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Current research interests include the development of new separation processes with high performance and efficiency for purification biopharmaceuticals, with special emphasis on the purification of antibodies and the development of devices- "Lab-on-a-Chip" - for protein purification / separation of cells in the microscale.
Identification

Personal identification

Full name
Raquel Aires Barros

Citation names

  • Aires-Barros, M.R.

Author identifiers

Ciência ID
E818-26D2-5BEC
ORCID iD
0000-0002-1813-4280

Knowledge fields

  • Engineering and Technology - Industrial Biotechnology - Bioprocessing Technologies
Education
Degree Classification
1990/12/19
Concluded
 Ph.D. (1990) in Chemical Engineering from Instituto Superior Técnico (IST) (Título de Agregado)
Major in Biotechnology/Biochemical Engineering
Universidade de Lisboa Instituto Superior Técnico, Portugal
Affiliation

Teaching in Higher Education

Category
Host institution 		Employer
2011/12/17 - Current Full Professor (University Teacher) Universidade de Lisboa Instituto Superior Técnico, Portugal
Universidade de Lisboa Instituto Superior Técnico, Portugal
Projects

Grant

Designation Funders
2024 - Current Digitalisation of Biomanufacturing of Plasmids for the Development of Advanced Therapy Modalities
HORIZON-AG-LS
 Horizon 2020
2019/01 - Current Continuous Downstream Processing of Bioproducts Project acronym: CODOBIO
H2020-MSCA-ITN-2018
 EU Framework Programme for Research and Innovation Marie Sklodowska-Curie Actions

Contract

Designation Funders
2022/01/01 - 2024/12/31 Clean Technologies for Pure Water: Eliminating micropollutants from drinking water
PTDC/EAM-AMB/2023/2021
Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal

Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal

Universidade de Lisboa Centro de Química Estrutural, Portugal

Universidade Nova de Lisboa Associação para a Inovação e Desenvolvimento da FCT, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2018/05/01 - 2020/07/31 Phage4BacID
LISBOA-01-0247-FEDER-03303
Researcher
Associação para o Desenvolvimento do Instituto Superior Técnico, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2016/04/01 - 2019/06/30 PureMAb - Cromatografia multi-modal como uma alternativa à purificação de anticorpos monoclonais
PTDC/QEQ-PRS/0286/2014
Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal

Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2016/01/01 - 2019/03/31 Estratégias ópticas integradas para biosensores em sistemas "Lab on Chip" avançadas
PTDC/BBB-NAN/5927/2014
INESC Microsistemas e Nanotecnologias, Portugal

Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2012/08/01 - 2017/01/31 Gaining Productivity, Cost Efficiency and Sustainability in the Downstreaming Processing of Bio Products by novel Integration and Intensification strategies
info:eu-repo/grantAgreement/EC/FP7/312004/EU
Principal investigator
European Commission
Concluded
2012/01/01 - 2014/12/31 DSPMab - Intensificação do processo de purificação de anticorpos monoclonais
PTDC/EQU-EPR/117427/2010
Instituto de Biotecnologia e Bioengenharia, Portugal

Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2011/01/01 - 2013/12/31 Interacções Proteína-Meio Aquoso
PTDC/EQU-EQU/112812/2009
Universidade do Porto Laboratório de Processos de Separação e Reacção Laboratório de Catálise e Materiais, Portugal

Universidade do Porto Faculdade de Engenharia, Portugal

Universidade de Lisboa Instituto Superior Técnico, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2010/01/01 - 2013/12/31 Estratégias inovadoras para a purificação de biofarmacêuticos: Nanopartículas Magnéticas aplicadas a processos de Separação Magnética e de Sistemas de Duas Fases Aquosas
PTDC/EBB-BIO/098961/2008
Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal

Universidade de Lisboa Instituto Superior Técnico, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2010/01/01 - 2012/12/31 STEMSELECT - Novo processo de isolamento e expansão de células estaminais hematopoiéticas humanas
PTDC/EQU-EPR/104663/2008
Universidade de Lisboa Instituto Superior Técnico, Portugal

Instituto de Biotecnologia e Bioengenharia, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2007/10/01 - 2010/12/31 Caracterização Biológica e Molecular de Vírus da Gripe Aviária e Desenvolvimento de Testes DIVA
PTDC/CVT/67189/2006
Laboratório Nacional de Investigação Veterinária, Portugal

Instituto Nacional de Investigação Agrária e Veterinária, Portugal

Universidade de Lisboa Instituto Superior Técnico, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2007/10/01 - 2010/12/31 Síntese, optimização e intensificação de processos de biosseparação para produção de vacinas de DNA
PTDC/EQU-EQU/65492/2006
Universidade de Lisboa Instituto Superior Técnico, Portugal

Universidade de Lisboa Instituto Superior Técnico, Portugal

Universidade da Beira Interior, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2005/08/22 - 2010/07/31 Advanced Interactive Materials by Design"(AIMS)
NMP3-CT2004-500160
Principal investigator
Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
 European Commission
Concluded
2005/06/01 - 2008/06/30 Nanoparticulas para terapia génica e molecular
POCI/BIO/62476/2004
Universidade do Algarve, Portugal

Universidade do Algarve Faculdade de Ciências e Tecnologia, Portugal

Universidade de Lisboa Instituto Superior Técnico, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2005/06/01 - 2007/12/31 Racionalização da enantioselectividade da cutinase em meios não aquosos
POCI/BIO/57193/2004
Portuguesa Centro de Biotecnologia e Química Fina, Portugal

Universidade de Lisboa Instituto Superior Técnico, Portugal

Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
Outputs

Publications

Book
  1. Aires-Barros, M.R.; Azevedo, A.M.. Fundamentals of Biological Separation Processes. 2017.
  2. Ventura, S.P.M.; Nobre, B.P.; Ertekin, F.; Hayes, M.; Garciá-Vaquero, M.; Vieira, F.; Koc, M.; et al. Extraction of value-added compounds from microalgae. 2017.
    10.1016/B978-0-08-101023-5.00019-4
  3. Ahmed Samatou, J.; Engbert Wentink, A.; Alexandra J.Rosa, P.; Margarida Azevedo, A.; Raquel Aires-Barros, M.; Backer, W.; Gorak, A.. Modeling of counter current monoclonal antibody extraction using aqueous two-phase systems. 2007.
    10.1016/S1570-7946(07)80180-5
  4. Melo, E.P.; Aires-Barros, M.R.; Cabral, J.M.S.. Reverse micelles and protein biotechnology. 2001.
    10.1016/S1387-2656(01)07034-X
  5. Fontes, N.; Almeida, M.C.; Garcia, S.; Peres, C.; Grave, J.; Aires-Barros, M.R.; Soares, C.M.; et al. Cutinase activity and enantioselectivity in supercritical fluids. 1998.
    10.1016/S0921-0423(98)80072-5
Book chapter
  1. Capela, E.V.; Aires-Barros, M.R.; Mara G. Freire; Azevedo, A.M.. "Monoclonal Antibodies ¿ Addressing the Challenges on the Manufacturing Processing of an Advanced Class of Therapeutic Agents". In Frontiers in Clinical Drug Research-Anti-Infectives. Bentham Science, 2017.
  2. Sebastião, M.J.; Cabral, J.M.S.; Aires-Barros, M.R.. "Stability of a Fusarium solani pisi recombinant cutinase in reversed micelles". 473-480. 1993.
    10.1016/B978-0-444-89372-7.50062-2
  3. Sebastião, M.J.; Cabral, J.M.S.; Aires-Barros, M.R.. "Synthesis of fatty acid esters by a recombinant cutinase in reversed micelles". 719-724. 1992.
    10.1016/B978-0-444-89046-7.50103-X
Conference paper
  1. Soares, R.R.G.; Santos, D.R.; Pinto, I.F.; Azevedo, A.M.; Aires-Barros, M.R.; Chu, V.; Conde, J.P.. "Multiplexed microfluidic platform coupled with photodetector array for point-of-need and sub-minute detection of food contaminants". 2018.
    10.1109/MEMSYS.2018.8346467
  2. Pinto, I.F.; Santos, D.R.; Petrucci, G.; Soares, R.R.G.; Aires-Barros, M.R.; Chu, V.; Conde, J.P.; Azevedo, A.M.. "Development of a microfluidic device with integrated valves and photodiodes for rapid optimization of chromatographic separations of monoclonal antibodies". 2016.
  3. Soares, R.R.G.; Pinto, I.F.; Santos, D.R.; Azevedo, A.M.; Chu, V.; Aires-Barros, M.R.; Conde, J.P.. "A novel point-of-use, ultrafast and sensitive fluorescence immunoassay for the simultaneous detection of multiple mycotoxins using nanoporous agarose beads confined in microchannels". 2016.
  4. Santos, D.R.; Soares, R.R.G.; Chu, V.; Aires-Barros, M.R.; Conde, J.P.. "A Multiplexed Integrated a-Si:H Photosensor for Simultaneous Detection of Mycotoxins for Point-of-use Food Safety Applications". 2016.
    10.1016/j.proeng.2016.11.403
  5. Pinto, I.F.; Santos, D.R.; Soares, R.R.G.; Aires-Barros, M.R.; Chu, V.; Azevedo, A.M.; Conde, J.P.. "Integration of Photosensors in a Nano-liter Scale Chromatography Column for the Online Monitoring of Adsorption/Desorption Kinetics of a Fluorophore-labeled Monoclonal Antibody". 2016.
    10.1016/j.proeng.2016.11.405
  6. Soares, R.R.G.; Santos, D.R.; Pinto, I.F.; Azevedo, A.M.; Chu, V.; Aires-Barros, M.R.; Conde, J.P.. "Point-of-use Ultrafast Single-step Detection of Food Contaminants: A Novel Microfluidic Fluorescence-based Immunoassay with Integrated Photodetection". 2016.
    10.1016/j.proeng.2016.11.208
  7. Jacinto, M.J.; Azevedo, A.M.; Aires-Barros, M.R.; Archinti, M.; Marzabal, P.. "Extraction of Zera® fusion proteins in aqueous two-phase systems". 2015.
    10.1109/ENBENG.2015.7088835
  8. Soares, R.; Silva, D.F.C.; Azevedo, A.M.; Fernandes, P.; Chu, V.; Conde, J.P.; Aires-Barros, M.R.. "ATPS from natural products to large biomolecules". 2013.
  9. Azevedo, A.M.; Aires-Barros, M.R.. "New platforms for the downstream processing of biopharmaceuticals". 2011.
    10.1109/ENBENG.2011.6026043
  10. Azevedo, A.M.; Aires-Barros, M.R.. "Novel strategies for the purification of monoclonal antibodies". 2011.
    10.1109/ENBENG.2011.6026067
Conference poster
  1. P C de Barros, Dragana; Campos, Sara R. R.; Azevedo, Ana; Baptista, A.M.; Aires-Barros, M.R.. "Prediction of protein partition coefficients in aqueous two phase system". Paper presented in 34th International Symposium on the Separation of Proteins, Peptides and Polynucleotides, Würzburg , Germany, 2014.
  2. P C de Barros, Dragana; Campos, Sara R. R.; Madeira, Pedro; Azevedo, Ana; Baptista, A.M.; Aires-Barros, M.R.. "Study of a protein partitioning in aqueous two-phase system using a solvent continuum model". Paper presented in 9th International Conference on Protein Stabilisation,Lisbon, Portugal., 2012.
Journal article
  1. Alois Jungbauer; Peter Satzer; Astrid Duerauer; Ana Azevedo; Raquel Aires-Barros; Bernt Nilsson; Suzy Farid; et al. "Continuous downstream processing". Separation and Purification Technology (2024): http://dx.doi.org/10.1016/j.seppur.2024.126439.
    10.1016/j.seppur.2024.126439
  2. Malik Abdul Wahab; C. Domingues; Azevedo, A.M.; Virginia Chu; J.P. Conde; M. Raquel Aires-Barros. "An integrated microfluidic device for continuous bioprocessing". Separation and Purification Technology (2024): http://dx.doi.org/10.1016/j.seppur.2023.125702.
    10.1016/j.seppur.2023.125702
  3. Ricardo Fradique; Andreia Jardim; Ana M Azevedo; Virginia Chu; João P Conde; M Raquel Aires-Barros. "Continuous microfluidic platform combining cell lysis and protein extraction for screening overall process conditions". Journal of Chemical Technology & Biotechnology (2023): https://doi.org/10.1002/jctb.7564.
    10.1002/jctb.7564
  4. Capela, E.V.; Magnis, I.; Rufino, A.F.C.S.; Torres-Acosta, M.A.; Aires-Barros, M.R.; Coutinho, J.A.P.; Azevedo, A.M.; e Silva, F.A.; Freire, M.G.. "Using three-phase partitioning for the purification and recovery of antibodies from biological media". Separation and Purification Technology 316 (2023): http://www.scopus.com/inward/record.url?eid=2-s2.0-85152262424&partnerID=MN8TOARS.
    10.1016/j.seppur.2023.123823
  5. Jacinto, M.J.; Wagner, Alexandra; Sá, Inês M.; Patinha, David J.S.; Marrucho, Isabel M.; Gonçalves, João; Willson, Richard C.; Azevedo, A.M.; Aires-Barros, M.R.. "Screening polymeric ionic liquids for chromatography-based purification of bacteriophage M13". Separation and Purification Technology 257 (2021): 117906. http://dx.doi.org/10.1016/j.seppur.2020.117906.
    10.1016/j.seppur.2020.117906
  6. Roque, Ana Cecília Afonso; Pina, Ana Sofia; Azevedo, Ana Margarida; Aires-Barros, Raquel; Jungbauer, Alois; Di Profio, Gianluca; Heng, Jerry Y. Y.; Haigh, Jonathan; Ottens, Marcel. "Anything but Conventional Chromatography Approaches in Bioseparation". Biotechnology Journal 15 8 (2020): 1900274. http://dx.doi.org/10.1002/biot.201900274.
    10.1002/biot.201900274
  7. Ricardo Fradique; Ana M. Azevedo; Virginia Chu; João P. Conde; M. Raquel Aires-Barros. "Microfluidic platform for rapid screening of bacterial cell lysis". Journal of Chromatography A 1610 (2020): 460539-460539. https://doi.org/10.1016/j.chroma.2019.460539.
    10.1016/j.chroma.2019.460539
  8. Ferreira-Faria, D.; Aires-Barros, M.R.; Azevedo, A.M.. "Continuous aqueous two-phase extraction: From microfluidics to integrated biomanufacturing". Fluid Phase Equilibria 508 (2020): http://www.scopus.com/inward/record.url?eid=2-s2.0-85078093920&partnerID=MN8TOARS.
    10.1016/j.fluid.2019.112438
  9. Inês F. Pinto; Ruben R. G. Soares; Maria R. Aires-Barros; Virginia Chu; João P. Conde; Ana M. Azevedo. "Optimizing the Performance of Chromatographic Separations Using Microfluidics: Multiplexed and Quantitative Screening of Ligands and Target Molecules". Biotechnology Journal (2019): 1800593-1800593. https://doi.org/10.1002/biot.201800593.
    10.1002/biot.201800593
  10. Aires-Barros, R.; Azevedo, A.M.; Ferreira, G.N.M.. "Biochemical Engineering Science—Sustainable Processes and Economies". Biotechnology Journal 14 8 (2019): http://www.scopus.com/inward/record.url?eid=2-s2.0-85069840573&partnerID=MN8TOARS.
    10.1002/biot.201900276
  11. Rosa, S.A.S.L.; Wagner, A.; da Silva, C.L.; Aires-Barros, M.R.; Azevedo, A.M.; Dias-Cabral, A.C.. "Mobile-Phase Modulators as Salt Tolerance Enhancers in Phenylboronate Chromatography: Thermodynamic Evaluation of the Mechanisms Underlying the Adsorption of Monoclonal Antibodies". Biotechnology Journal 14 10 (2019): http://www.scopus.com/inward/record.url?eid=2-s2.0-85070666684&partnerID=MN8TOARS.
    10.1002/biot.201800586
  12. São Pedro, M.N.; Azevedo, A.M.; Aires-Barros, M.R.; Soares, R.R.G.. "Minimizing the Influence of Fluorescent Tags on IgG Partition in PEG–Salt Aqueous Two-Phase Systems for Rapid Screening Applications". Biotechnology Journal 14 8 (2019): http://www.scopus.com/inward/record.url?eid=2-s2.0-85069855349&partnerID=MN8TOARS.
    10.1002/biot.201800640
  13. Jacinto, M.J.; Trabuco, J.R.C.; Vu, B.V.; Garvey, G.; Khodadadi, M.; Azevedo, A.M.; Aires-Barros, M.R.; et al. "Correction: Enhancement of lateral flow assay performance by electromagnetic relocation of reporter particles (PLoS One (2018) 13:1 (e0186782) DOI: 10.1371/journal.pone.0186782)". PLoS ONE 14 3 (2019): http://www.scopus.com/inward/record.url?eid=2-s2.0-85062878241&partnerID=MN8TOARS.
    10.1371/journal.pone.0213859
  14. Capela, E.V.; Santiago, A.E.; Rufino, A.F.C.S.; Tavares, A.P.M.; Pereira, M.M.; Mohamadou, A.; Aires-Barros, M.R.; et al. "Sustainable strategies based on glycine-betaine analogue ionic liquids for the recovery of monoclonal antibodies from cell culture supernatants". Green Chemistry 21 20 (2019): 5671-5682. http://www.scopus.com/inward/record.url?eid=2-s2.0-85073594334&partnerID=MN8TOARS.
    10.1039/c9gc02733e
  15. Sara A.S.L. Rosa; C.L. da Silva; M. Raquel Aires-Barros; A.C. Dias-Cabral; Ana M. Azevedo. "Thermodynamics of the adsorption of monoclonal antibodies in phenylboronate chromatography: Affinity versus multimodal interactions". Journal of Chromatography A (2018): https://doi.org/10.1016/j.chroma.2018.07.050.
    10.1016/j.chroma.2018.07.050
  16. Ruben R. G. Soares; Denis R. Santos; Inês F. Pinto; Ana M. Azevedo; M. Raquel Aires-Barros; Virginia Chu; João P. Conde. "Multiplexed microfluidic fluorescence immunoassay with photodiode array signal acquisition for sub-minute and point-of-need detection of mycotoxins". Lab on a Chip (2018): https://doi.org/10.1039/C8LC00259B.
    10.1039/C8LC00259B
  17. Pinto, I.F.; Santos, D.R.; Soares, R.R.G.; Aires-Barros, M.R.; Chu, V.; Azevedo, A.M.; Conde, J.P.. "A regenerable microfluidic device with integrated valves and thin-film photodiodes for rapid optimization of chromatography conditions". Sensors and Actuators, B: Chemical 255 (2018): 3636-3646. http://www.scopus.com/inward/record.url?eid=2-s2.0-85030467065&partnerID=MN8TOARS.
    10.1016/j.snb.2017.09.167
  18. Ruben R. G. Soares; Alessandra Ricelli; Corrado Fanelli; Domenico Caputo; Giampiero de Cesare; Virginia Chu; M. Raquel Aires-Barros; João P. Conde. "Advances, challenges and opportunities for point-of-need screening of mycotoxins in foods and feeds". The Analyst (2018): https://doi.org/10.1039/C7AN01762F.
    10.1039/C7AN01762F
  19. Campos-Pinto, I.; Capela, E.V.; Silva-Santos, A.R.; Rodríguez, M.A.; Gavara, P.R.; Fernandez-Lahore, M.; Aires-Barros, M.R.; Azevedo, A.M.. "LYTAG-driven purification strategies for monoclonal antibodies using quaternary amine ligands as affinity matrices". Journal of Chemical Technology and Biotechnology 93 7 (2018): 1966-1974. http://www.scopus.com/inward/record.url?eid=2-s2.0-85048536549&partnerID=MN8TOARS.
    10.1002/jctb.5460
  20. Nascimento, A.; Pinto, I.F.; Chu, V.; Aires-Barros, M.R.; Conde, J.P.; Azevedo, A.M.. "Studies on the purification of antibody fragments". Separation and Purification Technology 195 (2018): 388-397. http://www.scopus.com/inward/record.url?eid=2-s2.0-85038821229&partnerID=MN8TOARS.
    10.1016/j.seppur.2017.12.033
  21. Jacinto, M.J.; Trabuco, J.R.C.; Vu, B.V.; Garvey, G.; Khodadady, M.; Azevedo, A.M.; Aires-Barros, M.R.; et al. "Enhancement of lateral flow assay performance by electromagnetic relocation of reporter particles". PLoS ONE 13 1 (2018): http://www.scopus.com/inward/record.url?eid=2-s2.0-85040368484&partnerID=MN8TOARS.
    10.1371/journal.pone.0186782
  22. Jacinto, M.J.; Patinha, D.J.S.; Marrucho, I.M.; Gonçalves, J.; Willson, R.C.; Azevedo, A.M.; Aires-Barros, M.R.. "M13 bacteriophage purification using poly(ionic liquids) as alternative separation matrices". Journal of Chromatography A 1532 (2018): 246-250. http://www.scopus.com/inward/record.url?eid=2-s2.0-85038622459&partnerID=MN8TOARS.
    10.1016/j.chroma.2017.12.005
  23. Bras, E.J.S.; Soares, R.R.G.; Azevedo, A.M.; Fernandes, P.; Arévalo-Rodríguez, M.; Chu, V.; Conde, J.P.; Aires-Barros, M.R.. "A multiplexed microfluidic toolbox for the rapid optimization of affinity-driven partition in aqueous two phase systems". Journal of Chromatography A 1515 (2017): 252-259. http://www.scopus.com/inward/record.url?eid=2-s2.0-85028061734&partnerID=MN8TOARS.
    10.1016/j.chroma.2017.07.094
  24. Silva, D.F.C.; Azevedo, A.M.; Fernandes, P.; Chu, V.; Conde, J.P.; Aires-Barros, M.R.. "Determination of partition coefficients of biomolecules in a microfluidic aqueous two phase system platform using fluorescence microscopy". Journal of Chromatography A 1487 (2017): 242-247. http://www.scopus.com/inward/record.url?eid=2-s2.0-85009737089&partnerID=MN8TOARS.
    10.1016/j.chroma.2016.12.036
  25. Campos-Pinto, I.; Espitia-Saloma, E.; Rosa, S.A.S.L.; Rito-Palomares, M.; Aguilar, O.; Arévalo-Rodríguez, M.; Aires-Barros, M.R.; Azevedo, A.M.. "Integration of cell harvest with affinity-enhanced purification of monoclonal antibodies using aqueous two-phase systems with a dual tag ligand". Separation and Purification Technology 173 (2017): 129-134. http://www.scopus.com/inward/record.url?eid=2-s2.0-84988410976&partnerID=MN8TOARS.
    10.1016/j.seppur.2016.09.017
  26. Grilo, A.L.; Mateus, M.; Aires-Barros, M.R.; Azevedo, A.M.. "Monoclonal Antibodies Production Platforms: An Opportunity Study of a Non-Protein-A Chromatographic Platform Based on Process Economics". Biotechnology Journal 12 12 (2017): http://www.scopus.com/inward/record.url?eid=2-s2.0-85038386195&partnerID=MN8TOARS.
    10.1002/biot.201700260
  27. Soares, R.R.G.; Azevedo, A.M.; Fernandes, P.; Chu, V.; Conde, J.P.; Aires-Barros, M.R.. "A simple method for point-of-need extraction, concentration and rapid multi-mycotoxin immunodetection in feeds using aqueous two-phase systems". Journal of Chromatography A 1511 (2017): 15-24. http://www.scopus.com/inward/record.url?eid=2-s2.0-85021980127&partnerID=MN8TOARS.
    10.1016/j.chroma.2017.07.004
  28. Brás, E.J.S.; Chu, V.; Aires-Barros, M.R.; Conde, J.P.; Fernandes, P.. "A microfluidic platform for physical entrapment of yeast cells with continuous production of invertase". Journal of Chemical Technology and Biotechnology 92 2 (2017): 334-341. http://www.scopus.com/inward/record.url?eid=2-s2.0-84969920412&partnerID=MN8TOARS.
    10.1002/jctb.5010
  29. Soares, R.R.G.; Santos, D.R.; Chu, V.; Azevedo, A.M.; Aires-Barros, M.R.; Conde, J.P.. "A point-of-use microfluidic device with integrated photodetector array for immunoassay multiplexing: Detection of a panel of mycotoxins in multiple samples". Biosensors and Bioelectronics 87 (2017): 823-831. http://www.scopus.com/inward/record.url?eid=2-s2.0-84988037419&partnerID=MN8TOARS.
    10.1016/j.bios.2016.09.041
  30. Pinto, I.F.; Caneira, C.R.F.; Soares, R.R.G.; Madaboosi, N.; Aires-Barros, M.R.; Conde, J.P.; Azevedo, A.M.; Chu, V.. "The application of microbeads to microfluidic systems for enhanced detection and purification of biomolecules". Methods 116 (2017): 112-124. http://www.scopus.com/inward/record.url?eid=2-s2.0-85007566334&partnerID=MN8TOARS.
    10.1016/j.ymeth.2016.12.005
  31. Pinto, I.F.; Rosa, S.A.S.L.; Aires-Barros, M.R.; Azevedo, A.M.. "Exploring the use of heparin as a first capture step in the purification of monoclonal antibodies from cell culture supernatants". Biochemical Engineering Journal 104 (2017): http://www.scopus.com/inward/record.url?eid=2-s2.0-84929646115&partnerID=MN8TOARS.
    10.1016/j.bej.2015.05.011
  32. Aires-Barros, R.; Büchs, J.; Ferreira, G.. "Editorial: ECAB focus issue: Engineered catalysts, robust, cost-effective and integrated bioprocesses and high-throughput screening". Biotechnology Journal 11 12 (2016): 1475-1476. http://www.scopus.com/inward/record.url?eid=2-s2.0-85003875984&partnerID=MN8TOARS.
    10.1002/biot.201600662
  33. Grilo, A.L.; Aires-Barros, M.R.; Azevedo, A.M.. "Partitioning in Aqueous Two-Phase Systems: Fundamentals, Applications and Trends". Separation and Purification Reviews 45 1 (2016): 68-80. http://www.scopus.com/inward/record.url?eid=2-s2.0-84945903068&partnerID=MN8TOARS.
    10.1080/15422119.2014.983128
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  92. Neves-Petersen, M.T.; Klitgaard, S.; Carvalho, A.S.L.; Petersen, S.B.; De Barros, M.R.A.; E Meloz, E.P.. "Photophysics and photochemistry of horseradish peroxidase A2 upon ultraviolet illumination". Biophysical Journal 92 6 (2007): 2016-2027. http://www.scopus.com/inward/record.url?eid=2-s2.0-33947658503&partnerID=MN8TOARS.
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  93. Azevedo, A.M.; Rosa, P.A.J.; Ferreira, I.F.; Aires-Barros, M.R.. "Optimisation of aqueous two-phase extraction of human antibodies". Journal of Biotechnology 132 2 (2007): 209-217. http://www.scopus.com/inward/record.url?eid=2-s2.0-35348995017&partnerID=MN8TOARS.
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  94. Costa, M.J.L.; Pedro, L.; De Matos, A.P.A.; Aires-Barros, M.R.; Belo, J.A.; Goncalves, J.; Ferreira, G.N.M.. "Molecular construction of bionanoparticles: Chimaeric SIV p17-HIV 1 p6 nanoparticles with minimal viral protein content". Biotechnology and Applied Biochemistry 48 1 (2007): 35-43. http://www.scopus.com/inward/record.url?eid=2-s2.0-34548527272&partnerID=MN8TOARS.
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  95. Rosa, P.A.J.; Azevedo, A.M.; Ferreira, I.F.; de Vries, J.; Korporaal, R.; Verhoef, H.J.; Visser, T.J.; Aires-Barros, M.R.. "Affinity partitioning of human antibodies in aqueous two-phase systems". Journal of Chromatography A 1162 1 SPEC. IS (2007): 103-113. http://www.scopus.com/inward/record.url?eid=2-s2.0-34547229213&partnerID=MN8TOARS.
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  96. Regado, M.A.; Cristóvão, B.M.; Moutinho, C.G.; Balcão, V.M.; Aires-Barros, R.; Ferreira, J.P.M.; Malcata, F.X.. "Flavour development via lipolysis of milkfats: Changes in free fatty acid pool". International Journal of Food Science and Technology 42 8 (2007): 961-968. http://www.scopus.com/inward/record.url?eid=2-s2.0-34547179414&partnerID=MN8TOARS.
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  97. Carvalho, A.S.L.; Ferreira, B.S.; Neves-Petersen, M.T.; Petersen, S.B.; Aires-Barros, M.R.; Melo, E.P.. "Thermal denaturation of HRPA2: pH-dependent conformational changes". Enzyme and Microbial Technology 40 4 (2007): 696-703. http://www.scopus.com/inward/record.url?eid=2-s2.0-33847263892&partnerID=MN8TOARS.
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  99. Madeira, C.; Loura, L.M.S.; Prieto, M.; Fedorov, A.; Aires-Barros, M.R.. "Liposome complexation efficiency monitored by FRET: Effect of charge ratio, helper lipid and plasmid size". European Biophysics Journal 36 6 (2007): 609-620. http://www.scopus.com/inward/record.url?eid=2-s2.0-34347399346&partnerID=MN8TOARS.
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  100. Madeira, C.; Fedorov, A.; Aires-Barros, M.R.; Prieto, M.; Loura, L.M.S.. "Photophysical behavior of a dimeric cyanine dye (BOBO-1) within cationic liposomes". Photochemistry and Photobiology 81 6 (2005): 1450-1459. http://www.scopus.com/inward/record.url?eid=2-s2.0-29844457440&partnerID=MN8TOARS.
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  101. Carvalho, A.S.L.; Neves-Petersen, M.T.; Petersen, S.B.; Aires-Barros, M.R.; Pinho E Melo, E.. "Formation of a misfolded conformation during refolding of HRPA1 in the presence of calcium". Biochimica et Biophysica Acta - Proteins and Proteomics 1747 1 (2005): 99-107. http://www.scopus.com/inward/record.url?eid=2-s2.0-12844249413&partnerID=MN8TOARS.
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  102. Carvalho, A.S.L.; Santos, A.M.; Neves-Petersen, M.T.; Petersen, S.B.; Aires-Barros, M.R.; Pinho E Melo, E.. "Conformational states of HRPA1 induced by thermal unfolding: Effect of low molecular weight solutes". Biopolymers 75 2 (2004): 173-186. http://www.scopus.com/inward/record.url?eid=2-s2.0-4944228100&partnerID=MN8TOARS.
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  103. Serralha, F.N.; Lopes, J.M.; Lemos, F.; Ramôa Ribeiro, F.; Prazeres, D.M.F.; Aires-Barros, M.R.; Cabral, J.M.S.. "Application of factorial design to the study of an alcoholysis transformation promoted by cutinase immobilized on NaY zeolite and Accurel PA6". Journal of Molecular Catalysis B: Enzymatic 27 1 (2004): 19-27. http://www.scopus.com/inward/record.url?eid=2-s2.0-0346725817&partnerID=MN8TOARS.
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  104. Sá-Pereira, P.; Carvalho, A.S.L.; Costa-Ferreira, M.; Aires-Barros, M.R.. "Thermostabilization of Bacillus subtillis CCMI 966 xylanases with trehalose: Study of deactivation kinetics". Enzyme and Microbial Technology 34 3-4 (2004): 278-282. http://www.scopus.com/inward/record.url?eid=2-s2.0-0442307777&partnerID=MN8TOARS.
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  105. Gonçalves, A.M.D.; Aires-Barros, M.R.; Cabral, J.M.S.. "Interaction of an anionic surfactant with a recombinant cutinase from Fusarium solani pisi: A spectroscopic study". Enzyme and Microbial Technology 32 7 (2003): 868-879. http://www.scopus.com/inward/record.url?eid=2-s2.0-17644440298&partnerID=MN8TOARS.
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  106. Cunha, M.T.; Costa, M.J.L.; Calado, C.R.C.; Fonseca, L.P.; Aires-Barros, M.R.; Cabral, J.M.S.. "Integration of production and aqueous two-phase systems extraction of extracellular Fusarium solani pisi cutinase fusion proteins". Journal of Biotechnology 100 1 (2003): 55-64. http://www.scopus.com/inward/record.url?eid=2-s2.0-0037426677&partnerID=MN8TOARS.
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  107. Madeira, C.; Loura, L.M.S.; Aires-Barros, M.R.; Fedorov, A.; Prieto, M.. "Characterization of DNA/Lipid Complexes by Fluorescence Resonance Energy Transfer". Biophysical Journal 85 5 (2003): 3106-3119. http://www.scopus.com/inward/record.url?eid=2-s2.0-0242353819&partnerID=MN8TOARS.
  108. Carvalho, A.S.L.; Melo, E.P.E.; Ferreira, B.S.; Neves-Petersen, M.T.; Petersen, S.B.; Aires-Barros, M.R.. "Heme and pH-dependent stability of an anionic horseradish peroxidase". Archives of Biochemistry and Biophysics 415 2 (2003): 257-267. http://www.scopus.com/inward/record.url?eid=2-s2.0-0038385478&partnerID=MN8TOARS.
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  109. Sá-Pereira, P.; Paveia, H.; Costa-Ferreira, M.; Aires-Barros, M.R.. "A new look at xylanases: An overview of purification strategies". Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology 24 3 (2003): 257-281. http://www.scopus.com/inward/record.url?eid=2-s2.0-0038746840&partnerID=MN8TOARS.
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  110. Sá-Pereira, P.; Mesquita, A.; Duarte, J.C.; Barros, M.R.A.; Costa-Ferreira, M.. "Rapid production of thermostable cellulase-free xylanase by a strain of Bacillus subtilis and its properties". Enzyme and Microbial Technology 30 7 (2002): 924-933. http://www.scopus.com/inward/record.url?eid=2-s2.0-0037018888&partnerID=MN8TOARS.
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  111. Serralha, F.N.; Lopes, J.M.; Aires-Barros, M.R.; Prazeres, D.M.F.; Cabral, J.M.S.; Lemos, F.; Ramôa Ribeiro, F.. "Stability of a recombinant cutinase immobilized on zeolites". Enzyme and Microbial Technology 31 1-2 (2002): 29-34. http://www.scopus.com/inward/record.url?eid=2-s2.0-0036644004&partnerID=MN8TOARS.
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  112. Pedro, A.J.E.; Fevereiro, P.S.; Serralheiro, M.L.; Aires-Barros, M.R.. "Study of the stability of Vaccinium myrtillus peroxidase in reverse micellar systems". Biocatalysis and Biotransformation 20 2 (2002): 129-135. http://www.scopus.com/inward/record.url?eid=2-s2.0-0036222562&partnerID=MN8TOARS.
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  113. Sá-Pereira, P.; Costa-Ferreira, M.; Aires-Barros, M.R.. "Enzymatic properties of a neutral endo-1,3(4)-ß-xylanase Xyl II from Bacillus subtilis". Journal of Biotechnology 94 3 (2002): 265-275. http://www.scopus.com/inward/record.url?eid=2-s2.0-0037061562&partnerID=MN8TOARS.
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  114. Cunha, T.; Aires-Barros, R.. "Large-scale extraction of proteins". Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology 20 1 (2002): 29-40. http://www.scopus.com/inward/record.url?eid=2-s2.0-0036161197&partnerID=MN8TOARS.
  115. Cristina Oliveira, A.; Fernanda Rosa, M.; Cabral, J.M.S.; Raquel Aires-Barros, M.. "Effect of extraction and enzymatic esterification of ethanol on glucose consumption by two Saccharomyces cerevisiae strains: A comparative study". Journal of Chemical Technology and Biotechnology 76 3 (2001): 285-290. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035119408&partnerID=MN8TOARS.
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  116. Stazi, S.R.; Fevereiro, P.; Sermanni, G.G.; Barros, R.A.. "Oxidative polymerization and partial dechlorination of 2,4,6-trichlorophenol by a mixture of peroxidase isozymes from Vaccinium myrtillus". Journal of Chemical Technology and Biotechnology 76 2 (2001): 210-214. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035143788&partnerID=MN8TOARS.
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  117. Serralha, F.N.; Lopes, J.M.; Lemos, F.; Prazeres, D.M.F.; Aires-Barros, M.R.; Cabral, J.M.S.; Ramôa Ribeiro, F.. "Kinetics and modelling of an alcoholysis reaction catalyzed by cutinase immobilized on NaY zeolite". Journal of Molecular Catalysis - B Enzymatic 11 4-6 (2001): 713-718. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035931488&partnerID=MN8TOARS.
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  118. Oliveira, A.C.; Rosa, M.F.; Aires-Barros, M.R.; Cabral, J.M.S.. "Enzymatic esterification of ethanol and oleic acid - A kinetic study". Journal of Molecular Catalysis - B Enzymatic 11 4-6 (2001): 999-1005. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035931385&partnerID=MN8TOARS.
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  119. Carvalho, C.M.L.; Aires-Barros, M.R.; Cabral, J.M.S.. "A continuous membrane bioreactor for ester synthesis in organic media: II. Modeling of MBR continuous operation". Biotechnology and Bioengineering 72 2 (2001): 136-143. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035819405&partnerID=MN8TOARS.
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  121. Serralha, F.N.; Lopes, J.M.; Vieira Ferreira, L.F.; Lemos, F.; Prazeres, D.M.F.; Aires-Barros, M.R.; Cabral, J.M.S.; Ramôa Ribeiro, F.. "Conformational changes induced by immobilization of a recombinant cutinase on zeolites". Catalysis Letters 73 1 (2001): 63-66. http://www.scopus.com/inward/record.url?eid=2-s2.0-0038227096&partnerID=MN8TOARS.
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  122. CARVALHO, CML; AIRES-BARROS, MR; CABRAL, JMS. "An integrated model for enzymatic reactions in reverse micellar systems: Nominal and effective substrate concentrations". Langmuir 16 7 (2000): 3082-3092.
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  123. Teresa Cunha, M.; Cabral, J.M.S.; Tjerneld, F.; Raquel Aires-Barros, M.. "Effect of salts and surfactants on the partitioning of fusarium solani pisi cutinase in aqueous two-phase systems of thermoseparating ethylene oxide/propylene oxide random copolymer and hydroxypropyl starch". Bioseparation 9 4 (2000): 203-209. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034468013&partnerID=MN8TOARS.
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  124. Oliveira, A.C.; Rosa, M.F.; Aires-Barros, M.R.; Cabral, J.M.S.. "Enzymatic esterification of ethanol by an immobilised Rhizomucor miehei lipase in a perforated rotating disc bioreactor". Enzyme and Microbial Technology 26 5-6 (2000): 446-450. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034003072&partnerID=MN8TOARS.
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  125. Porto, A.L.F.; Sarubbo, L.A.; Lima-Filho, J.L.; Aires-Barros, M.R.; Cabral, J.M.S.; Tambourgi, E.B.. "Hydrodynamics and mass transfer in aqueous two-phase protein extraction using a continuous perforated rotating disc contactor". Bioprocess Engineering 22 3 (2000): 215-218. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034076969&partnerID=MN8TOARS.
  126. Carvalho, C.M.L.; Cunnah, P.; Aires-Barros, M.R.; Cabral, J.M.S.. "Performance of a membrane bioreactor for enzymatic transesterification: Characterization and comparison with a batch stirred tank reactor". Biocatalysis and Biotransformation 18 1 (2000): 31-57. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034001597&partnerID=MN8TOARS.
  127. Serralha, F.N.; Lopes, J.M.; Lemos, F.; Prazeres, D.; Aires-Barros, M.; Rocha, J.; Cabral, J.; Ribeiro, F.R.. "Titanosilicates as supports for an enzymatic alcoholysis reaction". Reaction Kinetics and Catalysis Letters 69 2 (2000): 217-222. http://www.scopus.com/inward/record.url?eid=2-s2.0-0007679927&partnerID=MN8TOARS.
  128. Costa, M.J.L.; Cunha, M.T.; Cabral, J.M.S.; Aires-Barros, M.R.. "Scale-up of recombinant cutinase recovery by whole broth extraction with PEG-phosphate aqueous two-phase". Bioseparation 9 4 (2000): 231-238. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034468230&partnerID=MN8TOARS.
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  129. Carvalho, C.M.L.; Aires-Barros, M.R.; Cabral, J.M.S.. "Kinetics of cutinase catalyzed transesterification in AOT reversed micelles: Modeling of a batch stirred tank reactor". Journal of Biotechnology 81 1 (2000): 1-13. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034725958&partnerID=MN8TOARS.
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  130. Gonçalves, A.M.; Serro, A.P.; Aires-Barros, M.R.; Cabral, J.M.S.. "Effects of ionic surfactants used in reversed micelles on cutinase activity and stability". Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology 1480 1-2 (2000): 92-106. http://www.scopus.com/inward/record.url?eid=2-s2.0-0343953095&partnerID=MN8TOARS.
    10.1016/S0167-4838(00)00093-5
  131. Gonçalves, A.M.; Schacht, E.; Matthijs, G.; Aires Barros, M.R.; Cabral, J.M.S.; Gil, M.H.. "Stability studies of a recombinant cutinase immobilized to dextran and derivatized silica supports". Enzyme and Microbial Technology 24 1-2 (1999): 60-66. http://www.scopus.com/inward/record.url?eid=2-s2.0-0342459772&partnerID=MN8TOARS.
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  132. Morna-Gomes, A.; Serralheiro, M.L.; Fevereiro, P.S.; Aires-Barros, M.R.. "Application of factorial design to the optimization of peroxidase activity in reverse micelles of bis(2-ethylhexyl)sodium sulfosuccinate/isooctane". Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology 82 1 (1999): 27-36. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033506592&partnerID=MN8TOARS.
  133. Carvalho, C.M.L.; Aires-Barros, M.R.; Cabral, J.M.S.. "Cutinase: From molecular level to bioprocess development". Biotechnology and Bioengineering 66 1 (1999): 17-34. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033230644&partnerID=MN8TOARS.
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  134. Carvalho, C.M.L.; Cabral, J.M.S.; Aires-Barros, M.R.. "Cutinase stability in AOT reversed micelles: System optimization using the factorial design methodology". Enzyme and Microbial Technology 24 8-9 (1999): 569-576. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032920502&partnerID=MN8TOARS.
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  135. Krieger, N.; Taipa, M.A.; Melo, E.H.M.; Lima-Filho, J.L.; Aires-Barros, M.R.; Cabral, J.M.S.. "Purification of a Penicillium citrinum lipase by chromatographic processes". Bioprocess Engineering 20 1 (1999): 59-65. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033003342&partnerID=MN8TOARS.
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  136. Krieger, N.; Taipa, M.A.; Melo, E.H.M.; Lima-Filho, J.L.; Aires-Barros, M.R.; Cabral, J.M.S.. "Purification of a Penicillium citrinum lipase by chromatographic processes". Bioprocess Engineering 20 1 (1999): 59-65. http://www.scopus.com/inward/record.url?eid=2-s2.0-85047676521&partnerID=MN8TOARS.
    10.1007/PL00009034
  137. Carvalho, C.M.L.; Melo, E.P.; Costa, S.M.B.; Cabral, J.M.S.; Aires-Barros, M.R.. "A steady-state fluorescence study of cutinase microencapsulated in AOT reversed micelles at optimal stability conditions". Biotechnology Letters 21 8 (1999): 673-681. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032830485&partnerID=MN8TOARS.
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  138. Fontes, N.; Conceição Almeida, M.; Peres, C.; Garcia, S.; Grave, J.; Aires-Barros, M.R.; Soares, C.M.; et al. "Cutinase activity and enantioselectivity in supercritical fluids". Industrial and Engineering Chemistry Research 37 8 (1998): 3189-3194. http://www.scopus.com/inward/record.url?eid=2-s2.0-3242794546&partnerID=MN8TOARS.
    10.1021/ie970933c
  139. Cunha, M.T.; Tjerneld, F.; Cabral, J.M.S.; Maria Raquel Aires-Barros. "Effect of electrolytes and surfactants on the thermoseparation of an ethylene oxide-propylene oxide random copolymer in aqueous solution". Journal of Chromatography B: Biomedical Applications 711 1-2 (1998): 53-60. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032569067&partnerID=MN8TOARS.
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  140. Carvalho, C.M.L.; Aires-Barros, M.R.; Cabral, J.M.S.. "Cutinase structure, function and biocatalytic applications". Electronic Journal of Biotechnology 1 3 (1998): 91-113. http://www.scopus.com/inward/record.url?eid=2-s2.0-0343024606&partnerID=MN8TOARS.
  141. Castellar, M.R.; Aires-Barros, M.R.; Cabral, J.M.S.; Iborra, J.L.. "Effect of zeolite addition on ethanol production from glucose by Saccharomyces bayanus". Journal of Chemical Technology and Biotechnology 73 4 (1998): 377-384. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032401176&partnerID=MN8TOARS.
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  142. Serralha, F.N.; Lopes, J.M.; Lemos, F.; Prazeres, D.M.F.; Aires-Barros, M.R.; Cabral, J.M.S.; Ramôa Ribeiro, F.. "Zeolites as supports for an enzymatic alcoholysis reaction". Journal of Molecular Catalysis - B Enzymatic 4 5-6 (1998): 303-311. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032479932&partnerID=MN8TOARS.
    10.1016/S1381-1177(98)00069-1
  143. Almeida, M.C.; Venâncio, A.; Teixeira, J.A.; Aires-Barros, M.R.. "Cutinase purification on poly(ethylene glycol)-hydroxypropyl starch aqueous two-phase systems". Journal of Chromatography B: Biomedical Applications 711 1-2 (1998): 151-159. http://www.scopus.com/inward/record.url?eid=2-s2.0-0344541813&partnerID=MN8TOARS.
    10.1016/S0378-4347(97)00680-4
  144. Melo, E.P.; Carvalho, C.M.L.; Aires-Barros, M.R.; Costa, S.M.B.; Cabral, J.M.S.. "Deactivation and conformational changes of cutinase in reverse micelles". Biotechnology and Bioengineering 58 4 (1998): 380-386. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032550831&partnerID=MN8TOARS.
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  145. Carvalho, C.M.L.; Cabral, J.M.S.; Aires-Barros, M.R.. "Kinetics and modelling of transesterification reactions catalysed by cutinase in AOT reversed micelles". Journal of Molecular Catalysis - B Enzymatic 5 1-4 (1998): 361-365. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032530874&partnerID=MN8TOARS.
    10.1016/S1381-1177(98)00098-8
  146. Gonçalves, A.P.V.; Cabral, J.M.S.; Aires-Barros, M.R.. "Analysis of a BSTR reactor for triglyceride hydrolysis with an immobilised cutinase". Journal of Molecular Catalysis - B Enzymatic 5 1-4 (1998): 35-38. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032530506&partnerID=MN8TOARS.
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  147. Oliveira, A.C.; Rosa, M.F.; Cabral, J.M.S.; Aires-Barros, M.R.. "Improvement of alcoholic fermentations by simultaneous extraction and enzymatic esterification of ethanol". Journal of Molecular Catalysis - B Enzymatic 5 1-4 (1998): 29-33. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032530418&partnerID=MN8TOARS.
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  148. Oliveira, A.C.; Rosa, M.F.; Cabral, J.M.S.; Aires-Barros, M.R.. "Immobilization of saccharomyces cerevisiae cells and rhizomocur miehei lipase for the production and extractive biocatalysis of ethanol". Bioprocess Engineering 16 6 (1997): 349-353. http://www.scopus.com/inward/record.url?eid=2-s2.0-85047676658&partnerID=MN8TOARS.
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  149. Sebastião, M.J.; Martel, P.; Baptista, A.; Petersen, S.B.; Cabral, J.M.S.; Aires-Barros, M.R.. "Predicting the partition coefficients of a recombinant cutinase in polyethylene glycol/phosphate aqueous two-phase systems". Biotechnology and Bioengineering 56 3 (1997): 248-257. http://www.scopus.com/inward/record.url?eid=2-s2.0-0031555165&partnerID=MN8TOARS.
    10.1002/(SICI)1097-0290(19971105)56:3<248::AID-BIT2>3.0.CO;2-J
  150. Porto, A.L.F.; Lima-Filho, J.L.; Aires-Barros, M.R.; Cabral, J.M.S.; Tambourgi, E.B.. "Extraction of recombinant cytochrome b5 from disrupted Escherichia coli cells with an aqueous two-phase system in a continuous perforated rotating disc contactor". Biotechnology Techniques 11 9 (1997): 641-643. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030874790&partnerID=MN8TOARS.
  151. Sarmento, M.J.; Pires, M.J.; Cabral, J.M.S.; Aires-Barros, M.R.. "Liquid-liquid extraction of a recombinant protein, cytochrome b5, from an impure extract using aqueous two-phase systems". Bioprocess Engineering 16 5 (1997): 295-297. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030919844&partnerID=MN8TOARS.
    10.1007/s004490050325
  152. Gonçalves, A.P.V.; Lopes, J.M.; Lemos, F.; Ramôa Ribeiro, F.; Prazeres, D.M.F.; Cabral, J.M.S.; Aires-Barros, M.R.. "Effect of the immobilization support on the hydrolytic activity of a cutinase from Fusarium solani pisi". Enzyme and Microbial Technology 20 2 (1997): 93-101. http://www.scopus.com/inward/record.url?eid=2-s2.0-0031081636&partnerID=MN8TOARS.
    10.1016/S0141-0229(96)00089-0
  153. Oliveira, A.C.; Rosa, M.F.; Cabral, J.M.S.; Aires-Barros, M.R.. "Immobilization of saccharomyces cerevisiae cells and rhizomocur miehei lipase for the production and extractive biocatalysis of ethanol". Bioprocess Engineering 16 6 (1997): 349-353. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030920502&partnerID=MN8TOARS.
    10.1007/s004490050334
  154. Carvalho, C.M.L.; Serralheiro, M.L.M.; Cabral, J.M.S.; Aires-Barros, M.R.. "Application of factorial design to the study of transesterification reactions using cutinase in AOT-reversed micelles". Enzyme and Microbial Technology 21 2 (1997): 117-123. http://www.scopus.com/inward/record.url?eid=2-s2.0-0031214893&partnerID=MN8TOARS.
    10.1016/S0141-0229(96)00245-1
  155. Cunha, M.T.; Cabral, J.M.S.; Aires-Barros, M.R.. "Quantification of phase composition in aqueous two-phase systems of Breox/Phosphate and Breox/Reppal PES 100 by isocratic HPLC". Biotechnology Techniques 11 5 (1997): 351-353. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030904839&partnerID=MN8TOARS.
  156. Krieger, N.; Taipa, M.A.; Aires-Barros, M.R.; Melo, E.H.M.; Lima-Filho, J.L.; Cabral, J.M.S.. "Purification of the Penicillium citrinum lipase using AOT reversed micelles". Journal of Chemical Technology and Biotechnology 69 1 (1997): 77-85. http://www.scopus.com/inward/record.url?eid=2-s2.0-0031148956&partnerID=MN8TOARS.
    10.1002/(SICI)1097-4660(199705)69:1<77::AID-JCTB666>3.0.CO;2-V
  157. Pinho Melo, E.; Aires-Barros, M.R.; Costa, S.M.B.; Cabral, J.M.S.. "Thermal unfolding of proteins at high pH range studied by UV absorbance". Journal of Biochemical and Biophysical Methods 34 1 (1997): 45-59. http://www.scopus.com/inward/record.url?eid=2-s2.0-0031047243&partnerID=MN8TOARS.
    10.1016/S0165-022X(97)01202-5
  158. Cabral, J.M.S.; Aires-Barros, M.R.; Pinheiro, H.; Prazeres, D.M.F.. "Biotransformation in organic media by enzymes and whole cells". Journal of Biotechnology 59 1-2 (1997): 133-143. http://www.scopus.com/inward/record.url?eid=2-s2.0-0031576709&partnerID=MN8TOARS.
    10.1016/S0168-1656(97)00176-4
  159. Krieger, N.; Taipa, M.A.; Melo, E.H.M.; Lima-Filho, J.L.; Aires-Barros, M.R.; Cabral, J.M.S.. "Kinetic Characterization of Penicillium citrinum Lipase in AOT/Isooctane-Reversed Micelles". Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology 67 1-2 (1997): 87-95. http://www.scopus.com/inward/record.url?eid=2-s2.0-1842817562&partnerID=MN8TOARS.
  160. Gonalves, A.P.V.; Cabral, J.M.S.; Aires-Barros, M.R.. "Immobilization of a recombinant cutinase by entrapment and by covalent binding kinetic and stability studies". Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology 60 3 (1996): 217-228. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030227450&partnerID=MN8TOARS.
  161. Cabral, J.M.S.; Aires-Barros, M.R.. "Biocatalysis in organic media by cutinase immobilized in reversed micelles and zeolites". Chimia 50 9 (1996): 420-421. http://www.scopus.com/inward/record.url?eid=2-s2.0-0042856687&partnerID=MN8TOARS.
  162. Cunnah, P.J.; Aires-Barros, M.R.; Cabral, J.M.S.. "Esterification and transesterification catalysed by cutinase in reverse micelles of CTAB for the synthesis of short chain esters". Biocatalysis and Biotransformation 14 2 (1996): 125-146. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030413045&partnerID=MN8TOARS.
  163. Pires, M.J.; Aires-Barros, M.R.; Cabral, J.M.S.. "Liquid-liquid extraction of proteins with reversed micelles". Biotechnology Progress 12 3 (1996): 290-301. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030144987&partnerID=MN8TOARS.
  164. Carneiro-da-Cunha, M.G.; Aires-Barros, M.R.; Tambourgi, E.B.; Cabral, J.M.S.. "Continuous extraction of a recombinant cutinase from Escherichia coli disrupted cells with reversed micelles using a perforated rotating disc contactor". Bioprocess Engineering 15 5 (1996): 253-256. http://www.scopus.com/inward/record.url?eid=2-s2.0-85047679639&partnerID=MN8TOARS.
    10.1007/BF02391586
  165. Gonçalves, A.P.V.; Lopes, J.M.; Lemos, F.; Ramôa Ribeiro, F.; Prazeres, D.M.F.; Cabrai, J.M.S.; Aires-Barros, M.R.. "Zeolites as supports for enzymatic hydrolysis reactions. Comparative study of several zeolites". Journal of Molecular Catalysis B: Enzymatic 1 2 (1996): 53-60. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030568516&partnerID=MN8TOARS.
    10.1016/1381-1177(95)00013-5
  166. Sebastião, M.J.; Cabral, J.M.S.; Aires-Barros, M.R.. "Improved purification protocol of a Fusarium solani pisi recombinant cutinase by phase partitioning in aqueous two-phase systems of polyethylene glycol and phosphate". Enzyme and Microbial Technology 18 4 (1996): 251-260. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030111019&partnerID=MN8TOARS.
    10.1016/0141-0229(95)00045-3
  167. Carneiro-da-Cunha, M.G.; Aires-Barros, M.R.; Tambourgi, E.B.; Cabral, J.M.S.. "Continuous extraction of a recombinant cutinase from Escherichia coli disrupted cells with reversed micelles using a perforated rotating disc contactor". Bioprocess Engineering 15 5 (1996): 253-256. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029796267&partnerID=MN8TOARS.
    10.1007/s004490050264
  168. Carneiro-da-Cunha, M.G.; Melo, E.P.; Cabral, J.M.S.; Aires-Barros, M.R.. "Liquid-liquid extraction of a recombinant cutinase from fermentation media with AOT reversed micelles". Bioprocess Engineering 15 3 (1996): 151-157. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029763705&partnerID=MN8TOARS.
    10.1007/s004490050249
  169. Carneiro-da-Cunha, M.G.; Melo, E.P.; Cabral, J.M.S.; Aires-Barros, M.R.. "Liquid-liquid extraction of a recombinant cutinase from fermentation media with AOT reversed micelles". Bioprocess Engineering 15 3 (1996): 151-157. http://www.scopus.com/inward/record.url?eid=2-s2.0-85047679161&partnerID=MN8TOARS.
    10.1007/BF00369619
  170. Pinto-Sousa, A.M.; Cabrai, J.M.S.; Aires-Barros, M.R.. "Stability of a Fusarium solani pisi recombinant cutinase in phosphatidylcholine reversed micelles". Biotechnology Letters 18 5 (1996): 583-586. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029951623&partnerID=MN8TOARS.
    10.1007/BF00140207
  171. Setti, L.; Fevereiro, P.; Melo, E.P.; Pifferi, P.G.; Cabral, J.M.S.; Aires-Barros, M.R.. "Superactivity of peroxidase solubilized in reversed micellar systems". Applied Biochemistry and Biotechnology 55 3 (1995): 207-218. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029436379&partnerID=MN8TOARS.
    10.1007/BF02786860
  172. Melo, E.P.; Ivanova, M.G.; Aires-Barros, M.R.; Cabral, J.M.S.; Verger, R.. "Glyceride Synthesis Catalyzed by Cutinase Using the Monomolecular Film Technique". Biochemistry 34 5 (1995): 1615-1621. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028947712&partnerID=MN8TOARS.
    10.1021/bi00005a018
  173. Sebastião, M.J.; Cabral, J.M.S.; Aires-Barros, M.R.. "Simultaneous quantification of polymer and salt in polyethylene glycol / phosphate aqueous two-phase systems by HPLC". Biotechnology Techniques 9 7 (1995): 503-508. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028833240&partnerID=MN8TOARS.
    10.1007/BF00159566
  174. Melo, E.P.; Airesbarros, M.R.; Cabral, J.M.S.. "Triglyceride hydrolysis and stability of a recombinant cutinase from Fusarium solani in AOT-iso-octane reversed micelles". Applied Biochemistry and Biotechnology 50 1 (1995): 45-56. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029205576&partnerID=MN8TOARS.
    10.1007/BF02788039
  175. Vicente, L.C.; Aires-Barros, R.; Empis, J.M.A.. "Stability and proteolytic activity of papain in reverse micellar and aqueous media: A kinetic and spectroscopic study". Journal of Chemical Technology and Biotechnology 60 3 (1994): 291-297. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028474144&partnerID=MN8TOARS.
    10.1002/jctb.280600310
  176. Carneiro-da-Cunha, M.G.; Cabral, J.M.S.; Aires-Barros, M.R.. "Studies on the extraction and back-extraction of a recombinant cutinase in a reversed micellar extraction process". Bioprocess Engineering 11 5 (1994): 203-208. http://www.scopus.com/inward/record.url?eid=2-s2.0-0027960584&partnerID=MN8TOARS.
    10.1007/BF00369631
  177. PINTOSOUSA, AMC; CABRAL, JMS; AIRESBARROS, MR. "ESTER SYNTHESIS BY A RECOMBINANT CUTINASE IN REVERSED MICELLES OF A NATURAL PHOSPHOLIPID". Biocatalysis 9 1-4 (1994): 169-179.
    10.3109/10242429408992118
  178. Sebastião, M.J.; Cabral, J.M.S.; Aires-Barros, M.R.. "Partitioning of recombinant Fusarium solani pisi cutinase in polyethylene glycol-aqueous salt solution two-phase systems". Journal of Chromatography A 668 1 (1994): 139-144. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028360440&partnerID=MN8TOARS.
    10.1016/0021-9673(94)80102-9
  179. Videira, M.; Aires-Barros, M.R.. "Liquid-liquid extraction of clavulanic acid using an aqueous two-phase system of polyethylene glycol and potassium phosphate". Journal of Chromatography A 668 1 (1994): 237-240. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028338428&partnerID=MN8TOARS.
  180. Sarmento, M.J.; Pires, M.J.; Cabral, J.M.S.; Aires-Barros, M.R.. "Liquid-liquid extraction of a recombinant protein, cytochrome b5, with aqueous two-phase systems of polyethylene glycol and potassium phosphate salts". Journal of Chromatography A 668 1 (1994): 117-120. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028305574&partnerID=MN8TOARS.
    10.1016/0021-9673(94)80099-5
  181. Carneiro-da-Cunha, M.G.; Cabral, J.M.S.; Aires-Barros, M.R.. "Studies on the extraction and back-extraction of a recombinant cutinase in a reversed micellar extraction process". Bioprocess Engineering 11 5 (1994): 203-208. http://www.scopus.com/inward/record.url?eid=2-s2.0-0027960584&partnerID=MN8TOARS.
    10.1007/s004490050070
  182. Carneiro da Cunha, M.G.; Aires-Barros, M.R.; Tambourgi, E.B.; Cabral, J.M.S.. "Recovery of a recombinant cutinase with reversed micelles in a continuous perforated rotating disc contactor". Biotechnology Techniques 8 6 (1994): 413-418. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028002457&partnerID=MN8TOARS.
  183. Sebastião, M.J.; Cabral, J.M.S.; Aires-Barros, M.R.. "Fusarium solani pisi recombinant cutinase partitioning in peg/potassium phosphate aqueous two-phase systems". Biotechnology Techniques 7 9 (1993): 631-634. http://www.scopus.com/inward/record.url?eid=2-s2.0-0000982658&partnerID=MN8TOARS.
    10.1007/BF00151860
  184. Sebastiao, M.J.; Cabral, J.M.S.; Aires-Barros, M.R.. "Synthesis of fatty acid esters by a recombinant cutinase in reversed micelles". Biotechnology and Bioengineering 42 3 (1993): 326-332. http://www.scopus.com/inward/record.url?eid=2-s2.0-0027627398&partnerID=MN8TOARS.
  185. Taipa, M.A.; Aires-Barros, M.R.; Cabral, J.M.S.. "Purification of lipases". Journal of Biotechnology 26 2-3 (1992): 111-142. http://www.scopus.com/inward/record.url?eid=2-s2.0-0026677176&partnerID=MN8TOARS.
    10.1016/0168-1656(92)90001-P
  186. Taipa, M.A.; Aires-Barros, M.R.; Cabral, J.M.S.. "Purification of lipases from Chromobacterium viscosum: Is there a high molecular weight lipase?". Biotechnology Techniques 6 6 (1992): 533-538. http://www.scopus.com/inward/record.url?eid=2-s2.0-51249162969&partnerID=MN8TOARS.
    10.1007/BF02447827
  187. Schlatmann, J.; Aires-Barros, M.R.; Cabral, J.M.S.. "Esterification of short chain organic acids with alcohols by a lipase microencapsulated in reversed micelles". Biocatalysis and Biotransformation 5 2 (1991): 137-144. http://www.scopus.com/inward/record.url?eid=2-s2.0-0001079180&partnerID=MN8TOARS.
    10.3109/10242429109014862
  188. Aires-Barros, M.R.; Cabral, J.M.S.. "Selective separation and purification of two lipases from chromobacterium viscosum using AOT reversed micelles". Biotechnology and Bioengineering 38 11 (1991): 1302-1307. http://www.scopus.com/inward/record.url?eid=2-s2.0-0026333653&partnerID=MN8TOARS.
    10.1002/bit.260381107
  189. Vicente, M.L.C.; Aires-Barros, M.R.; Cabral, J.M.S.. "Purification of Chromobacterium viscosum lipases using reversed micelles". Biotechnology Techniques 4 2 (1990): 137-142. http://www.scopus.com/inward/record.url?eid=2-s2.0-0000804329&partnerID=MN8TOARS.
    10.1007/BF00163288
  190. Aires-Barros, M.R.; Cabral, J.M.S.; Willson, R.C.; Hamel, J.-F.P.; Cooney, C.L.. "Esterification-coupled extraction of organic acids: Partition enhancement and underlying reaction and distribution equilibria". Biotechnology and Bioengineering 34 7 (1989): 909-915. http://www.scopus.com/inward/record.url?eid=2-s2.0-0024962536&partnerID=MN8TOARS.
    10.1002/bit.260340705
  191. Aires Barros, M.R.; Cabral, J.M.S.; Novais, J.M.. "Production of ethanol by immobilized Saccharomyces bayanus in an extractive fermentation system". Biotechnology and Bioengineering 29 9 (1987): 1097-1104. http://www.scopus.com/inward/record.url?eid=2-s2.0-0023365739&partnerID=MN8TOARS.
  192. COSTA, SMB; DEBARROS, MRA; CONDE, JP. "PORPHYRIN-QUINONE EXCITED-STATE INTERACTIONS IN REVERSED MICELLES". Journal of Photochemistry 28 2 (1985): 153-164.
    10.1016/0047-2670(85)87027-1
Activities

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2018/09/01 - Current Continuous Manufacturing of Biological Products using OFR
Supervisor
2019 - 2023 Miniaturized platform to capture bio-products
Supervisor
 Biotecnologia e Biociências (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2015 - 2020 Development of an integrated microfluidic platform for biomolecule production and purification
Supervisor
 Bioengenharia e Biociências (PhD)
Associação para o Desenvolvimento do Instituto Superior Técnico, Portugal
2014 - 2019 Integrative platforms for downstream processing and detection of viral particles
Supervisor
 Bioengenharia (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2014 - 2019 Microfluidic separation using aqueous two phase systems
Supervisor
 Biotecnologia e Biociências (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2013/09/01 - 2018/11/17 Screening of multimodal ligands for the capture and polishing of antibody streams: A microfluidic approach for rapid optimization of chromatography operating conditions
Co-supervisor
2013/09/01 - 2018/10/16 Development of an Integrated Microfluidic Mycotoxin Screening Chip for Point-Of-Need Food Safety Applications
Co-supervisor
2014 - 2016 Downstream processing of cell culture-derived animal viruses
Supervisor
 Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2013 - 2013 Integrated Extraction, Concentration and Quantification of Ochratoxin A in Wines using a Microfluidic Aqueous Two Phase Extraction coupled to a Fluorescence Linked Immunosorbent Assay
Co-supervisor
 Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2013 - 2013 LYTAG-driven Integrative Platform for Purification of Monoclonal Antibodies by Aqueous Two-phase Systems
Supervisor
 Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2012 - 2012 Purification of IgG from animal cell cultures using gum arabic coated magnetic particles
Supervisor
 Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2012 - 2012 Kinetic and spectroscopic characterization of a DyP-Type peroxidase from Bacillus subtillis
Supervisor
 Engenharia Biológica (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2011 - 2011 Antibody separation using aqueous two-phase systems in a microfluidic
Supervisor
 Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2011 - 2011 Concepção e desenvolvimento de um bloqueador de cimento ósseo
Supervisor
 Engenharia Biológica (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2011 - 2011 An Integrated Process for the Purification of Antibodies Based on Magnetic Particles and Aqueous Two-Phase Systems
Supervisor
 Engenharia Biológica (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2010 - 2010 Phenylboronic acid as ligand for multimodal chromatography
Supervisor
 Bioengenharia (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2010 - 2010 A Business Model for Congestion Charging
Co-supervisor
 Transportes (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2010 - 2010 Phosphorylation by Alkaline Phosphatase: its use in cascade reactions
Supervisor
 Engenharia Química (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2010 - 2010 Characterization of Chromatography Steps in the Downstream Process of a Monoclonal Antibody
Supervisor
 Engenharia Biológica (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2010 - 2010 Development of a strategy for the affinity magnetic fishing of the lectin Concanavalin brasiliensis
Supervisor
 Engenharia Farmacêutica (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2010 - 2010 Desenvolvimento de um sistema microfluídico integrado para purificação de anticorpos
Co-supervisor
 Engenharia Biológica (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2008 - 2008 Magnetic adsorbents for the purification of monoclonal antibodies
Supervisor
 Biotecnologia (PhD)
2007 - 2007 Biodesulfurization of Crude Oil by Whole Cells of Rhodococcus erythropolis
Supervisor
 Bioengenharia (PhD)
2006 - 2006 Production of Virus Like Particles for Molecular Therapy
Supervisor
 Biotecnologia (PhD)
2006 - 2006 Purification of Canavalia Brasiliensis Lectin (conbr) by Aqueous Two-phase Systems
Supervisor
 Biotecnologia (PhD)
2005 - 2005 Structural and Physical-chemical Characterization of Lipoplexes for Gene Therapy and Dna Vaccines
Supervisor
 Biotecnologia (PhD)
2005 - 2005 Purification of Monoclonal Antibodies Using Aqueous Two-phase Systems
Supervisor
 Biotecnologia (PhD)
2004 - 2004 Conformational Stability Studies of Two Anionic Horseradish Peroxidase Isoenzymes
Supervisor
 Biotecnologia (PhD)
2001 - 2001 Separation and Purification of a Recombinant Cutinase Using Aqueous Two-phase Systems
Supervisor
 Biotecnologia (PhD)
2000 - 2000 Síntese de Ésteres de Ácidos Gordos por Biocatálise Extractiva de Etanol, Obtido por Fermentação Alcoólica
Supervisor
 Biotecnologia (PhD)
1999 - 1999 Optimization of a Reversed Micellar System with Microencapsulated Cutinase: Stabilization, Kinetics and Operation in Reactors
Supervisor
 Biotecnologia (PhD)
1999 - 1999 Imobilização de uma Cutinase Recombinada no Zeólito Nay: Estudos Cinéticos e de Estabilidade
Supervisor
 Biotecnologia (PhD)
1997 - 1997 Purificação de Lipases Utilizando Sistemas de Duas Fases Aquosas
Supervisor
 Biotecnologia (PhD)