???global.info.a_carregar???
Carlos MGA Fontes, was born in 9 Decembre 1968 in Lisbon, Portugal. Carlos Fontes received his DScAgr degree in 1991 from the Technical University of Lisbon. Working at Prof. Harry J. Gilbert laboratory, he was awarded the MSc (1992) PhD (1996) degrees, by the University of Newcastle upon Tyne, United Kingdom. After a Pos-Doc at Prof. Luís Ferreira group (University of Lisbon), he become professor at the Faculty of Veterinary Medicine (FMV) at the University of Lisbon, where he developed cutting edge research in structural and molecular biology of plant cell wall degrading protein complexes (also termed cellulosomes), including the study of structure and function relationships in different Carbohydrate Active enZymes (CAZymes) and Carbohydrate Binding Modules (CBMs). He became Full Professor in 2015 and left the University in September 2021 to become the Chief Scientific Officer of NZYTech genes & enzymes (www.nzytech.com), the company he founded in 2008. NZYTech explores the commercial applications related with the use of recombinant enzymes and molecular kits, in particular in the life science and molecular diagnostic fields. Carlos Fontes has supervised 17 PhD students and is co-author of more than 200 papers published in international refereed journals. Relevant publications: 1. Ana L. Carvalho, Fernando M.V. Dias, José A.M. Prates, Tibor Nagy, Harry J. Gilbert, Gideon J. Davies, Luís M.A. Ferreira, Maria J. Romao & Carlos M.G.A. Fontes (2003) Cellulosome assembly revealed by the crystal structure of the cohesin-dockerin complex. Procedings of the National Academy of Sciences United States of America 100, 13809-13814. 2. Ana L. Carvalho, Fernando M.V. Dias, Tibor Nagy, José A.M. Prates, Mark R. Proctor, Nicola Smith, Edward A. Bayer, Gideon J. Davies, Luís M.A. Ferreira, Maria João Romão, Carlos M.G.A. Fontes e Harry J. Gilbert & (2007). Evidence for a dual binding mode of dockerin modules to cohesins. Procedings of the National Academy of Sciences United States of America 104, 3089-3094. 3. Cedric Montanier, Alicia L. vanBueren, Claire Dumon, James E. Flint, Marcia A. Correia, Jose A.M. Prates, Susan Firbank, Richard J. Lewis, Gilles G. Grondin, Mariana G. Ghinet, Tracey M. Gloster, Cecile Herve, Paul Knox, Brian G. Talbot, Johan P. Turkenburg, Janne Kerovuo, Ryszard Brzezibski, Carlos M.G.A. Fontes, Gideon J. Davies, Alisdair B. Boraston & Harry J. Gilbert (2009). Evidence that family 35 carbohydrate binding modules display conserved specificity but divergent function. Procedings of the National Academy of Sciences United States of America 106, 3065-3070. 4. Carlos M.G.A. Fontes & Harry J. Gilbert (2010). Cellulosomes: highly efficient nanomachines designed to deconstruct plant cell wall complex carbohydrates. Annual Reviews in Bichemistry 79, 655-81. 5. Brás JL, Cartmell A, Carvalho AL, Verzé G, Bayer EA, Vazana Y, Correia MA, Prates JA, Ratnaparkhe S, Boraston AB, Romão MJ, Fontes CMGA & Gilbert HJ (2011). Structural insights into a unique cellulase fold and mechanism of cellulose hydrolysis. Procedings of the National Academy of Sciences United States of America 108, 5237-5242. 6. Luís AS, Venditto I, Prates JA, Ferrieira LM, Temple MJ, Rogowski A, Baslé A, Xue J, Knox JP, Najmudin S, Fontes CM & Gilbert HJ (2013). Understanding how non-catalytic carbohydrate binding modules can display specificity for xyloglucan. The Journal of Biological Chemistry 288, 4799-809. 7. Venditto I, Luis AS, Rydahl M, Schückel J, Fernandes VO, Vidal-Melgosa S, Bule P, Goyal A, Pires VM, Dourado CG, Ferreira LM, Coutinho PM, Henrissat B, Knox JP, Baslé A, Najmudin S, Gilbert HJ, Willats WG, Fontes CMGA (2016). Proceedings of the National Academy of Sciences USA 113, 7136-7141.
Read full resume ↓
Identification

Personal identification

Full name
Carlos MGA Fontes

Citation names

  • Carlos MGA Fontes
  • Fontes, CM
  • Fontes, CMGA

Author identifiers

Ciência ID
C01A-FCB3-7F99
ORCID iD
0000-0002-1219-9753

Email addresses

  • cafontes@fmv.ulisboa.pt (Professional)
  • carlos.fontes@nzytech.com (Professional)

Telephones

Fax
  • 213643514 (Professional)

Addresses

  • NZYTech genes & enzymes, Estrada do Paço do Lumiar, Campus do Lumiar, Edifício E – 1º andar, 1649-038, Lisboa, Lisboa, Portugal (Professional)

Websites

Languages

Language Speaking Reading Writing Listening Peer-review
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Affiliation

Teaching in Higher Education

Category
Host institution 		Employer
2015/12/09 - 2021/08/31 Full Professor (University Teacher) Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal

Others

Category
Host institution 		Employer
2021/09/01 - Current Diretor Científico NZYTech Lda, Portugal
Projects

Grant

Designation Funders
2016/01 - 2018/12 Molecular determinants of bacterial cellulosome diversity in different ecological niches
grant.Provided by PTCRIS: 144587
 Fundação para a Ciência e a Tecnologia
2013/07 - 2016/06 Modern Structural Biology: Resources for the advancement of in-house X-ray Crystallography
grant.Provided by PTCRIS: 125363
 Fundação para a Ciência e a Tecnologia
2013/07 - 2014/12 Development of cohesin- microarrays for determining cohesin-dockerin specificities of novel cellulolytic species
grant.Provided by PTCRIS: 126085
 Fundação para a Ciência e a Tecnologia
2013/06 - 2014/11 An Innovative Recombinant Hit-Maximization Strategy to Unravel the Structure of Cohesin-Dockerin Complexes of Bacteroides cellulosolvens and Acetivibrio cellulolyticus
grant.Provided by PTCRIS: 129521
 Fundação para a Ciência e a Tecnologia
2011/03 - 2014/08 A carbohydrate microarray platform for discovery of ligands in polysaccharides recognised by microbial carbohydrate-binding modules.
grant.Provided by PTCRIS: 112537
 Fundação para a Ciência e a Tecnologia
2010/03 - 2013/11 Improving fat partioning and nutritional quality in pigs: developing feeding strategies and providing insights into genetic and metabolic mechanisms
grant.Provided by PTCRIS: 99210
 Fundação para a Ciência e a Tecnologia
2010/01 - 2013/10 Molecular mechanisms affecting the efficacy of feed enzymes: endogenous beta-glucanases, protein-inhibitors and mini-cellulosomes
grant.Provided by PTCRIS: 103942
 Fundação para a Ciência e a Tecnologia
2010/03 - 2013/08 Prion gene complex: insights on ram fertility
grant.Provided by PTCRIS: 98607
 Fundação para a Ciência e a Tecnologia
2010/01 - 2013/06 The Cellulosome assembly: structure, function and specificity of new cellulases and supramolecular complexes
grant.Provided by PTCRIS: 100359
 Fundação para a Ciência e a Tecnologia
2010/01 - 2013/06 Structure and function of novel Glycoside Hydrolases and Carbohydrate-Binding Modules of Clostridium thermocellum cellulosome
grant.Provided by PTCRIS: 103980
 Fundação para a Ciência e a Tecnologia
2009/01 - 2011/12 Structure and function of novel carbohydrate-binding modules and cohesin-dockerin protein complexes from Clostridium thermocellum
grant.Provided by PTCRIS: 69732
 Fundação para a Ciência e a Tecnologia
2007/10 - 2011/01 An integrated study of lipid composition and its genetic regulation in bovine meats with Protected Designation of Origin (PDO)
grant.Provided by PTCRIS: 66114
 Fundação para a Ciência e a Tecnologia
2007/10 - 2010/12 Rational utilization of cellulases and hemicellulases for improving meat quality and the nutritive value of cereal-based diets for poultry
grant.Provided by PTCRIS: 69329
 Fundação para a Ciência e a Tecnologia
2007/01 - 2010/01 Doppel gene polymorphisms characterization according to scrapie susceptibility genotypes in Portuguese sheep breeds and their impact on male fertility.
grant.Provided by PTCRIS: 82560
 Fundação para a Ciência e a Tecnologia
2007/01 - 2009/04 Molecular determinants of ligand specificity in carbohydrate-binding modules and cohesin-dockerin complexes
grant.Provided by PTCRIS: 82210
 Fundação para a Ciência e a Tecnologia
2007/01 - 2009/01 Using recombinant enzymes for improving broiler performance and meat quality
grant.Provided by PTCRIS: 82499
 Fundação para a Ciência e a Tecnologia
2005/06 - 2008/05 Doppel gene polymorphisms characterization according to scrapie susceptibility genotypes in Portuguese sheep breeds and their impact on male fertility.
grant.Provided by PTCRIS: 57148
 Fundação para a Ciência e a Tecnologia
2005/07 - 2007/12 Molecular determinants of ligand specificity in carbohydrate-binding modules and cohesin-dockerin complexes
grant.Provided by PTCRIS: 59118
 Fundação para a Ciência e a Tecnologia
2005/07 - 2007/12 Using recombinant enzymes for improving broiler performance and meat quality
grant.Provided by PTCRIS: 61162
 Fundação para a Ciência e a Tecnologia
Outputs

Publications

Book
  1. Sequeira, A.F.; Brás, J.L.A.; Fernandes, V.O.; Guerreiro, C.I.P.D.; Vincentelli, R.; Fontes, C.M.G.A.. A novel platform for high-throughput gene synthesis to maximize recombinant expression in Escherichia coli. 2017.
    10.1007/978-1-4939-7060-5_7
  2. Stern, J.; Artzi, L.; Moraïs, S.; Fontes, C.M.G.A.; Bayer, E.A.. Carbohydrate depolymerization by intricate cellulosomal systems. 2017.
    10.1007/978-1-4939-6899-2_8
  3. Brás, J.L.A.; Carvalho, A.L.; Viegas, A.; Najmudin, S.; Alves, V.D.; Prates, J.A.M.; Ferreira, L.M.A.; et al. Escherichia coli expression, purification, crystallization, and structure determination of bacterial cohesin-dockerin complexes. 2012.
    10.1016/B978-0-12-415931-0.00021-5
  4. Fontes, C.M.G.A.; Gilbert, H.J.. Cellulosomes: Highly efficient nanomachines designed to deconstruct plant cell wall complex carbohydrates. 2010.
    10.1146/annurev-biochem-091208-085603
  5. Ali, S.; Hall, J.; Soole, K.L.; Fontes, C.M.G.A.; Hazlewood, G.P.; Hirst, B.H.; Gilbert, H.J.. Targeted expression of microbial cellulases in transgenic animals. 1995.
    10.1016/S0921-0423(06)80111-5
Book chapter
  1. Alves, Victor D.; Fontes, Carlos M. G. A.; Bule, Pedro. "Cellulosomes: Highly Efficient Cellulolytic Complexes". In Macromolecular Protein Complexes III: Structure and Function, 323-354. Springer International Publishing, 2020.
    10.1007/978-3-030-58971-4_9
  2. Duhoo, Y.; Girault, V.; Turchetto, J.; Ramond, L.; Durbesson, F.; Fourquet, P.; Nominé, Y.; et al. "High-throughput production of a new library of human single and tandem PDZ domains allows quantitative PDZ-peptide interaction screening through high-throughput holdup assay". 439-476. 2019.
    10.1007/978-1-4939-9624-7_21
  3. Duhoo, Y.; Sequeira, A.F.; Saez, N.J.; Turchetto, J.; Ramond, L.; Peysson, F.; Brás, J.L.A.; et al. "High-throughput production of oxidized animal toxins in escherichia coli". 165-190. 2019.
    10.1007/978-1-4939-9624-7_7
Journal article
  1. Coelho, Diogo; Alfaia, Cristina M.; Lopes, Paula A.; Pestana, José M.; Costa, Mónica M.; Pinto, Rui M.A.; Almeida, João M.; et al. "Impact of Chlorella vulgaris as feed ingredient and carbohydrases on the health status and hepatic lipid metabolism of finishing pigs". Research in Veterinary Science 144 (2022): 44-53. http://dx.doi.org/10.1016/j.rvsc.2022.01.008.
    10.1016/j.rvsc.2022.01.008
  2. Costa, Mónica M.; Pio, Luís B.; Bule, Pedro; Cardoso, Vânia A.; Duarte, Marlene; Alfaia, Cristina M.; Coelho, Diogo F.; et al. "Recalcitrant cell wall of Ulva lactuca seaweed is degraded by a single ulvan lyase from family 25 of polysaccharide lyases". Animal Nutrition (2022): http://dx.doi.org/10.1016/j.aninu.2022.01.004.
    10.1016/j.aninu.2022.01.004
  3. Correia, Viviana G.; Trovão, Filipa; Pinheiro, Benedita A.; Brás, Joana L. A.; Silva, Lisete M.; Nunes, Cláudia; Coimbra, Manuel A.; et al. "Mapping Molecular Recognition of ß1,3-1,4-Glucans by a Surface Glycan-Binding Protein from the Human Gut Symbiont Bacteroides ovatus". Microbiology Spectrum 9 3 (2021): http://dx.doi.org/10.1128/spectrum.01826-21.
    10.1128/spectrum.01826-21
  4. Mondal, Sunetra; Thakur, Abhijeet; Fontes, Carlos M. G. A.; Goyal, Arun. "A trimodular family 16 glycoside hydrolase from the cellulosome of Ruminococcus flavefaciens displays highly specific licheninase (EC 3.2.1.73) activity". Microbiology 167 7 (2021): http://dx.doi.org/10.1099/mic.0.001055.
    10.1099/mic.0.001055
  5. Costa, Mónica; Pio, Luís; Bule, Pedro; Cardoso, Vânia; Alfaia, Cristina M.; Coelho, Diogo; Brás, Joana; Fontes, Carlos M. G. A.; Prates, José A. M.. "An individual alginate lyase is effective in the disruption of Laminaria digitata recalcitrant cell wall". Scientific Reports 11 1 (2021): http://dx.doi.org/10.1038/s41598-021-89278-1.
    10.1038/s41598-021-89278-1
  6. Ahmed, Jebin; Kumar, Krishan; Sharma, Kedar; Fontes, Carlos M. G. A.; Goyal, Arun. "Computational and SAXS-based structure insights of pectin acetyl esterase (CtPae12B) of family 12 carbohydrate esterase from Clostridium thermocellum ATCC 27405". Journal of Biomolecular Structure and Dynamics (2021): 1-18. http://dx.doi.org/10.1080/07391102.2021.1911858.
    10.1080/07391102.2021.1911858
  7. Alfaia, C.M.; Pestana, J.M.; Rodrigues, M.; Coelho, D.; Aires, M.J.; Ribeiro, D.M.; Major, V.T.; et al. "Influence of dietary Chlorella vulgaris and carbohydrate-active enzymes on growth performance, meat quality and lipid composition of broiler chickens". Poultry Science 100 2 (2021): 926-937. http://dx.doi.org/10.1016/j.psj.2020.11.034.
    10.1016/j.psj.2020.11.034
  8. Duarte, Marlene; Viegas, Aldino; Alves, Victor D.; Prates, José A.M.; Ferreira, Luís M.A.; Najmudin, Shabir; Cabrita, Eurico J.; et al. "A dual cohesin–dockerin complex binding mode in Bacteroides cellulosolvens contributes to the size and complexity of its cellulosome". Journal of Biological Chemistry 296 (2021): 100552. http://dx.doi.org/10.1016/j.jbc.2021.100552.
    10.1016/j.jbc.2021.100552
  9. Coelho, Diogo; Pestana, José; Almeida, João M.; Alfaia, Cristina M.; Fontes, Carlos M. G. A.; Moreira, Olga; Prates, José A. M.. "A High Dietary Incorporation Level of Chlorella vulgaris Improves the Nutritional Value of Pork Fat without Impairing the Performance of Finishing Pigs". Animals 10 12 (2020): 2384. http://dx.doi.org/10.3390/ani10122384.
    10.3390/ani10122384
  10. Reynaud, Steve; Ciolek, Justyna; Degueldre, Michel; Saez, Natalie J.; Sequeira, Ana Filipa; Duhoo, Yoan; Brás, Joana L. A.; et al. "A Venomics Approach Coupled to High-Throughput Toxin Production Strategies Identifies the First Venom-Derived Melanocortin Receptor Agonists". Journal of Medicinal Chemistry 63 15 (2020): 8250-8264. http://dx.doi.org/10.1021/acs.jmedchem.0c00485.
    10.1021/acs.jmedchem.0c00485
  11. Pestana, J.M.; Puerta, B.; Santos, H.; Madeira, M.S.; Alfaia, C.M.; Lopes, P.A.; Pinto, R.M.A.; et al. "Impact of dietary incorporation of Spirulina (Arthrospira platensis) and exogenous enzymes on broiler performance, carcass traits, and meat quality". Poultry Science 99 5 (2020): 2519-2532. http://dx.doi.org/10.1016/j.psj.2019.11.069.
    10.1016/j.psj.2019.11.069
  12. Sharma, Kedar; Fontes, Carlos M. G. A.; Najmudin, Shabir; Goyal, Arun. "Small angle X-ray scattering based structure, modeling and molecular dynamics analyses of family 43 glycoside hydrolase a-L-arabinofuranosidase from Clostridium thermocellum". Journal of Biomolecular Structure and Dynamics 39 1 (2019): 209-218. http://dx.doi.org/10.1080/07391102.2019.1707119.
    10.1080/07391102.2019.1707119
  13. Diana O. Ribeiro; Aldino Viegas; Virgínia M. R. Pires; João Medeiros-Silva; Pedro Bule; Wengang Chai; Filipa Marcelo; et al. "Molecular basis for the preferential recognition of ß1,3-1,4-glucans by the family 11 carbohydrate-binding module from Clostridium thermocellum". The FEBS Journal (2019): https://doi.org/10.1111/febs.15162.
    10.1111/febs.15162
  14. Coelho, D.; Lopes, P.A.; Cardoso, V.; Ponte, P.; Brás, J.; Madeira, M.S.; Alfaia, C.M.; et al. "A two-enzyme constituted mixture to improve the degradation of Arthrospira platensis microalga cell wall for monogastric diets". Journal of Animal Physiology and Animal Nutrition (2019): http://www.scopus.com/inward/record.url?eid=2-s2.0-85074751169&partnerID=MN8TOARS.
    10.1111/jpn.13239
  15. Chong, S.L.; Cardoso, V.; Brás, J.L.A.; Gomes, M.Z.D.V.; Fontes, C.M.G.A.; Olsson, L.. "Immobilization of bacterial feruloyl esterase on mesoporous silica particles and enhancement of synthetic activity by hydrophobic-modified surface". Bioresource Technology 293 (2019): http://www.scopus.com/inward/record.url?eid=2-s2.0-85071631266&partnerID=MN8TOARS.
    10.1016/j.biortech.2019.122009
  16. Sharma, K.; Fontes, C.M.G.A.; Najmudin, S.; Goyal, A.. "Molecular organization and protein stability of the Clostridium thermocellum glucuronoxylan endo-ß-1,4-xylanase of family 30 glycoside hydrolase in solution". Journal of Structural Biology 206 3 (2019): 335-344. http://www.scopus.com/inward/record.url?eid=2-s2.0-85064173596&partnerID=MN8TOARS.
    10.1016/j.jsb.2019.04.005
  17. Cerullo, G.; Varriale, S.; Bozonnet, S.; Antonopoulou, I.; Christakopoulos, P.; Rova, U.; Gherbovet, O.; et al. "Directed evolution of the type C feruloyl esterase from Fusarium oxysporum FoFaeC and molecular docking analysis of its improved variants". New Biotechnology 51 (2019): 14-20. http://www.scopus.com/inward/record.url?eid=2-s2.0-85061183564&partnerID=MN8TOARS.
    10.1016/j.nbt.2019.01.008
  18. Coelho, D.; Lopes, P.A.; Cardoso, V.; Ponte, P.; Brás, J.; Madeira, M.S.; Alfaia, C.M.; et al. "Novel combination of feed enzymes to improve the degradation of Chlorella vulgaris recalcitrant cell wall". Scientific Reports 9 1 (2019): http://www.scopus.com/inward/record.url?eid=2-s2.0-85063731508&partnerID=MN8TOARS.
    10.1038/s41598-019-41775-0
  19. Strazzulli, A.; Cobucci-Ponzano, B.; Iacono, R.; Giglio, R.; Maurelli, L.; Curci, N.; Schiano-di-Cola, C.; et al. "Discovery of hyperstable carbohydrate-active enzymes through metagenomics of extreme environments". FEBS Journal (2019): http://www.scopus.com/inward/record.url?eid=2-s2.0-85074341447&partnerID=MN8TOARS.
    10.1111/febs.15080
  20. Goyal, D.; Kumar, K.; Centeno, M.S.J.; Thakur, A.; Pires, V.M.R.; Bule, P.; Fontes, C.M.G.A.; Goyal, A.. "Molecular Cloning, Expression and Biochemical Characterization of a Family 5 Glycoside Hydrolase First Endo-Mannanase (RfGH5_7) from Ruminococcus flavefaciens FD-1 v3". Molecular Biotechnology 61 11 (2019): 826-835. http://www.scopus.com/inward/record.url?eid=2-s2.0-85071038836&partnerID=MN8TOARS.
    10.1007/s12033-019-00205-2
  21. Andriy Kryshtafovych; Reinhard Albrecht; Arnaud Baslé; Pedro Bule; Alessandro T. Caputo; Ana Luisa Carvalho; Kinlin L. Chao; et al. "Target highlights from the first post-PSI CASP experiment (CASP12, May-August 2016)". Proteins: Structure, Function, and Bioinformatics 86 (2018): 27-50. https://doi.org/10.1002/prot.25392.
    10.1002/prot.25392
  22. Bule, P.; Pires, V.M.R.; Alves, V.D.; Carvalho, A.L.; Prates, J.A.M.; Ferreira, L.M.A.; Smith, S.P.; et al. "Higher order scaffoldin assembly in Ruminococcus flavefaciens cellulosome is coordinated by a discrete cohesin-dockerin interaction". Scientific Reports 8 1 (2018): http://www.scopus.com/inward/record.url?eid=2-s2.0-85046619629&partnerID=MN8TOARS.
    10.1038/s41598-018-25171-8
  23. Cardoso, V.; Fernandes, E.A.; Santos, H.M.M.; Maçãs, B.; Lordelo, M.M.; Telo da Gama, L.; Ferreira, L.M.A.; Fontes, C.M.G.A.; Ribeiro, T.. "Variation in levels of non-starch polysaccharides and endogenous endo-1,4-ß-xylanases affects the nutritive value of wheat for poultry". British Poultry Science 59 2 (2018): 218-226. http://www.scopus.com/inward/record.url?eid=2-s2.0-85043449490&partnerID=MN8TOARS.
    10.1080/00071668.2018.1423674
  24. Bule, P.; Cameron, K.; Prates, J.A.M.; Ferreira, L.M.A.; Smith, S.P.; Gilbert, H.J.; Bayer, E.A.; et al. "Structure-function analyses generate novel specificities to assemble the components of multienzyme bacterial cellulosome complexes". Journal of Biological Chemistry 293 11 (2018): 4201-4212. http://www.scopus.com/inward/record.url?eid=2-s2.0-85044136990&partnerID=MN8TOARS.
    10.1074/jbc.RA117.001241
  25. Kumar, K.; Correia, M.A.S.; Pires, V.M.R.; Dhillon, A.; Sharma, K.; Rajulapati, V.; Fontes, C.M.G.A.; Carvalho, A.L.; Goyal, A.. "Novel insights into the degradation of ß-1,3-glucans by the cellulosome of Clostridium thermocellum revealed by structure and function studies of a family 81 glycoside hydrolase". International Journal of Biological Macromolecules 117 (2018): 890-901. http://www.scopus.com/inward/record.url?eid=2-s2.0-85048200641&partnerID=MN8TOARS.
    10.1016/j.ijbiomac.2018.06.003
  26. Bule, P.; Pires, V.M.; Fontes, C.M.; Alves, V.D.. "Cellulosome assembly: paradigms are meant to be broken!". Current Opinion in Structural Biology 49 (2018): 154-161. http://www.scopus.com/inward/record.url?eid=2-s2.0-85044480888&partnerID=MN8TOARS.
    10.1016/j.sbi.2018.03.012
  27. Varriale, S.; Cerullo, G.; Antonopoulou, I.; Christakopoulos, P.; Rova, U.; Tron, T.; Fauré, R.; et al. "Evolution of the feruloyl esterase MtFae1a from Myceliophthora thermophila towards improved catalysts for antioxidants synthesis". Applied Microbiology and Biotechnology 102 12 (2018): 5185-5196. http://www.scopus.com/inward/record.url?eid=2-s2.0-85045854653&partnerID=MN8TOARS.
    10.1007/s00253-018-8995-4
  28. Ribeiro, T.; Cardoso, V.; Ferreira, L.M.A.; Lordelo, M.M.S.; Coelho, E.; Moreira, A.S.P.; Domingues, M.R.M.; et al. "Xylo-oligosaccharides display a prebiotic activity when used to supplement wheat or corn-based diets for broilers". Poultry science 97 12 (2018): 4330-4341. http://www.scopus.com/inward/record.url?eid=2-s2.0-85059223826&partnerID=MN8TOARS.
    10.3382/ps/pey336
  29. Lee, S.A.; Apajalahti, J.; Vienola, K.; González-Ortiz, G.; Fontes, C.M.G.A.; Bedford, M.R.. "Age and dietary xylanase supplementation affects ileal sugar residues and short chain fatty acid concentration in the ileum and caecum of broiler chickens". Animal Feed Science and Technology 234 (2017): 29-42. http://www.scopus.com/inward/record.url?eid=2-s2.0-85034630412&partnerID=MN8TOARS.
    10.1016/j.anifeedsci.2017.07.017
  30. Israeli-Ruimy, V.; Bule, P.; Jindou, S.; Dassa, B.; Moraïs, S.; Borovok, I.; Barak, Y.; et al. "Complexity of the Ruminococcus flavefaciens FD-1 cellulosome reflects an expansion of family-related protein-protein interactions". Scientific Reports 7 (2017): http://www.scopus.com/inward/record.url?eid=2-s2.0-85012036956&partnerID=MN8TOARS.
    10.1038/srep42355
  31. Bule, P.; Alves, V.D.; Israeli-Ruimy, V.; Carvalho, A.L.; Ferreira, L.M.A.; Smith, S.P.; Gilbert, H.J.; et al. "Assembly of Ruminococcus flavefaciens cellulosome revealed by structures of two cohesin-dockerin complexes". Scientific Reports 7 1 (2017): http://www.scopus.com/inward/record.url?eid=2-s2.0-85018985262&partnerID=MN8TOARS.
    10.1038/s41598-017-00919-w
  32. Sequeira, A.F.; Turchetto, J.; Saez, N.J.; Peysson, F.; Ramond, L.; Duhoo, Y.; Blémont, M.; et al. "Gene design, fusion technology and TEV cleavage conditions influence the purification of oxidized disulphide-rich venom peptides in Escherichia coli". Microbial Cell Factories 16 1 (2017): http://www.scopus.com/inward/record.url?eid=2-s2.0-85009962354&partnerID=MN8TOARS.
    10.1186/s12934-016-0618-0
  33. Turchetto, J.; Sequeira, A.F.; Ramond, L.; Peysson, F.; Brás, J.L.A.; Saez, N.J.; Duhoo, Y.; et al. "High-throughput expression of animal venom toxins in Escherichia coli to generate a large library of oxidized disulphide-reticulated peptides for drug discovery". Microbial Cell Factories 16 1 (2017): http://www.scopus.com/inward/record.url?eid=2-s2.0-85009985911&partnerID=MN8TOARS.
    10.1186/s12934-016-0617-1
  34. Cantante, C.; Lourenço, S.; Morais, M.; Leandro, J.; Gano, L.; Silva, N.; Leandro, P.; et al. "Albumin-binding domain from Streptococcus zooepidemicus protein Zag as a novel strategy to improve the half-life of therapeutic proteins". Journal of Biotechnology 253 (2017): 23-33. http://www.scopus.com/inward/record.url?eid=2-s2.0-85019767367&partnerID=MN8TOARS.
    10.1016/j.jbiotec.2017.05.017
  35. Pires, V.M.R.; Pereira, P.M.M.; Brás, J.L.A.; Correia, M.; Cardoso, V.; Bule, P.; Alves, V.D.; et al. "Stability and ligand promiscuity of type A carbohydrate-binding modules are illustrated by the structure of Spirochaeta thermophila StCBM64C". Journal of Biological Chemistry 292 12 (2017): 4847-4860. http://www.scopus.com/inward/record.url?eid=2-s2.0-85016255875&partnerID=MN8TOARS.
    10.1074/jbc.M116.767541
  36. Venditto, I.; Luis, A.S.; Rydahl, M.; Schückel, J.; Fernandes, V.O.; Vidal-Melgosa, S.; Bule, P.; et al. "Complexity of the Ruminococcus flavefaciens cellulosome reflects an expansion in glycan recognition". Proceedings of the National Academy of Sciences of the United States of America 113 26 (2016): 7136-7141. http://www.scopus.com/inward/record.url?eid=2-s2.0-84976537489&partnerID=MN8TOARS.
    10.1073/pnas.1601558113
  37. Cunha-Santos, C.; Figueira, T.N.; Borrego, P.; Oliveira, S.S.; Rocha, C.; Couto, A.; Cantante, C.; et al. "Development of synthetic light-chain antibodies as novel and potent HIV fusion inhibitors". AIDS 30 11 (2016): 1691-1701. http://www.scopus.com/inward/record.url?eid=2-s2.0-84964048560&partnerID=MN8TOARS.
    10.1097/QAD.0000000000001108
  38. Dhillon, A.; Fernandes, V.O.; Dias, F.M.V.; Prates, J.A.M.; Ferreira, L.M.A.; Fontes, C.M.G.A.; Centeno, M.S.J.; Goyal, A.. "A New Member of Family 11 Polysaccharide Lyase, Rhamnogalacturonan Lyase (CtRGLf) from Clostridium thermocellum". Molecular Biotechnology 58 4 (2016): 232-240. http://www.scopus.com/inward/record.url?eid=2-s2.0-84961404222&partnerID=MN8TOARS.
    10.1007/s12033-016-9921-6
  39. Fernandes, V.O.; Costa, M.; Ribeiro, T.; Serrano, L.; Cardoso, V.; Santos, H.; Lordelo, M.; Ferreira, L.M.A.; Fontes, C.M.G.A.. "1,3-1,4-ß-Glucanases and not 1,4-ß-glucanases improve the nutritive value of barley-based diets for broilers". Animal Feed Science and Technology 211 (2016): 153-163. http://www.scopus.com/inward/record.url?eid=2-s2.0-84955162274&partnerID=MN8TOARS.
    10.1016/j.anifeedsci.2015.11.007
  40. Nash, M.A.; Smith, S.P.; Fontes, C.M.; Bayer, E.A.. "Single versus dual-binding conformations in cellulosomal cohesin–dockerin complexes". Current Opinion in Structural Biology 40 (2016): 89-96. http://www.scopus.com/inward/record.url?eid=2-s2.0-84983515640&partnerID=MN8TOARS.
    10.1016/j.sbi.2016.08.002
  41. Brás, J.L.A.; Pinheiro, B.A.; Cameron, K.; Cuskin, F.; Viegas, A.; Najmudin, S.; Bule, P.; et al. "Diverse specificity of cellulosome attachment to the bacterial cell surface". Scientific Reports 6 (2016): http://www.scopus.com/inward/record.url?eid=2-s2.0-85002651253&partnerID=MN8TOARS.
    10.1038/srep38292
  42. Bule, P.; Alves, V.D.; Leitão, A.; Ferreira, L.M.A.; Bayer, E.A.; Smith, S.P.; Gilbert, H.J.; Najmudin, S.; Fontes, C.M.G.A.. "Single binding mode integration of hemicellulose-degrading enzymes via adaptor scaffoldins in Ruminococcus flavefaciens cellulosome". Journal of Biological Chemistry 291 52 (2016): 26658-26669. http://www.scopus.com/inward/record.url?eid=2-s2.0-85007035674&partnerID=MN8TOARS.
    10.1074/jbc.M116.761643
  43. Sequeira, A.F.; Guerreiro, C.I.P.D.; Vincentelli, R.; Fontes, C.M.G.A.. "T7 Endonuclease I Mediates Error Correction in Artificial Gene Synthesis". Molecular Biotechnology 58 8-9 (2016): 573-584. http://www.scopus.com/inward/record.url?eid=2-s2.0-84975500187&partnerID=MN8TOARS.
    10.1007/s12033-016-9957-7
  44. Goyal, A.; Ahmed, S.; Sharma, K.; Gupta, V.; Bule, P.; Alves, V.D.; Fontes, C.M.G.A.; Najmudin, S.. "Molecular determinants of substrate specificity revealed by the structure of Clostridium thermocellum arabinofuranosidase 43A from glycosyl hydrolase family 43 subfamily 16". Acta Crystallographica Section D: Structural Biology 72 12 (2016): 1281-1289. http://www.scopus.com/inward/record.url?eid=2-s2.0-85005980967&partnerID=MN8TOARS.
    10.1107/S205979831601737X
  45. Mesquita, P.; Garcia, V.; Marques, M.R.; Santos Silva, F.; Oliveira Sousa, M.C.; Carolino, I.; Pimenta, J.; et al. "The prion-related protein (testis-specific) gene (PRNT) is highly polymorphic in Portuguese sheep". Animal Genetics 47 1 (2016): 128-132. http://www.scopus.com/inward/record.url?eid=2-s2.0-84954567894&partnerID=MN8TOARS.
    10.1111/age.12380
  46. Labourel, A.; Crouch, L.I.; Brás, J.L.A.; Jackson, A.; Rogowski, A.; Gray, J.; Yadav, M.P.; et al. "The mechanism by which arabinoxylanases can recognize highly decorated xylans". Journal of Biological Chemistry 291 42 (2016): 22149-22159. http://www.scopus.com/inward/record.url?eid=2-s2.0-84991764831&partnerID=MN8TOARS.
    10.1074/jbc.M116.743948
  47. Freire, F.; Verma, A.; Bule, P.; Alves, V.D.; Fontes, C.M.G.A.; Goyal, A.; Najmudin, S.. "Conservation in the mechanism of glucuronoxylan hydrolysis revealed by the structure of glucuronoxylan xylanohydrolase (CtXyn30A) from Clostridium thermocellum". Acta Crystallographica Section D: Structural Biology 72 11 (2016): 1162-1173. http://www.scopus.com/inward/record.url?eid=2-s2.0-84995953684&partnerID=MN8TOARS.
    10.1107/S2059798316014376
  48. Sequeira, A.F.; Brás, J.L.A.; Guerreiro, C.I.P.D.; Vincentelli, R.; Fontes, C.M.G.A.. "Development of a gene synthesis platform for the efficient large scale production of small genes encoding animal toxins". BMC Biotechnology 16 1 (2016): http://www.scopus.com/inward/record.url?eid=2-s2.0-84999836712&partnerID=MN8TOARS.
    10.1186/s12896-016-0316-3
  49. Cameron, K.; Najmudin, S.; Alves, V.D.; Bayer, E.A.; Smith, S.P.; Bule, P.; Waller, H.; et al. "Cell-surface attachment of bacterial multienzyme complexes involves highly dynamic protein-protein anchors". Journal of Biological Chemistry 290 21 (2015): 13578-13590. http://www.scopus.com/inward/record.url?eid=2-s2.0-84929995654&partnerID=MN8TOARS.
    10.1074/jbc.M114.633339
  50. Venditto, I.; Bule, P.; Thompson, A.; Sanchez-Weatherby, J.; Sandy, J.; Ferreira, L.M.A.; Fontes, C.M.G.A.; Najmudin, S.. "Expression, purification, crystallization and preliminary X-ray analysis of CttA, a putative cellulose-binding protein from Ruminococcus flavefaciens". Acta Crystallographica Section F:Structural Biology Communications 71 (2015): 784-789. http://www.scopus.com/inward/record.url?eid=2-s2.0-84931088457&partnerID=MN8TOARS.
    10.1107/S2053230X15008249
  51. Hernandez-Gomez, M.C.; Rydahl, M.G.; Rogowski, A.; Morland, C.; Cartmell, A.; Crouch, L.; Labourel, A.; et al. "Recognition of xyloglucan by the crystalline cellulose-binding site of a family 3a carbohydrate-binding module". FEBS Letters 589 18 (2015): 2297-2303. http://www.scopus.com/inward/record.url?eid=2-s2.0-84938963685&partnerID=MN8TOARS.
    10.1016/j.febslet.2015.07.009
  52. Cameron, K.; Weinstein, J.Y.; Zhivin, O.; Bule, P.; Fleishman, S.J.; Alves, V.D.; Gilbert, H.J.; et al. "Combined crystal structure of a type I cohesin: Mutation and affinity binding studies reveal structural determinants of Cohesin-dockerin specificities". Journal of Biological Chemistry 290 26 (2015): 16215-16225. http://www.scopus.com/inward/record.url?eid=2-s2.0-84941299732&partnerID=MN8TOARS.
    10.1074/jbc.M115.653303
  53. Palma, A.S.; Liu, Y.; Zhang, H.; Zhang, Y.; McCleary, B.V.; Yu, G.; Huang, Q.; et al. "Unravelling glucan recognition systems by glycome microarrays using the designer approach and mass spectrometry". Molecular and Cellular Proteomics 14 4 (2015): 974-988. http://www.scopus.com/inward/record.url?eid=2-s2.0-84926429839&partnerID=MN8TOARS.
    10.1074/mcp.M115.048272
  54. Chakraborty, S.; Fernandes, V.O.; Dias, F.M.V.; Prates, J.A.M.; Ferreira, L.M.A.; Fontes, C.M.G.A.; Goyal, A.; Centeno, M.S.J.. "Role of pectinolytic enzymes identified in Clostridium thermocellum cellulosome". PLoS ONE 10 2 (2015): http://www.scopus.com/inward/record.url?eid=2-s2.0-84922721372&partnerID=MN8TOARS.
    10.1371/journal.pone.0116787
  55. Venditto, I.; Goyal, A.; Thompson, A.; Ferreira, L.M.A.; Fontes, C.M.G.A.; Najmudin, S.. "Crystallization and preliminary crystallographic studies of a novel noncatalytic carbohydrate-binding module from the Ruminococcus flavefaciens cellulosome". Acta Crystallographica Section F:Structural Biology Communications 71 (2015): 45-48. http://www.scopus.com/inward/record.url?eid=2-s2.0-84921798411&partnerID=MN8TOARS.
    10.1107/S2053230X14025576
  56. Verma, A.K.; Bule, P.; Ribeiro, T.; Brás, J.L.A.; Mukherjee, J.; Gupta, M.N.; Fontes, C.M.G.A.; Goyal, A.. "The family 6 carbohydrate binding module (CtCBM6) of glucuronoxylanase (CtXynGH30) of Clostridium thermocellum binds decorated and undecorated xylans through cleft A". Archives of Biochemistry and Biophysics 575 (2015): 8-21. http://www.scopus.com/inward/record.url?eid=2-s2.0-84927523331&partnerID=MN8TOARS.
    10.1016/j.abb.2015.03.026
  57. Venditto, I.; Najmudin, S.; Luís, A.S.; Ferreira, L.M.A.; Sakka, K.; Knox, J.P.; Gilbert, H.J.; Fontes, C.M.G.A.. "Family 46 carbohydrate-binding modules contribute to the enzymatic hydrolysis of xyloglucan and ß-1,3-1,4-glucans through distinct mechanisms". Journal of Biological Chemistry 290 17 (2015): 10572-10586. http://www.scopus.com/inward/record.url?eid=2-s2.0-84928412955&partnerID=MN8TOARS.
    10.1074/jbc.M115.637827
  58. Pires, A.J.; Ribeiro, T.; Thompson, A.; Venditto, I.; Fernandes, V.O.; Bule, P.; Santos, H.; et al. "Purification and crystallographic studies of a putative carbohydrate-binding module from the Ruminococcus flavefaciens FD-1 endoglucanase Cel5A". Acta Crystallographica Section:F Structural Biology Communications 71 (2015): 958-961. http://www.scopus.com/inward/record.url?eid=2-s2.0-84938853150&partnerID=MN8TOARS.
    10.1107/S2053230X15009784
  59. Costa, M.; Fernandes, V.O.; Ribeiro, T.; Serrano, L.; Cardoso, V.; Santos, H.; Lordelo, M.; Ferreira, L.M.A.; Fontes, C.M.G.A.. "Construction of GH16 ß-glucanase mini-cellulosomes to improve the nutritive value of barley-based diets for broilers". Journal of Agricultural and Food Chemistry 62 30 (2014): 7496-7506. http://www.scopus.com/inward/record.url?eid=2-s2.0-84905270269&partnerID=MN8TOARS.
    10.1021/jf502157y
  60. Das, S.P.; Ravindran, R.; Ghosh, A.; Deka, D.; Das, D.; Jawed, M.; Fontes, C.M.G.A.; Goyal, A.. "Efficient pretreatment for bioethanol production from water hyacinth (Eichhornia crassipes) involving naturally isolated and recombinant enzymes and its recovery". Environmental Progress and Sustainable Energy 33 4 (2014): 1396-1404. http://www.scopus.com/inward/record.url?eid=2-s2.0-84908038390&partnerID=MN8TOARS.
    10.1002/ep.11885
  61. Goyal, A.; Ahmed, S.; Fontes, C.M.G.A.; Najmudin, S.. "Crystallization and preliminary X-ray crystallographic analysis of a novel a-l-arabinofuranosidase (CtGH43) from Clostridium thermocellum ATCC 27405". Acta Crystallographica Section F:Structural Biology Communications 70 5 (2014): 616-618. http://www.scopus.com/inward/record.url?eid=2-s2.0-84905435431&partnerID=MN8TOARS.
    10.1107/S2053230X14006402
  62. Bule, P.; Correia, A.; Cameron, K.; Alves, V.D.; Cardoso, V.; Fontes, C.M.G.A.; Najmudin, S.. "Overexpression, purification, crystallization and preliminary X-ray characterization of the fourth scaffoldin A cohesin from Acetivibrio cellulolyticus in complex with a dockerin from a family 5 glycoside hydrolase". Acta Crystallographica Section F:Structural Biology Communications 70 8 (2014): 1065-1067. http://www.scopus.com/inward/record.url?eid=2-s2.0-84905507495&partnerID=MN8TOARS.
    10.1107/S2053230X14013181
  63. Bule, P.; Ruimy-Israeli, V.; Cardoso, V.; Bayer, E.A.; Fontes, C.M.G.A.; Najmudin, S.. "Overexpression, crystallization and preliminary X-ray characterization of Ruminococcus flavefaciens scaffoldin C cohesin in complex with a dockerin from an uncharacterized CBM-containing protein". Acta Crystallographica Section F:Structural Biology Communications 70 8 (2014): 1061-1064. http://www.scopus.com/inward/record.url?eid=2-s2.0-84905445585&partnerID=MN8TOARS.
    10.1107/S2053230X14012667
  64. Cameron, K.; Alves, V.D.; Bule, P.; Ferreira, L.M.A.; Fontes, C.M.G.A.; Najmudin, S.. "Purification, crystallization and preliminary X-ray characterization of the third ScaB cohesin in complex with an ScaA X-dockerin from Acetivibrio cellulolyticus". Acta Crystallographica Section F:Structural Biology Communications 70 5 (2014): 656-658. http://www.scopus.com/inward/record.url?eid=2-s2.0-84905502987&partnerID=MN8TOARS.
    10.1107/S2053230X1400750X
  65. Venditto, I.; Santos, H.; Ferreira, L.M.A.; Sakka, K.; Fontes, C.M.G.A.; Najmudin, S.. "Overproduction, purification, crystallization and preliminary X-ray characterization of the family 46 carbohydrate-binding module (CBM46) of endo-ß-1,4-glucanase B (CelB) from Bacillus halodurans". Acta Crystallographica Section F:Structural Biology Communications 70 6 (2014): 754-757. http://www.scopus.com/inward/record.url?eid=2-s2.0-84905494649&partnerID=MN8TOARS.
    10.1107/S2053230X14008395
  66. Cardoso, V.; Ferreira, A.P.; Costa, M.; Ponte, P.I.P.; Falcão, L.; Freire, J.P.; Lordelo, M.M.; et al. "Temporal restriction of enzyme supplementation in barley-based diets has no effect in broiler performance". Animal Feed Science and Technology 198 (2014): 186-195. http://www.scopus.com/inward/record.url?eid=2-s2.0-84918796052&partnerID=MN8TOARS.
    10.1016/j.anifeedsci.2014.09.007
  67. Venditto, I.; Santos, H.; Sandy, J.; Sanchez-Weatherby, J.; Ferreira, L.M.A.; Sakka, K.; Fontes, C.M.G.A.; Najmudin, S.. "Crystallization and preliminary X-ray diffraction analysis of a trimodular endo-ß-1,4-glucanase (Cel5B) from Bacillus halodurans". Acta Crystallographica Section F:Structural Biology Communications 70 (2014): 1628-1630. http://www.scopus.com/inward/record.url?eid=2-s2.0-84925884238&partnerID=MN8TOARS.
    10.1107/S2053230X1402319X
  68. Ghosh, A.; Verma, A.K.; Luis, A.S.; Bras, J.L.A.; Fontes, C.M.; Goyal, A.. "Mannan specific family 35 carbohydrate-binding module (CtCBM35) of Clostridium thermocellum: structure analysis and ligand binding". Biologia (Poland) 69 10 (2014): 1271-1282. http://www.scopus.com/inward/record.url?eid=2-s2.0-84920263154&partnerID=MN8TOARS.
    10.2478/s11756-014-0444-y
  69. da Costa, A.S.H.; Bessa, R.J.B.; Pires, V.M.R.; Rolo, E.A.; Pinto, R.M.A.; Andrade Fontes, C.M.G.; Prates, J.A.M.. "Is hepatic lipid metabolism of beef cattle influenced by breed and dietary silage level?". BMC Veterinary Research 10 (2014): http://www.scopus.com/inward/record.url?eid=2-s2.0-84899140808&partnerID=MN8TOARS.
    10.1186/1746-6148-10-65
  70. Ribeiro, T.; Lordelo, M.M.; Costa, P.; Alves, S.P.; Benevides, W.S.; Bessa, R.J.B.; Lemos, J.P.C.; et al. "Effect of reduced dietary protein and supplementation with a docosahexaenoic acid product on broiler performance and meat quality". British Poultry Science 55 6 (2014): 752-765. http://www.scopus.com/inward/record.url?eid=2-s2.0-84919777689&partnerID=MN8TOARS.
    10.1080/00071668.2014.971222
  71. Morais, M.; Cantante, C.; Gano, L.; Santos, I.; Lourenço, S.; Santos, C.; Fontes, C.; et al. "Biodistribution of a 67Ga-labeled anti-TNF VHH single-domain antibody containing a bacterial albumin-binding domain (Zag)". Nuclear Medicine and Biology 41 S (2014): http://www.scopus.com/inward/record.url?eid=2-s2.0-84899084462&partnerID=MN8TOARS.
    10.1016/j.nucmedbio.2014.01.009
  72. Hamberg, Y.; Ruimy-Israeli, V.; Dassa, B.; Barak, Y.; Lamed, R.; Cameron, K.; Fontes, C.M.G.A.; Bayer, E.A.; Fried, D.B.. "Elaborate cellulosome architecture of Acetivibrio cellulolyticus revealed by selective screening of cohesin-dockerin interactions". PeerJ 2014 1 (2014): http://www.scopus.com/inward/record.url?eid=2-s2.0-84910034782&partnerID=MN8TOARS.
    10.7717/peerj.636
  73. Venditto, I.; Centeno, M.S.J.; Ferreira, L.M.A.; Fontes, C.M.G.A.; Najmudin, S.. "Expression, purification and crystallization of a novel carbohydrate-binding module from the Ruminococcus flavefaciens cellulosome". Acta Crystallographica Section F:Structural Biology Communications 70 (2014): 1653-1656. http://www.scopus.com/inward/record.url?eid=2-s2.0-84925884710&partnerID=MN8TOARS.
    10.1107/S2053230X14024248
  74. Ghosh, A.; Luís, A.S.; Brás, J.L.A.; Pathaw, N.; Chrungoo, N.K.; Fontes, C.M.G.A.; Goyal, A.. "Deciphering ligand specificity of a Clostridium thermocellum family 35 carbohydrate binding module (CtCBM35) for gluco- and galacto- Substituted mannans and its calcium induced stability". PLoS ONE 8 12 (2013): http://www.scopus.com/inward/record.url?eid=2-s2.0-84891934604&partnerID=MN8TOARS.
    10.1371/journal.pone.0080415
  75. Costa, A.S.H.; Costa, P.; Bessa, R.J.B.; Lemos, J.P.C.; Simões, J.A.; Santos-Silva, J.; Fontes, C.M.G.A.; Prates, J.A.M.. "Carcass fat partitioning and meat quality of Alentejana and Barrosã young bulls fed high or low maize silage diets". Meat Science 93 3 (2013): 405-412. http://www.scopus.com/inward/record.url?eid=2-s2.0-84870201492&partnerID=MN8TOARS.
    10.1016/j.meatsci.2012.10.010
  76. da Costa, A.S.H.; Pires, V.M.R.; Fontes, C.M.G.A.; Mestre Prates, J.A.. "Expression of genes controlling fat deposition in two genetically diverse beef cattle breeds fed high or low silage diets". BMC Veterinary Research 9 (2013): http://www.scopus.com/inward/record.url?eid=2-s2.0-84879053446&partnerID=MN8TOARS.
    10.1186/1746-6148-9-118
  77. Ribeiro, T.; Lordelo, M.M.; Alves, S.P.; Bessa, R.J.B.; Costa, P.; Lemos, J.P.C.; Ferreira, L.M.A.; Fontes, C.M.G.A.; Prates, J.A.M.. "Direct supplementation of diet is the most efficient way of enriching broiler meat with n-3 long-chain polyunsaturated fatty acids". British Poultry Science 54 6 (2013): 753-765. http://www.scopus.com/inward/record.url?eid=2-s2.0-84892467912&partnerID=MN8TOARS.
    10.1080/00071668.2013.841861
  78. Ahmed, S.; Luis, A.S.; Bras, J.L.A.; Ghosh, A.; Gautam, S.; Gupta, M.N.; Fontes, C.M.G.A.; Goyal, A.. "A Novel a-L-Arabinofuranosidase of Family 43 Glycoside Hydrolase (Ct43Araf) from Clostridium thermocellum". PLoS ONE 8 9 (2013): http://www.scopus.com/inward/record.url?eid=2-s2.0-84883641015&partnerID=MN8TOARS.
    10.1371/journal.pone.0073575
  79. Verma, A.K.; Goyal, A.; Freire, F.; Bule, P.; Venditto, I.; Brás, J.L.A.; Santos, H.; et al. "Overexpression, crystallization and preliminary X-ray crystallographic analysis of glucuronoxylan xylanohydrolase (Xyn30A) from Clostridium thermocellum". Acta Crystallographica Section F: Structural Biology and Crystallization Communications 69 12 (2013): 1440-1442. http://www.scopus.com/inward/record.url?eid=2-s2.0-84890011176&partnerID=MN8TOARS.
    10.1107/S1744309113025050
  80. Pimenta, J.; Viegas, A.; Sardinha, J.; Martins, I.C.; Cabrita, E.J.; Fontes, C.M.G.A.; Prates, J.A.; Pereira, R.M.L.N.. "NMR solution structure and SRP54M predicted interaction of the N-terminal sequence (1-30) of the ovine Doppel protein". Peptides 49 (2013): 32-40. http://www.scopus.com/inward/record.url?eid=2-s2.0-84884176200&partnerID=MN8TOARS.
    10.1016/j.peptides.2013.08.013
  81. Currie, M.A.; Cameron, K.; Dias, F.M.V.; Spencer, H.L.; Bayer, E.A.; Fontes, C.M.G.A.; Smith, S.P.; Jia, Z.. "Small angle x-ray scattering analysis of clostridium thermocellum cellulosome N-terminal complexes reveals a highly dynamic structure". Journal of Biological Chemistry 288 11 (2013): 7978-7985. http://www.scopus.com/inward/record.url?eid=2-s2.0-84875189918&partnerID=MN8TOARS.
    10.1074/jbc.M112.408757
  82. Venditto, I.; Baslé, A.; Luís, A.S.; Temple, M.J.; Ferreira, L.M.A.; Fontes, C.M.G.A.; Gilbert, H.J.; Najmudin, S.. "Overproduction, purification, crystallization and preliminary X-ray characterization of the C-terminal family 65 carbohydrate-binding module (CBM65B) of endoglucanase Cel5A from Eubacterium cellulosolvens". Acta Crystallographica Section F: Structural Biology and Crystallization Communications 69 2 (2013): 191-194. http://www.scopus.com/inward/record.url?eid=2-s2.0-84873598839&partnerID=MN8TOARS.
    10.1107/S1744309113001620
  83. Costa, A.S.H.; Silva, M.P.; Alfaia, C.P.M.; Pires, V.M.R.; Fontes, C.M.G.A.; Bessa, R.J.B.; Prates, J.A.M.. "Genetic background and diet impact beef fatty acid composition and stearoyl-CoA desaturase mRNA expression". Lipids 48 4 (2013): 369-381. http://www.scopus.com/inward/record.url?eid=2-s2.0-84876290027&partnerID=MN8TOARS.
    10.1007/s11745-013-3776-4
  84. Ahmed, S.; Luís, A.S.; Brás, J.L.A.; Fontes, C.M.G.A.; Goyal, A.. "Functional and structural characterization of family 6 carbohydrate-binding module (CtCBM6A) of Clostridium thermocellum a-L-arabinofuranosidase". Biochemistry (Moscow) 78 11 (2013): 1272-1279. http://www.scopus.com/inward/record.url?eid=2-s2.0-84889015905&partnerID=MN8TOARS.
    10.1134/S0006297913110072
  85. Mendes, A.R.; Ribeiro, T.; Correia, B.A.; Bule, P.; Maçãs, B.; Falcão, L.; Freire, J.P.B.; et al. "Low doses of exogenous xylanase improve the nutritive value of triticale-based diets for broilers". Journal of Applied Poultry Research 22 1 (2013): 92-99. http://www.scopus.com/inward/record.url?eid=2-s2.0-84874407651&partnerID=MN8TOARS.
    10.3382/japr.2012-00610
  86. Pimenta, J.; Sardinha, J.; Marques, C.C.; Domingos, A.; Baptista, M.C.; Barbas, J.P.; Martins, I.C.; et al. "Inhibition of ovine in vitro fertilization by anti-Prt antibody: Hypothetical model for Prt/ZP interaction". Reproductive Biology and Endocrinology 11 1 (2013): http://www.scopus.com/inward/record.url?eid=2-s2.0-84875307776&partnerID=MN8TOARS.
    10.1186/1477-7827-11-25
  87. Luís, A.S.; Venditto, I.; Temple, M.J.; Rogowski, A.; Baslé, A.; Xue, J.; Knox, J.P.; et al. "Understanding how noncatalytic carbohydrate binding modules can display specificity for xyloglucan". Journal of Biological Chemistry 288 7 (2013): 4799-4809. http://www.scopus.com/inward/record.url?eid=2-s2.0-84873599781&partnerID=MN8TOARS.
    10.1074/jbc.M112.432781
  88. Santos, C.I.; Ribeiro, T.; Ponte, P.I.P.; Fernandes, V.O.; Falcão, L.; Freire, J.P.; Prates, J.A.M.; et al. "The effects of restricting enzyme supplementation in rye-based diets for broilers". Animal Feed Science and Technology 186 3-4 (2013): 214-217. http://www.scopus.com/inward/record.url?eid=2-s2.0-84888827872&partnerID=MN8TOARS.
    10.1016/j.anifeedsci.2013.10.011
  89. Viegas, A.; Sardinha, J.; Freire, F.; Duarte, D.F.; Carvalho, A.L.; Fontes, C.M.G.A.; Romão, M.J.; Macedo, A.L.; Cabrita, E.J.. "Solution structure, dynamics and binding studies of a family 11 carbohydrate-binding module from clostridium thermocellum (Ct CBM11)". Biochemical Journal 451 2 (2013): 289-300. http://www.scopus.com/inward/record.url?eid=2-s2.0-84875612824&partnerID=MN8TOARS.
    10.1042/BJ20120627
  90. Ghosh, A.; Luís, A.S.; Brás, J.L.A.; Fontes, C.M.G.A.; Goyal, A.. "Thermostable Recombinant ß-(1¿4)-Mannanase from C. thermocellum: Biochemical characterization and manno-oligosaccharides production". Journal of Agricultural and Food Chemistry 61 50 (2013): 12333-12344. http://www.scopus.com/inward/record.url?eid=2-s2.0-84890679086&partnerID=MN8TOARS.
    10.1021/jf403111g
  91. Ahmed, S.; Luis, A.S.; Brás, J.L.A.; Fontes, C.M.G.A.; Goyal, A.. "The family 6 carbohydrate-binding module (CtCBM6B) of Clostridium thermocellum alpha-L-arabinofuranosidase binds xylans and thermally stabilized by Ca2+ ions". Biocatalysis and Biotransformation 31 4 (2013): 217-225. http://www.scopus.com/inward/record.url?eid=2-s2.0-84882590290&partnerID=MN8TOARS.
    10.3109/10242422.2013.828047
  92. Das, S.P.; Ravindran, R.; Ahmed, S.; Das, D.; Goyal, D.; Fontes, C.M.G.A.; Goyal, A.. "Bioethanol production involving recombinant C. thermocellum hydrolytic hemicellulase and fermentative microbes". Applied Biochemistry and Biotechnology 167 6 (2012): 1475-1488. http://www.scopus.com/inward/record.url?eid=2-s2.0-84866354199&partnerID=MN8TOARS.
    10.1007/s12010-012-9618-7
  93. Pimenta, J.; Domingos, A.; Santos, P.; Marques, C.C.; Cantante, C.; Santos, A.; Barbas, J.P.; et al. "Is prnt a pseudogene? identification of ram prt in testis and ejaculated spermatozoa". PLoS ONE 7 8 (2012): http://www.scopus.com/inward/record.url?eid=2-s2.0-84865296947&partnerID=MN8TOARS.
    10.1371/journal.pone.0042957
  94. Mizutani, K.; Fernandes, V.O.; Karita, S.; Luís, A.S.; Sakka, M.; Kimura, T.; Jackson, A.; et al. "Influence of a mannan binding family 32 carbohydrate binding module on the activity of the appended mannanase". Applied and Environmental Microbiology 78 14 (2012): 4781-4787. http://www.scopus.com/inward/record.url?eid=2-s2.0-84863770682&partnerID=MN8TOARS.
    10.1128/AEM.07457-11
  95. Cameron, K.; Alves, V.D.; Bule, P.; Ferreira, L.M.A.; Fontes, C.M.G.A.; Najmudin, S.. "Purification, crystallization and preliminary X-ray characterization of the Acetivibrio cellulolyticus type i cohesin ScaC in complex with the ScaB dockerin". Acta Crystallographica Section F: Structural Biology and Crystallization Communications 68 9 (2012): 1030-1033. http://www.scopus.com/inward/record.url?eid=2-s2.0-84865727287&partnerID=MN8TOARS.
    10.1107/S1744309112031922
  96. Brás, J.L.A.; Alves, V.D.; Carvalho, A.L.; Najmudin, S.; Prates, J.A.M.; Ferreira, L.M.A.; Bolam, D.N.; et al. "Novel Clostridium thermocellum type I cohesin-dockerin complexes reveal a single binding mode". Journal of Biological Chemistry 287 53 (2012): 44394-44405. http://www.scopus.com/inward/record.url?eid=2-s2.0-84871786607&partnerID=MN8TOARS.
    10.1074/jbc.M112.407700
  97. Fontes, C.M.G.A.; Correia, I.S.; Romão, M.J.; Sá-Nogueira, I.; Prates, J.A.M.; Ferreira, L.M.A.. "Editorial". Biocatalysis and Biotransformation 30 3 (2012): http://www.scopus.com/inward/record.url?eid=2-s2.0-84861752070&partnerID=MN8TOARS.
    10.3109/10242422.2012.681858
  98. Costa, A.S.H.; Lopes, P.A.; Estevão, M.; Martins, S.V.; Alves, S.P.; Pinto, R.M.A.; Pissarra, H.; et al. "Contrasting cellularity and fatty acid composition in fat depots from Alentejana and Barrosã bovine breeds fed high and low forage diets". International Journal of Biological Sciences 8 2 (2012): 214-227. http://www.scopus.com/inward/record.url?eid=2-s2.0-84856165348&partnerID=MN8TOARS.
  99. Pinheiro, B.A.; Brás, J.L.A.; Najmudin, S.; Carvalho, A.L.; Ferreira, L.M.A.; Prates, J.A.M.; Fontes, C.M.G.A.. "Flexibility and specificity of the cohesindockerin interaction: Implications for cellulosome assembly and functionality". Biocatalysis and Biotransformation 30 3 (2012): 309-315. http://www.scopus.com/inward/record.url?eid=2-s2.0-84861742005&partnerID=MN8TOARS.
    10.3109/10242422.2012.681854
  100. Pimenta, J.; Dias, F.M.V.; Marques, C.C.; Baptista, M.C.; Vasques, M.I.; Horta, A.E.M.; Barbas, J.P.; et al. "The Prion-like Protein Doppel Enhances Ovine Spermatozoa Fertilizing Ability". Reproduction in Domestic Animals 47 2 (2012): 196-202. http://www.scopus.com/inward/record.url?eid=2-s2.0-84858438080&partnerID=MN8TOARS.
    10.1111/j.1439-0531.2011.01827.x
  101. Figueiredo, A.A.; Correia, B.A.; Ribeiro, T.; Ponte, P.I.P.; Falcão, L.; Freire, J.P.; Prates, J.A.M.; et al. "The effects of restricting enzyme supplementation in wheat-based diets to broilers". Animal Feed Science and Technology 172 3-4 (2012): 194-200. http://www.scopus.com/inward/record.url?eid=2-s2.0-84857448031&partnerID=MN8TOARS.
    10.1016/j.anifeedsci.2012.01.001
  102. Ribeiro, T.; Lordelo, M.M.S.; Prates, J.A.M.; Falcão, L.; Freire, J.P.B.; Ferreira, L.M.A.; Fontes, C.M.G.A.. "The thermostable ß-1,3-1,4-glucanase from Clostridium thermocellum improves the nutritive value of highly viscous barley-based diets for broilers". British Poultry Science 53 2 (2012): 224-234. http://www.scopus.com/inward/record.url?eid=2-s2.0-84861742676&partnerID=MN8TOARS.
    10.1080/00071668.2012.674632
  103. Ribeiro, T.; Lordelo, M.M.S.; Ponte, P.I.P.; Maçãs, B.; Prates, J.A.M.; Aguiar Fontes, M.; Falcão, L.; et al. "Levels of endogenous ß-glucanase activity in barley affect the efficacy of exogenous enzymes used to supplement barley-based diets for poultry". Poultry Science 90 6 (2011): 1245-1256. http://www.scopus.com/inward/record.url?eid=2-s2.0-79957489929&partnerID=MN8TOARS.
    10.3382/ps.2010-01218
  104. Montanier, C.Y.; Correia, M.A.S.; Flint, J.E.; Zhu, Y.; Baslé, A.; McKee, L.S.; Prates, J.A.M.; et al. "A novel, noncatalytic carbohydrate-binding module displays specificity for galactose-containing polysaccharides through calcium-mediated oligomerization". Journal of Biological Chemistry 286 25 (2011): 22499-22509. http://www.scopus.com/inward/record.url?eid=2-s2.0-79959362561&partnerID=MN8TOARS.
    10.1074/jbc.M110.217372
  105. Brás, J.L.A.; Cartmell, A.; Carvalho, A.L.M.; Verzé, G.; Bayer, E.A.; Vazana, Y.; Correia, M.A.S.; et al. "Structural insights into a unique cellulase fold and mechanism of cellulose hydrolysis (Proceedings of the National Academy of Sciences of the United States of America (2011) 108, 13, (5237-5242))". Proceedings of the National Academy of Sciences of the United States of America 108 20 (2011): http://www.scopus.com/inward/record.url?eid=2-s2.0-79957764741&partnerID=MN8TOARS.
    10.1073/pnas.1104766108
  106. Brás, J.L.A.; Cartmell, A.; Carvalho, A.L.M.; Verzé, G.; Bayer, E.A.; Vazana, Y.; Correia, M.A.S.; et al. "Structural insights into a unique cellulase fold and mechanism of cellulose hydrolysis". Proceedings of the National Academy of Sciences of the United States of America 108 13 (2011): 5237-5242. http://www.scopus.com/inward/record.url?eid=2-s2.0-79955116598&partnerID=MN8TOARS.
    10.1073/pnas.1015006108
  107. Correia, M.A.S.; Mazumder, K.; Brás, J.L.A.; Firbank, S.J.; Zhu, Y.; Lewis, R.J.; York, W.S.; Fontes, C.M.G.A.; Gilbert, H.J.. "Structure and function of an arabinoxylan-specific xylanase". Journal of Biological Chemistry 286 25 (2011): 22510-22520. http://www.scopus.com/inward/record.url?eid=2-s2.0-79959344380&partnerID=MN8TOARS.
    10.1074/jbc.M110.217315
  108. Luís, A.S.; Alves, V.D.; Romão, M.J.; Prates, J.A.M.; Fontes, C.M.G.A.; Najmudin, S.. "Overproduction, purification, crystallization and preliminary X-ray characterization of a novel carbohydrate-binding module of endoglucanase Cel5A from Eubacterium cellulosolvens". Acta Crystallographica Section F: Structural Biology and Crystallization Communications 67 4 (2011): 491-493. http://www.scopus.com/inward/record.url?eid=2-s2.0-79955111233&partnerID=MN8TOARS.
    10.1107/S1744309111004246
  109. García-Alvarez, B.; Melero, R.; Dias, F.M.V.; Prates, J.A.M.; Fontes, C.M.G.A.; Smith, S.P.; Romão, M.J.; Carvalho, A.L.; Llorca, O.. "Molecular architecture and structural transitions of a clostridium thermocellum mini-cellulosome". Journal of Molecular Biology 407 4 (2011): 571-580. http://www.scopus.com/inward/record.url?eid=2-s2.0-79952449019&partnerID=MN8TOARS.
    10.1016/j.jmb.2011.01.060
  110. Brás, J.L.A.; Correia, M.A.S.; Romão, M.J.; Prates, J.A.M.; Fontes, C.M.G.A.; Najmudin, S.. "Purification, crystallization and preliminary X-ray characterization of the pentamodular arabinoxylanase CtXyl5A from Clostridium thermocellum". Acta Crystallographica Section F: Structural Biology and Crystallization Communications 67 7 (2011): 833-836. http://www.scopus.com/inward/record.url?eid=2-s2.0-79960949534&partnerID=MN8TOARS.
    10.1107/S1744309111020823
  111. Sossidou, E.N.; Dal Bosco, A.; Elson, H.A.; Fontes, C.M.G.A.. "Pasture-based systems for poultry production: Implications and perspectives". World's Poultry Science Journal 67 1 (2011): 47-58. http://www.scopus.com/inward/record.url?eid=2-s2.0-80054026178&partnerID=MN8TOARS.
    10.1017/S0043933911000043
  112. Correia, M.A.S.; Abbott, D.W.; Gloster, T.M.; Fernandes, V.O.; Prates, J.A.M.; Montanier, C.; Dumon, C.; et al. "Signature active site architectures illuminate the molecular basis for ligand specificity in family 35 carbohydrate binding module". Biochemistry 49 29 (2010): 6193-6205. http://www.scopus.com/inward/record.url?eid=2-s2.0-77954820111&partnerID=MN8TOARS.
    10.1021/bi1006139
  113. Najmudin, S.; Pinheiro, B.A.; Prates, J.A.M.; Gilbert, H.J.; Romão, M.J.; Fontes, C.M.G.A.. "Putting an N-terminal end to the Clostridium thermocellum xylanase Xyn10B story: Crystal structure of the CBM22-1-GH10 modules complexed with xylohexaose". Journal of Structural Biology 172 3 (2010): 353-362. http://www.scopus.com/inward/record.url?eid=2-s2.0-77957964845&partnerID=MN8TOARS.
    10.1016/j.jsb.2010.07.009
  114. Alfaia, C.M.M.; Alves, S.P.; Lopes, A.F.; Fernandes, M.J.E.; Costa, A.S.H.; Fontes, C.M.G.A.; Castro, M.L.F.; Bessa, R.J.B.; Prates, J.A.M.. "Effect of cooking methods on fatty acids, conjugated isomers of linoleic acid and nutritional quality of beef intramuscular fat". Meat Science 84 4 (2010): 769-777. http://www.scopus.com/inward/record.url?eid=2-s2.0-76649126726&partnerID=MN8TOARS.
    10.1016/j.meatsci.2009.11.014
  115. Mesquita, P.; Batista, M.; Marques, M.R.; Santos, I.C.; Pimenta, J.; Silva Pereira, M.; Carolino, I.; et al. "Prion-like Doppel gene polymorphisms and scrapie susceptibility in portuguese sheep breeds". Animal Genetics 41 3 (2010): 311-314. http://www.scopus.com/inward/record.url?eid=2-s2.0-77952054169&partnerID=MN8TOARS.
    10.1111/j.1365-2052.2009.01992.x
  116. Ribeiro, T.; Santos-Silva, T.; Alves, V.D.; Dias, F.M.V.; Luís, A.S.; Prates, J.A.M.; Ferreira, L.M.A.; Romão, M.J.; Fontes, C.M.G.A.. "Family 42 carbohydrate-binding modules display multiple arabinoxylan-binding interfaces presenting different ligand affinities". Biochimica et Biophysica Acta - Proteins and Proteomics 1804 10 (2010): 2054-2062. http://www.scopus.com/inward/record.url?eid=2-s2.0-77956061008&partnerID=MN8TOARS.
    10.1016/j.bbapap.2010.07.006
  117. Ahmed, S.; Deka, D.; Jawed, M.; Goyal, D.; Fontes, C.M.G.A.; Goyal, A.. "Biochemical characterization of a recombinant derivative (CtLic26A-Cel5) of a cellulosomal cellulase from Clostridium thermocellum". Current Trends in Biotechnology and Pharmacy 3 1 (2009): 56-63. http://www.scopus.com/inward/record.url?eid=2-s2.0-62849122817&partnerID=MN8TOARS.
  118. Ahmed, S.; Bharali, S.; Purama, R.K.; Majumder, A.; Fontes, C.M.G.A.; Goyal, A.. "Structural and biochemical properties of lichenase from Clostridium thermocellum". Indian Journal of Microbiology 49 1 (2009): 72-76. http://www.scopus.com/inward/record.url?eid=2-s2.0-69549113120&partnerID=MN8TOARS.
    10.1007/s12088-009-0003-3
  119. Pinheiro, B.A.; Gilbert, H.J.; Sakka, K.; Fernandes, V.O.; Prates, J.A.M.; Alves, V.D.; Bolam, D.N.; Ferreira, L.M.A.; Fontes, C.M.G.A.. "Functional insights into the role of novel type I cohesin and dockerin domains from Clostridium thermocellum". Biochemical Journal 424 3 (2009): 375-384. http://www.scopus.com/inward/record.url?eid=2-s2.0-73149122122&partnerID=MN8TOARS.
    10.1042/BJ20091152
  120. Montanier, C.; Money, V.A.; Pires, V.M.R.; Flint, J.E.; Pinheiro, B.A.; Goyal, A.; Prates, J.A.M.; et al. "The active site of a carbohydrate esterase displays divergent catalytic and noncatalytic binding functions". PLoS Biology 7 3 (2009): 0687-0697. http://www.scopus.com/inward/record.url?eid=2-s2.0-66149093843&partnerID=MN8TOARS.
  121. Alfaia, C.P.M.; Alves, S.P.; Martins, S.I.V.; Costa, A.S.H.; Fontes, C.M.G.A.; Lemos, J.P.C.; Bessa, R.J.B.; Prates, J.A.M.. "Effect of the feeding system on intramuscular fatty acids and conjugated linoleic acid isomers of beef cattle, with emphasis on their nutritional value and discriminatory ability". Food Chemistry 114 3 (2009): 939-946. http://www.scopus.com/inward/record.url?eid=2-s2.0-59849084330&partnerID=MN8TOARS.
    10.1016/j.foodchem.2008.10.041
  122. Pereira, R.M.; Mesquita, P.; Batista, M.; Baptista, M.C.; Barbas, J.P.; Pimenta, J.; Santos, I.C.; et al. "Doppel gene polymorphisms in Portuguese sheep breeds: Insights on ram fertility". Animal Reproduction Science 114 1-3 (2009): 157-166. http://www.scopus.com/inward/record.url?eid=2-s2.0-67349119029&partnerID=MN8TOARS.
    10.1016/j.anireprosci.2008.10.003
  123. Correia, M.A.S.; Pires, V.M.R.; Gilbert, H.J.; Bolam, D.N.; Fernandes, V.O.; Alves, V.D.; Prates, J.A.M.; Ferreira, L.M.A.; Fontes, C.M.G.A.. "Family 6 carbohydrate-binding modules display multiple ß1,3-linked glucan-specific binding interfaces". FEMS Microbiology Letters 300 1 (2009): 48-57. http://www.scopus.com/inward/record.url?eid=2-s2.0-70349667081&partnerID=MN8TOARS.
    10.1111/j.1574-6968.2009.01764.x
  124. Montanier, C.; Van Bueren, A.L.; Dumon, C.; Flint, J.E.; Correia, M.A.; Prates, J.A.; Firbank, S.J.; et al. "Evidence that family 35 carbohydrate binding modules display conserved specificity but divergent function". Proceedings of the National Academy of Sciences of the United States of America 106 9 (2009): 3065-3070. http://www.scopus.com/inward/record.url?eid=2-s2.0-62549151714&partnerID=MN8TOARS.
    10.1073/pnas.0808972106
  125. Pante, P.I.P.; Prates, J.A.M.; Crespo, J.P.; Crespo, D.G.; Mourão, J.L.; Alves, S.P.; Bessa, R.J.B.; et al. "Improving the lipid nutritive value of poultry meat through the incorporation of a dehydrated leguminous-based forage in the diet for broiler chicks". Poultry Science 87 8 (2008): 1587-1594. http://www.scopus.com/inward/record.url?eid=2-s2.0-49449108401&partnerID=MN8TOARS.
    10.3382/ps.2007-00446
  126. Guerreiro, C.I.P.D.; Fontes, C.M.G.A.; Gama, M.; Domingues, L.. "Escherichia coli expression and purification of four antimicrobial peptides fused to a family 3 carbohydrate-binding module (CBM) from Clostridium thermocellum". Protein Expression and Purification 59 1 (2008): 161-168. http://www.scopus.com/inward/record.url?eid=2-s2.0-40949085125&partnerID=MN8TOARS.
    10.1016/j.pep.2008.01.018
  127. Viegas, A.; Brás, N.F.; Cerqueira, N.M.F.S.A.; Fernandes, P.A.; Prates, J.A.M.; Fontes, C.M.G.A.; Bruix, M.; et al. "Molecular determinants of ligand specificity in family 11 carbohydrate binding modules - An NMR, X-ray crystallography and computational chemistry approach". FEBS Journal 275 10 (2008): 2524-2535. http://www.scopus.com/inward/record.url?eid=2-s2.0-42649116746&partnerID=MN8TOARS.
    10.1111/j.1742-4658.2008.06401.x
  128. Ponte, P.I.P.; Alves, S.P.; Bessa, R.J.B.; Ferreira, L.M.A.; Gama, L.T.; Brás, J.L.A.; Fontes, C.M.G.A.; Prates, J.A.M.. "Influence of pasture intake on the fatty acid composition, and cholesterol, tocopherols, and tocotrienols content in meat from free-range broilers". Poultry Science 87 1 (2008): 80-88. http://www.scopus.com/inward/record.url?eid=2-s2.0-37849027999&partnerID=MN8TOARS.
    10.3382/ps.2007-00148
  129. Correia, M.A.S.; Prates, J.A.M.; Brás, J.; Fontes, C.M.G.A.; Newman, J.A.; Lewis, R.J.; Gilbert, H.J.; Flint, J.E.. "Crystal Structure of a Cellulosomal Family 3 Carbohydrate Esterase from Clostridium thermocellum Provides Insights into the Mechanism of Substrate Recognition". Journal of Molecular Biology 379 1 (2008): 64-72. http://www.scopus.com/inward/record.url?eid=2-s2.0-43049170350&partnerID=MN8TOARS.
    10.1016/j.jmb.2008.03.037
  130. Ponte, P.I.P.; Lordelo, M.M.S.; Guerreiro, C.I.P.D.; Soares, M.C.; Mourão, J.L.; Crespo, J.P.; Crespo, D.G.; et al. "Crop ß-glucanase activity limits the effectiveness of a recombinant cellulase used to supplement a barley-based feed for free-range broilers". British Poultry Science 49 3 (2008): 347-359. http://www.scopus.com/inward/record.url?eid=2-s2.0-45849136370&partnerID=MN8TOARS.
    10.1080/00071660802158340
  131. Mourão, J.L.; Pinheiro, V.M.; Prates, J.A.M.; Bessa, R.J.B.; Ferreira, L.M.A.; Fontes, C.M.G.A.; Ponte, P.I.P.. "Effect of dietary dehydrated pasture and citrus pulp on the performance and meat quality of broiler chickens". Poultry Science 87 4 (2008): 733-743. http://www.scopus.com/inward/record.url?eid=2-s2.0-42649089471&partnerID=MN8TOARS.
    10.3382/ps.2007-00411
  132. Lopes, P.A.; Martins, S.V.; Pinho, M.S.; Alfaia, C.M.; Fontes, C.M.G.A.; Rodrigues, P.O.; Morais, G.S.L.; et al. "Diet supplementation with the cis-9,trans-11 conjugated linoleic acid isomer affects the size of adipocytes in Wistar rats". Nutrition Research 28 7 (2008): 480-486. http://www.scopus.com/inward/record.url?eid=2-s2.0-44949151528&partnerID=MN8TOARS.
    10.1016/j.nutres.2008.04.002
  133. Guerreiro, C.I.P.D.; Ribeiro, T.; Ponte, P.I.P.; Lordelo, M.M.S.; Falcão, L.; Freire, J.P.B.; Ferreira, L.M.A.; Prates, J.A.M.; Fontes, C.M.G.A.. "Role of a family 11 carbohydrate-binding module in the function of a recombinant cellulase used to supplement a barley-based diet for broiler chickens". British Poultry Science 49 4 (2008): 446-454. http://www.scopus.com/inward/record.url?eid=2-s2.0-49649099975&partnerID=MN8TOARS.
    10.1080/00071660802216676
  134. Ponte, P.I.P.; Prates, J.A.M.; Crespo, J.P.; Crespo, D.G.; Mourão, J.L.; Alves, S.P.; Bessa, R.J.B.; et al. "Restricting the intake of a cereal-based feed in free-range-pastured poultry: Effects on performance and meat quality". Poultry Science 87 10 (2008): 2032-2042. http://www.scopus.com/inward/record.url?eid=2-s2.0-53349128421&partnerID=MN8TOARS.
    10.3382/ps.2007-00522
  135. Pinheiro, B.A.; Proctor, M.R.; Martinez-Fleites, C.; Prates, J.A.M.; Mon, V.A.; Davies, G.J.; Bayer, E.A.; et al. "The Clostridium cellulolyticum dockerin displays a dual binding mode for its cohesin partner". Journal of Biological Chemistry 283 26 (2008): 18422-18430. http://www.scopus.com/inward/record.url?eid=2-s2.0-49649107283&partnerID=MN8TOARS.
    10.1074/jbc.M801533200
  136. Money, V.A.; Cartmell, A.; Guerreiro, C.I.P.D.; Ducros, V.M.-A.; Fontes, C.M.G.A.; Gilbert, H.J.; Davies, G.J.. "Probing the ß-1,3:1,4 glucanase, CtLic26A, with a thio-oligosaccharide and enzyme variants". Organic and Biomolecular Chemistry 6 5 (2008): 851-853. http://www.scopus.com/inward/record.url?eid=2-s2.0-43249124256&partnerID=MN8TOARS.
    10.1039/b719288f
  137. Ponte, P.I.P.; Rosado, C.M.C.; Crespo, J.P.; Crespo, D.G.; Mourão, J.L.; Chaveiro-Soares, M.A.; Brás, J.L.A.; et al. "Pasture intake improves the performance and meat sensory attributes of free-range broilers". Poultry Science 87 1 (2008): 71-79. http://www.scopus.com/inward/record.url?eid=2-s2.0-37849041970&partnerID=MN8TOARS.
    10.3382/ps.2007-00147
  138. Ahmed, S.; Fontes, C.M.G.A.; Goyal, A.. "Recombinant lichenase from Clostridium thermocellum binds glucomannan but not to lichenan: Analysis by affinity electrophoresis". Annals of Microbiology 58 4 (2008): 723-725. http://www.scopus.com/inward/record.url?eid=2-s2.0-58149501660&partnerID=MN8TOARS.
  139. Najmudin, S.; Pinheiro, B.A.; Romão, M.J.; Prates, J.A.M.; Fontes, C.M.G.A.. "Purification, crystallization and crystallographic analysis of Clostridium thermocellum endo-1,4-ß-D-xylanase 10B in complex with xylohexaose". Acta Crystallographica Section F: Structural Biology and Crystallization Communications 64 8 (2008): 715-718. http://www.scopus.com/inward/record.url?eid=2-s2.0-49249121067&partnerID=MN8TOARS.
    10.1107/S1744309108019696
  140. Alfaia, C.P.M.; Castro, M.L.F.; Martins, S.I.V.; Portugal, A.P.V.; Alves, S.P.A.; Fontes, C.M.G.A.; Bessa, R.J.B.; Prates, J.A.M.. "Influence of slaughter season and muscle type on fatty acid composition, conjugated linoleic acid isomeric distribution and nutritional quality of intramuscular fat in Arouquesa-PDO veal". Meat Science 76 4 (2007): 787-795. http://www.scopus.com/inward/record.url?eid=2-s2.0-34247529038&partnerID=MN8TOARS.
    10.1016/j.meatsci.2007.02.023
  141. Carvalho, A.L.; Dias, F.M.V.; Nagy, T.; Prates, J.A.M.; Proctor, M.R.; Smith, N.; Bayer, E.A.; et al. "Evidence for a dual binding mode of dockerin modules to cohesins". Proceedings of the National Academy of Sciences of the United States of America 104 9 (2007): 3089-3094. http://www.scopus.com/inward/record.url?eid=2-s2.0-33847622467&partnerID=MN8TOARS.
    10.1073/pnas.0611173104
  142. Alfaia, C.M.M.; Ribeiro, P.J.L.C.; Trigo, M.J.P.; Alfaia, A.J.I.; Castro, M.L.F.; Fontes, C.M.G.A.; Bessa, R.J.B.; Prates, J.A.M.. "Irradiation effect on fatty acid composition and conjugated linoleic acid isomers in frozen lamb meat". Meat Science 77 4 (2007): 689-695. http://www.scopus.com/inward/record.url?eid=2-s2.0-35748964425&partnerID=MN8TOARS.
    10.1016/j.meatsci.2007.05.025
  143. Alfaia, C.M.M.; Castro, M.L.F.; Martins, S.I.V.; Portugal, A.P.V.; Alves, S.P.A.; Fontes, C.M.G.A.; Bessa, R.J.B.; Prates, J.A.M.. "Effect of slaughter season on fatty acid composition, conjugated linoleic acid isomers and nutritional value of intramuscular fat in Barrosã-PDO veal". Meat Science 75 1 (2007): 44-52. http://www.scopus.com/inward/record.url?eid=2-s2.0-33750037656&partnerID=MN8TOARS.
    10.1016/j.meatsci.2006.06.013
  144. Pires, V.M.R.; Fontes, C.M.A.; Ferreira, L.M.A.; Guerreiro, C.I.P.D.; Cunha, L.F.; Freire, J.P.B.. "The effect of enzyme supplementation on the true ileal digestibility of a lupin based diet for piglets". Livestock Science 109 1-3 (2007): 57-59. http://www.scopus.com/inward/record.url?eid=2-s2.0-34247526487&partnerID=MN8TOARS.
    10.1016/j.livsci.2007.01.060
  145. Martins, S.V.; Lopes, P.A.; Alfaia, C.M.; Ribeiro, V.S.; Guerreiro, T.V.; Fontes, C.M.G.A.; Castro, M.F.; Soveral, G.; Prates, J.A.M.. "Contents of conjugated linoleic acid isomers in ruminant-derived foods and estimation of their contribution to daily intake in Portugal". British Journal of Nutrition 98 6 (2007): 1206-1213. http://www.scopus.com/inward/record.url?eid=2-s2.0-36649008513&partnerID=MN8TOARS.
    10.1017/S0007114507781448
  146. Centeno, M.S.J.; Goyal, A.; Prates, J.A.M.; Ferreira, L.M.A.; Gilbert, H.J.; Fontes, C.M.G.A.. "Novel modular enzymes encoded by a cellulase gene cluster in Cellvibrio mixtus". FEMS Microbiology Letters 265 1 (2006): 26-34. http://www.scopus.com/inward/record.url?eid=2-s2.0-33750808632&partnerID=MN8TOARS.
    10.1111/j.1574-6968.2006.00464.x
  147. Bharali, S.; Purama, R.K.; Majumder, A.; Fontes, C.M.G.A.; Goyal, A.. "Molecular characteristics of a novel, recombinant glycoside hydrolase of family 26 from Clostridium thermocellum". Indian Journal of Microbiology 46 4 (2006): 371-378. http://www.scopus.com/inward/record.url?eid=2-s2.0-33847783640&partnerID=MN8TOARS.
  148. Taylor, E.J.; Gloster, T.M.; Turkenburg, J.P.; Vincent, F.; Brzozowski, A.M.; Dupont, C.; Shareck, F.; et al. "Structure and activity of two metal ion-dependent acetylxylan esterases involved in plant cell wall degradation reveals a close similarity to peptidoglycan deacetylases". Journal of Biological Chemistry 281 16 (2006): 10968-10975. http://www.scopus.com/inward/record.url?eid=2-s2.0-33744952521&partnerID=MN8TOARS.
    10.1074/jbc.M513066200
  149. Mestre Prates, J.A.; Gonçalves Quaresma, M.A.; Branquinho Bessa, R.J.; Andrade Fontes, C.M.G.; Mateus Alfaia, C.M.P.. "Simultaneous HPLC quantification of total cholesterol, tocopherols and ß-carotene in Barrosã-PDO veal". Food Chemistry 94 3 (2006): 469-477. http://www.scopus.com/inward/record.url?eid=2-s2.0-23044488879&partnerID=MN8TOARS.
    10.1016/j.foodchem.2005.01.021
  150. Alfaia, C.M.M.; Ribeiro, V.S.S.; Lourenço, M.R.A.; Quaresma, M.A.G.; Martins, S.I.V.; Portugal, A.P.V.; Fontes, C.M.G.A.; et al. "Fatty acid composition, conjugated linoleic acid isomers and cholesterol in beef from crossbred bullocks intensively produced and from Alentejana purebred bullocks reared according to Carnalentejana-PDO specifications". Meat Science 72 3 (2006): 425-436. http://www.scopus.com/inward/record.url?eid=2-s2.0-28844493518&partnerID=MN8TOARS.
    10.1016/j.meatsci.2005.08.012
  151. Martinez-Fleites, C.; Guerreiro, C.I.P.D.; Baumann, M.J.; Taylor, E.J.; Prates, J.A.M.; Ferreira, L.M.A.; Fontes, C.M.G.A.; Brumer, H.; Davies, G.J.. "Crystal structures of Clostridium thermocellum xyloglucanase, XGH74A, reveal the structural basis for xyloglucan recognition and degradation". Journal of Biological Chemistry 281 34 (2006): 24922-24933. http://www.scopus.com/inward/record.url?eid=2-s2.0-33747635388&partnerID=MN8TOARS.
    10.1074/jbc.M603583200
  152. Centeno, M.S.J.; Guerreiro, C.I.P.D.; Dias, F.M.V.; Morland, C.; Tailford, L.E.; Goyal, A.; Prates, J.A.M.; et al. "Galactomannan hydrolysis and mannose metabolism in Cellvibrio mixtus". FEMS Microbiology Letters 261 1 (2006): 123-132. http://www.scopus.com/inward/record.url?eid=2-s2.0-33745712447&partnerID=MN8TOARS.
    10.1111/j.1574-6968.2006.00342.x
  153. Alfaia, C.M.M.; Quaresma, M.A.G.; Castro, M.L.F.; Martins, S.I.V.; Portugal, A.P.V.; Fontes, C.M.G.A.; Bessa, R.J.B.; Prates, J.A.M.. "Fatty acid composition, including isomeric profile of conjugated linoleic acid, and cholesterol in Mertolenga-PDO beef". Journal of the Science of Food and Agriculture 86 13 (2006): 2196-2205. http://www.scopus.com/inward/record.url?eid=2-s2.0-33749600098&partnerID=MN8TOARS.
    10.1002/jsfa.2596
  154. Najmudin, S.; Guerreiro, C.I.P.D.; Carvalho, A.L.; Prates, J.A.M.; Correia, M.A.S.; Alves, V.D.; Ferreira, L.M.A.; et al. "Xyloglucan is recognized by carbohydrate-binding modules that interact with ß-glucan chains". Journal of Biological Chemistry 281 13 (2006): 8815-8828. http://www.scopus.com/inward/record.url?eid=2-s2.0-33646840847&partnerID=MN8TOARS.
    10.1074/jbc.M510559200
  155. Mourão, J.L.; Ponte, P.I.P.; Prates, J.A.M.; Centeno, M.S.J.; Ferreira, L.M.A.; Soares, M.A.C.; Fontes, C.M.G.A.. "Use of ß-glucanases and ß-1,4-xylanases to supplement diets containing alfalfa and rye for laying hens: Effects on bird performance and egg quality". Journal of Applied Poultry Research 15 2 (2006): 256-265. http://www.scopus.com/inward/record.url?eid=2-s2.0-33745675348&partnerID=MN8TOARS.
  156. Centeno, M.S.J.; Ponte, P.I.P.; Ribeiro, T.; Prates, J.A.M.; Ferreira, L.M.A.; Fontes, C.M.G.A.; Soares, M.C.; Gilbert, H.J.. "Galactanases and Mannanases Improve the Nutritive Value of Maize and Soybean Meal Based Diets for Broiler Chieks". Journal of Poultry Science 43 4 (2006): 344-350. http://www.scopus.com/inward/record.url?eid=2-s2.0-77952893903&partnerID=MN8TOARS.
    10.2141/jpsa.43.344
  157. Bharali, S.; Purama, R.K.; Majumder, A.; Fontes, C.M.G.A.; Goyal, A.. "Molecular cloning and biochemical properties of family 5 glycoside hydrolase of bi-functional cellulase from Clostridium thermocellum". Indian Journal of Microbiology 45 4 (2005): 317-321. http://www.scopus.com/inward/record.url?eid=2-s2.0-33744827101&partnerID=MN8TOARS.
  158. Carvalho, A.L.; Pires, V.M.R.; Gloster, T.M.; Turkenburg, J.P.; Prates, J.A.M.; Ferreira, L.M.A.; Romão, M.J.; et al. "Insights into the structural determinants of cohesin - Dockerin specificity revealed by the crystal structure of the type II cohesin from Clostridium thermocellum SdbA". Journal of Molecular Biology 349 5 (2005): 909-915. http://www.scopus.com/inward/record.url?eid=2-s2.0-20344372400&partnerID=MN8TOARS.
    10.1016/j.jmb.2005.04.037
  159. Najmudin, S.; Guerreiro, C.I.P.D.; Ferreira, L.M.A.; Romão, M.J.C.; Fontes, C.M.G.A.; Prates, J.A.M.. "Overexpression, purification and crystallization of the two C-terminal domains of the bifunctional cellulase ctCel9D-Cel44A from Clostridium thermocellum". Acta Crystallographica Section F: Structural Biology and Crystallization Communications 61 12 (2005): 1043-1045. http://www.scopus.com/inward/record.url?eid=2-s2.0-33744503690&partnerID=MN8TOARS.
    10.1107/S1744309105035670
  160. Tarbouriech, N.; Prates, J.A.M.; Fontes, C.M.G.A.; Davies, G.J.. "Molecular determinants of substrate specificity in the feruloyl esterase module of xylanase 10B from Clostridium thermocellum". Acta Crystallographica Section D: Biological Crystallography 61 2 (2005): 194-197. http://www.scopus.com/inward/record.url?eid=2-s2.0-21444453248&partnerID=MN8TOARS.
    10.1107/S0907444904029695
  161. Taylor, E.J.; Goyal, A.; Guerreiro, C.I.P.D.; Prates, J.A.M.; Money, V.A.; Ferry, N.; Morland, C.; et al. "How family 26 glycoside hydrolases orchestrate catalysis on different polysaccharides: Structure and activity of a Clostridium thermocellum lichenase, CtLic26A". Journal of Biological Chemistry 280 38 (2005): 32761-32767. http://www.scopus.com/inward/record.url?eid=2-s2.0-25444476150&partnerID=MN8TOARS.
    10.1074/jbc.M506580200
  162. Carvalho, A.L.; Goyal, A.; Prates, J.A.M.; Bolam, D.N.; Gilbert, H.J.; Pires, V.M.R.; Ferreira, L.M.A.; et al. "The family 11 carbohydrate-binding module of Clostridium thermocellum Lic26A-Cel5E accommodates ß-1,4- and ß-1,3-1,4-mixed linked glucans at a single binding site". Journal of Biological Chemistry 279 33 (2004): 34785-34793. http://www.scopus.com/inward/record.url?eid=2-s2.0-4544354845&partnerID=MN8TOARS.
    10.1074/jbc.M405867200
  163. Pires, V.M.R.; Henshaw, J.L.; Prates, J.A.M.; Bolam, D.N.; Ferreira, L.M.A.; Fontes, C.M.G.A.; Henrissat, B.; et al. "The crystal structure of the family 6 carbohydrate binding module from Cellvibrio mixtus endoglucanase 5A in complex with oligosaccharides reveals two distinct binding sites with different ligand specificities". Journal of Biological Chemistry 279 20 (2004): 21560-21568. http://www.scopus.com/inward/record.url?eid=2-s2.0-2442709187&partnerID=MN8TOARS.
    10.1074/jbc.M401599200
  164. Pell, G.; Taylor, E.J.; Gloster, T.M.; Turkenburg, J.P.; Fontes, C.M.G.A.; Ferreira, L.M.A.; Nagy, T.; et al. "The Mechanisms by Which Family 10 Glycoside Hydrolases Bind Decorated Substrates". Journal of Biological Chemistry 279 10 (2004): 9597-9605. http://www.scopus.com/inward/record.url?eid=2-s2.0-10744230264&partnerID=MN8TOARS.
    10.1074/jbc.M312278200
  165. Henshaw, J.L.; Bolam, D.N.; Pires, V.M.R.; Czjzek, M.; Henrissat, B.; Ferreira, L.M.A.; Fontes, C.M.G.A.; Gilbert, H.J.. "The family 6 carbohydrate binding module CmCBM6-2 contains two ligand-binding sites with distinct specificities". Journal of Biological Chemistry 279 20 (2004): 21552-21559. http://www.scopus.com/inward/record.url?eid=2-s2.0-2442681858&partnerID=MN8TOARS.
    10.1074/jbc.M401620200
  166. Ponte, P.I.P.; Mendes, I.; Quaresma, M.; Aguiar, M.N.M.; Lemos, J.P.C.; Ferreira, L.M.A.; Soares, M.A.C.; et al. "Cholesterol levels and sensory characteristics of meat from broilers consuming moderate to high levels of alfalfa". Poultry Science 83 5 (2004): 810-814. http://www.scopus.com/inward/record.url?eid=2-s2.0-3042780321&partnerID=MN8TOARS.
  167. Fontes, C.M.G.A.; Ponte, P.I.P.; Reis, T.C.; Soares, M.C.; Gama, L.T.; Dias, F.M.V.; Ferreira, L.M.A.. "A family 6 carbohydrate-binding module potentiates the efficiency of a recombinant xylanase used to supplement cereal-based diets for poultry". British Poultry Science 45 5 (2004): 648-656. http://www.scopus.com/inward/record.url?eid=2-s2.0-9944226863&partnerID=MN8TOARS.
    10.1080/00071660400006362
  168. Dias, F.M.V.; Vincent, F.; Pell, G.; Prates, J.A.M.; Centeno, M.S.J.; Tailford, L.E.; Ferreira, L.M.A.; et al. "Insights into the molecular determinants of substrate specificity in glycoside hydrolase family 5 revealed by the crystal structure and kinetics of Cellvibrio mixtus mannosidase 5A". Journal of Biological Chemistry 279 24 (2004): 25517-25526. http://www.scopus.com/inward/record.url?eid=2-s2.0-2942565674&partnerID=MN8TOARS.
    10.1074/jbc.M401647200
  169. Vincent, F.; Gloster, T.M.; Macdonald, J.; Morland, C.; Stick, R.V.; Dias, F.M.V.; Prates, J.A.M.; et al. "Common inhibition of both ß-glucosidases and ß-mannosidases by isofagomine lactam reflects different conformational itineraries for pyranoside hydrolysis". ChemBioChem 5 11 (2004): 1596-1599. http://www.scopus.com/inward/record.url?eid=2-s2.0-8844224107&partnerID=MN8TOARS.
    10.1002/cbic.200400169
  170. Ponte, P.I.P.; Ferreira, L.M.A.; Soares, M.A.C.; Aguiar, M.A.N.M.; Lemos, J.P.C.; Mendes, I.; Fontes, C.M.G.A.. "Use of cellulases and xylanases to supplement diets containing alfalfa for broiler chicks: Effects on bird performance and skin color". Journal of Applied Poultry Research 13 3 (2004): 412-420. http://www.scopus.com/inward/record.url?eid=2-s2.0-7044234887&partnerID=MN8TOARS.
  171. Dias, F.M.V.; Goyal, A.; Gilbert, H.J.; José A.M. Prates; Ferreira, L.M.A.; Fontes, C.M.G.A.. "The N-terminal family 22 carbohydrate-binding module of xylanase 10B of Clostridium themocellum is not a thermostabilizing domain". FEMS Microbiology Letters 238 1 (2004): 71-78. http://www.scopus.com/inward/record.url?eid=2-s2.0-4444244925&partnerID=MN8TOARS.
    10.1016/j.femsle.2004.07.019
  172. Ponte, P.I.P.; Ferreira, L.M.A.; Soares, M.A.C.; Gama, L.T.; Fontes, C.M.G.A.. "Xylanase inhibitors affect the action of exogenous enzymes used to supplement Triticum durum-based diets for broiler chicks". Journal of Applied Poultry Research 13 4 (2004): 660-666. http://www.scopus.com/inward/record.url?eid=2-s2.0-11844279771&partnerID=MN8TOARS.
  173. Carvalho, A.L.; Dias, F.M.V.; Prates, J.A.M.; Nagy, T.; Gilbert, H.J.; Davies, G.J.; Ferreira, L.M.A.; Romão, M.J.; Fontes, C.M.G.A.. "Cellulosome assembly revealed by the crystal structure of the cohesin-dockerin complex". Proceedings of the National Academy of Sciences of the United States of America 100 SUPPL. 2 (2003): 13809-13814. http://www.scopus.com/inward/record.url?eid=2-s2.0-0345564859&partnerID=MN8TOARS.
    10.1073/pnas.1936124100
  174. Emami, K.; Nagy, T.; Fontes, C.M.G.A.; Ferreira, L.M.A.; Gilbert, H.J.. "Evidence for temporal regulation of the two Pseudomonas cellulosa xylanases belonging to glycoside hydrolase family 11". Journal of Bacteriology 184 15 (2002): 4124-4133. http://www.scopus.com/inward/record.url?eid=2-s2.0-0036062315&partnerID=MN8TOARS.
    10.1128/JB.184.15.4124-4133.2002
  175. Nagy, T.; Emami, K.; Fontes, C.M.G.A.; Ferreira, L.M.A.; Humphry, D.R.; Gilbert, H.J.. "The membrane-bound a-glucuronidase from Pseudomonas cellulosa hydrolyzes 4-O-methyl-D-glucuronoxylooligosaccharides but not 4-O-methyl-D-glucuronoxylan". Journal of Bacteriology 184 17 (2002): 4925-4929. http://www.scopus.com/inward/record.url?eid=2-s2.0-0036716744&partnerID=MN8TOARS.
    10.1128/JB.184.17.4925-4929.2002
  176. Prates, J.A.M.; Tarbouriech, N.; Charnock, S.J.; Fontes, C.M.G.A.; Ferreira, L.M.A.; Davies, G.J.. "The structure of the feruloyl esterase module of xylanase 10B from Clostridium thermocellum provides insights into substrate recognition". Structure 9 12 (2001): 1183-1190. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035666321&partnerID=MN8TOARS.
    10.1016/S0969-2126(01)00684-0
  177. Czjzek, M.; Bolam, D.N.; Mosbah, A.; Allouch, J.; Fontes, C.M.G.A.; Ferreira, L.M.A.; Bornet, O.; et al. "The Location of the Ligand-binding Site of Carbohydrate-binding Modules That Have Evolved from a Common Sequence Is Not Conserved". Journal of Biological Chemistry 276 51 (2001): 48580-48587. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035930606&partnerID=MN8TOARS.
  178. Xie, H.; Gilbert, H.J.; Charnock, S.J.; Davies, G.J.; Williamson, M.P.; Simpson, P.J.; Raghothama, S.; et al. "Clostridium thermocellum Xyn10B carbohydrate-binding module 22-2: The role of conserved amino acids in ligand binding". Biochemistry 40 31 (2001): 9167-9176. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035822652&partnerID=MN8TOARS.
    10.1021/bi0106742
  179. Charnock, S.J.; Bolam, D.N.; Turkenburg, J.P.; Gilbert, H.J.; Ferreira, L.M.A.; Davies, G.J.; Fontes, C.M.G.A.. "The X6 'thermostabilizing' domains of xylanases are carbohydrate-binding modules: Structure and biochemistry of the Clostridium thermocellum X6b domain". Biochemistry 39 17 (2000): 5013-5021. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034595226&partnerID=MN8TOARS.
    10.1021/bi992821q
  180. Fontes, C.M.G.A.; Gilbert, H.J.; Hazlewood, G.P.; Clarke, J.H.; Prates, J.A.M.; McKie, V.A.; Nagy, T.; Fernandes, T.H.; Ferreira, L.M.A.. "A novel Cellvibrio mixtus family 10 xylanase that is both intracellular and expressed under non-inducing conditions". Microbiology 146 8 (2000): 1959-1967. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033900331&partnerID=MN8TOARS.
  181. Fernandes, A.C.; Fontes, C.M.G.A.; Gilbert, H.J.; Hazlewood, G.P.; Fernandes, T.H.; Ferreira, L.M.A.. "Homologous xylanases from Clostridium thermocellum: Evidence for bi-functional activity, synergism between xylanase catalytic modules and the presence of xylan-binding domains in enzyme complexes". Biochemical Journal 342 1 (1999): 105-110. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033567339&partnerID=MN8TOARS.
    10.1042/0264-6021:3420105
  182. Fontes, C.M.G.A.; Ali, S.; Gilbert, H.J.; Hazlewood, G.P.; Hirst, B.H.; Hall, J.. "Bacterial xylanase expression in mammalian cells and transgenic mice". Journal of Biotechnology 72 1-2 (1999): 95-101. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033049785&partnerID=MN8TOARS.
    10.1016/S0168-1656(99)00098-X
  183. Fontes, C.M.G.A.; Clarke, J.H.; Hazlewood, G.P.; Fernandes, T.H.; Gilbert, H.J.; Ferreira, L.M.A.. "Identification of tandemly repeated type VI cellulose-binding domains in an endoglucanase from the aerobic soil bacterium Cellvibrio mixtus". Applied Microbiology and Biotechnology 49 5 (1998): 552-559. http://www.scopus.com/inward/record.url?eid=2-s2.0-0031832318&partnerID=MN8TOARS.
    10.1007/s002530051212
  184. Fontes, C.M.G.A.; Hall, J.; Hazlewood, G.P.; Hirst, B.H.; Gilbert, H.J.. "The role of non-catalytic xylanase domains in protein thermostabilization". FASEB Journal 11 9 (1997): http://www.scopus.com/inward/record.url?eid=2-s2.0-33750144395&partnerID=MN8TOARS.
  185. Ali, S.; Fontes, C.M.G.A.; Hazlewood, G.P.; Hirst, B.H.; Clark, A.J.; Gilbert, H.J.; Hall, J.. "Co-integration and expression of bacterial and genomic transgenes in the pancreatic and intestinal tissues of transgenic mice". Gene 202 1-2 (1997): 203-208. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030779596&partnerID=MN8TOARS.
    10.1016/S0378-1119(97)00328-4
  186. Fontes, C.M.G.A.; Clarke, J.H.; Hazlewood, G.P.; Fernandes, T.H.; Gilbert, H.J.; Ferreira, L.M.A.. "Possible roles for a non-modular, thermostable and proteinase-resistant cellulase from the mesophilic aerobic soil bacterium Cellvibrio mixtus". Applied Microbiology and Biotechnology 48 4 (1997): 473-479. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030679609&partnerID=MN8TOARS.
    10.1007/s002530051082
  187. Cellvibrio Mixtus, L.M.A.; Ferreira, H.J.; Gilbert, G.P.; Fernandes, T.H.; Fontes, C.M.G.A.. "Molecular characterisation of a multi-domain cellulase from". FASEB Journal 11 9 (1997): http://www.scopus.com/inward/record.url?eid=2-s2.0-33750157962&partnerID=MN8TOARS.
  188. Clarke, J.H.; Davidson, K.; Gilbert, H.J.; Fontes, C.M.G.A.; Hazlewood, G.P.. "A modular xylanase from mesophilic Cellulomonas fimi contains the same cellulose-binding and thermostabilizing domains as xylanases from thermophilic bacteria". FEMS Microbiology Letters 139 1 (1996): 27-35. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030012798&partnerID=MN8TOARS.
    10.1016/0378-1097(96)00101-2
  189. Fontes, C.M.G.A.; Hazlewood, G.P.; Morag, E.; Hall, J.; Hirst, B.H.; Gilbert, H.J.. "Evidence for a general role for non-catalytic thermostabilizing domains in xylanases from thermophilic bacteria". Biochemical Journal 307 1 (1995): 151-158. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028949132&partnerID=MN8TOARS.
  190. Fontes, C.M.G.A.; Hall, J.; Hirst, B.H.; Hazlewood, G.P.; Gilbert, H.J.. "The resistance of cellulases and xylanases to proteolytic inactivation". Applied Microbiology and Biotechnology 43 1 (1995): 52-57. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028956723&partnerID=MN8TOARS.
    10.1007/s002530050369
Activities

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2017 - 2021 Improving the nutritional value of microalgae for feeding pigs through the use of novel enzymes
Co-supervisor
 Ciências Veterinárias (PhD)
Universidade de Lisboa, Portugal
2015 - 2019 High-Throughput (HTP) production and characterization of Carbohydrate-Active enZYmes (CAZYmes) for animal nutrition
Supervisor
 Ciências Veterinárias (PhD)
Universidade de Lisboa, Portugal
2013 - 2017 Divergent cellulosome architecture in rumen bacteria: structure and function studies in cohesion-dockerin complexes of Ruminococcus flavefaciens
Supervisor
 Ciências Veterinárias (PhD)
Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
2012 - 2016 Development of a novel platform for high-throughput gene design and artificial gene synthesis to produce large libraries of recombinant venom peptides for drug discovery
Supervisor
 Ciências Veterinárias (PhD)
Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
2011 - 2015 Structure and Function of novel Carbohydrate-Active Enzymes (CAZymes) and Carbohydrate-Binding Modules (CBMs) involved in Plant Cell Wall degradation
Supervisor
 Ciências Veterinárias (PhD)
Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
2011 - 2015 Structure and function relationships in novel cohesin dockerin complexes
Supervisor
 Ciências Veterinárias (PhD)
Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
2009 - 2013 Genetic and metabolic regulation of fatty acid deposition in autochthonous bovine breeds with distinct genetic background
Co-supervisor
 Ciências Veterinárias (PhD)
Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
2009 - 2013 Discovering novel Carbohydrate-Active Enzymes in the cellulosome of anaerobic bacteria bacteria
Supervisor
 Ciências Veterinárias (PhD)
Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
2008 - 2012 Structure and function relationships in novel cellulosomal enzymes and cohesin-dockerin complexes
Supervisor
 Ciências Veterinárias (PhD)
Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
2006 - 2010 Novel Insight into the Mechanism of cellulosome assembly and plant cell wall hydrolysis in anaerobic bacteria
Supervisor
 Ciências Veterinárias (PhD)
Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
2006 - 2010 Structural and functional insights into the role of Carbohydrate Esterases and Carbohydrate-Binding Modules in plant cell wall hydrolysis
Co-supervisor
 Ciências Veterinárias (PhD)
Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
2005 - 2009 Rational use of dietary enzymes and lipids to improve broiler performance and meat quality
Supervisor
 Ciências Veterinárias (PhD)
Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
2004 - 2008 Effect of pasture biomass intake on growth performance and meat quality of free-range broilers
Supervisor
 Ciências Veterinárias (PhD)
Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
2004 - 2008 Molecular mechanisms of plant cell wall hydrolysis: developing novel biotechnological applications for carbohydrate-binding modules
Supervisor
 Ciências Veterinárias (PhD)
Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
2004 - 2008 Structure and function of non-catalytic modules involved in plant cell hydrolysis: exogenous enzymes to improve the nutritive value of a Lupinus albus based diet for piglets
Supervisor
 Ciências Veterinárias (PhD)
Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
2003 - 2007 Estrutura e função de hemicelulases e complexos coesina-doquerina: implicações relativas à hidrólise de carbohidratos estruturais
Supervisor
 Ciências Veterinárias (PhD)
Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
2003 - 2007 Caracterização e modificação genética de polissacaridases tendo em vista a sua utilização na alimentação de animais monogástricos
Co-supervisor
 Ciências Veterinárias (PhD)
Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal