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Cláudia Patrícia Almeida Alves. Completed the Doutoramento in Biotecnologia e Biociências in 2021/05/14 by Universidade de Lisboa Instituto Superior Técnico, Mestrado in Mestrado Integrado em Engenharia Biológica in 2014 by Universidade de Lisboa Instituto Superior Técnico and Licenciatura in Mestrado Integrado em Engenharia Biológica in 2013 by Universidade de Lisboa Instituto Superior Técnico. Is Contracted Researcher in Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento. Published 11 articles in journals. Organized 1 event(s). Participated in 2 event(s). Participates and/or participated as PhD Student Fellow in 1 project(s). Works in the area(s) of Engineering and Technology with emphasis on Industrial Biotechnology, Engineering and Technology with emphasis on Industrial Biotechnology with emphasis on Bioprocessing Technologies, Engineering and Technology with emphasis on Industrial Biotechnology with emphasis on Fermentation, Natural sciences with emphasis on Biological Sciences with emphasis on Molecular Biology and Medical and Health Sciences with emphasis on Medical Biotechnology with emphasis on Gene-based Diagnostics and Therapeutic Interventions. In his curriculum Ciência Vitae the most frequent terms in the context of scientific, technological and artistic-cultural output are: Bactérias ácido lácticas; DNA plasmídico; Biologia sintética; Engenharia de estirpes; Celulose; Módulos de Ligação a Carbohidratos; Reconhecimento Biomolecular; Materiais Funcionais Avançados; 5'-UTR; Minicircle; Ribosome binding site; translational initiation rate; mRNA secondary structure; parA resolvase; Gene therapy; Hydrophobic interaction chromatography; Nicking endonuclease; Purification; Multimodal chromatography; Plasmid DNA; Supercoiling; Plasmid isoforms; Separation; minicircle DNA; non-viral DNA vector; biopharmaceuticals; chromatography; B-Z transition; Superhelically induced duplex destabilization; gene therapy; VEGF; angiogenesis; mesenchymal stem/stromal cells (MSC); minicircles; hydrophobic interaction chromatography; minicircle; nicking endonuclease; non-viral vector; purification; .
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Identification

Personal identification

Full name
Cláudia Patrícia Almeida Alves

Citation names

  • Alves, Cláudia
  • Alves, CPA
  • Cláudia P.A. Alves

Author identifiers

Ciência ID
EE1A-A713-343E
ORCID iD
0000-0002-5626-9100
Scopus Author Id
57188665467

Knowledge fields

  • Engineering and Technology - Industrial Biotechnology
  • Engineering and Technology - Industrial Biotechnology - Bioprocessing Technologies
  • Engineering and Technology - Industrial Biotechnology - Fermentation
  • Natural sciences - Biological Sciences - Molecular Biology
  • Medical and Health Sciences - Medical Biotechnology - Gene-based Diagnostics and Therapeutic Interventions

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese (Mother tongue)
English Advanced (C1) Proficiency (C2) Proficiency (C2) Advanced (C1)
Education
Degree Classification
2016/09/12 - 2021/05/14
Concluded
 Biotecnologia e Biociências (Doutoramento)
Universidade de Lisboa Instituto Superior Técnico, Portugal
""Escherichia coli Genome Engineering For Improved Minicircle Production"" (THESIS/DISSERTATION)
 Pass with distinction
2014
Concluded
 Mestrado Integrado em Engenharia Biológica (Mestrado)
Universidade de Lisboa Instituto Superior Técnico, Portugal
"Development of a method for the purification of minicircles" (THESIS/DISSERTATION)
 16
2013
Concluded
 Mestrado Integrado em Engenharia Biológica (Licenciatura)
Universidade de Lisboa Instituto Superior Técnico, Portugal
"n/a" (THESIS/DISSERTATION)
 13
Affiliation

Science

Category
Host institution 		Employer
2024/04/22 - Current Postdoc (Research) FCiênciasID Associação para a Investigação e Desenvolvimento de Ciências, Portugal
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2021/11/01 - 2022/03/31 Contracted Researcher (Research) Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
2015/09/01 - 2016/08/31 Research Assistant (Research) Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
2015/02/01 - 2015/05/31 Research Assistant (Research) Universidade de Lisboa Instituto Superior Técnico, Portugal

Others

Category
Host institution 		Employer
2016/09/01 - 2020/10/31 PhD scholarship Fundação para a Ciência e a Tecnologia, Portugal
Universidade de Lisboa Instituto Superior Técnico, Portugal
2014/12/13 - 2015/01/31 Voluntary fellowship Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
Projects

Grant

Designation Funders
2016/09/01 - 2020/10/31 CRISPR/Cas9-based engineering of Escherichia coli for BioBrick based minicircles
PD/BD/116842/2016
PhD Student Fellow
Universidade de Lisboa Instituto Superior Técnico, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded

Contract

Designation Funders
2018/10/01 - 2022/04/30 Lactic acid bacteria as cell factories: a Synthetic Biology approach for plasmid DNA and recombinant protein production
PTDC/BTM-SAL/28624/2017
Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2018/09/01 - 2021/12/31 Biorecognition as a Tool for the Functionalization of Cellulose-based Materials with Biomolecules and Nanostructures
PTDC/CTM-CTM/30790/2017
Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2019/01/01 - 2019/12/31 Institute for Bioengineering and Biosciences
UID/BIO/04565/2019
Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal

Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
Outputs

Publications

Book chapter
  1. Alves, Cláudia P. A.; Prazeres, Duarte Miguel F.; Monteiro, Gabriel A.. "Real-Time PCR Method for Assessment of ParA-Mediated Recombination Efficiency in Minicircle Production". In Methods in Molecular Biology, 117-131. Springer US, 2023.
    10.1007/978-1-0716-3358-8_10
Conference paper
  1. Ulpiano, Cristiana; Alves, Claudia A. P.; Silva-Santos, A. Rita; Serra, Joana; da Silva, Claudia L.; Monteiro, Gabriel A.. "RNAi as a tool to inhibit the angiogenic potential of human Mesenchymal Stem/Stromal Cells in malignancy". Paper presented in 6th IEEE Portuguese Meeting on Bioengineering (ENBENG), 2019.
    10.1109/enbeng.2019.8692558
Conference poster
  1. Cláudia P.A. Alves; Duarte Miguel F. Prazeres; Gabriel A. Monteiro. "Development of a real-time PCR method to evaluate ParA-mediated recombination efficiency in minicircle production". Paper presented in ESBES 2021, virtual conference, 2021.
  2. Cláudia P.A. Alves; Duarte Miguel F. Prazeres; Gabriel A. Monteiro. "Determination of recombination efficiency by real-time PCR - a strategy to evaluate minicircle production". Paper presented in Microbiotec'19 Congress of Microbiology and Biotechnology, 2019.
  3. Cláudia P.A. Alves; Duarte Miguel F. Prazeres; Gabriel A. Monteiro. "Improving minicircle DNA production by in silico re-design of the ParA resolvase untranslated region". Paper presented in ESBES 2018, 12th European Symposium on Biochemical Engineering Sciences, 2018.
  4. A. Rita Silva-Santos; Cláudia P. A. Alves; Gabriel A. Monteiro; Ana M. Azevedo; Duarte Miguel F. Prazeres. "Multimodal chromatography of supercoiled minicircles: a closer look into DNA-ligand interactions". Paper presented in ESBES 2018, 12th European Symposium on Biochemical Engineering Sciences, 2018.
  5. Cláudia P.A. Alves; Michaela Simcikova; Duarte Miguel F. Prazeres; Gabriel A. Monteiro. "Optimization of ParA resolvase untranslated region - a strategy to boost minicircle DNA production". Paper presented in Microbiotec'17 Congress of Microbiology and Biotechnology, 2017.
  6. Cláudia P.A. Alves; Michaela Simcikova; Liliana Brito; Kristala L. J. Prather; Gabriel A. Monteiro; Duarte Miguel F. Prazeres. "Development of a Nuclease-assisted Method for the Purification of Minicircles". Paper presented in Microbiotec’15 Congress of Microbiology and Biotechnology, 2015.
  7. A. Rita Silva-Santos; Cláudia P.A. Alves; Ana M. Azevedo; Duarte Miguel F. Prazeres. "Separation of plasmid DNA topoisomers by multimodal chromatography". Paper presented in Microbiotec’15 Congress of Microbiology and Biotechnology, 2015.
  8. Sofia O.D. Duarte; Cláudia P.A. Alves; Maria C. Martins; Diana Cipriano; Inês N. Silva; Leonilde M. Moreira; Duarte Miguel F. Prazeres; Gabriel A. Monteiro. "Is increased plasmid DNA production in the Escherichia coli pgi knockout GALG20 strain linked to unintentional genomic deletions?". Paper presented in Microbiotec’15 Congress of Microbiology and Biotechnology, 2015.
  9. Liliana Brito; Michaela Simcikova; Joana Boura; Ana Marisa Salgueiro; Cláudia Alves; António Fiarresga; Cláudia Lobato da Silva; et al. "Transient engineered human Mesenchymal Stem Cells to promote angiogenesis". Paper presented in iMed Lisbon, 2014.
Journal article
  1. Cláudia P.A. Alves; Duarte Miguel F. Prazeres; Gabriel A. Monteiro. "Recombination efficiency measurement by real-time PCR: A strategy to evaluate ParA-mediated minicircle production". Analytical Biochemistry 628 (2021): 114285-114285. https://doi.org/10.1016/j.ab.2021.114285.
    10.1016/j.ab.2021.114285
  2. Serra, Joana; Alves, Cláudia P. A.; Cabral, Joaquim M. S.; Monteiro, Gabriel A.; Silva, Cláudia L.; Prazeres, Duarte Miguel F.. "Minicircle-based expression of vascular endothelial growth factor in mesenchymal stromal cells from diverse human tissues". The Journal of Gene Medicine 23 (2021): e3342. http://dx.doi.org/10.1002/jgm.3342.
    10.1002/jgm.3342
  3. Alves, Cláudia P. A.; Prazeres, Duarte Miguel F.; Monteiro, Gabriel A.. "Minicircle Biopharmaceuticals-An Overview of Purification Strategies". Frontiers in Chemical Engineering 2 (2021): 612594. http://dx.doi.org/10.3389/fceng.2020.612594.
    Open access • Published • 10.3389/fceng.2020.612594
  4. Silva-Santos, A. Rita; Alves, Cláudia P.A.; Monteiro, Gabriel; Azevedo, Ana M.; Prazeres, Duarte Miguel F.. "Multimodal chromatography of supercoiled minicircles: A closer look into DNA-ligand interactions". Separation and Purification Technology 212 (2019): 161-170. http://dx.doi.org/10.1016/j.seppur.2018.11.015.
    Published • 10.1016/j.seppur.2018.11.015
  5. Serra, Joana; Alves, Cláudia P.A.; Brito, Liliana; Monteiro, Gabriel A.; Cabral, Joaquim M.S.; Prazeres, Duarte Miguel F.; da Silva, Cláudia L.. "Engineering of Human Mesenchymal Stem/Stromal Cells with Vascular Endothelial Growth Factor-Encoding Minicircles for Angiogenic Ex Vivo Gene Therapy". Human Gene Therapy 30 3 (2019): 316-329. http://dx.doi.org/10.1089/hum.2018.154.
    Published • 10.1089/hum.2018.154
  6. Alves, Cláudia P. A.; Šimcíková, Michaela; Brito, Liliana; Monteiro, Gabriel A.; Prazeres, Duarte Miguel F.. "Production and Purification of Supercoiled Minicircles by a Combination of In Vitro Endonuclease Nicking and Hydrophobic Interaction Chromatography". Human Gene Therapy Methods 29 4 (2018): 157-168. http://dx.doi.org/10.1089/hgtb.2018.046.
    Published • 10.1089/hgtb.2018.046
  7. Silva-Santos, A. Rita; Alves, Cláudia P.A.; Prazeres, Duarte Miguel F.; Azevedo, Ana M.. "A process for supercoiled plasmid DNA purification based on multimodal chromatography". Separation and Purification Technology 182 (2017): 94-100. http://dx.doi.org/10.1016/j.seppur.2017.03.042.
    Published • 10.1016/j.seppur.2017.03.042
  8. Šimcíková, Michaela; Alves, Cláudia P. A.; Brito, Liliana; Prather, Kristala L. J.; Prazeres, Duarte M. F.; Monteiro, Gabriel A.. "Improvement of DNA minicircle production by optimization of the secondary structure of the 5'-UTR of ParA resolvase". Applied Microbiology and Biotechnology 100 15 (2016): 6725-6737. http://dx.doi.org/10.1007/s00253-016-7565-x.
    Published • 10.1007/s00253-016-7565-x
  9. Alves, Cláudia P.A.; Šimcíková, Michaela; Brito, Liliana; Monteiro, Gabriel A.; Prazeres, Duarte Miguel F.. "Development of a nicking endonuclease-assisted method for the purification of minicircles". Journal of Chromatography A 1443 (2016): 136-144. http://dx.doi.org/10.1016/j.chroma.2016.03.035.
    Published • 10.1016/j.chroma.2016.03.035
  10. Alves, C.P.A.; Duarte, S.O.D.; Monteiro, G.A.; Prazeres, D.M.F.. "Use of an E. coli pgi Knockout Strain as a Plasmid Producer". BioPharm International 29 2 (2016): 38-42. https://www.biopharminternational.com/view/use-e-coli-pgi-knockout-strain-plasmid-producer.
    Open access • Published
  11. Silva-Santos, A.R.; Alves, C.P.A.; Prazeres, D.M.F.; Azevedo, A.M.. "Separation of plasmid DNA topoisomers by multimodal chromatography". Analytical Biochemistry 503 (2016): 68-70. http://www.scopus.com/inward/record.url?eid=2-s2.0-84964681172&partnerID=MN8TOARS.
    Published • 10.1016/j.ab.2016.03.012
Thesis / Dissertation
  1. Alves, CPA. "Escherichia coli genome engineering for improved minicircle production". PhD, Universidade de Lisboa Instituto Superior Técnico, 2021.
  2. Alves, CPA. "Development of a method for the purification of minicircles". Master, Universidade de Lisboa Instituto Superior Técnico, 2014.
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2019 Improving minicircle DNA production in E. coli - in vivo analysis of recombination efficiency after re-design of ParA resolvase 5'-UTR Microbiotec'19 Congress of Microbiology and Biotechnology
(Coimbra, Portugal)
2016 Purification of supercoiled minicircles by a nicking endonuclease-assisted hydrophobic interaction chromatography method BioIberoAmerica
(Salamanca, Spain)

Event organisation

Event name
Type of event (Role) 		Institution / Organization
2017/06/02 - 2018/09/12 Participation in the Organizing Committee of the 12th Symposium of the European Society of Biochemical Engineering Sciences (ESBES2018) (2018/09/09 - 2018/09/12)
Symposium (Member of the Organising Committee)
 Universidade de Lisboa Instituto Superior Técnico, Portugal

Event participation

Activity description
Type of event 		Event name
Institution / Organization
2018/09/18 - 2018/09/22 Cell Culture-based Viral Vaccines Course, 3rd Edition
Workshop
European Society Of Animal Cell Technology, Germany
2017/09/24 - 2017/09/28 Animal Cell Technology course, 7th edition
Workshop
European Society Of Animal Cell Technology, Germany

Jury of academic degree

Topic
Role 		Candidate name (Type of degree)
Institution / Organization
2023/11/28 3D-Printed Matrices for the Purification of Plasmid DNA
(Thesis) Arguer
 Joana da Costa Silva (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal