Aldino Viegas (C411-0563-3D6B)

Curriculum Vitae

Aldino Viegas

I graduated in Biochemistry in Faculdade de Ciências da Univ. de Lisboa and I received my Ph.D. in Structural Biochemistry in 2012 by Faculdade de Ciências e Tecnologia da Univ. Nova de Lisboa (FCT-NOVA), having been awarded the António Xavier Prize, for the Best National Thesis in the field of NMR (2013). My research focused on the study of protein-ligand interactions and protein structure by NMR spectroscopy, X-ray crystallography, molecular biology and molecular docking. In 2012 I attained a position as a Scientific Officer at Fundação para a Ciência e a Tecnologia, and, in Sep. 2013, to embrace a new scientific challenge on the study of membrane proteins (MP), I took a research position in Germany (HHU Duesseldorf) to study MP structure and function by NMR. Shortly after, I was awarded a Marie Sklodowska-Curie Individual Fellowship to study the Epidermal Growth Factor Receptor (EGFR). This granted me strong skills in the field of MP NMR, membrane mimetics (nanodiscs) and pulse sequence development (UTOPIA-NMR). The methods developed and the results obtained advanced both the field of NMR for the study of MPs, and the current knowledge on key aspects of EGFR signaling. During this period, I was involved in teaching and co-supervision of master and Ph.D. students and had the opportunity to collaborate with experts in areas from chemistry, biochemistry to NMR engineering. Since Nov. 2017, I am working as a researcher in FCT-NOVA and my research is focused on the integration of NMR with other structural biology techniques to study protein-ligand interactions in complex systems and the effect of charged metabolites on protein folding and stability. In 2020 I was a awarded an Assistant Researcher contract (2020.00043.CEECIND) in the 2020 Individual Call to Scientific Employment Stimulus to study the molecular basis for ligand recognition of the human norepinephrine transporter. To complement this contract, in 2021 I was awarded an exploratory project (EXPL/BIA-BQM/0011/2021) to study ligand interaction and allosteric modulation of the same transporter.

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Identification

Personal identification

Full name: Aldino Viegas

Citation names

Viegas, Aldino

Author identifiers

Ciência ID: C411-0563-3D6B
ORCID iD: 0000-0003-1733-136X
Google Scholar ID: WtoAvCEAAAAJ&hl
Researcher Id: A-1889-2011
Scopus Author Id: 24167082900

Email addresses

a.viegas@fct.unl.pt (Professional)

Telephones

Telephone

212948300 Ext.: 10999 (Professional)

Addresses

UCIBIO, Departamento de Quimica, Gabinete 303, FCT-UNL, 2829-516, Caparica, Almada, Portugal (Professional)

Websites

https://www.requimte.pt/ucibio/people/aldino-viegas (Scholar)
https://www.linkedin.com/in/aviegas/ (Social media)
https://orcid.org/0000-0003-1733-136X (Professional)
https://aldinov.wixsite.com/aldinoviegas (Personal)
https://twitter.com/AldinoViegas (Social media)

Knowledge fields

Natural sciences

Languages

Language	Speaking	Reading	Writing	Listening
Portuguese	Advanced (C1)	Advanced (C1)	Advanced (C1)	Advanced (C1)
English	Advanced (C1)	Advanced (C1)	Advanced (C1)	Advanced (C1)
Spanish; Castilian	Advanced (C1)	Advanced (C1)	Beginner (A1)	Advanced (C1)
French	Beginner (A1)	Beginner (A1)	Beginner (A1)	Beginner (A1)
German	Beginner (A1)	Beginner (A1)	Beginner (A1)	Beginner (A1)

Education

	Degree	Classification
2008/03/01 - 2012/05/28 Concluded	Structural Biochemistry (Doutoramento) Major in Structural Biochemistry Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal "Molecular Determinants of Ligand Specificity in Carbohydrate-Binding Modules: an NMR and X-ray crystallography integrated study" (THESIS/DISSERTATION)	Aprovado por unanimidade
2001 - 2006 Concluded	Biochemistry (Licenciatura) Universidade de Lisboa Faculdade de Ciências, Portugal "Determinantes Moleculares de especificidade nos CBMs da família 11" (THESIS/DISSERTATION)	15

Affiliation

Science

	Category Host institution	Employer
2021 - Current	Research Assistant (Research)	Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
	Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2017/11/01 - 2021	Researcher (Research)	Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
	Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2013/09 - 2017/03/31	Researcher (Research)	Heinrich-Heine-Universität Düsseldorf, Germany
	Heinrich-Heine-Universität Düsseldorf, Germany
2013/04/15 - 2013/09/13	Science and Technology Management	Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
	Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2008/03 - 2012/05	Researcher (Research)	Universidade Nova de Lisboa, Portugal

2006/10 - 2007/09	Research Trainee (Research)	Universidade Nova de Lisboa, Portugal

Others

	Category Host institution	Employer
2012/05/15 - 2013/04/15	Scientific Officer	Fundação para a Ciência e a Tecnologia, Portugal
	Fundação para a Ciência e a Tecnologia, Portugal

Projects

Grant

	Designation	Funders
2022/04/01 - 2024/03/31	Biochemical, functional and structural studies of the human norepinephrine transporter. 2021.09576.CBM Principal investigator REQUIMTE Unidade de Ciências Biomoleculares Aplicadas, Portugal Heinrich-Heine-Universität Düsseldorf, Germany	Fundação para a Ciência e a Tecnologia Deutscher Akademischer Austauschdienst Ongoing
2022/01/01 - 2023/06/30	NETexplore - Understanding ligand interaction and allosteric modulation of the norepinephrine transporter (NET) EXPL/BIA-BQM/0011/2021 Principal investigator Associação para a Inovação e Desenvolvimento da FCT, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2013/07 - 2016/06	Modern Structural Biology: Resources for the advancement of in-house X-ray Crystallography 125363 Provided by PTCRIS: 125363 PhD Student Fellow	Fundação para a Ciência e a Tecnologia
2013/07 - 2016/06	NMR Net - National Facility for Nuclear Magnetic Resonance: from Molecular Structure and Dynamics to Protein Function, Cell Physiology, and Metabolomics 127013 Provided by PTCRIS: 127013 PhD Student Fellow	Fundação para a Ciência e a Tecnologia
2010/01 - 2013/06	Structure and function of novel Glycoside Hydrolases and Carbohydrate-Binding Modules of Clostridium thermocellum cellulosome 103980 Provided by PTCRIS: 103980 PhD Student Fellow	Fundação para a Ciência e a Tecnologia
2008/03 - 2012/03	Molecular Determinants of Ligand Specificity in Carbohydrate-Binding Modules: an NMR and X-ray crystallography integrated study SFRH/BD/35992/2007 Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2008/01 - 2011/09	A combined approach of X-ray Crystallography, NMR and Computational Chemistry for the study of the molecular interactions that define the ligand specificity in cellulosomal Carbohydrate Binding Modules 68286 Provided by PTCRIS: 68286 PhD Student Fellow	Fundação para a Ciência e a Tecnologia

Contract

	Designation	Funders
2021 - Current	NETfix - Molecular basis for ligand recognition of the human norepinephrine transporter 2020.00043.CEECIND Principal investigator Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2021/01/01 - 2025/12/31	Institute for Health and Bioeconomy LA/P/0140/2020 10.54499/LA/P/0140/2020 Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal Unidade de Ciências Biomoleculares Aplicadas, Portugal Associação para a Inovação e Desenvolvimento da FCT, Portugal Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal INESC Microsistemas e Nanotecnologias, Portugal Universidade NOVA de Lisboa, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2019/01/01 - 2019/12/31	Unidade de Ciências Biomoleculares Aplicadas UID/Multi/04378/2019 Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2014/04/01 - 2017/03/31	Marie Sklodowska-Curie Individual Fellowship (EGFR-Activ) H2020-MSCA-IF-2014_660258 Researcher Heinrich-Heine-Universität Düsseldorf, Germany	European Commission Concluded
2013/07/01 - 2016/06/30	NMR Net - Centro Nacional de Ressonância Magnética Nuclear: desde a Estrutura e Dinâmica Moleculares à Função Proteica, Fisiologia Celular e Metabolómica RECI/BBB-BQB/0230/2012 Universidade NOVA de Lisboa Faculdade de Ciências e Tecnologia, Portugal Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica António Xavier, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2011/01/01 - 2012/12/31	Strategic Project - LA 6 - 2011-2012 PEst-C/EQB/LA0006/2011 Universidade NOVA de Lisboa Faculdade de Ciências e Tecnologia, Portugal Universidade do Porto Instituto de Ciências Tecnologias e Agroambiente, Portugal Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal	Fundação para a Ciência e a Tecnologia Concluded

Other

	Designation	Funders
2020/01/01 - 2023/12/31	Applied Molecular Biosciences Unit UIDB/04378/2020 10.54499/UIDB/04378/2020 Universidade do Porto, Portugal Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal Universidade do Porto Instituto de Ciências Tecnologias e Agroambiente, Portugal Unidade de Ciências Biomoleculares Aplicadas, Portugal Associação para a Inovação e Desenvolvimento da FCT, Portugal Universidade NOVA de Lisboa, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2006 - 2007	Molecular Determinants of Ligand Specificity in Family 11 Carbohydrate-Binding Modules: an X-ray, NMR and computational chemistry combined approach Projeto Reqmol Other Universidade Nova de Lisboa, Portugal	Concluded
2006 - 2007	The Solution Structure Of Hirsutellin A: Structure-Function Relationship of Ribotoxins Convénio GRICES-CSIC Other Universidade Nova de Lisboa, Portugal	Concluded

Outputs

Publications

Book chapter	Brás, Joana L.A.; Carvalho, Ana Luísa; Viegas, Aldino; Najmudin, Shabir; Alves, Victor D.; Prates, José A.M.; Ferreira, Luís M.A.; et al. "Escherichia coli Expression, Purification, Crystallization, and Structure Determination of Bacterial Cohesin–Dockerin Complexes". In Cellulases, 395-415. California: Elsevier, 2012. Published • 10.1016/B978-0-12-415931-0.00021-5 Viegas, Aldino; Macedo, Anjos L.; Cabrita, Eurico J.. "Ligand-Based Nuclear Magnetic Resonance Screening Techniques". In Ligand-Macromolecular Interactions in Drug Discovery, 81-100. Totowa, NJ: Humana Press, 2010. Published • 10.1007/978-1-60761-244-5_6
Conference poster	Vilela-Alves, G; Rebelo Manuel, Rita; Viegas, Aldino; Philippe Carpentier; Frederic Biaso; Bruno Guigliarelli; Inês A. Cardoso Pereira; Maria Joao Romao; Mota, Cristiano. Corresponding author: Mota, Cristiano. "O2 damage on Mo/W Formate dehydrogenases". Paper presented in 10th National Meeting of Portuguese Users of Synchrotron Radiation, 2024. Vilela-Alves, G; Oliveira, Ana Rita; Rebelo Manuel, Rita; Viegas, Aldino; Kateryna Klymanska; Philippe Carpentier; Christophe Léger; et al. Corresponding author: Mota, Cristiano. "Unveiling O2 damage on Mo/W Formate dehydrogenases and their innate protection mechanism". Paper presented in Instruct-ERIC Biennial Structural Biology Conference 2024, 2024. Engrola, Filipa; Correia, Márcia; Viegas, Aldino; Romão, Maria; Santos-Silva, Teresa. "Unveiling the interaction of arsenite oxidase with electron parterns – a structural and biophysical approach". Paper presented in CCP4 Study Weekend 2024: Decision making in MX - how to be a productive structural biologist, 2024. Engrola, Filipa; Correia, Márcia; Cameron Watson; Joanne M. Santini; Viegas, Aldino; Maria Joao Romao; Santos-Silva, Teresa. "A biophysical and structural approach to shed light on arsenite oxidase reaction mechanism". Paper presented in 9th National Meeting of Synchrotron Radiation Users - ENURS 2023, Portugal, 2023. Engrola, Filipa; Correia, Márcia; Cameron Watson; Joanne M. Santini; Viegas, Aldino; Maria Joao Romao; Santos-Silva, Teresa. "Arsenite oxidase interaction with substrates and partners: a structural and biophysical characterization". Paper presented in 8th International School of Biological Crystallization - ISBC 2023, Spain, 2023. Engrola, Filipa; Lora Denson; Pinheiro, Benedita; Ci Chu; Santos-Silva, Teresa; Manuel Etzkorn; Viegas, Aldino. "Production of human norepinephrine transporter - hNET - in insect cells". Paper presented in Joint Instruct-iNEXT Course: Integrating Structural Biology Techniques, 2022. Engrola, Filipa; Correia, Márcia; Viegas, Aldino; Maria Joao Romao; Santos-Silva, Teresa. "Arsenite oxidase – a structural and biophysical study from the reaction intermediates to the electron acceptors". Paper presented in Meeting with UCIBIO Scientific Advisory Board, 2022. Silva, Micael S.; Viegas, Aldino; Philip O'Toole; Figueiredo, Angelo M; Cabrita, Eurico J.. "Choline glutamate modulates protein folding and stability: an NMR view". Paper presented in 2020 CCPN Conference online, 2020. Silva, Micael S.; O'Toole, Philip; Viegas, Aldino; Figueiredo, Angelo M.; Cabrita, Eurico J.. "Modulation of protein (de)stabilisation by ionic liquids: an NMR view". Paper presented in 21th ISMAR - 15th EUROMAR joint conference, 2019. Silva, Micael S.; Viegas, Aldino; Figueiredo, Angelo M.; Cabrita, Eurico J.. "Atomistic picture of charged metabolite – protein interaction with repercussions on protein stabilization". Paper presented in 9th ITQB NOVA PhD Student’s Meeting, 2018. Silva, Micael S.; O’Toole, Philip; Viegas, Aldino; Figueiredo, Angelo M.; Cabrita, Eurico J.. "Tuning protein stability with ionic liquids: investigation of destabilizing vs stabilizing effects". Paper presented in 28th ICMRBS - International Conference on Magnetic Resonance in Biological Systems, 2018. Silva, Micael S.; Viegas, Aldino; Figueiredo, Angelo M.; Cabrita, Eurico J.. "Discriminating the effect of charged metabolite interactions on protein structure and stability". Paper presented in 6th International Iberian Biophysics Congress and X Ibero-American Congress of Biophysics, 2018. Silva, Micael S.; Viegas, Aldino; Figueiredo, Angelo M.; Cabrita, Eurico J.. "Discerning the effect of charged metabolite interactions on proteins". Paper presented in VIII Ibero-American NMR Meeting, 2018. Viegas, Aldino; Freire, Filipe; Sardinha, João; Carvalho, Ana Luísa; Fontes, CMGA; Maria Joao Romao; Macedo, AL. "Solution Structure, Dynamics and Binding Studies of CtCBM11". Paper presented in 2nd PTNMR Meeting, 2012. Pimenta J; Soares Rui; Viegas, Aldino; Cabrita EJ; Fontes CA; Prates, JAM; Pereira RM. "NMR solution structure of the N-terminal Sequence (1-30) of ovine prion-like (Doppel) protein". Paper presented in 3rd EU/CCPN NMR Conference, 2011. Viegas, Aldino; Freire, Filipe; Carvalho, Ana Luísa; Maria Joao Romao; Cabrita, E. J.. "Insights into the mechanism of molecular selectivity and recognition by carbohydrate-binding modules from Clostridium thermocellum". Paper presented in 9th Carbohydrate Bioengineering Meeting, 2011. Viegas, Aldino; Freire, Filipe; Carvalho, Ana Luísa; Macedo, AL; Cabrita, E. J.. "The influence of Temperature and Binding on the Dynamics of CtCBM11". Paper presented in Conference CCPN Europa 2011 ‘Supporting best practice in biomolecular NMR’, 2011.
Journal article	Vilela-Alves, Guilherme; Manuel, Rita R.; Viegas, Aldino; Carpentier, Philippe; Biaso, Frédéric; Guigliarelli, Bruno; Pereira, Inês A. C.; Romão, Maria João; Mota, Cristiano. "Substrate-dependent oxidative inactivation of a W-dependent formate dehydrogenase involving selenocysteine displacement". Chemical Science (2024): http://dx.doi.org/10.1039/d4sc02394c. 10.1039/d4sc02394c Jan Borggräfe; Christoph G.W. Gertzen; Viegas, A; Holger Gohlke; Manuel Etzkorn. "The architecture of the 10-23 DNAzyme and its implications for DNA-mediated catalysis". The FEBS Journal (2022): http://dx.doi.org/10.1111/febs.16698. Published • 10.1111/febs.16698 Mohanraj Gopalswamy; Tobias Kroeger; David Bickel; Benedikt Frieg; Shahina Akter; Stephan Schott-Verdugo; Aldino Viegas; et al. "Biophysical and pharmacokinetic characterization of a small-molecule inhibitor of RUNX1/ETO tetramerization with anti-leukemic effects". Scientific Reports 12 1 (2022): http://dx.doi.org/10.1038/s41598-022-17913-6. Open access • 10.1101/2021.12.22.473911 Borggräfe, Jan; Victor, Julian; Rosenbach, Hannah; Viegas, Aldino; Gertzen, Christoph G. W.; Wuebben, Christine; Kovacs, Helena; et al. Corresponding author: Etzkorn, Manuel. "Time-resolved structural analysis of an RNA-cleaving DNA catalyst". Nature (2021): http://dx.doi.org/10.1038/s41586-021-04225-4. 10.1038/s41586-021-04225-4 Dubey, Abhinav; Stoyanov, Nikolay; Viennet, Thibault; Chhabra, Sandeep; Elter, Shantha; Borggräfe, Jan; Viegas, Aldino; et al. "Local deuteration enables NMR observation of methyl groups in proteins from eukaryotic and cell-free expression systems". Angewandte Chemie (2021): https://doi.org/10.1002/anie.202016070. Published • 10.1002/anie.202016070 Duarte, Marlene; Viegas, Aldino; Alves, Victor D.; Prates, José A.M.; Ferreira, Luís M.A.; Najmudin, Shabir; Cabrita, Eurico J.; et al. "A dual cohesin–dockerin complex binding mode in Bacteroides cellulosolvens contributes to the size and complexity of its cellulosome". Journal of Biological Chemistry 296 (2021): 100552. http://dx.doi.org/10.1016/j.jbc.2021.100552. Open access • Published • 10.1016/j.jbc.2021.100552 Viegas, Aldino; Dollinger, Peter; Verma, Neha; Kubiak, Jakub; Viennet, Thibault; Seidel, Claus A. M.; Gohlke, Holger; et al. "Structural and dynamic insights revealing how lipase binding domain MD1 of Pseudomonas aeruginosa foldase affects lipase activation". Scientific Reports 10 1 (2020): http://dx.doi.org/10.1038/s41598-020-60093-4. Open access • Published • 10.1038/s41598-020-60093-4 Diana Ribeiro; Viegas, Aldino; Virgínia M. R. Pires; João Medeiros-Silva; Pedro Bule; Wengang Chai; Filipa Marcelo; et al. "Molecular basis for the preferential recognition of ß1,3-1,4-Glucans by the Family 11 Carbohydrate-Binding Module from Clostridium thermocellum". The FEBS Journal 287 13 (2019): 2723-2743. http://dx.doi.org/10.1111/febs.15162. Published • 10.1111/febs.15162 Viegas, Aldino; Yin, Dongsheng M.; Borggräfe, Jan; Viennet, Thibault; Falke, Marcel; Schmitz, Anton; Famulok, Michael; Etzkorn, Manuel. "Molecular Architecture of a Network of Potential Intracellular EGFR Modulators: ARNO, CaM, Phospholipids, and the Juxtamembrane Segment". Structure 28 1 (2019): 54-62. http://dx.doi.org/10.1016/j.str.2019.11.001. Published • 10.1016/j.str.2019.11.001 Thibault Viennet; Stefanie Bungert-Plümke; Shantha Elter; Aldino Viegas; Christoph Fahlke; Manuel Etzkorn. "Reconstitution and NMR Characterization of the Ion-Channel Accessory Subunit Barttin in Detergents and Lipid-Bilayer Nanodiscs". Frontiers in Molecular Biosciences 6 (2019): https://doi.org/10.3389%2Ffmolb.2019.00013. 10.3389/fmolb.2019.00013 Viegas, Aldino; Viennet, Thibault; Yu, Tsyr-Yan; Schumann, Frank; Bermel, Wolfgang; Wagner, Gerhard; Etzkorn, Manuel. "UTOPIA NMR: activating unexploited magnetization using interleaved low-gamma detection". Journal of Biomolecular NMR 64 1 (2016): 9-15. http://link.springer.com/article/10.1007%2Fs10858-015-0008-7. Published • 10.1007/s10858-015-0008-7 Viennet, Thibault; Viegas, Aldino; Kuepper, Arne; Arens, Sabine; Gelev, Vladimir; Petrov, Ognyan; Grossmann, Tom N.; Heise, Henrike; Etzkorn, Manuel. "Selective Protein Hyperpolarization in Cell Lysates Using Targeted Dynamic Nuclear Polarization". Angewandte Chemie International Edition 55 36 (2016): 10746-10750. http://onlinelibrary.wiley.com/doi/10.1002/anie.201603205/abstract. Published • 10.1002/anie.201603205 Viegas, Aldino; Viennet, Thibault; Etzkorn, Manuel. "The power, pitfalls and potential of the nanodisc system for NMR-based studies". Biological Chemistry 397 12 (2016): 1335-1354. Published • 10.1515/hsz-2016-0224 Brás, Joana L. A.; Pinheiro, Benedita A.; Cameron, Kate; Cuskin, Fiona; Viegas, Aldino; Najmudin, Shabir; Bule, Pedro; et al. "Diverse specificity of cellulosome attachment to the bacterial cell surface". Scientific Reports 6 38292 (2016): 38292-38292. http://www.nature.com/articles/srep38292. Published • 10.1038/srep38292 Elter, Shantha; Raschle, Thomas; Arens, Sabine; Viegas, Aldino; Gelev, Vladimir; Etzkorn, Manuel; Wagner, Gerhard. "The Use of Amphipols for NMR Structural Characterization of 7-TM Proteins". The Journal of Membrane Biology 247 9-10 (2014): 957-964. Published • 10.1007/s00232-014-9669-5 Viegas, Aldino; Sardinha, João; Freire, Filipe; Duarte, Daniel; Carvalho, Ana; Fontes, Carlos; Romão, Maria; Macedo, Anjos; Cabrita, Eurico. "Solution Structure, Dynamics and Binding Studies of a Family 11 Carbohydrate-Binding Module from Clostridium thermocellum (CtCBM11)". Biochemical Journal 451 2 (2013): 289-300. Published • 10.1042/BJ20120627 Pimenta, Jorge; Sardinha, João; Marques, Carla C; Domingos, Ana; Baptista, Maria C; Barbas, João P; Martins, Ivo C; et al. "Inhibition of ovine in vitro fertilization by anti-Prt antibody: hypothetical model for Prt/ZP interaction". Reproductive Biology and Endocrinology 11 25 (2013): 1-11. Published • 10.1186/1477-7827-11-25 Pimenta, Jorge; Viegas, Aldino; Sardinha, João; Martins, Ivo C; Cabrita, Eurico J.; Fontes, Carlos M.G.A.; Prates, José A.; Pereira, Rosa M.L.N.. "NMR solution structure and SRP54M predicted interaction of the N-terminal sequence (1-30) of the ovine Doppel protein". Peptides 49 (2013): 32-40. http://www.sciencedirect.com/science/article/pii/S0196978113002842. Published • 10.1016/j.peptides.2013.08.013 Quintal, Susana; Viegas, Aldino; Erhardt, Stefan; Cabrita, Eurico J.; Farrell, Nicholas P.. "Platinated DNA Affects Zinc Finger Conformation. Interaction of a Platinated Single-Stranded Oligonucleotide and the C-Terminal Zinc Finger of Nucleocapsid Protein HIVNCp7". Biochemistry 51 8 (2012): 1752-1761. Published • 10.1021/bi201834g Estevão, Mónica S.; Carvalho, Luísa C.R.; Freitas, Marisa; Gomes, Ana; Viegas, Aldino; Manso, João; Erhardt, Stefan; et al. "Indole based cyclooxygenase inhibitors: Synthesis, biological evaluation, docking and NMR screening". European Journal of Medicinal Chemistry 54 54 (2012): 823-833. Published • 10.1016/j.ejmech.2012.06.040 Pimenta, Jorge; Domingos, Ana; Santos, Pedro; Marques, Carla C.; Cantante, Cátia; Santos, Ana; Barbas, João P.; et al. "Is prnt a Pseudogene? Identification of Ram Prt in Testis and Ejaculated Spermatozoa". PLoS ONE 7 8 (2012): e42957-e42957. Published • 10.1371/journal.pone.0042957 Viegas, Aldino; Manso, João; Nobrega, Franklin L.; Cabrita, Eurico J.. "Saturation-Transfer Difference (STD) NMR: A Simple and Fast Method for Ligand Screening and Characterization of Protein Binding". Journal of Chemical Education 88 7 (2011): 990-994. Published • 10.1021/ed101169t Viegas, Aldino; Manso, João; Corvo, Marta C.; Marques, M. Manuel B.; Cabrita, Eurico J.. "Binding of Ibuprofen, Ketorolac, and Diclofenac to COX-1 and COX-2 Studied by Saturation Transfer Difference NMR". Journal of Medicinal Chemistry 54 24 (2011): 8555-8562. Published • 10.1021/jm201090k Viegas, Aldino; Herrero-Galán, Elias; Oñaderra, Mercedes; Macedo, Anjos L.; Bruix, Marta. "Solution structure of hirsutellin A - new insights into the active site and interacting interfaces of ribotoxins". FEBS Journal 276 8 (2009): 2381-2390. Published • 10.1111/j.1742-4658.2009.06970.x Viegas, Aldino; Brás, Natércia F.; Cerqueira, Nuno M. F. S. A.; Fernandes, Pedro Alexandrino; Prates, José A. M.; Fontes, Carlos M. G. A.; Bruix, Marta; et al. "Molecular determinants of ligand specificity in family 11 carbohydrate binding modules - an NMR, X-ray crystallography and computational chemistry approach". FEBS Journal 275 10 (2008): 2524-2535. Published • 10.1111/j.1742-4658.2008.06401.x
Online resource	Pimenta, J.; Viegas, Aldino. Ovine Doppel Signal peptide (1-30) Solution NMR. 2012. https://www.rcsb.org/structure/2m1j.

Activities

Oral presentation

	Presentation title	Event name Host (Event location)
2023/03/11	Protein-Ligand Interactions by Saturation Transfer Difference NMR	5th G-NMR school Heinrich Heine University Düsseldorf (Düsseldorf, Germany)
2018/09/29	NMR Structural Studies in Drug Discovery and Development	UCIBIO Annual Meeting (Caparica, Portugal)
2018/04	The power, pitfalls and potential of the nanodisc system for NMR-based studies	COST ACTION CM1306 (Arrábida, Portugal)
2018/01/10	NMR Studies of Challenging Biological Systems	7th LAQV-UCIBIO Conference Cycle Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa (Caparica, Portugal)
2015	UTOPIA NMR: Recovering Lost Magnetization Using Interleaved Low-Gamma Detection	EUROMAR 2015 (Praga, Czech Republic)
2014	Time-optimized NMR spectroscopy using interleaved acquisition	VII GERMN Biennial Meeting / IV Iberian NMR Meeting / VI Iberoamerican NMR Meeting (Henares de Alcalá, Spain)
2014	Time-optimized NMR spectroscopy using interleaved acquisition	BioNMR-network-NRW meeting (Essen, Germany)
2012	NMR insights into the mechanism of molecular selectivity and recognition by carbohydrate-binding modules from Clostridium thermocellum	26th International Carbohydrate Symposium (Madrid, Spain)
2009	Revealing the Global Mechanism of Interaction of CtCBM11	12th NMR Users Meeting / 3rd Iberoamerican Meeting AUREMN (Rio de Janeiro, Brazil)

Supervision

	Thesis Title Role	Degree Subject (Type) Institution / Organization
2022/01 - Current	NMR study of the mechanism of interaction of ionic liquids with proteins Supervisor of José Miguel Fiadeiro Lucas	Programa Doutoral em RMN aplicado à Química, Materiais e Biociências (PhD) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2021 - Current	The human Aldehyde Oxidase (hAOX1) – unravelling drug metabolization Co-supervisor	Biochemistry (PhD) REQUIMTE Unidade de Ciências Biomoleculares Aplicadas, Portugal
2021/10 - 2023/06/01	Insights into the structure, kinetics and aggregation of a-synuclein by charged metabolites Supervisor of Fredi Brigham	MSc in Biochemistry for Health (Master) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2020/01/20 - 2020/06/30	Structural studies of alpha-Synuclein and N-acetylated alpha-Synuclein under the influence of common metabolites Co-supervisor	Bioquímica (Degree) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2019/01/28 - 2019/06/21	Estudos Estruturais da a-Sinucleína sob a Influência de Metabolitos Comuns Co-supervisor	Bioquímica (Degree) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal

Event participation

	Activity description Type of event	Event name Institution / Organization
2023/08/12 - 2023/08/15	Production of human norepinephrine transporter in insect cells Conference	The Brain in Flux - MSCA NeuroTrans Joint Satellite Meeting Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2022/04/04 - 2022/04/06	Attendance to the 3rd UK Workshop on Membrane Proteins Workshop	3rd UK Workshop on Membrane Proteins Biochemical Society, United Kingdom
2019/09/21 - 2019/09/21	miR-149 G-quadruplex recognition by the nucleolin protein in prostate cancer cells Meeting	PTNMR Meeting
2019/08/25 - 2019/08/30	Modulation of protein (de)stabilisation by Ionic Liquids: an NMR view Conference	EUROISMAR 2019 Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2019/08/25 - 2019/08/30	NMR and challenging biological systems Conference	EUROISMAR 2019 Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2014/09/19 - 2014/09/19	Time-optimized NMR spectroscopy using interleaved acquisition Meeting	BioNMR-network-NRW meeting Universität Duisburg-Essen, Germany
2012 - 2012	Insights into the mechanism of cellulosome attachment to the bacterial cell surface: the crystal structure of the type II cohesin-dockerin complex in C. thermocellum and B. cellulosolvens Meeting	1st National Meeting of Users of Synchrotron Radiation
2012 - 2012	Solution Structure, Dynamics and Binding Studies of CtCBM11 Congress	2nd PTNMR Meeting – NMR in Portugal: an overview
2012 - 2012	Protein-ligand interactions studied by STD-NMR Meeting	2nd PTNMR Meeting – NMR in Portugal: an overview
2011 - 2011	Insights into the mechanism of molecular selectivity and recognition by carbohydrate-binding modules from Clostridium thermocellum Conference	9th Congress Carbohydrate Bioengeneering Meeting
2011 - 2011	NMR solution structure of the N-terminal Sequence (1-30) of ovine prion-loke (Doppel) protein Conference	3rd EU/CCPN NMR Conference
2010/07/04 - 2010/07/09	The Influence of Temperature and Binding on the Dynamics of CtCBM11 Conference	Joint EUROMAR 2010 and 17th ISMAR Conference
2008 - 2008	Structural Studies in Family 11 Carbohydrate-Binding Modules Congress	1st Portuguese-Spanish-British Joint Biophysics Congress
2008 - 2008	Molecular Determinants of Ligand Specificity in Family 11 Carbohydrate-Binding Modules (CBM11) Workshop	EMBO Practical course on X-ray crystal structure determination
2008 - 2008	Solution Structure of Hirsutellin A: an example of an economic ribotoxin Congress	IV Bienal del GERMN
2007 - 2007	The Solution Structure of Hirsutellin A; structure-function relationship of ribotoxins Congress	Euromar 2007
2006 - 2006	Molecular Determinants of Ligand Specificity in Family 11 Carbohydrate Binding Modules (CBM11): An X-ray Crystallography, NMR and Computational Chemistry Combined Approach Meeting	REQUIMTE-4º Encontro
2006 - 2006	Molecular Determinants of Ligand Specificity in Family 11 Carbohydrate Binding Modules (CBM11): An NMR, X-ray Crystallography and Computational Chemistry Combined Approach Congress	GERMN – III Reunião Bienal
2006 - 2006	Molecular Determinants of Ligand Specificity in Family 11 Carbohydrate-Binding Modules (CBM11) Meeting	XX Encontro Nacional da Sociedade Portuguesa de Química

Jury of academic degree

	Topic Role	Candidate name (Type of degree) Institution / Organization
2023/07/06	Discovery and Characterization of novel GlycO-selective mucinases (Thesis) Main arguer	Mariana Figueiredo Carreira (Other) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2023/07/05	Development of artificial compartments using peptides as model systems (Thesis) Main arguer	Inês Chibeles Antunes (Other) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2022/12/05	A molecular view on how a commensal bacterium thrives in the human gut (Thesis) Main arguer	Alícia Isabel Martins Candeias (Master) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2022/06/24	From 3D structure to function of a protein responsible for the formation of bacterial biofilms (Thesis) Main arguer	Leonor Torres (Other) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2022/06	Recognition of G-quadruplexes in microRNA precursors by nucleolin and its implications in cancer (Thesis) Arguer	Tiago André Afonso dos Santos (PhD) Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2022/01/31	Understanding the detailed mechanism of interaction between La-related proteins and their RNA targets (Thesis) Main arguer	Cláudio Alexandre Lopes Colaço (Master) King's College London, United Kingdom Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2021/12/10	NMR investigation of ion-pair modulation of protein structure and dynamics and their relation to protein stability (Thesis) Main arguer	Micael Simões Silva (PhD) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2021/07/14	Structural Characterization of GRP: A protein involved in the inhibition of mineral crystal formation in ectopic calcification (Thesis) Main arguer	Rita Ângelo (Other) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2021/06/26	Expressão, purificação e caracterização de mutantes do citocromo tri-hémico PpcA de Geobacter metallireducens (Thesis) Main arguer	Gonçalo Filipe Ferreira Damas (Other) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2019/11/27	Reconhecimento molecular por RMN da Lectina-galactose presente em Macrófagos (Thesis) Arguer	Rui Miguel Alegria Borralho (Master) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2019/11/15	Structural and Functional Characterization of a New Bacterial Target Against Tuberculosis: The Phosphatase PtpA (Thesis) Arguer	Andreia Fernandes (Master) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2018/06/19	Caracterização da interacção da endolisina Twort com o peptidoglicano de estafilococos aureus (Thesis) Arguer	Raquel Marques Cardoso (Other) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2018/05/22	Descriminação da função do domínio lectina da enzima GalNAc-T2 e GalNAc-T3 durante a glicosilação da mucina-1 (Thesis) Main arguer	Rodrigo Almeida Francisco (Other) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal

Association member

	Society Organization name	Role
2008/03 - Current	Rede Nacional de Ressonância Magnética Nuclear

Committee member

	Activity description Role	Institution / Organization
2020 - Current	Thesis Accompanying Committee Annual Assessment; PhD Program in Biotechnology; Student: Tomás Monteiro Fernandes Advisor / Consultant	Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2018/06 - 2018/06	1st Biophysics Party Member	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2012/05/15 - 2013/05/14	Management of national programs in all fields of science Other	Fundação para a Ciência e a Tecnologia, Portugal

Conference scientific committee

	Conference name	Conference host
2023/06/01 - 2033/06/01	3rd Meeting of Young Biophysicists	i3S, Porto

Course / Discipline taught

	Academic session	Degree Subject (Type)	Institution / Organization
2023 - Current	Bioquímica Estrutural		Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2020 - Current	Bioquímica Farmacêutica	Licenciatura em Bioquímica (Mestrado integrado)	Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2018/10/23 - 2019	PTNMR PhD Program	PTNMR PhD Program (Doutoramento)	Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2013 - 2013	Hands on course : NMR in protein interactions studies	Especialização (Curso médio)	Universidade Nova de Lisboa, Portugal
2012 - 2012	NMR: From Theory to Practice	Especialização (Curso médio)	Universidade da Beira Interior, Portugal
2011 - 2011	2nd Hands on course on ligand based NMR interaction studies	Especialização (Curso médio)	Universidade Nova de Lisboa, Portugal
2010 - 2010	Hands on course on ligand-based NMR interaction studies	Especialização (Curso médio)	Universidade Nova de Lisboa, Portugal
2010 - 2010	Nuclear Magnetic Resonance: From Theory to Practice	Extensão e divulgação científica (Curso médio)	Universidade Nova de Lisboa, Portugal
2009 - 2009	Nuclear Magnetic Resonance: From Theory to Practice	Especialização (Curso médio)	Universidade Nova de Lisboa, Portugal
2008 - 2008	Hands-on Course in Proteins and Proteomics	Especialização (Curso médio)	Universidade Nova de Lisboa, Portugal

Evaluation committee

	Activity description Role	Institution / Organization	Funding entity
2022/07/05 - Current	Early Career Researcher Award - ECRA Evaluator	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica

Interview (tv / radio show)

	Program	Topic
2022/01/29 - Current	Rádio Horizonte Algarve	DNAZymes

Distinctions

Award

2020	2020 Individual Call to Scientific Employment Stimulus Fundação para a Ciência e a Tecnologia, Portugal
2015	Marie Sklodowska-Curie Individual Fellowship European Commission, Portugal
2014	Humboldt Research Fellowship for Postdoctoral Researchers - Kindly declined Alexander von Humboldt-Foundation, Portugal
2013	Best Ph.D. Thesis in the field of NMR in 2012 António Xavier Award 2013, Portugal
2011	Five best poster award 9th Congress Carbohydrate Bioengineering Meeting, Portugal