Ana Maria Sequeira Cardoso (D113-0602-8986)

Curriculum Vitae

Ana Maria Sequeira Cardoso

Ana Maria Sequeira Cardoso concluded her PhD in Biosciences, specialization in Biochemistry, in 2014 from the University of Coimbra (Faculty of Sciences and Technology), her MSc in Biochemistry in 2009 from the University of Coimbra (Faculty of Sciences and Technology) and her BSc in Biochemistry in 2007 from the University of Coimbra (Faculty of Sciences and Technology). She is a researcher in CIBB (former CNC.IBILI), with 35 articles published in specialized journals, and four book chapters, summing 729 citations, which rendered her an h-index of 16 (Scopus). In 2010, Ana Cardoso received a PhD individual grant from the Portuguese Foundation for Science and Technology (FCT) to develop nucleic acid delivery systems, based on lipids, gemini surfactants, cell-penetrating peptides and thermoresponsive polymers, for gene therapy, to be conducted at Center for Neuroscience and Cell Biology, University of Coimbra (CNC). She studied the biophysical properties of nucleic acid delivery systems and established correlations between their biological activity, their structural characteristics and their interaction with biological or model membranes. She also addressed gene therapy directed towards mitochondria. In 2015, after her PhD, which she concluded in 2014 with honors, Ana Cardoso received an individual post-doctoral grant from FCT to study the molecular mechanisms underlying glioblastoma malignancy and to develop RNA-based strategies for glioblastoma therapy. From 2015 to 2019, still at CNC, she studied the role of non-protein coding RNAs in the regulation of energy metabolism in glioblastoma cells and evaluated the impact of gene therapy approaches targeting the expression of long non-coding RNAs and microRNAs involved in the metabolic phenotype of cancer cells, which is intimately connected with stem-like features displayed by tumors, glioblastoma cell invasion ability, and sensitivity to chemotherapeutic drugs. She also developed tridimensional in vitro models of glioblastoma cells (spheroids and organoids), that closely resemble the responses of glioblastoma tumors in their physiological environment, to test RNA-mediated therapeutic approaches. In 2019, Ana Cardoso was hired by CNC under the DL 57/2016 norm (current employment) and engaged in projects featuring the use of iPSCs and human cerebral organoids to study brain diseases with genetic etiology, such as frontotemporal dementia (FTD), and autism spectrum disorders (ASD). She characterized iPSCs reprogrammed from terminally differentiated somatic cells obtained from patients carrying mutations associated with the disease diagnostic, and produced human brain organoids following different protocols. These organoids are being used as platforms for the study of molecular, cellular and network features of neurodevelopment (ASD) and neurodegeneration (FTD). In parallel projects, Ana Cardoso studied miRNAs involved in the immune response in Alzheimer's Disease (AD) patients, including by performing peripheral blood mononuclear cell isolation, identification, determination of immune activity, chemotaxis and phagocytosis, and correlation with differential expression of immune-related microRNAs. She also helped establish a murine asthma model that was used to assess the epidemiological correlation between attention deficit and hyperactivity disorder (ADHD) with immune dysfunction in early childhood.

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Identification

Personal identification

Full name: Ana Maria Sequeira Cardoso

Citation names

Cardoso, A.M.

Author identifiers

Ciência ID: D113-0602-8986
ORCID iD: 0000-0002-1629-4782
Researcher Id: D-6563-2014
Scopus Author Id: 47961021900

Websites

https://cnc.uc.pt/en/research-group/neuronal-circuits-and-behavior (Professional)

Knowledge fields

Natural sciences - Biological Sciences - Cell Biology
Natural sciences - Biological Sciences - Biophysics
Medical and Health Sciences - Medical Biotechnology - Gene-based Diagnostics and Therapeutic Interventions
Medical and Health Sciences - Medical Biotechnology - Technologies Involving the Manipulation of Cells, Tissues, Organs or the Whole Organism

Languages

Language	Speaking	Reading	Writing	Listening
Portuguese (Mother tongue)
English	Proficiency (C2)	Proficiency (C2)	Proficiency (C2)	Proficiency (C2)
French	Beginner (A1)	Intermediate (B1)	Beginner (A1)	Beginner (A1)

Education

	Degree	Classification
2014 Concluded	Doutoramento em Biociências, especialização em Bioquímica (Doutoramento) Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal "Nucleic Acid Delivery Systems: From Biophysics to Biological Activity" (THESIS/DISSERTATION)	Aprovada com distinção e louvor por unanimidade
2009 Concluded	Mestrado em Bioquímica (2º ciclo de Bolonha) (Mestrado) Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal "Biophysical characterization of Gemini-based lipoplexes with different levels of biological activity" (THESIS/DISSERTATION)	18
2007 Concluded	Bioquímica (Licenciatura) Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal	14

Affiliation

Science

	Category Host institution	Employer
2020 - Current	Researcher (Research)	Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal

2019/01/15 - 2020	Researcher (Research)	CNC.IBILI, Portugal

Others

	Category Host institution	Employer
2015/01/02 - 2019/01/14	Post-Doc Researcher	CNC.IBILI, Portugal

2010/05/01 - 2014/12/23	Ph.D. student	CNC.IBILI, Portugal

2009/09/01 - 2010/04/30	Research Technician	CNC.IBILI, Portugal

Projects

Grant

	Designation	Funders
2015/01/02 - 2019/01/14	Targeting non-coding RNAs involved in the regulation of glycolysis/oxidative phosphorylation of glioblastoma cells towards tumor sensitization to chemotherapy SFRH/BPD/99613/2014 Post-doc Fellow CNC.IBILI, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2010/05/03 - 2014/04/30	Interaction of Lipoplexes with Cellular Membranes: a Biophysical Approach to Gene Delivery info:eu-repo/grantAgreement/FCT/5876-PPCDTI/103001/PT	Fundação para a Ciência e a Tecnologia Concluded

Contract

	Designation	Funders
2021/01/01 - 2025/12/31	Centro de Inovação em Biomedicina e Biotecnologia - Laboratório Associado LA/P/0058/2020 10.54499/LA/P/0058/2020 Researcher Universidade de Coimbra, Portugal Universidade Nova de Lisboa ¿Laboratório de Instrumentação Engenharia Biomédica e Física da Radiação, Portugal Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2022/09/01 - 2024/03/31	Center for Innovative Biomedicine and Biotechnology - Associate Laboratory LA/P/0058/2020 Universidade de Coimbra, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2017/01/01 - 2021/03/31	Estaminalidade das células do cancro: um desafio e uma oportunidade para avançar no tratamento em Oncologia SAICTPAC/0022/2015 Researcher Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal Instituto Nacional de Engenharia Biomédica, Portugal Fundação Calouste Gulbenkian, Portugal Universidade de Coimbra, Portugal Universidade do Porto Instituto de Patologia e Imunologia Molecular, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2019/01/01 - 2019/12/31	CNC. IBILI UID/NEU/04539/2019 Researcher Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal Universidade de Coimbra, Portugal Universidade de Coimbra Instituto de Ciências Nucleares Aplicadas à Saúde, Portugal Universidade de Coimbra Instituto de Investigação Clínica e Biomédica de Coimbra, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2011/01/01 - 2012/12/31	Strategic Project - LA 1 - 2011-2012 PEst-C/SAU/LA0001/2011 Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2009/09/01 - 2010/04/30	Development of a novel approach for anti-tumoral therapy based on the S4(13)-PV cell penetrating peptide to improve the intracellular delivery of therapeutic proteins and nucleic acids PTDC/BIO/65627/2006 Research Technician Fellow CNC.IBILI, Portugal	Fundação para a Ciência e a Tecnologia Concluded

Other

	Designation	Funders
2023/03/01 - 2026/02/28	Th2brain: Effects of Th2-related immunity in the postnatal maturation of brain circuits 2022.02604.PTDC Researcher Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2022/05/01 - 2025/04/30	Astrocytes dysfunctions in Phelan-McDermid syndrome: from mechanisms towards new therapeutic strategies 10.54499/NEURON/0002/2021 Co-Principal Investigator (Co-PI) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2021/01/01 - 2023/12/31	Disease modelling of frontotemporal dementia in human brain organoids PTDC/MEC-NEU/4814/2020 Researcher	Fundação para a Ciência e a Tecnologia
2019/01/01 - 2020/12/31	Projecto estratégico UID/NEU/04539/2019 UID/NEU/04539/2019 Researcher	Ongoing
2017/01/01 - 2020/12/31	BrainHealth2020 - Early Detection, Neuromodulation and Advanced Therapies to Brain Disorders CENTRO-01-0145-FEDER-000008 Researcher	Ongoing
2017/01/01 - 2019/12/31	Tackling CANcer STEM CELls: a challenge and an opportunity to advance in anti-cancer therapy POCI-01-0145-FEDER-016390 Researcher	Ongoing
2016/01/01 - 2018/12/31	A lipidomic and miRNA-based strategy for glioblastoma treatment A03/2016 Researcher	Concluded
2013/01/01 - 2015/12/31	Combined therapeutic approach based on miRNA modulation towards glioblastoma multiforme: A novel targeted lipidbased nanosystem for systemic delivery PTDC/DTP-FTO/0265/2012 Researcher	Concluded
2013/01/01 - 2014/12/31	Projecto estratégico CNC/UC: PEstC/SAU/LA0001/2013 – LA1 CNC/UC: PEstC/SAU/LA0001/2013 – LA1 Researcher	Concluded
2011/01/01 - 2012/12/31	Projecto estratégico CNC/UC: PEstC/SAU/LA0001/2011 - LA1 CNC/UC: PEstC/SAU/LA0001/2011 - LA1 Researcher CNC.IBILI, Portugal	Concluded

Outputs

Publications

Book chapter	Cardoso, Ana M.; Cardoso, Ana L; Pedroso de Lima, Maria C; Jurado, Amália S. "Non-viral therapeutic approaches for modulation of gene expression: nanotechnological strategies to overcome biological challenges". In Nanoparticles in the Life Sciences and Biomedicine, 409-432. Pan Stanford Publishing, 2018. Cardoso, Ana. "Gene delivery mediated by gemini surfactants: structure-activity relationships". In Engineering of Nanobiomaterials, 227-256. Elsevier, 2016. Published • 10.1016/B978-0-323-41532-3.00007-5 Cardoso, Ana. "Recent trends in nanotechnology towards CNS diseases: Lipid-based nanoparticles and exosomes for targeted therapeutic delivery". 2016. 10.1016/bs.irn.2016.05.002 Trabulo, S.; Cardoso, A.L.; Cardoso, A.M.S.; Düzgünes, N.; Jurado, A.S.; De Lima, M.C.P.. "Cell-penetrating peptide-based systems for nucleic acid delivery: A biological and biophysical approach". In Methods in Enzymology, 277-300. 2012. 10.1016/B978-0-12-391858-1.00014-9
Conference paper	Guedes, J. R.; Ferreira, P.A.; Soares-Cunha, Carina; Reis, T.; Almeida, J.S; LEBRE, CAROLINA; Costa, J.; et al. "Early-life IL-4 as a risk factor for Attention-Deficit/Hyperactivity Disorder". Paper presented in VIII Symposium Portuguese Glial Network, Braga, 2024.
Conference poster	Guedes J.R.; Ferreira P.; Costa J.; Laranjo, M.; Reis, T.; Cardoso, A.M.; et al.. "Allergies and IL-4 shape postnatal cerebellar development via microglia-mediated neuronal pruning to induce ADHD-like behaviors in mice". Paper presented in 9th FENS Forum 2022, 2022. Oliveira, A.R.; Cammarata, G.; Cardoso, A.M.; et al. "Autism associated CASPR2 auto-immune antibodies modify the developmental trajectory and network activity in human brain organoids". Paper presented in 9th FENS Forum 2022, 2022. Martins, S.; Martins, M.; Neves, M.; Cardoso, A.M.; et al. "Immunocompetent brain organoids and induced-microglia-like cells to study the pathological mechanisms of Frontotemporal Dementia". 2022. Neves, M.; Martins, S.; Oliveira, A.R.; Martins, M.; Cammarata, G.; Cardoso, A.M.; et al. "Generation and characterization of induced pluripotent stem cells derived from human dermal fibroblast lines of Frontotemporal Lobar Degeneration patients". 2022. Guedes, J. R.; Ferreira, P.A.; Costa, J.; Laranjo, M.; Reis, T.; Cardoso, A.M.; Casquinha, M.; et al. "Abnormal microglia-mediated pruning in the cerebellum induces circuitry and behavioral dysfunction in mice". Paper presented in XVII Meeting of the Portuguese Society for Neuroscience, 2021. Oliveira, A.R.; Seabra, C.M.; Guedes, J.R.; Cammarata, G.; Cardoso, A.M.; et al. "Uncovering the neurodevelopmental consequences of anti-CASPR2 antibody exposure in 3D brain organoids". 2021. Guedes, Joana; Ferreira, Pedro; Laranjo, Mariana; Reis, Tiago; Cardoso, A.M.; et al. "Abnormal microgia-mediated prunning in the cerebellum induces circuitry and behavioral disfunction in mice". Paper presented in XVII Meeting of the Portuguese Society for Neuroscience, 2021. Gomes, M; Pereira, M; Laranjo, M; Reis, T; Cardoso, A M; et al. "GPRASP2 (dys)function in metabolism and obesity". Paper presented in Regional Meeting of the International Society for Neurochemistry Meeting, 2021. Morais, Catarina; Cardoso, A.M.; at al.. "Silencing of key-enzymes of lipid metabolism combined with multitargeted receptor tyrosine kinase inhibitors as a new therapeutic strategy for glioblastoma". Paper presented in FEBS 2019 Advanced Course: Oncometabolism: From Conceptual Knowledge to Clinical Applications, 2019. Cardoso, A.M.; Sousa, Madalena G.; Morais, Catarina. "Combined therapeutic approach to tackle glioblastoma: modulation of miRNAs involved in bioenergetic metabolism for cell sensitization to chemotherapy". Paper presented in MitoPorto – Advances in Mitochondrial Research, 2017. Morais, Catarina; Cunha, Pedro; Cardoso, A.M.; et al. "Membrane perturbations induced by acylated S4(13)-PV analogs: relevance to nucleic acid delivery". Paper presented in 34th European Peptide Symposium, 2016. Cunha, Pedro; Costa, Raquel; Costa, Pedro; Cardoso, A.M.; et al. "A promising targeted strategy to overcome drug-resistance in glioblastoma". Paper presented in EMBO workshop: Modern DNA concepts and tools for safe gene transfer and modification, 2015. Cunha, Pedro; Costa, Pedro; Costa, Raquel; Custódia, Carlos; Branco, Ruben; Morais, Catarina; Cardoso, A.M.; et al. "MicroRNA modulation renders glioblastoma cells sensitive to chemotherapy". Paper presented in 49th Annual Scientific Meeting of the European Society for Clinical Investigation, 2015. Cruz, Rita; Morais, Catarina; Cardoso, A.M.; et al. "Gene silencing mediated by gemini-based formulations". Paper presented in XVIII Congress of the Portuguese Biochemical Society, 2014. Guedes, Joana; Santana, Isabel; Cunha, Catarina; Almeida, Rosário; Cardoso, A.M.; et al. "Molecular and phenotypic alterations in the mononuclear phagocyte system from Alzheimer’s disease patients". Paper presented in XVIII Congress of the Portuguese Biochemical Society, 2014. Cunha, Pedro; Costa, Pedro; Costa, Raquel; Branco, Ruben; Morais, Catarina; Cardoso, A.M.; et al. "MicroRNA-21 and micro RNA-128 modulation sensitizes glioblastoma cells to sunitinib treatement". Paper presented in XVIII Congress of the Portuguese Biochemical Society, 2014. Calvete, Mario; Pinto, Sara; Cardoso, A.M.; et al. "A biocompatible manganese (III) phthalocyanine for development of MRI contrast agents". Paper presented in COST Action TD1004, 2014. Morais, Catarina; Cardoso, A.M.; et al. "Nuclear and mitochondrial gene transfection mediated by cationic pentacyclic triterpenoid derivatives". Paper presented in GEM/GERLI Lipidomics Meeting: From Membranes to Pathologies, 2013. Cardoso, A.M.; et al. "The structural architecture of Bis-quat gemini surfactants is crucial for their DNA delivery ability". Paper presented in 9th EBSA – European Biophysics Congress, 2013. Silva, Sandra; Alves, Claudia; Cardoso, A.M.; et al. "Rational design of cleavable cationic gemini surfactants: exploring the multifunctionality of serine as headgroup". Paper presented in RICI5 – 5th Iberian Meeting on Colloids and Interfaces, 2013. Cardoso, A.M.; et al. "Gemini Surfactant-Based Gene Delivery Systems: Transfection Efficiency And Cytotoxicity Depend On Hydrocarbon Chain Length And Helper Lipids". Paper presented in III Congress of the Portuguese Society of Pharmaceutical Sciences/IX Spanish-Portuguese Conference on Controlled Drug Delivery - New Trends in Pharmacological Sciences, 2011. Cardoso, A.M.; Morais, Catarina; et al. "Transfection efficiency of Gemini surfactant-based gene delivery systems as affected by helper lipids". Paper presented in RICI4 - 4th Iberian Meeting on Colloids and Interfaces, 2011. Cardoso, A.M.; et al. "A biophysical study on the interaction of S4(13)-PV cell penetrating peptide with model lipid membranes". Paper presented in Meeting of Peptide Vectors and Delivery of Therapeutics, 2011. Cardoso, A.M.; et al. "A biophysical study on the interaction of S413-PV cell penetrating peptide with model lipid membranes". Paper presented in XVII Congresso Nacional de Bioquímica, 2010. Trabulo, Sara; Cardoso, A.M.; et al. "Silencing of survivin enhances the sensitivity of cancer cells to chemotherapeutic agents". Paper presented in 21st Meeting of the European Association for Cancer Research, 2010. Cardoso, A.M.; et al. "Biophysical properties and transfection ability of ethanediyl-alfa,omega-bis(-N-tetradecyl,N,N’,dimethylammonium bromide)-based vectors". Paper presented in International Courses on Toxicology, 2010.
Journal article	Guedes, Joana R.; Ferreira, Pedro A.; Costa, Jéssica; Laranjo, Mariana; Reis, Tiago; Cardoso, Ana Maria; Lebre, Carolina; et al. "IL-4 Shapes Microglia-Dependent Pruning of the Cerebellum During Postnatal Development". SSRN Electronic Journal (2023): http://dx.doi.org/10.2139/ssrn.4281069. Under revision • 10.2139/ssrn.4281069 Catarina M. Morais; Ana M. Cardoso; Ana Rita D. Araújo; Ana Reis; Pedro Domingues; Maria Rosário M. Domingues; Maria C. Pedroso de Lima; Amália S. Jurado. "Stearoyl CoA Desaturase-1 Silencing in Glioblastoma Cells: Phospholipid Remodeling and Cytotoxicity Enhanced upon Autophagy Inhibition". International Journal of Molecular Sciences (2022): https://doi.org/10.3390/ijms232113014. 10.3390/ijms232113014 Ana Rafaela Oliveira; Solange Martins; Giuseppe Cammarata; Mariana Martins; Ana Maria Cardoso; Maria Rosário Almeida; Maria do Carmo Macário; et al. "Generation and Characterization of Novel iPSC Lines from a Portuguese Family Bearing Heterozygous and Homozygous GRN Mutations". Biomedicines (2022): https://doi.org/10.3390/biomedicines10081905. 10.3390/biomedicines10081905 Regueira, Patrícia; Silva, Ana Rita; Cardoso, Ana Luísa; Cardoso, Ana Maria; et al.. "Peripheral inflammatory markers during an acute bacterial infection in older patients with and without cognitive dysfunction: a case control study". Brain Behavior and Immunity (2022): Submitted Cardoso, Ana M; Morais, Catarina M; Sousa, Madalena; Rebelo, Olinda; Tão, Hermínio; Barbosa, Marcos; Pedroso de Lima, Maria C; Jurado, Amália S. "MiR-200c-based metabolic modulation in glioblastoma cells as a strategy to overcome tumor chemoresistance". Human Molecular Genetics 30 23 (2021): 2315-2331. http://dx.doi.org/10.1093/hmg/ddab193. 10.1093/hmg/ddab193 Jéssica Costa; Solange Martins; Pedro A. Ferreira; Ana M.S. Cardoso; Joana R. Guedes; João Peça; Ana L. Cardoso. "The old guard: Age-related changes in microglia and their consequences". Mechanisms of Ageing and Development 197 (2021): 111512-111512. https://doi.org/10.1016/j.mad.2021.111512. 10.1016/j.mad.2021.111512 Cardoso, A.M.; Morais, C.M.; Rebelo, O.; Tão, H.; Barbosa, M.; Pedroso de Lima, M.C.; Jurado, A.S.. "Downregulation of long non-protein coding RNA MVIH impairs glioblastoma cell proliferation and invasion through a miR-302a-dependent mechanism". Human Molecular Genetics (2021): http://dx.doi.org/10.1093/hmg/ddab009. 10.1093/hmg/ddab009 Cardoso, A.M.; Morais, C.M.; Pena, F.; Marante, T.; Cunha, P.P.; Jurado, A.S.; Pedroso de Lima, M.C.. "Differentiation of glioblastoma stem cells promoted by miR-128 or miR-302a overexpression enhances senescence-associated cytotoxicity of axitinib". Human Molecular Genetics (2021): http://dx.doi.org/10.1093/hmg/ddab011. 10.1093/hmg/ddab011 Faria, Rúben; Albuquerque, Tânia; Neves, Ana R.; Bhatt, Himanshu; Biswas, Swati; Cardoso, Ana M.; Pedroso de Lima, Maria C.; Jurado, Amália S.; Costa, Diana. "Physicochemical characterization and targeting performance of triphenylphosphonium nano-polyplexes". Journal of Molecular Liquids 316 (2020): 113873. http://dx.doi.org/10.1016/j.molliq.2020.113873. 10.1016/j.molliq.2020.113873 Morais, Catarina M.; Cardoso, Ana M.; Aguiar, Luísa; Vale, Nuno; Nóbrega, Clévio; Zuzarte, Mónica; Gomes, Paula; Pedroso de Lima, Maria C.; Jurado, Amália S.. "Lauroylated Histidine-Enriched S413-PV Peptide as an Efficient Gene Silencing Mediator in Cancer Cells". Pharmaceutical Research 37 10 (2020): http://dx.doi.org/10.1007/s11095-020-02904-x. 10.1007/s11095-020-02904-x Silva, Francisco; Paulo, António; Pallier, Agnès; Même, Sandra; Tóth, Éva; Gano, Lurdes; Marques, Fernanda; et al. "Dual Imaging Gold Nanoplatforms for Targeted Radiotheranostics". Materials 13 3 (2020): 513. http://dx.doi.org/10.3390/ma13030513. Published • 10.3390/ma13030513 Barros-Viegas, Ana Teresa; Carmona, Vítor; Ferreiro, Elisabete; Guedes, Joana; Cardoso, Ana Maria; Cunha, Pedro; Pereira de Almeida, Luís; et al. "MiRNA-31 improves cognition and abolishes amyloid-ß pathology by targeting APP and BACE1 in an animal model of Alzheimer’s disease". Molecular Therapy - Nucleic Acids (2020): http://dx.doi.org/10.1016/j.omtn.2020.01.010. Accepted • 10.1016/j.omtn.2020.01.010 Catarina M Morais; Pedro P Cunha; Tânia Melo; Ana M Cardoso; Pedro Domingues; M Rosário Domingues; Maria C Pedroso de Lima; Amália S Jurado. "Glucosylceramide synthase silencing combined with the receptor tyrosine kinase inhibitor axitinib as a new multimodal strategy for glioblastoma". Human Molecular Genetics (2019): https://doi.org/10.1093/hmg/ddz152. 10.1093/hmg/ddz152 Ana M S Cardoso; Madalena Sousa; Catarina M Morais; Liliana R Oancea-Castillo; Anne Régnier-Vigouroux; Olinda Rebelo; Hermínio Tão; et al. "MiR-144 overexpression as a promising therapeutic strategy to overcome glioblastoma cell invasiveness and resistance to chemotherapy". Human Molecular Genetics (2019): https://doi.org/10.1093/hmg/ddz099. 10.1093/hmg/ddz099 Pinto, Sara M. A.; Calvete, Mário J. F.; Ghica, Mariana E.; Soler, Sérgio; Gallardo, Iluminada; Pallier, Agnès; Laranjo, Mariana B.; et al. "A biocompatible redox MRI probe based on a Mn(ii)/Mn(iii) porphyrin". Dalton Transactions 48 10 (2019): 3249-3262. http://dx.doi.org/10.1039/c8dt04775h. 10.1039/c8dt04775h Morais, Catarina M.; Cardoso, Ana M.; Cunha, Pedro P.; Aguiar, Luísa; Vale, Nuno; Lage, Emílio; Pinheiro, Marina; et al. "Acylation of the S413-PV cell-penetrating peptide as a means of enhancing its capacity to mediate nucleic acid delivery: Relevance of peptide/lipid interactions". Biochimica et Biophysica Acta (BBA) - Biomembranes 1860 12 (2018): 2619-2634. http://dx.doi.org/10.1016/j.bbamem.2018.10.002. 10.1016/j.bbamem.2018.10.002 Viegas, A.T.B.; Guedes, J.R.; Oliveira, A.R.; Cardoso, A.M.S.; Cardoso, A.L.C.. "miRNAs: New biomarkers and therapeutic targets in dementia". Current Pharmaceutical Design 23 5 (2017): 669-692. http://www.scopus.com/inward/record.url?eid=2-s2.0-85017530528&partnerID=MN8TOARS. 10.2174/1381612823666170111094702 Cunha, P.P.; Costa, P.M.; Morais, C.M.; Lopes, I.R.; Cardoso, A.M.; Cardoso, A.L.; Mano, M.; Jurado, A.S.; Pedroso De Lima, M.C.P.. "High-throughput screening uncovers miRNAs enhancing glioblastoma cell susceptibility to tyrosine kinase inhibitors". Human Molecular Genetics 26 22 (2017): 4375-4387. http://www.scopus.com/inward/record.url?eid=2-s2.0-85034256071&partnerID=MN8TOARS. 10.1093/hmg/ddx323 Cruz, R.Q.; Morais, C.M.; Cardoso, A.M.; Silva, S.G.; Vale, M.L.; Marques, E.F.; Pedroso De Lima, M.C.; Jurado, A.S.. "Enhancing glioblastoma cell sensitivity to chemotherapeutics: A strategy involving survivin gene silencing mediated by gemini surfactant-based complexes". European Journal of Pharmaceutics and Biopharmaceutics 104 (2016): 7-18. http://www.scopus.com/inward/record.url?eid=2-s2.0-84964330230&partnerID=MN8TOARS. 10.1016/j.ejpb.2016.04.014 Pinto, S.M.A.; Tomé, V.A.; Calvete, M.J.F.; Pereira, M.M.; Burrows, H.D.; Cardoso, A.M.S.; Pallier, A.; et al. "The quest for biocompatible phthalocyanines for molecular imaging: Photophysics, relaxometry and cytotoxicity studies". Journal of Inorganic Biochemistry 154 (2016): 50-59. http://www.scopus.com/inward/record.url?eid=2-s2.0-84946887800&partnerID=MN8TOARS. 10.1016/j.jinorgbio.2015.10.013 Guedes, J.R.; Santana, I.; Cunha, C.; Duro, D.; Almeida, M.R.; Cardoso, A.M.; de Lima, M.C.P.; Cardoso, A.L.. "MicroRNA deregulation and chemotaxis and phagocytosis impairment in Alzheimer's disease". Alzheimer's and Dementia: Diagnosis, Assessment and Disease Monitoring 3 (2016): 7-17. http://www.scopus.com/inward/record.url?eid=2-s2.0-84956620147&partnerID=MN8TOARS. 10.1016/j.dadm.2015.11.004 Cardoso, A.M.; Morais, C.M.; Cruz, A.R.; Silva, S.G.; Do Vale, M.L.; Marques, E.F.; De Lima, M.C.P.; Jurado, A.S.. "New serine-derived gemini surfactants as gene delivery systems". European Journal of Pharmaceutics and Biopharmaceutics 89 (2015): 347-356. http://www.scopus.com/inward/record.url?eid=2-s2.0-84920830778&partnerID=MN8TOARS. 10.1016/j.ejpb.2014.12.013 Cardoso, A.M.; Morais, C.M.; Cruz, A.R.; Cardoso, A.L.; Silva, S.G.; Do Vale, M.L.; Marques, E.F.; Pedroso De Lima, M.C.; Jurado, A.S.. "Gemini Surfactants Mediate Efficient Mitochondrial Gene Delivery and Expression". Molecular Pharmaceutics 12 3 (2015): 716-730. http://www.scopus.com/inward/record.url?eid=2-s2.0-84924023696&partnerID=MN8TOARS. 10.1021/mp5005349 Cardoso, A.M.; Calejo, M.T.; Morais, C.M.; Cardoso, A.L.; Cruz, R.; Zhu, K.; Pedroso De Lima, M.C.; Jurado, A.S.; Nyström, B.. "Application of thermoresponsive PNIPAAM-b-PAMPTMA diblock copolymers in siRNA delivery". Molecular Pharmaceutics 11 3 (2014): 819-827. http://www.scopus.com/inward/record.url?eid=2-s2.0-84901742907&partnerID=MN8TOARS. 10.1021/mp400510a Kjøniksen, A.-L.; Calejo, M.T.; Zhu, K.; Cardoso, A.M.S.; De Lima, M.C.P.; Jurado, A.S.; Nyström, B.; Sande, S.A.. "Sustained release of naltrexone from Poly(N-Isopropylacrylamide) microgels". Journal of Pharmaceutical Sciences 103 1 (2014): 227-234. http://www.scopus.com/inward/record.url?eid=2-s2.0-84890551451&partnerID=MN8TOARS. 10.1002/jps.23780 Cardoso, A.M.; Morais, C.M.; Silva, S.G.; Marques, E.F.; De Lima, M.C.P.; Jurado, M.A.S.. "Bis-quaternary gemini surfactants as components of nonviral gene delivery systems: A comprehensive study from physicochemical properties to membrane interactions". International Journal of Pharmaceutics 474 1-2 (2014): 57-69. http://www.scopus.com/inward/record.url?eid=2-s2.0-84906267891&partnerID=MN8TOARS. 10.1016/j.ijpharm.2014.08.011 Calejo, M.T.; Cardoso, A.M.S.; Kjøniksen, A.-L.; Zhu, K.; Morais, C.M.; Sande, S.A.; Cardoso, A.L.; et al. "Temperature-responsive cationic block copolymers as nanocarriers for gene delivery". International Journal of Pharmaceutics 448 1 (2013): 105-114. http://www.scopus.com/inward/record.url?eid=2-s2.0-84875930565&partnerID=MN8TOARS. 10.1016/j.ijpharm.2013.03.028 Trabulo, S.; Cardoso, A.L.; Cardoso, A.M.S.; Morais, C.M.; Jurado, A.S.; Pedroso de Lima, M.C.. "Cell-penetrating peptides as nucleic acid delivery systems: From biophysics to biological applications". Current Pharmaceutical Design 19 16 (2013): 2895-2923. http://www.scopus.com/inward/record.url?eid=2-s2.0-84878655380&partnerID=MN8TOARS. 10.2174/1381612811319160006 Calejo, M.T.; Cardoso, A.M.S.; Marques, E.F.; Araújo, M.J.; Kjøniksen, A.-L.; Sande, S.A.; de Lima, M.C.P.; Jurado, A.S.; Nyström, B.. "In vitro cytotoxicity of a thermoresponsive gel system combining ethyl(hydroxyethyl) cellulose and lysine-based surfactants". Colloids and Surfaces B: Biointerfaces 102 (2013): 682-686. http://www.scopus.com/inward/record.url?eid=2-s2.0-84867835593&partnerID=MN8TOARS. 10.1016/j.colsurfb.2012.09.033 Silva, S.G.; Alves, C.; Cardoso, A.M.S.; Jurado, A.S.; Pedroso De Lima, M.C.; Vale, M.L.C.; Marques, E.F.. "Synthesis of gemini surfactants and evaluation of their interfacial and cytotoxic properties: Exploring the multifunctionality of serine as headgroup". European Journal of Organic Chemistry 9 (2013): 1758-1769. http://www.scopus.com/inward/record.url?eid=2-s2.0-84875008251&partnerID=MN8TOARS. 10.1002/ejoc.201201396 Cardoso, A.M.; Trabulo, S.; Cardoso, A.L.; Maia, S.; Gomes, P.; Jurado, A.S.; Pedroso De Lima, M.C.. "Comparison of the efficiency of complexes based on S413-PV cell-penetrating peptides in plasmid DNA and siRNA delivery". Molecular Pharmaceutics 10 7 (2013): 2653-2666. http://www.scopus.com/inward/record.url?eid=2-s2.0-84879735661&partnerID=MN8TOARS. 10.1021/mp400078h Cardoso, A.M.S.; Trabulo, S.; Cardoso, A.L.; Lorents, A.; Morais, C.M.; Gomes, P.; Nunes, C.; et al. "S4(13)-PV cell-penetrating peptide induces physical and morphological changes in membrane-mimetic lipid systems and cell membranes: Implications for cell internalization". Biochimica et Biophysica Acta - Biomembranes 1818 3 (2012): 877-888. http://www.scopus.com/inward/record.url?eid=2-s2.0-84856059612&partnerID=MN8TOARS. 10.1016/j.bbamem.2011.12.022 Calejo, M.T.; Kjoniksen, A.-L.; Pinazo, A.; Pérez, L.; Cardoso, A.M.S.; Pedroso De Lima, M.C.; Jurado, A.S.; Sande, S.A.; Nyström, B.. "Thermoresponsive hydrogels with low toxicity from mixtures of ethyl(hydroxyethyl) cellulose and arginine-based surfactants". International Journal of Pharmaceutics 436 1-2 (2012): 454-462. http://www.scopus.com/inward/record.url?eid=2-s2.0-84865288628&partnerID=MN8TOARS. 10.1016/j.ijpharm.2012.07.018 Cardoso, A.M.S.; Faneca, H.; Almeida, J.A.S.; Pais, A.A.C.C.; Marques, E.F.; De Lima, M.C.P.; Jurado, A.S.. "Gemini surfactant dimethylene-1,2-bis(tetradecyldimethylammonium bromide)-based gene vectors: A biophysical approach to transfection efficiency". Biochimica et Biophysica Acta - Biomembranes 1808 1 (2011): 341-351. http://www.scopus.com/inward/record.url?eid=2-s2.0-78649777308&partnerID=MN8TOARS. 10.1016/j.bbamem.2010.09.026 Trabulo, S.; Cardoso, A.M.; Santos-Ferreira, T.; Cardoso, A.L.; Simões, S.; Pedroso De Lima, M.C.. "Survivin silencing as a promising strategy to enhance the sensitivity of cancer cells to chemotherapeutic agents". Molecular Pharmaceutics 8 4 (2011): 1120-1131. http://www.scopus.com/inward/record.url?eid=2-s2.0-79961082146&partnerID=MN8TOARS. 10.1021/mp100426e

Activities

Oral presentation

	Presentation title	Event name Host (Event location)
2021	Multiparametric MRI characterization of the G144 GBM model	38th Annual Scientific Meeting of the European Society for Magnetic resonance in Medicine and Biology (ESMRMB) (Barcelona, Spain)
2019	Long Non-Protein Coding RNA MVIH Silencing as a Therapeutic Approach for Glioblastoma	3rd International Symposium on Frontiers in Molecular Science - RNA Regulatory Networks (Lisboa, Portugal)
2019	Silencing of key-enzymes of lipid metabolism combined with multitargeted receptor tyrosine kinase inhibitors as a new therapeutic strategy for glioblastoma	II FEBS Advanced Lecture Course on Oncometabolism-From Conceptual Knowledge to Clinical Applications (Luso, Portugal)
2019	Upgrading S413-PV peptide to mediate gene silencing in cancer cells through peptide lauroylation and histidine-enrichment	III International Congress in Health Sciences Research: Towards Innovation and Entrepreneurship - Trends in Aging and Cancer (Covilhã, Portugal)
2018	Gold Nanoparticles as Multimodal Imaging Agents	XIX Brazilian Meeting on Inorganic Chemistry, VI Latin American Meeting on Biological Inorganic Chemistry, VIII Brazilian Meeting on Rare Earths (Fortaleza, Brazil)
2017	Membrane lipids: protein partners for good and for evil. A case study in glioblastoma	6th Workshop in Lipidomics (Aveiro, Portugal)
2016	Interaction of acylated S413-PV analogs with lipid membranes	15th Iberian Peptide Meeting (Porto, Portugal)
2014	The interaction of gene delivery systems with membranes as a modulator of transfection efficiency	3rd Workshop in Lipidomics (Aveiro, Portugal)
2014	Improving the ability of S4(13)PV cell-penetrating peptide to mediate siRNA delivery through peptide acylation	SFNano In vitro/in vivo correlation workshop – Relevant tools and models for the translation of advanced drug delivery systems (Porto, Portugal)
2014	Gemini surfactants as gene delivery systems: disclosing the properties leading to transfection activity	20th International Symposium on Surfactants in Solution (Coimbra, Portugal)
2013	Gene Transfer Mediated by Bis-Quaternary Gemini Surfactants Depends on Complex Architecture	RICI5 – 5th Iberian Meeting on Colloids and Interfaces (San Sebastian, Spain)
2013	Efficient siRNA delivery mediated by S4(13)-PV cell penetrating peptide-based systems: a comparative study	13th Naples Workshop on Bioactive Peptides – Conformation and Activity in Peptides: Relationships and Interactions (Nápoles, Italy)
2012	Towards a biocompatible thermo-responsive hydrogel for pharmaceutical applications – The combined system of ethyl(hydroxyethyl) cellulose and lysine-based surfactants	CRS Nordic Chapter - Drug Delivery and Targeting (Reykjavik, Iceland)
2011	Membrane lipid interactions with cell-penetrating peptides: lessons on the role of lipids in membrane functioning	RICI4 - 4th Iberian Meeting on Colloids and Interfaces (Porto, Portugal)
2011	Membrane lipid interactions with cell-penetrating peptides: lessons on the role of lipids in membrane functioning	RICI4 - 4th Iberian Meeting on Colloids and Interfaces (Porto, Portugal)
2011	Transfection efficiency of Gemini surfactant-based gene delivery systems as affected by nature of formulation and method of preparation	XXXVI Jornadas Portuguesas de Genética (Coimbra, Portugal)

Supervision

	Thesis Title Role	Degree Subject (Type) Institution / Organization
2022/01/01 - Current	Biomarkers to stratify patients and predict outcomes in brain-associated diseases, from bench to bedside Co-supervisor	Molecular and Cell Biology Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2022/09 - 2023/09	The Hidden Player: Non-canonical role of Shank3 in neurodevelopment Supervisor	Universidade de Coimbra Departamento de Ciências da Vida, Portugal
2023/01 - 2023/08	The Role of Shank3 Mutation in Primary Cilia Length and Function during Neural Development Co-supervisor	Vrije Universiteit Amsterdam, Netherlands
2015/09/01 - 2016/09/10	MiRNA-based metabolic modulation in glioblastoma cells: a strategy to surpass tumor chemoresistance Supervisor	CNC.IBILI, Portugal

Event participation

	Activity description Type of event	Event name Institution / Organization
2023/05 - Current	Science Communication for pre-school kids Round table	Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2017/10 - 2017/11	Nature Masterclasses Online Course: Focus on Peer Review Other	Focus on Peer Review Springer Nature, Luxembourg
2017/01 - 2017/02	Coursera Online Course: Design and Interpretation of Clinical Trials Other	Design and Interpretation of Clinical Trials Johns Hopkins University Center for Global Health, United States
2016/10 - 2016/11	Coursera Online Course: Basics of Extracellular Vesicles Other	Basics of Extracellular Vesicles University of California Irvine Cancer Research Institute, United States
2016/03 - 2016/05	Coursera Online Course: Epigenetic Control of Gene Expression Other	Epigenetic Control of Gene Expression The University of Melbourne, Australia
2015/06 - 2015/06	Course: MiRNAs and non-coding RNAs in cell biology and human disease Other	MiRNAs and non-coding RNAs in cell biology and human disease Instituto de Medicina Molecular, Portugal
2013/09 - 2013/09	Course in Laboratorial Animal Science, category C of FELASA Other	Course in Laboratorial Animal Science Universidade de Coimbra, Portugal
2012/06 - 2012/06	Course of Science Communication for Researchers Other	Course of Science Communication for Researchers Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal

Jury of academic degree

	Topic Role	Candidate name (Type of degree) Institution / Organization
2023/10/20	Immunotolerance Strategies to Improve Cell-Based Therapy Outcomes in MJD (Thesis) Main arguer	Luís Carlos Oliveira (Master) Universidade de Coimbra Faculdade de Farmácia, Portugal
2022/10/28	Characterization of MJD patient's IPSC-derived NESC silenced for mutant ATAXIN-3 (Thesis) Main arguer	Mariana Machado (Master) Universidade de Coimbra Faculdade de Medicina, Portugal
2016/07/07	Avaliação do efeito da mutação c.900-901dupGT do gene GRN na resposta imune e autofagia: um estudo em portadores heterozigóticos e homozigóticos (Thesis) Main arguer	Catarina Isabel Carvalhas de Almeida (Other) Instituto Politécnico de Coimbra Escola Superior Agrária de Coimbra, Portugal

Ad Hoc journal article review

	Journal title (ISSN)	Publisher
2021 - Current	Cellular and Molecular Life Sciences
2020 - Current	Cancers
2019 - Current	Computational Biology and Chemistry
2019 - Current	Current Drug Metabolism
2019 - Current	Gene
2019 - Current	Journal of Cellular and Molecular Medicine
2019 - Current	Journal of Molecular Liquids
2016 - Current	International Journal of Nanomedicine
2015 - Current	Biomaterials
2015 - Current	Colloids and Surfaces B
2015 - Current	Molecular Pharmaceutics Journal
2014 - Current	Colloids and Surfaces A
2014 - Current	Journal of Applied Solution Chemistry and Modeling

Course / Discipline taught

	Academic session	Degree Subject (Type)	Institution / Organization
2021 - 2022	Electrophysiology of plasma membranes: Patch clamp	CELL CULTURE AND TISSUE TRAINING COURSE (Outros)	Universidade de Coimbra Faculdade de Medicina, Portugal
2018 - 2019	LabTalks	Exploring the role of circulating miRNAs in Delirium and Dementia (Mestrado)	Universidade de Coimbra Departamento de Ciências da Vida, Portugal
2018 - 2019	LabTalks	Targeted gene/drug therapies for brain tumors: from the immune microenvironment to the stem cell niche (Mestrado)	Universidade de Coimbra Departamento de Ciências da Vida, Portugal
2016 - 2019	Lnc-RNAs for cancer therapy	Advanced Therapies (Curso de doutoramento (conclusão de unidades curriculares))	Universidade de Coimbra Instituto de Investigação Interdisciplinar, Portugal
2013 - 2014	Lipidómica Funcional	MSc in Biochemistry (Mestrado)	Universidade de Coimbra Departamento de Ciências da Vida, Portugal
2011 - 2014	Toxicologia Bioquímica e Biofísica	Bioquímica (Mestrado)	Universidade de Coimbra Departamento de Ciências da Vida, Portugal
2011 - 2012	Biomembranas	MSc in Biochemistry (Mestrado)	Universidade de Coimbra Departamento de Ciências da Vida, Portugal

Mentoring / Tutoring

	Topic	Student name
2021 - 2022	The Phelan McDermid Syndrome main culprit: a short monograph about SHANK3	Mariana Cardoso Ribeiro
2021 - 2022	Medições do Estado Bioelétrico de Células Cancerígenas e Sua Manipulação	Catarina Bazenga Baptista Jardim
2021 - 2022	Medições do estado bioelétrico de células U87 do glioblastoma humano e sua manipulação	Daniela C. L. Silva
2019 - 2021	Extracellular vesicles and their role in the tumor microenvironment of glioblastoma multiforme	Guilherme Faria
2019 - 2020	Isolation and characterization of extracellular vesicles produced from glioblastoma cells and assessment of the impact of temozolomide in glioblastoma spheroid invasion	Inês Oliveira
2019 - 2020	Modelos celulares in vitro para o estudo de terapias para glioblastoma: Vantagens e desvantagens	Susana Gomes
2018 - 2019	Isolamento e caracterização de exossomas de células de glioblastoma	Francisco Tiago
2018 - 2019	Validação de um protocolo de fracionamento celular para quantificação de proteína em diferentes compartimentos celulares	Inês Oliveira
2014 - 2015	Modulação dos níveis de miRNAs envolvidos em alterações metabólicas em linhas celulares de glioblastoma (U87 e DBTRG) como abordagem terapêutica	Catarina Luz
2014 - 2015	Estratégia terapêutica para glioblastoma baseada no silenciamento de um gene do metabolismo lipídico combinado com a utilização de um fármaco quimioterapêutico	Márcia Teixeira
2012 - 2013	Surfactantes Gemini Catiónicos no Transporte de Material Genético: Abordagem Biológica e Biofísica	Ana Rita Cruz

Distinctions

Other distinction

2015	Bolsa Individual de Pos-Doutoramento Fundação para a Ciência e a Tecnologia, Portugal
2010	Bolsa Individual de Doutoramento Fundação para a Ciência e a Tecnologia, Portugal