Personal identification

Full name

Mariana Oliveira Nunes

Date of birth

1992/01/04

Gender

Female

Citation names

• Nunes, Mariana
• Nunes, M

Author identifiers

Ciência ID

DC17-6C7C-5869

ORCID iD

0000-0002-4733-5778

Researcher Id

rid90383

Scopus Author Id

57217494194

Email addresses

• mnunes@i3s.up.pt (Professional)
• marianaoliveiranunes1992@gmail.com (Personal)

Websites

• linkedin.com/in/mariana-nunes-139a61188 (Social media)

Knowledge fields

• Medical and Health Sciences - Clinical Medicine - Oncology
• Natural sciences - Biological Sciences - Biology

Languages

Language	Speaking	Reading	Writing	Listening	Peer-review
Portuguese (Mother tongue)
English	Intermediate (B1)	Advanced (C1)	Advanced (C1)	Advanced (C1)

	Degree	Classification
2020/11/01 - 2024/11 Ongoing	Patologia e Genética Molecular (Doutoramento) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2017/09 - 2019/09 Concluded	Mestrado em Oncologia (Mestrado) Major in Oncologia Laboratorial Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal "First line drug testing in ovarian cancer cell lines manipulated for Mesothelin expression" (THESIS/DISSERTATION)	18
2011/09 - 2016/06 Concluded	Biologia (Licenciatura) Universidade de Aveiro, Portugal "Biochemical characterization of the interaction phytoprotectors/phytopathogens in grapevine" (THESIS/DISSERTATION)	15

Science

	Category Host institution	Employer
2019/11/01 - 2020/10/31	Researcher (Research)	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal

Contract

	Designation	Funders
2024/04/22 - 2025/04/21	Exploring the effectiveness of pitavastatin to potentiate immunotherapy using ovarian cancer ascites immune-organoid cultures ImmunoT4OC_GI2- CESPU-2023 Researcher Cooperativa de Ensino Superior Politécnico e Universitário, Portugal Instituto Universitário de Ciências da Saúde Unidade de Investigação em Toxicologia, Portugal	Ongoing
2020/11/01 - 2024/10/31	Drug Repurposing as a Strategy to Overcome Ovarian Cancer Chemoresistance 2020.04720.BD PhD Student Fellow Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2022/09/01 - 2023/08/31	Ovarian Cancer Ascites-Derived Organoids as a Preclinical Platform to Predict Therapeutical Responses OVCARTEST_GI2-CESPU_2022 Researcher Cooperativa de Ensino Superior Politécnico e Universitário, Portugal Instituto Universitário de Ciências da Saúde Unidade de Investigação em Toxicologia, Portugal	Cooperativa de Ensino Superior Politécnico e Universitário Concluded
2022/09/01 - 2023/08/31	Overcoming Paclitaxel Resistance in Ovarian Cancer upPTXovcar-GI2-CESPU-2022 Researcher Cooperativa de Ensino Superior Politécnico e Universitário, Portugal Instituto Universitário de Ciências da Saúde Unidade de Investigação em Toxicologia, Portugal	Cooperativa de Ensino Superior Politécnico e Universitário Concluded
2019/11/01 - 2020/10/31	Bloqueio da interacção MUC16-mesotelina para inibir a metastização peritoneal do carcinoma do ovário PTDC/MEC-ONC/29503/2017 Master Student Fellow Universidade do Porto Instituto de Patologia e Imunologia Molecular, Portugal	Fundação para a Ciência e a Tecnologia Concluded

Publications

Book chapter	Nunes, Mariana; Almeida-Nunes, Diana Luísa; Ricardo, Sara. "Cell Microarray: an approach to evaluate drug-induced alterations in protein expression". In Advancements in Cancer Research, 133-144. Exon Publications, 2023. 10.36255/cell-microarray Nunes, Mariana; Ricardo, Sara. "Chemoresistance in ovarian cancer: the role of malignant ascites". In Ovarian Cancer, 27-42. Exon Publications, 2022. Published • 10.36255/exon-publications-ovarian-cancer-malignant-ascites
Conference poster	Nunes, Mariana; Ricardo, Sara. "Repurposing Ivermectin to Enhance Chemotherapy’s Effectiveness in High-Grade Serous Carcinoma". Paper presented in EACR 2024 Congress: Innovative Cancer Science, 2024. Almeida-Nunes, Diana Luísa; Mendes-Frias, Ana; Nunes, Mariana; Ferreira, Verónica; Lobo, Cláudia; Monteiro, Paula; Abreu, Miguel Henriques; et al. "Presence of exhausted lymphocytes in the ascitic microenvironment of ovarian cancer patients: implications for immune dysfunction". Paper presented in VII iBiMED Symposium: Sharing Science, Inspiring Progress, 2024. Nunes, Mariana; Costa, João; Bartosch, Carla; Ricardo, Sara. "The Expression of mRNA LGR6 is Increased in Low-Grade Serous Carcinoma". Paper presented in XXX Porto Cancer Meeting: RNA Biology in Cancer, 2024. Almeida-Nunes, Diana Luísa; Nunes, Mariana; Osorio, Hugo; Ferreira, Verónica; Lobo, Cláudia; Monteiro, Paula; Abreu, Miguel Henriques; et al. "Análise proteómica de ascites de pacientes com cancro do ovário reflete a modificação metabólica no microambiente tumoral durante a quimioterapia". Paper presented in 8º Curso Avançado de Ginecologia Oncológica, 2023. Nunes, Mariana; Almeida-Nunes, Diana Luísa; Costa, Anabela; Ricardo, Sara. "Cell microarray as a powerful tool to accelerate cancer research: focus on drug screening". Paper presented in II TOXRUN INTERNATIONAL CONGRESS 2023, 2023. Nunes, Mariana; Duarte, Diana; Vale, Nuno; Ricardo, Sara. "Synergism between carboplatin and repurposed drugs in chemoresistant high-grade serous carcinoma". Paper presented in EACR: The invisible phase of metastasis: Impact of dormancy, cancer cell plasticity and evolution of the niche during metastatic progression - Virtual Event, 2022. Nunes, Mariana; Almeida-Nunes, Diana Luísa; Abreu, Miguel Henriques; Bartosch, Carla; Ricardo, Sara. "Recycling the discarded ovarian cancer ascites to monitor therapy resistance". Paper presented in 23rd European Congress on Gynaecological Oncology, 2022. Nunes, Mariana; Duarte, Diana; Vale, Nuno; Ricardo, Sara. "Combining repurposing drugs with paclitaxel has a synergic effect in chemoresistant high-grade serous carcinoma". Paper presented in 5th ASPIC International Congress, 2022. Brandão, Catarina; Almeida-Nunes, Diana Luísa; Nunes, Mariana; Osório, Hugo; Ricardo, Sara. "Development of an in silico approach to identify key proteins involved in the peritoneal dissemination of ovarian cancer". Paper presented in VII Jornadas da Bioquímica, 2022. Nunes, Mariana; Brandão, Catarina; Ricardo, Sara. "Combining paclitaxel with repurposing drugs improve ovarian cancer management". Paper presented in I Congresso Internacional da TOXRUN/ V Congresso da Associação Portuguesa de Ciências Forenses/XIV Jornadas Científicas de Ciências do Instituto Universitário de Ciências da Saúde, 2022. Ferreira, Sílvia; Nunes, Mariana; Almeida-Nunes, Diana Luísa; Ricardo, Sara. "Phenotypic Evaluation of the Ovarian Tumor Cells Presents in Malignant Ascites". Paper presented in I Congresso Internacional da TOXRUN/ V Congresso da Associação Portuguesa de Ciências Forenses/XIV Jornadas Científicas de Ciências do Instituto Universitário de Ciências da Saúde, 2022. Nunes, Mariana; Ricardo, Sara. "Pitavastatin partially restore paclitaxel sensitivity in high-grade serous carcinoma cell lines". Paper presented in EACR Conference 2021: Goodbye Flat Biology: Next Generation Cancer Models, virtual event, worldwide., 2021. Coelho, Ricardo; Ricardo, Sara; Amaral, Ana Luísa; Huang, Yen-Lin; Nunes, Mariana; Jacob, Francis; David, Leonor. "Mesothelin-expressing ovarian cancer cells exhibit enhanced invasion and peritoneal dissemination". Paper presented in EACR-AACR-ASPIC Conference 2020: Tumor Microenvironment, 2020. Nunes, Mariana; Coelho, Ricardo; David, Leonor; Ricardo, Sara. "A mesotelina modula a quimioresistência à carboplatina em linhas celulares do cancro do ovário". Paper presented in 1º Encontro Nacional de Jovens Investigadores em Oncologia, 2019. Nunes, Mariana; Coelho, Ricardo; Portugal, Raquel; Costa, Antonia; Lopes, José Manuel; David, Leonor; Ricardo, Sara. "Reduced expression of MUC16 and MSLN in cells from ascitic fluid and ovarian cancer cell lines". Paper presented in 7th i3S Annual Meeting, 2018. Félix, Carina; Nunes, Mariana; Andolfi, Anna; DellaGreca, Marina; Basso, S; Salvatore, Maria M; Correia, António; Alves, Artur; Esteves, Ana Cristina. "The effect of temperature on the expression of extracellular enzymes of Lasiodiplodia theobromae". Paper presented in IV Encontro Nacional de Pós-Graduação em Ciências Biológicas, 2015. Nunes, Mariana; Félix, Carina; Meneses, Rodrigo; Correia, António; Alves, Artur; Esteves, Ana Cristina. "Effect of temperature on the plant pathogen Lasiodiplodia theobromae: enzyme and secondary metabolites production". Paper presented in XVII Congress of European Mycologists, 2015. Félix, Carina; Nunes, Mariana; Correia, António; Alves, Artur; Esteves, Ana Cristina. "How do phytopathogenic fungi behave under a climate change scenario?". Paper presented in RS. Research day, 2015.
Journal article	Almeida-Nunes, Diana Luisa; Silva, João Pedro Nunes; Nunes, Mariana; Silva, Patrícia M. A.; Silvestre, Ricardo; Dinis-Oliveira, Ricardo Jorge; Bousbaa, Hassan; Ricardo, Sara. "Metformin Impairs Linsitinib Anti-Tumor Effect on Ovarian Cancer Cell Lines". International Journal of Molecular Sciences (2024): https://doi.org/10.3390/ijms252211935. 10.3390/ijms252211935 Nunes, Mariana; Almeida-Nunes, Diana Luísa; Ferreira, Verónica; Lobo, Cláudia; Monteiro, Paula; Abreu, Miguel Henriques; Bartosch, Carla; Ricardo, Sara. "Que outra utilidade clínica poderão ter as ascites no carcinoma do ovário?". Revista Portuguesa de Oncologia 7 1-2 (2024): Nunes, Mariana; Bartosch, Carla; Abreu, Miguel Henriques; Richardson, Alan; Almeida, Raquel; Ricardo, Sara. "Deciphering the Molecular Mechanisms behind Drug Resistance in Ovarian Cancer to Unlock Efficient Treatment Options". Cells (2024): https://doi.org/10.3390/cells13090786. 10.3390/cells13090786 Nunes, Mariana; Duarte, Diana; Vale, Nuno; Ricardo, Sara. "The antineoplastic effect of carboplatin is potentiated by combination with pitavastatin or metformin in a chemoresistant high-grade serous carcinoma cell line". International Journal of Molecular Sciences (2022): https://doi.org/10.3390/ijms24010097. 10.3390/ijms24010097 Duarte, Diana; Nunes, Mariana; Ricardo, Sara; Vale, Nuno. "Combination of antimalarial and CNS drugs with antineoplastic agents in MCF-7 breast and HT-29 colon cancer cells: biosafety evaluation and mechanism of action". Biomolecules 12 10 (2022): 1490-1490. http://dx.doi.org/10.3390/biom12101490. 10.3390/biom12101490 Nunes, Mariana; Duarte, Diana; Vale, Nuno; Ricardo, Sara. "Pitavastatin and ivermectin enhance the efficacy of paclitaxel in chemoresistant high-grade serous carcinoma". Cancers (2022): https://doi.org/10.3390/cancers14184357. 10.3390/cancers14184357 Magalhães, Ana C.; Ricardo, Sara; Moreira, Ana C. ; Nunes, Mariana; Tavares, Margarida ; Pinto, Ricardo J.; Gomes, Maria Salomé; Pereira, Luisa. "InfectionCMA: a cell microArray approach for efficient biomarker screening in in vitro infection assays". Pathogens (2022): https://doi.org/10.3390/pathogens11030313. 10.3390/pathogens11030313 Nunes, Mariana; Pacheco, Francisca; Coelho, Ricardo; Leitão, Dina; Ricardo, Sara; David, Leonor. "Mesothelin expression is not associated with the presence of cancer stem cell markers SOX2 and ALDH1 in ovarian cancer". International Journal of Molecular Sciences 23 3 (2022): 1016-1016. http://dx.doi.org/10.3390/ijms23031016. 10.3390/ijms23031016 Nunes, Mariana; Silva, Patrícia M. A.; Coelho, Ricardo; Pinto, Carla; Resende, Albina; Bousbaa, Hassan; Almeida, Gabriela M.; Ricardo, Sara. "Generation of two paclitaxel-resistant high-grade serous carcinoma cell lines with increased expression of P-glycoprotein". Frontiers in Oncology 11 (2021): http://dx.doi.org/10.3389/fonc.2021.752127. 10.3389/fonc.2021.752127 Nunes, Mariana; Henriques Abreu, Miguel; Bartosch, Carla; Ricardo, Sara. "Recycling the purpose of old drugs to treat ovarian cancer". International Journal of Molecular Sciences 21 20 (2020): 7768-7768. https://doi.org/10.3390/ijms21207768. 10.3390/ijms21207768 Coelho, Ricardo; Ricardo, Sara; Amaral, Ana Luísa; Huang, Yen-Lin; Nunes, Mariana; Neves, José Pedro; Mendes, Nuno; et al. "Regulation of invasion and peritoneal dissemination of ovarian cancer by mesothelin manipulation". Oncogenesis 9 6 (2020): http://dx.doi.org/10.1038/s41389-020-00246-2. 10.1038/s41389-020-00246-2 Pinto, Cátia; Custódio, Valéria; Nunes, Mariana; Songy, Aurélie; Rabenoelina, Fanja; Courteaux, Barbara; Clément, Christophe; Gomes, Ana Catarina; Fontaine, Florence. "Understand the potential role of Aureobasidium pullulans, a resident microorganism from grapevine, to prevent the infection caused by Diplodia seriata". Frontiers in Microbiology 9 (2018): http://dx.doi.org/10.3389/fmicb.2018.03047. 10.3389/fmicb.2018.03047 Félix, Carina; Nunes, Mariana; Libório, Sofia; Félix, Rafael; Duarte, Ana Sofia; Alves, Artur; Esteves, Ana Cristina. "Lasiodiplodia theobromae as a producer of biotechnologically relevant enzymes". International Journal of Molecular Sciences 19 2 (2018): 29. http://dx.doi.org/10.3390/ijms19020029. 10.3390/ijms19020029
Newspaper article	Almeida-Nunes, Diana Luisa; Nunes, Mariana; Osorio, Hugo; Ferreira, Verónica; Lobo, Cláudia; Monteiro, Paula; Abreu, Miguel Henriques; et al. "Ovarian cancer ascites proteomic profile reflects metabolic changes during disease progression", Biochemistry and Biophysics Reports, 2024
Thesis / Dissertation	Nunes, Mariana. "First line drug testing in ovarian cancer cell lines manipulated for Mesothelin expression". Master, Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, 2019. https://repositorio-aberto.up.pt/handle/10216/122642.

Award

2022	Best Poster Award - Combining Paclitaxel With Repurposing Drugs Improve Ovarian Cancer Management APCF Associação Portuguesa Ciências Forenses, Portugal

Other distinction

2022	EACR-Worldwide Cancer Research Meeting Bursary - The invisible phase of metastasis: Impact of dormancy, cancer cell plasticity and evolution of the niche during metastatic progression - Virtual Event European Association For Cancer Research, United Kingdom