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Laura Cerqueira (LC) has held her PhD in Biomedical Eng. in 2012 at UMinho. During PhD, she integrated the Biofilm Group to develop methods for pathogens detection based on Nucleic Acid Mimics (NAMs). Particularly one, related with the detection of Helicobacter pylori in gastric biopsies, gained more attention by its innovative potential. The result of this research let her to co-found a start-up, Biomode, intended to market proprietary PNA-FISH kits. Since then, her scientific career is focused on translational research to answer industrial needs on pathogens control combining research at LEPABE (UPorto) and technology transfer. Her scientific activity dealt with FISH methods improvement for food and environmental industry applications and on the miniaturized devices for the quasi-real detection of bacteria (LEPABE and CEFT). These studies are still ongoing. In 2019, she decided to work full time at LEPABE to expand her research field. To raise funding to support her studies, she applied to a FCT project (POCI-01-0145-FEDER-029961; 2018-2022) where a method for Legionella pneumophila (LP) monitoring was developed in collaboration with INIAV (PT). This project was the basis for LC to demonstrate her research independence and leadership. LC is, from 2023, an Assistant Researcher at LEPABE with interdisciplinary research on microbiology, molecular biology and microfluidics and she’s exploring PNA-FISH as a spatial transcriptomic fluorescent technique to determine the ecology and functionality of biofilms. Also relevant is the fact that LC count already with a team working under her supervision in the fields that she aims to pursue: Spatial transcriptomics in biofilms (2022.11840.BD and 2024.00822.BDANA), biofilms matrix composition (e.Biofilm PhD scholarship) and miniaturized structures for cell entrapment using microfluidics (2022.14186.BD). At 2022 she was awarded with the high impact CBE Young Investigator award as a recognition of her work on the application of PNA-FISH based-methods for bacterial detection and localization in biofilms. She focused on writing an ERA Chair (Horizon) project intending to create a group of Excellence on Engineered Biofilms at LEPABE, and she is now working as the project manager. Additionally, she is taking part of other projects, such as the COST Action CA20110 exRNA, as member of the Management Committee. She is author of 30 publications in international peer-reviewed journals, 3 book chapters and 5 patents.
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Identification

Personal identification

Full name
Laura Isabel Macieira Cerqueira

Citation names

  • Cerqueira, Laura
  • Cerqueira, L.

Author identifiers

Ciência ID
EC12-5302-3A70
ORCID iD
0000-0001-8452-4749
Scopus Author Id
23977598300

Email addresses

  • lcerqueira@fe.up.pt (Professional)
  • laura.imcerqueira@gmail.com (Personal)

Websites

Knowledge fields

  • Natural sciences - Biological Sciences - Microbiology
  • Engineering and Technology - Industrial Biotechnology - Industrial Biotechnology
  • Engineering and Technology - Nano-technology
  • Engineering and Technology - Medical Engineering - Medical Laboratory Technology
  • Natural sciences - Biological Sciences - Molecular Biology
  • Agrarian Sciences - Agrarian and Food Biotechnology - Diagnostics

Languages

Language Speaking Reading Writing Listening Peer-review
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
French Elementary (A2) Intermediate (B1) Elementary (A2) Intermediate (B1)
Spanish; Castilian Elementary (A2) Intermediate (B1) Beginner (A1) Intermediate (B1)
Education
Degree Classification
2024/10 - 2024/12/06
Ongoing
 Building and Leading Research Teams – 3rd edition (Outros)
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2018/07/07
Concluded
 Curso Geral de Gestão / Executive Education in General Management (Pós-Graduação)
Universidade Católica Portuguesa Católica Porto Business School, Portugal
 18
2014/05 - 2015/01
Concluded
 Integration of microfluidics and fluorescence in situ hybridization (FISH) for the rapid identification of microorganisms in clinical samples (Post-doctoral research) SFRH/BPD/98525/2013 (Pós-doutoramento)
Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal
2013/11 - 2014/04
Concluded
 DNA mimics: Development and application of DNA mimics for the rapid identification of microorganisms (Post-doctoral research) PIC/IC/82815/2007 (Pós-doutoramento)
Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal
2012/06/06 - 2012/06/15
Concluded
 Financials for Technology Entrepreneurs (Outros)
Major in Finance
COTEC, Portugal
2012/05/21 - 2012/06/06
Concluded
 Management Control for SMEs - Analysis of Indicators (Outros)
Major in Finance
TecMinho Associação Universidade-Empresa para o Desenvolvimento, Portugal
2012/02/28
Concluded
 Engenharia Biomédica / Biomedical Engineering (Doutoramento)
Universidade do Minho Centro de Engenharia Biológica, Portugal
"Development and Evaluation of peptide Nucleic acid (PNA) probes for the rapid detection of clinically relevant microorganisms" (THESIS/DISSERTATION)
2010/10/01 - 2011/03/31
Concluded
 English Conversation Classes (Outros)
Major in English
International House Braga, Portugal
2010/05 - 2010/05/10
Concluded
 Methods of DNA analysis state of the art. (Pós-Graduação)
Major in Molecular Biology and Genetics
Universidade do Minho Instituto de Investigação em Ciências da Vida e Saúde, Portugal
2009/11/09 - 2010/01/20
Concluded
 Formação Pedagógica Inicial de Formadores /Initial Pedagogical formation of trainers (Curso Técnico Superior Profissional)
Major in Pedagogical formation
Nexus, Braga (Portugal), Portugal
 5/5
2008 - 2009
Concluded
 English Course A2+ (Curso Técnico Superior Profissional)
Universidade do Minho Instituto de Letras e Ciências Humanas, Portugal
2008 - 2008
Concluded
 Successful Approaches to Effective Real-Time RT PCR and From Petunia to Mammals siRNA, a tool to study gene function (Curso de Especialização Tecnológica)
Universidade do Minho Instituto de Investigação em Ciências da Vida e Saúde, Portugal
2008 - 2008
Concluded
 Microarray: Theory and pratical. From RNA extraction to Functional data analysis (Curso de Especialização Tecnológica)
Universidade do Minho Instituto de Investigação em Ciências da Vida e Saúde, Portugal
2006/01 - 2007/07
Concluded
 Certification of Professional Training in Environmental Management in a Company (Curso Técnico Superior Profissional)
Winnerges, Portugal
2005/05/13
Concluded
 Ciências do Ambiente / Environmental Sciences (Mestrado)
Major in Qualidade Ambiental / Environmental Quality
Universidade do Minho Departamento de Biologia, Portugal
 Very Good
2004/11 - 2004/12
Concluded
 Certification of Professional Training "Sampling, Analysis and Significance of Environmental Aspects (Curso Técnico Superior Profissional)
Universidade Católica Portuguesa Escola Superior de Biotecnologia, Portugal
2001/12/11
Concluded
 Biologia Aplicada - Applied Biology (Licenciatura)
Universidade do Minho Departamento de Biologia, Portugal
 14
Affiliation

Science

Category
Host institution 		Employer
2023/07/03 - 2027/02 Auxiliary Researcher (Research) Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal
Universidade do Porto Faculdade de Engenharia, Portugal
2019/03/10 - 2023/06/30 Researcher (Research) Universidade do Porto Faculdade de Engenharia, Portugal
Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal
2015/01/01 - 2019/03 Visiting Researcher (Research) Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal
2013/05 - 2015/01 Postdoc (Research) Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal
2008/01/01 - 2012/02/28 Research Trainee (Research) Universidade do Minho Departamento de Engenharia Biológica, Portugal
2006/04/15 - 2006/10/31 Researcher (Research) Universidade do Minho Centro de Engenharia Biológica, Portugal

Positions / Appointments

Category
Host institution 		Employer
2014 - 2021/12/31 Co-founder and Secretary of the General Assembly of Biomode SA Biomode SA, Portugal

Others

Category
Host institution 		Employer
2020/01/01 - 2021/12/31 Responsible for coordination of BLS2 laboratory at LEPABE Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal
Universidade do Porto, Portugal
2015/01/20 - 2018/09/21 Chief Operations Officer Biomode SA, Portugal
2012/03/16 - 2013/11/15 Researcher Biomode SA, Portugal
2013 - 2013 Clinical study coordinator Helicobacter pylori /resistance to Clarithromycin. DL 98/79/EC (IVD); Pre-IDE of FDA Biomode SA, Portugal
2006/11/01 - 2007/12/01 Owner Parapharmacy Farma Vida Sã, Portugal
2005 - 2005 Sales Representative Daquimed, Portugal
Projects

Grant

Designation Funders
2006 - 2006 Surveillance and control of microbiological stability in drinking water distribution networks (SAFER)
EVK1-CT-2002-00108
Research Fellow

Contract

Designation Funders
2022/09 - Current ERA Chair for Creation of a group of Excellence on Engineered Biofilms (e.Biofilm) HORIZON-CSA, HORIZON-WIDERA-2022-TALENTS-01. (Project writing; researcher; project manager))
1
Researcher
Ongoing
2021/01/01 - 2025/12/31 ALICE - Associate Laboratory in Chemical Engineering
LA/P/0045/2020
10.54499/LA/P/0045/2020
Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal

Universidade do Porto Faculdade de Engenharia, Portugal

Universidade do Porto Centro de Estudos de Fenómenos de Transporte, Portugal

Universidade do Porto Laboratório de Processos de Separação e Reacção Laboratório de Catálise e Materiais, Portugal

Instituto Politécnico de Leiria, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2021/10/04 - 2025/10/05 COST Action CA20110, RNA communication across kingdoms: new mechanisms and strategies in pathogen control (exRNA-PATH) (Management Committee Member from Portugal / Working Group member (2/3/4))
CA20110
Management Committee Member from Portugal / Working Group member (2/3/4)
Ongoing
2020/01/01 - 2023/12/31 Laboratory for Process Engineering, Environment, Biotechnology and Energy
UIDP/00511/2020
10.54499/UIDP/00511/2020
Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal

Universidade do Porto Faculdade de Engenharia, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2020/01/01 - 2023/12/31 Laboratory for Process Engineering, Environment, Biotechnology and Energy
UIDB/00511/2020
10.54499/UIDB/00511/2020
Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal

Universidade do Porto Faculdade de Engenharia, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2021/01/01 - 2023/06/30 FLUDS - Development of fluorescence spectroscopy systems for microbial detection (NORTE-01-0247-FEDER-046970) (Responsible for the project writing; researcher; project manager)
C578918019-00129680
Researcher
SARSPEC, Portugal

Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal

Instituto Nacional de Investigação Agrária e Veterinária, Portugal
Concluded
2020 - 2021/12/31 NAM4Covid19: Nucleic acid mimic lateral flow assay (NAM-LFA) for the detection of Covid19
596771122
Researcher
Instituto Nacional de Investigação Agrária e Veterinária, Portugal

Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal

Instituto Nacional de Saúde Doutor Ricardo Jorge, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2018/07/16 - 2021/09/15 Color-ISH - Development of a new colorimetric method for the rapid detection of Legionella spp in water samples
POCI-01-0145-FEDER-029961
Principal investigator
Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal

Instituto Nacional de Investigação Agrária e Veterinária, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2018/07/26 - 2021/07/25 NAM4 Toxins - De novo selection of toxin-specific aptamers using nucleic acid mimics (PTDC/BAA-DIG/28659/2017)
PTDC/BAA-DIG/28659/2017
Researcher
Fundação para a Ciência e a Tecnologia
Concluded
2018 - 2021 µFISH - Integration of fluorescence in situ hybridization with microfluidics for the quasi real-time detection of food pathogens (PTDC/EQU-EQU/31011/2017)
POCI-01-0145-FEDER-0301011
Researcher
Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal

Universidade do Porto Centro de Estudos de Fenómenos de Transporte, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2017/07/01 - 2019/07/01 Coded-FISH - Development of a new RING FISH method with color code: an innovative tool for the detection of antibiotic resistant pathogens
POCI-01-0145-FEDER-016678
Researcher
Concluded
2018 - 2018 Diagnosing Pathogen Bacteria Earlier and More Accurately with Biomode’s Probe4@ Rapid Diagnostic Kits (SME Instrument)
REP-815522-1
Principal investigator
Biomode SA, Portugal

Universidade do Porto Faculdade de Engenharia, Portugal
European Commission
Concluded
2014/12/31 - 2018 Nanofluidics for ultrafast diagnosis of bacterial infections (NanoDiaBac)
ENMED/0001/2014
Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2015 - 2016 Epidemiology of H. pylori in Equador and its relation to gastric cancer
Collaboration Grants 2014-2015
Universidad San Francisco de Quito, Ecuador
Concluded
2015 - 2016 Biomode SA Business Internationalization
NORTE-02-0752-FEDER-002490
Other
Biomode SA, Portugal
 European Commission
Concluded
2012/02/01 - 2014 “DNA mimics” - Development and application of DNA mimics for the rapid identification of pathogens
info:eu-repo/grantAgreement/FCT/5646-ICCMS/82815/PT
 Fundação para a Ciência e a Tecnologia
Concluded
2012 - 2013 QREN Internationalization
NORTE-07-00201-FEDER-025889
Other
European Commission
Concluded
2010/04/01 - 2013 HELIWATER - Water and associated biofilms as a possible vector for Helicobacter pylori transmission among the human population
info:eu-repo/grantAgreement/FCT/5876-PPCDTI/108811/PT
Universidade do Minho Departamento de Engenharia Biológica, Portugal
Concluded
2010 - 2013 Peptide Nucleic acid as an emerging technology for the detection of H. pylori in food
NANO/NTec-SQA/0168/2007
Researcher
Universidade do Minho Departamento de Engenharia Biológica, Portugal
2012 - 2012 Intellectual Property
NORTE-07-00201-FEDER-025012
Other
European Commission
Concluded
2010/04/01 - 2012 HeliSysBio - Molecular Systems Biology Helicobacter pylori
info:eu-repo/grantAgreement/FCT/5876-PPCDTI/104235/PT

Other

Designation Funders
2024/09/26 - 2028/09/25 CA23152 - Building Consensus on Biofilm Regulatory Decision Making (RegulatoryToolBox)
CA23152
Researcher
Ongoing
2011 - 2012 Strategic project LA 23 - 2011-2012
LA 23-2011-2012
Researcher
Outputs

Publications

Book chapter
  1. Araújo, Daniela; Oliveira, Ricardo; Ana Maria Campos; Maria Margarida Barros; Andreia S. Azevedo; Ana Barbosa; Laura Cerqueira; et al. "Exploring the Spectrum of Biofilm Complexity". In Microbial Biofilm Dynamics.. Taylor & Francis Group, LLC, 2024.
    Accepted
  2. Joana Castro; Ricardo Oliveira; Ana Maria Campos; Maria Margarida Barros; Andreia S. Azevedo; Ana Barbosa; Laura Cerqueira; et al. "Laboratory Models for Biofilm Studies". In Microbial Biofilm Dynamics. 2024.
    Accepted
  3. Almeida, Carina; Azevedo, NF; Cerqueira, L.; Keevil, C.W.; Vieira, MJV. "RT-PCR and PNA-FISH as novel methods for the detection of specific microorganisms in heterotrophic biofilm communities". In Biofilms: Coming of Age,, 207-216. Manchester, United Kingdom: The Biofilm Club, 2007.
    Published
Conference abstract
  1. Azevedo, Nuno F.; Goeres, Darla M.; Cerqueira, Laura. "A Novel Multiplex mRNA PNA-FISH for Studying Gene Expression and Spatial Organization in Biofilms". Paper presented in EuroBioFilms 2024, Copenhagen, 2024.
    Published
  2. Barbosa, Ana; Darla Goeres; Azevedo, Nuno Filipe; Cerqueira, Laura. "PNA-FISH applications for bacterial detection and gene expression assessment in biofilms". Paper presented in SurfSAFE Conference - Biofilm detection and control in the food industry, Porto, 2023.
    Published
  3. Cerqueira, L.. "Lighting up Pathogen Detection: The Use of PNA-FISH for the Detection of Pathogens in Food and Water Samples". Paper presented in International Association for Food Protection: IAFP's 2022 European symposium, Munique, 2022.
  4. Rocha R; Sousa J.M.; Cerqueira, L.; Vieira M.J; Almeida C.; N. F. Azevedo. "Development and application of Peptide Nucleic Acid Fluorescence in situ Hybridization for the specific detection of Listeria monocytogenes". Paper presented in International Association for Food Protection: IAFP's 2017 European symposium, 2017.
  5. Cerqueira, Laura; Azevedo, Nuno Filipe; Faria, Nuno Torres; Guimarães, Nuno; Figueiredo, Ceu; Keevil, Charles William; Vieira, Maria João. "New Diagnostic Method for the Detection of Clarithromycin Resistance in H. pylori Strains Using Peptide Nucleic Acid Probes". Paper presented in Helicobacter, 2009.
    Published
  6. Cerqueira, Laura; Guimarães, N; Figueiredo, Céu; Keevil, C.W.. "Use of peptide nucleic acid probes as a new method for the detection of clarithromycin resistance in Helicobacter pylori strains". Paper presented in Clinical Microbiology and Infection, 2009.
  7. Machado, Idalina; Cunha, Rita; Cerqueira, Laura; Lopes, S. P; Azevedo, N.F.,; Pereira, Olívia. "Influence of ionic strength in P. aeruginosa early-biofilm development". Paper presented in EUROBIOFILMS 2009 - First European Congress on Microbial Biofilms,, 2009.
    Published
  8. Cerqueira, Laura; Grade, Nuno; Vingada, José. "Diet and Distribution of Otter in South Portugal, Ria Formosa Natural Park". Paper presented in European Otter Conference, 2004.
Conference paper
  1. Almeida, Carina; Cerqueira, L.; Azevedo, N. F.; Vieira, M. J.. "Helicobacter pylori : role of water on survival and transmission". 2012.
  2. Cerqueira, L.; Fernandes, R. M.; Ferreira, Rui M.; Carneiro, F.; Ribeiro, M. Dinis; Figueiredo, C.; Azevedo, N. F.; Keevil, C. W.; Vieira, M. J.. "Application of fluorescence in situ hybridisation using peptide nucleic acid probes in gastric samples for detection of Helicobacter pylori clarithromycin resistance". 2011.
  3. Almeida, Carina; Azevedo, N. F.; Cerqueira, L.; Bento, Joana C.; Vieira, M. J.; Keevil, C. W.. "Development of a rapid method for Proteus spp. detection in urine by peptide nucleic acid fluorescence in situ hybridisation (PNA)". 2011.
Conference poster
  1. Barbosa VB; Cerqueira, L.; João M Miranda; Nuno F Azevedo. "Deterministic lateral displacement for separation in microscale: particle and microbial cell analysis". Paper presented in 40th International Symposium on Microscale Separation and Bioanalysis (MSB 2024), 2024.
  2. Barbosa VB; Cerqueira, L.; Miranda JM; Azevedo NF. "Evaluation of separation in DLD microchannel". Paper presented in 5th Doctoral Congress in Engineering (DCE 2023), 2023.
  3. Violina Barbosa; Célia Rodrigues; Laura Cerqueira; João M. Miranda; Nuno F. Azevedo. "Candida tropicalis detection with fluorescence in situ hybridization (FISH)-based microfluidic platform". Paper presented in 19th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID). Helsínquia, Finlândia, 2022.
  4. Oliveira, Ricardo; Rocha, Rui; Sousa, Mário; Cerqueira, Laura; Azevedo, Nuno Filipe; Almeida, Carina. "Rapid identification of Campylobacter spp. using peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) in food samples". Paper presented in V AEICBAS Biomedical Congress - Abel Salazar Biomedical Sciences Institute (ICBAS), Porto, Portugal, 2017.
  5. Fontenete S; Azevedo, Andreia S; Rocha, R.; Santos RS; Almeida Carina; Cerqueira, Laura; Guimarães N; Doctor Nuno F Azevedo (¿Faculty of Engineering, University of Porto). "Application of nucleic acid mimics in fluorescence in situ hybridization for the visualization of naturally- occurring biofilms with minimal disruption". Paper presented in Biofilms 6, 2014.
  6. Cerqueira, Laura; Fernandes, Ricardo; Ferreira, Rui; Oleastro, Mónica; Carneiro, Fátima; Brandão, C; Pimentel-Nunes, Pedro; et al. "Probe4Pylori®: a new kit for the rapid detection of H. pylori and associated clarithromycin resistance in gastric biopsies". Paper presented in XXVIth International Workshop on Helicobacter and related bacteria in chronic digestive inflammation and gastric cancer., 2013.
  7. Cerqueira, Laura; Fernandes, Ricardo; Ferreira, Fátima Carneiro; Dinis-Ribeiro, M.; Ceu Figueiredo; Keevil, C.W.; Azevedo, NF; Vieira, MJV. "Application of fluorescence in situ hybridization using peptide nucleic acid probes in gastric samples for detection of Helicobacter pylori clarithromycin resistance". Paper presented in 21st European Congress of Clinical Microbiology and Infectious Diseases 27h Int. Congress of Chemotherapy Group, 2011.
  8. Cerqueira, Laura; Azevedo, NF; Faria, N; Guimarães, N; Figueiredo, C; Keevil, C.W.; Vieira, MJ. "New diagnostic method for the detection of clarithromycin resistance in Helicobacter pylori strains, using peptide nucleic acid probes". Paper presented in XXIInd international Workshop of the European Helicobacter Study Group. Porto, Portugal, 2009.
  9. Cerqueira, Laura; Azevedo, NF; Guimarães, N; Figueiredo, C; Keevil, C.W.; Vieira, MJ. "Use of peptide nucleic acid probes as a new method for the detection of clarithromycin resistance in Helicobacter pylori strains". Paper presented in 19th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID). Helsínquia, Finlândia, 2009.
  10. Cerqueira, Laura; alves, josé; Vingada, José. "Poster". Paper presented in XXIX International Congress of Limnology (SIL), LAHTI, 2004.
Journal article
  1. Sousa, José Mário; Barbosa, Ana; Araújo, Daniela; Castro, Joana; Azevedo, Nuno Filipe; Cerqueira, Laura; Almeida, Carina. Corresponding author: Cerqueira, Laura. "Evaluation of Simultaneous Growth of Escherichia coli O157:H7, Salmonella spp., and Listeria monocytogenes in Ground Beef Samples in Different Growth Media". Foods 13 13 (2024): 2095. http://dx.doi.org/10.3390/foods13132095.
    10.3390/foods13132095
  2. Almeida, Carina; Cerqueira, Laura. Corresponding author: Cerqueira, Laura. "Editorial: Molecular diagnostic methods for bacteria and fungi detection". Frontiers in Cellular and Infection Microbiology 14 (2024): http://dx.doi.org/10.3389/fcimb.2024.1430630.
    Published • 10.3389/fcimb.2024.1430630
  3. Sousa, Mário; Rocha, Rui; Araújo, Daniela; Castro, Joana; Barbosa, Ana; Azevedo, Nuno F.; Cerqueira, Laura; Almeida, Carina. Corresponding author: Almeida, Carina. "A New Peptide Nucleic Acid Fluorescence In Situ Hybridization Probe for the Specific Detection of Salmonella Species in Food Matrices". Foodborne Pathogens and Disease 21 5 (2024): 298-305. http://dx.doi.org/10.1089/fpd.2023.0127.
    Published • 10.1089/fpd.2023.0127
  4. Oliveira, Ricardo; Barbosa, Ana; Sousa, Mário; Azevedo, Nuno Filipe; Cerqueira, Laura; Almeida, Carina. Corresponding author: Cerqueira, Laura. "Using peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) to detect Campylobacter spp. in food samples". LWT 198 (2024): 115922. http://dx.doi.org/10.1016/j.lwt.2024.115922.
    Published • 10.1016/j.lwt.2024.115922
  5. Barbosa, Ana; Azevedo, Nuno F.; Goeres, Darla M.; Cerqueira, Laura. Corresponding author: Cerqueira, Laura. "Ecology of Legionella pneumophila biofilms: The link between transcriptional activity and the biphasic cycle". Biofilm (2024): 100196. http://dx.doi.org/10.1016/j.bioflm.2024.100196.
    10.1016/j.bioflm.2024.100196
  6. Barbosa A; Miranda S; Azevedo N; Cerqueira L; Azevedo A. "Imaging biofilms using fluorescence in situ hybridization: seeing is believing". Front. Cell. Infect. Microbiol. Sec. Biofilms Volume 13 - 2023 | doi: 10.3389/fcimb.2023.1195803 (2023):
    Accepted
  7. Cerqueira, L.; Miranda, J.M.. Corresponding author: Cerqueira, L.. "Editorial for the Special Issue on Microfluidics and Lab-on-a-Chip Applications for Biosensing". Micromachines MDPI (2022): https://www.mdpi.com/2072-666X/13/12/2060/htm.
    Published
  8. Violina Barbosa; Célia Rodrigues; Cerqueira, L.; João Mário Miranda; Azevedo, Nuno Filipe. Corresponding author: João Mário Miranda. "Microfluidics combined with fluorescence in situ hybridization (FISH) for Candida tropicalis detection (corresponding author Laura Cerqueira / João Miranda)". Frontiers in Microbiology (2022): https://doi.org/10.3389/fbioe.2022.987669.
    Published
  9. Nácher-Vázquez, Montserrat; Barbosa, Ana; Inês Armelim; Andreia Sofia Azevedo; Gonçalo Nieto Almeida; Cristina Pizarro ; Nuno Filipe Azevedo; Carina Almeida; Laura Cerqueira. Corresponding author: Laura Cerqueira. "Development of a Novel Peptide Nucleic Acid Probe for the detection of Legionella spp. in Water Samples". MDPI Microorganisms (2022): DOI: 10.3390/microorganisms10071409.
    Published
  10. Nacher-Vasquez, Montserrat ; Santos, Bruno ; Azevedo, Nuno F.; Cerqueira, Laura. Corresponding author: Cerqueira, Laura. "The role of Nucleic Acid Mimics (NAMs) on FISH-based techniques and applications for microbial detection". Microbiological Research (2022): https://doi.org/10.1016/j.micres.2022.127086.
    Published
  11. Joana Filipa Lima; Paulo Maia; Beatriz T. Magalhães; Cerqueira, Laura; Nuno F. Azevedo. "A comprehensive model for the diffusion and hybridization processes of nucleic acid probes in fluorescence in situ hybridization (IF (2021): 4.002)". Biotechnology and Bioengineering (2020): DOI: 10.1002/bit.27462.
    Published
  12. Cerqueira, Laura; Sara Moura; Almeida, Carina; Maria João Vieira; Nuno Filipe Azevedo. Corresponding author: Cerqueira, Laura. "Establishment of a New PNA-FISH Method for Aspergillus fumigatus Identification: First insights for future use in Pulmonary Samples (IF: 4.152)". Microorganisms (2020): DOI: 10.3390/microorganisms8121950.
    Open access • Published
  13. Rocha, Rui; Sousa, José M.; Cerqueira, Laura; Vieira, Maria J.; Almeida, Carina; Azevedo, Nuno F.. "Development and application of Peptide Nucleic Acid Fluorescence in situ Hybridization for the specific detection of Listeria monocytogenes (IF: 4.155)". Food Microbiology 80 (2019): 1-8. http://dx.doi.org/10.1016/j.fm.2018.12.009.
    Published • 10.1016/j.fm.2018.12.009
  14. Sousa, J. Mário; Rocha, Rui; Cerqueira, Laura; Almeida, Carina; Azevedo, Nuno F.; Bastin, Benjamin; Bird, Patrick; et al. "Validation of Biomode S.A. Probe4CronobacterTM for the Identification of Cronobacter spp.". Journal of AOAC International 102 3 (2019): 855-864. http://dx.doi.org/10.5740/jaoacint.18-0328.
    Published • 10.5740/jaoacint.18-0328
  15. Lima, Joana Filipa; Carvalho, Joana; Pinto-Ribeiro, Inês; Almeida, Carina; Wengel, Jesper; Cerqueira, Laura; Figueiredo, Céu; Oliveira, Carla; Azevedo, Nuno Filipe. "Targeting miR-9 in gastric cancer cells using locked nucleic acid oligonucleotides (IF (2019): 2.57)". BMC Molecular Biology 19 1 (2018): http://dx.doi.org/10.1186/s12867-018-0107-6.
    Accepted • 10.1186/s12867-018-0107-6
  16. Moura, S.; Cerqueira, L.; Almeida, A.. "Invasive pulmonary aspergillosis: current diagnostic methodologies and a new molecular approach (IF(2019):2.837)". European Journal of Clinical Microbiology & Infectious Diseases 37 8 (2018): 1393-1403. http://dx.doi.org/10.1007/s10096-018-3251-5.
    Published • 10.1007/s10096-018-3251-5
  17. Joana Filipa Lima; Laura Cerqueira; Ceu Figueiredo; Carla Oliveira; Nuno Filipe Azevedo. "Anti-miRNA oligonucleotides: A comprehensive guide for design (5.414 (2019) 5 year IF)". RNA Biology 15 3 (2018): 338-352. https://doi.org/10.1080/15476286.2018.1445959.
    Accepted • 10.1080/15476286.2018.1445959
  18. Müller, Vilhelm; Sousa, José M.; Ceylan Koydemir, Hatice; Veli, Muhammed; Tseng, Derek; Cerqueira, Laura; Ozcan, Aydogan; Azevedo, Nuno F.; Westerlund, Fredrik. "Identification of pathogenic bacteria in complex samples using a smartphone based fluorescence microscope (IF(2019):3.119)". RSC Advances 8 64 (2018): 36493-36502. http://dx.doi.org/10.1039/c8ra06473c.
    Published • 10.1039/c8ra06473c
  19. Ferreira, André M.; Cruz-Moreira, Daniela; Cerqueira, Laura; Miranda, João M.; Azevedo, Nuno F.. "Yeasts identification in microfluidic devices using peptide nucleic acid fluorescence in situ hybridization (PNA-FISH)". Biomedical Microdevices 19 1 (2017): http://dx.doi.org/10.1007/s10544-017-0150-y.
    10.1007/s10544-017-0150-y
  20. Almeida, C.; Constante, D.; Ferreira, A.; Cerqueira, L.; Vieira, M.J.; Azevedo, N.F.. "A new colorimetric peptide nucleic acid-based assay for the specific detection of bacteria". Future Microbiology 9 10 (2014): 1131-1142. http://www.scopus.com/inward/record.url?eid=2-s2.0-84911493243&partnerID=MN8TOARS.
    10.2217/FMB.14.68
  21. Almeida, C.; Sousa, J.M.; Rocha, R.; Cerqueira, L.; Fanning, S.; Azevedo, N.F.; Vieira, M.J.. "Detection of Escherichia coli O157 by peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) and comparison to a standard culture method". Applied and Environmental Microbiology 79 20 (2013): 6293-6300. http://www.scopus.com/inward/record.url?eid=2-s2.0-84885043580&partnerID=MN8TOARS.
    10.1128/AEM.01009-13
  22. Almeida, C.; Cerqueira, L.; Azevedo, N.F.; Vieira, M.J.. "Detection of Salmonella enterica serovar Enteritidis using real time PCR, immunocapture assay, PNA FISH and standard culture methods in different types of food samples (Impact Factor: 4.187; 2019)". International Journal of Food Microbiology 161 1 (2013): 16-22. http://www.scopus.com/inward/record.url?eid=2-s2.0-84871998766&partnerID=MN8TOARS.
    10.1016/j.ijfoodmicro.2012.11.014
  23. Cerqueira, L.; Fernandes, R.M.; Ferreira, R.M.; Oleastro, M.; Carneiro, F.; Brandão, C.; Pimentel-Nunes, P.; et al. "Validation of a fluorescence in situ hybridization method using peptide nucleic acid probes for detection of Helicobacter pylori clarithromycin resistance in gastric biopsy specimens (IF (2019): 4.590)". Journal of Clinical Microbiology 51 6 (2013): 1887-1893. http://www.scopus.com/inward/record.url?eid=2-s2.0-84878492257&partnerID=MN8TOARS.
    10.1128/JCM.00302-13
  24. Cerqueira, L.; Oliveira, J.A.; Nicolau, A.; Azevedo, N.F.; Vieira, M.J.. "Biofilm formation with mixed cultures of Pseudomonas aeruginosa/Escherichia coli on silicone using artificial urine to mimic urinary catheters". Biofouling 29 7 (2013): 829-840. http://www.scopus.com/inward/record.url?eid=2-s2.0-84880401428&partnerID=MN8TOARS.
    10.1080/08927014.2013.807913
  25. Azevedo, N.F.; Bragança, S.M.; Simes, L.C.; Cerqueira, L.; Almeida, C.; Keevil, C.W.; Vieira, M.J.. "Proposal for a method to estimate nutrient shock effects in bacteria". BMC Research Notes 5 (2012): http://www.scopus.com/inward/record.url?eid=2-s2.0-84864569924&partnerID=MN8TOARS.
    10.1186/1756-0500-5-422
  26. Cerqueira, L.; Fernandes, R.M.; Ferreira, R.M.; Carneiro, F.; Dinis-Ribeiro, M.; Figueiredo, C.; Keevil, C.W.; Azevedo, N.F.; Vieira, M.J.. "PNA-FISH as a new diagnostic method for the determination of clarithromycin resistance of Helicobacter pylori". BMC Microbiology 11 (2011): http://www.scopus.com/inward/record.url?eid=2-s2.0-79955854378&partnerID=MN8TOARS.
    10.1186/1471-2180-11-101
  27. Azevedo, N.F.; Jardim, T.; Almeida, C.; Cerqueira, L.; Almeida, A.J.; Rodrigues, F.; Keevil, C.W.; Vieira, M.J.. "Application of flow cytometry for the identification of Staphylococcus epidermidis by peptide nucleic acid fluorescence in situ hybridization (PNA FISH) in blood samples". Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology 100 3 (2011): 463-470. http://www.scopus.com/inward/record.url?eid=2-s2.0-80054101666&partnerID=MN8TOARS.
    10.1007/s10482-011-9595-9
  28. Azevedo, N.F.; Almeida, C.; Fernandes, I.; Cerqueira, L.; Dias, S.; Keevil, C.W.; Vieira, M.J.. "Survival of gastric and enterohepatic Helicobacter spp. in water: Implications for transmission". Applied and Environmental Microbiology 74 6 (2008): 1805-1811. http://www.scopus.com/inward/record.url?eid=2-s2.0-40849087921&partnerID=MN8TOARS.
    10.1128/AEM.02241-07
  29. Cerqueira, L.; Azevedo, N.F.; Almeida, C.; Jardim, T.; Keevil, C.W.; Vieira, M.J.. "DNA mimics for the rapid identification of microorganisms by fluorescence in situ hybridization (FISH) (5-year Impact Factor: 4.653)". International Journal of Molecular Sciences 9 10 (2008): 1944-1960. http://www.scopus.com/inward/record.url?eid=2-s2.0-54349113792&partnerID=MN8TOARS.
    10.3390/ijms9101944
  30. Azevedo, N.F.; Almeida, C.; Cerqueira, L.; Dias, S.; Keevil, C.W.; Vieira, M.J.. "Coccoid form of Helicobacter pylori as a morphological manifestation of cell adaptation to the environment". Applied and Environmental Microbiology 73 10 (2007): 3423-3427. http://www.scopus.com/inward/record.url?eid=2-s2.0-34249660577&partnerID=MN8TOARS.
    10.1128/AEM.00047-07
Thesis / Dissertation
  1. Cerqueira, Laura. "Development and Evaluation of peptide nucleic acid (PNA) probes for the rapid detection of clinically relevant microorganisms". PhD, 2012.

Intellectual property

Patent
  1. Cerqueira, L.. 2022. "Laura Cerqueira; Montse-Vásquez; Carina Almeida; Nuno Azevedo. SONDA DE ÁCIDO PÉPTIDO NUCLEICO (PNA), ESTOJO E MÉTODO PARA DETECTAR E/OU QUANTIFICAR LEGIONELLA SPP. E RESPECTIVAS APLICAÇÕES".
  2. Cerqueira, Laura; Almeida, Carina; Azevedo, NF; Vieira, MJ. 2014. "Peptide Nucleic Acid probe (PNA), Kit and method for detection of Aspergillus fumigatus and applications thereof".
  3. Almeida, C.; De Sousa, José Mário; Rocha, Rui; Cerqueira, Laura; Vieira, MJ; Azevedo, NF. 2014. "Peptide Nucleic Acid (PNA) Probe, Kit and Method for detecting and/or quantifying Escherichia coli O157:H7".
  4. Almeida, C.; Cerqueira, Laura; Vieira, MJ; Azevedo, NF. 2014. "A blocker probe to avoid non-specific detection of Listeria monocytogenes, method and applications thereof".
  5. Azevedo, NF; Cerqueira, Laura; Faria, Ricardo. 2009. "Peptide nucleic acid probes, kit and method for detecting helicobacter pylori and/or clarithromycin resistance profile and applications".

Other

Legal proceeding
  1. 2016. Technical File for Probe4Pylori CE Mark (D.L. 98/79/EC (IVD) ) and PRE-IDE FDA.
Other output
  1. Probe4Salmonella Test Kit Certification by AOAC RI. This method has been evaluated in the AOAC® Performance Tested MethodsSM Program, and found to perform as stated by the manufacturer contingent to the comments contained in the manuscript. This certificate means that an AOAC® Certification Mark License Agreement has been executed which authorizes the manufacturer to display the AOAC Performance Tested SM certification mark.. 2018. De Sousa, José Mário; Rocha, Rui; Cerqueira, Laura; Almeida, Carina; Azevedo, Nuno Filipe.
  2. Probe4Monocytogenes Test Kit Certification by AOAC RI. This method has been evaluated in the AOAC® Performance Tested MethodsSM Program, and found to perform as stated by the manufacturer contingent to the comments contained in the manuscript. This certificate means that an AOAC® Certification Mark License Agreement has been executed which authorizes the manufacturer to display the AOAC Performance Tested SM certification mark. 2017. Rocha, Rui; De Sousa, José Mário; Cerqueira, Laura; Almeida, Carina; Azevedo, Nuno Filipe.
  3. Probe4Cronobacter Test Kit Certification by AOAC RI. first product in the world certified by AOAC for Cronocbacter Evaluation under the AOAC® PTM Program, and found to perform as stated by the manufacturer contingent to the comments contained in the manuscript. This certificate means that an AOAC® Certification Mark License Agreement has been executed which authorizes the manufacturer to display the AOAC Performance Tested SM certification mark;. 2017. Rocha, Rui; De Sousa, José Mário; Cerqueira, Laura; Almeida, Carina; Azevedo, Nuno Filipe.
  4. Biomode quality certification system by ISO 9001. IQNet and AENOR certified Biomode for the following field of activities: Investigation and development for Design and production of peptide nucleic acid probes kits for rapid and direct identification of pathogenic microorganisms by fluorescence microscopy in food processing and clinical areas (in vitro research and diagnosis). 2016. Cerqueira, Laura; Lima, Joana.
  5. Biomode quality certification system by ISO 13485 - Medical Devices production. IQNet and AENOR certified Biomode SA for the following field of activities: Investigation and development for design and production of peptide nucleic acid probes kits for rapid and drect identification of pathogenic microorganisms by fluorescence microscopy for in vitro diagnosis.. 2016. Cerqueira, Laura; Lima, Joana.
Spin-off company
  1. 2010. Biomode SA. www.biomode-sa.com.
  2. 2007. Farma Vida Sã. Health care products selling.
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2023 PNA-FISH applications for Legionella detection and localization in biofilms
5th Doctoral Congress in Engineering. Faculty of Engineering of the University of Porto (Porto, Portugal)
2023 PNA-FISH as a tool for exRNA detection in biofilms
COST Action CA20110 meeting, Centre Culturel Irlandais (Paris, France)
2022/07/13 Application of PNA-FISH based-methods for bacterial detection and localization in biofilms (Invited Speaker / Young Investigator Award) annual Montana Biofilm Science & Technology Meeting
Center for Biofilm Engineering - Montana State University (Bozeman, United States)
2022/06 NAM-FISH based methods for bacterial detection COST Action meeting
Univ. Turin (Turin, Italy)
2022/05/05 Lighting up Pathogen Detection: The Use of PNA-FISH for the Detection of Pathogens in Food and Water Samples International Association for Food Protection (IAFP) 2022 - European Symposium
(Munique, Germany)
2021/11 Barbosa VB; Rodrigues CF; Cerqueira L; Miranda J; Azevedo NF. Microfluidics combined with fluorescence in situ hybridization (FISH) for Candida tropicalis detection EUROPT(R)ODE XV, 29
(warsaw, Poland)
2021/05/04 Taking FISH from Lab to Market (invited speaker) SS2 "FISHing Bacteria"
FEUP_LEPABE (DELNAM) (Porto, Portugal)
2021 Violina Barbosa; Célia Rodrigues; Laura Cerqueira; João Miranda; Nuno Azevedo: COmbination of microfluidics and fluorescence in situ hybridization to detect Candida tropicalis in urine samples. Flash presentation - Session B. British yeast group 2021 - The future of yeast research
Microbiology Society
2020/07 Biomode VIbrant - Workshop 6 - Diagnostic development (online Workshop)
CeNTI, V. N. Famalicão, Portugal (Portugal)
2017/07/04 Biomode Company presentation to MIT MIT presentations
INL (Braga, Portugal)
2017/03/17 DIVs - Marcação CE Biomode Investbraga - Medical Devices
StartupBraga (Braga, Portugal)
2017/02/23 Biomode presentation to Bayer InvestBraga startups presentation to Bayer
INL (Portugal)
2016/10/27 From Idea to Business FNAC Talks
COTEC (Braga, Portugal)
2015/04/07 Biomode Presentation: Biomode and the experience of a technology-based entrepreneur War Stories - COHITEC 2015 Program Seminar
COTEC (Porto, Portugal)
2015/03/17 Biomode Presentation - Portuguese reality Programa de Gestão da Inovação e Empreendedorismo da Universidade de Brown
COTEC (Porto, Portugal)
2014/09/12 Moreira, D., Cerqueira, L., Miranda, J., Azevedo, N. “Yeasts identification in microfluidic devices through peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) “. Yeasts identif ChemPor 2014 - 12th International Chemical and Biological Engineering Conference, FEUP, University of Porto
FEUP (Porto, Portugal)
2014/07/17 Biomode presentation Global Entrepreneurship Program Delegation to Portugal
COTEC (Porto, Portugal)
2013/09/16 Biomode presentation to Mister President Sir Cavaco Silva
Spinpark/Tecminho (Caldas das Taipas, Portugal)
2013/09/11 Biomode presentation to Caixa Capital - Investment
CaixaCapital (Lisboa, Portugal)
2013/07/19 Taking FISH from lab to market FISH: Fundamentals and Applications
LEPABE (Porto, Portugal)
2013/07/09 Biomode update Portugal Ventures
Portugal Ventures (Lisboa, Portugal)
2013/03/22 Biomode presentation to Biomérieux
(Caldas das Taipas, Portugal)
2012/11/09 Medical Devices Ciência e Engenharia - Dispositivos médicos
Universidade do Minho (Escola de ciências/Escola de Engenharia) (Guimarães, Portugal)
2008/09/20 Cerqueira, L., Azevedo, N.F., Guimarães, N., Figueiredo, C., Keevil, C.W., and Vieira, M.J. Development of peptide nucleic acid probes for the detection of different point mutations conferring clarithromycin resistance in Helicobacter pylori 23S rRNA XXIst international Workshop of the European Helicobacter Study Group. Riga, Latvia
European Helicobacter Study Group (Riga, Latvia)
2008/03/14 L. Cerqueira, N.F. Azevedo and M.J.Vieira. "Development of peptide nucleic acid probes for the detection of different point mutations conferring clarithromycin resistance in Helicobacter pylori 23S rRNA.", Reunião Luso-Francesa de Helicobacter pylori
Instituto Nacional de Saúde Dr. Ricardo Jorge, Lisbon, Portugal. (Lisboa, Portugal)

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2023/10 - Current Use of FISH and targeted transcriptomic imaging along with matrix stains to map an industrial multispecies biofilm that includes the pathogen Legionella
Co-supervisor of Sofia Cruz
 Phd (PhD)
Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal

Center for Biofilm Engineering - Montana State University, United States
2022/09/01 - 2026/08/31 Development of a multiplex mRNA PNA-FISH method to assess Legionella pneumophila morphological state within multispecies biofilms in water systems
Supervisor of Ana Barbosa
 PhD (PhD)
Universidade do Porto Faculdade de Engenharia, Portugal

Associated Laboratory in Chemical Engineering (ALiCE), Portugal

Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal

Center for Biofilm Engineering - Montana State University, United States
2022/09/01 - 2025/08/31 Integration of microfluidics and fluorescence in situ hybridization (FISH) for rapid identification of microorganisms in food samples
Co-supervisor of Violina Barbosa
 PhD (PhD)
Universidade do Porto Faculdade de Engenharia, Portugal

Associated Laboratory in Chemical Engineering (ALiCE), Portugal

Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal
2024/02 - 2024/05 Development of a Spatial Transcriptomics approach to study L. pneumophila biofilm ecology
Supervisor
 Licenciatura em Bioengenharia (Bioengenharia Molecular) (Degree)
Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal
2023/10 - 2024/02 Métodos de análise para biofilmes de Escherichia coli
Supervisor
 Licenciatura em Engenharia Química (Degree)
Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal
2023/02 - 2023/04 FLUDS - Development of fluorescence spectroscopy systems for microbial detection (NORTE-01-0247-FEDER-046970)
Co-supervisor of Ricardo Oliveira
Universidade do Porto Faculdade de Engenharia, Portugal
2022 - 2023 FLUDS - Development of fluorescence spectroscopy systems for microbial detection (NORTE-01-0247-FEDER-046970) NAM probes diffusion on standardized biofilms
Co-supervisor of Igor Resende
Universidade do Porto Faculdade de Engenharia, Portugal
2022/09/12 - 2022/10/05 Short Term Scientific Mission on the exRNA-PATH COST Action CA20110, “RNA communication across kingdoms: new mechanisms and strategies in pathogen control”.
Co-supervisor of Enkeleda Berberi
Universidade do Porto Faculdade de Engenharia, Portugal
2021/10/01 - 2022/09/01 Desenvolvimento de sistemas baseados em espectroscopia de fluorescência para deteção microbiana
Co-supervisor of Ana Teresa Barbosa
Universidade do Porto Faculdade de Engenharia, Portugal
2022/02/28 - 2022/05/31 Erasmus Programme Health and Environment in Development of a colorimetric method for the detection of Legionella spp.
Supervisor of Jorge Llopis García
Universidade do Porto Faculdade de Engenharia, Portugal
2022/02/28 - 2022/05/31 Erasmus Programme Health and Environment in Optimization of salmonella PNA-FISH probe conditions
Co-supervisor of Nieves Corbalán López
Universidade do Porto Faculdade de Engenharia, Portugal
2020/11 - 2021/02/01 IMPROVING A PATHOGEN DETECTION METHOD BASED ON NUCLEIC ACID MIMICS-IN SITU HYBRIDIZATION (NAM-ISH)
Supervisor of Joaquim de Almeida
 Mestrado Integrado Bioengenharia - Engenharia Biológica (SPI) (Master)
2019 - 2021 Legionella spp. PNA-FISH detection in water samples
Supervisor of Montserrat Nacher-Vasquez
Universidade do Porto Faculdade de Engenharia, Portugal
2020/02/10 - 2020/07/17 Project POCI-01-0145-FEDER-029961 - COLOR-ISH – Improvement of a colorimetric in situ hybridization method (COLOR-ISH) for the rapid detection of pathogenic bacteria
Supervisor of Inês Almerim
 Mestrado Integrado em Engenharia Química (Master)
Universidade do Porto Faculdade de Engenharia, Portugal
2020/02/10 - 2020/07 Project POCI-01-0145-FEDER-029961 - Improvement of a colorimetric in situ hybridization method (COLOR-ISH) by the screening of alternative options for the enzyme-NAM conjugate
Co-supervisor of Cedric Bouba
Universiteit Gent, Belgium
2018 - 2018 Development of NAM-FISH methods for the detection of microorganisms in clinical and food samples
Supervisor of Ana Catarina Sousa
Universidade de Aveiro, Portugal

Biomode SA, Portugal
2017 - 2017 Product of Biomode based in the PNA-FISH technology for the detection of Aspergillus fumigatus in the clinical area. From this master thesis it was published a review (DOI https://doi.org/10.1007/s10096-018-3251-5)
Supervisor of Sara Moura
Universidade de Aveiro, Portugal

Biomode SA, Portugal
2014 - 2017 The use of antisense DNA mimics for suppressing microRNAs involved in gastric cancer (PhD)
Co-supervisor of Joana Lima
 Sandwich PhD (PhD)
Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal

Universidade do Porto Instituto de Patologia e Imunologia Molecular, Portugal

Biomode SA, Portugal
2013 - 2013/10 Interference studies for a product based on PNA-FISH
Co-supervisor of Teresa Maria Conde Lopes
 Mestrado Integrado em Engenharia Biológica (Master)
Universidade do Minho Departamento de Engenharia Biológica, Portugal
2012 - 2013 Certification processes in food biotechnology area
Co-supervisor of Simão Freitas Félix
Universidade de Aveiro, Portugal

Biomode SA, Portugal
2012 - 2013 Application of PNA-FISH based method for the specific identification of Aspergillus fumigatus in clinical samples
Co-supervisor of Joana Santos
Universidade do Porto Faculdade de Engenharia, Portugal

Biomode SA, Portugal
2011 - 2012 Development of a PNA-FISH method for the detection of L. monocytogenes
Co-supervisor of Ana Isabel Rocha Fontão
Universidade do Minho, Portugal

Biomode SA, Portugal
2011 - 2012 Certification of processes and products in the biotechnology area
Co-supervisor of Vasco Rafael Fernandes dos Santos
Universidade do Minho, Portugal

Biomode SA, Portugal

Event organisation

Event name
Type of event (Role) 		Institution / Organization
2023/03/03 - 2023/03/03 e.Biofilm project Kick-off meeting organization, 3rd March 2023 at Department of Chemical Engineering FEUP, University of Porto. (2023/03/03 - 2023/03/03) Universidade do Porto Faculdade de Engenharia, Portugal
2021/01/01 - 2021/05/06 The 2nd DelNAM Summer School. The summer school will include theoretical sessions and hands-on practical sessions on the FISH technique and its applications. The practical sessions will include the design of oligonucleotides to target bacteria, standard FISH on planktonic and mixed biofilms, as well as variants including the application of liposomes, microfluidics and spectral image analysis. (2021/05/04 - 2021/05/06)
Workshop (Member of the Organising Committee)
 Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal
2013 - 2013 The workshop focused on the theoretical part of FISH and its applications with oral presentations and poster sessions and in a practical course, focused on different FISH techniques applied to the detection of bacteria. This one-day course allowed participants to have a hands-on experience on the diverse steps of two well-known hybridization techniques: PNA-FISH (peptide nucleic acid FISH) and CARD-FISH (catalyzed reporter deposition FISH). (2013/07/18 - 2013/07/20)
Workshop (Member of the Organising Committee)
 Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal
2011 - 2011 Third International Practical Course on Biofilm Sciences: Biofilms: from basic to emergent (2011/05/09 - 2011/05/13)
Workshop (Member of the Organising Committee)
 Universidade do Minho Departamento de Engenharia Biológica, Portugal
2009 - 2009 Second International Practical Course on Biofilm Sciences (2009/02/16 - 2009/02/21)
Workshop (Member of the Organising Committee)
 Universidade do Minho Departamento de Engenharia Biológica, Portugal
2006 - 2006 First International Practical Course on Biofilm sciences (2006/09/11 - 2006/09/16)
Workshop (Member of the Organising Committee)
 Universidade do Minho Departamento de Engenharia Biológica, Portugal
2003 - 2003 XXVIth International Congress of the International Union of Game Biologists (IUGB): Integrating Wildlife with People; Perdix Xth International Symposium (2003/09 - 2003/09)
Congress (Member of the Organising Committee)
 Universidade do Minho Departamento de Biologia, Portugal
2002/09 - 2003 VIth European Roe Deer Meeting; Caldas do Gerês (2003/04 - 2003/04)
Conference (Member of the Organising Committee)
 Universidade do Minho Departamento de Biologia, Portugal
2000 - 2000 II Jornadas de Biologia Aplicada (2000/11 - 2000/11)
Symposium
 Universidade do Minho Departamento de Biologia, Portugal

Event participation

Activity description
Type of event 		Event name
Institution / Organization
2021/09/20 - 2021/09/20 Webinar "Legionella testing: Latest approaches and new perspectives" Explore different approaches to Legionella testing Understand the key requirements of the new European directive on the quality of water intended for human consumption Gain advice from a multiutility laboratory on the analysis and management of Legionella in drinking water
Seminar
SelectScience, United Kingdom
2021/09/06 - 2021/09/06 Participation at the COST Academy online "Leadership workshop"
Workshop
 Leadership Workshop
2021/07/07 - 2021/07/07 CEMBO. EU Workshop on "Excellence in project Management"
CEMBO.EU, Netherlands
2020/07/13 - 2020/07/15 Attended the EMBL Conference: Microfluidics: Designing the Next Wave of Biological Inquiry
Conference
 EMBL Conference: Microfluidics: Designing the Next Wave of Biological Inquiry
European Molecular Biology Laboratory, Germany
2020/03/10 - 2020/03/10 From Farm to Fork - The Food Safety Journey. Exploring the various pain points of potential contamination in the processes involved, and regulations overseeing production
Other
 From Farm to Fork - The Food Safety Journey
PALL Laboratory, United States
2019/05/22 - 2019/05/22 Jornada Te´cnica sobre "Redes de a´gua pota´vel em edifi´cios hospitalares - Descontaminac¸a~o e controle da corrosa~o''FEUP, 22ndMay 2019
Symposium
 Jornada Te´cnica sobre "Redes de a´gua pota´vel em edifi´cios hospitalares - Descontaminac¸a~o e controle da corrosa~o'
Universidade do Porto Faculdade de Engenharia, Portugal
2018/06/12 - 2018/06/12 ACHEMA World Forum and Leading Show for the Process Industries - Meetings with Stakeholders
2018/04/10 - 2018/04/13 Analytica Exhibition 2018 - meetings with stakeholders
Exhibition
2017/06/05 - 2017/06/06 TECNET - Business Camp to talk about entrepreneurship and technology development
2016/07/31 - 2016/08/03 International Association for Food Protection Annual Meeting. The IAFP Annual Meeting was attended by more than 3,300 of the top industry, academic and governmental food safety professionals from six continents. IAFP 2016 was held at the America's Center in St. Louis, Missouri. (Stand)
2016/05/27 - 2016/05/29 Digestive Disease Along the Douro Valley (Hotel Douro Palace) (Stand)
2008/06 - 2008/06 3rd Technology Open Day – Health & Food Technologies, Braga
2006/04 - 2006/04 XV Porto cancer Meeting Helicobacter pylori: From infection to gastric cancer IPATIMUP, Porto
2006/04 - 2006/04 Seventh Progress Meeting, SAFER (European Programme), Drinking Water. University of Minho, Braga
2005/12 - 2005/12 VII jornadas da SECEM (Sociedade espanhola para a conservação e estudo dos mamíferos) Valência, Spain
2003/11 - 2003/11 IV Congresso de Ornitologia e II Jornadas Ibéricas de Ornitologia Aveiro, Portugal apresentação em poster como co-autora
2003/09 - 2003/09 XXVIth International Congress of the International Union of Game Biologists – IUGB, Integrating Wildlife with People” and Perdix Xth International Symposium, Braga
2003/07 - 2003/07 The return of the otter in Europe – Where and How?; The International Otter Survival Fund, Scotland
2002/09 - 2002/09 XXVth Congress of the International Union of Game Biologists – IUGB and Perdix IX International Symposium, Cyprus
2002/09 - 2002/09 2º Congresso Nacional de Conservação da Natureza. ICN,Lisboa
2000/11 - 2000/11 II Jornadas de Biologia Aplicada Universidade do Minho; Braga
1999/12 - 1999/12 I Jornadas de Biologia Aplicada University of Minho; Braga, Portugal
1999/03 - 1999/03 IV Encontro Nacional de Estudantes de Biologia (ENEB) Instituto Profissional do Terço Porto, Portugal

Jury of academic degree

Topic
Role 		Candidate name (Type of degree)
Institution / Organization
2023/12/04 A new endolysin to combat Helicobacter pylori infections and gastric cancer prevention
(Thesis) Main arguer
 Maria Inês Pinto Rocha (Master)
Universidade do Minho Departamento de Engenharia Biológica, Portugal
2022/12/09 Novel phage-derived product to control Paenibacillus larvae in bee hives
(Thesis) Arguer
 Henrique Gonçalves Ribeiro (PhD)
Universidade do Minho Centro de Engenharia Biológica, Portugal
2022/02/18 Phage-encoded proteins for Helicobacter pylori detection
(Thesis) Main arguer
 Marta Daniela Coelho Barbosa (Master)
Universidade do Minho Centro de Engenharia Biológica, Portugal
2020/07/17 Design of NAM-probes specific to Legionella spp.
Supervisor
 Inês Armelim (Master)
Universidade do Porto Faculdade de Engenharia, Portugal
2017/10/11 Product of Biomode based in the PNA-FISH technology for the detection of Aspergillus fumigatus in the clinical area
Supervisor
 Sara Moura (Master)
Universidade de Aveiro Departamento de Química, Portugal
2016/10 Development and validation of a LNA-FISH method for the detection of Aeromonas
(Thesis) Main arguer
 Cátia Filipa Magalhães Ferreira (Master)
Universidade do Minho Departamento de Engenharia Biológica, Portugal
2016/10 Development of a phage-based lab-on-chip for the detection of foodborne pathogens
(Thesis) Main arguer
 Susana Patrícia Chaves Costa (Master)
Universidade do Minho Departamento de Engenharia Biológica, Portugal
2013/10 Interference Studies for a Product based on PNA-FISH
Supervisor
 Teresa Maria Conde Lopes (Master)
Universidade do Minho Departamento de Engenharia Biológica, Portugal

Biomode SA, Portugal
2012/12/07 Development of PNA-FISH methods for the detection of foodborne pathogens
Thesis Member
 Ana Isabel Rocha Fontão (Master)
2012/12/07 Certification of Biotechnological processes and products
Thesis Member
 Vasco Rafael Fernandes dos Santos (Master)

Association member

Society Organization name Role
2021/02 - Current Sociedade Portuguesa de Microbiologia (SPM) Effective member - 681
2020/02 - Current Associação Nacional de Investigadores em Ciência e Tecnologia (Alternate in the list for the General Assembly - year 2022) Member

Committee member

Activity description
Role 		Institution / Organization
2020/09/15 - Current Topical Advisory Panel Member at Micromachines (MDPI)
Other
2022/10/17 - 2022/10/19 Scientific Committee for the conference "Microbiologia 2022". Panel Food Microbiology. https://microbiologia2022.com/
2013 - 2017 AOAC Research Institute Advisory Council (US Association of Analytical Communities)
Advisor / Consultant

Course / Discipline taught

Academic session Degree Subject (Type) Institution / Organization
2021/05/06 - 2021/05/06 Summer School 2 "FISHing Bacteria" - part of the practical classes (Outros) Universidade do Porto Faculdade de Engenharia, Portugal

Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal
2014/04/03 - 2014/04/03 Invited to teach the seminar: “PNA-FISH - Fluorescence in situ hybridization with Peptide Nucleic Acid probes Microorganisms detection” Institution: Faculdade de Ciências da Universidade Fernando Pessoa, Class of Industrial and Pharmaceutical Biotechnology. Mestrado Integrado em Ciências Farmacêuticas (Mestrado integrado) Universidade Fernando Pessoa, Portugal
2013/05/21 - 2013/05/21 Invited to teach the seminar: “PNA-FISH - for Microorganisms detection” Institution: Faculdade de Ciências da Universidade Fernando Pessoa, Class of Industrial and Pharmaceutical Biotechnology. Mestrado Integrado em Ciências Farmacêuticas (Mestrado integrado) Universidade Fernando Pessoa, Portugal
2011/05/12 - 2011/05/13 Practical Classes Support “Third International Practical Course on Biofilm Sciences - Biofilms: from Basic to Emergent” (Outros) Universidade do Minho Departamento de Engenharia Biológica, Portugal
2009/02/20 - 2009/02/21 Practical Classes Support “Second International Practical Course on Biofilm Sciences - Biofilms” (Outros) Universidade do Minho Departamento de Engenharia Biológica, Portugal
2006/09/15 - 2006/09/16 Practical Classes Support “First International Practical Course on Biofilm Sciences - Biofilms (Outros)
2000 - 2001 Support of the Practical classes (during the end of course stage) of the Ecology Discipline Institution: Sciences School, Universidade do Minho, Braga (Portugal) (Licenciatura) Universidade do Minho Departamento de Biologia, Portugal

Evaluation committee

Activity description
Role 		Institution / Organization Funding entity
2022 - Current Guest Associate Editor in Frontiers in Cellular and Infection Microbiology (Clinical Microbiology)
2022 - Current Review Editor - Frontiers in Cellular and Infection Microbiology -Novel diagnostic methods for microbial detection
2022 - Current Review Editor in Frontiers in Food Microbiology
2023/06 - 2023/06 2023 AOAC Eurofins “Testing for Life Student Scholarship” Award - Member of the Judging Panel
Evaluator
 AOAC International, United States Administration on Aging

AOAC International
2023/06 - 2023/06 2023 AOAC Herbalife Student Scholarship Award - Member of the Judging Panel
Evaluator
 AOAC International, United States AOAC International
2022 - 2023/01/15 Guest Editor in the Special Issue "Biomedical Microdevices: State of the Art and Trends". A special issue of Micromachines (ISSN 2072-666X). This special issue belongs to the section "B: Biology and Biomedicine". Deadline for manuscript submissions: 15 Jan 2022 (2 published papers)
Coordinator
 Multidisciplinary Digital Publishing Institute AG, Switzerland
2022/03 - 2022/03 Rapporteur - EIT Health RIS Innovation Call 2022; https://eit.europa.eu/our-activities/outreach/eit-regional-innovation-scheme-ris (3 projects)
2022/03 - 2022/03 EIT Food Business Creation programmes 2022 - online evaluation (10 Projects)
2022/02 - 2022/03 Expert for RIS Innovation Call 2022 Call For Evaluation; https://eithealth.eu/opportunity/eit-health-ris-innovation-call-2022/ (4 projects) Evaluator
Evaluator
 European Institute of Innovation and Technology, Hungary
2020/10/20 - 2021/12/31 Guest Editor in the Special Issue "Microfluidics and Lab-on-a-Chip Applications for Biosensing". A special issue of Micromachines (ISSN 2072-666X). This special issue belongs to the section "B: Biology and Biomedicine". Deadline for manuscript submissions: 31 Dec 2021. (5 published papers)
Coordinator
 Multidisciplinary Digital Publishing Institute AG, Switzerland
2021/01/19 - 2021/01/29 Rapporteur - EIT Health RIS Innovation Call 2021; https://eit.europa.eu/our-activities/outreach/eit-regional-innovation-scheme-ris (2 projects)
Evaluator
 European Institute of Innovation and Technology, Hungary European Institute of Innovation and Technology
2020/12/07 - 2021/01/18 Expert for EIT Health RIS Innovation Call 2021; https://eit.europa.eu/our-activities/outreach/eit-regional-innovation-scheme-ris (2 projects)
Evaluator
 EIT Health, Germany EIT Health
2020/04/09 - 2020/04/10 Evaluator of EIT Health COVID-19 “Rapid Response” in 2020 (2 projects)
Evaluator
 European Institute of Innovation and Technology, Hungary
2020/03 - 2020/03 Rapporteur - RIS 2020 Innovation call; https://eit.europa.eu/our-activities/outreach/eit-regional-innovation-scheme-ris (2 projects)
Evaluator
 European Institute of Innovation and Technology, Hungary
2020/02 - 2020/02 Expert for EIT Health RIS Innovation Call 2020; https://eit.europa.eu/our-activities/outreach/eit-regional-innovation-scheme-ris (4 projects)
Evaluator
 European Institute of Innovation and Technology, Hungary
2019/07 - 2019/07 Expert for EIT Health RIS Innovation Call 2019 https://eit.europa.eu/our-activities/outreach/eit-regional-innovation-scheme-ris (2 projects)
Evaluator
 European Institute of Innovation and Technology, Hungary
2019/06 - 2019/06 Expert for EIT Health Innostars (https://eithealth.eu/) - European Health Catapult Innostars (3 projects).
Evaluator
 European Institute of Innovation and Technology, Hungary
2019/04 - 2019/04 External Expert for EIT Health Innostars (https://eithealth.eu/) - EIT Headstart Innostars Funding Programme (6 projects). European Institute of Innovation and Technology, Hungary
2012/10 - 2012/10 Independent expert for European research and innovation FP7-HEALTH-2013-INNOVATION-2: HEALTH.2013.0-1: Boosting the translation of health research projects' results into innovative applications for health. European Commission, Belgium

Interview (newspaper / magazine)

Activity description Newspaper / Forum
2023/03 e.Biofilm project and my work on Legionella spp. was described at Issue nº 63 of the Engineering Magazine from Faculty of Engineering from University of Porto.
2022 The Young Investigator Award from the Montana State University that I had obtained was noticed at “U.Porto News” website.
2015/03/16 Biomode new investment round - Biomode company Observador
2012/04/02 Biomode: Biotechnology on the way to the USA Dinheiro Vivo

Interview (tv / radio show)

Program Topic
2023/02 - Current "90 Segundos de Ciência" program, a scientific dissemination program coordinated by Joana Lobo Antunes and António Granado, broadcast daily on Antena 1. Legionella Biofilms
2015/04/02 - 2015/04/02 Interview with Porto Canal Porto Canal, "Mentes que Brilham" Biomode

Journal scientific committee

Journal title (ISSN) Publisher
2023/04 - Current JoVE Methods Collection under the Topic “FISH technologies for microbial detection”.
2023 - Current Associate Editor for Microbiotechnology Frontiers in Microbiology
2022 - Current Cellular and Infection Microbiology - Biofilms Frontiers
2021/01/05 - Current International Journal of Molecular Sciences (ISSN 1422-0067) MDPI
2021 - Current Vaccines (2076-393X) MDPI
2021 - Current Microorganisms (2076-2607) MDPI
2020/11/24 - Current Journal of Fungi (ISSN 2309-608X) MDPI
2020/10/16 - 2022/10/25 Foods (ISSN 2304-8158) MDPI
2020/10/21 - 2020/11/11 BMC Microbiology (1471-2180) Springer (Biomed Central Ltd.)
2020/07/22 - 2020/07/31 processes (2227-9717) MDPI
2020/03 - 2020/04 Micromachines (ISSN 2072-666X) (2072-666X) MDPI

Mentoring / Tutoring

Topic Student name
2018 - 2018 IEFP Internship - Market approach (Biomode SA) Elísia Lopes
2012 - 2012 IEFP Internship - Company operations development (Biomode SA) Joana Lima
2012 - 2012 IEFP Internship - Products development (Biomode SA) Rui Rocha
Distinctions

Award

2022 CBE Young Investigator Award - Center for Biofilm Engineering
Montana State University, United States
2017 Second Prize in a Scientific Posters Competition: R. Oliveira, R. Rocha, M. Sousa, L. Cerqueira, Nuno F. Azevedo, C. Almeida. Rapid identification of Campylobacter spp. using PNA FISH in food samples
V AEICBAS Biomedical Congress, 21st to the 23rd of April 2017, Porto, Portugal., Portugal
2012 BES Innovation Award - Panel Natural resources and feeding
Banco Espírito Santo e Comercial de Lisboa, Portugal

Other distinction

2019 Honourable Mention - Best student at General Management Course
Universidade Católica Portuguesa Católica Porto Business School, Portugal
2018 Selected to represent Catolica Business School in the 2nd Edition of the 1st round of the global management challenge
Universidade Católica Portuguesa Católica Porto Business School, Portugal
2014 Series A funds (1.6M€): The spin-off Biomode SA was award with an investment to support the certification and the market entry
2010 Seed funds (300k€): The spin-off Biomode was awarded with an investment (InovCapital-ACTec venture fund) intended to support the products development and certification.
Portugal Ventures, Portugal
2009 CienCinvest financing to perform a Cohort study to validate PNA-FISH method for the detection of H. pylori and clarithromycin resistance (IPO/IPATIMUP/INSA/U.MINHO) (60k€)