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João Paulo Castro de Sousa. Concluiu o(a) Doutoramento em Microcirurgia Ocular (Cirurgia Vítreo-Retina / Microscopia Eletrónica / Histologia) em 2006 pelo(a) Universitat Autònoma de Barcelona, Mestrado em Ciências da Visão (Retinopatia Diabética) em 1996 pelo(a) Universidade de Coimbra Faculdade de Medicina e Licenciatura em Medicina em 1986 pelo(a) Universidade de Coimbra Faculdade de Medicina. É Senior Hospital Assistant of Ophthalmology / Vitreoretinal Surgeon. Director of Ophthalmology Service (Currently) no(a) Centro Hospitalar Leiria EPE, Assistant Professor of Ophthalmology. no(a) Universidade da Beira Interior Faculdade de Ciências da Saúde e Investigador no(a) Universidade da Beira Interior Centro de Investigação em Ciências da Saúde. Publicou 24 artigos em revistas especializadas. Recebeu 1 prémio(s) e/ou homenagens. Participa e/ou participou como Investigador em 3 projeto(s).
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Identification

Personal identification

Full name
João Paulo Castro de Sousa

Citation names

  • João Paulo Castro de Sousa

Author identifiers

Ciência ID
D81B-D3DE-23D2
ORCID iD
0000-0002-0025-1841

Languages

Language Speaking Reading Writing Listening Peer-review
Spanish; Castilian Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Education
Degree Classification
2000 - 2006
Concluded
 Microcirurgia Ocular (Cirurgia Vítreo-Retina / Microscopia Eletrónica / Histologia) (Doutoramento)
Universitat Autònoma de Barcelona, Spain
"n/a" (THESIS/DISSERTATION)
 n/a
1994 - 1996
Concluded
 Ciências da Visão (Retinopatia Diabética) (Mestrado)
Universidade de Coimbra Faculdade de Medicina, Portugal
"n/a" (THESIS/DISSERTATION)
 n/a
1989 - 1994
Concluded
 Oftalmologia (Especialização pós-licenciatura)
Centro Hospitalar e Universitario de Coimbra EPE Campus dos Hospitais da Universidade de Coimbra, Portugal
 n/a
1980 - 1986
Concluded
 Medicina (Licenciatura)
Universidade de Coimbra Faculdade de Medicina, Portugal
"n/a" (THESIS/DISSERTATION)
 n/a
Affiliation

Science

Category
Host institution 		Employer
2017 - Current Researcher (Research) Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2002 - 2006 Researcher (Research) Instituto de Microcirugía Ocular, Spain
Instituto de Microcirugía Ocular, Spain
1992 - 1996 Researcher (Research) CNC.IBILI, Portugal
CNC.IBILI, Portugal
(...)
1994 - 1994 Visiting Researcher (Research) Uniklinik Köln Klinik I für Innere Medizin, Germany
Uniklinik Köln Klinik I für Innere Medizin, Germany

Others

Category
Host institution 		Employer
2014 - Current Senior Hospital Assistant of Ophthalmology / Vitreoretinal Surgeon. Director of Ophthalmology Service (Currently) Centro Hospitalar Leiria EPE, Portugal
2006/09/01 - Current Assistant Professor of Ophthalmology. Universidade da Beira Interior Faculdade de Ciências da Saúde, Portugal
1991/09/01 - 1994/07/01 Unidade Curricular de Oftalmologia com Prof. Dr. Cunha-Vaz. Universidade de Coimbra Faculdade de Medicina, Portugal
1991/09/01 - 1992/07/01 Docente responsável pelo curso de Curso de Ortóptica. Instituto Politécnico de Coimbra Escola Superior de Tecnologia da Saúde de Coimbra, Portugal
Projects

Contract

Designation Funders
2021/10/01 - 2022/10/01 Projeto COMBINE “CombinatiOn aptaMer-drug Based therapeutics for ocular neovascular dIsease: wheN two are bettEr than one”
.
Researcher
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
 Ordem dos Farmaceuticos
Concluded
2015/11/01 - 2019/10/31 Vitreous humor biomarkers in Age-related Macular Degeneration
SFRH/BD/112526/2015
 Fundação para a Ciência e a Tecnologia
Concluded
2014 - 2015 Proteomics of the vitreous humor of patients with retinal pathologies
Protocolo UBI-Novartis Farma - Produtos Farmacêuticos, SA
Researcher
Novartis Farma SA
Concluded
2012 - 2013 Proteomics of the vitreous humor of patients with retinal pathologies
Protocolo UBI-Novartis Farma, Produtos Farmacêuticos, SA
Researcher
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
 Novartis Farma SA
Concluded
Outputs

Publications

Conference paper
  1. Fátima Milhano dos Santos; Gaspar, Leonor M.; Albuquerque, Tânia Gonçalves; João ML Dias; João Paulo Castro de Sousa; Alberto Paradela; Tomaz, Cândida; Passarinha, Luís. "Improvement of bidimensional electrophoretic conditions for vitreous protein analysis using an artificial neural network.". Paper presented in 12th European Symposium on Biochemical Engineering Science, 2018.
  2. Joana Mesquita; João Paulo Castro de Sousa; Sara Vaz-Pereira; Arminda Neves; Passarinha, Luís; Tomaz, Cândida. "Quantitative research of VEGF-A, VEGF-B and PIGF in vitreous and serum in angiogenic ocular disorders.". Paper presented in ARVO Annual Meeting, 2018.
    Published
  3. Joana Mesquita; João Paulo Castro de Sousa; Sara Vaz-Pereira; Arminda Neves; Paulo Tavares-Ratado; Passarinha, Luís; Tomaz, Cândida. "Quantitative analysis and correlation of VEGF-A and VEGF-B in serum and vitreous humor of patients with proliferative vs. non-proliferative ocular disease.". Paper presented in ARVO Annual Meeting, 2017.
    Published
  4. Joana Mesquita; João Paulo Castro de Sousa; Sara Vaz-Pereira; Arminda Neves; Paulo Tavares-Ratado; Passarinha, Luís; Tomaz, Cândida. "Correlation between concentration of vitreous angiogenic cytokines (VEGF-B and PIGF) with central retinal thickness and macular volume in diabetic retinopathy patients". Paper presented in ARVO Annual Meeting, 2016.
    Published
  5. Joana Mesquita; Sousa, João; Paulo Tavares Ratado; Sara Vaz-Pereira; Arminda Neves; Ana Sílvia Rocha; Fátima Milhano dos Santos; Passarinha, Luís; Tomaz, C. T.. "Comparison of serum and vitreous PIGF in diabetic retinopathy patients and non-diabetic patients". Paper presented in ARVO Annual Meeting, 2015.
  6. Joana Mesquita; Sousa, João; Ana Sílvia Rocha; Fátima Milhano dos Santos; João Monteiro; Luís A. Passarinha; Cândida T. Tomaz. "Quantification and comparison of VEGF-B in the vitreous of patients with diabetic ocular disease and a control group of patients with non-diabetic ocular disease". Paper presented in ARVO Annual Meeting, 2014.
    Published
Conference poster
  1. Fátima Milhano dos Santos; Sergio Ciordia; Alberto Paradela; Sousa, João; Marta Garcia Flores; Carla Cruz; Tomaz, Cândida; Passarinha, Luís. "Label-Free RelativeQuantification For Vitreous Proteome Profiling In Age-Related MacularDegeneration And Diabetic Retinopathy". Paper presented in III InternationalCongress in Health Sciences Research towards innovation and entrepreneurship, 2019.
  2. Fátima Milhano dos Santos; Gaspar, Leonor M.; Sergio Ciordia; Sousa, João; Alberto Paradela; Passarinha, Luís; Tomaz, Cândida. "Characterization of proteome of vitreous humor in retinal detachment using different experimental setups.". Paper presented in 16th Human Proteome Organisation World Congress, 2017.
  3. Fátima Milhano dos Santos; Gaspar, Leonor M.; Sergio Ciordia; João Paulo Castro de Sousa; Alberto Paradela; Passarinha, Luís; Tomaz, Cândida. "Proteome of vitreous humor in retinal detachment.". Paper presented in XII Annual CICS Symposium, 2017.
  4. Gaspar, Leonor M.; Fátima Milhano dos Santos; Albuquerque, Tânia Gonçalves; João Paulo Castro de Sousa; Passarinha, Luís; Tomaz, Cândida. "Proteomic analysis of vitreous humor samples in retinal detachment.". Paper presented in 5th Cycle of Conferences of the Faculty of Sciences, 2017.
  5. Fátima Milhano dos Santos; Gaspar, Leonor M.; Sergio Ciordia; Sousa, João; Alberto Paradela; Passarinha, Luís; Tomaz, Cândida. "ITRAQ quantitative analysis of vitreous in Proliferative Diabetic Retinopathy.". Paper presented in Dia da Ciência, 2017.
  6. Fátima Milhano dos Santos; Gaspar, Leonor M.; Albuquerque, Tânia; João ML Dias; Sousa, João; Alberto Paradela; Tomaz, Cândida; Passarinha, Luís. "Enhancement of bidimensionalelectrophoretic operation conditions by an artificial neural network for vitreous humor protein analysis". Paper presented in International Congress onAnalytical Proteomics, 2017.
  7. Gaspar, Leonor M.; Fátima Milhano dos Santos; Sousa, João; Passarinha, Luís; Tomaz, Cândida. "Proteomic analysis of the human vitreous humor in retinal detachment". Paper presented in IV International Conference on Analytical Proteomics, 2015.
  8. Fátima Milhano dos Santos; Gaspar, Leonor M.; Ana Sílvia Rocha; Sergio Ciordia; Sousa, João; Alberto Paradela; Passarinha, Luís; Tomaz, Cândida. "iTRAQ quantitative proteomics in analysis of vitreous humor from patients with retinal detachment". Paper presented in IV International Conference on Analytical Proteomics, 2015.
Journal article
  1. Santos, Fátima M.; Ciordia, Sergio; Mesquita, Joana; Cruz, Carla; Sousa, João Paulo Castro e; Passarinha, Luís A.; Tomaz, Cândida T.; Paradela, Alberto. "Proteomics profiling of vitreous humor reveals complement and coagulation components, adhesion factors, and neurodegeneration markers as discriminatory biomarkers of vitreoretinal eye diseases". Frontiers in Immunology 14 (2023): http://dx.doi.org/10.3389/fimmu.2023.1107295.
    Published • 10.3389/fimmu.2023.1107295
  2. dos Santos, Fátima Milhano; Ciordia, Sergio; Mesquita, Joana; de Sousa, João Paulo Castro; Paradela, Alberto; Tomaz, Cândida Teixeira; Passarinha, Luís António Paulino. "Vitreous humor proteome: unraveling the molecular mechanisms underlying proliferative and neovascular vitreoretinal diseases". Cellular and Molecular Life Sciences 80 1 (2022): http://dx.doi.org/10.1007/s00018-022-04670-y.
    Open access • Published • 10.1007/s00018-022-04670-y
  3. Khoramnia, Ramin; Peto, Tunde; Koch, Frank; Taylor, Simon R; Castro de Sousa, João Paulo; Hill, Lauren; Bailey, Clare; Chakravarthy, Usha. "Safety and effectiveness of the fluocinolone acetonide intravitreal implant (ILUVIEN): 3-year results from the European IRISS registry study". British Journal of Ophthalmology 107 10 (2022): 1502-1508. http://dx.doi.org/10.1136/bjo-2022-321415.
    10.1136/bjo-2022-321415
  4. Santos, Fátima Milhano; Mesquita, Joana; Castro-de-Sousa, João Paulo; Ciordia, Sergio; Paradela, Alberto; Tomaz, Cândida Teixeira. "Vitreous Humor Proteome: Targeting Oxidative Stress, Inflammation, and Neurodegeneration in Vitreoretinal Diseases". Antioxidants 11 3 (2022): 505. http://dx.doi.org/10.3390/antiox11030505.
    Open access • Published • 10.3390/antiox11030505
  5. Vaz-Pereira, S.; Castro-De-Sousa, J.P.; Martins, D.; Prates Canelas, J.; Reis, P.; Sampaio, A.; Urbano, H.; et al. "The Outcomes of Switching from Short- to Long-Term Intravitreal Corticosteroid Implant Therapy in Patients with Diabetic Macular Edema". Ophthalmic Research 63 2 (2020): 114-121. http://www.scopus.com/inward/record.url?eid=2-s2.0-85076299577&partnerID=MN8TOARS.
    10.1159/000503036
  6. Garweg, J.G.; Stefanickova, J.; Hoyng, C.; Schmelter, T.; Niesen, T.; Sowade, O.; Sivaprasad, S.; et al. "Vision-Related Quality of Life in Patients with Diabetic Macular Edema Treated with Intravitreal Aflibercept: The AQUA Study". Ophthalmology Retina 3 7 (2019): 567-575. http://www.scopus.com/inward/record.url?eid=2-s2.0-85070433860&partnerID=MN8TOARS.
    10.1016/j.oret.2019.03.012
  7. Santos, F.M.; Albuquerque, T.; Gaspar, L.M.; Dias, J.M.L.; Castro e Sousa, J.P.; Paradela, A.; Tomaz, C.T.; Passarinha, L.A.. "Refinement of two-dimensional electrophoresis for vitreous proteome profiling using an artificial neural network". Analytical and Bioanalytical Chemistry 411 20 (2019): 5115-5126. http://www.scopus.com/inward/record.url?eid=2-s2.0-85066633196&partnerID=MN8TOARS.
    10.1007/s00216-019-01887-y
  8. Chakravarthy, U.; Taylor, S.R.; Koch, F.H.J.; Castro De Sousa, J.P.; Bailey, C.. "Changes in intraocular pressure after intravitreal fluocinolone acetonide (ILUVIEN): Real-world experience in three European countries". British Journal of Ophthalmology 103 8 (2019): 1072-1077. http://www.scopus.com/inward/record.url?eid=2-s2.0-85053778659&partnerID=MN8TOARS.
    10.1136/bjophthalmol-2018-312284
  9. Joana Mesquita; João Paulo Castro-de-Sousa; Sara Vaz-Pereira; Arminda Neves; Luís A. Passarinha; Cândida T. Tomaz. "Evaluation of the growth factors VEGF-a and VEGF-B in the vitreous and serum of patients with macular and retinal vascular diseases". Growth Factors 36 1-2 (2018): 48-57. https://doi.org/10.1080/08977194.2018.1477140.
    10.1080/08977194.2018.1477140
  10. Campos, A.; Campos, E.J.; do Carmo, A.; Patrício, M.; Castro de Sousa, J.P.; Ambrósio, A.F.; Silva, R.. "Choroidal thickness changes stratified by outcome in real-world treatment of diabetic macular edema". Graefe's Archive for Clinical and Experimental Ophthalmology 256 10 (2018): 1857-1865. http://www.scopus.com/inward/record.url?eid=2-s2.0-85050556922&partnerID=MN8TOARS.
    10.1007/s00417-018-4072-z
  11. Sousa, João. "Vascular endothelial growth factors and placenta growth factor in retinal vasculopathies: Current research and future perspectives.". Cytokine & Growth Factor Reviews (2017): http://www.sciencedirect.com/science/article/pii/S1359610117301697?via%3Dihub.
    10.1016/j.cytogfr.2017.11.005
  12. Sousa, João. "Vegf-B Levels in the Vitreous of Diabetic and Non-Diabetic Patients with Ocular Diseases and Its Correlation with Structural Parameters". Medical Sciences (2017): http://www.mdpi.com/2076-3271/5/3/17.
    10.3390/medsci5030017
  13. Gaspar, L.M.; Santos, F.M.; Albuquerque, T.; Castro-de-Sousa, J.P.; Passarinha, L.A.; Tomaz, C.T.. "Proteome analysis of vitreous humor in retinal detachment using two different flow-charts for protein fractionation". Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 1061-1062 (2017): 334-341. http://www.scopus.com/inward/record.url?eid=2-s2.0-85026783759&partnerID=MN8TOARS.
    10.1016/j.jchromb.2017.07.049
  14. Neves, A.; Carvalheira, F.; Campos, J.; Alfaiate, P.; Campos, A.; Castro Sousa, J.P.. "Right Homonymous Hemianopia: A Clinical Case Report of Schizencephaly". Case Reports in Ophthalmology 7 1 (2016): 16-20. http://www.scopus.com/inward/record.url?eid=2-s2.0-84988683847&partnerID=MN8TOARS.
    10.1159/000443323
  15. Monteiro, J.P.; Santos, F.M.; Rocha, A.S.; Castro-de-Sousa, J.P.; Queiroz, J.A.; Passarinha, L.A.; Tomaz, C.T.. "Vitreous humor in the pathologic scope: Insights from proteomic approaches". Proteomics - Clinical Applications 9 1-2 (2015): 187-202. http://www.scopus.com/inward/record.url?eid=2-s2.0-84922796593&partnerID=MN8TOARS.
    10.1002/prca.201400133
  16. Campos, J.; Campos, A.; Mendes, S.; Neves, A.; Violante, L.; Castro Sousa, J.P.. "Premacular hemorrhage treatment with Nd:YAG laser: A clinical case,Tratamiento con láser Nd:YAG en una hemorragia premacular: Caso clínico". Archivos de la Sociedad Espanola de Oftalmologia 90 1 (2015): 44-46. http://www.scopus.com/inward/record.url?eid=2-s2.0-84923174380&partnerID=MN8TOARS.
    10.1016/j.oftal.2014.06.008
  17. Mendes, S.; Beselga, D.; Campos, S.; Neves, A.; Campos, J.; Carvalho, S.; Silva, E.; Castro Sousa, J.P.. "Possible rare congenital dysinnervation disorder: Congenital ptosis associated with adduction". Strabismus 23 1 (2015): 33-35. http://www.scopus.com/inward/record.url?eid=2-s2.0-84928007632&partnerID=MN8TOARS.
    10.3109/09273972.2014.999797
  18. Sousa, João. "A Rare Case of Retinal Artery Occlusion in the Context of Mediterranean Spotted Fever". Cases Reports in Ophthalmology (2014):
    10.1159/000358248
  19. Sousa, João. "Traumatic maculopathy 6 months after injury: a clinical case report.". Case reports in ophthalmology (2014):
    10.1159/000360692
  20. Rocha, A.S.; Santos, F.M.; Monteiro, J.P.; Castro-de-Sousa, J.P.; Queiroz, J.A.; Tomaz, C.T.; Passarinha, L.A.. "Trends in proteomic analysis of human vitreous humor samples". Electrophoresis 35 17 (2014): 2495-2508. http://www.scopus.com/inward/record.url?eid=2-s2.0-84906939868&partnerID=MN8TOARS.
    10.1002/elps.201400049
  21. Sousa, João. "Punctate Inner Choroidopathy: A Clinical Case Report". Case reports in Ophthalmology (2013): https://www.karger.com/Article/Abstract/355389#.
    10.1159/000355389
  22. Cunha-Vaz, J.; Castro Sousa, J.P.; Oliveiros, B.; Leite, E.; Lobo, C.; Castro Sousa, J.; Faria De Abreu, J.R.. "Progression of retinopathy and alteration of blood-retinal barrier in patients with type 2 diabetes. A seven year follow-up study". Investigative Ophthalmology and Visual Science 37 3 (1996): 264-268. http://www.scopus.com/inward/record.url?eid=2-s2.0-33750168701&partnerID=MN8TOARS.
    10.1007/s004170050075
  23. Cunha-Vaz, J.; Leite, E.; Castro Sousa, J.; Rui Faria de Abreu, J.. "Blood-retinal barrier permeability and its relation to progression of retinopathy in patients with type 2 diabetes. A four-year follow-up study". Graefe's Archive for Clinical and Experimental Ophthalmology 231 3 (1993): 141-145. http://www.scopus.com/inward/record.url?eid=2-s2.0-0027537399&partnerID=MN8TOARS.
    10.1007/BF00920936
  24. Sousa, P. Castro; Pinto, P.; Leite, E.; Cunha-Vaz, J. G.. "Digital subtraction macrodacryocystography by videotechnique". Documenta Ophthalmologica 80 4 (1992): 377-379. http://dx.doi.org/10.1007/bf00154387.
    10.1007/bf00154387
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2018/11 Development of an artificial neural network for vitreous protein profiling by bidimensional electrophoresis. 38th International Symposium on the Purification of Proteins, Peptides and Polynucleotides
Austrian Centre of Industrial Biotechnology (Berlim, Germany)
2018/07 Quantitative analysis of vitreous from patients with retinal detachment using iTRAQ-based proteomics, XIII Annual CICS Symposium
Universidade da Beira Interior (Covilhã, Portugal)
2018/07 Evaluation of vitreous and serum VEGF-A, VEGF-B and PIGF in angiogenic ocular disorders, XIII Annual CICS Symposium
Universidade da Beira Interior (Covilhã, Portugal)
2017/07 Enhancement of bidimensional electrophoretic operation conditions by an artificial neural network for vitreous humor protein analysis. V International Conference onAnalytical Proteomics
UCIBIO-REQUIMTE (Costa da Caparica, Portugal)
2017/07 Quantification and comparison of VEGF-A, VEGF-B and Placental Growth Factor (PlGF) in the Vitreous Humor and Plasma of patients with ocular pathology: Correlation between cytokines and clinical parameters XII CICS Symposium
Universidade da Beira Interior (Covilhã, Portugal)
2016/06 iTRAQ quantitative proteomics in analysis of vitreous humor from patients with Retinal Detachment. XI ANNUAL CICS-UBI SYMPOSIUM
Universidade da Beira Interior (Covilhã, Portugal)
2014/11 Preliminary results on quantitative proteome analysis of vitreous humor samples using iTRAQ. II jornadas de Toxicologia
Universidade da Beira Interior (Covilhã, Portugal)
2013/06 Strategies to Optimize the Sample Preparation for Proteomic Analysis of Vitreous Humor in Retinal Pathologies. SIRCOVA Intenational Meeting 2013
Sociedad de Investigación de retina y Ciencias de la visión (Valencia, Spain)
Distinctions

Other distinction

1990 Prémio de Cirurgia Implanto-Refractiva
Sociedade Portuguesa de Oftalmologia, Portugal