F.; Queiroz, J.A.. "Dynamic binding capacity of plasmid DNA in histidine-agarose chromatography". Biomedical Chromatography 21 9 (2007): 993-998. http://www.scopus.com/inward/record.url?eid=2-s2.0-34547966151&partnerID=MN8TOARS.
    10.1002/bmc.846
  120. Sousa, F.; Ferreira, S.; Queiroz, J.; Domingues, F.. "Production of a fusion protein containing the antigenic domain 1 of human cytomegalovirus glycoprotein B". Journal of Microbiology and Biotechnology 16 7 (2006): 1026-1031. http://www.scopus.com/inward/record.url?eid=2-s2.0-33746894082&partnerID=MN8TOARS.
    Published
  121. Sousa, F.; Freitas, S.; Azzoni, A.R.; Prazeres, D.M.F.; Queiroz, J.; Freitas, Sindélia; Sousa, Fani; et al. "Selective purification of supercoiled plasmid DNA from clarified cell lysates with a single histidine-agarose chromatography step". Biotechnology and Applied Biochemistry 45 3 (2006): 131-140. http://www.scopus.com/inward/record.url?eid=2-s2.0-33750607427&partnerID=MN8TOARS.
    Published • 10.1042/BA20060082
  122. Sousa, F.; Melo, A.; Almeida, S.; Paixão, P.; Queiroz, J.A.; Domingues, F.C.. "Isolation of a fusion protein containing the antigenic domain 1 of human cytomegalovirus glycoprotein B and its application in ELISA tests". Biotechnology Letters 28 2 (2006): 73-77. http://www.scopus.com/inward/record.url?eid=2-s2.0-29244483677&partnerID=MN8TOARS.
    10.1007/s10529-005-4949-x
  123. Sousa, F.; Tomaz, C.T.; Prazeres, D.M.F.; Queiroz, J.A.. "Separation of supercoiled and open circular plasmid DNA isoforms by chromatography with a histidine-agarose support". Analytical Biochemistry 343 1 (2005): 183-185. http://www.scopus.com/inward/record.url?eid=2-s2.0-22144443546&partnerID=MN8TOARS.
    10.1016/j.ab.2005.03.011
  124. Ferreira, S.; Sousa, F.; Queiroz, J.A.; Domingues, F.C.. "Improved recovery of a fusion protein containing the antigenic domain 1 of the human cytomegalovirus glycoprotein B". Biotechnology Letters 27 16 (2005): 1241-1245. http://www.scopus.com/inward/record.url?eid=2-s2.0-24744466678&partnerID=MN8TOARS.
    10.1007/s10529-005-0024-x

Intellectual property

Patent
  1. Freire MG; Sousa, F; Queiroz JA; Neves M; Martins JC; Pereira P; Pedro AQ. 2019. "Separação de ácidos nucleicos utilizando líquidos iónicos como ligandos cromatográficos multimodais em matrizes macroporosas".
    Granted/Issued
Provisional application for patent
  1. 2019. "Chromatographic Matrix, Method and Uses Thereof".
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2023 RNA biopharmaceuticals: from recombinant production to the cellular application: The case study of miRNAs for Alzheimer’s disease 2nd Symposium on Alzheimer’s disease
2022 Amino acids as a powerful tool for DNA and RNA purification PATh/IL2BioPro/mVACCIL Workshop
2022 Challenges on DNA and RNA purification: Can amino acids help?. Thursday Talks at Merck KGaA
2021 RNA as biopharmaceuticals – the challenges behind bioprocessing MicroBiotec
2019/05/13 The effect of the ionic liquid cation on the DNA stability and purification using aqueous biphasic systems ISGC - The International Symposium on Green Chemistry 2019
2018/09/09 Pri-miR-375 expressing mcDNA vector: assessing the role in HPV oncoproteins silencing ESBES - 12th Symposium of the European Society of Biochemical Engineering Sciences
(Lisboa, Portugal)
2018/06/15 Sample displacement chromatography – old principle for modern applications 8th Monolith Summer School Symposium (MSS)
(Portoroz, Slovenia)
2018 Nucleolin as target of RNA G-quadruplex aptamer for prostate cancer diagnosis 6th National Meeting of Chemistry Students
(Covilhã, Portugal)
2017/11/22 Development and optimization of new monolithic adsorbents for plasmid DNA purification by affinity chromatography ABC2 - Anything But Conventional Chromatography
(Lisboa, Portugal)
2017/09/24 Bioprocessing of Interferon alpha 2b using ionic-liquid-based systems BPP - Biopartitioning and Purification Conference 2017
(Copenhaga, Denmark)
2017/05/17 Small RNA stabilization using biocompatible cholinium-based ionic liquids II International Congress in Health Sciences Research: Towards Innovation and entrepreneurship
(Covilhã, Portugal)
2017/05/17 A library of amino acids to screen adsorbents suitable for sc p53 encoding plasmid isolation II International Congress in Health Sciences Research: Towards Innovation and entrepreneurship
(Covlhã, Portugal)
2017 Biopharmaceuticals purification based on amino acids-nucleic acids interactions MicroBiotec
(Porto, Portugal)
2017 Novel alternatives for interferon alpha2b purification: chromatography vs ionic liquid aqueous biphasic systems II International Congress in Health Sciences Research: Towards Innovation and Entrepreneurship
(Covilhã, Portugal)
2017 Affinity chromatography for Minicircle DNA purification: lysine- and cadaverine-monoliths performance II International Congress in Health Sciences Research: Towards Innovation and Entrepreneurship - Trends in Biotechnology and Biomedical Applications
(Covilhã, Portugal)
2016/11 Innovative diagnosis of prostate cancer based on pre-miRNA G-quadruplexes 3 minutes, 1 slide, your Thesis…!
(Portugal)
2016 Monoliths for purification of nucleic acids: The study of the cases of pDNA, mcDNA and RNA. 7th Monolith Summer School & Symposium 2016
(Portoroz, Slovenia)
2016 Versatility of arginine monolithic support in the purification of therapeutic DNA vectors XVI Congresso Latino-Americano de Cromatografia (COLACRO) e 9º Encontro Nacional de Cromatografia
(Lisboa, Portugal)
2016 Superporous affinity chromatography for the supercoiled p53 encoding plasmid isolation ISPPP - International Symposium on the Separation of Proteins, Peptides and Polynucleotides
(Salzburg, Austria)
2016 p53 supercoiled encoding plasmid in different cancer cell lines – Transfection and Expression effects Bio.Iberoamérica (2016)
(Salamanca, Spain)
2015/02/11 Simultaneous Delivery of Drugs and Genes by Multi-block Polymeric Nanomicelles for Synergistic Cancer Therapy Nanoscience and Nanotechnology International Conference – NanoPT
(Porto, Portugal)
2015 Supercoiled HPV16 E6/E7 plasmid DNA-based vaccine purified by affinity chromatography for in vitro transfection studies VIII Jornadas sobre Tecnologia e Saúde, Instituto Politécnico da Guarda
(Guarda, Portugal)
2015 Amino acid derivatized monoliths for purification of a DNA vaccine against influenza 4th Portuguese BioEngineering Meeting
(Porto, Portugal)
2015 Screening of L-histidine based ligands to purify the supercoiled plasmid DNA isoform 4th Portuguese BioEngineering Meeting
(Porto, Portugal)
2015 Purification of a DNA vaccine against Human Papillomavirus infection using L-histidine and 1-benzyl-L-histidine monoliths VIII Jornadas sobre Tecnologia e Saúde, Instituto Politécnico da Guarda
(GUarda)
2015 DNA vaccine development for HPV treatment: biotechnological production, purification and in vitro application MicroBiotech'15 - Congress of Microbiology and Biotechnology
(Évora, Portugal)
2015 Supercoiled HPV16 E6/E7 plasmid DNA-based vaccine purified by affinity chromatography for in vitro transfection studies VIII Jornadas sobre Tecnologia e Saúde
(GUarda, Portugal)
2014/05/22 Development of Nanosystems for Delivery of Bioactive Molecules with Application in Cancer Therapy Clinical Sessions of the Hospital of Cova da Beira (HCB)
(Covilhã, Portugal)
2014/03/13 Nanomaterials for Delivery of Biomolecules with Application in Cancer Therapy V National Conference of Biomedical Sciences. University of Beira Interior (UBI)
(Covilhã, Portugal)
2014 Agmatine as a ligand for chromatographic purification of supercoiled plasmid DNA-based vaccine against Influenza ESBES-IFIBiop
(Lille, France)
2014 A new chromatographic approach using agmatine as ligand for the separation of pDNA isoforms Monolith Summer School Symposium 2014
(Portoroz, Slovenia)
2014 Screening of histidine-based ligands to modify monolithic supports and purify the supercoiled isoform of different plasmids. Monolith Summer School Symposium 2014
(Portoroz, Slovenia)
2014 Modification of monolithic columns by immobilization of amino acids and derivatives to purify the plasmid DNA vaccine against HPV- 16 6th Monolith Summer School & Symposium 2014
(Portoroz, Slovenia)
2013 Delivery of DNA Biopharmaceuticals by Non- Viral Nanoparticulated Carriers Micro Biotech’13 - Portuguese Congress of Microbiology and Biotechnology
(Aveiro, Portugal)
2013 Purification of Human Papillomavirus16 E6/E7 plasmid DNA-based vaccine using an arginine modified monolithic support Micro Biotech’13 - Portuguese Congress of Microbiology and Biotechnology
(Aveiro, Portugal)
2013 Molecular recognition of polynucleotides and plasmid DNA by L-methionine support 8º Encontro Nacional de Cromatografia
(Covilhã)
2013 Pre-miR-29 purification by amino acids-affinity chromatography 8º Encontro Nacional de Cromatografia
(Covilhã, Portugal)
2013 Affinity-based method for RNA purification pursuing mRNA vaccination New Achievements In Chromatography: 19th International Symposium on Separation Sciences – New achievements in Chromatography
(Porec, Croatia)
2013 Study of the interactions between immobilized L-methionine and oligonucleotides by chromatography, NMR and SPR analysis 19th International Symposium on Separation Sciences – New achievements in Chromatography
(Porec, Croatia)
2012 Development of pDNA purification process using single monolithic chromatographic column SBCN2012 - Bioseparation & Characterization
(Bordeux)
2012 A new monolithic strategy for pharmaceutical-grade plasmid DNA purification 7º Encontro Nacional de Cromatografia
(Porto, Portugal)
2011 Plasmid DNA for Gene Therapy Applications: From the Purification to the In vitro Evaluation MicroBiotec
(Braga, Portugal)
2011 A New Effective Method for Purifying Escherichia coli Small and Ribosomal RNA using Histidine Affinity Chromatography Affinity 2011
(Tavira, Portugal)
2010 Performance of a new methacrylate monolith for supercoiled plasmid DNA dynamic adsorption 12th SBCN 2010
(Lion, France)
2010 Supercoiled plasmid quality assessment by arginine-affinity chromatography – an analytical approach ESBES + ISPPP + ISB Symposia 2010
(Bolonha, Italy)
2009 Plasmid DNA purification for gene therapy applications using a new affinity concept based on amino acid-nucleic acids interaction – An Overview Affinity 2009
(Reykjavik, Iceland)
2009 New lysine-affinity chromatography for biorecognition of supercoiled plasmid DNA isoform Affinity 2009
(Reykjavik, Iceland)
2009 Arginine-affinity chromatography: selectivity for oligonucleotides biorecognition BPP – International Conference on Biopartitioning and Purification
(Uxbridge, United Kingdom)
2008 Temperature-induced conformational changes of pDNA and their influence on histidine-agarose retention – a circular dichroism study ESBES 7 – 7th European Symposium on Biochemical Engineering Science
(Faro, Portugal)
2007 The use of amino acid-based ligands in the chromatographic purification of plasmid DNA 5th HIC/RPC
(Interlaken, Switzerland)
2007 Arginine as a ligand for chromatographic purification of plasmid DNA BPP – International Conference on Biopartitioning and Purification
(Lisboa, Portugal)
2007 The use of amino acid-based ligands in the chromatographic purification of plasmid DNA 5th HIC/RPC
(Interlaken, Switzerland)
2006 Selective purification of supercoiled plasmid DNA with a histidine column CBioSep-WGDSP Meeting
(Lund, Sweden)
2005 Separation of supercoiled and open circular plasmid DNA isoforms by chromatography with a histidine-agarose support 12th European Congress on Biotechnology
(Copenhaga, Denmark)

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2023/12/01 - Current Hybrid p53-mRNA nanoparticles: A promising strategy for lung cancer treatment
Supervisor
2023/12/01 - Current New affinity approaches for mRNA purification
Supervisor
2023 - Current Design of Molecular Beacons to assess recombinant pre-miRNA hairpin structure
Supervisor
2023 - Current The role of miR-9 and miR-29 in Alzheimer’s disease
Supervisor
2023 - Current Oligómeros no reconhecimento específico e purificação de DNA e RNA
Supervisor
2023 - Current Materiais de carbono na remoção de endotoxinas de amostras com aplicação biológica
Supervisor
2023 - Current Development of pH-responsive nanoparticles for the protection and delivery of RNAs to cancer cells
Supervisor
2022/12/01 - Current Magnetic fishing and delivery of pre-miRNA-carbon nanotubes hybrids
Supervisor
2022/10 - Current Ionic liquids as the next generation of excipients for DNA stabilization
Co-supervisor
2021/01 - Current Innovative continuous mode purification of pre-miR-29b biopharmaceuticals from Rhodovulum sulfidophilum extract
Supervisor of Ana Rita Carapito
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2021 - Current Cellular processing of recombinant pre-miRNAs and their role in the pathological hallmarks of Alzheimer's disease
Supervisor of Micaela Riscado
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2021 - Current Purification of p53-minicircle DNA biopharmaceutical using integrated processes based on aqueous biphasic systems and supported ionic liquids
Co-supervisor of Ana Isabel Valente
Universidade de Aveiro, Portugal
2022 - 2023 Molecular docking tools for the screening of affinity ligands for pre-miRNA-29b purification
Supervisor
2022 - 2023 Production of chromatographic supports by 3D printing
Co-supervisor
2021 - 2023 PolyThymine cryogels as a novel approach to the purification of mRNA encoding anti-ADAM17 antibodies
Co-supervisor
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2021 - 2023 Avaliação in vitro da entrega do microRNA-29 em células de Cancro do Pulmão
Co-supervisor
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2021 - 2022 Carbon Nanotubes in RNA capture – characterization and application in biotechnological processes
Supervisor
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2021 - 2022 Purification of pre-miRNA-29 with ionic liquids-based supports
Supervisor
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2020 - 2021/03/12 Design of specific ligands for mRNA stabilization
Supervisor of Sofia Oliveira
 Biotecnologia (Master)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2020 - 2021/03/11 Carbon Nanotubes for nucleic acids adsorption
Supervisor of Pedro Ferreira
 Biotecnologia (Master)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2020 - 2021 Design of Deep Eutectic Solvents to improve RNA stabilization and storage
Supervisor of Ana Ferro
 Biotecnologia (Master)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2018 - 2020 Evaluation of the role of miR-9 and miR-29 in amyloid pathway of Alzheimer’s disease
Supervisor of Micaela Riscado
 Bioquímica (Master)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2016 - 2020 Development of ionic-liquid-based supported materials for the purification of Ribonucleic Acid (RNA). (SFRH/BD/130958/2017)
Co-supervisor
 Engenharia Química – Ramo de Bioengenharia (PhD)
Universidade de Aveiro, Portugal

Universidade da Beira Interior, Portugal
2015 - 2020 Design, production and purification of minicircle DNA encoding pri-miR-375 for treatment of Human Papillomavirus infection (SFRH/BD/102284/2014)
Co-supervisor
 Bioquímica (PhD)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2018 - 2019 Ionic liquids for the purification and stabilization of nucleic acids
Supervisor
 Biotecnologia (Master)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2018 - 2019 Avaliação da Ação de um Vetor Multigénico de DNA Minicircular em Células Cancerígenas do Colo do Útero
Co-supervisor
 Ciências Biomédicas (Master)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2018 - 2019 Desenvolvimento de uma formulação para administração de sRNA
Supervisor
 Ciências Farmacêuticas (Master)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2015 - 2019 Effect of the purification conditions on pDNA activity in cancer therapy (CsF/CNPq/203482/2014-0)
Co-supervisor
 Bioquímica (PhD)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2017 - 2018 Potencial terapêutico de miR-9 e miR-29 na doença de Alzheimer
Supervisor of Bruno Baptista
 Ciências Biomédicas (Master)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2017 - 2018 Novas estratégias de purificação de microRNAs usando líquidos iónicos
Supervisor
 Ciências Biomédicas (Master)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2017 - 2018 DNA minicircular multigénico com potencial ação terapêutica no cancro do colo do útero
Co-supervisor
 Ciências Biomédicas (Master)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2017 - 2018 Biosynthesis of small noncoding RNAs in Rhodovulum sulfidophilum: optimization of the fermentation process
Co-supervisor
 Ciências Biomédicas (Master)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2017 - 2018 Medicamentos Biossimilares: Uma perspetiva atual
Supervisor
 Ciências Farmacêuticas (Master)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2015 - 2018 Desenvolvimento e avaliação de adsorventes para purificação de DNA plasmidial por meio de cromatografia baseada em ligantes de arginina
Co-supervisor
 Engenharia Química (PhD)
Universidade de São Paulo, Brazil
2014 - 2018 Affinity purification and cell-specific delivery of p53-encoding plasmid DNA for gene mediated cancer therapy (SFRH/BD/96809/2013)
Supervisor
 Bioquímica (PhD)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2016 - 2017 Pre-miR-29 as a lung cancer biopharmaceutical: a new approach for its purification and delivery with functionalized nanoparticles
Co-supervisor
 Tecnologias do Medicamento (Master)
Universidade de Coimbra, Portugal
2016 - 2017 Minicircular DNA as a potential gene therapy vector in cervical cancer
Co-supervisor
 Bioquímica (Master)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2016 - 2017 Novas estratégias de purificação de RNAs usando líquidos iónicos
Supervisor
 Biotecnologia (Master)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2016 - 2017 Recuperação de microRNA através de partículas magnéticas
Supervisor
 Ciências Biomédicas (Master)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2015 - 2016 miR-9 e miR-29 na doença de Alzheimer: Avaliação da possível sinergia
Supervisor
 Ciências Biomédicas (Master)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2015 - 2016 Construção de um vetor de expressão de Transtirretina
Co-supervisor
 Bioquímica (Master)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2015 - 2016 Bioreconhecimento de DNA minicircular por derivados de aminoácidos imobilizados em suportes monolíticos
Supervisor
 Biotecnologia. (Master)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2015 - 2016 Purification of plasmid DNA vaccine to prevention or treatment for cervical cancer
Co-supervisor
 Ciências Biomédicas (Master)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2015 - 2016 Understanding the interaction between pDNA and different chromatographic supports
Co-supervisor
 Bioquímica (Master)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2012 - 2016 miRNA-29 bioseparation and target delivery strategies for Alzheimer disease (SFRH/BD/81914/2011)
Supervisor
 Bioquímica (PhD)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2015 - 2015 Potencial terapêutico do microRNA-29 em diferentes patologias
Supervisor
 Bioquímica (Degree)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2015 - 2015 Avaliação da interação entre microRNAs e “long noncoding” RNAs e envolvimento na doença de Alzheimer
Supervisor
 Bioquímica (Degree)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2015 - 2015 Desenvolvimento de um processo biotecnológico para a preparação de “long non-coding” RNAs com aplicação terapêutica
Supervisor
 Biotecnologia (Degree)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2014 - 2015 Influência da tecnologia de purificação e estabilização na atividade biológica do miRNA
Supervisor
 Biotecnologia (Master)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2014 - 2015 Development of new methodologies for the purification of minicircle DNA using monolithic supports
Supervisor
 Bioquímica (Master)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2014 - 2015 Mitochondrial genetic therapy in Parkinson’s disease - Cloning mitochondrial gene ND1
Co-supervisor
 Biotecnologia (Master)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2014 - 2015 Purificação do pVAX-G4 utilizando suportes cromatográficos derivados de compostos heterocíclicos
Co-supervisor
 Biotecnologia (Master)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2014 - 2015 Obtaining a plasmid DNA vaccine against HPV
Co-supervisor
 Bioquímica (Master)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2011 - 2015 Biosynthesis, purification and delivery of minicircle DNA for gene therapy (SFRH/BD/80402/2011)
Supervisor
 Bioquímica (PhD)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2014 - 2014 Preparation and Purification of an oligonucleotide antisense for microRNA 106 silencing: a new approach to prevent colorectal cancer
Supervisor
 Biotecnologia (Degree)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2014 - 2014 Biological activity of Plasmid DNA and Minicircle DNA: a comparative study
Supervisor
 Bioquímica (Degree)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2013 - 2014 Identification of microRNA stabilizers to develop a formulation for Alzheimer’s Disease application
Supervisor
 Ciências Farmacêuticas (Master)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2013 - 2014 Development of new chromatographic supports for pDNA purification
Co-supervisor
 Biotecnologia (Master)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2013 - 2014 Transfection efficiency of pDNA in mitochondrial gene therapy
Co-supervisor
 Bioquímica (Master)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2013 - 2014 Purification of plasmid DNA HPV-16 E6/E7 DNA Vaccine and in vitro and in vivo immunogenicity evaluation
Co-supervisor
 Ciências Biomédicas (Master)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2010 - 2014 Biorecognition in amino acids affinity chromatography for RNA purification. (SFRH/BD/64100/2009)
Supervisor
 Bioquímica (PhD)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2012 - 2013 Development and optimization of a microRNA purification technology by affinity chromatography, using the concept of QbD
Supervisor
 Biotecnologia (Master)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2012 - 2013 Purification of HPV-16 E6/E7 plasmid DNA vaccine using a modified monolithic support
Co-supervisor
 Ciências Biomédicas (Master)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2012 - 2013 Development of a delivery vector for mitochondrial gene therapy
Co-supervisor
 Ciências Biomédicas (Master)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2012 - 2013 Development of a mini bioreactor platform for therapeutic plasmid DNA biosynthesis
Co-supervisor
 Biotecnologia (Master)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2012 - 2012 Development and evaluation of an mRNA vaccine
Supervisor
 Biotecnologia (Degree)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2012 - 2012 Development of a chromatographic strategy for miRNA purification
Supervisor
 Bioquímica (Degree)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2011 - 2012 Validation of an analytical method using an anion-exchange monolithic column for the assessment of supercoiled plasmid DNA
Supervisor
 Bioquímica (Master)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2011 - 2011 Development of a purification technology for the recovery of siRNA according to GMP guidelines
Supervisor
 Biotecnologia. (Degree)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2011 - 2011 RNA stabilizing agents for application in a biosynthesis and purification process
Supervisor
 Biotecnologia (Degree)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2010 - 2011 Development of new therapeutic strategies for controlled release of siRNA
Supervisor
 Bioquímica (Master)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2010 - 2011 Cyanines synthesis for application as ligands in affinity chromatography
Co-supervisor
 Bioquímica (Master)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2010 - 2010 Development of a biocompatible strategy for the isolation of iRNA for gene therapy application
Supervisor
 Bioquímica (Degree)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2010 - 2010 Nucleic acids stabilization for the improved performance of DNA vaccines
Supervisor
 Bioquímica (Degree)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2010 - 2010 Separation and purification of RNAs for potential application in gene therapy
Supervisor
 Bioquímica (Degree)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2009 - 2010 Development of a new vehicle to deliver plasmid DNA to eukaryotic cells
Co-supervisor
 Ciências Biomédicas. (Master)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2009 - 2010 Resveratrol recombinant production and study of its effect on urease inhibiton from Helicobacter pylori
Co-supervisor
 Bioquímica (Master)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2009 - 2010 Development of new protocols to purify antibody fragments by hydrophobic interaction chromatography for diagnostic purposes
Co-supervisor
 Ciências Biomédicas (Master)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2008 - 2009 Application of amino acids derivates as ligands in affinity chromatography for plasmid DNA purification
Supervisor
 Bioquímica (Master)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2008 - 2009 Purification of antibodies fragments by affinity and hidrophobic interaction chromatography
Co-supervisor
 Bioquímica (Master)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2008 - 2009 RNA purification by affinity chromatography using histidine immobilized
Supervisor
 Bioquímica (Master)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
Distinctions

Award

2023 ARRISCA C Business Ideas Contest, Innovation category - 3rd Place
2023 Concurso de Ideias de Negócio WIN-UBI, 1st Place
2023 UI.CAN Universidades como Interface para o Empreendedorismo - 2nd Place
2022 Best Paper Award