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Identification

Personal identification

Full name
Kevin Costa Leandro

Citation names

  • Leandro, Kevin

Author identifiers

Ciência ID
A912-D4BF-7E2C
ORCID iD
0000-0003-3404-5138
Education
Degree Classification
2018/10 - 2019/08
Concluded
ERASMUS + Programme - University of Ghent (Mestrado integrado)
Universiteit Gent, Belgium
2013/09 - 2018/09
Concluded
Integrated Master's Degree in Pharmaceutical Sciences (Mestrado integrado)
Universidade de Coimbra Faculdade de Farmácia, Portugal

Università di Bologna - Università di Bologna, Italy

Universiteit Gent, Belgium
"Drug-resistant Epilepsy: An overview on the relevance of ABC Transporters Superfamily" (THESIS/DISSERTATION)
16
2016/09 - 2017/07
Concluded
ERASMUS + Programme - University of Bologna (Mestrado integrado)
Università di Bologna - Università di Bologna, Italy
2012
Concluded
First Certificate of English (FCE) (Outros)
Cambridge English Language Assessment, Portugal
B2
Affiliation

Others

Category
Host institution
Employer
2020/11/01 - Current PhD Student at CNC - Vectors, Gene and Cell Therapy Group Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2019/09/06 - 2020/10/28 Research Fellow at CNC - Vectors, Gene and Cell Therapy Group Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2018/10 - 2019/08 Erasmus Student at University of Ghent Universiteit Gent, Belgium
2018/11/05 - 2019/07 Trainee at VIB - Center for Medical Biotechnology (Proteomics Expertise Center) VIB UGent Centrum voor Medische Biotechnologie, Belgium
VIB UGent Centrum voor Medische Biotechnologie, Belgium
2018/10 - 2018/10 Trainee at CNC - Vectors, Gene and Cell Therapy Group Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2018/04/16 - 2018/08/17 Trainee in Community Pharmacy Farmácia Universal, Portugal
Farmácia Universal, Portugal
2018/01/11 - 2018/03/30 Trainee at INFARMED - Health Technology Assessment Department Infarmed Autoridade Nacional do Medicamento e Produtos de Saúde IP, Portugal
Infarmed Autoridade Nacional do Medicamento e Produtos de Saúde IP, Portugal
2017/08 - 2017/08 Trainee at Guardian Pharmatama - Quality Assurance Department Guardian Pharmatama, PT, Indonesia
2016/09 - 2017/07 Erasmus Student at University of Bologna Università degli Studi di Bologna Dipartimento di Farmacia e Biotecnologie, Italy
2016/08 - 2016/08 Trainee in Community Pharmacy Farmácia Machado, Portugal
Farmácia Machado, Portugal
2016/01 - 2016/07 Trainee at CHUC - Clinic Hematology Department Centro Hospitalar e Universitário de Coimbra EPE, Portugal
Centro Hospitalar e Universitário de Coimbra EPE, Portugal
Projects

Grant

Designation Funders
2020 - 2021 Extracellular vesicles as a platform to deliver CRISPR-Cas9 systems to treat Machado-Joseph Disease
PhD Student Fellow
European Proteomics Infrastructure Consortium providing access (EPIC-XS), Netherlands
European Proteomics Infrastructure Consortium providing access (EPIC-XS)
Ongoing

Other

Designation Funders
2020/03 - 2020/06 COVID-19 Task Force - University of Coimbra
Other
Outputs

Publications

Conference poster
  1. Leandro, Kevin; Rufino-Ramos, David; Lopes, Sara; Pasqua, Carlo; Santos, Paulo; Silva, Ana Carolina; Lobo, Diana; et al. "Extracellular vesicles-mediated delivery of CRISPR/Cas9 systems to treat Machado-Joseph Disease". Paper presented in 2023 CAG Triplet Repeat Disorder -Gordon Research Conference, 2023.
  2. Leandro, Kevin; Rufino-Ramos, David; Lopes, Sara; Pasqua, Carlo; Santos, Paulo; Silva, Ana Carolina; Lobo, Diana; et al. "Extracellular vesicles-mediated delivery of CRISPR/Cas9 systems to treat Machado-Joseph Disease". Paper presented in 2023 CAG Triplet Repeat Disorder -Gordon Research Seminar, 2023.
  3. Leandro, Kevin; Rufino-Ramos, David; D'Amelio, Tania ; Lopes, Sara; M. Martins, Inês; Lobo, Diana; Faro, Rosário; Perdigão, Pedro; Pereira de Almeida, Luis. "Extracellular vesicles-mediated delivery of CRISPR/Cas9 systems to treat Machado-Joseph Disease". Paper presented in 30th Annual Congress of the European Society of Gene & Cell Therapy, 2022.
  4. Leandro, Kevin; Rufino-Ramos, David; Lopes, Sara; M. Martins, Inês; Lobo, Diana; Paoletta, Manuela; Faro, Rosário; Perdigão, Pedro; Pereira de Almeida, Luis. "Extracellular vesicles transient delivery of CRISPR/Cas9 systems to knock-out ataxin-3 expression". Paper presented in XVII Meeting of the Portuguese Society for Neuroscience, 2021.
  5. Leandro, Kevin; Rufino-Ramos, David; Henriques, Carina; Pereira de Almeida, Luis. "Mini-Poster: Density gradient centrifugation: an improved protocol for extracellular vesicles isolation". Paper presented in CIBB Meeting 2019, 2019.
  6. Rufino-Ramos, David; Carmona, Vitor; Martins, Inês M.; Leandro, Kevin; Albuquerque, Patrícia; Faro, Rosário; Perfeito, Rita; et al. "An effective packaging cell line to highly produce Extracellular Vesicles for therapy of Machado-Joseph Disease". Paper presented in Microbiotec - Congress of Microbiology and Biotechnology 2019, 2019.
  7. Leandro, Kevin; Rufino-Ramos, David; Henriques, Carina; Pereira de Almeida, Luis. "Density Gradient Centrifugation: an improved protocol for Extracellular Vesicles isolation". Paper presented in 1st Annual Meeting in Pharmaceutical Sciences, 2019.
  8. Rufino-Ramos, David; Carmona, Vitor; Martins, Inês M.; Leandro, Kevin; Albuquerque, Patrícia; Barreira, Mário; Faro, Rosário; et al. "Extracellular Vesicles: a novel promising tool for therapy of Machado-Joseph Disease". Paper presented in 1st Annual Meeting in Pharmaceutical Sciences, 2019.
  9. Leandro, Kevin; Rufino-Ramos, David; Henriques, Carina; Pereira de Almeida, Luis. "Isolation of functional extracellular vesicles by sucrose gradient centrifugation". Paper presented in BEBday 2019, 2019.
Journal article
  1. Lopes-Nunes, Jéssica; Simões, Pedro; Moreira, David; Leandro, Kevin; Nobre, Rui Jorge; Pereira de Almeida, Luís; Campello, Maria Paula Cabral; et al. "RNA-based liposomes for oral cancer: From biophysical characterization to biological evaluation". International Journal of Biological Macromolecules 259 (2024): 129157. http://dx.doi.org/10.1016/j.ijbiomac.2023.129157.
    10.1016/j.ijbiomac.2023.129157
  2. Welsh, Joshua A.; Goberdhan, Deborah C. I.; O'Driscoll, Lorraine; Buzas, Edit I.; Blenkiron, Cherie; Bussolati, Benedetta; Cai, Houjian; et al. "Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches". Journal of Extracellular Vesicles 13 2 (2024): http://dx.doi.org/10.1002/jev2.12404.
    Published • 10.1002/jev2.12404
  3. Soares Martins, Tânia; Pelech, Steven; Ferreira, Maria; Pinho, Beatriz; Leandro, Kevin; de Almeida, Luís Pereira; Breitling, Benedict; et al. "Phosphoproteome Microarray Analysis of Extracellular Particles as a Tool to Explore Novel Biomarker Candidates for Alzheimer's Disease". International Journal of Molecular Sciences 25 3 (2024): 1584. http://dx.doi.org/10.3390/ijms25031584.
    10.3390/ijms25031584
  4. Rufino-Ramos, David; Albuquerque, Patrícia R.; Leandro, Kevin; Carmona, Vitor; Martins, Inês M.; Fernandes, Rita; Henriques, Carina; et al. "Extracellular vesicle-based delivery of silencing sequences for the treatment of Machado-Joseph disease/spinocerebellar ataxia type 3". Molecular Therapy 31 5 (2023): 1275-1292. http://dx.doi.org/10.1016/j.ymthe.2023.04.001.
    10.1016/j.ymthe.2023.04.001
  5. Rufino-Ramos, David; Leandro, Kevin; Perdigão, Pedro R.L.; O'Brien, Killian; Pinto, Maria Manuel; Santana, Magda M.; van Solinge, Thomas S.; et al. "Extracellular communication between brain cells through functional transfer of Cre mRNA mediated by extracellular vesicles". Molecular Therapy (2023): http://dx.doi.org/10.1016/j.ymthe.2023.05.012.
    10.1016/j.ymthe.2023.05.012
  6. Thery, Fabien; Martina, Lia; Asselman, Caroline; Zhang, Yifeng; Vessely, Madeleine; Repo, Heidi; Sedeyn, Koen; et al. "Ring finger protein 213 assembles into a sensor for ISGylated proteins with antimicrobial activity". Nature Communications 12 1 (2021): http://dx.doi.org/10.1038/s41467-021-26061-w.
    10.1038/s41467-021-26061-w
  7. Zhang, Yifeng; Thery, Fabien; Wu, Nicholas C.; Luhmann, Emma K.; Dussurget, Olivier; Foecke, Mariko; Leandro, Kevin; et al. "The in vivo ISGylome links ISG15 to metabolic pathways and autophagy upon Listeria monocytogenes infection". Nature Communications 10 1 (2019): http://dx.doi.org/10.1038/s41467-019-13393-x.
    Published • 10.1038/s41467-019-13393-x
  8. Leandro, Kevin; Bicker, Joana; Alves, Gilberto; Falcão, Amílcar; Fortuna, Ana. "ABC transporters in drug-resistant epilepsy: mechanisms of upregulation and therapeutic approaches". Pharmacological Research 144 (2019): 357-376. http://dx.doi.org/10.1016/j.phrs.2019.04.031.
    10.1016/j.phrs.2019.04.031
Newspaper article
  1. Vinhas, Ana Catarina; Vasconcelos-Ferreira, Ana; Henriques, Carina; Silva, Ana Carolina; Rufino-Ramos, David; Lobo, Diana; Leandro, Kevin; et. al.. "Uma viagem rara", PÚBLICO, 2020, Ciência, https://www.publico.pt/2020/02/28/ciencia/fotogaleria/viagem-rara-400481.
Thesis / Dissertation
  1. Leandro, Kevin. "Drug-resistant Epilepsy - An overview on the relevance of ABC Transporters". Master, Universidade de Coimbra Faculdade de Farmácia, 2018. https://ap1-dev.uc.pt/handle/10316/84529.

Artistic / Performance

Theatre play
  1. Viegas, Teresa Ana; Marques, Andreia; Santos, Bárbara; Henriques, Carina S; Rufino-Ramos, David; Lobo, Diana; Leandro, Kevin; et. al.. 2019. Holy CIBB.
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2024/02/08 Extracellular vesicles delivery of CRISPR-Cas9 RNPs for therapeutic gene editing in Spinocerebellar Ataxia Type 3 (presenting author) 54th Meeting of the Portuguese Society of Pharmacology
NOVA Medical School - Faculdade de Ciências Médicas (Lisbon, Portugal)
2023/11/24 Extracellular Vesicles-Mediated Delivery of CRISPR-Cas9 Systems to Treat Machado-Joseph Disease/Spinocerebellar Ataxia type 3 (presenting author) Pharma PhD Day
Faculty of Pharmacy of the University of Coimbra (Coimbra, Portugal)
2023/06/15 Extracellular Vesicles-Mediated Delivery of CRISPR/Cas9 Systems to Treat Machado-Joseph Disease (presenting author) 2023 CAG Triplet Repeat Disorder -Gordon Research Conference
Gordon Research Conference (West Dover, Vermont, United States)
2022/05/12 Optimal recovery of brain-derived extracellular vesicles (presenting author) 28th Porto Cancer Meeting
i3S (Porto, Portugal)
2022/02/10 Extracellular vesicles-mediated delivery of CRISPR/Cas9 systems for therapeutic genome editing (presenting author) 52nd Meeting of the Portuguese Society of Pharmacology
ICBAS-UP (Porto, Portugal)
2021/11/14 Extracellular communication mediated by EVs in mouse brain (co-author) 1st Meeting of the Portuguese Network on Extracellular Vesicles
i3S (Porto, Portugal)
2021/09/14 Extracellular vesicles-mediated delivery of CRISPR/Cas9 tools to treat Machado-Joseph Disease (presenting author) 1st Meeting of the Portuguese Network on Extracellular Vesicles
i3S (Porto, Portugal)
2021/09/14 Extracellular vesicles: a promising delivery system for silencing sequences to treat Machado-Joseph Disease (co-author) 1st Meeting of the Portuguese Network on Extracellular Vesicles
i3S (Porto, Portugal)
2020/02/06 Extracellular Vesicles for treatment of Machado-Joseph Disease (co-author) SPF Meeting 2020 - Reunião Anual da Sociedade Portuguesa de Farmacologia
Faculdade de Farmácia da Universidade de Coimbra (Coimbra, Portugal)
2019/12/06 An effective packaging cell line to highly produce Extracellular Vesicles for therapy of Machado-Joseph Disease (co-author) Microbiotec - Congress of Microbiology and Biotechnology 2019
Faculty of Sciences and Technology of the University of Coimbra (Polo II) (Coimbra, Portugal)
2019/11/08 An extracellular vesicle-based system to deliver silencing sequences for therapy of Machado-Joseph disease/ Spinocerebellar Ataxia type-3 (co-author) 5th International GEIVEX 2019 Symposium
Granada, Spain (Granada, Spain)

Association member

Society Organization name Role
2022 - Current International Society for Extracellular Vesicles (ISEV) Member
2021/09 - Current Portuguese Society for Neuroscience (SPN) Member
2020/02/27 - Current European Society of Gene and Cell Therapy (ESGCT) Member
Distinctions

Award

2022 1st Prize at "UC 3 Minute-Thesis Competition (3MT)"
Universidade de Coimbra, Portugal
2021 1st Prize at "EIT Health i-Days" - European Health Innovation Competition
2020 1º Prize at "Building Tomorrow Together" - Acceleration and Open Innovation Competition promoted by Roche Portugal
Roche Portugal, Portugal
2020 1st Prize at "Beyond the Lab" - Entrepreneurship Bootcamp for Scientists
Chaperone - Platform for personalised career support for scientists, Portugal