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I am currently serving as a junior researcher at ITQB-NOVA, having attained my Doctor of Philosophy (PhD) degree in the field of Biochemistry in the year 2013. My academic journey also encompasses a Bachelor's degree in Applied Chemistry, a Master of Science in Biotechnology, and an Integrated Master's degree in Medicine. Throughout my career, I have been involved in 15 research projects, with a primary emphasis on elucidating the intricate mechanisms underlying electron transfer within redox proteins. My contributions to the scientific community include the publication of 17 articles in peer-reviewed international journals, in addition to the authorship of 4 chapters in scientific books and 2 scientific preprints that are presently under revision in peer-reviewed journals (h-index of 9, Scopus, May 2024). My research findings have not remained confined to the written medium; they have been presented at more than 40 international and national conferences, underscoring my active engagement with the scientific discourse. Beyond my research, I have assumed organizational responsibilities for one scientific event and have been an enthusiastic participant in various community outreach initiatives. Furthermore, my role extends to the tutoring of students and laboratory management, a responsibility I currently hold. Additionally, I have successfully supervised a Master of Science dissertation and have undertaken the coordination of multiple graduate and undergraduate students in their academic pursuits.
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Identification

Personal identification

Full name
Bruno Miguel Oliveira Maia da Fonseca

Citation names

  • Fonseca, Bruno M

Author identifiers

Ciência ID
A515-6F04-3C40
ORCID iD
0000-0003-1364-5871
Google Scholar ID
https://scholar.google.com/citations?user=hoWpn3oAAAAJ&hl=en
Scopus Author Id
14033914800

Email addresses

  • bfonseca@itqb.unl.pt (Professional)

Telephones

Telephone
  • 214469333 (Professional)

Addresses

  • Instituto de Tecnologia Química e Biológica - Universidade Nova de Lisboa. ITQB- Av. da República (EAN), 2781-901, Oeiras, Oeiras, Portugal (Professional)

Websites

Knowledge fields

  • Natural sciences - Biological Sciences - Biochemistry
  • Natural sciences - Biological Sciences - Microbiology
  • Natural sciences - Biological Sciences - Molecular Biology
  • Natural sciences - Biological Sciences - Biochemical Research Methods
  • Medical and Health Sciences - Health Sciences
  • Medical and Health Sciences - Basic Medicine
  • Medical and Health Sciences - Clinical Medicine

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese (Mother tongue)
English Proficiency (C2) Proficiency (C2) Proficiency (C2) Proficiency (C2)
French Beginner (A1) Beginner (A1) Beginner (A1) Beginner (A1)
Spanish; Castilian Beginner (A1) Beginner (A1) Beginner (A1) Beginner (A1)
Education
Degree Classification
2020/06/19
Concluded
 Master's in Medicine (Mestrado integrado)
Universidade de Lisboa Faculdade de Medicina, Portugal
 15 (0-20)
2013/05/03
Concluded
 ITQB PhD program - Biochemistry (Doutoramento)
Major in Biochemistry - Bioenergétics
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
"Mind the gap: characterization of periplasmic cytochromes from Shewanella oneidensis involved in extracellular electron transfer" (THESIS/DISSERTATION)
 Unanimously approved
2007/11/26
Concluded
 Master's in Biotecnology (Mestrado)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
"Preliminary studies of terminal reductases from the respiratory chains of Desulfuromonas acetoxidans" (THESIS/DISSERTATION)
 17 (0-20)
2006/12/07
Concluded
 Licentiate in Applied Chemistry (Licenciatura)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
 15 (0-20)
Affiliation

Science

Category
Host institution 		Employer
2018/11/01 - Current Contracted Researcher (Research) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2014/01/01 - 2018/10/31 Postdoc (Research) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Others

Category
Host institution 		Employer
2023/10/23 - 2023/10/28 Research training in Protein computational modelling Universidad de Sevilla, Spain
Institute for Chemical Research (IIQ) - Centre of Scientific Research Isla de la Cartuja (cicCartuja) , Spain
2013/08/01 - 2013/12/31 Research fellow (Felowship Ref. 069/BI-BI/2013) in the FCT project (Ref. PTDC/QUI-BIQ/117440/2010) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2008/01/01 - 2013/05/03 PhD student (Fellowship Ref. SFRH/BD/41205/2007 of the Fundação para a Ciência e a Tecnologia) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2011/08/01 - 2011/12/31 Internship in Fragment-based drug discovery at the Molecular Interactions & Biophysics Laboratory Merck KGaA, Germany
2010/06/25 - 2010/07/25 Research training in Microbiology Stanford University, United States
2010/03/01 - 2010/04/30 Research training in Molecular Biology Pacific Northwest National Laboratory, United States
2007/01/01 - 2007/12/31 BI student (Project Ref. POCI/BIO/58652/2004 of the Fundação para a Ciência e a Tecnologia) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2005/06/01 - 2006/12/31 Undergraduate Research Trainee Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2004/03/01 - 2004/07/30 Undergraduate Research Trainee Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
Projects

Grant

Designation Funders
2022/01/01 - Current The power of salt: using the marine bacterium Desulfuromonas acetoxidans for electricity production and water desalination
PTDC/BIA-BQM/4143/2021
Researcher
Fundação para a Ciência e a Tecnologia
Ongoing
2022/09/19 - 2026/09/18 Iron-sulphur (FeS) clusters: from chemistry to immunology (FeSImmChemNet)
CA21115
Researcher
Horizon 2020
Ongoing
2018/10/01 - 2021/09/30 C-genesis: Determination of the molecular mechanisms of biogenesis of c-type cytochromes
PTDC/BIA-BQM/30176/2017
Researcher
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2018 - 2021 TIMB3: Twin to Illuminate Metals in Biology and Biocatalysis through Biospectroscopy
H2020-WIDESPREAD grant agreement no. 810856
Researcher
Horizon 2020
Concluded
2016/05/01 - 2019/10/31 The difference a cell wall makes: optimization of bioelectrochemical systems by exploring the paradigm of extracellular electron transfer in Gram-positive bacteria
PTDC/BBB-BQB/4178/2014
Researcher
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Associação para a Inovação e Desenvolvimento da FCT, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2016 - 2019 The way forward: optimization of respiratory electron transfer chains toward sustainable microbial electricity production
PTDC/BBB-BQB/3554/2014
Researcher
Fundação para a Ciência e a Tecnologia
Concluded
2014/01/01 - 2018/10/31 Emerging human pathogens from the Shewanella genus: understanding the molecular mechanism behind ferric iron-siderophore reduction
SFRH/BPD/93164/2013
Post-doc Fellow
Fundação para a Ciência e a Tecnologia
Concluded
2012 - 2015 Redox necklaces: functional characterization of a multidomain polyheme cytochrome
PTDC/QUI-BIQ/117440/2010
Research Fellow
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2012 - 2015 Molecular mechanisms that orchestrate a two-electron reduction step coupled with protonation in redox enzymes that contain chains of single electron redox co-factors
PTDC/BIA-PRO/117523/2010
Research Fellow
Fundação para a Ciência e a Tecnologia
Concluded
2010/02/01 - 2013/02/28 Mind the gap: How extracellular respiration is linked across the periplasmic space to the cytoplasmic oxidation of substrates. A key step in bioenergy harvesting.
PTDC/BIA-PRO/098158/2008
PhD Student Fellow
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2008 - 2012 Mind the gap: characterization of periplasmic cytochromes from Shewanella oneidensis involved in extracellular electron transfer
SFRH/BD/41205/2007
PhD Student Fellow
Fundação para a Ciência e a Tecnologia
Concluded
2006/01/01 - 2007/12/31 Characterization of the respiratory chains of sulfur and metals from a marine microorganism identified for use in bioremediation
POCI/BIO/58652/2004
Research Fellow
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded

Contract

Designation Funders
2023/09/01 - Current Life Sciences for a Healthy and Sustainable Future
LA/P/0087/2020
10.54499/LA/P/0087/2020
Researcher
Instituto Português de Oncologia de Lisboa Francisco Gentil EPE, Portugal

Universidade Nova de Lisboa Molecular Structural and Cellular Microbiology, Portugal

Universidade Nova de Lisboa, Portugal

Instituto de Biologia Experimental e Tecnológica, Portugal

Instituto Gulbenkian de Ciência, Portugal

Universidade de Lisboa Centro de Estudos Comparatistas, Portugal

BioResources 4 Sustainability, Portugal

Universidade de Trás-os-Montes e Alto Douro Centro de Investigação e de Tecnologias Agro-Ambientais e Biologicas, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2019/01/01 - 2019/12/31 Molecular, Structural and Cellular Microbiology
UID/CBQ/04612/2019
Researcher
Universidade Nova de Lisboa Molecular Structural and Cellular Microbiology, Portugal

Universidade Nova de Lisboa, Portugal

Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica António Xavier, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2011/01/01 - 2012/12/31 Strategic Project - LA 4 - 2011-2012
PEst-OE/EQB/LA0004/2011
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica António Xavier, Portugal

Fundação Calouste Gulbenkian, Portugal

Instituto de Biologia Experimental e Tecnológica, Portugal

Universidade NOVA de Lisboa NOVA Medical School, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
Outputs

Publications

Book chapter
  1. Fonseca, Bruno M; M. Paquete, Catarina; Louro, Ricardo. "Bacterial power: An alternative energy source". In Enzymes for Solving Humankind's Problems. Springer, 2020.
    Published
  2. Louro, R.O.; Costa, N.L.; Fernandes, A.P.; Silva, A.V.; Trindade, I.B.; Fonseca, B.M.; Paquete, C.M.. "Exploring the molecular mechanisms of extracellular electron transfer for harnessing reducing power in METs: Methodologies and approaches". In Microbial Electro-chemical Technology: Platform for Fuels, Chemicals and Remediation, 261-293. Elsevier, 2019.
    Published
  3. Fonseca, Bruno; M. Paquete, Catarina; Louro, Ricardo. "Multi-electron transfer in biological systems". In Redox Proteins in Supercomplexes and Signalosomes. CRC Press, 2015.
    Published
  4. Fonseca, Bruno M; Alves, Alexandra S.; Louro, Ricardo. "Sludge oomph: harnessing the power of sediment microbiota". In Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology. Badajoz, Spain: Formatex Research Center, 2010.
    Published
Conference abstract
  1. Estelle Lebègue; Nazua L Costa; Bruno M Fonseca; Ricardo O Louro; Frédéric Barrière. "Electrochemical Properties of pH-Dependent Flavocytochrome C 3 from Shewanella Putrefaciens Adsorbed Onto Catechol-Modified Carbon Electrode". Paper presented in ECS Meeting, 2020.
    10.1149/MA2020-01442522mtgabs
  2. Fonseca, Bruno M; M. Paquete, Catarina; Neto, Sónia E; Cruz, David; Pacheco, Isabel; Soares, Claudio M.; Louro, Ricardo. "Molecular details of indirect extracellular electron transfer between bacteria and metallic solids or electrodes". 2014.
    Published
  3. Neto, Sónia E; Fonseca, Bruno M; Shi, Liang; Rivera, Mário; Tien, Ming; Maycock, Christopher; Louro, Ricardo. "Labeling of hemes for structural studies of cytochromes". 2012.
    Published
  4. Louro, Ricardo; M. Paquete, Catarina; Saraiva, Ivo H; Fonseca, Bruno M; Alves, Alexandra S; Pacheco, Isabel. "Mind the gap: characterization of proteins responsible for driving electrons across the microbe electrode interface". 2012.
    Published
Conference poster
  1. Trindade, Inês B.; Fonseca, Bruno M; Catarino, Teresa; Matias, Pedro M; Moe, Elin; Louro, Ricardo. "Flavin-containing siderophore-interacting protein of Shewanella putrefaciens DSM 9451 reveals substrate specificity in ferric-siderophore reduction". Paper presented in 17th European Biological Inorganic Chemistry Conference (EuroBIC-17), 2024.
  2. Soares, Ricardo; Fonseca, Bruno M; Nash, Benjamin W; M. Paquete, Catarina; Louro, Ricardo. "Pangenomic analysis of multiheme c- type cytochromes from the Desulfuromonadia class". Paper presented in 17th European Biological Inorganic Chemistry Conference (EuroBIC-17), 2024.
  3. Bayar, Büsra; Trindade, Inês B.; Alves, Alexandra S.; Fonseca, Bruno M; Maria O. Firmino; M. Paquete, Catarina; Louro, Ricardo O.. "Teaching an old dog new tricks: Investigation and implementation of bidirectional extracellular electron transfer in Rhodopseudomonas palustris". Paper presented in 4th International Meeting Biochemical Foundation of Microbial Extracellular Electron Transfer, 2024.
  4. Maria O. Firmino; Trindade, Inês B.; Fonseca, Bruno M; Moe, Elin; Matias, Pedro M; Louro, Ricardo. "Comparative Analysis of Flavocytochrome c Redox Properties in Pathogenic and Non-pathogenic Shewanella Species". Paper presented in 48th FEBS Congress – Mining biochemistry for human health and well-being, 2024.
  5. Fonseca, Bruno M; Tamargo-Azpilicueta, Joaquín; M. Paquete, Catarina; Díaz-Moreno, Irene; Louro, Ricardo O.. "Methyl Orange: Use it at your own risk". Paper presented in XXVIII International Symposium on Bioelectrochemistry and Bioenergetics of the Bioelectrochemical Society, 2024.
  6. Soares, Ricardo; Fonseca, Bruno M; Nash, Benjamin W; M. Paquete, Catarina; Louro, Ricardo. "Known unknowns, unknown unknowns and unknown knowns: A brief report from the survey of the Desulfuromonadia “cytochromome"". Paper presented in 14th Inorganic & Bioinorganic Chemistry Conference IBiCC, 2024.
  7. Fonseca, Bruno M; Tamargo-Azpilicueta, Joaquín; M. Paquete, Catarina; Díaz-Moreno, Irene; Louro, Ricardo. "Deciphering azo dye binding: Probing exoelectrogenic cytochrome-mediated bioremediation pathways". Paper presented in 14th Inorganic & Bioinorganic Chemistry Conference IBiCC, 2024.
  8. Soares, Ricardo; Fonseca, Bruno M; Nash, Benjamin W; M. Paquete, Catarina; Louro, Ricardo O.. "A survey of the Desulfuromonadia cytochromome provides a glimpse of the unexplored diversity of multiheme cytochromes in nature". Paper presented in Gordon Research Conference - Metals in Biology, 2024.
  9. Maria O. Firmino; Trindade, Inês B.; Fonseca, Bruno M; Moe, Elin; Matias, Pedro M; Louro, Ricardo. "Is the flavocytochrome c from pathogenic Shewanella different from those of other Shewanella species?". Paper presented in TIMB3 Final Meeting, 2022.
  10. Alves, Alexandra S.; Trindade, Inês B.; Maria O. Firmino; Noordam, Sander; Fonseca, Bruno M; M. Paquete, Catarina; Louro, Ricardo. "An insight into how extracellular electron uptake occurs in Rhodopseudomonas palustris TIE-1". Paper presented in TIMB3 Final Meeting, 2022.
  11. Louro, R.O.; Paquete, C.M.; Tien, M.; Matias, P.M.; Moe, E.; Costa, N.; Silva, L.; et al. "Manipulation of the electron transfer properties of multiheme cytochromes and their impact in extracellular electron transfer metabolism". Paper presented in Electromicrobiology 2021, 2021.
  12. Paquete, C.M.; Faustino, M.; Costa, N.L.; Fonseca, B.M.; Lousa, D.; Louro, R.O.. "Crossing the cell-wall in Gram-positive bacteria: understanding the extracellular electron transfer processes of Thermincola sp.". Paper presented in Electromicrobiology 2021, 2021.
  13. Trindade, Inês B.; Didier, Inês; Fonseca, Bruno M; Silva, José M.; Catarino, Teresa; Matias, Pedro M; Moe, Elin; Louro, Ricardo. "Metal uptake in Shewanella: exploring the mechanisms of siderophore recycling". Paper presented in ISMET 7, 2019.
  14. Fonseca, Bruno M; Silva, Luis; Trindade, Inês B.; Moe, Elin; Matias, Pedro M; Louro, Ricardo; M. Paquete, Catarina. "Optimizing electroactive organisms: the effect of orthologous proteins". Paper presented in ISMET 7, 2019.
  15. Trindade, Inês B.; Invernici, M; Costa, Nazua L.; Saraiva, Ivo H; Fonseca, Bruno M; Piccioli M; Cantini F; Louro, Ricardo. "Determining the NMR structure of PioC: the role of metalloproteins in photoferrotrophic iron oxidation". Paper presented in TIMB3 Annual Meeting, 2019.
  16. Trindade, Inês B.; Fonseca, Bruno M; Louro, Ricardo. "Shining light on photoferrotrophic iron oxidation: the structure & function of metalloproteins in Rhodopseudomonas palustris TIE-1". Paper presented in ICBIC 19, 2019.
  17. M. Paquete, Catarina; Fonseca, Bruno M; Silva, Luis; Trindade, Inês B.; Moe, Elin; Matias, Pedro M; Louro, Ricardo. "Biological electron transfer processes: the effect of orthologous proteins". Paper presented in Research conference in Cell Biology of Metals: Organismal Biology of Metals and Related Metal Complexes, 2019.
  18. Trindade, Inês B.; Silva, José M.; Fonseca, Bruno M; Catarino, Teresa; Fujita, Masaki; Matias, Pedro M; Moe, Elin; Louro, Ricardo. "Unraveling the mechanisms of ferric-siderophore reduction: The unforeseen versatility of SfSIP, a Siderophore-Interacting Protein from Shewanella frigidimarina". Paper presented in Gordon Research conference in Cell Biology of Metals: Organismal Biology of Metals and Related Metal Complexes, 2019.
  19. Trindade, Inês B.; Fonseca, Bruno M; Moe, Elin; Matias, Pedro M; M. Paquete, Catarina; Louro, Ricardo. "Crystalizing iron pathways: Structure and reactivity of metalloproteins". Paper presented in IACT (CSIC-UGR), 2019.
  20. Soares, Ricardo; Alves, Alexandra S.; Fonseca, Bruno M; M. Paquete, Catarina; Louro, Ricardo. "Expression and purification of multiheme cytochromes involved in the extracellular respiration of the marine bacterium Desulfuromonas acetoxidans". Paper presented in TIMB3 meeting, 2018.
  21. Trindade, Inês B.; Silva, José M.; Fonseca, Bruno M; Catarino, Teresa; Fujita, Masaki; Matias, Pedro M.; Moe, Elin; Louro, Ricardo. "Unforeseen versatility in ferric-siderophore reduction: the structural & functional characterization of SIP, a Siderophore-Interacting protein from Shewanella frigidimarina". Paper presented in EuroBIC14, 2018.
  22. Trindade, Inês B.; Fonseca, Bruno M; Silva, José M.; Catarino, Teresa; Matias, Pedro M; Moe, Elin; Louro, Ricardo. "Exploring the molecular mechanisms of siderophore recycling in Shewanella". Paper presented in ISMET6, 2017.
  23. Trindade, Inês B.; Fonseca, Bruno M; Silva, José M.; Catarino, Teresa; Matias, Pedro M; Moe, Elin; Louro, Ricardo. "The mysteries of microbial iron piracy: A siderophore interacting protein from Shewanella frigidimarina". Paper presented in FEBS Practical and Lecture Course - Chemistry of metals in biological systems, 2017.
  24. Silva, José M.; Trindade, Inês B.; Fonseca, Bruno M; Matias, Pedro M; Louro, Ricardo; Moe, Elin. "Crystal structural determination of a putative siderophore interacting protein from the marine bacterium Shewanella frigidimarina". Paper presented in 6th National Meeting of Portuguese Synchrotron Radiation Users (ENURS), 2017.
  25. Trindade, Inês B.; Fonseca, Bruno M; Matias, Pedro M; Louro, Ricardo; Moe, Elin. "Marine iron scavenging: a siderophore interacting protein from the marine bacterium Shewanella frigidimarina". Paper presented in ENURS2016, 2016.
  26. Fonseca, Bruno M; Trindade, Inês B.; Louro, Ricardo. "Understanding the molecular mechanism behind ferric iron-siderophore reduction in the marine bacterium Shewanella frigidimarina". Paper presented in IX Reunión Temática de la Red de Estructura y Función de Proteínas, 2015.
  27. Trindade, Inês B.; Fonseca, Bruno M; Louro, Ricardo. "Expression and preliminar characterization of a ferric siderophore reductase from a novel human pathogen Shewanella algae". Paper presented in IX Reunión Temática de la Red de Estructura y Función de Proteínas, 2015.
  28. Fonseca, Bruno M; M. Paquete, Catarina; Louro, Ricardo. "Emerging human pathogens from the Shewanella genus: understanding the molecular mechanism behind ferric iron-siderophore reduction". Paper presented in 7th Young Scientist Symposium, 2014.
  29. M. Paquete, Catarina; Fonseca, Bruno M; Cruz, David E; Pereira, Tiago; Pacheco, Isabel; Soares, Claudio M.; Louro, Ricardo. "Exploring the molecular interactions responsible for indirect electron transfer in Shewanella oneidensis MR-1". Paper presented in 2nd European International Society for Microbial Electrochemistry and Technology Meeting, 2014.
  30. Fonseca, Bruno M; M. Paquete, Catarina; Neto, Sónia E; Pacheco, Isabel; Soares, Claudio M.; Louro, Ricardo. "Mind the Gap: Cytochrome interactions reveal electron pathways across the periplasm of Shewanella oneidensis MR-1". Paper presented in Gordon Research Conference, 2013.
  31. Neto, Sónia E; Fonseca, Bruno M; Shi, Liang; Rivera, Mário; Tien, Ming; Maycock, Christopher; Louro, Ricardo. "Labeling of hemes for structural studies of cytochromes". Paper presented in 22nd IUBMB & 37th FEBS Congress, 2012.
  32. Neto, Sónia E; Fonseca, Bruno M; Maycock, Christopher; Louro, Ricardo. "Biosynthesis of labeled hemes for structural studies of cytochromes". Paper presented in 2nd PTNMR Meeting, 2012.
  33. Fonseca, Bruno M; Tien, Ming; Rivera, Mário; Shi, Liang; Louro, Ricardo. "A novel strategy to obtain specific isotopic labeling of multiheme cytochromes c: applications to the extracellular respiratory metabolism of Shewanella oneidensis MR-1". Paper presented in Bioinorganic Chemistry Gordon Research Seminar, 2012.
  34. Fonseca, Bruno M; M. Paquete, Catarina; Shi, Liang; Louro, Ricardo. "A key step for the optimization of Microbial Fuel Cells: Characterization of multiheme cytochromes from Shewanella oneidensis MR-1". Paper presented in E3 Forum, 2011.
  35. Fonseca, Bruno M; M. Paquete, Catarina; Alves, Alexandra S.; Louro, Ricardo. "Characterization of multiheme cytochromes from Shewanella oneidensis MR-1: A key step for the optimization of Microbial Fuel Cells". Paper presented in 3rd International MFC Conference, 2011.
  36. Fonseca, Bruno M; M. Paquete, Catarina; Alves, Alexandra S.; Louro, Ricardo. "Characterization of multiheme cytochromes from Shewanella oneidensis MR-1". Paper presented in FEBS combined practical and lecture course Chemistry of Metals in Biological Systems, 2011.
  37. Alves, Alexandra S.; Fonseca, Bruno M; M. Paquete, Catarina; Louro, Ricardo. "Studies on extracellular respiration in sediment bacteria". Paper presented in 1st ITQB PhD student meeting, 2010.
  38. Alves, Alexandra S; Fonseca, Bruno M; Louro, Ricardo. "Terminal Reductases of Anaerobic Sulfur Respiring Organisms". Paper presented in EMBO-FEBS Workshop on Microbial Sulfur Metabolism, 2009.
  39. Maria Alexandra dos Santos Alves; Fonseca, Bruno M; Saraiva, Ivo H; Calçada, Eduardo; M. Paquete, Catarina; Louro, Ricardo. "Characterization of electron transfer chains for the respiration of insoluble substrates". Paper presented in EMBO-FEMS workshop on microbial sulfur metabolism, 2009.
  40. M. Paquete, Catarina; Fonseca, Bruno M; Saraiva, Ivo H; Alves, Alexandra S.; Louro, Ricardo. "Mind the gap: Using NMR to explore the redox chain of metalloproteins implicated in extracellular respiration". Paper presented in Magnetic Resonance in the life sciences: what's new, 2008.
  41. Fonseca, Bruno M; Saraiva, Ivo H; M. Paquete, Catarina; Soares, Claudio M.; Pacheco, Isabel; Salgueiro, Carlos A.; Louro, Ricardo. "Thermodynamic bias for functional specificity of the different hemes of the small tetraheme cytochrome c from Shewanella oneidensis". Paper presented in IV Bienal of NMR, 2008.
  42. Fonseca, Bruno M; Saraiva, Ivo H; Pacheco, Isabel; M. Paquete, Catarina; Louro, Ricardo. "Importance of the Redox-Bohr effect for the diversity in functionality between soluble small tetraheme cytochromes from Shewanella sp.". Paper presented in Biometals Conference, 2008.
  43. Fonseca, Bruno M; Saraiva, Ivo H; Pacheco, Isabel; M. Paquete, Catarina; Louro, Ricardo. "The redox-Bohr effect modulates the functional properties of tetraheme cytochromes from Shewanella sp.". Paper presented in BioMetals 2008: 6th International Biometals Symposium, 2008.
  44. Fonseca, Bruno M; Saraiva, Ivo H; Pacheco, Isabel; M. Paquete, Catarina; Louro, Ricardo. "Thermodynamic characterization of the small tetraheme cytochrome from Shewanella oneidensis MR-1: coupling of electron and proton transfer in the physiological pH". Paper presented in Gordon Research Conference - Metals in Biology, 2008.
  45. Fonseca, Bruno M; Saraiva, Ivo H; Pacheco, Isabel; M. Paquete, Catarina; Louro, Ricardo. "Redox-Bohr effect in the soluble small tetraheme cytochrome c from Shewanella oneidensis MR-1". Paper presented in Euromar - Magnetic Resonance Conference, 2007.
  46. Fonseca, Bruno M; Rosa, Andre; Ramos, Paulo. "Cyclic voltammetry study of the antibiotic Chloramphenicol". Paper presented in IV Forum da Química, 2006.
  47. Fonseca, Bruno M; Pereira, Patricia; Pacheco, Isabel; Louro, Ricardo. "Preliminary study of soluble heme proteins from Shewanella oneidensis MR-1". Paper presented in III European Meeting on Oxizymes, 2006.
  48. Salgueiro, Carlos A.; Morgado, Leonor; Fonseca, Bruno M; Lamosa, Pedro; Catarino, Teresa; Turner, David L; Louro, Ricardo. "Thermodynamic properties of the bound form of cytochrome c3". Paper presented in Metals in Biology Gordon Research Conference, 2005.
Journal article
  1. Trindade, Inês B.; Firmino, Maria O.; Noordam, Sander J.; Alves, Alexandra S.; Fonseca, Bruno M.; Piccioli, Mario; Louro, Ricardo O.. "Protein Interactions in Rhodopseudomonas palustris TIE-1 Reveal the Molecular Basis for Resilient Photoferrotrophic Iron Oxidation". Molecules 28 12 (2023): 4733. http://dx.doi.org/10.3390/molecules28124733.
    Published • 10.3390/molecules28124733
  2. Louro, Ricardo O.; Rusconi, Giovanni; Fonseca, Bruno M.; Paquete, Catarina M.. "Deciphering Molecular Factors That Affect Electron Transfer at the Cell Surface of Electroactive Bacteria: The Case of OmcA from Shewanella oneidensis MR-1". Microorganisms 11 1 (2022): 79. http://dx.doi.org/10.3390/microorganisms11010079.
    Published
  3. Faustino, Marisa M.; Fonseca, Bruno M.; Costa, Nazua L.; Lousa, Diana; Louro, Ricardo O.; Paquete, Catarina M.. "Crossing the Wall: Characterization of the Multiheme Cytochromes Involved in the Extracellular Electron Transfer Pathway of Thermincola ferriacetica". Microorganisms 9 2 (2021): 293. http://dx.doi.org/10.3390/microorganisms9020293.
    Open access • Published • 10.3390/microorganisms9020293
  4. Fonseca, Bruno M.; Silva, Luis; Trindade, Ines B.; Moe, Elin; Matias, Pedro M.; Louro, Ricardo O.; Paquete, Catarina M.. "Optimizing Electroactive Organisms: The Effect of Orthologous Proteins". Frontiers in Energy Research (2019): http://hdl.handle.net/10362/85355.
    Published • https://doi.org/10.3389/fenrg.2019.00002
  5. Lebègue, Estelle; Costa, Nazua L.; Fonseca, Bruno M.; Louro, Ricardo O.; Barrière, Frédéric. "Electrochemical properties of pH-dependent flavocytochrome c3 from Shewanella putrefaciens adsorbed onto unmodified and catechol-modified edge plane pyrolytic graphite electrode". Journal of Electroanalytical Chemistry (2019): http://hdl.handle.net/10362/91638.
    Published • https://doi.org/10.1016/j.jelechem.2019.113232
  6. Trindade, Inês B.; Silva, José M.; Fonseca, Bruno M.; Catarino, Teresa; Fujita, Masaki; Matias, Pedro M.; Moe, Elin; Louro, Ricardo O.. "Structure and reactivity of a siderophore-interacting protein from the marine bacterium Shewanella reveals unanticipated functional versatility". Journal of Biological Chemistry (2019): http://www.scopus.com/inward/record.url?scp=85059495865&partnerID=8YFLogxK.
    Published • https://doi.org/10.1074/jbc.RA118.005041
  7. Trindade, I.B.; Fonseca, B.M.; Matias, P.M.; Louro, R.O.; Moe, E.. "A putative siderophore-interacting protein from the marine bacterium Shewanella frigidimarina NCIMB 400: Cloning, expression, purification, crystallization and X-ray diffraction analysis". Acta Crystallographica Section:F Structural Biology Communications 72 (2016): 667-671. http://www.scopus.com/inward/record.url?eid=2-s2.0-84986325474&partnerID=MN8TOARS.
    Published • 10.1107/S2053230X16011419
  8. Alves, M.N.; Neto, S.E.; Alves, A.S.; Fonseca, B.M.; Carrêlo, A.; Pacheco, I.; Paquete, C.M.; Soares, C.M.; Louro, R.O.. "Characterization of the periplasmic redox network that sustains the versatile anaerobic metabolism of Shewanella oneidensis MR-1". Frontiers in Microbiology 6 JUN (2015): http://www.scopus.com/inward/record.url?eid=2-s2.0-84937144196&partnerID=MN8TOARS.
    Published • 10.3389/fmicb.2015.00665
  9. Neto, S.E.; Fonseca, B.M.; Maycock, C.; Louro, R.O.. "Analysis of the residual alignment of a paramagnetic multiheme cytochrome by NMR". Chemical Communications 50 35 (2014): 4561-4563. http://www.scopus.com/inward/record.url?eid=2-s2.0-84898749407&partnerID=MN8TOARS.
    Published • 10.1039/c3cc49135h
  10. Paquete, C.M.; Fonseca, B.M.; Cruz, D.R.; Pereira, T.M.; Pacheco, I.; Soares, C.M.; Louro, R.O.. "Exploring the molecular mechanisms of electron shuttling across the microbe/metal space". Frontiers in Microbiology 5 JUN (2014): http://www.scopus.com/inward/record.url?eid=2-s2.0-84904907260&partnerID=MN8TOARS.
    Published • 10.3389/fmicb.2014.00318
  11. Fonseca, B.M.; Paquete, C.M.; Louro, R.O.. "Molecular mechanisms of heme based sensors from sediment organisms capable of extracellular electron transfer". Journal of Inorganic Biochemistry 133 (2014): 104-109. http://www.scopus.com/inward/record.url?eid=2-s2.0-84897116183&partnerID=MN8TOARS.
    Published • 10.1016/j.jinorgbio.2013.10.024
  12. Fonseca, B.M.; Paquete, C.M.; Neto, S.E.; Pacheco, I.; Soares, C.M.; Louro, R.O.. "Mind the gap: Cytochrome interactions reveal electron pathways across the periplasm of Shewanella oneidensis MR-1". Biochemical Journal 449 1 (2013): 101-108. http://www.scopus.com/inward/record.url?eid=2-s2.0-84870912011&partnerID=MN8TOARS.
    Published • 10.1042/BJ20121467
  13. Fonseca, B.M.; Tien, M.; Rivera, M.; Shi, L.; Louro, R.O.. "Efficient and selective isotopic labeling of hemes to facilitate the study of multiheme proteins". BioTechniques 52 4 (2012): http://www.scopus.com/inward/record.url?eid=2-s2.0-84973431205&partnerID=MN8TOARS.
    Published • 10.2144/000113859
  14. Fonseca, B.M.; Paquete, C.M.; Salgueiro, C.A.; Louro, R.O.. "The role of intramolecular interactions in the functional control of multiheme cytochromes c". FEBS Letters 586 5 (2012): 504-509. http://www.scopus.com/inward/record.url?eid=2-s2.0-84857911836&partnerID=MN8TOARS.
    Published • 10.1016/j.febslet.2011.08.019
  15. Alves, A.S.; Paquete, C.M.; Fonseca, B.M.; Louro, R.O.. "Exploration of the 'cytochromome' of Desulfuromonas acetoxidans, a marine bacterium capable of powering microbial fuel cells". Metallomics 3 4 (2011): 349-353. http://www.scopus.com/inward/record.url?eid=2-s2.0-79953777998&partnerID=MN8TOARS.
    Published • 10.1039/c0mt00084a
  16. Fonseca, B.M.; Saraiva, I.H.; Paquete, C.M.; Soares, C.M.; Pacheco, I.; Salgueiro, C.A.; Louro, R.O.. "The tetraheme cytochrome from Shewanella oneidensis MR-1 shows thermodynamic bias for functional specificity of the hemes". Journal of Biological Inorganic Chemistry 14 3 (2009): 375-385. http://www.scopus.com/inward/record.url?eid=2-s2.0-61849083078&partnerID=MN8TOARS.
    Published • 10.1007/s00775-008-0455-7
  17. Salgueiro, C.A.; Morgado, L.; Fonseca, B.; Lamosa, P.; Catarino, T.; Turner, D.L.; Louro, R.O.. "Binding of ligands originates small perturbations on the microscopic thermodynamic properties of a multicentre redox protein". FEBS Journal 272 9 (2005): 2251-2260. http://www.scopus.com/inward/record.url?eid=2-s2.0-18444376154&partnerID=MN8TOARS.
    Published • 10.1111/j.1742-4658.2005.04649.x
Preprint
  1. Soares, Ricardo; Fonseca, Bruno M; Nash, Benjamin W; M. Paquete, Catarina; Louro, Ricardo. "A Survey of the Desulfuromonadia cytochromome provides a glimpse of the unexplored diversity of multiheme cytochromes in nature". 2024.
    https://doi.org/10.1101/2024.01.05.574438
  2. Trindade, Inês B.; Fonseca, Bruno M; Catarino, Teresa; Matias, Pedro M.; Moe, Elin; Louro, Ricardo O.. "Flavin-containing siderophore-interacting protein of Shewanella putrefaciens DSM 9451 reveals substrate specificity in ferric-siderophore reduction". 2024. http://dx.doi.org/10.1101/2024.05.13.594011.
    10.1101/2024.05.13.594011
Thesis / Dissertation
  1. Fonseca, Bruno M. "Mind the gap: characterization of periplasmic cytochromes from Shewanella oneidensis involved in extracellular electron transfer". PhD, Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, 2013.
  2. Fonseca, Bruno M. "Preliminary studies of terminal reductases from the anaerobic respiratory chains of Desulfuromonas acetoxidans". Master, Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, 2007.
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2023/10/27 Thermodynamic characterization of multiheme cytochromes: From past to future
cicCartuja - Centro de Investigaciones Científicas Isla de la Cartuja (Seville, Spain)
2023/03/15 Thermodynamic characterization of multiheme cytochromes SCAN
ITQB-NOVA (Oeiras, Portugal)
2012/01/28 A novel strategy to obtain specific isotopic labeling of multiheme cytochromes c: applications to the extracellular respiratory metabolism of Shewanella oneidensis MR-1 Bioinorganic Chemistry Gordon Research Seminar
Gordon Research Conferences (Ventura, CA, United States)
2011/10/27 Characterization of multiheme cytochromes from Shewanella oneidensis MR-1 2nd ITQB PhD Student's Meeting
ITQB-NOVA (Oeiras, Portugal)
2010/02/12 Thermodynamic characterization of multiheme cytochromes
Pacific Northwest Nacional Laboratory (Richland, WA, United States)

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2022/04/01 - Current Characterization of flavocytochrome c from Shewanella sp. DSM 9451
Supervisor of Maria Guilhermina de Oliveira Firmino
 Post graduation - Scientific Research Training A (Specialization course)
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2015 - 2016 Emerging human pathogens from Shewanella genus: understanding the molecular mechanism behind ferric iron-siderophore reduction
Co-supervisor of Inês de Brito Trindade
 Biochemistry for Health (Master)
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Event organisation

Event name
Type of event (Role) 		Institution / Organization
2006 - 2006 IV Chemistry Forum (2006/10/24 - 2006/10/25)
Conference (Member of the Organising Committee)
 Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal

Event participation

Activity description
Type of event 		Event name
Institution / Organization
2021 - Current Science week in Primary school 31 de Janeiro
Other
2007 - Current ITQB Open Day
Other
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2018 - 2018 International Microorganism Day
Exhibition
2016 - 2017 European Researchers Night
Exhibition

Jury of academic degree

Topic
Role 		Candidate name (Type of degree)
Institution / Organization
2017 Jury member of licentiate thesis in Biochemistry Alexandre Valente (Other)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal

Committee member

Activity description
Role 		Institution / Organization
2008 - 2008 Representative of the PhD students in the assembly of Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa
Other
 Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Mentoring / Tutoring

Topic Student name
2024 - 2024 Supervision of the master student Catarina Fonseca in the research project "Mapping ligand interactions in siderophore interacting proteins belonging to the Shewanella sp." under the scope of the lab rotation of the Master course 'Biochemistry for Health' Catarina Fonseca
2024 - 2024 Supervision of the master student André Bagão in the research project "Mapping ligand interactions in siderophore interacting proteins belonging to the Shewanella sp." under the scope of the lab rotation of the Master course 'Biochemistry for Health' André Bagão
2024 - 2024 Supervision of the undergraduate Biochemistry research internship of the student Beatriz Lopes from FCT-UNL Beatriz Lopes
2021 - 2021 Co-supervision of the master student Raquel Bernardino in the research project "Iron necessity meets opportunity" under the scope of the lab rotation of the Master course 'Biochemistry for Health' developed in ITQB-NOVA Raquel Bernardino
2021 - 2021 Co-supervision of the master student Tiago Assis in the research project "Iron necessity meets opportunity" under the scope of the lab rotation of the Master course 'Biochemistry for Health' developed in ITQB-NOVA Tiago Assis
2018 - 2018 Co-supervision of the master student Abeda Ahmad in the research project "Promiscuous electron transfer pathways within the Shewanella genus" under the scope of the lab rotation of the Master course 'Biochemistry for Health' developed in ITQB-NOVA Abeda Ahmad
2018 - 2018 Co-supervision of the master student Filipa Fonseca in the research project "Promiscuous electron transfer pathways within the Shewanella genus" under the scope of the lab rotation of the Master course 'Biochemistry for Health' developed in ITQB-NOVA Filipa Fonseca
2018 - 2018 Co-supervision of the master student Iara Silva in the research project "Promiscuous electron transfer pathways within the Shewanella genus" under the scope of the lab rotation of the Master course 'Biochemistry for Health' developed in ITQB-NOVA Iara Silva
2017 - 2017 Co-supervision of the work of Ana Margarida Pinto in the scope of Summer Science @ ITQB (2017) developed in ITQB-NOVA Ana Margarida Pinto
2015 - 2015 Co-supervision of Luis Silva's licentiate thesis work in Biochemistry developed in ITQB-NOVA Luis Silva

Other jury / evaluation

Activity description Institution / Organization
2018 - Current Scientific reviewer for the MDPI - Publisher of Open Access Journals
2023/10/18 - 2023/10/20 Evaluation committee of the 13th ITQB NOVA PhD Students' Meeting Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
Distinctions

Award

2019 Best poster presentation award at the ISMET 7 conference
2011 Best poster presentation award at the 3rd International Microbial Fuel Cell conference

Other distinction

2023 Staff Erasmus+ grant
2010 Graduate Student Fellowship from Stanford University
2010 Student Fellowship from the Calouste Gulbenkian Fundation
2010 Alternate Sponsored Fellowship from the Pacific Northwest National Laboratory, USA