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I have a Bachelor’s Degree in Biochemistry from the Faculty of Science and Technology of the University of Coimbra (2018-2021). During my Bachelor’s, in my Undergraduate Project, I studied the radiobiological effects of Radium-223 in 3D spheroids of prostate carcinoma, which allied to the Specialization Course in “Cell Culture and Tissue Training” that I did, allowed me to learn the basilar skills of cell culture. Besides this, I have a Master’s Degree in Clinical Laboratory Genetics from the Faculty of Medicine of the University of Coimbra (2021-2023). In my thesis, entitled “Genetic Analysis of Patients with Frontotemporal Dementia and Generation of induced Pluripotent Stem Cells and Brain Organoids”, I had two main goals. Firstly, I employed Sanger Sequencing and NGS to diagnose Frontotemporal Dementia patients with mutations in GRN and C9ORF72 genes, under the supervision of Maria do Rosário Almeida in the Neurogenetics Laboratory of CNC. Secondly, I established human induced pluripotent stem cells (iPSCs) from patient-derived fibroblasts and derived human 3D brain organoids from these lines, in the Neuronal Circuits and Behavior Laboratory of CNC under the supervision of Ana Luísa Cardoso and João Peça. This gave me valuable insights and knowledge in advanced cell culture techniques, cell and tissue immunostaining, confocal microscopy, real-time PCR and cryosectioning. My work allowed me to present a poster entitled “Generation and Characterization of induced pluripotent stem cells derived from human dermal fibroblast lines” at FENS Regional Meeting. I also attended the VII Symposium Portuguese Glial Network, where I was co-author of a poster. Until June, I was awarded a 6-month fellowship, in the Advanced Therapies Group, under the supervision of Susana Rosa and Lino Ferreira. Here, I had the opportunity not only to delve deeper into immunofluorescence techniques and data analysis but also to expand my skill set to include Flow Cytometry and the differentiation of human iPSCs (derived from patients with POMPE disease) into skeletal muscle cells, to generate disease models and for drug screening tests to uncover novel therapeutic targets for rare muscular disorders.
Identification

Personal identification

Full name
Mafalda Teixeira Vaz das Neves

Citation names

  • Neves, Mafalda

Author identifiers

Ciência ID
A91C-BE57-F445
ORCID iD
0009-0001-3011-1997

Email addresses

  • mafaldatneves@gmail.com (Professional)

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese (Mother tongue)
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Education
Degree Classification
2024/01/02 - 2024/07/01
Ongoing
Clinical Investigator Certificate Level 1 (Outros)
Universidade NOVA de Lisboa NOVA Medical School, Portugal

Universidade de Aveiro, Portugal
2021/11/04 - 2023/10/25
Concluded
Clinical Laboratory Genetics (Mestrado)
Universidade de Coimbra Faculdade de Medicina, Portugal
"Genetic Analysis of Patients with Frontotemporal Dementia and Generation of induced Pluripotent Stem Cells and Brain Organoids" (THESIS/DISSERTATION)
17
2021/06/30 - 2021/08/28
Concluded
The Psychology of Criminal Justice (Outros)
The University of Queensland, Australia

edX LLC, United States
2021/07/02 - 2021/07/23
Concluded
VIII Cell Culture and Tissue Training Course (Outros)
Universidade de Coimbra Faculdade de Medicina, Portugal
2018/09/13 - 2021/07/13
Concluded
Biochemistry (Licenciatura)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
15
Affiliation

Science

Category
Host institution
Employer
2024/01/02 - 2024/07/01 Researcher (Research) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal

Others

Category
Host institution
Employer
2022/06/06 - 2022/06/11 Laboratory Rotation in Cytogenetics and Molecular Cytogenetics Laboratório de Citogenética do Centro Hospitalar de Trás-os-Montes e Alto Douro, Portugal
Laboratório de Citogenética do Centro Hospitalar de Trás-os-Montes e Alto Douro, Portugal
2022/01/10 - 2022/01/14 Laboratory Rotation in Oncology Universidade de Coimbra - Laboratório de Oncobiologia e Hematologia - Instituto de Investigação Clínica e Biomédica, Portugal
Universidade de Coimbra Faculdade de Medicina, Portugal
2021/02/15 - 2021/06/04 Laboratory Internship inserted in the Curricular Unit "Project" in the project "Study of the radiobiological effects of Radium-223 in 3D culture models (spheroids) of prostate carcinoma" Universidade de Coimbra Instituto de Investigação Clínica e Biomédica de Coimbra, Portugal
Universidade de Coimbra Instituto de Investigação Clínica e Biomédica de Coimbra, Portugal
2020/09/15 - 2020/12/13 Laboratory Internship in Microbiology Universidade de Coimbra Departamento de Ciências da Vida, Portugal
Universidade de Coimbra Departamento de Ciências da Vida, Portugal
2020/08/10 - 2020/09/01 Laboratory Internship in Clinical Analysis Egianálises Lac, LDA, Portugal
Egianálises Lac, LDA, Portugal
Projects

Contract

Designation Funders
2024/01/02 - 2024/07/01 Drug REpurposing with Artificial intelligence for Muscular disorderS
101080229
Research Fellow
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
European Commission
Ongoing
Outputs

Publications

Conference poster
  1. Neves, Mafalda; Martins, Solange; Cardoso, Ana. Corresponding author: Cardoso, Ana. "Generation and characterization of induced pluripotent stem cells derived from human dermal fibroblast lines". Paper presented in FENS Regional Meeting, 2023.
  2. Martins, Solange; Neves, Mafalda; Martins, Mariana; Cardoso, Ana Maria; Almeida, Maria Rosário; Santana, Isabel; Peça, João; Cardoso, Ana L.. Corresponding author: Cardoso, Ana L.. "Immunocompetent brain organoids and induced-microglia-like cells to study the pathological mechanisms of Frontotemporal Dementia". Paper presented in FENS Regional Meeting, Algarve, Portugal, 2023.
Activities

Event participation

Activity description
Type of event
Event name
Institution / Organization
2023/05/03 - 2023/05/05 FENS Regional Meeting 2023
Meeting
FENS Regional Meeting 2023
2023/05/02 - 2023/05/02 VII Symposium Portuguese Glial Network
Symposium
VII Symposium Portuguese Glial Network
2022/11/29 - 2022/11/29 Testes Genéticos em Contexto: pessoas, famílias, sociedade - Evento comemorativo dos 25 anos de testes genéticos no IBMC
Symposium
Testes Genéticos em Contexto: pessoas, famílias, sociedade
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2020/05/04 - 2020/05/29 Bioeconomy: For a sustainable future
Conference
Bioeconomy: For a sustainable future
Universidade de Coimbra Departamento de Ciências da Vida, Portugal
2019/04/12 - 2019/04/15 XII ENEBIOQ - Encontro Nacional de Estudantes de Bioquímica
Meeting
XII ENEBIOQ - Encontro Nacional de Estudantes de Bioquímica
Universidade de Aveiro, Portugal
2019/04/13 - 2019/04/13 Goodbye flat and static biology: liquified and multilayered capsules for tissue regeneration in XXI ENEBIOQ - Encontro Nacional de Estudantes de Bioquímica
Workshop
Goodbye flat and static biology: liquified and multilayered capsules for tissue regeneration
Universidade de Aveiro, Portugal
2019/04/13 - 2019/04/13 Animal Testing in XII ENEBIOQ - Encontro Nacional de Estudantes de Bioquímica
Workshop
Animal Testing
Universidade de Aveiro, Portugal

Association member

Society Organization name Role
2020/09/11 - 2021/06/04 Núcleo de Estudantes de Bioquímica da Associação Académica de Coimbra Coordenadora do Pelouro de Pedagogia
2019/10/10 - 2020/06/10 Núcleo de Estudantes de Bioquímica da Associação Académica de Coimbra Colaboradora do Pelouro de Relações Externas