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I consider myself a hardworking person, whose main areas of interest are Microbiology, Molecular Biology, Immunology and Antibiotic Resistance, with a view to applied drug development and Public Health. I obtained a bachelor's degree in Cellular and Molecular Biology by FCT-UNL in 2018, with a final classification of 16/20, after I successfully presented my bachelor project "Construção de um mutante knockout do namH em Mycobacterium smegmatis e construção de um plasmídeo para expressar a proteína NamH". After completing my bachelor internship at the Mycobacterial Pathogenesis lab (Prof. Maria João Catalão, Faculty of Pharmacy), my interest in mycobacteria as unique and extremely complex living beings led me to pursue my master studies in this area. Tuberculosis, the deadliest infectious disease in the world, which is estimated to kill 1.5 million people annually, is a global health emergency due to the emergence of multidrug, extensively-drug and totally-drug resistant strains of Mycobacterium tuberculosis. The peptidoglycan (PG) layer of the mycobacteria is specifically relevant since it features unique modifications, of which the N-glycolylation of PG muramic acid, catalyzed by the activity of the N-acetyl muramic acid hydroxylase (NamH), is of special interest. Therefore, my master thesis was focused on studying the role of the N-glycolylation of PG in host immune recognition and in antibiotic resistance. To do so, I have constructed several namH knockdown mutants in both M. smegmatis and Mycobacterium tuberculosis H37Ra using CRISPR interference. Through hard-work and perseverance, I have optimized various experimental procedures and obtained some interesting results in the scope of antibiotic resistance and host-pathogen interactions during my master thesis. I presented and discussed my dissertation on the 4th of January of 2021, having obtained the final classification of 19/20. As a result, I have completed the master course of Biopharmaceutical Sciences by the Faculty of Pharmacy of the University of Lisbon in January 2021, with a final classification of 19/20 and one Merit Award. In the meantime, I have remained at the same laboratory as a Research Collaborator and started to investigate the potential activity of NamH as a Metallo-ß-lactamase, as hypothesized in my master's dissertation. Currently, I am pursuing a Ph.D. in Pharmacy, with a specialization in Microbiology. During the Ph.D. program, I plan to continue my previous lines of research. I intend to further the study of the relevance of the unique modifications of mycobacterial peptidoglycan (N-glycolylation of muramic acids, amidation of D-isoglutamate and amidation of m-DAP) in the antibiotic resistance and in the host-pathogen interactions of Mycobacterium tuberculosis. In the future, I will work to achieve my goals, which include having professional experience abroad and teaching.
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Identification

Personal identification

Full name
Cátia Raquel Lopes Silveiro
Date of birth
1997/07/27
Gender
Female

Citation names

  • Silveiro, Cátia

Author identifiers

Ciência ID
DB18-1CAD-A7C6
ORCID iD
0000-0003-3052-6372

Addresses

  • Faculdade de Farmácia da Universidade de Lisboa. Avenida Professor Gama Pinto, 1649-003, Lisboa, Lisboa, Portugal (Professional)

Websites

Knowledge fields

  • Natural sciences - Biological Sciences - Microbiology
  • Medical and Health Sciences - Health Sciences - Infectious Diseases
  • Natural sciences - Biological Sciences - Molecular Biology

Languages

Language Speaking Reading Writing Listening Peer-review
English Advanced (C1) Proficiency (C2) Advanced (C1) Proficiency (C2) Advanced (C1)
Portuguese (Mother tongue)
Education
Degree Classification
2021/10/01 - 2025/10/01
Ongoing
 Farmácia (Doutoramento)
Major in Microbiologia
Universidade de Lisboa Faculdade de Farmácia, Portugal
"Deciphering the role of mycobacterial peptidoglycan modifications in host immune recognition and antibiotic resistance of Mycobacterium tuberculosis " (THESIS/DISSERTATION)
2018/09 - 2021/01/04
Concluded
 Ciências Biofarmacêuticas (Mestrado)
Major in Biologia Celular e Molecular; Microbiologia; Interações patogénio-hospedeiro
Universidade de Lisboa Faculdade de Farmácia, Portugal
"Role of the N-glycolylation of the mycobacterial peptidoglycan in host immune recognition and antibiotic resistance" (THESIS/DISSERTATION)
 19
2015/09 - 2018/06
Concluded
 Biologia Celular e Molecular (Licenciatura)
Major in Biologia Molecular
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
"Construção de um mutante de deleção do gene namH em Mycobacterium smegmatis e construção de um plasmídeo para sobreexpressar a proteína NamH" (THESIS/DISSERTATION)
 16
2012/09 - 2015/06
Concluded
 Ciências e Tecnologias (Ensino secundário)
Escola Secundária de São João da Talha, Portugal
 16,2
Affiliation

Science

Category
Host institution 		Employer
2021/02/01 - 2021/09/30 Research Trainee (Research) Universidade de Lisboa Faculdade de Farmácia, Portugal
Universidade de Lisboa Instituto de Investigação do Medicamento, Portugal
Projects

Contract

Designation Funders
2021/02 - 2021/09 Dissecting Mycobacterium tuberculosis complex cell wall structure and modifications: contribution to host immune recognition and drug resistance
IF/00414/2015
Researcher
Universidade de Lisboa Instituto de Investigação do Medicamento, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2019/09/10 - 2021/01/04 Clinical Significance of Mycobacterium tuberculosis cell wall structural diversity: contribution to novel mechanisms of antibiotic resistance and relevance towards tuberculosis progression and treatment
PTDC/BIA-MIC/31233/2017
Tese de Mestrado
Universidade de Lisboa Instituto de Investigação do Medicamento, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2019/09/10 - 2021/01/04 Dissecting Mycobacterium tuberculosis complex cell wall structure and modifications: contribution to host immune recognition and drug resistance
IF/00414/2015
Tese de Mestrado
Universidade de Lisboa Instituto de Investigação do Medicamento, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2018/01/20 - 2018/04/28 Decoding the peptidoglycan patterns associated with beta-lactams hypersusceptibility in drug-resistant Mycobacterium tuberculosis
ESCMID 2018
Estagiária
Universidade de Lisboa Instituto de Investigação do Medicamento, Portugal
 European Society of Clinical Microbiology and Infectious Diseases
Concluded
Outputs

Publications

Conference poster
  1. Silveiro, C.; Olivença, Francisco; Nunes, Alexandra; ANES, ELSA; Catalão, Maria João. "CRISPRi-mediated depletion of distinctive PG modifications promotes beta-lactam hypersusceptibility phenotypes in M. tuberculosis". 2023.
  2. Olivença, Francisco; Alexandra Nunes; Rita Macedo; David Pires; Silveiro, Cátia; Cláudia Ferreira; Maria Miragaia; et al. "WGS-based Dual Strategy Identifies Lipoproteins with PBP Activity as Potential Targets to Enhance Beta-lactam Activity in Mycobacterium tuberculosis". Paper presented in Young Researcher Day INSA 2022, 2022.
  3. Olivença, Francisco; Alexandra Nunes; Rita Macedo; David Pires; Silveiro, Cátia; Cláudia Ferreira; Maria Miragaia; et al. "WGS-based Dual Strategy for the Identification of Key Targets to Enhance Beta-lactam Activity in Mycobacterium tuberculosis". Paper presented in Conference on Drug Development to Meet the Challenge of AMR 2022, 2022.
  4. Silveiro, C.; Marques, Mariana; Olivença, Francisco; Pires, David; ANES, ELSA. "The quest for novel targets in TB-therapy: CRISPRi-mediated repression of PG modifications in Mycobacterium smegmatis promotes beta-lactam susceptibility and intracellular killing". Paper presented in EMBO Workshop on Tuberculosis 2022, 2022.
  5. Silveiro, C.; Marques, Mariana; Olivença, Francisco; Catalão, Maria João. "CRISPRi-mediated repression of peptidoglycan modifications in Mycobacterium smegmatis leads to ß-lactam hypersusceptibility phenotypes". Paper presented in iMed.ULisboa Meeting 2022, 2022.
  6. Silveiro, C.; Marques, Mariana; Olivença, Francisco; Catalão, Maria João. "Depletion of peptidoglycan modifications sensitizes Mycobacterium smegmatis to Beta-lactam antibiotics". Paper presented in ECCMID 2022, 2022.
  7. Olivença, Francisco; Ferreira, Cláudia; Silveiro, Cátia; Nunes, Alexandra; Gomes, João Paulo; Catalão, Maria João. "Anticipating Mycobacterial Beta-lactam Resistance: Do we know the whole story?". Paper presented in 32nd European Congress of Clinical Microbiology and Infectious Diseases, 2022.
  8. Olivença, Francisco; Nunes, Alexandra; Macedo, Rita; Pires, David; Silveiro, Cátia; ANES, ELSA; João Paulo Gomes; Catalão, Maria João. "Decoding Beta-lactam Susceptibility Patterns in Mycobacterium tuberculosis Clinical Strains". Paper presented in 32nd European Congress of Clinical Microbiology and Infectious Diseases, 2022.
  9. Silveiro, Cátia; Marques, Mariana; Olivença, Francisco; Catalão, Maria João. "Depletion of peptidoglycan modifications sensitizes Mycobacterium smegmatis to ß-lactam antibiotics". Paper presented in 32nd European Congress of Clinical Microbiology and Infectious Diseases, 2022.
  10. Olivença, Francisco; Alexandra Nunes; Macedo, Rita; David Pires; Silveiro, Cátia; Anes, Elsa; João Paulo Gomes; Maria João Catalão. "Repurposing Beta-lactams in Tuberculosis: a Valuable Therapeutic Alternative Against Drug-Resistant Clinical Isolates of Mycobacterium tuberculosis". Paper presented in NOVOS DESAFIOS EM SÁUDE NA ULISBOA, 2021.
  11. Silveiro, Cátia; Marques, Mariana; Olivença, Francisco; Pires, David; ANES, ELSA; Catalão, Maria João (5114-A729-B411). Corresponding author: Catalão, Maria João (5114-A729-B411). "A novel target in TB-therapy: the N-glycolylation of mycobacterial peptidoglycan contributes to antibiotic resistance and intracellular survival". Paper presented in Microbiotec, 2021.
  12. Olivença, Francisco; Alexandra Nunes; David Pires; Silveiro, Cátia; Ferreira, Cláudia; Rita Macedo; Elsa Anes; João Paulo Gomes; Catalão, Maria João. "The Combination of Carbapenems and Clavulanic Acid Efficiently Inhibits Mycobacterium tuberculosis Clinical Isolates". Paper presented in iMed.ULisboa Meeting 2021, 2021.
  13. Silveiro, Cátia; Marques, Mariana; Carmo, Nuno; Olivença, Francisco; Pires, David; Ferreira, Claúdia; ANES, ELSA; Catalão, Maria João. Corresponding author: Catalão, Maria João. "Relevance of the N-glycolylation of mycobacterial peptidoglycan to antibiotic resistance and to host-pathogen interactions". Paper presented in iMed.ULisboa Meeting 2021, 2021.
  14. Silveiro, Cátia; Marques, Mariana; Carmo, Nuno; Olivença, Francisco; Catalão, Maria João. "Significance of the N-glycolylation of mycobacterial peptidoglycan in antibiotic resistance". Paper presented in II Simpósio em Tuberculose e Micobactérias Não Tuberculosas em Portugal, 2020.
  15. Marques, Mariana; Silveiro, Cátia; Carmo, Nuno; Olivença, Francisco; Catalão, Maria João. "Significance of the Amidation of Mycobacterial Peptidoglycan in Antibiotic Resistance". Paper presented in II Simpósio em Tuberculose e Micobactérias Não Tuberculosas em Portugal, 2020.
Journal article
  1. Silveiro, Cátia; Marques, Mariana; Olivença, Francisco; Pires, David; Mortinho, Diana; Nunes, Alexandra; Pimentel, Madalena; Anes, Elsa; Catalão, Maria João. "CRISPRi-mediated characterization of novel anti-tuberculosis targets: Mycobacterial peptidoglycan modifications promote beta-lactam resistance and intracellular survival". Frontiers in Cellular and Infection Microbiology 13 (2023): 1-20. https://www.frontiersin.org/articles/10.3389/fcimb.2023.1089911/full.
    Open access • Published • 10.3389/fcimb.2023.1089911
  2. Olivença, Francisco; Ferreira, Cláudia; Nunes, Alexandra; Silveiro, Cátia; Pimentel, Madalena; Gomes, João Paulo; Catalão, Maria João. "Identification of drivers of mycobacterial resistance to peptidoglycan synthesis inhibitors". Frontiers in Microbiology 13 (2022): http://dx.doi.org/10.3389/fmicb.2022.985871.
    Published • 10.3389/fmicb.2022.985871
  3. Olivença, Francisco; Nunes, Alexandra; Macedo, Rita; Pires, David; Silveiro, Cátia; ANES, ELSA; Miragaia, Maria ; Gomes, João Paulo; Catalão, Maria João. Corresponding author: Catalão, Maria João. "Uncovering Beta-Lactam Susceptibility Patterns in Clinical Isolates of Mycobacterium tuberculosis through Whole-Genome Sequencing". Microbiology Spectrum (2022): http://dx.doi.org/10.1128/spectrum.00674-22.
    Open access • Published • 10.1128/spectrum.00674-22
Preprint
  1. Francisco Olivença; David Pires; Cátia Silveiro; Bianca Gama; Frederico Holtreman; Elsa Anes; Maria João Catalão. "Ethambutol and Meropenem/Clavulanate Synergy Promotes Enhanced Extracellular and Intracellular Killing ofMycobacterium tuberculosis". 2023. https://doi.org/10.1101/2023.10.24.563807.
    10.1101/2023.10.24.563807
Report
  1. Silveiro, Cátia; Catalão, Maria João. 2019. Role of the N-glycolylation of the mycobacterial peptidoglycan in host immune recognition and antibiotic resistance. https://drive.google.com/file/d/1lPKyLoG5LHqBM0yBUtvzQcfZSws5MQl5/view?usp=sharing.
  2. Silveiro, Cátia; Catalão, Maria João. 2019. CRISPRi, a tool to investigate bacterial virulence and to unravel novel drug targets. https://drive.google.com/file/d/1Q277hvtM8fxgnoePT6cOlWf-K_e5CJai/view?usp=sharing.
  3. Silveiro, Cátia; Olivença, Francisco; Catalão, Maria João. 2018. Construction of a deletion mutant of the namH gene in Mycobacterium smegmatis and construction of a plasmid to express the protein NamH. https://drive.google.com/file/d/190LV3TXa2a_Oub8wFuRsMOwCa9oU5Sws/view?usp=sharing.
Thesis / Dissertation
  1. Silveiro, Cátia. "Role of the N-glycolylation of mycobacterial peptidoglycan in host immune recognition and antibiotic resistance". Master, Universidade de Lisboa Faculdade de Farmácia, 2021. https://drive.google.com/file/d/1-6VjfeNARaL24fVTX7r53q0YeB6Ci5se/view?usp=sharing.
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2023/06/26 CRISPRi-mediated silencing of characteristic peptidoglycan modifications in M. smegmatis promotes beta-lactam susceptibility and intracellular killing
ipSC (Lisboa, Portugal)
2021/06/16 Relevance of the N-glycolylation of mycobacterial peptidoglycan to antibiotic resistance and to host-pathogen interactions iMed.ULisboa Meeting 2021
ipSC (Lisboa, Portugal)

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2022/10 - 2023/09 Studies on mycobaterial glycosyltransferases, promising targets to develop antituberculosis drugs
Co-supervisor of Bárbara Rodrigues
 Biotecnologia (Master)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal

Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Universidade de Lisboa Faculdade de Farmácia, Portugal
2021/10/01 - 2023/01 Reappraising the use of beta-lactam antibiotics against tuberculosis: study of genes associated with beta-lactam susceptibility in mycobacteria
Co-supervisor of Diana Mortinho
 Microbiologia Aplicada (Master)
Universidade de Lisboa Faculdade de Ciências, Portugal

Universidade de Lisboa Faculdade de Farmácia, Portugal
2022/03/01 - 2022/07/27 Construction and characterization of a CRISPRi-mediated knockdown mutant library of genes previously identified as relevant for differential beta-lactam susceptibility phenotypes in Mycobacterium
Co-supervisor of Mariana Pinho
 Ciências Farmacêuticas (Master)
Universidade de Lisboa Faculdade de Farmácia, Portugal
2022/03/01 - 2022/06 Construction and characterization of a CRISPRi-mediated knockdown mutant library of genes previously identified as relevant for differential beta-lactam susceptibility phenotypes in Mycobacterium
Co-supervisor of Beatriz Paquito
 Ciências Farmacêuticas (Master)
Universidade de Lisboa Faculdade de Farmácia, Portugal
2021/10/01 - 2021/12/16 Uncovering the role of papA1 in the antibiotic susceptibility of Mycobacterium smegmatis
Co-supervisor of Diogo António da Costa Fevereiro
 Ciências Farmacêuticas (Master)
Universidade de Lisboa Faculdade de Farmácia, Portugal
2021/10/01 - 2021/12/16 Uncovering the role of cut3 in the antibiotic susceptibility of Mycobacterium smegmatis
Co-supervisor of Carolina Sofia de Sousa Pinheiro dos Santos Martins
 Ciências Farmacêuticas (Master)
Universidade de Lisboa Faculdade de Farmácia, Portugal
2021/10/01 - 2021/12/16 Purification of a mCherry Fluorescent Fusion of the D29 Mycobacteriophage Endolysin Cell Wall Binding Domain for Peptidoglycan Binding Studies
Co-supervisor of Mariana Ruiz Estrela
 Ciências Farmacêuticas (Master)
Universidade de Lisboa Faculdade de Farmácia, Portugal
2021/02/01 - 2021/05 Characterization of NamH beta-lactamase activity in Mycobacterium smegmatis
Co-supervisor of Inês Soares
 Biologia Celular e Molecular (Degree)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal

Event participation

Activity description
Type of event 		Event name
Institution / Organization
2023/06/26 - Current 14th iMed.Ulisboa Postgraduate Students Meeting, 2023
Conference
Universidade de Lisboa Faculdade de Farmácia, Portugal
2022/09/12 - 2022/09/16 EMBO Workshop on Tuberculosis 2022
Workshop
 EMBO Workshop on Tuberculosis 2022
Gesellschaft zur Förderung der Lebenswissenschaften Heidelberg GmbH, Germany

Institut Pasteur Centre Médical, France
2022/07/04 - 2022/07/05 iMed.ULisboa Meeting 2022
Conference
 iMed.ULisboa Meeting 2022
Universidade de Lisboa Faculdade de Farmácia, Portugal
2022/05/16 - 2022/05/18 Encontro Ciência 2022
Conference
Fundação para a Ciência e a Tecnologia, Portugal
2022/04/23 - 2022/04/26 ECCMID 2022
Conference
 ECCMID 2022
European Society of Clinical Microbiology and Infectious Diseases, Switzerland
2021/11/23 - 2021/11/26 Microbiotec 2021
Conference
 Microbiotec 2021
2021/06/16 - 2021/07/09 iMed.ULisboa Meeting 2021
Conference
 iMed.ULisboa Meeting 2021
ipSC, Portugal
2020/01/31 - 2020/01/31 2º Simpósio de Investigação em Tuberculose e Micobactérias Não Tuberculosas em Portugal
Symposium
 2º Simpósio de Investigação em Tuberculose e Micobactérias Não Tuberculosas em Portugal
Universidade de Lisboa Faculdade de Farmácia, Portugal
2018/12/13 - 2018/12/14 Innovative approaches in targeted therapies: Brain, Infections and Cancer
Workshop
 Innovative approaches in targeted therapies: Brain, Infections and Cancer
Universidade de Lisboa Faculdade de Medicina, Portugal
2018/07/24 - 2018/07/25 10th iMed.ULisboa and 3rd i3du Postgraduate Students Meeting
Conference
 10th iMed.ULisboa and 3rd i3du Postgraduate Students Meeting
Universidade de Lisboa Faculdade de Farmácia, Portugal
2017/11/29 - 2017/11/29 Conversas: Neurobiologia
Seminar
 Conversas: Neurobiologia
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2017/02/21 - 2017/02/23 5ª edição das Jornadas de Investigação em Biologia
Seminar
 5ª edição das Jornadas de Investigação em Biologia
Fundação da Faculdade de Ciências da Universidade de Lisboa, Portugal
2015/11/04 - 2015/11/04 Limites da Ciência
Seminar
 Limites da Ciência
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal

Mentoring / Tutoring

Topic Student name
2022/10 - 2023/09 Studies on mycobacterial glycosyltransferases, promising targets to develop antituberculosis drugs Bárbara Rodrigues
2021/10/01 - 2023/01 Reappraising the use of beta-lactam antibiotics against tuberculosis: study of genes associated with beta-lactam susceptibility in mycobacteria Diana Mortinho
2022/03/01 - 2022/07/27 Construction and characterization of a CRISPRi-mediated knockdown mutant library of genes previously identified as relevant for differential beta-lactam susceptibility phenotypes in Mycobacterium Mariana Pinho
2022/03/01 - 2022/07/27 Construction and characterization of a CRISPRi-mediated knockdown mutant library of genes previously identified as relevant for differential beta-lactam susceptibility phenotypes in Mycobacterium Beatriz Paquito
2021/10/01 - 2021/12/16 Uncovering the role of papA1 in the antibiotic susceptibility of Mycobacterium smegmatis Diogo António da Costa Fevereiro
2021/10/01 - 2021/12/16 Uncovering the role of cut3 in the antibiotic susceptibility of Mycobacterium smegmatis Carolina Sofia de Sousa Pinheiro dos Santos Martins
2021/10/01 - 2021/12/16 Purification of a mCherry Fluorescent Fusion of the D29 Mycobacteriophage Endolysin Cell Wall Binding Domain for Peptidoglycan Binding Studies Mariana Ruiz Estrela
2021/02/01 - 2021/06/05 Characterization of NamH beta-lactamase activity in Mycobacterium smegmatis Inês Soares
Distinctions

Award

2020 Bolsa de Mérito
Universidade de Lisboa Faculdade de Farmácia, Portugal

Republica Portuguesa Ministério da Educação, Portugal

Other distinction

2021 C2 Proficiency
Cambridge English Language Assessment Spain and Portugal, Spain