Ana Sofia de Sousa Valente Coroadinha (9B1A-3B6D-62AA)

Curriculum Vitae

Ana Sofia de Sousa Valente Coroadinha

Dr Ana Coroadinha is the director of the Cell Line Development and Molecular Virology (CLD&MV) lab since 2009 (at iBET and ITQB-NOVA institutes) where the principal area of scientific research activity is applied virology with the aim to provide therapeutic or prophylactic solutions for healthcare through the use of recombinant virus. Her research links virology and technology focused on the areas of gene therapy and vaccines. The work links molecular & synthetic biology, virology and cell biology (eukaryotic cells). We are interest in developing novel virus based biopharmaceuticals as well as tools for their research and manufacture. The group develops novel molecular switches, performs genetic enhancement of cells and virus, in order to improve either the product therapeutic efficacy (specificity, tropism, transduction efficiency, immune response) or the cell robustness (improved growth, survivability, metabolism, productivity, etc.). Currently the PI coordinates in the CLD&MV group 2 PhD Senior researchers, 1 Technician, 3 PhD students, 1 Junior researchers and 2 Master students. Dr Ana Sofia de Sousa Valente Coroadinha concluded her PhD in Chemical Engineering - Biotecnology - in 2005/10/06 (Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica) performed in a tripartite fellowship between Généthon (France), Helmholtz Centre for Infection Research (HZI) (germany) and iBET (Portugal). She holds a degree in Biochemistry (1999, Universidade de Lisboa Faculdade de Ciências). The PI has long track record, over 25 years, on the development of virus based biopharmaceuticals for vaccine and gene therapy applications. She published over 65 peer reviewed papers (Q1); 10 book chapters and received 4 awards. She was principal researcher of more than 20 projects (academic and industrial) and participated as member in several other national (e.g. FCT funded) and international (e.g. European Comission) funded projects. Additionally, the PI teaches at several master and PhD programs, as well, as in well regconized international workshops.

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Identification

Personal identification

Full name: Ana Sofia de Sousa Valente Coroadinha

Citation names

Coroadinha, AS
Coroadinha, Ana Sofia

Author identifiers

Ciência ID: 9B1A-3B6D-62AA
ORCID iD: 0000-0001-9728-6890

Email addresses

avalente@ibet.pt (Professional)
avalente@itqb.unl.pt (Professional)

Websites

http://www.itqb.unl.pt/research/technology/Cell Line Development and Molecular Biotechnology Laboratory (Professional)

Knowledge fields

Natural sciences - Biological Sciences - Virology
Natural sciences - Biological Sciences - Molecular Biology
Natural sciences - Biological Sciences - Biochemistry
Engineering and Technology - Industrial Biotechnology - Bioproducts Biomaterials
Engineering and Technology - Industrial Biotechnology - Bioprocessing Technologies
Medical and Health Sciences - Medical Biotechnology - Technologies Involving the Manipulation of Cells, Tissues, Organs or the Whole Organism

Languages

Language	Speaking	Reading	Writing	Listening	Peer-review
Portuguese (Mother tongue)
English	Advanced (C1)	Advanced (C1)	Advanced (C1)	Advanced (C1)	Advanced (C1)
French	Elementary (A2)	Elementary (A2)	Beginner (A1)	Intermediate (B1)	Elementary (A2)
Spanish; Castilian	Beginner (A1)	Elementary (A2)	Beginner (A1)	Elementary (A2)	Beginner (A1)

Education

	Degree	Classification
2005/10/06 Concluded	Chemical Engineering - Biotechnology (Doutoramento) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal "Improved Strategies for the Development of Retroviral Gene Therapy Vectors: from the producer cell to the viral vector" (THESIS/DISSERTATION)	Approved with unanimity
1999/01 Concluded	Biochemistry (Licenciatura) Universidade de Lisboa Faculdade de Ciências, Portugal "Regulação do Crescimento Celular pela Expressão do IRF-1 em Células BHK" (THESIS/DISSERTATION)	16

Affiliation

Science

	Category Host institution	Employer
2018/10/01 - Current	Principal Investigator (Research)	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal (...)
2015/01/01 - 2018/09/30	Auxiliary Researcher (Research)	Instituto de Biologia Experimental e Tecnológica, Portugal
	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal (...)
2005/12/01 - 2014/12	Auxiliary Researcher (Research)	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal (...)
2005/10/01 - 2005/11/30	Postdoc (Research)	Instituto de Biologia Experimental e Tecnológica, Portugal
	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal (...)
1999/01/01 - 2000/12/30	Researcher (Research)	Instituto de Biologia Experimental e Tecnológica, Portugal
	Instituto de Biologia Experimental e Tecnológica, Portugal (...)

Others

	Category Host institution	Employer
2009/07/01 - Current	Head of Cell Line Development and Molecular Virology Lab	Instituto de Biologia Experimental e Tecnológica, Portugal
	Instituto de Biologia Experimental e Tecnológica, Portugal
2009/07/01 - Current	Head of Cell Line Development and Molecular Virology Lab	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2012/05/01 - 2012/07/31	Visiting Researcher at Helmholtz Centre for Infection Research	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
	Helmholtz-Zentrum für Infektionsforschung GmbH, Germany
2011/08/01 - 2011/11/30	Visiting Researcher at Hannover Medical School	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
	Medizinische Hochschule Hannover Klinik für Hämatologie Hämostaseologie Onkologie und Stammzelltransplantation, Germany
2006/04/01 - 2006/09/30	Visiting Researcher at Institut de Génétique Moléculaire de Montpellier	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
	Institut de Génétique Moléculaire Montpellier, France
2004/07/01 - 2005/09/30	PhD student - at iBET (Portugal)	Fundação para a Ciência e a Tecnologia, Portugal
	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal (...)
2004/01/01 - 2004/06/30	PhD student at - HZI (Germany)	Fundação para a Ciência e a Tecnologia, Portugal
	Helmholtz-Zentrum für Infektionsforschung GmbH, Germany
2002/07/01 - 2003/12/31	PhD student - - at iBET (Portugal)	Fundação para a Ciência e a Tecnologia, Portugal
	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2001/01/01 - 2002/08/31	PhD student - at Généthon (France)	Fundação para a Ciência e a Tecnologia, Portugal
	Généthon, France
1997/09/01 - 1998/12/30	Diploma Student	Universidade de Lisboa Faculdade de Ciências, Portugal
	Instituto de Biologia Experimental e Tecnológica, Portugal

Projects

Grant

	Designation	Funders
2024/07/01 - 2026/06/30	NAAVIGATE - NOVEL ENGINEERING STRATEGIES TO UNBLOCK AAV VECTOR CELLULAR TRAFFICKING: n/a Principal investigator Instituto de Biologia Experimental e Tecnológica, Portugal	Instituto de Biologia Experimental e Tecnológica Ongoing
2023/01/01 - 2025/01/01	A process engineering approach for manufacturing CAR T cells in biOreactOrs with improved cLinical revelance – CARTool 2022.07312.PTDC Researcher Instituto de Biologia Experimental e Tecnológica, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2022/10/01 - 2023/10/31	GlyCOVID - Impact and infection of SARS-CoV-2 in Congenital Disorders of Glycosylation (CDG) n.a. Researcher Instituto de Biologia Experimental e Tecnológica, Portugal	Universidade Nova de Lisboa Concluded
2020 - 2021/11/30	ELEONOR - AAV vectors for Duchenne Muscular Dystrophies gene therapies n.a. Principal investigator Instituto de Biologia Experimental e Tecnológica, Portugal	Instituto de Biologia Experimental e Tecnológica Concluded
2018/10/01 - 2021/09/30	Accelerating Lentivirus manufacturing: an engineering driven approach PTDC/EQU-EPQ/29306/2017 Researcher Instituto de Biologia Experimental e Tecnológica, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2018/10/01 - 2021/09/30	Live cell sensors for virus detection: enabling drug and vaccine development for neglected diseases PTDC/BTM-SAL/30577/2017 Principal investigator Instituto de Biologia Experimental e Tecnológica, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2018/05/01 - 2021/04/30	Reclaimed wastewater for agriculture irrigation: mitigating microbiological hazards for a safer food production PTDC/CTA-AMB/29586/2017 Researcher Instituto de Biologia Experimental e Tecnológica, Portugal Instituto Nacional de Investigação Agrária e Veterinária, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2015 - 2020	DiViNE - Sustainable downstream processing of vaccines through incorporation of nanobiotechnologies: novel affinity ligands and biomimetic membranes H2020-LEIT-BIO-2014-1-635770 Researcher Instituto de Biologia Experimental e Tecnológica, Portugal	European Commission Concluded
2018 - 2019	DELIVER - Debottlenecking lentiviral vector manufacture n.a. Principal investigator Instituto de Biologia Experimental e Tecnológica, Portugal	Instituto de Biologia Experimental e Tecnológica Concluded
2013 - 2017	EDUFLUVAC - EDUcate inFLUenza VACcine – Combinatorial immunization strategy to educate the immune system towards cross recognition and coverage against antigenic drift in seasonal influenza virus exposure FP7-HEALTH-2013-INNOVATION-602640 Researcher Instituto de Biologia Experimental e Tecnológica, Portugal	European Commission Concluded
2012 - 2016	BRAINVECTORS -From Brain Gene Transfer Towards Gene Therapy: Pharmacological Assessment of AAV, CAV and LVV FP7-PEOPLE -2011-IAPP Grant agreement ID: 286071 Researcher Instituto de Biologia Experimental e Tecnológica, Portugal	European Commission Concluded
2012/01/01 - 2015/06/30	A novel generation of packaging cell lines for the manufacture of lentivirus based biopharmaceuticals PTDC/EBB-EBI/118621/2010 Principal investigator Instituto de Biologia Experimental e Tecnológica, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2012/03 - 2015/03	Development of improved suspension MDCK cell lines for the production of viral based bioproducts: manufacturing Influenza and Adenovirus info:eu-repo/grantAgreement/FCT/3599-PPCDT/118615/PT PTDC/EBB-EBI/118621/2010 Principal investigator Instituto de Biologia Experimental e Tecnológica, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2014/03 - 2015/02	Molecular approaches to develop an insect cell production system for Hepatitis C vaccine candidate. 134154 Provided by PTCRIS: 134154 Researcher	Fundação para a Ciência e a Tecnologia, I.P.
2011 - 2015	ComplexINC New technologies and production tools for complex protein biologicals HEALTH – 2011- 279039 Researcher Instituto de Biologia Experimental e Tecnológica, Portugal	European Commission Concluded
2010/04 - 2013/09	Using functional genomics to improve mammalian cells for virus based biopharmaceuticals manufacture 100491 Provided by PTCRIS: 100491 Principal investigator Instituto de Biologia Experimental e Tecnológica, Portugal	Fundação para a Ciência e a Tecnologia, I.P. Concluded
2011 - 2013	Viral Capture and Purification System: Smart macroporous structures for the affinity purification of retroviral particles PTDC/EBB-BIO/118317/2010 Researcher Instituto de Biologia Experimental e Tecnológica, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2011 - 2013	hCE-2 - expression and characterization in in vitro and in silico models PTDC/ EBB-BIO/111530/2009 Researcher Instituto de Biologia Experimental e Tecnológica, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2010 - 2013	Retroviral like particles: improving potential as candidate vaccines for Hepatitis C PTDC/EBB-BIO/102649/2008 Researcher Instituto de Biologia Experimental e Tecnológica, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2008 - 2013	BRAINCAV - Nonhuman adenovirus vectors for gene transfer to the Brain FP7-HEALTH-HS-2008-222992 Researcher Instituto de Biologia Experimental e Tecnológica, Portugal	European Commission Concluded
2010 - 2012	Novel cell factories for the production of secreted complex bioproducts: synthetic biology approaches for stoichiometric optimization PTDC/EBB-BIO/102266/2008 Researcher Instituto de Biologia Experimental e Tecnológica, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2006 - 2011	CLINIGENE - European Network for the Advancement of Clinical Gene Transfer and Therapy LSHB - CT-2006 -018933 Researcher Instituto de Biologia Experimental e Tecnológica, Portugal	European Commission Concluded
2007/01 - 2010/06	Manufacturing Canine Adenovirus Vectors for Gene Therapy: development of MDCK CAV-2 E1-complementing cell lines. 69452 Provided by PTCRIS: 69452 Principal investigator Instituto de Biologia Experimental e Tecnológica, Portugal	Fundação para a Ciência e a Tecnologia, I.P. Concluded
2007/04/01 - 2010/03/31	Development of flexible cell lines for the Production of Retrovirus and Lentivirus for Gene Therapy. info:eu-repo/grantAgreement/FCT/3599-PPCDT/69451/PT Principal investigator Instituto de Biologia Experimental e Tecnológica, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2008 - 2010	Chemical strategies for high-throughput proteomics: A rationally designed Affinity Tag Receptor as a new approach for the production, purification and immobilization of recombinant proteins PTDC/BIO/65383/2006 Researcher Instituto de Biologia Experimental e Tecnológica, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2005/05 - 2008/06	THERAVECT - Production, purification and storage of retroviral vectors produced by modular master producer cells: focus on CoIVII vectors for the Epidermolysis Bullosa therapy Provided by PTCRIS: 63789 63789 POCI/V.5/A0003/2005 Researcher Instituto de Biologia Experimental e Tecnológica, Portugal	Fundação para a Ciência e a Tecnologia, I.P. Fundação para a Ciência e a Tecnologia Concluded
2005 - 2008	Production, purification and storage of retroviral vectors produced by modular master producer cells: focus on ColVII vectors for the Epidermolysis Bullosa therapy POCI/V.5/A00003/2005 Researcher Instituto de Biologia Experimental e Tecnológica, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2002 - 2005	INSERTAGENE - Integrated Strategies for efficient therapeutic retrovirus based on modular cell lines- QLK3-CT-2002-01949 PhD Student Fellow Instituto de Biologia Experimental e Tecnológica, Portugal	European Commission
2001 - 2003	Stabilization of retroviruses for gene therapy clinical applications POCTI/BIO/35695/99 PhD Student Fellow Instituto de Biologia Experimental e Tecnológica, Portugal	Fundação para a Ciência e a Tecnologia Concluded

Contract

	Designation	Funders
2018 - Current	Development of coated VLPs for cancer research na Principal investigator Instituto de Biologia Experimental e Tecnológica, Portugal	Merck KGaA Ongoing
2022/10/01 - 2024/04/01	Deciphering Adeno-associated vectors 2 Principal investigator Instituto de Biologia Experimental e Tecnológica, Portugal	Boehringer Ingelheim Pharma GmbH & Co KG Biberach Ongoing
2023/01/01 - 2023/12/31	Lentiviral vector cells 1 Principal investigator Instituto de Biologia Experimental e Tecnológica, Portugal	CSL Behring GmbH Ongoing
2020/12/01 - 2020/12/31	HEK 293 Serum-free Suspension culture optimization n.a. Principal investigator Instituto de Biologia Experimental e Tecnológica, Portugal	Thermo Fisher Scientific Inc Concluded
2016 - 2020/12/31	Development of AAV vector expression systems n.a. Principal investigator Instituto de Biologia Experimental e Tecnológica, Portugal	Genzyme Corporation Concluded
2018 - 2019	Development of label free virus detection sensors n.a. Principal investigator Instituto de Biologia Experimental e Tecnológica, Portugal	Sanofi Pasteur Recherche Developpement Marcy l’Etoile Concluded
2017 - 2018	Development of lentiviral vectors for cell therapies n.a. Principal investigator Instituto de Biologia Experimental e Tecnológica, Portugal	Rubius Therapeutics Inc Concluded
2015 - 2017	Development of retroviral producer cell lines for CAR-T therapy n.a. Principal investigator Instituto de Biologia Experimental e Tecnológica, Portugal	UNUM Therapeutics Concluded
2015 - 2016	Development of transcomplementing Sf9 cell lines for Baculovirus Expression System. n.a. Principal investigator Instituto de Biologia Experimental e Tecnológica, Portugal	Généthon Concluded
2015 - 2015	Therapeutic recombinant protein manufacturing n.a. Principal investigator Instituto de Biologia Experimental e Tecnológica, Portugal	Nflection therapeutics Concluded
2013 - 2014	Development of coated VLPs n.a. Principal investigator Instituto de Biologia Experimental e Tecnológica, Portugal	Merck KGaA Concluded
2008 - 2008	Recombinant protein manufacturing for crystallographic studies n.a. Principal investigator Instituto de Biologia Experimental e Tecnológica, Portugal	University of Dublin Trinity College

Outputs

Publications

Book chapter

Coroadinha, Ana S.. "Cancer Gene Therapy: Development and Production of Lentiviral Vectors for Gene Therapy". In Methods in Molecular Biology, 297-315. Springer US, 2022.
Published • 10.1007/978-1-0716-2441-8_16
Ana S. Coroadinha; Pedro Viana Batista. "Nanobiotechnology tools for diagnosis and therapy.". In From Life Molecules to global Health, 131-143. Portugal: Principia Editora, 2021.
Published
Fernandes, P.; Silva, A.C.; Coroadinha, A.S.; Alves, P.M.. "Upstream Bioprocess for Adenovirus Vectors". In In D.T. Curriel (ed.) Adenoviral Vectors for Gene Therapy, 139-161. 2016.
Published • 10.1016/B978-0-12-800276-6.00006-1
Vidigal, J; Fernandes, Fabiana; Coroadinha, Ana Sofia; Teixeira, Ana Margarida; Alves, Paula Marques. "Insect cell line development using FLP-mediated cassette exchange technology". In Methods Mol Biol., 15-27. 2014.
Published
Simplício, A.L.; Coroadinha, A.S.; Gilmer, J.F.; Lamego, J.; Simplício AL; Coroadinha AS; Gilmer JF; Lamego J. "A methodology for detection and quantification of esterase activity". In Capillary Electrophoresis of Biomolecules: Methods and Protocols, 309-319. 2013.
Published • 10.1007/978-1-62703-296-4-22
A., Helio; F., Ana; M., Paula; S., Ana. "Lentiviral Gene Therapy Vectors: Challenges and Future Directions". In Gene Therapy - Tools and Potential Applications, edited by Martin, Francisco, 287-317. InTech, 2013.
Published • 10.5772/52534
Coroadinha, Ana; Wirth, D; Rodrigues, Ana Filipa; Duros, C; Cohen-Haguenauer, Odile; Alves, PA; Cruz, PE; Carrondo, MJT; Hauser, H.. "Modular retroviral producer cell lines". In The CliniBook: Clinical gene transfer, edited by Odile Cohen-Haguenauer, 118. EDK, Paris ©, 2012.
Published
Bandeira, V; Peixoto, Cristina; Coroadinha, Ana; Cruz, Pedro; Carrondo, MJT; Merten, O.-W.; Alves, PA. "Facing the challenges of downstream processing of lentiviral vectors". In The CliniBook: Clinical gene transfer, edited by Odile Cohen-Haguenauer, 150. EDK, Paris ©, 2012.
Published
Rodrigues, Ana; M., Paula; Coroadinh, Ana. "Production of Retroviral and Lentiviral Gene Therapy Vectors: Challenges in the Manufacturing of Lipid Enveloped Virus". In Viral Gene Therapy, edited by Xu, Ke, 15-40. InTech, 2011.
10.5772/18615
Cruz, Pedro; Rodrigues, Teresa; Carmo, Marlene; Wirth, D; Amaral, Ana Isabel; Alves, Paula Marques; Coroadinha, Ana Sofia. "Manufacturing of Retroviruses". In Methods in Molecular Biology, Viral Vectors for Gene Therapy, 157-182. Humana Press, 2011.
Published
Cruz, Pedro; Coroadinha, Ana; Rodrigues, Ana Filipa; Hauser, H.. "Production of Retroviral Vectors: from the producer cell to the final product". In Gene and Cell Therapy: Therapeutic Mechanisms and Strategies, 17-32. Taylor & Francis (CRC Press), 2008.
Published

Journal article

Formas-Oliveira, Ana S.; Ferreira, Mariana Valentim; Coroadinha, Ana Sofia. Corresponding author: Coroadinha, Ana Sofia. "Deciphering key parameters potentiating high yields in lentiviral vector stable cell lines: analysis of vector components copy number and expression levels". Molecular Therapy Methods & Clinical Development (2025): 101431. https://doi.org/10.1016/j.omtm.2025.101431.
10.1016/j.omtm.2025.101431
Tiago A. Vaz; Ana F. Rodrigues; Ana S. Coroadinha. "Exploring nutrient supplementation and bioprocess optimization to improve the production of lentiviral vectors in serum-free medium suspension cultures". Biotechnology Journal (2024): https://doi.org/10.1002/biot.202300212.
10.1002/biot.202300212
Bernardino, Thaissa Consoni. "Virus Hybrid Vectored Particles Carrying an RNA Coding for Rabies Virus Glycoprotein". APCRI Journal 25 02 (2023): 1-7. https://doi.org/10.24321/0973.5038.202308.
10.24321/0973.5038.202308
Mariana V. Ferreira; Sofia Fernandes; Ana Isabel Almeida; Salomé Neto; João P. Mendes; Ricardo J. S. Silva; Cristina Peixoto; Ana Sofia Coroadinha. "Extending AAV Packaging Cargo through Dual Co-Transduction: Efficient Protein Trans-Splicing at Low Vector Doses". International Journal of Molecular Sciences (2023): https://doi.org/10.3390/ijms241310524.
10.3390/ijms241310524
Sofia Fernandes; Joana Diogo; Ana Sofia Coroadinha. "Mammalian Stable Cell Platforms for Recombinant Adeno-associated Virus (rAAV) Production: Development Strategies and their Impact on Viral Productivity". Current Gene Therapy (2023): https://doi.org/10.2174/1566523223666230331111336.
10.2174/1566523223666230331111336
Ana Sofia Coroadinha. "Host Cell Restriction Factors Blocking Efficient Vector Transduction: Challenges in Lentiviral and Adeno-Associated Vector Based Gene Therapies". Cells (2023): https://doi.org/10.3390/cells12050732.
10.3390/cells12050732
Ana Sofia Moreira; Sandra Bezemer; Tiago Q. Faria; Frank Detmers; Pim Hermans; Laurens Sierkstra; Ana Sofia Coroadinha; Cristina Peixoto. "Implementation of Novel Affinity Ligand for Lentiviral Vector Purification". International Journal of Molecular Sciences (2023): https://doi.org/10.3390/ijms24043354.
10.3390/ijms24043354
Mariana V. Ferreira; Elisa T. Cabral; Ana Sofia Coroadinha. "Progress and Perspectives in the Development of Lentiviral Vector Producer Cells". Biotechnology Journal 16 1 (2021): 2000017-2000017. https://doi.org/10.1002/biot.202000017.
10.1002/biot.202000017
Miguel R. Guerreiro; Ana R. Fernandes; Ana S. Coroadinha. "Evaluation of Structurally Distorted Split GFP Fluorescent Sensors for Cell-Based Detection of Viral Proteolytic Activity". Sensors (2021): https://doi.org/10.3390/s21010024.
Published • 10.3390/s21010024
Ana S. Formas-Oliveira; João S. Basílio; Ana F. Rodrigues; Ana S. Coroadinha. "Overexpression of ER Protein Processing and Apoptosis Regulator Genes in Human Embryonic Kidney 293 Cells Improves Gene Therapy Vectors Production". Biotechnology Journal 15 9 (2020): 1900562-1900562. https://doi.org/10.1002/biot.201900562.
10.1002/biot.201900562
Ana Filipa Rodrigues; Paulo Fernandes; Tanja Laske; Rute Castro; Paula Marques Alves; Yvonne Genzel; Ana Sofia Coroadinha. "Cell Bank Origin of MDCK Parental Cells Shapes Adaptation to Serum-Free Suspension Culture and Canine Adenoviral Vector Production". International Journal of Molecular Sciences 21 17 (2020): 6111-6111. https://doi.org/10.3390/ijms21176111.
10.3390/ijms21176111
Mateusz Marchel; Ana Sofia Coroadinha; Isabel M. Marrucho. "Novel Acidic Deep Eutectic Solvent-Based Aqueous Biphasic Systems for Efficient Extraction of Pepsin". ACS Sustainable Chemistry & Engineering (2020): https://doi.org/10.1021/acssuschemeng.0c02673.
10.1021/acssuschemeng.0c02673
Ana Sofia Moreira; Ana Carina Silva; Marcos F. Q. Sousa; Åsa Hagner-McWhirterc; Gustaf Ahlénc; Mats Lundgren; Ana S. Coroadinha; et al. "Establishing Suspension Cell Cultures for Improved Manufacturing of Oncolytic Adenovirus". Biotechnology Journal (2020): 1900411-1900411. https://doi.org/10.1002/biot.201900411.
10.1002/biot.201900411
Soares HR; Ferreira-Fernandes M; Almeida AI; Marchel M; Alves PM; Coroadinha AS. "Enhancing Hepatitis C virus pseudoparticles infectivity through p7NS2 cellular expression.". Journal of virological methods (2019): http://europepmc.org/abstract/med/31412271.
10.1016/j.jviromet.2019.113714
Suárez-Patiño SF; Bernardino TC; Núñez EGF; Astray RM; Pereira CA; Soares HR; Coroadinha AS; Jorge SAC. "Semliki Forest Virus replicon particles production in serum-free medium BHK-21 cell cultures and their use to express different proteins.". Cytotechnology (2019): http://europepmc.org/abstract/med/31422494.
10.1007/s10616-019-00337-y
Tomás HA; Mestre DA; Rodrigues AF; Guerreiro MR; Carrondo MJT; Coroadinha AS. "Improved GaLV-TR Glycoproteins to Pseudotype Lentiviral Vectors: Impact of Viral Protease Activity in the Production of LV Pseudotypes.". Molecular therapy. Methods & clinical development (2019): http://europepmc.org/abstract/med/31528654.
10.1016/j.omtm.2019.08.001
Coroadinha, Ana. "High-throughput screening of aqueous biphasic systems with ionic liquids as additives for extraction and purification of enveloped virus-like particles". Engineering Reports (2019): http://dx.doi.org/10.1002/eng2.12030.
10.1002/eng2.12030
Catarina Pinto; Gabriela Silva; Ana S. Ribeiro; Mónica Oliveira; Manuel Garrido; Vanessa S. Bandeira; André Nascimento; et al. "Evaluation of AAV-mediated delivery of shRNA to target basal-like breast cancer genetic vulnerabilities". Journal of Biotechnology 300 (2019): 70-77. https://doi.org/10.1016/j.jbiotec.2019.05.016.
10.1016/j.jbiotec.2019.05.016
Miguel Ricardo Guerreiro; Daniela Filipa Freitas; Paula Marques Alves; Ana Sofia Coroadinha. "Detection and Quantification of Label-Free Infectious Adenovirus Using a Switch-On Cell-Based Fluorescent Biosensor". ACS Sensors (2019): https://doi.org/10.1021/acssensors.9b00489.
10.1021/acssensors.9b00489
Soares HR; Castro R; Tomás HA; Carrondo MJT; Alves PM; Coroadinha AS. "Pseudotyping retrovirus like particles vaccine candidates with Hepatitis C virus envelope protein E2 requires the cellular expression of CD81.". AMB Express (2019): http://europepmc.org/abstract/med/30729353.
10.1186/s13568-019-0741-5
Nina Schmolka; Pedro H. Papotto; Paula Vargas Romero; Tiago Amado; Francisco J. Enguita; Ana Amorim; Ana F. Rodrigues; et al. "MicroRNA-146a controls functional plasticity in ¿d T cells by targeting NOD1". Science Immunology (2018): https://doi.org/10.1126/sciimmunol.aao1392.
10.1126/sciimmunol.aao1392
Hugo R. Soares; Ana I. Almeida; Hélio A. Tomás; Paula M. Alves; Ana S. Coroadinha. "Flexible pseudotyping of retrovirus using recombinase-mediated cassette exchange". Biotechnology Letters 40 4 (2018): 633-639. https://doi.org/10.1007/s10529-018-2515-6.
10.1007/s10529-018-2515-6
Tomás HA; Rodrigues AF; Carrondo MJT; Coroadinha AS. "LentiPro26: novel stable cell lines for constitutive lentiviral vector production.". Scientific reports (2018): http://europepmc.org/abstract/med/29588490.
10.1038/s41598-018-23593-y
Lemos MAN; Patiño SFS; Bernardino TC; Coroadinha AS; Soares H; Astray RM; Pereira CA; Jorge SAC. "Intracellular Delivery of HCV NS3p gene using vectored particles.". Journal of biotechnology (2018): http://europepmc.org/abstract/med/29577966.
10.1016/j.jbiotec.2018.03.010
Bandeira, V.S.; Tomás, H.A.; Alici, E.; Carrondo, M.J.T.; Coroadinha, A.S.. "Disclosing the Parameters Leading to High Productivity of Retroviral Producer Cells Lines: Evaluating Random Versus Targeted Integration". Human Gene Therapy Methods 28 2 (2017): 78-90. http://www.scopus.com/inward/record.url?eid=2-s2.0-85017406682&partnerID=MN8TOARS.
10.1089/hgtb.2016.149
Fernandes CS; Castro R; Coroadinha AS; Roque AC. "Small synthetic ligands for the enrichment of viral particles pseudotyped with amphotropic murine leukemia virus envelope.". J Chromatogr A 1438 (2016): 160. http://europepmc.org/abstract/med/26903473.
Published • 10.1016/j.chroma.2016.02.026
Soares HR; Castro R; Tomás HA; Rodrigues AF; Gomes-Alves P; Bellier B; Klatzmann D; et al. "Tetraspanins displayed in retrovirus-derived virus-like particles and their immunogenicity.". Vaccine 34 13 (2016): 1. http://europepmc.org/abstract/med/26795367.
Published • 10.1016/j.vaccine.2015.12.015
Patrone M; Coroadinha AS; Teixeira AP; Alves PM. "Palmitoylation Strengthens Cholesterol-dependent Multimerization and Fusion Activity of Human Cytomegalovirus Glycoprotein B (gB).". J Biol Chem. 291 9 (2016): 471. http://europepmc.org/abstract/med/26694613.
Published • 10.1074/jbc.M115.682252
Rodrigues AF; Guerreiro MR; Formas-Oliveira AS; Fernandes P; Blechert AK; Genzel Y; Alves PM; Hu WS; Coroadinha AS. "Increased titer and reduced lactate accumulation in recombinant retrovirus production through the down-regulation of HIF1 and PDK.". Biotechnol Bioeng. 113 1 (2016): 150. http://europepmc.org/abstract/med/26134455.
Published • 10.1002/bit.25691
Patinha, D.J.S.; Tomé, L.C.; Florindo, C.; Soares, H.R.; Coroadinha, A.S.; Marrucho, I.M.. "New Low-Toxicity Cholinium-Based Ionic Liquids with Perfluoroalkanoate Anions for Aqueous Biphasic System Implementation". ACS Sustainable Chemistry and Engineering 4 5 (2016): 2670-2679. http://www.scopus.com/inward/record.url?eid=2-s2.0-84967153287&partnerID=MN8TOARS.
10.1021/acssuschemeng.6b00171
Alves, M.; Lamego, J.; Bandeiras, T.; Castro, R.; Tomás, H.; Coroadinha, A.S.; Costa, J.; Simplício, A.L.. "Human carboxylesterase 2: Studies on the role of glycosylation for enzymatic activity". Biochemistry and Biophysics Reports 5 (2016): 105-110. http://www.scopus.com/inward/record.url?eid=2-s2.0-84949187250&partnerID=MN8TOARS.
10.1016/j.bbrep.2015.11.018
Pina, A.S.; Carvalho, S.; Dias, A.M.G.C.; Guilherme, M.; Pereira, A.S.; Caraça, L.T.; Coroadinha, A.S.; Lowe, C.R.; Roque, A.C.A.. "Tryptophan tags and de novo designed complementary affinity ligands for the expression and purification of recombinant proteins". Journal of Chromatography A 1472 (2016): 55-65. http://www.scopus.com/inward/record.url?eid=2-s2.0-84994476942&partnerID=MN8TOARS.
10.1016/j.chroma.2016.10.017
Fernandes, C.S.M.; Barbosa, I.; Castro, R.; Pina, A.S.; Coroadinha, A.S.; Barbas, A.; Roque, A.C.A.. "Retroviral particles are effectively purified on an affinity matrix containing peptides selected by phage-display". Biotechnology Journal 11 12 (2016): 1513-1524. http://www.scopus.com/inward/record.url?eid=2-s2.0-84995387896&partnerID=MN8TOARS.
10.1002/biot.201600025
Carinhas N; Pais DA; Koshkin A; Fernandes P; Coroadinha AS; Carrondo MJ; Alves PM; Teixeira AP. "Metabolic flux profiling of MDCK cells during growth and canine adenovirus vector production.". Sci Rep. 23 6 (2016): 23529. http://europepmc.org/abstract/med/27004747.
Published • 10.1038/srep23529
Rodrigues AF; Soares HR; Guerreiro MR; Alves PM; Coroadinha AS. "Viral vaccines and their manufacturing cell substrates: New trends and designs in modern vaccinology.". Biotechnol. J. 10 9 (2015): 13. http://europepmc.org/abstract/med/26212697.
Published • 10.1002/biot.201400387
Silva MM; Rodrigues AF; Correia C; Sousa MF; Brito C; Coroadinha AS; Serra M; Alves PM. "Robust Expansion of Human Pluripotent Stem Cells: Integration of Bioprocess Design With Transcriptomic and Metabolomic Characterization.". Stem Cells and Translational Medicine 4 7 (2015): 731. http://europepmc.org/abstract/med/25979863.
Published • 10.5966/sctm.2014-0270
Rodrigues AF; Formas-Oliveira AS; Guerreiro MR; Tomás HA; Alves PM; Coroadinha AS. "Single step cloning-screening method:A new tool for developing and studying high-titer viral vector producer cells.". Gene Ther. 22 9 (2015): 68. http://europepmc.org/abstract/med/25938191.
Published • 10.1038/gt.2015.44
Tomé, L.C.; Silva, N.H.C.S.; Soares, H.R.; Coroadinha, A.S.; Sadocco, P.; Marrucho, I.M.; Freire, C.S.R.. "Bioactive transparent films based on polysaccharides and cholinium carboxylate ionic liquids". Green Chemistry 17 8 (2015): 4291-4299. http://www.scopus.com/inward/record.url?eid=2-s2.0-84938486603&partnerID=MN8TOARS.
10.1039/c5gc00416k
Fernandes, P.; Almeida, A.I.; Kremer, E.J.; Alves, P.M.; Coroadinha, A.S.. "Canine helper-dependent vectors production: Implications of Cre activity and co-infection on adenovirus propagation". Scientific Reports 5 (2015): 1-8. http://www.scopus.com/inward/record.url?eid=2-s2.0-84924985015&partnerID=MN8TOARS.
10.1038/srep09135
Castro, R.; Fernandes, P.; Laske, T.; Sousa, M.F.Q.; Genzel, Y.; Scharfenberg, K.; Alves, P.M.; Coroadinha, A.S.. "Production of canine adenovirus type 2 in serum-free suspension cultures of MDCK cells". Applied Microbiology and Biotechnology (2015): http://www.scopus.com/inward/record.url?eid=2-s2.0-84929678417&partnerID=MN8TOARS.
10.1007/s00253-015-6636-8
Fernandes P; Simão D; Guerreiro MR; Kremer EJ; Coroadinha AS; Alves PM. "Impact of adenovirus life cycle progression on the generation of canine helper-dependent vectors.". Gene Therapy 22 1 (2014): 40. http://europepmc.org/abstract/med/25338917.
Published • 10.1038/gt.2014.92
Vidigal J; Fernandes F; Coroadinha AS; Teixeira AP; Alves PM; Vidigal, J.; Fernandes, F.; et al. "Insect cell line development using FLP-mediated cassette exchange technology.". Methods Mol Biol. 1104 15 (2014): http://europepmc.org/abstract/med/24297406.
Published • 10.1007/978-1-62703-733-4_2
Rodrigues, A.F.; Carrondo, M.J.T.; Alves, P.M.; Coroadinha, A.S.. "Cellular targets for improved manufacturing of virus-based biopharmaceuticals in animal cells". Trends in Biotechnology 32 12 (2014): 602-607. http://www.scopus.com/inward/record.url?eid=2-s2.0-84918492393&partnerID=MN8TOARS.
10.1016/j.tibtech.2014.09.010
Fernandes, Paulo; Castro, Rute; Alves, Paula Marques; Carrondo, MJT; Coroadinha, Ana. "Terapia génica e desafios no desenvolvimento de vectores virais". Boletin Biotecnologia 4 (2013): 21-23.
Published
Rodrigues, A.F.; Formas-Oliveira, A.S.; Bandeira, V.S.; Alves, P.M.; Hu, W.S.; Coroadinha, A.S.. "Metabolic pathways recruited in the production of a recombinant enveloped virus: Mining targets for process and cell engineering". Metabolic Engineering 20 (2013): 131-145. http://www.scopus.com/inward/record.url?eid=2-s2.0-84886481436&partnerID=MN8TOARS.
10.1016/j.ymben.2013.10.001
Fernandes, P.; Santiago, V.M.; Rodrigues, A.F.; Tomás, H.; Kremer, E.J.; Alves, P.M.; Coroadinha, A.S.. "Impact of E1 and Cre on Adenovirus Vector Amplification: Developing MDCK CAV-2-E1 and E1-Cre Transcomplementing Cell Lines". PLoS ONE 8 4 (2013): http://www.scopus.com/inward/record.url?eid=2-s2.0-84875641729&partnerID=MN8TOARS.
10.1371/journal.pone.0060342
Fernandes, P.; Peixoto, C.; Santiago, V.M.; Kremer, E.J.; Coroadinha, A.S.; Alves, P.M.. "Bioprocess development for canine adenovirus type 2 vectors". Gene Therapy 20 4 (2013): 353-360. http://www.scopus.com/inward/record.url?eid=2-s2.0-84875922065&partnerID=MN8TOARS.
10.1038/gt.2012.52
Lamego, J.; Cunha, B.; Peixoto, C.; Sousa, M.F.; Alves, P.M.; Simplício, A.L.; Coroadinha, A.S.. "Carboxylesterase 2 production and characterization in human cells: New insights into enzyme oligomerization and activity". Applied Microbiology and Biotechnology 97 3 (2013): 1161-1173. http://www.scopus.com/inward/record.url?eid=2-s2.0-84874116579&partnerID=MN8TOARS.
10.1007/s00253-012-3994-3
Fernandes, F.; Vidigal, J.; Dias, M.M.; Prather, K.L.; Coroadinha, A.S.; Teixeira, A.P.; Alves, P.M.. "Flipase-mediated cassette exchange in Sf9 insect cells for stable gene expression". Biotechnology and Bioengineering 109 11 (2012): 2836-2844. http://www.scopus.com/inward/record.url?eid=2-s2.0-84866751772&partnerID=MN8TOARS.
10.1002/bit.24542
Bandeira, V.; Peixoto, C.; Rodrigues, A.F.; Cruz, P.E.; Alves, P.M.; Coroadinha, A.S.; Carrondo, M.J.T.. "Downstream processing of lentiviral vectors: Releasing bottlenecks". Human Gene Therapy Methods 23 4 (2012): 255-263. http://www.scopus.com/inward/record.url?eid=2-s2.0-84872231059&partnerID=MN8TOARS.
10.1089/hgtb.2012.059
Rodrigues, A.F.; Amaral, A.I.; Veríssimo, V.; Alves, P.M.; Coroadinha, A.S.. "Adaptation of retrovirus producer cells to serum deprivation: Implications in lipid biosynthesis and vector production". Biotechnology and Bioengineering 109 5 (2012): 1269-1279. http://www.scopus.com/inward/record.url?eid=2-s2.0-84858449640&partnerID=MN8TOARS.
10.1002/bit.24410
Rodrigues AF; Guerreiro MR; Castro R; Tomás HA; Dalba C; Klatzmann D; Alves PM; et al. "Down-regulation of CD81 in human cells producing HCV-E1/E2 retroVLPs.". Biotechnol. Bioeng. 108 11 (2011): 2623. http://europepmc.org/abstract/PMC/PMC3284910.
Published • 10.1186/1753-6561-5-S8-P72
Rodrigues, A.F.; Guerreiro, M.R.; Santiago, V.M.; Dalba, C.; Klatzmann, D.; Alves, P.M.; Carrondo, M.J.T.; Coroadinha, A.S.. "Down-regulation of CD81 tetraspanin in human cells producing retroviral-based particles: Tailoring vector composition". Biotechnology and Bioengineering 108 11 (2011): 2623-2633. http://www.scopus.com/inward/record.url?eid=2-s2.0-80053052764&partnerID=MN8TOARS.
10.1002/bit.23231
Cruz, P.E.; Rodrigues, T.; Carmo, M.; Wirth, D.; Amaral, A.I.; Alves, P.M.; Coroadinha, A.S.. "Manufacturing of retroviruses.". Methods in molecular biology (Clifton, N.J.) 737 (2011): 157-182. http://www.scopus.com/inward/record.url?eid=2-s2.0-80052323923&partnerID=MN8TOARS.
10.1007/978-1-61779-095-9_7
Carrondo, M.; Panet, A.; Wirth, D.; Coroadinha, A.S.; Cruz, P.; Falk, H.; Schucht, R.; et al. "Integrated strategy for the production of therapeutic retroviral vectors". Human Gene Therapy 22 3 (2011): 370-379. http://www.scopus.com/inward/record.url?eid=2-s2.0-79952650338&partnerID=MN8TOARS.
10.1089/hum.2009.165
Lamego, J.; Coroadinha, A.S.; Simplício, A.L.. "Detection and quantification of carboxylesterase 2 activity by capillary electrophoresis". Analytical Chemistry 83 3 (2011): 881-887. http://www.scopus.com/inward/record.url?eid=2-s2.0-79952151285&partnerID=MN8TOARS.
10.1021/ac102547c
Coroadinha, A.S.; Gama-Norton, L.; Amaral, A.I.; Hauser, H.; Alves, P.M.; Cruz, P.E.. "Production of Retroviral Vectors: Review". Current Gene Therapy 10 6 (2010): 456-473. http://www.scopus.com/inward/record.url?eid=2-s2.0-78651328530&partnerID=MN8TOARS.
10.2174/156652310793797739
Gama-Norton, L.; Herrmann, S.; Schucht, R.; Coroadinha, A.S.; Löw, R.; Alves, P.M.; Bartholomae, C.C.; et al. "Retroviral vector performance in defined chromosomal loci of modular packaging cell lines". Human Gene Therapy 21 8 (2010): 979-991. http://www.scopus.com/inward/record.url?eid=2-s2.0-77955506625&partnerID=MN8TOARS.
10.1089/hum.2009.089
Rodrigues, A.F.; Carmo, M.; Alves, P.M.; Coroadinha, A.S.. "Retroviral vector production under serum deprivation: The role of lipids". Biotechnology and Bioengineering 104 6 (2009): 1171-1181. http://www.scopus.com/inward/record.url?eid=2-s2.0-70449119110&partnerID=MN8TOARS.
10.1002/bit.22499
Carmo, M.; Alves, A.; Rodrigues, A.F.; Coroadinha, A.S.; Teixeira Carrondo, M.J.; Alves, P.M.; Cruz, P.E.. "Stabilization of gammaretroviral and lentiviral vectors: From production to gene transfer". Journal of Gene Medicine 11 8 (2009): 670-678. http://www.scopus.com/inward/record.url?eid=2-s2.0-70349492116&partnerID=MN8TOARS.
10.1002/jgm.1353
Carmo, M.; Dias, J.D.; Panet, A.; Coroadinha, A.S.; Carrondo, M.J.; Alves, P.M.; Cruz, P.E.; et al. "Thermosensitivity of the reverse transcription process as an inactivation mechanism of lentiviral vectors.". Human gene therapy 20 10 (2009): 1168-1176. http://www.scopus.com/inward/record.url?eid=2-s2.0-75349098046&partnerID=MN8TOARS.
10.1089/hum.2009.068
Amaral, A.I.; Coroadinha, A.S.; Merten, O.-W.; Alves, P.M.. "Improving retroviral vectors production: Role of carbon sources in lipid biosynthesis". Journal of Biotechnology 138 3-4 (2008): 57-66. http://www.scopus.com/inward/record.url?eid=2-s2.0-55049100170&partnerID=MN8TOARS.
10.1016/j.jbiotec.2008.08.005
Carrondo, M.J.T.; Merten, O.-W.; Haury, M.; Alves, P.M.; Coroadinha, A.S.. "Impact of retroviral vector components stoichiometry on packaging cell lines: Effects on productivity and vector quality". Human Gene Therapy 19 2 (2008): 199-210. http://www.scopus.com/inward/record.url?eid=2-s2.0-39449115121&partnerID=MN8TOARS.
10.1089/hum.2007.0101
Coroadinha, A.S.; Silva, A.C.; Pires, E.; Coelho, A.; Alves, P.M.; Carrondo, M.J.T.. "Effect of osmotic pressure on the production of retroviral vectors: Enhancement in vector stability". Biotechnology and Bioengineering 94 2 (2006): 322-329. http://www.scopus.com/inward/record.url?eid=2-s2.0-33744474819&partnerID=MN8TOARS.
10.1002/bit.20847
Coroadinha, A.S.; Ribeiro, J.; Roldão, A.; Cruz, P.E.; Alves, P.M.; Merten, O.-W.; Carrondo, M.J.T.. "Effect of medium sugar source on the production of retroviral vectors for gene therapy". Biotechnology and Bioengineering 94 1 (2006): 24-36. http://www.scopus.com/inward/record.url?eid=2-s2.0-33646551820&partnerID=MN8TOARS.
10.1002/bit.20778
Carmo, M.; Faria, T.Q.; Falk, H.; Coroadinha, A.S.; Teixeira, M.; Merten, O.-W.; Gény-Fiamma, C.; et al. "Relationship between retroviral vector membrane and vector stability". Journal of General Virology 87 5 (2006): 1349-1356. http://www.scopus.com/inward/record.url?eid=2-s2.0-33646148048&partnerID=MN8TOARS.
10.1099/vir.0.81302-0
Coroadinha, A.S.; Alves, P.M.; Santos, S.S.; Cruz, P.E.; Merten, O.-W.; Carrondo, M.J.T.. "Retrovirus producer cell line metabolism: Implications on viral productivity". Applied Microbiology and Biotechnology 72 6 (2006): 1125-1135. http://www.scopus.com/inward/record.url?eid=2-s2.0-33749838663&partnerID=MN8TOARS.
10.1007/s00253-006-0401-y
Schucht, R.; Coroadinha, A.S.; Zanta-Boussif, M.A.; Verhoeyen, E.; Carrondo, M.J.T.; Hauser, H.; Wirth, D.. "A New Generation of Retroviral Producer Cells: Predictable and Stable Virus Production by Flp-Mediated Site-Specific Integration of Retroviral Vectors". Molecular Therapy 14 2 (2006): 285-292. http://www.scopus.com/inward/record.url?eid=2-s2.0-33745668475&partnerID=MN8TOARS.
10.1016/j.ymthe.2005.12.003
Coroadinha, A.S.; Schucht, R.; Gama-Norton, L.; Wirth, D.; Hauser, H.; Carrondo, M.J.T.. "The use of recombinase mediated cassette exchange in retroviral vector producer cell lines: Predictability and efficiency by transgene exchange". Journal of Biotechnology 124 2 (2006): 457-468. http://www.scopus.com/inward/record.url?eid=2-s2.0-33745220778&partnerID=MN8TOARS.
10.1016/j.jbiotec.2006.01.037
Carmo, M.; Peixoto, C.; Coroadinha, A.S.; Alves, P.M.; Cruz, P.E.; Carrondo, M.J.T.. "Quantitation of MLV-based retroviral vectors using real-time RT-PCR". Journal of Virological Methods 119 2 (2004): 115-119. http://www.scopus.com/inward/record.url?eid=2-s2.0-2442638707&partnerID=MN8TOARS.
10.1016/j.jviromet.2004.03.007
Maranga, L.; Coroadinha, A.S.; Carrondo, M.J.T.. "Insect cell culture medium supplementation with fetal bovine serum and bovine serum albumin: Effects on baculovirus adsorption and infection kinetics". Biotechnology Progress 18 4 (2002): 855-861. http://www.scopus.com/inward/record.url?eid=2-s2.0-0036022009&partnerID=MN8TOARS.
10.1021/bp025514b
Carvalhal, A.V.; Coroadinha, A.S.; Alves, P.M.; Moreira, J.L.; Hauser, H.; Carrondo, M.J.T.. "Metabolic changes during cell growth inhibition by the IRF-1 system". Enzyme and Microbial Technology 30 1 (2002): 95-109. http://www.scopus.com/inward/record.url?eid=2-s2.0-0037039483&partnerID=MN8TOARS.
10.1016/S0141-0229(01)00460-4

Intellectual property

Patent	Hélio A. Tomás; Daniel Mestre; Ana Filipa Rodrigues; Coroadinha, Ana Sofia. 2020. "MODIFIED ENVELOPE GLYCOPROTEINS FOR RETROVIRIDAE VIRAL VECTORS". Pending
Provisional application for patent	2022. "PROTEASE-CONDITIONAL TARGETED NUCLEIC ACID RECOMBINATION, METHOD AND USES THEREOF". Protected 2019. "MODIFIED ENVELOPE GLYCOPROTEINS FOR RETROVIRIDAE VIRAL VECTOR PSEUDOTYPING AND PROCESS FOR OBTINING IT". Protected 2012. "STABLE GENE EXPRESSION IN SPODOPTERA FRUGIPERDA CELLS THROUGH RECOMBINASE MEDIATED CASSETTE EXCHANGE". Protected

Activities

Oral presentation

	Presentation title	Event name Host (Event location)
2024/10/01	Extending AAV genome cargo: delivering large therapeutic genes to treat Duchenne Muscular Dystrophies	ISCT Europe ISCT (Gothenburg , Sweden)
2024/03/01	Challenges in AAV Vectors-Based Gene Therapies: small packaging capacity	Bioprocessing Summit Europe (Barcelona, Spain)
2023/05/01	Lentiviral vector engineering: impact on manufacturing and vector performance,	American Society of Gene and Cell Therapy (ASGCT) (Los Angeles, United States)
2023/03/01	Expanding AAV vector Cargo Delivery	Bioprocessing Summit Europe Bioprocess International (Barcelona, Spain)
2022/03/07	Lentiviral Vector Design: Impact on Manufacturing and Vector Performance	Bioprocessing Summit Europe Bioprocess International (Barcelona, Spain)
2021	Challenges in Lentiviral Vector Development: From Manufacturing to Transduction of Target Cells	Bioprocessing Summit Europe (Virtual) Bioprocess International
2020	Enabling lentiviral vector based therapies: identifying barriers to high productivities	Upstream Process Development Strategies for Gene Therapies Bioprocessing Summit (Barcelona, Spain)
2019	Novel stable producer cell lines: enabling scalable lentiviral vector manufacturing	Gene Therapy – Ready for the Market DECHEMA Conferences (Frankfurt, Germany)
2019	Novel stable producer cells for the development of scalable lentiviral vector manufacturing processes	Cell and Gene Therapy Stream Bioprocessing Summit (Lisbon, Portugal)
2019	Enabling Gene and Cell Therapy: lentiviral vector & cell Engineering	26th meeting of European Society of Animal Cells European Society of Animal Cells (Copenhagen, Denmark)
2018	Novel stable lentiviral vector producer cells: overcoming viral vector cytotoxicity	Viral Vectors and Vaccines IsBiotech (Norfolk, United States)
2017	Virus like particles as scaffolds for vaccine development: a case study of Hepatitis C Virus	Viral Vectors and Vaccines IsBiotech (Whashington, United States)
2017	Enabling tools for gene therapy viral vector development	6th International Conference and Exhibition on Cell and Gene Therapy (Madrid, Spain)
2016	Challenges in Virus Producer Cell Line Development and Engineering: Novel Enabling Technologies Providing Solutions to Old Problems	Viral Vectors and Vaccines IsBiotech (Washington, United States)
2016	Development of HCV vaccine candidates using retroVLPs as manufacturing platform	10th Vaccines congress Elsevier (Amsterdan, Netherlands)
2015	Single step cloning-titration method: enabling tools for virus producer cell line development and engineering	Modern DNA concepts and tools for safe gene transfer and modification EMBO (Evry, France)
2015	Enabling tools for virus producer cell line development and engineering	24th meeting of European Society of Animal Cells European Society of Animal Cells (Barcelona, Spain)
2014	Enabling tools for Cell line development and Engineering: RMCE and screening methodologies.	Cell Line Development and Engineering Bioprocess International (Wien, Austria)
2014	Multi-Gene Engineering of Mammalian Cell Metabolism: new approaches and tools in the pursuit of hyperproductivity	XIV Cell Engineering ECI (Quebec, Canada)
2013	Single step cloning-titration method: enabling tools for virus producer cell line development and engineering	Process challenges for Viral Vaccines and Gene Therapy European Network on Viral Vaccine Processes - DECHEMA (Frankfurt, Germany)
2013	Single step cloning-titration method: accelerating the development and engineering of high-titer retro and lentiviral vector producer cell lines	21st meeting of European Society of Gene and Cell Therapy European Society of Gene and Cell Therapy (Madrid, Spain)
2012	Using functional genomics to unveil cell metabolic bottlenecks	Cell Line Development and Engineering Bioprocess Internatioal (Cologne, Germany)
2011	Unraveling Lipid Metabolism of Packaging Cells: Towards Improved Manufacture Of Retroviral Based Biopharmaceuticals	BioProcessing, Biologics & Biotherapeutics Congress BioProcessing, Biologics & Biotherapeutics (Edinburg, United Kingdom)
2011	Using functional genomics in the early stages of cell line development to preview production bottlenecks and product quality	Optimizing Cell Line Performance (London, United Kingdom)
2009	Site specific targeting and stoichiometric optimisation as means to engineer robust cell lines for manufacturing complex bioproducts	Cell Line Development and Engineering Bioprocess International (Berlin, Germany)
2009	Towards serum free production of retroviral vectors: Lipid supplementation strategies and manipulation of cells lipid metabolism	Clinigene Workshop in Gene and Cell Therapy Clinigene European Network of Excellence for Gene Therapy (Lucca, Italy)
2008	Flexible and stable cell lines for mammalian expression: cassette exchange system from simple to complex bioproducts	Cell Line Development and Engineering Bioprocess International (Prague, Czech Republic)
2007	Impact of Stoichiometry on the Productivity and Quality of Complex Bioproducts: the case of Retroviral Vectors	20th meeting of European Society of Animal Cells European Society of Animal Cells (Dresden, Germany)

Supervision

	Thesis Title Role	Degree Subject (Type) Institution / Organization
2021/04/05 - Current	Novel dual AAV vector platforms for large transgene delivery: enabling Duchenne Muscular Dystrophy gene therapies Supervisor of Mariana Ferreira	Ciências da Engenharia e Tecnologia (PhD) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2021/01/01 - Current	Novel packaging systems with abolished cytotoxicity: development of high-titer lentiviral vector producer cells Supervisor of Rodrigo Nogueira	Ciências da Engenharia e Tecnologia (PhD) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2020/01/01 - Current	HELV: high titer lentiviral vector producer cell lines based on replicative transposition Supervisor of Tiago André Gonçalves Vaz	Ciências da Engenharia e Tecnologia (PhD) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2022/10/01 - 2023/11/01	‘Development of a SARS-CoV-2 mimicking model for advancement of antiviral therapies’ Supervisor of Manuel Jacinto	Bioquímica (Master) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2019/01/01 - 2023/05/26	Accelerating Lentivirus Manufacturing Co-supervisor	Bioengenharia (PhD) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2019/10 - 2021/03/05	Live cell biosensors for detection of viral pathogens Supervisor of Ana Raquel Fernandes	Biochemistry for Health (Master) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2019/10/01 - 2021/01/21	Establishment of 293 based AAV packaging cell lines using shRNA-mediated rep silencing Supervisor of Joana Gaspar Diogo	Biotechnology (Master) Universidade de Lisboa Instituto Superior Técnico, Portugal
2016/04/01 - 2020/05/01	Improving gamma-retrovirus and lentivirus gene therapy vectors, from producer cell engineering to transduction performace Supervisor of Ana Sofia Formas Oliveira	Ciências da Engenharia e Tecnologia (PhD) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2017 - 2020	Development of quaternary aqueous biphasic systems for biomolecules separation and purification Co-supervisor of Mateusz Marechel	Bioengenharia (PhD) Universidade de Lisboa Instituto Superior Técnico, Portugal
2018/10/01 - 2019/11/30	Pseudotyped lentiviral vectors for gene therapy: impact of envelope glycoproteins Supervisor of RODRIGO JOSÉ GAMEIRO NOGUEIRA	Microbiologia Médica (Master) Universidade Nova de Lisboa Faculdade de Ciências Médicas, Portugal
2018/10/01 - 2019/11/01	Advanced cell-based biosensors for detection of label-free viral pathogens Supervisor of Ana Catarina Mandeiro da Silva	Biotechnology (Master) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2018/10/01 - 2019/11/01	Chimeric lentiviral vectors for gene therapy: improving transduction efficiency Supervisor of Elisa Thomé Cabral	Biochemistry for Health (Master) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2018/09/01 - 2019/11/01	Chimeric lentiviral vectors for gene therapy: characterization of stable producer cells Supervisor of Mariana Valentin Ferreira	Biotechnology (Master) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2012/04/01 - 2019/09/30	Cell-based switch-on sensors for detection and quantification of label-free viruses Supervisor of Miguel Ricardo Guerreiro	Ciências da Engenharia e Tecnologia (PhD) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2018 - 2019	Development of a baculovirus virion-free expression system Supervisor of Carmen Steffens	Medical Biotechnology (Master) Wageningen University and Research Research Institutes, Netherlands
2011 - 2019	Development of novel HIV-1 based lentiviral vector producer cells for gene therapy - The impact of mutated T26S HIV-1 protease in lentiviral vector production Supervisor of Hélio Antunes Tomás	Ciências da Engenharia e Tecnologia (PhD) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2017/10 - 2018/10	Novel fluorescent cell-based sensors for detection of viral pathogens Supervisor of Carolina Carreira	Molecular Genetics and Biomedicine (Master) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2017/10/01 - 2018	Genetic engineering of human cell lines for the improvement of viral vector production for gene therapy Supervisor of João Basílio	Biomedical Sciences (Master) Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2017 - 2018	Genetic engineering of mammalian cell lines for improved production of Gene Therapy Lentiviral vectors Supervisor of Victor van Gelder	Virology (Degree) Wageningen University & Research, Netherlands
2011 - 2018	Viral and cell hosts factors influencing the development of an HCV vaccine candidate Supervisor of Hugo Ricardo Loureiro Soares	Ciências da Engenharia e Tecnologia (PhD) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2016/10 - 2017/11	Novel Fluorescent Cell-Based Sensors for Detection of Viral Pathogens Supervisor of Daniela Freitas	Biomedical and Biophysical Engineering (Master) Universidade de Lisboa Faculdade de Ciências, Portugal
2016/10 - 2017/11	Genetic engineering of mammalian cell lines for improved production of gene therapy viral vectors Supervisor of Tiago André Gonçalves Vaz	Molecular Genetics and Biomedicine (Master) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2015/10 - 2016/11	A novel generation of lentiviral vectors for gene therapy Supervisor of Daniel Mestre	Biotechnology (Master) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2015/10 - 2016/10	Development of a VLP-based HCV vaccine candidate Supervisor of Marina Fernandes	Molecular Biology and Genetics (Master) Universidade de Lisboa Faculdade de Ciências, Portugal
2011 - 2015	Bioprocess design of canine adenovirus vectors for gene therapy applications Co-supervisor of Paulo David Machado dos Santos Fernandes	Ciências da Engenharia e Tecnologia (PhD) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2009 - 2014/01	Integrated functional genomics for improved manufacture of recombinante vírus: transcriptional profiling and metabolic engineering of retrovírus producer cells Supervisor of Ana Filipa de Albuquerque Ferreira Rodrigues	Ciências da Engenharia e Tecnologia (PhD) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2013 - 2014	Alternative cell substrates for high-titer lentiviral vector production Supervisor of Diogo Costa	Biomedical Engineering (Master) Universidade Católica Portuguesa, Portugal
2012 - 2013	Characterization of adherent and suspension MDCK cells as substrate for Canine Adenovirus Type 2 production Supervisor of Tanja Laske	Biosystems Engineering, (Master) Otto von Guericke Universität Magdeburg Fakultät für Verfahrens- und Systemtechnik, Germany
2011 - 2012	Application of functional genomics in the production of viral biopharmaceuticals: Vaccines and Vectors for gene therapy Supervisor of Ana Sofia Formas Oliveira	Cellular Biology and Biotecnology (Master) Universidade de Lisboa Faculdade de Ciências, Portugal
2011 - 2012	Produção de partículas semelhantes a retrovírus como candidatas a vacina para a hepatite C Supervisor of Ana Isabel Almeida	Molecular Genetics and Biomedicine (Master) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2008 - 2012	Understanding Human Carboxylesterase 2 and the feasibility of a more relevant cell model for intestinal metabolism Co-supervisor of Joana Catarina Rocha Lamego	Ciências da Engenharia e Tecnologia (PhD) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2010 - 2011	Expression of the human carboxylesterase 2 enzyme (CES2) in mammalian cells Co-supervisor of Bárbara Cunha	Biotechnology (Master) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2010 - 2011	Manipulação do efeito de Warburg em células de mamífero em cultura: implicações para o metabolismo energético Supervisor of Miguel Ricardo Guerreiro	Molecular Biology and Genetics (Master) Universidade de Lisboa Faculdade de Ciências, Portugal
2010 - 2010	Impacto da adaptação de células HEK293 a meio sem soro animal na produtividade de vectores retrovirais e particulas idênticas a retrovírus Supervisor of Joana Tavares	Biotechnology (Degree) Instituto Politécnico de Coimbra Escola Superior Agrária de Coimbra, Portugal
2009 - 2010	Development of an Affinity Pair tag-receptor for recombinant protein expression and purification Co-supervisor of Margarida Dias	Biotechnology (Master) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2009 - 2010	Desenvolvimento de linhas celulares produtoras de vectores retrovirais para terapia génica Supervisor of Hélio Tomás	Biotechnology (Master) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2006 - 2007	Influência do processo de transcrição reversa na perda da infecciosidade de vectores lentivirais Supervisor of João R. D. Dias	Molecular Biology and Genetics (Master) Universidade de Lisboa Faculdade de Ciências, Portugal
2006 - 2007	Efeito do soro na produção de vectores retrovirais para terapia génica: importância dos lipidos no meio de cultura Supervisor of Ana Filipa Rodrigues	Biochemistry (Degree) Universidade de Lisboa Faculdade de Ciências, Portugal

Event participation

	Activity description Type of event	Event name Institution / Organization
2007 - Current	Volunteer ‘Dia Aberto’ at ITQB-UNL – ITQB open day where the institute promotes the dissemination of science to the general public Exhibition	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2001 - Current	Over 150 poster presentation and regular attendances to international meetings in the areas of animal cell culture, gene therapy, vaccines and process development, biotechnology namely, Animal Cell Technology (ESACT), Cell Culture Engineering Congress (CCE), Cell Line Development and Engineering, European Society of Gene and Cell Therapy (ESGCT), American Society of Gene and Cell Therapy (ASCGT). Conference

Jury of academic degree

	Topic Role	Candidate name (Type of degree) Institution / Organization
2021/03/05	Live cell biosensors for detection and quantification of viral pathogens Supervisor	Ana Raquel Fernandes (Master) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2021/01/21	Establishment of 293 based AAV packaging cell lines using shRNA-mediated rep silencing Supervisor	Joana Gaspar Diogo (Master) Universidade de Lisboa Instituto Superior Técnico, Portugal
2020/05	Improving gamma-retrovirus and lentivirus gene therapy vectors, from producer cell engineering to transduction performace Supervisor	Ana Sofia Oliveira (PhD) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2019	Cell-based switch-on sensors for detection and quantification of label-free viruses Supervisor	Miguel Guerreiro (PhD) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2019	Development of novel HIV-1 based lentiviral vector producer cells for gene therapy - The impact of mutated T26S HIV-1 protease in lentiviral vector production Supervisor	Hélio Tomás (PhD) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2018	Viral and cell hosts factors influencing the development of an HCV vaccine candidate Supervisor	Hugo Soares (PhD) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2017	In vitro and in vivo models to assess cancer metastasis (Thesis) Main arguer	Constança Amaral (Master) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2015	Bioprocess design of canine adenovirus vectors for gene therapy applications Supervisor	Paulo Fernandes (PhD) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2014	Engineered structures for the profiling and enrichment of the phosphoproteome (Thesis) Arguer	Íris Cristina da Luz Batalha (PhD) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2014	Ana Filipa Rodrigues (2014) ‘Integrated functional genomics for improved manufacture of recombinant enveloped virus: transcriptional profiling and metabolic engineering of retrovirus producer cells’ Thesis Dissertation for obtaining the PhD degree in Technological and Engineering Sciences, Instituto de Tecnologia Química Biológica, Universidade Nova de Lisboa. Supervisor	Ana Filipa Rodrigues (PhD) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2013	Characterization of adherent and suspension MDCK cells as substrate for Canine Adenovirus Type 2 production (Thesis) Main arguer	Tanja Laske (Master) Otto von Guericke Universität Magdeburg Fakultät für Verfahrens- und Systemtechnik, Germany
2012	Understanding Human Carboxylase 2: and the feasibility of a more relevant model for intestinal metabolism Supervisor	Joana Lamego (PhD) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2008	Impact of a Newly Developed Human Derived Cell Line Upon Viral Vectors Production for Gene Therapy (Thesis) Arguer	Ana Carina Santos Ferreira da Silva (PhD) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2007	Adenovirus as a Biopharmaceutical: Optimisation of Vector Production (Thesis) Arguer	Tiago Bruno Pereira Soares Ferreira (PhD) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2006	Expression of Antibodies and Retroviral Vectors from Defined Chromosomal Sites: Strategies Towards Reliable Production Systems (Thesis) Arguer	Leonor da Gama Carvalho Norton (PhD) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Association member

	Society Organization name	Role
2016 - Current	American Society of Gene and Cell Therapy
2012 - Current	European Society of Gene and Cell Therapy
2006 - Current	European Society of Animal Cells (ESACT)	Member

Committee member

	Activity description Role	Institution / Organization
2013 - Current	Ph.D. Thesis committee member of João Vidigal, ITQB-UNL, Portugal Member	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2021/11/23 - 2021/11/26	Member of the organizing committee of Microbiotec 2021; Microbiotec congress is a biannual event since 2005 co-organized by the Portuguese Society of Microbiology and the Portuguese Society of Biotechnology. Member	Universidade Nova de Lisboa, Portugal
2015 - 2018	Ph.D thesis committee member of Hugo Almeida, ITQB-UNL, Portugal Member	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2014 - 2015	Member in the Scientific Council of ITQB-UNL Member	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2013 - 2015	Member of the organizing committee of Frontier Leaders of Today for the Scientists of Tomorrow; seminar series which brings renown researchers to Portugal in the areas of biology and chemistry. Member	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2010 - 2015	Ph.D. Thesis committee member Fabiana Fernandes, MIT-Portugal, Portugal Member	Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal

Conference scientific committee

	Conference name	Conference host
2023/01 - 2024/12/31	21st meeting of European Society of Gene and Cell Therapy	Manufacturing Session

Course / Discipline taught

	Academic session	Degree Subject (Type)	Institution / Organization
2022/10 - Current	Cell Culture-Based Viral Vaccines Course	(Postgraduate Certificate)	Europena Society for Animal Technology (ESACT), Ireland
2021/10 - Current	Bioprocessing and Manufacturing of Gene and Cell Therapy Products Course	Gene and Cell Therapy (Postgraduate Certificate)	Europena Society for Animal Technology (ESACT), Ireland
2020 - Current	Regenerative Medicine	Medicina (Licenciatura)	Universidade Nova de Lisboa Faculdade de Ciências Médicas, Portugal
2019 - Current	Medical Microbiology	Bioquímica para a Saúde (Mestrado)	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2013 - Current	Advanced therapy medicinal products	Biociências Moleculares (Doutoramento)	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2013 - Current	Biopharmaceuticals	Genética Molecular e Biomedicina (Mestrado integrado)	Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2010 - Current	Animal Cells Biotechnology	Biotecnologia (Mestrado)	Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2010 - Current	Industrial production and biotechnology products	Biotecnologia Farmacêutica (Mestrado integrado)	Universidade de Coimbra Faculdade de Farmácia, Portugal
2008 - Current	Biomolecular Methods	Biologia Molecular e Genética (Mestrado integrado)	Universidade de Lisboa Faculdade de Ciências, Portugal
2007 - Current	Frontiers in Biotechnology	Ciências e Engenharias Químicas e Biológicas (Doutoramento)	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2013 - 2018	Gene Therapy	Bioengenharia (Doutoramento)	Universidade de Lisboa Instituto Superior Técnico, Portugal
2008 - 2017	Biochemistry Engineering II	Ciências de Engenharia Química e Bioquímica (Licenciatura)	Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2013 - 2016	Synthetic and Systems Biology	Bioquímica para a Saúde (Mestrado)	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2006 - 2016	The use of Animal Cells in Biotechnology: an overview on cell culture	Cell Programming by nanoscaled devices (Doctor of Philosophy)	Universität Wien, Austria
2014 - 2014	Progress and Perspectives in Research	Genética Humana e Doenças Infecciosas (Doutoramento)	Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2008/06/01 - 2008/11/30	MIT Portugal	Bioengenharia (Doutoramento)	Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal

Evaluation committee

	Activity description Role	Funding entity
2014 - Current	Peer review panel member in calls for research projects: French National Research Agency (ANR) call for proposals in ‘Santé et Bien-Être Evaluator	Agence nationale de la recherche
2015 - 2017	Peer review panel member in calls for research projects: ERANet-LAC: Latin America, Caribbean and European Union’Infect-ERA Joint call for for proposals (Funded under the 7th research framework programme) Evaluator	European Commission
2014 - 2016	Peer review panel member in calls for research projects: PROJECTS : Infect-ERA Joint call for proposals (in the scope of the ERA-NET on Human Infectious Diseases Funded under the 7th research framework programme) Evaluator	European Commission

Mentoring / Tutoring

	Topic	Student name
2016 - 2016	Virology	Ana Lia Puglia
2015 - 2015	Hepatatis C vaccines	Sandra Patiño

Other jury / evaluation

	Activity description	Institution / Organization
2010 - Current	Editorial Member in Peer reviewed journals: - Scientific Reports - Biotechnology Letters - Advance in Genetic Engineering and Biotechnology, - The Scientific World Journal (Biotechnology panel) - Topic Editorial Board of Cells
2006 - Current	Reviewer in internacional scientific journals: Applied Microbiology and Biotechnology, Biochemical Engineering Journal, Biological Chemistry, Biotechnology and Bioengineering, BMC Biotechnology, Biotechnology Letters, OncoImmunology, Current Opinion in Structural Biology, Expert Opinion on Biological Therapy, Gene Therapy, Human Vaccines and Immunotherapeutics, Journal of Molecular Microbiology and Biotechnology, Journal of Virological Methods, Molecular Biotechnology, Molecular Therapy, Vaccine, Virus Research, among others.
2023/08/01 - 2023/10/10	Peer review Marie Sklodowska-Curie Postdoctoral Fellowships (MSCA-PF) (2023) European Research Executive Agency	European Research Executive Agency, Belgium