Hugo Miguel Raposo Correia Botelho (E218-E579-24C1)

Curriculum Vitae

Hugo Miguel Raposo Correia Botelho

Hugo M. Botelho is a Junior Researcher at the Biosystems & Integrative Sciences Institute (BioISI) of the Faculty of Sciences University of Lisbon (FCUL) and Manager of BioISI's High-Throughput Screening (HTS) Facility. Scientist with a strong expertise in microscopy, cell biology, bioimage analysis, drug discovery and protein folding in vivo and in vitro. Obtained his PhD in Biochemistry (specialty: biophysics) in 2010 under the supervision of Cláudio M. Gomes (ITQB/UNL) for studies on protein folding and aggregation. In 2012 joined Margarida Amaral's lab at FCUL to research novel therapeutics for Cystic Fibrosis (CF). For 1.5 years was trained on high-throughput microscopy, RNA interference screening and membrane trafficking at EMBL Heidelberg. Since 2014 he has been employing siRNA-based screens to characterize novel therapeutic CF target genes. As principal investigator he has coordinated research projects focusing on the identification and characterization of novel molecules with therapeutic relevance for CF using natural compound sources and HTS methodologies. Since 2014 he has established a HTS Facility at BioISI and now serves as its manager, which includes supervision of 1 research technician. Each year the facility provides 5000 hours of equipment access time to about 40 users regarding sample preparation, imaging, data analysis and screening assay services. He is also actively engaged in developing and deploying automated image analysis algorithms for high content microscopy, software development and data science approaches to biological data. Has authored 29 original peer reviewed publications, 4 book chapters, supervised students and post-docs and been involved in several scientific research projects, both as team member and principal investigator. As manager of the HTS Facility, represents BioISI and FCUL at the Portuguese Platform of BioImaging (PPBI), PT-OPENSCREEN, Euro-BioImaging ERIC, EU-OPENSCREEN, the Lisbon Area BioImaging Group, among others. He is a regular trainer and speaker at microscopy and CF meetings and courses. h-index 14 (Scopus, May 2023)

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Identification

Personal identification

Full name: Hugo Miguel Raposo Correia Botelho
Gender: Male

Citation names

Botelho, Hugo M

Author identifiers

Ciência ID: E218-E579-24C1
ORCID iD: 0000-0002-4208-1086
Google Scholar ID: nXftyYwAAAAJ&hl
Researcher Id: B-3092-2008
Scopus Author Id: 15030102200

Email addresses

hmbotelho@ciencias.ulisboa.pt (Professional)

Telephones

Telephone

217500659 (Professional)

Addresses

BioISI - Biosystems & Integrative Sciences Institute. Campo Grande, C8, 1749-016, Lisboa, Lisboa, Portugal (Professional)

Websites

http://webpages.fc.ul.pt/~hmbotelho (Scholar)
http://bioisi.pt/hts (Scholar)
https://www.researchgate.net/profile/Hugo_Botelho (Social media)
https://github.com/hmbotelho (Social media)
https://www.linkedin.com/in/hugo-botelho-b605b927 (Social media)

Knowledge fields

Natural sciences - Biological Sciences - Cell Biology
Exact Sciences - Computer and Information Sciences
Medical and Health Sciences - Medical Biotechnology
Natural sciences - Biological Sciences - Biophysics

Languages

Language	Speaking	Reading	Writing	Listening	Peer-review
English	Advanced (C1)	Advanced (C1)	Advanced (C1)	Advanced (C1)	Advanced (C1)
French	Beginner (A1)	Beginner (A1)	Beginner (A1)	Beginner (A1)	Beginner (A1)
Spanish; Castilian	Beginner (A1)	Beginner (A1)	Beginner (A1)	Beginner (A1)	Beginner (A1)
Portuguese (Mother tongue)

Education

	Degree	Classification
2010/11/22 Concluded	Biochemistry (Doutoramento) Major in Biophysics Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal "Metal Ions and Protein Folding - Conformational and Functional Interplay" (THESIS/DISSERTATION)	Approved by unanimity
2006/07/14 Concluded	Bioquímica (Licenciatura) Universidade de Lisboa Faculdade de Ciências, Portugal "Pesquisa, identificação e caracterização de proteínas hiperestáveis no proteoma solúvel da Archaea hipertermofílica Sulfurisphaera sp." (THESIS/DISSERTATION)	18

Affiliation

Science

	Category Host institution	Employer
2019/07/01 - Current	Contracted Researcher (Research)	Universidade de Lisboa Faculdade de Ciências, Portugal
	Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2015/05/01 - 2019/06/30	Postdoc (Research)	Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal

2012/04/15 - 2019/06/30	Postdoc (Research)	Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal

2012/07/16 - 2013/12/31	Visiting Researcher (Research)	European Molecular Biology Laboratory, Germany

2012 - 2013	Researcher (Research)	Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal

2011/09/01 - 2012/04/14	Postdoc (Research)	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

2011/07/03 - 2011/10/03	Postdoc (Research)	Universitätsklinikum Freiburg Neurologische Universitätsklinik, Germany

2010/11/23 - 2011/06/30	Postdoc (Research)	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

2011 - 2011	Researcher (Research)	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

2010/01/25 - 2010/02/07	Visiting Researcher (Research)	Umea Universitet, Sweden

2005/09/05 - 2006/07/14	Research Trainee (Research)	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Others

	Category Host institution	Employer
2006/10/01 - 2010/11/22	PhD Student	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Projects

Grant

	Designation	Funders
2016/05/01 - 2018/01/01	The identification of new natural compounds of high therapeutic potential for Cystic Fibrosis by high-throughput microscopy screens BioISI Post-Doc Supervisor Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal	Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas Concluded

Contract

	Designation	Funders
2024/01/01 - Current	ORGESTRA - Organoid technologies for disease modeling, drug discovery and development for rare diseases 101120108 Horizon Europe, Belgium	Ongoing
2022/02/24 - Current	ISIDORe - Integrated Services for Infectious Diseases Outbreak Research 101046133 Researcher Universidade de Lisboa Faculdade de Ciências, Portugal	European Union Ongoing
2024/02 - 2024/09	Biocompatible Coatings with Natural-Based Nano-Agents for Biomedical Applications BioISI Projects 2024 Co-Principal Investigator (Co-PI) Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2023/01/01 - 2024/06/30	NewKinCF - Unraveling the mechanism of action of a novel kinase regulator of F508del-CFTR traffic and activity 2022.03453.PTDC Principal investigator Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2022/05 - 2023/04	Mitochondrial network in Multiple Acyl-CoA Dehydrogenase Deficiency: construction of a high-content bioimage analysis workflow BioISI Projects 2022 Principal investigator Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2017/06/01 - 2021/10/31	PPBI - Portuguese Platform of BioImage PPBI-POCI-01-0145-FEDER-022122 Researcher Universidade de Lisboa Faculdade de Ciências, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2018/10/01 - 2021/09/30	Identificação de Novos Fármacos para Fibrose Quística PTDC/MED-QUI/28800/2017	Fundação para a Ciência e a Tecnologia Ongoing
2020/02/04 - 2021/03/31	VALHealth - Valorisation of Algae for Health: Bioactive Compounds from Marine Bioresources by Membrane Technology BioISI Projects 2020 Principal investigator Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal	Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas Concluded
2018 - 2021	AbrogateBM: Detect, prevent, erradicate: a multi-step strategy to abrogate brain metastasization of breast cancer PTDC/MED-ONC/29402/2017 Consultant Universidade de Lisboa Instituto de Investigação do Medicamento, Portugal	Associação para a Inovação e Desenvolvimento da FCT Concluded
2015/09/01 - 2020/05/31	CORBEL - Coordinated Research Infrastructures Building Enduring Life-science Services 654248 VIP User Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal	European Union Concluded
2019/01/01 - 2019/12/31	Instituto de Biosistemas & Ciências Integrativas UID/Multi/04046/2019 Instituto Nacional de Saúde Doutor Ricardo Jorge, Portugal Universidade do Minho, Portugal Universidade do Porto Faculdade de Ciências, Portugal Fundação da Faculdade de Ciências da Universidade de Lisboa, Portugal FCiênciasID Associação para a Investigação e Desenvolvimento de Ciências, Portugal Universidade de Lisboa Faculdade de Ciências, Portugal Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2016 - 2019	Novel CFTR Traffic Factors Related with Epithelial Cell Differentiation: Potential Therapeutic Targets for Cystic Fibrosis PTDC/BIM-MEC/2131/2014 Researcher Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2018/02/01 - 2018/12/31	Deconvolution of dual CFTR/ANO1 Modulators from Portuguese natural products - A new class of drugs for CF therapy BioISI Projects 2018 Principal investigator Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal	Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas Concluded
2016 - 2018	MIMED - Mining the molecular metric space for drug design PTDC/EEI-ESS/4923/2014 Researcher Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2017/01/07 - 2017/12/30	A new class of drugs for CF therapy - Dual CFTR/ANO1 Modulators from Portuguese natural products BioISI Projects 2017 Principal investigator Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal	Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas Concluded
2014 - 2017	Innovative non-CFTR approaches to cystic fibrosis therapy Strategic Research Centre Grant Researcher Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal	Cystic Fibrosis Trust Concluded
2016/01/05 - 2016/12/16	Natural compounds as a source of novel drug leads for Cystic Fibrosis BioISI Projects 2016 Principal investigator Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal	Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas Concluded
2011 - 2013	Identification of novel targets rescuing of F508del-CFTR traffic: Mechanism of action 7207534 Researcher Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal	Cystic Fibrosis Foundation Concluded
2011/01/01 - 2012/12/31	Strategic Project - LA 4 - 2011-2012 PEst-OE/EQB/LA0004/2011 Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica António Xavier, Portugal Fundação Calouste Gulbenkian, Portugal Instituto de Biologia Experimental e Tecnológica, Portugal Universidade NOVA de Lisboa NOVA Medical School, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2012/02/01 - 2012/04/15	Mechanisms of SOD1 toxic aggregation in neurodegenerative processes info:eu-repo/grantAgreement/FCT/3599-PPCDT/117789/PT Researcher Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2011 - 2012	Amyloidogenesis of the S100A8/A9 cytokine as an anti-proliferative mechanism in prostate cancer Terry Fox Researcher Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal	Liga Portuguesa Contra o Cancro Concluded
2008/01/02 - 2011/12/31	Towards the understanding of the energetic and structural interplay between metal sites and protein folding info:eu-repo/grantAgreement/FCT/3599-PPCDT/70101/PT Researcher Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2007/07/01 - 2011/06/30	Functional analysis and origins of the pathophysiology of mutations within genes of the fatty acid oxidation pathway: implications in the multiple acyl-CoA dehydrogenase deficiency disorder info:eu-repo/grantAgreement/FCT/3599-PPCDT/70033/PT Researcher Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal	Fundação para a Ciência e a Tecnologia Concluded

Outputs

Publications

Book chapter	Amaral, Margarida; Clarke, Luka A; Carlos M. Farinha; Botelho, Hugo. "Systems Biology and the New Omics". In Hodson and Geddes' Cystic Fibrosis, edited by Andrew Bush; Margarida D. Amaral; Jane C. Davies; Nicholas J. Simmonds; Jennifer L. Taylor-Cousar; Sarath C. Ranganathan, 96-110. Boca Raton, FL, United States: Taylor & Francis. CRC Press, 2023. Published • 10.1201/9781003262763 Carvalho, Sofia B.; Cardoso, Isabel; Botelho, Hugo M; Yanamandra, Kiran; Fritz, Günter; Gomes, Cláudio M.; Morozova-Roche, Ludmilla A.. "Structural Heterogeneity and Bioimaging of S100 Amyloid Assemblies". In Bio-nanoimaging, 197-212. Elsevier, 2014. Published • 10.1016/b978-0-12-394431-3.00018-3 Botelho, Hugo M; Fritz, Günter; Gomes, Cláudio M.. "Analysis of S100 Oligomers and Amyloids". In Methods in Molecular Biology, 373-386. Humana Press, 2012. 10.1007/978-1-61779-551-0_25
Conference abstract	Marques, Rute S.; Ana Rita Garcia; Godinho-Pereira, Joana; Malhó, Rui; Botelho, Hugo M; Brito, Maria. "Targeting Cyclin D1/CDK4/6 and PI3K pathways for the treatment of metastatic breast cancer.". Paper presented in Fundação AstraZeneca Innovate Competition, iMed Conference® Lisbon 2023, Lisbon, 2023. Submitted Dionisio Lopes, Margarida; Godinho-Pereira, Joana; Figueira, Inês; Botelho, Hugo M; Malhó, Rui; Maria Alexandra de Oliveira Silva Braga Pedreira de Brito. "Screening de novos e antigos fármacos: um modulador para a prevenção de metástases ccerebrais do cancro da mama.". Paper presented in 2º Encontro Nacional de Jovens Investigadores em Oncologia, Porto, 2020.
Conference poster	Daniela Neugebauer; Mariana Caleiro; Vasco Cachatra; Moiteiro, Cristina; Amaral, Margarida; Botelho, Hugo M. "High content screening for the characterization of the GRK5-dependent p.Phe508del-CFTR rescue in cystic fibrosis". Paper presented in Spanish & Portuguese Advanced Optical Microscopy Meeting – SPAOM2023, 2023. Daniela Neugebauer; Vasco Cachatra; Moiteiro, Cristina; Amaral, Margarida; Botelho, Hugo M. "Identification of genes that restore p.Phe508del-CFTR traffic in cystic fibrosis models through GRK5 inhibition". Paper presented in Chem&BioChem – Postgraduate Students Meeting, DQB/FCUL, 2023. Marques, Rute; Ana Rita Garcia; Godinho-Pereira, Joana; Malhó, Rui; Botelho, Hugo M; Maria Alexandra de Oliveira Silva Braga Pedreira de Brito. "Cyclin D1-CDK4/6 and PI3K signalling pathways as targets to combat metastatic triple negative and luminal A breast cancer". Paper presented in Jornadas Científicas 2023 Universidade de Lisboa, 2023. Marques, Rute; Ana Rita Garcia; Godinho-Pereira, Joana; Malhó, Rui; Botelho, Hugo M; Maria Alexandra de Oliveira Silva Braga Pedreira de Brito. "Exploiting cyclin D1/CDK4/6 and/or PI3K pathways to fight metastatic triple negative and luminal A breast cancer". Paper presented in 14th iMed.ULisboa Postgraduate Students Meeting, 2023. Fernandes AA; Martinho, Paulo N.; Bento, Marcos; Botelho, Hugo. "Thermo- and Photo-responsive Spin Labile Fe(III) complexes". Paper presented in CQE Days 2023, 2023. Pinto S; DUARTE, Ana Patrícia; Botelho, Hugo; Gaspar, Maria Manuela; Ascenção L; Faísca P; Catarina Pinto Reis; Pacheco, Rita. "Albumin Nanoparticles of Natural Products: In Vitro and In Vivo Evaluation". Paper presented in 4th SPLC-CRS Young Scientists Meeting, 2023. Coelho M; DUARTE, Ana Patrícia; Botelho, Hugo; Catarina Pinto Reis; Maria Luísa Serralheiro; Pacheco, Rita. "Edible seaweeds extracts characterization and prospective health effects". Paper presented in 1st International Congress on Food, Nutrition & Public Health, 2022. Ana Rita Garcia; Godinho-Pereira, Joana; Figueira, Inês; Botelho, Hugo; Malhó, Rui; Brito, Alexandra. "Detalhando as alterações fenotípicas e morfológicas subjacentes ao extravasamento das células de cancro da mama através da barreira hematoencefálica". Paper presented in 4º Encontro Nacional de Jovens Investigadores em Oncologia, 2022. M. A. Bento; Paulo Nuno Martinho; Botelho, Hugo M; Diogo, Hermínio P.; Liliana P. Ferreira; Vítor H. N. Rodrigues. Corresponding author: M. A. Bento. "Fe(III) complexes as molecular magnetic actuators". Paper presented in MagnetismIn, 2022. Filipa S. Carvalho; Vukosava M Torres; Joana V. Ribeiro; Botelho, Hugo; Vilarinho, Laura; Cláudio M. Gomes; Henriques, Bárbara J. "Mitochondria phenotype and metabolomic signature in MADD patient-derived fibroblasts". Paper presented in SSIEM 2022 Annual Symposium, 2022. Marcos A. Bento; Botelho, Hugo; Ferreira, L.P.; Martinho, Paulo N.. "Molecular magnetic actuators based on Fe(III) complexes". Paper presented in EuChemS Chemistry Congress, 2022. Carvalho FS; Ribeiro JV; Botelho, Hugo; Vilarinho L; Gomes CM; Henriques, BJ. "Cellular studies to evaluate mitochondria function on MADD, a rare metabolic disorder". Paper presented in The Biochemistry Global Summit (25th IUBMB Congress, 46th FEBS Congress and 15th PABMB Congress), 2022. Ana Rita Garcia; Godinho-Pereira, Joana; Figueira, Inês; Botelho, Hugo M; Malhó, Rui; Brito, Maria Alexandra. "Dissecting the extravasation of triple-negative breast cancer cells across the blood-brain barrier: the landscape for brain metastasis prevention". Paper presented in 13th iMed.ULisboa Postgraduate Students Meeting, 2022. Ana Rita Garcia; Godinho-Pereira, Joana; Figueira, Inês; Botelho, Hugo M; Malhó, Rui; Brito, Maria Alexandra. "Molecular characterization and modulation of blood-brain barrier and breast cancer cells interaction: a step closer to brain metastasis prevention". Paper presented in EACR 2022 Congress ¿ Innovative Cancer Science: Translating Biology to Medicine, 2022. Ana Rita Garcia; Godinho-Pereira, Joana; Figueira, Inês; Botelho, Hugo M; Malhó, Rui; Brito, Maria Alexandra. "Understanding the blood-brain barrier and breast cancer cells' interaction: a step closer to brain metastasis prevention". Paper presented in Brain Tumor Meeting 2022, 2022. Godinho-Pereira, Joana; Lopes, Margarida Dionísio; Ana Rita Garcia; Figueira, Inês; Botelho, Hugo M; Malhó, Rui; Brito, Maria Alexandra. "Tackling breast cancer brain metastasis: a drug screening for a preventive approach to boost blood-brain barrier properties". Paper presented in Brain Tumor Meeting 2022, 2022. Ana Rita Garcia; Godinho-Pereira, Joana; Figueira, Inês; Botelho, Hugo M; Malhó, Rui; Brito, Maria Alexandra. "Emerging events in breast cancer cells extravasation through blood-brain barrier: the key for brain metastasis prevention". Paper presented in Encontro Ciência 2022, 2022. Ferreira, JF; Silva, Iris AL; Botelho, Hugo M; Amaral, Margarida; Farinha, CM. "Exploring the role of EPAC1 to stabilize CFTR in intestinal cells". Paper presented in 17th ECFS Basic Science Conference, 2022. Botelho, Hugo M; Lopes-Pacheco, Miquéias; Pinto, Madalena C.; Silva, Iris AL; Clarke, Luka; Cachatra, V; Neumann, B; et al. "New kinase therapeutic targets for Cystic Fibrosis from a global functional genomics screen". Paper presented in 17th ECFS Basic Science Conference, 2022. Botelho, Hugo M; Lopes-Pacheco, Miquéias; Pinto, Madalena C.; Silva, Iris AL; Clarke, Luka A; Cachatra, V; Neumann, B; et al. "New kinase therapeutic targets for Cystic Fibrosis identified through high-content functional genomics screens". Paper presented in 5ª Conferência Anual da redeSAÚDE, 2021. Ana Rita Garcia; Godinho-Pereira, Joana; Figueira, Inês; Kim, KS; Botelho, Hugo M; Malhó, Rui; Brito, MA. "Spatio-temporal and molecular characterization of breast cancer cells extravasation through blood-brain-barrier". Paper presented in iMed.ULisboa Meeting 2021, 2021. Ana Rita Garcia; Godinho-Pereira, Joana; Figueira, Inês; Kim, KS; Botelho, Hugo M; Malhó, Rui; Maria Alexandra de Oliveira Silva Braga Pedreira de Brito. "Disclosing molecular alterations in the blood-brain barrier endothelium during interaction with breast cancer cells: a step forward brain metastasis prevention.". Paper presented in Encontro de Ciência 2021, 2021. Ana Rita Garcia; Godinho-Pereira, Joana; Figueira, Inês; Kim, KS; Botelho, Hugo M; Malhó, Rui; Brito, MA. "Unravelling the extravasation of breast cancer cells through blood-brain barrier endothelial cells". Paper presented in 27th Porto Cancer Meeting, 2021. Ana Rita Garcia; Godinho-Pereira, Joana; Figueira, Inês; Kim, KS; Botelho, Hugo M; Malhó, Rui; Maria Alexandra de Oliveira Silva Braga Pedreira de Brito. "Unravelling the extravasation of breast cancer cells though blood-brain barrier endothelial cells.". Paper presented in Porto Cancer Meeting 2021, 2021. Dionisio Lopes, Margarida; Godinho-Pereira, Joana; Ana Rita Garcia; Figueira, Inês; Botelho, Hugo M; Malhó, Rui; Maria Alexandra de Oliveira Silva Braga Pedreira de Brito. "High-throughput screening of new and old drugs: a modulator to prevent breast cancer brain metastasis". Paper presented in Aims Meeting, 2021. Ana Rita Garcia; Joana Godinho-Pereira; Ines Figueira; KW Kim; Botelho, Hugo M; Rui Malhó; Maria Alexandra Brito. "Desvendando a interação entre as células de cancro da mama e as células endoteliais da barreira hematoencefálica ao longo do extravasamento". Paper presented in 2º Encontro Nacional de Jovens Investigadores em Oncologia, 2020. Garcia, R; Godinho-Pereira, J; Figueira, Inês; Kim, KS; Botelho, Hugo M; Malhó, R; Brito, MA. "Breast cancer cells extravasation into the brain: a dynamic trafficking across blood-brain barrier endothelium". Paper presented in 5th Annual Meeting of the Mind-Brain College of the University of Lisbon, 2019. Ramalho, AS; Vonk, AM; Silva, Iris AL; Botelho, Hugo M; Bor, R; van Mourik, P; Michel, S; et al. "High reproducibility of forskolin-induced swelling of intestinal organoids across three academic laboratories". Paper presented in 33rd Annual North American Cystic Fibrosis Conference, 2019. Garcia, R; Godinho-Pereira, J; Figueira, Inês; Cascão, R; Barata, J; Kim, KS; Marques, Luis; et al. "Interplay between breast cancer cells and blood-brain barrier endothelial cells along extravasation". Paper presented in 11th iMed.ULisboa Postgraduate Students Meeting and 4th i3DU Meeting, 2019. van Mourik, P; Vonk AM; Ramalho AS; Silva IAL; Botelho, Hugo; Bor R; Aguilera B; et al. "High reproducibility of forskolin-induced swelling of intestinal organoids across three academic laboratories". Paper presented in 42nd European Cystic Fibrosis Conference, 2019. Vonk, Annelotte M; Ramalho, Anabela S; Silva, Iris AL; Silva, Iris AL; Botelho, Hugo M; van Mourik, Peter; Michel, S; et al. "High reproducibility of forskolin-induced swelling of organoids across three academic laboratories.". Paper presented in 16th ECFS Basic Science Conference 2019, Dubrovnik, Croatia, 27-30 March 2019., 2019. Botelho, Hugo M; Halavatyi, A; Tischer, C. "shinyHTM: an open-source web application for the analysis of high throughput microscopy data". Paper presented in EMBL Conference: From Images to Knowledge with ImageJ & Friends (I2K), 2018. Astapenko, D; Botelho, Hugo M; Kolodkin, A; Westerhoff, HV; Amaral, Margarida. "Dynamic modelling of CFTR channel maturation in Cystic Fibrosis: what controls the channel concentration in the plasma membrane?". Paper presented in The 11th International Conference on Bioinformatics of Genome Regulation and Structure\Systems Biology, 2018. Quaresma, MC; Botelho, Hugo M; Pankonien, I; Pepperkok, R; Amaral, Margarida. "Role of CFTR in epithelial-mesenchymal transition (EMT) by functional genomics". Paper presented in Encontro Ciência 2018, 2018. Lopes-Pacheco, M; Botelho, Hugo M; Amaral, Margarida. "Novel F508del-CFTR traffic correctors among FDA-approved drugs and mechanism of action of RDR01752 corrector". Paper presented in EMBO Workshop: Proteostasis, 2017. Botelho, Hugo M; Lim, SH; Tischer, C; Pepperkok, R; Stagljar, I; Amaral, Margarida. "Validation and classification of genes releasing misfolded F508del-CFTR from the endoplasmic reticulum". Paper presented in EMBO Workshop: Proteostasis, 2017. Awatade, NT; Felício, V; Ramalho, S; Botelho, Hugo M; Silva, Iris AL; de Poel, E; Vonk, AM; et al. "Functional and biochemical assessment of correctors and potentiators on the rare Cystic Fibrosis-causing R560S mutation". Paper presented in 31st Annual North American Cystic Fibrosis Conference, 2017. Pinto, MC; Lérias, JR; Botelho, Hugo M; Awatade, NT; Pepperkok, R; Kunzelmann, K; Amaral, Margarida. "Anoctamin 1 – Identification of novel regulators and their significance to Cystic Fibrosis". Paper presented in 31st Annual North American Cystic Fibrosis Conference, 2017. Cristóvão, JS; Morris, V; Cardoso, Isabel; Leal, SS; Botelho, Hugo M; Göbl, C; Kierdorf, K; et al. "The neuronal S100B cytokine targeting amyloid-ß aggregation in Alzheimer’s Disease". Paper presented in 42nd FEBS Congress, 2017. Canato, S; Carvalho, AS; Botelho, Hugo M; Aloria, K; Matthiesen, Rune; Amaral, Margarida; Falcão, AO; Farinha, CM. "Modulation of protein traffic networks to rescue F508del-CFTR from the endoplasmic reticulum". Paper presented in 40th European Cystic Fibrosis Conference, 2017. Baptista, C; Botelho, Hugo M; Tenreiro, RP; Awatade, NT; Amaral, Margarida; Vieira, H. "From Portuguese natural heritage to Cystic Fibrosis therapies – a story of nature and technology for pharmaceutical applications". Paper presented in 3rd Annual Conference Rede Mar, 2017. Cristóvão, J; Morris, V; Cardoso, Isabel; Leal, SS; Botelho, Hugo M; Göbl, C; Kierdorf, K; et al. "Role of the S100B pro-inflammatory cytokine implicated in aging and Alzheimer’s Disease as a calcium-regulated chaperone acting on amyloid-ß aggregation". Paper presented in 4th Annual Conference Rede Saúde, 2017. Quaresma, MC; Botelho, Hugo M; Amaral, Margarida. "Role of CFTR in the maintenance of airway epithelial cell differentiation". Paper presented in 14th ECFS Basic Science Conference, 2017. Pinto, MC; Lérias, JR; Botelho, Hugo M; Awatade, NT; Pepperkok, P; Kunzelmann, K; Amaral, Margarida. "Anoctamins 1 and 6 – Identification of novel traffic regulators and their significance to Cystic Fibrosis". Paper presented in 14th ECFS Basic Science Conference, 2017. Lérias, JR; Pinto, MC; Botelho, Hugo M; Schreiber, R; Amaral, Margarida; Kunzelmann, K. "Anoctamin 1 traffic and function is enhanced by tethering proteins family of extended synaptotagmins". Paper presented in 14th ECFS Basic Science Conference, 2017. Igreja, S; Botelho, Hugo M; Felício, V; Pagani, G; Pagani, M; Amaral, Margarida. "Validation of a CFTR alternative splicing reporter system for high-throughput microscopy and identification of novel CFTR regulators of alternative splicing". Paper presented in 14th ECFS Basic Science Conference, 2017. Awatade, NT; Botelho, Hugo M; Felício, V; Clarke, LA; Silva, Iris AL; Marques, Luis; Ramos, M; Amaral, Margarida. "CFTR modulators enhance function R334W-CFTR both in intestinal organoids and conditionally reprogrammed human nasal epithelial cells". Paper presented in 14th ECFS Basic Science Conference, 2017. Felício, V; Botelho, Hugo M; Igreja, S; Amaral, Margarida. "A high-throughput screening assay to identify factors correcting CFTR mutations bearing premature termination codons (PTCs)". Paper presented in 14th ECFS Basic Science Conference, 2017. Baptista, C; Vieira, H; Tenreiro, R; Awatade, NT; Amaral, Margarida; Botelho, Hugo M. "High throughput microscopy screens identify F508del-CFTR correctors and ANO1 traffic modulators in a unique Portuguese natural compound library". Paper presented in 14th ECFS Basic Science Conference, 2017. Botelho, Hugo M; Lim, SH; Tischer, C; Pepperkok, R; Stagljar, I; Amaral, Margarida. "A high content siRNA screen at near genomic scale reveals novel F508del-CFTR therapeutic targets". Paper presented in 14th ECFS Basic Science Conference, 2017. Botelho, Hugo M; Awatade, NT; Tischer, C; Amaral, MD. "Modular high content image analysis workflows leveraging biology and biomedicine". Paper presented in NEUBIAS2020 BioImage Analysis Symposium, 2017. Botelho, Hugo M; Quaresma, MC; Amaral, Margarida. "CFTR in the maintenance of epithelial differentiation in the airways". Paper presented in Chem&BioChem Postgraduate Students Meeting, 2017. Igreja, S; Botelho, Hugo M; Amaral, Margarida. "Identification of novel regulators of alternative splicing by HT microscopy: implications for Cystic Fibrosis and other human diseases". Paper presented in EMBO Practical Course: High-Throughput Microscopy for Systems Biology, 2016. Pankonien, I; Santos-Sousa, L; Botelho, Hugo M; Amaral, Margarida. "Establishment of cell-based assays of airway epithelial differentiation and wound healing for automated microscopy". Paper presented in 13th ECFS Basic Science Conference, 2016. Lérias, JR; Afonso, Sara; Quaresma, MC; Pinto, M; Awatade, NT; Botelho, Hugo M; Schreiber, R; et al. "Anoctamin 1 - A member of a novel family of ion channels with extended functions and significance in disease". Paper presented in 13th ECFS Basic Science Conference, 2016. Awatade, NT; Botelho, Hugo M; Felício, V; Clarke, LA; Ramos, M; Amaral, Margarida. "To assess the efficacy of CFTR modulators on rare Cystic Fibrosis mutations using forskolin-induced swelling assay in human intestinal organoids". Paper presented in 13th ECFS Basic Science Conference, 2016. Awatade, NT; Botelho, Hugo M; Felício, V; Clarke, LA; Ramos, M; Amaral, Margarida. "Assessment of CFTR activity ex vivo as a basis for personalised medicine". Paper presented in 5th Workshop Structure and Function of Ion Channels and Transporters, 2015. Pankonien, I; Afonso, Sara; Quaresma, MC; Kmit, A; Dahimène, S; Botelho, Hugo M. "The epithelial transcription factor KLF4 affects the traffic of CFTR". Paper presented in 12th ECFS Basic Science Conference, 2015. Igreja, S; Botelho, Hugo M; Marques, Luis; Amaral, Margarida. "In vitro correction of the CFTR 2657+5G>A splicing mutation by an antisense oligonucleotide". Paper presented in 12th ECFS Basic Science Conference, 2015. Botelho, Hugo M; Dahimène, S; Uliyakina, I; Awatade, NT; Proença, MC; Neumann, B; Tischer, C; et al. "Rescuing F508del-CFTR in airway epithelial cells using a novel traffic-based high content microscopy screening platform". Paper presented in 12th ECFS Basic Science Conference, 2015. Botelho, Hugo M; Dahimène, S; Uliyakina, I; Neumann, B; Tischer, C; Pepperkok, R; Amaral, Margarida. "A high content screening approach for the identification of CFTR traffic regulators in airway epithelial cells". Paper presented in ESF/EMBO Cell Polarity and Membrane Traffickin, 2014. Carvalho, SB; Botelho, Hugo M; Leal, SS; Cardoso, Isabel; Fritz, G; Gomes, Cláudio M. "Intrinsically disordered and aggregation prone regions underlie ß-aggregation in S100 proteins". Paper presented in 2nd Iberian Congress on Prions, 2013. Botelho, Hugo M; Dahimène, S; Uliyakina, I; Neumann, B; Tischer, C; Pepperkok, R; Amaral, Margarida. "High throughput screen reveals genes regulating wt-CFTR and rescuing F508del-CFTR traffic". Paper presented in 27th Annual North American Cystic Fibrosis Conference, 2013. Botelho, Hugo M; Dahimène, S; Uliyakina, I; Neumann, B; Tischer, C; Pepperkok, R; Amaral, Margarida. "Identification of CFTR traffic regulators as a source of novel Cystic Fibrosis therapeutic targets". Paper presented in The 5th EMBO Meeting, 2013. Sofia B Carvalho; Sónia S. Leal; Botelho, Hugo M; Isabel Cardoso; Günter Fritz; Cláudio M. Gomes. "Cross talks between amyloid-forming proteins in neurodegeneration". Paper presented in 9th EBSA European Biophysics Congress, 2013. Botelho, Hugo; Dahimène S; Uliyakina I; Neumann B; Tischer C; Pepperkok R; Amaral MD. "High throughput screening as a source for novel cystic fibrosis therapeutic targets". Paper presented in 36th European Cystic Fibrosis Conference, 2013. Dahimène S; Romão A; Botelho, Hugo; Palma M; Correia AR; Farinha CM; King-Underwood J; et al. "WS21.9 Screening for novel F508del-CFTR correctors identified by pharmacophore modelling through automated microscopy and NBD1 folding assays". Paper presented in 36th European Cystic Fibrosis Conference, 2013. Bandeiras, T; Freitas, MC; Petrasch, D; Botelho, Hugo M; Gomes, Cláudio M; Kletzin, A; Frazão, Carlos. "Purification, crystallization and preliminary crystallographic analysis of Acidianus ambivalens Rieske ferredoxin 2 (RFd2), a new type of Rieske ferredoxins with an additional disulfide". Paper presented in 1° Encontro Nacional de Utilizadores de Radiação de Sincrotrão, 2012. Botelho, Hugo M; Christine Betz; Katrin Kierdorf; Marco Prinz; Cláudio M. Gomes; Günter Fritz. "A new emerging role of S100B in amyloid plaque formation". Paper presented in 56th Annual Meeting, German Society for Neuropathology and Neuroanatomy, 2011. 10.5414/npp30245 Bandeiras, T; Freitas, MC; Botelho, Hugo M; Gomes, Cláudio M; Carrondo, MA; Kletzin, A; Frazão, Carlos. "Crystallographic structure of Acidianus ambivalens Rieske ferredoxin 2 – The disulfide bridge structural role". Paper presented in EMBO Practical Course: High-throughput methods for Protein Production and Crystallization, 2011. Botelho, Hugo M; Yanamandra, K; Fritz, G; Morozova-Roche, LA; Gomes, Cláudio M. "S100 proteins: modulators of Aß amyloidogenesis". Paper presented in Interbio Symposium 'Frontiers in Protein Research', 2011. Gomes, Cláudio M; Botelho, Hugo M; Yanamandra, K; Fritz, G; Morozova-Roche, LA. "Metal ions and amyloid-beta peptide fibrils as modulators of S100 amyloidogenesis". Paper presented in FASEB Summer Research Conference - Protein Folding in the Cell, 2010. Botelho, Hugo M; Yanamandra, K; Fritz, G; Morozova-Roche, LA; Gomes, Cláudio M. "Metal ions and Aß fibrils modulate S100 amyloidogenesis". Paper presented in IV Spanish-Portuguese Biophysics Congress, 2010. Almeida, M; Magalhães, LC; Sabino, FMR; Botelho, Hugo M; Gomes, Cláudio M. "Focused NMR structural proteomics on hyperstable proteome subsets". Paper presented in EUROMAR 2010, 2010. Botelho, Hugo M; Koch, M; Fritz, G; Gomes, Cláudio M. "Metal ions as modulators of folding and stability of the S100A2 tumor suppressor". Paper presented in Gordon Research Seminar & Gordon Research Conference “Protein Folding Dynamics”, 2010. Botelho, Hugo M; Koch, M; Fritz, G; Gomes, Cláudio M. "Calcium and zinc modulate the folding and stability of the S100A2 tumor suppressor". Paper presented in VIII European Symposium of The Protein Society, 2009. Botelho, Hugo M; Koch, M; Fritz, G; Gomes, Cláudio M. "Ca2+ and Zn2+ modulate the conformation and stability of the S100A2 tumor suppressor". Paper presented in The 9th European Biological Inorganic Chemistry Conference, EUROBIC9, 2008. Botelho, Hugo M; Koch, M; Fritz, G; Gomes, Cláudio M. "Role of Ca2+ and Zn2+ as modulators of the S100A2 tumor suppressor". Paper presented in FASEB Summer Research Conference – Protein Folding in the Cell, 2008. Nascimento, C; Botelho, Hugo M; Tavares de Almeida, I; Gomes, Cláudio M; Leandro P. "Folding rescue of mild PKU mutations by chemical chaperones". Paper presented in Society for the Study of Inborn Errors of Metabolism Symposium 2007, SSIEM Symposium 2007, 2007. Botelho, Hugo M; Koch, M; Fritz, G; Gomes, Cláudio M. "Conformational control of S100A2 by metal ions". Paper presented in 13th International conference on Biological Inorganic Chemistry, ICBIC XIII, 2007. Prosinecki, V; Botelho, Hugo M; Gomes, Cláudio M. "A proteomic approach to investigate protein stability". Paper presented in VII European Symposium of the Protein Society – From Proteins to Proteome, 2007. Botelho, Hugo M; Koch, M; Fritz, G; Gomes, Cláudio M. "Studies on the conformational effects of calcium and zinc binding to S100A2". Paper presented in VII European Symposium of the Protein Society – From Proteins to Proteome, 2007. Prosinecki, V; Botelho, Hugo M; Francese, S; Mastrobuoni, G; Moneti, G; Urich, T; Kletzin, A; Gomes, Cláudio M. "Mining for proteins with enhanced intrinsic thermal stability: a proteomic research on hyperthermophile Sulfurisphaera sp.". Paper presented in International Conference on Proteomics, PROTEOMLUX, 2006. Francese, S; Prosinecki, V; Botelho, Hugo M; Mastrobuoni, G; Pieraccini, G; Kletzin, A; Gomes, Cláudio M. "Proteomic characterization of hyperstable proteins within the soluble proteome of Sulfurisphaera sp.". Paper presented in 17th International Mass spectrometry Conference, 2006. Prosinecki, V; Botelho, Hugo M; Francese, S; Kletzin, A; Gomes, Cláudio M. "Ferredoxin and oxidative stress defence proteins are among the most stable proteins in the thermophile Sulfurisphaera sp.: a proteomic study". Paper presented in EUROBIC Young Researchers Forum and The 8th European Biological Chemistry Conference, EUROBIC8, 2006.
Journal article	Mariana Coelho; Ana Patrícia Duarte; Sofia Pinto; Hugo M. Botelho; Catarina Pinto Reis; Maria Luísa Serralheiro; Rita Pacheco. "Edible Seaweeds Extracts: Characterization and Functional Properties for Health Conditions". Antioxidants 12 3 (2023): 684. https://doi.org/10.3390/antiox12030684. Published • 10.3390/antiox12030684 Margarida C Quaresma; Hugo M Botelho; Ines Pankonien; Cláudia S Rodrigues; Madalena C Pinto; Pau R Costa; Aires Duarte; Margarida D Amaral. "Exploring YAP1-centered networks linking dysfunctional CFTR to epithelial–mesenchymal transition". Life Science Alliance (2022): https://doi.org/10.26508/lsa.202101326. 10.26508/lsa.202101326 Joana Godinho-Pereira; Margarida Dionísio Lopes; Ana Rita Garcia; Hugo M. Botelho; Rui Malhó; Inês Figueira; Maria Alexandra Brito. "A Drug Screening Reveals Minocycline Hydrochloride as a Therapeutic Option to Prevent Breast Cancer Cells Extravasation across the Blood–Brain Barrier". Biomedicines (2022): https://doi.org/10.3390/biomedicines10081988. 10.3390/biomedicines10081988 João F. Ferreira; Iris A. L. Silva; Hugo M. Botelho; Margarida D. Amaral; Carlos M. Farinha. "Absence of EPAC1 Signaling to Stabilize CFTR in Intestinal Organoids". Cells (2022): https://doi.org/10.3390/cells11152295. 10.3390/cells11152295 Célia Fernandes; Elisa Palma; Francisco Silva; Ana Belchior; Catarina I. G. Pinto; Joana F. Guerreiro; Hugo M. Botelho; et al. "Searching for a Paradigm Shift in Auger-Electron Cancer Therapy with Tumor-Specific Radiopeptides Targeting the Mitochondria and/or the Cell Nucleus". International Journal of Molecular Sciences (2022): https://doi.org/10.3390/ijms23137238. 10.3390/ijms23137238 Pinto, Madalena C.; Botelho, Hugo M; Silva, Iris AL; Violeta Railean; Beate Neumann; Rainer Pepperkok; Rainer Schreiber; Karl Kunzelmann; Amaral, Margarida. "Systems Approaches to Unravel Molecular Function: High-content siRNA Screen Identifies TMEM16A Traffic Regulators as Potential Drug Targets for Cystic Fibrosis". Journal of Molecular Biology 434 5 (2022): 167436-167436. http://dx.doi.org/10.1016/j.jmb.2021.167436. Published • 10.1016/j.jmb.2021.167436 Sang Hyun Lim; Jamie Snider; Liron Birimberg-Schwartz; Wan Ip; Joana C Serralha; Botelho, Hugo M; Miquéias Lopes-Pacheco; et al. "CFTR interactome mapping using the mammalian membrane two-hybrid high-throughput screening system". Molecular Systems Biology 18 2 (2022): https://doi.org/10.15252/msb.202110629. 10.15252/msb.202110629 Marne C Hagemeijer; Annelotte M Vonk; Nikhil T Awatade; Iris A L Silva; Christian Tischer; Volker Hilsenstein; Jeffrey M Beekman; et al. Corresponding author: Hugo M Botelho. "An open-source high-content analysis workflow for CFTR function measurements using the forskolin-induced swelling assay". Bioinformatics (2020): https://doi.org/10.1093/bioinformatics/btaa1073. 10.1093/bioinformatics/btaa1073 Silva IAL; Doušová T; Ramalho S; Centeio R; Clarke LA; Railean V; Botelho, Hugo M; et al. "Organoids as a personalized medicine tool for ultra-rare mutations in cystic fibrosis: The case of S955P and 1717-2A>G.". Biochimica et biophysica acta. Molecular basis of disease 1866 (2020): 165905. http://europepmc.org/abstract/med/32730979. Published • 10.1016/j.bbadis.2020.165905 Inna Uliyakina; Botelho, Hugo M; Ana C. da Paula; Sara Afonso; Miguel J. Lobo; Verónica Felício; Carlos M. Farinha; Margarida D. Amaral. "Full Rescue of F508del-CFTR Processing and Function by CFTR Modulators Can Be Achieved by Removal of Two Regulatory Regions". International Journal of Molecular Sciences 21 12 (2020): 4524-4524. https://doi.org/10.3390/ijms21124524. Published • 10.3390/ijms21124524 Amaral, Margarida D.; Hutt, Darren M; Tomati, Valeria; Botelho, Hugo M; Pedemonte, Nicoletta. "CFTR processing, trafficking and interactions". Journal of Cystic Fibrosis 19 (2020): S33-S36. http://dx.doi.org/10.1016/j.jcf.2019.10.017. Published • 10.1016/j.jcf.2019.10.017 João D. Santos; Sara Canato; Ana S. Carvalho; Botelho, Hugo M; Kerman Aloria; MD Amaral; Rune Matthiesen; Andre O. Falcao; Carlos Farinha. "Folding Status Is Determinant over Traffic-Competence in Defining CFTR Interactors in the Endoplasmic Reticulum". Cells 8 4 (2019): 353. https://www.mdpi.com/2073-4409/8/4/353. Published • 10.3390/cells8040353 Awatade, Nikhil T.; Ramalho, Sofia; Silva, Iris A.L.; Felício, Verónica; Botelho, Hugo M; de Poel, Eyleen; Vonk, Annelotte; et al. "R560S: A class II CFTR mutation that is not rescued by current modulators". Journal of Cystic Fibrosis 18 2 (2019): 182-189. http://dx.doi.org/10.1016/j.jcf.2018.07.001. Published • 10.1016/j.jcf.2018.07.001 Palma, Elisa; Botelho, Hugo M; Morais, Goreti Ribeiro; Rodrigues, Inês; Santos, Isabel Cordeiro; Campello, Maria Paula Cabral; Raposinho, Paula; et al. "Unravelling the antitumoral potential of novel bis(thiosemicarbazonato) Zn(II) complexes: structural and cellular studies". JBIC Journal of Biological Inorganic Chemistry 24 1 (2018): 71-89. http://dx.doi.org/10.1007/s00775-018-1629-6. Published • 10.1007/s00775-018-1629-6 Joana S. Cristóvão; Vanessa K. Morris; Isabel Cardoso; Sónia S. Leal; Javier Martínez; Botelho, Hugo M; Christoph Göbl; et al. "The neuronal S100B protein is a calcium-tuned suppressor of amyloid-ß aggregation". Science Advances 4 6 (2018): eaaq1702-eaaq1702. https://doi.org/10.1126/sciadv.aaq1702. Published • 10.1126/sciadv.aaq1702 Lérias, Joana R.; Pinto, Madalena C.; Botelho, Hugo M; Awatade, Nikhil T.; Quaresma, Margarida C.; Silva, Iris A.L.; Wanitchakool, Podchanart; et al. "A novel microscopy-based assay identifies extended synaptotagmin-1 (ESYT1) as a positive regulator of anoctamin 1 traffic". Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 1865 2 (2018): 421-431. http://dx.doi.org/10.1016/j.bbamcr.2017.11.009. Published • 10.1016/j.bbamcr.2017.11.009 Botelho, Hugo; Amaral, Margarida D.; Farinha, Carlos M.; Matos, Paulo; Botelho, Hugo M.. "Investigating Alternative Transport of Integral Plasma Membrane Proteins from the ER to the Golgi: Lessons from the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR).". Methods in molecular biology (Clifton, N.J.) (2016): 105-126. http://europepmc.org/abstract/med/27665554. Published • 10.1007/978-1-4939-3804-9_7 Igreja, Susana; Clarke, Luka A.; Botelho, Hugo M; Marques, Luís; Amaral, Margarida D.. "Correction of a Cystic Fibrosis Splicing Mutation by Antisense Oligonucleotides". Human Mutation 37 2 (2015): 209-215. http://dx.doi.org/10.1002/humu.22931. Published • 10.1002/humu.22931 Clarke, Luka A.; Botelho, Hugo M; Sousa, Lisete; Falcao, Andre O.; Amaral, Margarida D.. "Transcriptome meta-analysis reveals common differential and global gene expression profiles in cystic fibrosis and other respiratory disorders and identifies CFTR regulators". Genomics 106 5 (2015): 268-277. http://dx.doi.org/10.1016/j.ygeno.2015.07.005. Published • 10.1016/j.ygeno.2015.07.005 Botelho, Hugo M; Uliyakina, Inna; Awatade, Nikhil T.; Proença, Maria C.; Tischer, Christian; Sirianant, Lalida; Kunzelmann, Karl; Pepperkok, Rainer; Amaral, Margarida D.. "Protein Traffic Disorders: an Effective High-Throughput Fluorescence Microscopy Pipeline for Drug Discovery". Scientific Reports 5 1 (2015): http://dx.doi.org/10.1038/srep09038. Published • 10.1038/srep09038 Igreja, S.; Clarke, L. A.; Botelho, H. M.; Marques, L.; Amaral, M. D.. "Correction of a cystic fibrosis splicing mutation by antisense oligonucleotides". Human Mutation (2015): Carvalho, Sofia B.; Botelho, Hugo M; Leal, Sónia S.; Cardoso, Isabel; Fritz, Günter; Gomes, Cláudio M.. "Intrinsically Disordered and Aggregation Prone Regions Underlie ß-Aggregation in S100 Proteins". PLoS ONE 8 10 (2013): e76629. http://dx.doi.org/10.1371/journal.pone.0076629. Published • 10.1371/journal.pone.0076629 Botelho, Hugo M; Dahimène, S.; Uliyakina, I.; Neumann, B.; Tischer, C.; Pepperkok, R.; Amaral, M.D.. "WS20.6 High throughput screening as a source for novel cystic fibrosis therapeutic targets". Journal of Cystic Fibrosis 12 (2013): S42. http://dx.doi.org/10.1016/s1569-1993(13)60128-9. 10.1016/s1569-1993(13)60128-9 Dahimène, S.; Romão, A.; Botelho, Hugo M; Palma, M.; Correia, A.R.; Farinha, C.M.; King-Underwood, J.; et al. "WS21.9 Screening for novel F508del-CFTR correctors identified by pharmacophore modelling through automated microscopy and NBD1 folding assays". Journal of Cystic Fibrosis 12 (2013): S45. http://dx.doi.org/10.1016/s1569-1993(13)60137-x. Published • 10.1016/s1569-1993(13)60137-x Sá-Moura, Bebiana; Simões, Ana Marisa; Fraga, Joana; Fernandes, Humberto; Abreu, Isabel A.; Botelho, Hugo M; Gomes, Cláudio M.; et al. "Biochemical and biophysical characterization of recombinant yeast proteasome maturation factor Ump1". Computational and Structural Biotechnology Journal 7 8 (2013): e201304006. http://dx.doi.org/10.5936/csbj.201304006. Published • 10.5936/csbj.201304006 Veith, A.; Botelho, Hugo M; Kindinger, F.; Gomes, C.M.; Kletzin, A.. "The sulfur oxygenase reductase from the mesophilic bacterium Halothiobacillus neapolitanus is a highly active thermozyme". Journal of Bacteriology 194 3 (2012): 677-685. http://www.scopus.com/inward/record.url?eid=2-s2.0-84855915891&partnerID=MN8TOARS. Published • 10.1128/JB.06531-11 Leal, S.S.; Botelho, Hugo M; Gomes, C.M.. "Metal ions as modulators of protein conformation and misfolding in neurodegeneration". Coordination Chemistry Reviews 256 19-20 (2012): 2253-2270. http://www.scopus.com/inward/record.url?eid=2-s2.0-84864548981&partnerID=MN8TOARS. Published • 10.1016/j.ccr.2012.04.004 Botelho, Hugo M; Leal, S.S.; Cardoso, I.; Yanamandra, K.; Morozova-Roche, L.A.; Fritz, G.; Gomes, C.M.. "S100A6 amyloid fibril formation is calcium-modulated and enhances superoxide dismutase-1 (SOD1) aggregation". Journal of Biological Chemistry 287 50 (2012): 42233-42242. http://www.scopus.com/inward/record.url?eid=2-s2.0-84871281215&partnerID=MN8TOARS. Published • 10.1074/jbc.M112.396416 Botelho, Hugo M; Gomes, C.M.. "Structural reorganization renders enhanced metalloprotein stability". Chemical Communications 47 39 (2011): 11149-11151. http://www.scopus.com/inward/record.url?eid=2-s2.0-80053318341&partnerID=MN8TOARS. Published • 10.1039/c1cc13354c Fritz, G.; Botelho, Hugo M; Morozova-Roche, L.A.; Gomes, C.M.. "Natural and amyloid self-assembly of S100 proteins: Structural basis of functional diversity". FEBS Journal 277 22 (2010): 4578-4590. http://www.scopus.com/inward/record.url?eid=2-s2.0-78049277867&partnerID=MN8TOARS. Published • 10.1111/j.1742-4658.2010.07887.x Botelho, Hugo M; Leal, S.S.; Veith, A.; Prosinecki, V.; Bauer, C.; Fröhlich, R.; Kletzin, A.; Gomes, C.M.. "Role of a novel disulfide bridge within the all-beta fold of soluble Rieske proteins". Journal of Biological Inorganic Chemistry 15 2 (2010): 271-281. http://www.scopus.com/inward/record.url?eid=2-s2.0-77949270785&partnerID=MN8TOARS. Published • 10.1007/s00775-009-0596-3 Botelho, Hugo M; Koch, M.; Fritz, G.; Gomes, C.M.. "Metal ions modulate the folding and stability of the tumor suppressor protein S100A2". FEBS Journal 276 6 (2009): 1776-1786. http://www.scopus.com/inward/record.url?eid=2-s2.0-61349168402&partnerID=MN8TOARS. Published • 10.1111/j.1742-4658.2009.06912.x Prosinecki, V.; Botelho, Hugo M; Francese, S.; Mastrobuoni, G.; Moneti, G.; Urich, T.; Kletzin, A.; Gomes, C.M.. "A proteomic approach toward the selection of proteins with enhanced intrinsic conformational stability". Journal of Proteome Research 5 10 (2006): 2720-2726. http://www.scopus.com/inward/record.url?eid=2-s2.0-33750201140&partnerID=MN8TOARS. 10.1021/pr0602491

Intellectual property

Patent	Margarida D. Amaral; Botelho, Hugo M; Lopes-Pacheco, Miquéias. 2023. "Method of identifying agents for the treatment of cystic fibrosis caused by the mutation F508del". Granted/Issued
Provisional application for patent	2022. "Method of identifying agents for the treatment of cystic fibrosis caused by the mutation F508del". Protected

Activities

Oral presentation

	Presentation title	Event name Host (Event location)
2023/10/25	High content screening methods and algorithms for basic and applied research on Cystic Fibrosis	SPAOM2023 - Spanish & Portuguese Advanced Optical Microscopy Meeting PPBI & REMOA (Faro, Portugal)
2023/06/10	Global functional genomics reveals GRK5 as a therapeutic target for cystic fibrosis	46th ECFS Conference European Cystic Fibrosis Society (Vienna, Austria)
2023/05/12	High content screening from a core facility perspective: from experiments to discoveries	5th NEUBIAS Symposium Network of European BioImage Analysts (Porto, Portugal)
2022/04/01	New kinase therapeutic targets for Cystic Fibrosis from a global functional genomics screen	17th ECFS Basic Science Conference European Cystic Fibrosis Conference (Albufeira, Portugal)
2022	37 lectures and workshops on Cystic Fibrosis, Microscopy and Bioimage Analysis at national and international events.
2021/11/03	Functional genomics reveals global genes rescuing F508del-CFTR traffic and novel kinase therapeutic targets complementing current correctors	35th North American Cystic Fibrosis Conference Cystic Fibrosis Foundation (United States)
2021/02/04	High-throughput microscopy and screening	Optical microscopy at COLife and Friends: learning the basics COLife (Lisboa, Portugal)
2020/11/26	VALHealth – Valorisation of ALgae for Health: Bioactive compounds from marine bioresources by membrane technology	BioISI Research Day 2020 BioISI (Lisboa, Portugal)
2020/11/24	High content image analysis with CellProfiler	SPAOM2020: Spanish Portuguese Meeting for Advanced Optical Microscopy REMOA / PPBI (Valencia, Spain)
2020/09/24	Screening de novos e antigos fármacos: um modulador para a prevenção de metástases cerebrais do cancro da mama	2º Encontro Nacional de Jovens Investigadores em Oncologia Liga Portuguesa Contra o Cancro (Portugal)
2020/06/30	PPBI - Plataforma Portuguesa de BioImagem	6º Fórum de Gestão de Dados de Investigação FCT-FCCN – Fundação para a Ciência e Tecnologia (Portugal)
2019/11/13	Breast cancer cells extravasation into the brain: a dynamic trafficking across blood-brain barrier endothelium	5th Annual Meeting of the Mind-Brain College of the University of Lisbon University of Lisbon (Lisbon, Portugal)
2019/11/06	Analysis of time lapse microscopy data with CellProfiler et al.	3rd Spanish-Portuguese Meeting for Advanced Optical Microscopy – SPAOM2019 Red Española de Microscopía Óptica Avanzada & Portuguese Platform of BioImaging (Coimbra, Portugal)
2019/10/01	A systems view of Cystic Fibrosis: modeling the folding and maturation pathways of the CFTR protein	2nd CORBEL Service Operator and User Meeting CORBEL (Heidelberg, Germany)
2018/12/06	shinyHTM: an open-source web application for the analysis of high throughput microscopy data	EMBL Conference: From Images to Knowledge with ImageJ & Friends (I2K) European Molecular Biology Laboratory (Heidelberg, Germany)
2018/10/26	Innovating the search for novel Cystic Fibrosis therapies using high content microscopy screening	SPAOM2018: Spanish Portuguese Meeting for Advanced Optical Microscopy Red Española de Microscopía Óptica Avanzada & Portuguese Platform of BioImaging (Granada, Spain)
2018/01/22	A systems view of Cystic Fibrosis: modeling the folding and maturation pathways of the CFTR protein	CORBEL Work Package 4 Service Operator meeting CORBEL (Berlin, Germany)
2017/06/09	CFTR traffic – Functional genomics screens to identify novel regulators	40th European Cystic Fibrosis Conference European Cystic Fibrosis Society (Seville, Spain)
2017/04/01	A high-content siRNA screen at near genomic scale reveals novel F508del-CFTR therapeutic targets	14th ECFS Basic Science Conference European Cystic Fibrosis Society (Albufeira, Portugal)
2017/02/15	Modular high content image analysis workflows leveraging biology and biomedicine	NEUBIAS2020 BioImage Analysis Symposium Network of European BioImage Analysts - NEUBIAS (Lisbon, Portugal)
2016/10/13	Innovating the search for novel Cystic Fibrosis therapies using high content microscopy screening	BioISI Post Grad Seminar Biosystems & Integrative Sciences Institute (Lisbon, Portugal)
2015/11/13	Assessment of CFTR activity ex vivo as a basis for personalised medicine	5th Structure and Function of Ionic Channels and Transporters Workshop Faculty of Pharmacy of the University of Lisbon (Lisbon, Portugal)
2015/06/02	A high throughput fluorescence microscopy pipeline for drug discovery in Cystic Fibrosis	1st Course on High Content Screening and Image Analysis for Biosciences i3S - Instituto de Investigação e Inovação da Universidade do Porto (Porto, Portugal)
2015/05/10	S100 proteins as novel metal-ion regulated molecular chaperones in neurodegeneration	EMBO Conference: Molecular chaperones: From molecules to cells and misfolding diseases European Molecular Biology Organization (Heraklion, Greece)
2014/08/15	A high content screening system for the development of CFTR targeting therapies in the genetic disease Cystic Fibrosis	International ITU Molecular Biology and Genetics Students Congress ‘14 Istanbul Technical University (Istanbul, Turkey)
2014/03/26	A high-content siRNA screens identify novel CFTR traffic regulators in human airway epithelial cells	11th ECFS Basic Science Conference European Cystic Fibrosis Society (St. Julians, Malta)
2013/10/17	High throughput screen reveals genes regulating wt-CFTR and rescuing F508del-CFTR traffic	27th Annual North American Cystic Fibrosis Conference Cystic Fibrosis Foundation (Salt Lake City, United States)
2013/06/14	High throughput screening as a source for novel Cystic Fibrosis therapeutic targets	36th European Cystic Fibrosis Society Conference European Cystic Fibrosis Society (Lisbon, Portugal)
2013/05/17	Cross talk between S100 proteins and amyloid-forming proteins in neurodegeneration	EMBO Conference: The biology of molecular chaperones: From molecules, organelles and cells to misfolding diseases European Molecular Biology Organization (Santa Margherita di Pula, Italy)
2013/05/14	Screening for novel F508del-CFTR correctors identified by pharmacophore modelling through automated microscopy and NBD1 folding assays	36th European Cystic Fibrosis Society Conference European Cystic Fibrosis Society (Lisbon, Portugal)
2013/03/07	High-throughput identification of CFTR traffic regulators	BioFIG PhD Student/Post-Doc Seminar Center for Biodiversity, Functional & Integrative Genomics (Lisbon, Portugal)
2011/01/19	Aß fibrils and metal ions modulate S100 amyloidogenesis: novel players in neurodegeneration?	Lisbon Area Neuroscience Meeting Instituto de Medicina Molecular (Lisbon, Portugal)
2010/07/08	Metal ions and Aß fibrils modulate S100 amyloidogenesis	IV Spanish Portuguese Biophysical Congress Sociedad de Biofísica de España, Portuguese Biophysical Society (Zaragoza, Spain)
2009/11/04	Metal ions as modulators of folding and amyloidogenic pathways in the S100 protein family	ITQB PhD Seminar Instituto de Tecnologia Química e Biológica (Oeiras, Portugal)
2007/02/02	Modulation of the stability of S100A2 variants by metal ions	Workshop Proteins in Health and Disease: from structure to function Faculty of Pharmacy, University of Lisbon (Lisbon, Portugal)

Supervision

	Thesis Title Role	Degree Subject (Type) Institution / Organization
2023/09/04 - 2024/09/01	Characterizing the regulation of the CFTR protein by the kinase GRK5 in cystic fibrosis models Supervisor	Bioquímica (Master) Universidade de Lisboa Faculdade de Ciências, Portugal
2023/04/01 - 2023/10/01	Regulation of the intracellular traffic of p.Phe508del-CFTR by the kinase GRK5 in Cystic Fibrosis models Supervisor of Daniela Neugebauer	Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2022/09 - 2023/07	Cristais de spin lábil termo e foto-sensíveis para músculos artificiais Co-supervisor of André Fernandes	Química (Degree) Universidade de Lisboa Faculdade de Ciências, Portugal
2021/09/20 - 2022/07/15	Trainee bioimage analyst at BioISI’s High-Throughput Screening Facility Supervisor of Pau Rubio Costa	Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2020/09/29 - 2022/01/31	Application of new bioactive compounds extracted from algae: health benefits and effects on Cystic Fibrosis Co-supervisor of Ana Patrícia Rodrigues Duarte	Engenharia Química e Biológica (Master) Instituto Politécnico de Lisboa Instituto Superior de Engenharia de Lisboa, Portugal
2021/07/17 - 2021/12/31	Laboratory rotation, part of PhD course. Time-lapse confocal imaging of mixed 3D cell cultures. Optimization of a cellular extravasation assay Supervisor of Ana Rita da Costa Garcia	Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2018/03/05 - 2021/03/31	Bioimage analyst at BioISI High-Throughput Screening Facility Supervisor of Aires Miguel Gomes Delgado de Almeida Duarte	Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2020/12/03 - 2020/12/03	Laboratory rotation, part of PhD course. Confocal imaging of mixed 3D cell cultures; tools for 3D data visualization and cell tracking Supervisor of Joana Godinho Pereira	Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2019/09/06 - 2020/09/17	Effect of Novel CFTR Interactors on Traffic of F508del Mutant: Implications for Cystic Fibrosis Co-supervisor of Joana Cartaxo Serralha	Biomedical Science (Degree) King's College London Faculty of Life Sciences and Medicine, United Kingdom Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2018/02/01 - 2018/12/31	Deconvolution of dual CFTR/ANO1 Modulators from Portuguese natural products - A new class of drugs for CF therapy Supervisor of Joana Godinho-Pereira	Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2016/05/01 - 2017/12/31	The identification of new natural compounds of high therapeutic potential for Cystic Fibrosis by high-throughput microscopy screens Supervisor of Catarina Duarte Galhardo Baptista	Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2014 - 2015	Effect of TGFß signaling on CFTR protein: consequences for epithelial differentiation in CF Co-supervisor of Margarida Cuco Quaresma	Bioquímica (Master) Universidade de Lisboa Faculdade de Ciências, Portugal
2010 - 2011	Molecular and mechanistic aspects of the effect of metal ions in the conformation and amyloidogenesis of the S100B protein Co-supervisor of Sofia Carvalho	Bioquímica (Master) Universidade de Lisboa Faculdade de Ciências, Portugal

Event organisation

	Event name Type of event (Role)	Institution / Organization
2024/04/15 - 2024/04/19	Advanced Course on the Principles of Light Microscopy (2024/04/15 - 2024/04/19) Workshop (Member of the Organising Committee)	Universidade de Lisboa Faculdade de Ciências, Portugal
2023/12/11 - 2023/12/13	Introduction to Image Analysis (2023/12/11 - 2023/12/13) Workshop (Member of the Organising Committee)	Universidade de Lisboa Faculdade de Ciências, Portugal
2023/03 - 2023/10/27	SPAOM2023 – Spanish-Portuguese Advanced Optical Microscopy 2023 (2023/10/25 - 2023/10/27) Congress (Member of the Scientific Committee)
2023/08/28 - 2023/08/30	Training on confocal microscopy and core facility management (2023/08/28 - 2023/08/30) Workshop (President of the Organising Committee)	Universidade de Lisboa Faculdade de Ciências, Portugal
2023/03/20 - 2023/03/22	Macro Scripting in ImageJ - COLife & FCUL (2023/03/20 - 2023/03/22)
2022/12/13 - 2022/12/16	Basics in Light Microscopy (2022/12/13 - 2022/12/16) Workshop (President of the Organising Committee)	Universidade de Lisboa Faculdade de Ciências, Portugal
2022/09/21 - 2022/09/23	Introduction to Image Analysis - COLife & FCUL Course (2022/09/21 - 2022/09/23) Workshop (Member of the Organising Committee)	Universidade de Lisboa Faculdade de Ciências, Portugal
2022/07/15 - 2022/07/15	Chem&BioChem – Postgraduate Students Meeting, FCUL (2022/07/15 - 2022/07/15) Meeting (Member of the Scientific Committee)	Universidade de Lisboa Faculdade de Ciências, Portugal
2022/06/22 - 2022/06/22	Hands-on CellProfiler training for Lisbon Area Bioimaging Group members (2022/06/22 - 2022/06/22) Workshop (President of the Organising Committee)	Universidade de Lisboa Faculdade de Ciências, Portugal
2022/01/18 - 2022/01/20	Macro Scripting in ImageJ - COLife & FCUL (2022/01/18 - 2022/01/20) Workshop (Co-organisor)
2021/11/23 - 2021/11/25	SPAOM2021 – Spanish-Portuguese Advanced Optical Microscopy 2021 (2021/11/23 - 2021/11/25) Congress (Member of the Scientific Committee)	Portuguese Platform of BioImaging, Portugal
2021/10/25 - 2021/10/28	Basics in Light Microscopy (2021/10/25 - 2021/10/28) Workshop (President of the Organising Committee)	Universidade de Lisboa Faculdade de Ciências, Portugal
2021/09/21 - 2021/09/23	Introduction to Image Analysis COLife & FCUL (2021/09/21 - 2021/09/23) Workshop (Co-organisor)
2021/02/15 - 2021/02/17	Eutopia 3 - Third Meeting of the European Topology Interdisciplinary Initiative (2021/02/15 - 2021/02/17) Meeting (Member of the Organising Committee)	Universidade de Lisboa Faculdade de Ciências, Portugal
2019/06/11 - 2019/06/12	Workshop on Integrative Approaches to Protein Folding & Aggregation (2019/06/11 - 2019/06/12) Workshop (Member of the Organising Committee)	Universidade de Lisboa Faculdade de Ciências, Portugal
2018/07/09 - 2018/07/13	Hands-on Workshop on High-Throughput Microscopy (2018/07/09 - 2018/07/13) Workshop (Co-organisor)	Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2017/07/17 - 2017/07/21	Hands-on Workshop on High-Throughput Microscopy (2017/07/17 - 2017/07/21) Workshop (Co-organisor)	Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2016/07/11 - 2016/07/15	Hands-on Workshop on High-Throughput Microscopy (2016/07/11 - 2016/07/15) Workshop (Co-organisor)	Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2015/03/18 - 2015/03/20	Hands-on Workshop on High-Throughput Microscopy (2015/03/18 - 2015/03/20) Workshop (Co-organisor)	Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal

Event participation

	Activity description Type of event	Event name Institution / Organization
2022/03/30 - 2022/04/02	17th ECFS Basic Science Conference Congress	17th European Cystic Fibrosis Society Basic Science Conference
2021/11/30 - 2021/11/30	5.ª Conferência Anual da redeSAÚDE Conference	5.ª Conferência Anual da redeSAÚDE Universidade de Lisboa, Portugal
2021/11/23 - 2021/11/25	SPAOM2021 Congress	Spanish & Portuguese Advanced Optical Microscopy Meeting
2021/11/02 - 2021/11/05	35th NACFC Congress	35th North American Cystic Fibrosis Conference
2021/02/02 - 2021/02/04	Optical microscopy at COLife & Friends: learning the basics Workshop	Optical microscopy at COLife and Friends: learning the basics
2020/11/24 - 2020/11/27	SPAOM2020 Congress	Spanish & Portuguese Advanced Optical Microscopy Meeting
2020/09/24 - 2020/09/25	43rd ECFS Conference Congress	43rd European Cystic Fibrosis Conference
2020/06/30 - 2020/07/01	6º Fórum GDI Conference	6º Fórum de Gestão de Dados de Investigação Portuguese Platform of BioImaging, Portugal
2019/11/06 - 2019/11/08	SPAOM2019 Meeting	3rd Spanish-Portuguese Meeting for Advanced Optical Microscopy – SPAOM2019 Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2019/10/01 - 2019/10/02	2nd CORBEL Service Operator and User Meeting Meeting	2nd CORBEL Service Operator and User Meeting Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2019/06/19 - 2019/06/19	Preclinical Imaging Lifesciences Workshop	Preclinical Imaging Lifesciences Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2018/12/05 - 2018/12/08	From images to knowledge with ImageJ & friends (I2K) Conference	EMBL Conference: From images to knowledge with ImageJ & friends (I2K) and I2K Developers Satellite Meeting European Molecular Biology Laboratory, Germany
2018/10/23 - 2018/10/26	SPAOM2018 Meeting	2nd Spanish-Portuguese Meeting for Advanced Optical Microscopy – SPAOM2018 Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2018/02/22 - 2018/02/23	CORBEL WP4 Service Operator Meeting Meeting	CORBEL WP4 Service Operator Meeting Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2017/09/17 - 2017/09/21	EMBO Workshop Workshop	EMBO Workshop – Proteostasis European Molecular Biology Organization, Germany
2017/06/07 - 2017/06/10	40th European Cystic Fibrosis Conference Conference	40th European Cystic Fibrosis Conference European Cystic Fibrosis Society, Denmark
2017/06/06 - 2017/06/09	3rd Course on High Content Screening and Image Analysis for BioSciences Other	3rd Course on High Content Screening and Image Analysis for BioSciences Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2017/05/08 - 2017/05/08	3rd Annual Conference Rede Mar Conference	3rd Annual Conference Rede Mar Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2017/03/29 - 2017/04/01	14th ECFS Basic Science Conference Conference	14th ECFS Basic Science Conference European Cystic Fibrosis Society, Denmark
2017/02/15 - 2017/02/17	NEUBIAS2020 BioImage Analysis Symposium Symposium	NEUBIAS2020 BioImage Analysis Symposium European Cooperation in Science and Technology, Belgium
2016/07/04 - 2016/07/06	Ciência 2016 Meeting	Ciência 2016 - Science and Technology in Portugal Meeting Fundação para a Ciência e a Tecnologia, Portugal
2016/06/14 - 2016/06/17	2nd Course on High Content Screening and Image Analysis for Biosciences Other	2nd Course on High Content Screening and Image Analysis for Biosciences Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2015/06/01 - 2015/06/04	1st Course on High Content Screening and Image Analysis for Biosciences Other	1st Course on High Content Screening and Image Analysis for Biosciences Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2015/03/25 - 2015/03/28	12th ECFS Basic Science Conference Conference	12th ECFS Basic Science Conference European Cystic Fibrosis Society, Denmark
2014/08/15 - 2014/08/18	ITU Molecular Biology and Genetics Students Congress Congress	International ITU Molecular Biology and Genetics Students Congress ’14 Istanbul Teknik Üniversitesi, Turkey European Molecular Biology Organization, Germany
2014/05/10 - 2014/05/15	ESF/EMBO Cell Polarity and Membrane Trafficking Conference	ESF/EMBO Cell Polarity and Membrane Trafficking European Molecular Biology Organization, Germany European Science Foundation, France
2013/10/17 - 2013/10/19	27th Annual NACFC Conference	27th Annual North American Cystic Fibrosis Conference Cystic Fibrosis Foundation, United States European Molecular Biology Organization, Germany
2013/09/21 - 2013/09/24	The 5th EMBO Meeting Meeting	The 5th EMBO Meeting European Molecular Biology Organization, Germany
2013/07/18 - 2013/07/18	Molecular Biology in Portugal and EMBL Meeting	Molecular Biology in Portugal and EMBL Universidade de Lisboa Faculdade de Ciências, Portugal
2013/06/12 - 2013/06/15	36th ECFS Conference Conference	36th European Cystic Fibrosis Society Conference European Cystic Fibrosis Society, Denmark Deutschen Gesellschaft für Neuropathologie und Neuroanatomie, Germany
2011/10/26 - 2011/10/28	2nd ITQB PhD Students’ Meeting Meeting	2nd ITQB PhD Students’ Meeting Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal Deutschen Gesellschaft für Neuropathologie und Neuroanatomie, Germany
2011/09/21 - 2011/09/24	56th Annual Meeting DGNN Meeting	56th Annual Meeting, German Society for Neuropathology and Neuroanatomy Deutschen Gesellschaft für Neuropathologie und Neuroanatomie, Germany
2011/05/05 - 2011/05/07	Interbio Symposium Symposium	Interbio Symposium 'Frontiers in Protein Research' Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2010/10/21 - 2010/10/22	1st ITQB PhD Students' Meeting Meeting	1st ITQB PhD Students' Meeting Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2010/07/07 - 2010/07/10	IV Spanish-Portuguese Biophysical Congress Congress	IV Spanish-Portuguese Biophysical Congress Sociedade Portuguesa de Biofísica, Portugal Sociedad de Biofísica de España, Spain
2010/01/10 - 2010/01/15	Gordon Research Conference Conference	Gordon Research Conference “Protein Folding Dynamics”
2010/01/09 - 2010/01/10	Gordon Research Seminar Seminar	Gordon Research Seminar “Protein Folding Dynamics”
2009/10/30 - 2009/11/01	8th Short Course of the Portuguese Biophysical Society Other	8th Short Course of the Portuguese Biophysical Society “Systems Biology” Sociedade Portuguesa de Biofísica, Portugal
2009/06/14 - 2009/06/18	VIII European Symposium of The Protein Society Symposium	VIII European Symposium of The Protein Society Protein Society, United States
2009/03/19 - 2009/03/19	Protein Folding Diseases: From Metabolic Disorders to Neurodegeneration Workshop	International Workshop “Protein Folding Diseases: From Metabolic Disorders to Neurodegeneration” Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2008/10/02 - 2008/10/03	Workshop Physics of Biological Systems Workshop	Workshop Physics of Biological Systems: From Genes to Societies Universidade de Lisboa Centro de Física Teórica e Computacional, Portugal
2008/09/02 - 2008/09/06	EUROBIC9 Conference	The 9th European Biological Inorganic Chemistry Conference, EUROBIC9 Society of Biological Inorganic Chemistry, United States
2008/07/27 - 2008/08/01	FASEB Summer Research Conference Conference	FASEB Summer Research Conference – Protein Folding in the Cell Federation of American Societies for Experimental Biology, United States
2008/03/07 - 2008/03/07	1st Symposium iMed.UL Symposium	1st Symposium iMed.UL Universidade de Lisboa Faculdade de Farmácia, Portugal
2007/07/15 - 2007/07/20	ICBIC XIII Conference	13th International conference on Biological Inorganic Chemistry, ICBIC XIII Society of Biological Inorganic Chemistry, United States
2007/05/12 - 2007/05/16	VII European Symposium of the Protein Society Symposium	VII European Symposium of the Protein Society – From Proteins to Proteome Protein Society, United States
2007/02/02 - 2007/02/03	Workshop Proteins in Health and Disease Workshop	Workshop Proteins in Health and Disease: from Structure to Function Universidade de Lisboa Faculdade de Farmácia, Portugal
2006/11/08 - 2006/11/08	A one day workshop on protein science Workshop	A one day workshop on protein science Universidade de Lisboa Centro de Física Teórica e Computacional, Portugal
2006/07/01 - 2006/07/06	EUROBIC8 Conference	EUROBIC8 – 8th European Biological Inorganic Chemistry Conference and Young Researchers Forum Society of Biological Inorganic Chemistry, United States

Jury of academic degree

	Topic Role	Candidate name (Type of degree) Institution / Organization
2022/03/21	Amyloids in Nature: structural analysis of sea urchin inspired adhesive protein (Thesis) Main arguer	André Gonzaga Ribeiro de Castro Fernandes (Master) Universidade de Lisboa Faculdade de Ciências, Portugal
2022/03/21	Amyloids in Nature: structural analysis of sea urchin inspired adhesive protein (Thesis) Main arguer	André Gonzaga Ribeiro de Castro Fernandes (Master) Universidade de Lisboa Faculdade de Ciências, Portugal

Committee member

	Activity description Role	Institution / Organization
2023/12 - Current	Chair of Image Analysis Working Group Coordinator	Portuguese Platform of BioImaging, Portugal
2022 - Current	Member of PhD thesis advisory committee - Maria Joana Valente Ribeiro PhD in Biochemistry Member	Universidade de Lisboa Faculdade de Ciências, Portugal
2019/07 - Current	High-Throughput Screening Facility, Manager Coordinator	Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2019/07 - Current	FCUL Microscopy Facility, Co-Responsible Coordinator	Universidade de Lisboa Faculdade de Ciências, Portugal

Course / Discipline taught

	Academic session	Degree Subject (Type)	Institution / Organization
2024 - Current	Experimental Biochemistry IV	Bioquímica (Licenciatura)	Universidade de Lisboa Faculdade de Ciências, Portugal
2022/02 - 2024/07	Fundamentos de Química e Bioquímica [Fundamentals in Chemistry and Biochemistry]	Engenharia Biomédica e Biofísica (Licenciatura)	Universidade de Lisboa Faculdade de Ciências, Portugal
2023/02 - 2023/07	Introdução à Biologia Molecular [Introduction to Molecular Biology]	Bioquímica (Licenciatura)	Universidade de Lisboa Faculdade de Ciências, Portugal
2023/02 - 2023/07	Projeto [Project]	Química (Licenciatura)	Universidade de Lisboa Faculdade de Ciências, Portugal
2020/02 - 2020/06	Biochemistry	Biologia (Licenciatura)	Universidade de Lisboa Faculdade de Ciências, Portugal

Evaluation committee

	Activity description Role	Institution / Organization	Funding entity
2021/07/29 - Current	Maria Joana Valente Ribeiro Main opponent: Seminário de Investigação I (1st year PhD discussion) Evaluator	Universidade de Lisboa Faculdade de Ciências, Portugal
2021 - Current	Effective jury member for hiring an MSc student for CFF grant “Rep2CFTR: Repurposing FDA-Approved Drugs for Class II Rare CFTR Mutants”. PI: Miquéias Lopes-Pacheco Evaluator	Universidade de Lisboa Faculdade de Ciências, Portugal
2020/02/21 - Current	Jury member for hiring a PhD researcher for FCT project “ProDysMito” Reference PTDC/BIA-BQM/29963/2017 PI: Bárbara J Henriques Evaluator	Fundação da Faculdade de Ciências da Universidade de Lisboa, Portugal	Fundação para a Ciência e a Tecnologia

Journal scientific committee

	Journal title (ISSN)	Publisher
2023/05/02 - Current	Healthcare (2227-9032)	MDPI
2023/03 - Current	Cells (2073-4409)	MDPI
2023 - Current	Journal of Microscopy (1365-2818)	Wiley
2023 - Current	International Journal of Molecular Sciences (1422-0067)	MDPI
2022/11 - Current	Medicina (1648-9144)	MDPI
2021 - Current	Molecular Genetics & Genomic Medicine (2324-9269)	Wiley Periodicals, LLC
2021 - Current	Journal of Visualized Experiments (1940-087X)
2018/11 - Current	SLAS DISCOVERY Advancing the Science of Drug Discovery (2472-5560)	SAGE Publications
2016/11 - Current	Scientific Reports (2045-2322)	Springer Science and Business Media LLC
2014/11 - Current	PLoS ONE (1932-6203)	Public Library of Science
2013/08 - Current	Biochimie (0300-9084)	Elsevier
2013/02 - Current	Metallomics (1756-591X)	The Royal Society of Chemistry
2012/12 - Current	FEBS Letters (1873-3468)	Wiley (John Wiley & Sons)
2012/12 - Current	Journal of Dermatological Science (0923-1811)	Elsevier
2011 - Current	Physical Chemistry Chemical Physics (1463-9076)	Royal Society of Chemistry
2011 - Current	Journal of Biotechnology (0168-1656)	Elsevier

Mentoring / Tutoring

	Topic	Student name
2011/01/01 - 2011/01/31	Conformation and Stability of Phenylalanine Hydroxylase	João Leandro
2010/05/01 - 2010/12/31	Molecular basis of the stabilization of the Acidianus ambivalens Rieske Ferredoxin by using rationally designed mutants	Francesco Barbero
2010/03 - 2010/03	Elucidation of the molecular basis of the stabilization of the Acidianus ambivalens Rieske Ferredoxin by using rationally designed mutants	Dennis Petrasch
2009/10/01 - 2009/10/31	Assembly of wild type and mutant Acidianus ambivalens Sulfur Oxygenase Reductase	Andreas Veith
2007/01/01 - 2007/03/31	Conformation and stability of Phenylalanine Hydroxylase	Cátia Nascimento

Distinctions

Award

2019	Travel grant. NEUBIAS Training School #13. BioImage Analysis for Facility Staff
2018	ITC Conference Grant - EMBL Conference "From Images to Knowledge with ImageJ & Friends" European Cooperation in Science and Technology, Belgium
2015	Meeting fellowship - 12th ECFS Basic Science Conference European Cystic Fibrosis Society, Denmark
2013	Best Poster Award at the meeting “Molecular Biology in Portugal and EMBL"
2011	FEBS Short Term Grant Federation of European Biochemical Societies, United Kingdom
2010	Subsidy for participating in International Congresses - IV Spanish-Portuguese Biophysical Congress) Fundação Calouste Gulbenkian, Portugal
2010	Travel Grant - IV Spanish-Portuguese Biophysical Congress
2010	Travel Grant - IV Spanish-Portuguese Biophysical Congress
2007	Banco Espírito Santo Award – Best Classified Graduates 2005/2006 – Biochemistry Course, Faculty of Sciences, University of Lisbon Banco Espírito Santo e Comercial de Lisboa, Portugal
2007	Student Travel Grant - 13th International Conference on Biological Inorganic Chemistry Society of Biological Inorganic Chemistry, United States
2004	Fundação da Faculdade de Ciências da Universidade de Lisboa Award – Best Classified 1st year Graduation Students in 2002/2003 Fundação da Faculdade de Ciências da Universidade de Lisboa, Portugal

Other distinction

2014	Post-Doc Fellowship (SFRH/BD/31126/2006) Fundação para a Ciência e a Tecnologia, Portugal
2012	Post-Doc Fellowship (Reference #7207534) Cystic Fibrosis Foundation, United States
2011	Post-Doc Fellowship (Research Project PTDC/QUI/70101/2006) Fundação para a Ciência e a Tecnologia, Portugal
2006	PhD Fellowship (SFRH/BD/31126/2006) Fundação para a Ciência e a Tecnologia, Portugal