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My first experience in research dating back to my trainee of degree in biochemist (FCUP, 2001-2005). My work has been focused on the "Study the function of protein phosphatase Sit4 in response to oxidative stress and chronological aging in Saccharomyces cerevisiae". In the end of 2005, I started a research fellowship of 36 months (project FCT/POCI/SAU/MMO/55374/2004), and over this period I developed my master degree in Molecular Medicine and Oncology (FMUP, 2006-2008) with the thesis title "Neuronal Ceroid Lipofuscinosis: Studies of cellular localization of CLN6 protein". In 2008, FCT attributes me a doctoral fellowship (FCT/SFRH/BD/41759/2007) and I started my doctoral degree in Biomedical Sciences (ICBAS-UP, 2008-2013) with the thesis title "Molecular pathophysiology underlying the neonatal form of Farber disease". In the following two years, I was involved in a FCT project (PTDC/BIA-PLA/2291/2012) titled "Regulation of Glutamine synthetase by Nitric Oxide in Medicago truncatula" as post- doctoral fellow (IBMC). In 2017 to present, I joined the Institute of Biomedicine (iBiMED) of University of Aveiro, as Light Microscopy Facility Manager. Expertise topics: Biomedical Sciences, Biochemistry, Molecular Biology, Genetics, Widefield and Laser-Scanning Confocal Microscopy, BioImaging.
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Identification

Personal identification

Full name
Mariana Isabel Quaresma da Rocha Alves

Citation names

  • Alves, MQ

Author identifiers

Ciência ID
BC11-BC23-0973
ORCID iD
0000-0003-1935-192X

Telephones

Telephone
  • 234247230 (Professional)

Websites

Knowledge fields

  • Medical and Health Sciences - Health Sciences

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
English Intermediate (B1) Intermediate (B1) Intermediate (B1) Intermediate (B1)
Education
Degree Classification
2018/09/26 - 2018/09/28
Concluded
 Introduction to digital image analysis with ImageJ / Fiji 2nd Edition Course (Outros)
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2018/07/03 - 2018/07/04
Concluded
 IMARIS (Image Analysis Software) Training Course (Outros)
Instituto Gulbenkian de Ciência, Portugal
2017/09/18 - 2017/09/22
Concluded
 III Quantitative Fluorescence Microscopy Course (Outros)
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2017/09/11 - 2017/09/14
Concluded
 Network of European BioImage Analysts (NEUBIAS) 2nd Training School for Early Career Researchers (TS4), “Basics of Image Analysis, Processing and Workflows using ImageJ & CellProfiler (Postgraduate Certificate)
Göteborgs Universitet, Sweden
2008/09/01 - 2013/12/20
Concluded
 Doutoramento em Ciências Biomédicas (Doutoramento)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
"Molecular pathophysiology underlying the neonatal form of Farber disease." (THESIS/DISSERTATION)
 Aprovado
2011/09/29 - 2011/10/01
Concluded
 International Symposium on Lysosomes (Postgraduate Certificate)
Universitätsklinikum Hamburg-Eppendorf, Germany
2011 - 2011
Concluded
 Direitos Tecnológicos e Pedido Provisório de Patente (Curso médio)
Instituto Nacional de Saúde Doutor Ricardo Jorge - PORTO, Portugal
2011 - 2011
Concluded
 International Symposium on Lysosomes (Curso médio)
Universität Hamburg, Germany
2011 - 2011
Concluded
 Ciência em Animais de Laboratório (Categoria B) (Curso médio)
Universidade do Porto, Portugal
2011 - 2011
Concluded
 Workshop de Máquinas Digitais (Outros)
Universidade do Porto, Portugal
2009 - 2009
Concluded
 Mass Spectrometry: An interdisciplinary approach” (Outros)
Universidade do Porto, Portugal
2009 - 2009
Concluded
 Redação científica para jovens investigadores (Curso médio)
Universidade do Porto, Portugal
2009 - 2009
Concluded
 A Metabonómica na Investigação e Prática Clínicas (Curso médio)
Universidade de Aveiro, Portugal
2006 - 2008
Concluded
 Mestrado em Medicina e Oncologia Molecular (Mestrado)
Universidade do Porto Faculdade de Medicina, Portugal
"Ceroido Lipofuscinose Neuronal. Estudos de localização celular da proteína CLN6." (THESIS/DISSERTATION)
 Muito Bom
2007 - 2007
Concluded
 Curso de Bioinformática (Curso médio)
Universidade do Minho, Portugal
2007 - 2007
Concluded
 Formação Pedagógica Inicial de Formadores - CAP (Curso médio)
Instituto do Emprego e Formação Profissional, Portugal
2006 - 2006
Concluded
 Combate a Incêndios com Extintores (Curso médio)
Centro de Genética Médica Doutor Jacinto Magalhães, Portugal
2001 - 2005
Concluded
 Licenciatura em Bioquímica (Licenciatura)
Universidade do Porto Faculdade de Ciências, Portugal
"Estudo da função da proteína fosfatase Sit4 na resposta ao stress oxidativo e no envelhecimento cronológico em Saccharomyces cerevisiae." (THESIS/DISSERTATION)
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Affiliation

Science

Category
Host institution 		Employer
2017/05/08 - 2021/10/05 Science and Technology Management Universidade de Aveiro Instituto de Biomedicina, Portugal
2005 - 2005 Research Trainee (Research) Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal

Others

Category
Host institution 		Employer
2021/01/15 - Current Técnico Superior Universidade de Aveiro Instituto de Biomedicina, Portugal
Universidade de Aveiro Instituto de Biomedicina, Portugal
2014/01/01 - 2015/09/30 Post-doctoral fellow Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2013/09/01 - 2014/07/01 Professor Escola Superior de Enfermagem da Cruz Vermelha Portuguesa de Oliveira de Azeméis, Portugal
2008/09/01 - 2013/12/20 PhD Fellow Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
Instituto Nacional de Saúde Doutor Ricardo Jorge - PORTO, Portugal
2005/11 - 2008/04 Research Fellow Centro de Genética Médica Doutor Jacinto Magalhães, Portugal
2006 - 2008 Master Fellow Universidade do Porto Faculdade de Medicina, Portugal
Instituto Nacional de Saúde Doutor Ricardo Jorge - PORTO, Portugal
Projects

Contract

Designation Funders
2018/06/22 - 2021/06/21 GoBack - New therapeutic targets for spinal cord injuries./"Novos alvos terapêuticos para lesões medulares."
CENTRO-01-0145-FEDER-032261
team member, expertise in confocal microscopy
Universidade de Aveiro, Portugal
 N/A
Ongoing
2017/05/08 - 2021/04/30 pAGE - Protein aggregation across the lifespan./"Agregação proteica ao longo da vida."
Centro-01-0145-FEDER-000003
team member of the iBiMED Research Unit
Universidade de Aveiro, Portugal
Ongoing
2017/06/01 - 2020/05/30 PPBI-Portuguese Bioimaging Platform./"Plataforma Portuguesa de Bioimagem."
POCI-01-0145-FEDER-022122
Light microscopy facility manager
Universidade de Aveiro, Portugal
Concluded
2014/01 - 2015/07 Regulation of Glutamine synthetase by Nitric Oxide in Medicago truncatula./"Regulação da Glutamina sintetase pelo Oxido Nítrico em Medicago truncatula."
PTDC/BIA-PLA/2291/2012
 N/A
2005/11 - 2008/04 Neuronal Ceroid Lipofuscinosis: Molecular, biochemist and functional studies./"Ceroido Lipofuscinose Neuronal: estudo molecular, bioquímico e funcional."
FCT/POCI/SAU-MMO/55374/2004
 N/A
Outputs

Publications

Conference poster
  1. "Protein aggregation detection: new fluorophores". Paper presented in Workshop: Strategies & tools for modulating pathologic protein self-assembly, 2019.
  2. "Regulation of the plastid located glutamine synthetase by s-nitrosylation in Medicago truncatula.". Paper presented in 12th International Conference on Reactive Oxygen and Nitrogen Species in Plants: from model systems to field,, 2015.
  3. Silva, L.; Melo, P.; Alves, M.; Carvalho, H.. "The plastid-located Glutamine Synthetase is regulated by S-nitrosylation in Medicago truncatula". Paper presented in 5th Plant NO Club Meeting, 2014.
  4. Alves, M.; Ribeiro, I.; Ferreira, N.; Levade, T.; Ribeiro, MG.. "Molecular mechanism of acid ceramidase deficiency caused by a specific a-subunit mutation.". Paper presented in 18th ESGLD Workshop, 2011.
  5. Ferreira, N.; Alves, M.; Ribeiro, I.; Bittman, R.; Pagano, R.; Levade, T.; Ribeiro, MG.. "Lipid Trafficking Alterations in Acid Ceramidase-deficient Human Fibroblasts". Paper presented in 35th FEBS Congress, 2010.
  6. Alves, M.; Ribeiro, I.; Ribeiro, MG.. "Comparative study of Acid Ceramidase immunoreactivity in blood cells, fibroblasts and amniocytes". Paper presented in 17th ESGLD Workshop,, 2009.
  7. Ribeiro, MG.; Ribeiro, I.; Alves, M.; Ferreira, N.; Amaral, O.; Lopes, L.. "Research and diagnosis of Farber disease in Portugal". Paper presented in VI International Symposium of the Portuguese Society for Metabolic Diseases, 2009.
  8. Alves, M.; Bessa, C.; Teixeira, CA.; Boustany, R-M; Ribeiro, MG.. "Novel clues on the intracellular localization of CLN6, the protein defective in the neurodegenerative LSD-NCL type 6". Paper presented in Annual Symposium of the Society for the Study of Inborn Errors of Metabolism, 2008.
  9. Ribeiro, I.; Alves, M.; Amaral, O.; Ribeiro, MG.. "Biochemical screening of Farber Disease using a combined approach.". Paper presented in Annual Symposium of the Society for the Study of Inborn Errors of Metabolism, 2008.
  10. Teixeira, CA.; Bessa, C.; Alves, M.; Ribeiro, MG.. "Lipid trafficking imbalance in CLN6 disease.". Paper presented in FEBS Workshop, 2008.
  11. Bessa, C.; Alves, M.; Teixeira, CA.; Lacerda, L.; Ribeiro, MG.. "The CLN5 protein defective in the Finnish vLINCL is a junctional plasma membrane protein.". Paper presented in 11th International Congress of Neuronal Ceroid Lipofuscinosis (Batten disease), 2007.
  12. Bessa, C.; Teixeira, CA.; Dias, A.; Alves, M.; Rocha, S.; Lacerda, L.; Guimarães, A.; Ribeiro, MG.. "CLN2/TPP1 deficiency: The novel mutation IVS7-10 A>G is associated with a mild disease phenotype". Paper presented in IV Internacional Symposium - Sociedade Portuguesa Doenças Metabólicas, 2006.
Journal article
  1. Fátima Liliana Monteiro; André Góis; Inês Direito; Tânia Melo; Bruna Neves; Mariana I. Alves; Inês Batista; Maria do Rosário Domingues; Luisa Helguero. "Inhibiting SETD7 methyl-transferase activity impairs differentiation, lipid metabolism and lactogenesis in mammary epithelial cells". FEBS Letters (2023): http://dx.doi.org/10.1002/1873-3468.14737.
    10.1002/1873-3468.14737
  2. Joana R. M. Ferreira; Mariana Alves; Bárbara M. de Sousa; Sandra I. Vieira; Artur M. S. Silva; samuel guieu; Ângela Cunha; Nunes da Silva, R.. "Curcumin-based molecular probes for fluorescence imaging of fungi". Organic & Biomolecular Chemistry (2023): http://dx.doi.org/10.1039/d2ob01872a.
    10.1039/d2ob01872a
  3. "Liprobe, a vital dye for lipid aggregates detection in imaging and high-content screens". ACS Sensors (2022):
    Submitted
  4. Silva, Liliana S.; Alves, Mariana Q.; Seabra, Ana R.; Carvalho, Helena G.. "Characterization of plant glutamine synthetase S-nitrosation". Nitric Oxide 88 (2019): 73-86. http://dx.doi.org/10.1016/j.niox.2019.04.006.
    10.1016/j.niox.2019.04.006
  5. Raquel Nunes da Silva; Catarina C. Costa; Maria J. G. Santos; Mariana Q. Alves; Susana S. Braga; Sandra I. Vieira; João Rocha; Artur M. S. Silva; Samuel Guieu. "Fluorescent Light-up Probe for the Detection of Protein Aggregates". Chemistry – An Asian Journal 14 6 (2019): 859-863. https://doi.org/10.1002/asia.201801606.
    10.1002/asia.201801606
  6. Alves, Mariana Q.; Le Trionnaire, Emmanuelle; Ribeiro, Isaura; Carpentier, Stéphane; Harzer, Klaus; Levade, Thierry; Ribeiro, M. Gil. "Molecular basis of acid ceramidase deficiency in a neonatal form of Farber disease: Identification of the first large deletion in ASAH1 gene". Molecular Genetics and Metabolism 109 3 (2013): 276-281. http://dx.doi.org/10.1016/j.ymgme.2013.04.019.
    Published • 10.1016/j.ymgme.2013.04.019
  7. Barbosa, António Daniel; Osório, Hugo; Sims, Kellie J.; Almeida, Teresa; Alves, Mariana; Bielawski, Jacek; Amorim, Maria Amélia; et al. "Role for Sit4p-dependent mitochondrial dysfunction in mediating the shortened chronological lifespan and oxidative stress sensitivity of Isc1p-deficient cells". Molecular Microbiology 81 2 (2011): 515-527. http://dx.doi.org/10.1111/j.1365-2958.2011.07714.x.
    Published • 10.1111/j.1365-2958.2011.07714.x
  8. Bessa, C.; Teixeira, CA.; Dias, A.; Alves, M.; Rocha, S.; Lacerda, L.; Loureiro, L.; Guimarães, A.; Ribeiro, MG.. "CLN2/TPP1 deficiency: The novel mutation IVS7-10A>G causes intron retention and is associated with a mild disease phenotype". Molecular Genetics and Metabolism 93 1 (2008): 66-73. http://dx.doi.org/10.1016/j.ymgme.2007.08.124.
    Published • 10.1016/j.ymgme.2007.08.124
Magazine article
  1. Maria Gil Ribeiro; Natália Ferreira; Mariana Alves; Isaura Ribeiro. "Lipid imbalance in the progressive metabolic disorder, Farber disease", Revista da Faculdade de Ciências da Saúde, Universidade Fernando Pessoa, 2015, http://hdl.handle.net/10284/2986.
Report
  1. Alves, MQ. 2013. Molecular pathophysiology underlying the neonatal form of Farber disease.
  2. Alves, MQ. 2008. Ceroido Lipofuscinose Neuronal. Estudos de localização celular da proteína CLN6..
  3. Alves, MQ. 2005. Estudo da função da proteína fosfatase Sit4 na resposta ao stress oxidativo e no envelhecimento cronológico em Saccharomyces cerevisiae..
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2010 Lipid Trafficking Alterations in Acid Ceramidase-deficient Human Fibroblasts 35th FEBS Congress,
Federation of European Biochemical Societies (Gothenburg, Sweden)
2007 The CLN5 protein defective in the Finnish vLINCL is a junctional plasma membrane protein. 11th International Congress of Neuronal Ceroid Lipofuscinosis (Batten disease)
(Rochester, New York, United States)

Event organisation

Event name
Type of event (Role) 		Institution / Organization
2023/05/16 - 2023/05/16 MicroDay - Nacional Day of Microscopy. The MicroDay was a national initiative, on the framework of the PPBI, and where the microscopy research infrastructures from 13 nodes of PPBI open their door to under-graduated students. (2023/05/16 - 2023/05/16)
Other (Member of the Organising Committee)
 Universidade de Aveiro Instituto de Biomedicina, Portugal

Portuguese Platform of BioImaging, Portugal
2019/05/22 - 2019/05/22 MicroDay - Nacional Day of Microscopy. The MicroDay was a national initiative, on the framework of the PPBI, and where the microscopy research infrastructures from 13 nodes of PPBI open their door to under-graduated students. (2019/05/22 - 2019/05/22)
Workshop (Co-organisor)
 Universidade de Aveiro, Portugal
2014 - 2014 Seminário de Nutrição. Nutrição: Diferentes Olhares
Seminar (Other)
2011 - 2011 Semana Aberta do INSA, 2011 - Era uma vez
Workshop (Other)
 INSA, IP, Portugal
2010 - 2010 Semana Aberta do INSA, 2010 - Viagem pela Saúde
Workshop (Other)
 Instituto Nacional de Saúde Doutor Ricardo Jorge, Portugal

Event participation

Activity description
Type of event 		Event name
Institution / Organization
2011 - 2011 Molecular mechanism of acid ceramidase deficiency caused by a specific a-subunit mutation.
Congress
 18th ESGLD Workshop
European Study Group on Lysosomal Diseases, Finland
2010 - 2010 Lipid Trafficking Alterations in Acid Ceramidase-deficient Human Fibroblasts.
Congress
 35th FEBS Congress
Federation of European Biochemical Societies, Sweden
2009 - 2009 Comparative study of Acid Ceramidase immunoreactivity in blood cells, fibroblasts and amniocytes.
Congress
 17th ESGLD Workshop
European Study Group on Lysosomal Diseases, Germany
2009 - 2009 Doenças Raras: do indivíduo à sociedade. Congresso Científico dos Alunos do 5º ano da FFUP 2009.
Congress
 Doenças Raras: do indivíduo à sociedade. Congresso Científico dos Alunos do 5º ano da FFUP 2009.
Alunos do 5º ano da FFUP., Portugal
2008 - 2008 Novel clues on the intracellular localization of CLN6, the protein defective in the neurodegenerative LSD-NCL type 6.
Congress
 Annual Symposium of the Society for the Study of Inborn Errors of Metabolism
Society for the Study of Inborn Errors of Metabolism, Portugal
2008 - 2008 Biochemical screening of Farber Disease using a combined approach.
Congress
 Annual Symposium of the Society for the Study of Inborn Errors of Metabolism
Society for the Study of Inborn Errors of Metabolism, Portugal
2007 - 2007 CLN2/TPP1 deficiency: the novel mutation IVS-7A>G is associated with a mild disease phenotype
Congress
 16th ESGLD Workshop
European Study Group on Lysosomal Diseases, Italy
2006 - 2006 CLN2/TPP1 deficiency: The novel mutation IVS7-10 A>G is associated with a mild disease phenotype.
Congress
 IV International Symposium SPDM: Inborn Errors of Metabolism: A Permanent Challenge
Sociedade Portuguesa de Doenças Metabólicas, Portugal
2002 - 2002 XIII Congresso Nacional de Bioquímica
Congress
 XIII Congresso Nacional de Bioquímica
Sociedade Portuguesa de Bioquímica, Portugal

Association member

Society Organization name Role
2020 - Current PPBI Euro-Bioimaging Node Light Microscopy facility Manager

Course / Discipline taught

Academic session Degree Subject (Type) Institution / Organization
2014/03 - 2014/07 Nutrição (Outros) Escola Superior de Enfermagem da Cruz Vermelha Portuguesa de Oliveira de Azeméis, Portugal
2013/09 - 2014/02 Farmacologia (Outros) Escola Superior de Enfermagem da Cruz Vermelha Portuguesa de Oliveira de Azeméis, Portugal
2013/09 - 2014/02 Bioquímica (Outros) Escola Superior de Enfermagem da Cruz Vermelha Portuguesa de Oliveira de Azeméis, Portugal
2013/09 - 2014/02 Microbiologia (Outros) Escola Superior de Enfermagem da Cruz Vermelha Portuguesa de Oliveira de Azeméis, Portugal