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Sofia de Oliveira Dias Duarte. Completed the Mestrado integrado in Microbiologia Aplicada in 2011 by Universidade de Lisboa Faculdade de Ciências, Doutoramento in Biotecnologia e Biociências in 2018/04/16 by Universidade de Lisboa Instituto Superior Técnico, Licenciatura in Psicologia in 2005 by Universidade de Lisboa Faculdade de Psicologia and Licenciatura in Biologia in 2009 by Universidade de Lisboa Faculdade de Ciências. Is Researcher in Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento. Published 14 articles in journals. Has 2 section(s) of books. Organized 1 event(s). Participated in 8 event(s). Supervised 5 MSc dissertation(s) e co-supervised 1. Supervised 1 work(s) of course completion of LSc/BSc e co-supervised 1. Has received 4 awards and/or honors. Participates and/or participated as PhD Student Fellow in 1 project(s) and Researcher in 1 project(s). Works in the area(s) of Natural sciences with emphasis on Biological Sciences with emphasis on Molecular Biology, Natural sciences with emphasis on Biological Sciences with emphasis on Molecular Biology, Natural sciences with emphasis on Biological Sciences with emphasis on Microbiology, Medical and Health Sciences with emphasis on Medical Biotechnology with emphasis on Health-Related Biotechnology, Natural sciences with emphasis on Biological Sciences with emphasis on Biochemistry, Medical and Health Sciences with emphasis on Basic Medicine, Medical and Health Sciences with emphasis on Medical Biotechnology with emphasis on Gene-based Diagnostics and Therapeutic Interventions, Natural sciences with emphasis on Biological Sciences with emphasis on Genetics and Heredity, Medical and Health Sciences with emphasis on Medical Biotechnology with emphasis on Technologies Involving the Manipulation of Cells, Tissues, Organs or the Whole Organism, Exact Sciences with emphasis on Physical Sciences with emphasis on Optics, Engineering and Technology with emphasis on Civil Engineering with emphasis on Municipal and Structural Engineering and Engineering and Technology with emphasis on Nano-technology. In their professional activities interacted with 142 collaborator(s) co-authorship of scientific papers. In his curriculum Ciência Vitae the most frequent terms in the context of scientific, technological and artistic-cultural output are: Microbiologia; Saccharomyces bayanus; Saccharomyces cerevisiae; Etanol; Cálcio; Live delivery vectors; Escherichia coli; Lactococcus lactis; Lactobacillus; Lactococcus; PCR; cDNA; mRNA; CRISPR-Cas9; Gibson Assembly; Biobricks; Knockouts; HPLC; Recombineering; ELISA; Bacillus pasteurii; Urease; Förster Resonance Energy Transfer; Synthetic Biology; Cloning; pDNA engineering; Protein engineering; Strain engineering; Recombinant proteins; Recombinant DNA; Recombinant strains; Plasmid Copy Number; Site-directed mutagenesis; Homologous recombination; Fusion protein; Cell factory; Metabolic engineering; Random mutagenesis; Genome engineering; Restriction enzymes; Agarose gel; Quantitative real-time PCR; Plasmid DNA; Genomic DNA; Reverse transcription qPCR; Reverse transcription; Proteins; Nucleic Acid purification; Nucleic acid quantification; Metabolite quantification; Lactic Acid Bacteria; Gram-positive bacteria; Gram-negative bacteria; Yeast; Animal cells; Stem cells; Cell factories; Heat shock transformation; Electroporation; Curcumin; DNA vaccines; Cytokines; Liposomes; Liposomes/pDNA complex; Comparative genomics; Sequence analysis; Confocal microscopy; Biocimentation; Biological Engineering and Biotechnology - Biotechnology; Systems Biology; Therapeutic agents; Cancer; pTRKH3; Ribosome Binding Site; Enzymes; Microfluidics; Soil; Pore clogging; Ground improvement; Clays; Tensile strength; Porosimetry; Osmotic effects on clay; Biocement; Bacteria; Rehabilitation; Protection; Porous media; Urease-based biocementation; Nanomedicine; Diabetes; Biodegradable polymeric nanoparticles; Synthetic biology; Lactic acid bacteria; A
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Identification

Personal identification

Full name
Sofia de Oliveira Dias Duarte

Citation names

  • Duarte, Sofia
  • Duarte, Sofia O. D.
  • Duarte, Sofia Oliveira Dias
  • Duarte, S. O.
  • Duarte, S.
  • Duarte, S. O. D.
  • Duarte, Sofia O.

Author identifiers

Ciência ID
BC1B-B8AB-73BC
ORCID iD
0000-0002-7945-1474

Email addresses

  • sofia.duarte@tecnico.ulisboa.pt (Professional)

Telephones

Telephone
  • 218419130 (Professional)

Addresses

  • IBB-Institute for Bioengineering and Biosciences, BioEngineering Research Group, Instituto Superior Técnico. Av. Rovisco Pais, 1, 1049-001, Lisboa, Lisboa, Portugal (Professional)

Websites

Knowledge fields

  • Natural sciences - Biological Sciences - Molecular Biology
  • Natural sciences - Biological Sciences - Molecular Biology
  • Natural sciences - Biological Sciences - Microbiology
  • Medical and Health Sciences - Medical Biotechnology - Health-Related Biotechnology
  • Natural sciences - Biological Sciences - Biochemistry
  • Medical and Health Sciences - Medical Biotechnology - Gene-based Diagnostics and Therapeutic Interventions
  • Medical and Health Sciences - Basic Medicine
  • Natural sciences - Biological Sciences - Genetics and Heredity
  • Medical and Health Sciences - Medical Biotechnology - Technologies Involving the Manipulation of Cells, Tissues, Organs or the Whole Organism
  • Exact Sciences - Physical Sciences - Optics
  • Engineering and Technology - Civil Engineering - Municipal and Structural Engineering
  • Engineering and Technology - Nano-technology

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese (Mother tongue)
English Proficiency (C2) Proficiency (C2) Proficiency (C2) Proficiency (C2) Proficiency (C2)
French Elementary (A2) Upper intermediate (B2) Elementary (A2) Upper intermediate (B2) Elementary (A2)
Education
Degree Classification
2013/06/01 - 2018/04/16
Concluded
 Biotecnologia e Biociências (Doutoramento)
Universidade de Lisboa Instituto Superior Técnico, Portugal
"Lactic acid bacteria as cell factories: A synthetic biology approach for plasmid DNA and recombinant protein production" (THESIS/DISSERTATION)
 Approved with Distinction
2009 - 2011
Concluded
 Microbiologia Aplicada (Mestrado integrado)
Universidade de Lisboa Faculdade de Ciências, Portugal
"The role of calcium in Saccharomyces sp. response to ethanol stress" (THESIS/DISSERTATION)
 18
2006 - 2009
Concluded
 Biologia (Licenciatura)
Universidade de Lisboa Faculdade de Ciências, Portugal
"n/a" (THESIS/DISSERTATION)
 17
2000 - 2005
Concluded
 Psicologia (Licenciatura)
Universidade de Lisboa Faculdade de Psicologia, Portugal
"N/a" (THESIS/DISSERTATION)
 15
Affiliation

Science

Category
Host institution 		Employer
2023/04/10 - Current Researcher (Research) Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
2022/03/21 - 2022/12/31 Researcher (Research) Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
2019/02/01 - 2021/09/30 Contracted Researcher (Research) Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
2018/07/01 - 2019/01/31 Researcher (Research) Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
2011/12/01 - 2013/05/31 Researcher (Research) Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal

Others

Category
Host institution 		Employer
2013/06/01 - 2018/04/16 PhD scolarship Fundação para a Ciência e a Tecnologia, Portugal
Associação para o Desenvolvimento do Instituto Superior Técnico, Portugal
Projects

Grant

Designation Funders
2013/06/01 - 2017/12/31 HETEROLOGOUS PRODUCTION OF CURCUMIN BY PROBIOTIC LACTIC ACID BACTERIA TOWARDS GASTROINTESTINAL CANCER THERAPY
SFRH/BD/84267/2012
 Fundação para a Ciência e a Tecnologia
Concluded
2015 - 2016 A Synthetic Biology based approach to construct a Lactic Acid Bacteria/artificial plasmid platform for the production of plasmid DNA and recombinant proteins
UID/BIO/04565/2013
PhD Student Fellow
Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2010/05/05 - 2013/05/04 SYNBIOBACTHER - Synthetic biology approaches to engineer "therapeutic" bacteria
info:eu-repo/grantAgreement/FCT/5876-PPCDTI/102863/PT
Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal

Universidade do Minho, Portugal

Universidade do Porto Faculdade de Engenharia, Portugal
Fundação para a Ciência e a Tecnologia
Concluded

Contract

Designation Funders
2021/01/01 - 2025/12/31 Institute for Health and Bioeconomy
LA/P/0140/2020
10.54499/LA/P/0140/2020
Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal

Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal

Universidade Nova de Lisboa, Portugal

INESC Microsistemas e Nanotecnologias, Portugal

Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal

Universidade Nova de Lisboa Associação para a Inovação e Desenvolvimento da FCT, Portugal

Unidade de Ciências Biomoleculares Aplicadas, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2018/07/01 - 2021/09/30 LactoSynt: Lactic Acid Bacteria as Cell Factories: a Synthetic Biology Approach for Plasmid DNA and Recombinant Protein Production
PTDC/BTM-SAL/28624/2017
Researcher
Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2019/01/01 - 2019/12/31 Institute for Bioengineering and Biosciences
UID/BIO/04565/2019
Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal

Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2011/01/01 - 2012/12/31 Strategic Project - LA 23 - 2011-2012
PEst-OE/EQB/LA0023/2011
Instituto de Biotecnologia e Bioengenharia, Portugal

Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal

Universidade de Lisboa Instituto Superior Técnico, Portugal

Fundação Gaspar Frutuoso, Portugal

Universidade do Minho, Portugal

Universidade do Algarve, Portugal

Fundação da Faculdade de Ciências da Universidade de Lisboa, Portugal

Universidade de Trás-os-Montes e Alto Douro, Portugal

Universidade do Minho Laboratório de Paisagens Património e Território, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
Outputs

Publications

Book chapter
  1. Duarte, Sofia O D; Monteiro, Gabriel A. "A Real-Time Quantitative PCR Protocol for the Quantification of Plasmid Copy Number in Lactococcus lactis". In Methods in Molecular Biology. 2023.
    10.1007/978-1-0716-3358-8_12
  2. Cardoso, Rafaela; Pedreira, Rita; Duarte, Sofia; Monteiro, Gabriel; Borges, Hugo; Flores-Colen, Inês. "Biocementation as Rehabilitation Technique of Porous Materials". In New Approaches to Building Pathology and Durability, 99-120. Springer Singapore, 2016.
    Published • 10.1007/978-981-10-0648-7_5
Conference paper
  1. Cláudia Viegas; Duarte, Sofia; joão lourenço; ana grenha; Fonte, Pedro. "Optimization of mPEG-PLGA nanoparticles for antibody encapsulation as a potential plataform for lung cancer treatment by inhalation". Paper presented in 6th CiiEM Congress, 2023.
  2. Francisco Dias; Borges, Inês; Duarte, Sofia O. D. ; Monteiro, Gabriel António Amaro; Cardoso, Rafaela. "Comparison of experimental techniques for biocementation of sands considering homogeneous volume distribution of precipitated calcium carbonate". Paper presented in EUNSAT2020, Lisbon, 2020.
    Accepted
  3. Albuquerque, D. C.; Cardoso, R.; Monteiro, G. A.; Duarte, S. O.; Martins, V. C.; Cardoso, S.. "Towards a portable magnetoresistive biochip for urease-based biocementation monitoring". 2019.
    10.1109/ENBENG.2019.8692505
Conference poster
  1. Cláudia Viegas; Duarte, Sofia; ana grenha; Fonte, Pedro. "Microparticles containing antibody loaded mPEG-PLGA nanoparticles for lung cancer treatment by inhalation". Paper presented in XVth Spanish-Portuguese Conference on Controlled Drug Delivery, 2024.
  2. paulo dias; josé santos; diogo antunes; Duarte, Sofia; Moreira, Leonilde; Gabriel António Amaro Monteiro. "Graph-based and Gene-based Pangenome of Lactococcus lactis and Lactococcus cremoris". Paper presented in 3rd iBB Workshop, 2023.
  3. joão saavedra; Duarte, Sofia; ana rita silva-santos; Duarte Miguel de França Teixeira dos Prazeres; ana azevedo. "Scalable purification of bacteriophages preparations". Paper presented in 3rd iBB Workshop, 2023.
  4. Cláudia Viegas; Duarte, Sofia; joão lourenço; ana grenha; Fonte, Pedro. "Optimization of spray-dried mPEG-PLGA nanoparticles for antibody loading as a potential inhalable treatment of lung cancer". Paper presented in 3rd iBB Workshop, 2023.
  5. ana brasileiro patricio; Duarte, Sofia; Fernandes-Platzgummer, Ana; Carreira, Maria Catarina; vania silverio; Fonte, Pedro. "Microfluidic platform for co-encapsulation of cells and therapeutic proteins for regenerative medicine applications". Paper presented in 3rd iBB Workshop, 2023.
  6. Duarte, Sofia; João Prata; rui borges; Fonte, Pedro. "Development of nanostructured lipid carriers for therapeutic proteins delivery". Paper presented in 3rd iBB Workshop, 2023.
  7. Dionísio, Tiago; Duarte, Sofia; vanessa machado ; joão botelho; josé joão mendes; Fonte, Pedro; pedro brandao. "Fighting endodontic infections: preliminary results on a nanodentistry approach". Paper presented in 2nd Egas Moniz One Health Symposium, 2023.
  8. Cláudia Viegas; Duarte, Sofia; joão lourenço; ana grenha; Fonte, Pedro. "Formulations of mPEG-PLGA nanoparticles dry powder for subsquent antibody loading aimed at lung cancer treatment by inhalation". Paper presented in X Congresso Iberoamericano de Ciências Farmacêuticas, 2023.
  9. ana brasileiro patricio; Duarte, Sofia; Silverio, Vania; Fonte, Pedro. "Development of microfluidic platform for the production of cell-loaded microcarriers". Paper presented in UT Austin Portugal Annual Conference 2023, 2023.
  10. Patrício, Ana; Duarte, Sofia; Silverio, Vania; Fonte, Pedro. "Fabrication of cell-loaded delivery systems for regenerative medicine applications using microfluidics". Paper presented in 3rd Chem&Biochem Students Meeting, 2023.
  11. cláudia viegas; Duarte, Sofia; joão lourenço; ana grenha; Fonte, Pedro. "Development of inhalable mPEG-PLGA nanoparticles for antibody loading as a strategy for lung cancer treatment". Paper presented in 3rd Chem&Biochem Students Meeting, 2023.
  12. joão saavedra; Duarte, Sofia; ana rita silva-santos; Duarte Miguel de França Teixeira dos Prazeres; ana margarida azevedo. "Scalable purification of bacteriophages preparations". Paper presented in 3rd Chem&Biochem Students Meeting, 2023.
  13. cláudia viegas; Duarte, Sofia; joão lourenço; ana grenha; Fonte, Pedro. "Polymeric nanoparticles for antibody loading as a potential inhalable treatment for lung cancer". Paper presented in Ciencia 2023, 2023.
  14. Paulo dias; José Miguel Miquelão Santos; Antunes, Diogo; Duarte, Sofia; Moreira, Leonilde M; Prazeres, Duarte Miguel F.; Gabriel A. Monteiro. "Comparative Genomics and Pangenomics analyses of the Lactococcus lactis and Lactococcus cremoris species: development of a new in silico pipeline". Paper presented in 2nd iBB workshop, 2022.
  15. José Miguel Miquelão Santos; Gabriel A. Monteiro; Prazeres, Duarte Miguel F.; Duarte, S.. "Deletion of the nth lactococcal nuclease gene of Lactococcus lactis subsp. lactis LMG19460 using a two-plasmid CRISPR/Cas9-based genome editing system". Paper presented in 2nd iBB workshop, 2022.
  16. Alexandre Furtado; Gabriel A. Monteiro; Duarte, S.. "A synthetic biology approach to engineering high copy number plasmids for biopharmaceutical-grade pDNA production in Lactococcus lactis". Paper presented in 2nd iBB workshop, 2022.
  17. Duarte, S.; Bruno Costa; Paulo Dias; Moreira, Leonilde M; Prazeres, Duarte Miguel F.; Pedro pinheiro; Gonçalo justino; Gabriel A. Monteiro. "Exometabolome analysis of Lactococcus lactis LMG19460 – focus on the characterization of a novel ferulic acid pathway". Paper presented in 3º Bioiberoamerica, Ibero-American Congress on Biotechnology, 2022.
  18. Duarte, Sofia O. D. ; Bruno Costa; 031C-B4B2-0B2E; Moreira, Leonilde M; Prazeres, Duarte Miguel F.; Pinheiro, Pedro F.; Justino, Gonçalo C.; Monteiro, Gabriel António Amaro. "Characterization of a novel ferulic acid pathway in Lactococcus lactis". Paper presented in Microbiotec21, 2021.
  19. 031C-B4B2-0B2E; 3510-7BD2-BCD1; Antunes, Diogo; Duarte, Sofia O. D. ; Moreira, Leonilde M; Monteiro, Gabriel António Amaro. "Genome Sequence and Annotation of Lactococcus lactis Strain LMG 19460 and Novel Strains IST-LL01 and IST-LL02". Paper presented in Microbiotec21, 2021.
  20. 031C-B4B2-0B2E; Diogo de Lucena; Bruno Costa; Duarte, Sofia O. D. ; Pinheiro, Pedro F.; Justino, Gonçalo C.; Moreira, Leonilde M; Monteiro, Gabriel António Amaro. "Reconstruction of the Metabolic Network of Lactococcus lactis strains IL1403 and LMG 19460". Paper presented in Microbiotec21, 2021.
  21. Silva, Bruno; Duarte, Sofia O. D. ; Moreira, Leonilde M; Monteiro, Gabriel António Amaro. "Screening of Lactic Acid Bacteria strains for Electrotransformation Efficiency". Paper presented in Microbiotec21, 2021.
  22. 3510-7BD2-BCD1; Duarte, Sofia O. D. ; Prazeres, Duarte M. F.; Monteiro, Gabriel António Amaro. "Deletion of the nuclease gene nth leads to a decrease in plasmid DNA yields in Lactococcus lactis subsp. lactis LMG19460". Paper presented in 13th International Symposium on Lactic Acid Bacteria, 2021.
  23. Duarte, Sofia O. D. ; 031C-B4B2-0B2E; Moreira, Leonilde M; Prazeres, Duarte Miguel F.; Pinheiro, Pedro F.; Justino, Gonçalo C.; Monteiro, Gabriel António Amaro. "Identification of the ferulic acid metabolism in Lactococcus lactis". Paper presented in 13th International Symposium on Lactic Acid Bacteria, 2021.
  24. 031C-B4B2-0B2E; Diogo de Lucena; Duarte, Sofia O. D. ; Pinheiro, Pedro F.; Moreira, Leonilde M; Monteiro, Gabriel António Amaro. "Reconstruction of the Metabolic Network of Lactococcus lactis LMG 19460". Paper presented in 13th International Symposium on Lactic Acid Bacteria, 2021.
  25. Silva, Bruno; Duarte, Sofia O. D. ; Monteiro, Gabriel António Amaro. "A high copy number plasmid with a high level of heterologous protein expression for Lactic Acid Bacteria". Paper presented in 13th International Symposium on Lactic Acid Bacteria, 2021.
  26. Duarte, Sofia O. D. ; Monteiro, Gabriel António Amaro. "Redesign of Lactococcus lactis genome for increasing pDNA and recombinant protein production using a CRISPR/Cas9 strategy". Paper presented in 2021 Synthetic Biology: Engineering, Evolution & Design (SEED), 2021.
  27. 3510-7BD2-BCD1; Duarte, Sofia O. D. ; Prazeres, Duarte M. F.; Monteiro, Gabriel António Amaro. "Application of a CRISPR/Cas9-based genome editing system for improved biopharmaceutical grade plasmid DNA and recombinant protein production in Lactococcus lactis". Paper presented in 5th Applied Synthetic Biology in Europe, 2020.
  28. Santos, José; Monteiro, Gabriel António Amaro; Prazeres, Miguel; Duarte, Sofia Oliveira Dias. "Application of a CRISPR/Cas9-based genome editing system for improved biopharmaceutical grade plasmid DNA and recombinant protein yields in Lactococcus lactis". Paper presented in ASBE V - Applied Synthetic Biology in Europe, 2020.
  29. Silva, Bruno; Duarte, Sofia O. D. ; Monteiro, Gabriel António Amaro. "A high copy number plasmid with a high level of heterologous protein expression for Lactic Acid Bacteria". Paper presented in 5th Applied Synthetic Biotechnology in Europe, 2020.
  30. Pires, Rita; Monteiro, Gabriel; Prazeres, Miguel; Duarte, Sofia. "Genome editing for improvement of food-grade lactic acid bacteria". Paper presented in Microbiotec’19 Congress of Microbiology and Biotechnology 2019, 2019.
  31. Sancho, Stefany; Monteiro, Gabriel; Prazeres, Miguel; Moreira, Leonilde; Duarte, Sofia. "Screening of Lactic Acid Bacteria strains for biopharmaceutical-grade plasmid and recombinant protein production". Paper presented in Microbiotec’19 Congress of Microbiology and Biotechnology 2019, 2019.
  32. Santos, José; Monteiro, Gabriel; Prazeres, Duarte; Duarte, Sofia. "Adaptation of a one-plasmid genome editing system for increased production of biopharmaceutical grade recombinant molecules in Lactococcus lactis". Paper presented in Microbiotec’19 Congress of Microbiology and Biotechnology 2019, 2019.
  33. Duarte, Sofia O. D. ; Martins, Maria C.; Andrade, Sílvia M.; Prazeres, Duarte M. F.; Monteiro, Gabriel A.. "Engineering high copy number plasmids for pharmaceutical-grade pDNA production in Lactococcus lactis". Paper presented in BIOTEC 2019, Congreso Nacional de Biotecnologia, 2019.
  34. Albuquerque, D. C.; Cardoso, R.; Monteiro, G. A.; Duarte, S. O.; Martins, V. C.; Cardoso, S.. "Towards a portable magnetoresistive biochip for urease-based biocementation monitoring". Paper presented in 6th IEEE Portuguese Meeting on Bioengineering (ENBENG 2019), 2019.
  35. Duarte, Sofia O. D. ; Alves, Cláudia P. A.; Martins, Maria C.; Cipriano, Diana; Silva, Inês N.; Moreira, Leonilde M.; Prazeres, Duarte Miguel F.; Monteiro, Gabriel A.. "Is increased plasmid DNA production in the Escherichia coli pgi knockout strain GALG20 linked to unintentional genomic deletions?". Paper presented in Microbiotec’15 Congress of Microbiology and Biotechnology 2015, 2015.
  36. Martins, Maria C.; Duarte, Sofia O. D. ; Andrade, Sílvia; Santos, José A.; Prazeres, Duarte Miguel F.; Monteiro, Gabriel A.. "Redesign of Lactic Acid Bacteria for plasmid DNA production". Paper presented in Microbiotec’15 Congress of Microbiology and Biotechnology 2015, 2015.
  37. Duarte, S.; Rodrigues, L.; Monteiro, G.. "Heterologous production of curcumin by probiotic lactic acid bacteria towards gastrointestinal cancer therapy". Paper presented in E3 Forum-Education, Employment & Entrepreneurship Conference, 2014.
  38. Duarte, S. O. D.; Paulo, J.; Machado, D.; Rocha, I.; Mergulhão, F. J. M.; Rodrigues, L. R; Monteiro, G. A.. "A synthetic biology approach to engineer "therapeutic" bacteria". Paper presented in MicroBiotec’13 Congress of Microbiology and Biotechnology 2013, 2013.
  39. Magalhães, Salomé; Duarte, Sofia; Monteiro, Gabriel A.; Prieto, Manuel; Fernandes, Fábio. "Trafficking Studies of plasmid DNA through Förster Resonance Energy Transfer". Paper presented in MicroBiotec’13 Congress of Microbiology and Biotechnology 2013, 2013.
  40. Magalhães, Salomé; Duarte, Sofia; Monteiro, Gabriel A.; Prieto, Manuel; Fernandes, Fábio. "Plasmid DNA delivery followed through Förster Resonance Energy Transfer". Paper presented in 9th EBSA European Biophysics Congress, 2013.
  41. Duarte, Sofia; Rodrigues, Lígia; Monteiro, Gabriel A.. "Heterologous production of curcumin by probiotic lactic acid bacteria towards gastrointestinal cancer therapy". Paper presented in E3 Forum-Education, Employment & Entrepreneurship Conference, 2013.
Journal article
  1. Mahesh Kumar; Tarun Virmani; Girish Kumar; Rohitas Deshmukh; Ashwani Sharma; Sofia Duarte; Pedro Brandão; Pedro Fonte. "Nanocarriers in Tuberculosis Treatment: Challenges and Delivery Strategies". Pharmaceuticals (2023): https://doi.org/10.3390/ph16101360.
    10.3390/ph16101360
  2. Cláudia Viegas; Ana B. Patrício; João Prata; Leonor Fonseca; Ana S. Macedo; Sofia O. D. Duarte; Pedro Fonte. "Advances in Pancreatic Cancer Treatment by Nano-Based Drug Delivery Systems". Pharmaceutics (2023): https://doi.org/10.3390/pharmaceutics15092363.
    10.3390/pharmaceutics15092363
  3. Cardoso, Rafaela; Borges, Inês; Joana Vieira Paulino; Duarte, Sofia O. D. ; Gabriel A. Monteiro. "Interactions between clay minerals, bacteria growth and urease activity on biocementation of soils". Applied Clay Science (2023):
    Open access • Accepted
  4. Gabriel A. Monteiro; Sofia O. D. Duarte. "The Effect of Recombinant Protein Production in Lactococcus lactis Transcriptome and Proteome". Microorganisms 10 2 (2022): 267-267. https://doi.org/10.3390/microorganisms10020267.
    10.3390/microorganisms10020267
  5. Cardoso, Rafaela; Arbabzadeh, Emad; de Lima, Jonathan Tenório; Flores-Colen, Inês; Pereira, Manuel F.C.; Costa e Silva, Matilde; Duarte, Sofia O.D.; Monteiro, Gabriel A.. "The influence of stone joints width and roughness on the efficiency of biocementation sealing". Construction and Building Materials 283 (2021): 122743. http://dx.doi.org/10.1016/j.conbuildmat.2021.122743.
    10.1016/j.conbuildmat.2021.122743
  6. Sofia Duarte; Gabriel A. Monteiro. "Plasmid Replicons for the Production of Pharmaceutical-Grade pDNA, Proteins and Antigens by Lactococcus lactis Cell Factories". International Journal of Molecular Sciences (2021): https://www.mdpi.com/1422-0067/22/3/1379.
    Published • 10.3390/ijms22031379
  7. Cardoso, Rafaela; Pedreira, Rita; Duarte, Sofia O.D.; Monteiro, Gabriel A.. "About calcium carbonate precipitation on sand biocementation". Engineering Geology 271 (2020): 105612. http://dx.doi.org/10.1016/j.enggeo.2020.105612.
    10.1016/j.enggeo.2020.105612
  8. Monteiro, G. A.; Duarte, S. O.; Martins, M. C.; Andrade, S. M.; Prazeres, D. M.. "High copy number plasmid engineering for biopharmaceutical-grade pDNA production in Lactococcus lactis". Journal of Biotechnology 305 (2019): S23-S24. http://dx.doi.org/10.1016/j.jbiotec.2019.05.092.
    Published • 10.1016/j.jbiotec.2019.05.092
  9. Felício, Filipe; Silverio, Vania; Duarte, Sofia; Galvão, Ana; Monteiro, Gabriel; Cardoso, Susana. "Preliminary tests on a microfluidic device to study pore clogging during biocementation". E3S Web of Conferences 92 (2019): 11018. http://dx.doi.org/10.1051/e3sconf/20199211018.
    Open access • Published • 10.1051/e3sconf/20199211018
  10. Duarte, Sofia O. D. ; Martins, Maria C.; Andrade, Sílvia M.; Prazeres, Duarte M. F.; Monteiro, Gabriel A.. "Plasmid Copy Number of pTRKH3 in Lactococcus lactis is Increased by Modification of the repDE Ribosome-Binding Site". Biotechnology Journal 14 8 (2019): 1800587. http://dx.doi.org/10.1002/biot.201800587.
    Open access • Published • 10.1002/biot.201800587
  11. Cardoso, Rafaela; Pires, Inês; Duarte, Sofia O. D. ; Monteiro, Gabriel A.. "Effects of clay's chemical interactions on biocementation". Applied Clay Science 156 (2018): 96-103. http://dx.doi.org/10.1016/j.clay.2018.01.035.
    Published • 10.1016/j.clay.2018.01.035
  12. Silva IN; Duarte S; Moreira LM; Monteiro GA. "Draft Genome Sequence of the Plasmid-Free Lactococcus lactis subsp. lactis Strain LMG 19460.". Genome announcements (2017): http://europepmc.org/abstract/med/28428305.
    Published • 10.1128/genomeA.00210-17
  13. Alves, Cláudia P. A.; Duarte, Sofia O. D. ; Monteiro, Gabriel A.; Prazeres, Duarte Miguel F.. "Use of an Escherichia coli pgi knockout strain as a plasmid producer". BioPharm International 28 2 (2016): 38-42. https://www.biopharminternational.com/use-e-coli-pgi-knockout-strain-plasmid-producer.
    Open access • Published
  14. Magalhães, Salomé; Duarte, Sofia; Monteiro, Gabriel A.; Fernandes, Fábio. "Quantitative Evaluation of DNA Dissociation from Liposome Carriers and DNA Escape from Endosomes During Lipid-Mediated Gene Delivery". Human Gene Therapy Methods 25 5 (2014): 303-313. http://dx.doi.org/10.1089/hgtb.2014.080.
    Published • 10.1089/hgtb.2014.080
Thesis / Dissertation
  1. Duarte, Sofia. "Lactic acid bacteria as cell factories: A synthetic biology approach for plasmid DNA and recombinant protein production". PhD, Universidade de Lisboa Instituto Superior Técnico, 2018.
  2. Duarte, Sofia de Oliveira Dias; Monteiro, Gabriel António Amaro; Tenreiro, Ana Maria Moura Pires de Andrade, 1952-. "The role of calcium in Saccharomyces sp. response to ethanol stress". Master, Universidade de Lisboa Faculdade de Ciências, 2011. http://hdl.handle.net/10451/4029.

Other

Other output
  1. One-plasmid based CRISPR-Cas9 editing for gene deletion in Lactococcus lactis. 2021. José Miguel Miquelão Santos; Gabriel António Amaro Monteiro; Duarte Miguel de França Teixeira dos Prazeres; Sofia de Oliveira Dias Duarte. https://doi.org/10.21203/rs.3.rs-306012/v1.
    10.21203/rs.3.rs-306012/v1
  2. Cover Picture: Biotechnology Journal 8/2019. Cover Picture: Biotechnology Journal 8/2019. 2019. Duarte, Sofia O. D. ; Martins, Maria C.; Andrade, Sílvia M.; Prazeres, Duarte M. F.; Monteiro, Gabriel A.. https://onlinelibrary.wiley.com/doi/abs/10.1002/biot.201970081.
    10.1002/biot.201970081
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2024/01/26 Optimizing nanostructured lipid carriers as therapeutic protein delivery systems IX Clinical Biochemistry Workshop
(Portugal)
2024/01 Microparticles containing antibody encapsulated in mPEG-PLGA nanoparticles intended for lung cancer treatment by inhalation IX Clinical Biochemistry Workshop
(Portugal)
2023/07/13 Scalable purification of bacteriophages preparations 3rd Chem&Biochem Students Meeting
(Portugal)
2023/07/07 Optimization of mPEG-PLGA nanoparticles for antibody encapsulation as a potential plataform for lung cancer treatment by inhalation 6th CiiEM Congress
Egas Moniz School of Health & Science (Portugal)
2023/07/05 Microfluidic fabrication of cell-loaded delivery systems for regenerative medicine applications 6th CiiEM Congress
Egas Moniz School of Health & Science (Portugal)
2022/12/19 Lactococcus lactis as cell factories for the advanced manufacturing of biopharmaceuticals i4HB Kick-off meeting
(Portugal)
2022/09/23 A synthetic biology approach to engineering high copy number plasmids for biopharmaceutical-grade pDNA production in Lactococcus lactis 2nd iBB workshop
(Portugal)
2022/04/07 Engineering of a high copy number plasmid for a high level of heterologous protein expression 3º Bioiberoamerica, Ibero-American Congress on Biotechnology
(Portugal)
2022/04/07 Development of a multiple plasmid system, CRISPR/Cas9-based, for genome editing of Lactococcus lactis subsp. lactis LMG19460 3º Bioiberoamerica, Ibero-American Congress on Biotechnology
(Portugal)
2022/04/07 Redesign of Lactococcus lactis genome for increasing pDNA and recombinant protein production using a CRISPR/Cas9 strategy 3º Bioiberoamerica, Ibero-American Congress on Biotechnology
(Portugal)
2021/11/26 Targeted removal of the nth nuclease gene from Lactococcus lactis subsp. lactis LMG19460 and its effects on plasmid DNA yield Microbiotec21
(Portugal)
2021/11/25 A sustainable alternative for cheese production – a synthetic biology approach for chymosin production using recombinant Lactic Acid Bacteria Microbiotec21
(Portugal)
2020/11/02 Increasing pDNA and recombinant protein production by Lactococcus lactis using a CRISPR/Cas9 strategy for genome redesign 5th Applied Synthetic Biotechnology in Europe
(Netherlands)
2019/09 Increasing plasmid copy number of PTRKH3 in Lactococcus lactis for biopharmaceutical-grade pDNA production ECCE12/ECAB5 (European Congress of Chemical Engineering/ European Congress of Applied Biotechnology)
(Florence, Italy)
2019/06 Preliminary tests on a microfluidic device to study pore clogging during biocementation 7th International Symposium on Deformation Characteristics of Geomaterials
(Glasgow, United Kingdom)
2019/04 High copy number plasmid engineering for biopharmaceutical-grade pDNA production in Lactococcus lactis European Biotechnology Congress 2019
(Valencia, Spain)
2018/09/12 A synthetic biology approach for a safe and cost-effective pDNA and recombinant protein production using L. lactis ESBES 2018, 12th European Symposium on Biochemical Engineering Sciences
(Lisbon, Portugal)
2016/06/06 Engineering Lactic Acid Bacteria to improve plasmid DNA and protein production BioIberoAmerica 2016
(Salamanca, Spain)
2016/06 Melhoramento de um solo arenoso através de bio-cimentação [Improvement of a sandy soil through biocementation] 15CNG/8CLBG (15º Congresso Nacional de Geotecnia - 8º Congresso Luso-Brasileiro de Geotecnia 2016)
(Porto, Portugal)
2015/03 Biocementation as rehabilitation technique ISBP2015 1st International Symposium on Building Pathology
(Porto, Portugal)

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2022/09 - 2023/12 Scalable production of bacteriophages preparations
Co-supervisor
2021/09/01 - 2022/10 A sustainable alternative for cheese production – a synthetic biology approach for chymosin production using recombinant Lactic Acid Bacteria
Supervisor of Alexandre Furtado
 Microbiologia Aplicada (Master)
Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
2021/09/01 - 2022/10 CRISPR/Cas9-based genome editing of Lactic Acid Bacteria for the improvement of curcumin production
Supervisor of Bruno Costa
 Bioengenharia e Nanossistemas (Master)
Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
2021/02 - 2022/07 Evolution of the endonuclease nth in Lactobacillales species
Co-supervisor
2022/02/01 - 2022/04/01 A synthetic biology approach for engineering a reporter plasmid for testing Lactococcus lactis knockout strains
Supervisor
Universidade NOVA de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2018/09/01 - 2019/12 Screening of Lactic Acid Bacteria strains for biopharmaceutical-grade plasmid and recombinant protein production
Supervisor of Stefany Dias Sancho
 Microbiologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2018/07/01 - 2019/11 CRISPR/Cas9-based genome editing of lactic acid bacteria for the improvement of biopharmaceutical-grade plasmid and recombinant protein production
Supervisor of José Miguel Miquelão Santos
 Microbiologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2018/07/01 - 2019/11 Lactic acid bacteria as producers of pharmaceutical-grade molecules: genetic engineering tools for improvement of Lactococcus lactis strains
Supervisor of Rita Pereira Coutinho Relvas Pires
 Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal

Event organisation

Event name
Type of event (Role) 		Institution / Organization
2018/07/01 - 2018/09/12 Participation in the Organizing Committee of the ESBES2018 symphosium. (2018/09/09 - 2018/09/12)
Symposium (Member of the Organising Committee)
 Universidade de Lisboa Instituto Superior Técnico, Portugal

Event participation

Activity description
Type of event 		Event name
Institution / Organization
2023 - 2023 Course “Advanced course on biomedical imaging”, organized by UTAustin Portugal
Other
2023 - 2023 Course “Utilização de extintores”, organized by Instituto Superior Técnico
Other
2023 - 2023 Course “Manual de segurança para laboratórios”, organized by Instituto Superior Técnico
Other
2023 - 2023 Course “Revisão sistemática da literatura”, organized by Instituto Superior Técnico
Other
2023 - 2023 Online symposium “The Landscape of Cancer Research”, organized by Technology Networks
Symposium
2023 - 2023 Webinar “Discover New Ways To Increase Your Throughput for Metabolic Stability Assays”, organized by Technology Networks
Seminar
2023 - 2023 Webinar “Quality considerations for plasmid DNA as a starting material for cell and gene therapies”, organized by RegMedNet
Seminar
2023 - 2023 Webinar “Powder Caking: Understanding Different Caking Behaviors and the Influence of Humidity”, organized by MicroMeritics
Seminar

Jury of academic degree

Topic
Role 		Candidate name (Type of degree)
Institution / Organization
2023/12/12 Purification of plasmid DNA using steric exclusion chromatography
(Thesis) Main arguer
 Mariana Silva (Master)
2023/11/29 Phage therapy to treat bacterial infections in Hidradenitis suppurativa patients
(Thesis) Main arguer
 Madalena Almeida (Master)
2023/11/24 Nanotecnologia aplicada à regeneração de feridas
(Thesis) Main arguer
 Ana Rita Amaro (Master)
2023/11/17 Improving strategies to develop anti-Candida treatments based on the vaginal species L. gasseri: from molecular approaches to physiological analyses
(Thesis) Main arguer
 Gabriela Fontebasso (Master)
2022/10 Quality control of phage formulations during GMP manufacturing
(Thesis) Main arguer
 Catarina Silva (Master)
2021/11/22 Lactic acid fermentation of industrial residues from the macroalga Gelidium corneum towards the improvement of protein content and nutritional value
(Thesis) Main arguer
 Matilde Narciso (Master)
2021/07/15 Cloning And Expression Of Human Recombinant Proteins - Initial steps for an enzymatic reactor to mimic drug metabolism
(Thesis) Main arguer
 Josimar Cruz (Master)

Consulting

Activity description Institution / Organization
2020 - 2021 Co-management of the logistics of the IST-COVID laboratory, which provided PCR tests for SARS-CoV-2 detection to the entire IST academic community

Journal scientific committee

Journal title (ISSN) Publisher
2023 - Current Pharmaceutical Patent Analyst
2023 - Current JSMCentral
2022 - Current Microbial Cell Factories
2022 - Current Journal of Chromatography B
2021/04 - Current Scientific Reports Nature
2020/02/25 - Current Marine Georesources & Geotechnology
Distinctions

Other distinction

2011 Menção honrosa por melhor classificação no 1º ano do mestrado em Microbiologia Aplicada
Universidade de Lisboa Faculdade de Ciências, Portugal
2010 Menção honrosa por melhor classificação no 3º ano da licenciatura em Biologia
Universidade de Lisboa Faculdade de Ciências, Portugal
2009 Menção honrosa por melhor classificação no 2º ano da licenciatura em Biologia
Universidade de Lisboa Faculdade de Ciências, Portugal
2008 Menção honrosa por melhor classificação no 1º ano da licenciatura em Biologia
Fundação da Faculdade de Ciências da Universidade de Lisboa, Portugal