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Identification

Personal identification

Full name
Américo G Duarte

Citation names

  • G. Duarte, Américo

Author identifiers

Ciência ID
F01D-672C-0C4C
ORCID iD
0000-0003-3855-5231

Email addresses

  • americoduarte@itqb.unl.pt (Professional)

Telephones

Telephone
  • 214469131 (Professional)

Addresses

  • Instituto de Tecnologia Química e Biológica - Universidade Nova de Lisboa. ITQB Av. da República, Estação Agronómica Nacional, 2780, Oeiras, Oeiras, Portugal (Professional)

Knowledge fields

  • Natural sciences - Biological Sciences - Biochemistry
  • Exact Sciences - Chemical Sciences - Electrochemistry
Projects

Contract

Designation Funders
2023/03/01 - Current Electrochemical valorization of CO2 powered by engineered microbial cells
2022.07024.PTDC
Co-Principal Investigator (Co-PI)
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2019/05/01 - Current Unravelling Geobacter sulfurreducens metabolism for bioelectricity production Project
PTDC/BIA-BQM/29118/2017 Principal
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica António Xavier, Portugal
Ongoing
2021/01/01 - 2025/12/31 Life Sciences for a Healthy and Sustainable Future
LA/P/0087/2020
Universidade Nova de Lisboa Microbiologia Molecular Estrutural e Celular, Portugal

Instituto Português de Oncologia de Lisboa Francisco Gentil EPE, Portugal

Fundação Calouste Gulbenkian, Portugal

Instituto Gulbenkian de Ciência, Portugal

Instituto de Biologia Experimental e Tecnológica, Portugal

BioResources 4 Sustainability, Portugal

Universidade NOVA de Lisboa, Portugal

iNOVA4Health, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2018/10 - 2022/10 Unravelling Geobacter sulfurreducens metabolism for bioelectricty production
PTDC/BIA-BQM/29118/2017
 Fundação para a Ciência e a Tecnologia
2019/01/01 - 2019/12/31 Bioresources 4 Sustainability
UID/Multi/04551/2019
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica António Xavier, Portugal

Fundação Calouste Gulbenkian, Portugal

Instituto de Biologia Experimental e Tecnológica, Portugal

BioResources 4 Sustainability, Portugal

Universidade NOVA de Lisboa, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2019/01/01 - 2019/12/31 Molecular, Structural and Cellular Microbiology
UID/CBQ/04612/2019
Universidade NOVA de Lisboa, Portugal

Universidade Nova de Lisboa Microbiologia Molecular Estrutural e Celular, Portugal

Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica António Xavier, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2011/01/01 - 2012/12/31 Strategic Project - LA 6 - 2011-2012
PEst-C/EQB/LA0006/2011
Universidade NOVA de Lisboa Faculdade de Ciências e Tecnologia, Portugal

Universidade do Porto Instituto de Ciências Tecnologias e Agroambiente, Portugal

Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2008/01/01 - 2011/09/30 The two terminal enzymes of denitrification - Nitric oxide reductase and nitrous oxide reductase
PTDC/QUI/64638/2006
Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
Outputs

Publications

Conference abstract
  1. A.C. Pereira, Inês; Barbosa, Ana C. C.; Ferreira, Delfim; Venceslau, Sofia; G. Duarte, Américo. "A molecular view of energy metabolism in anaerobes". Paper presented in The biochemistry Global Summit (25th IUBMB Congress, 46th FEBS congress, 15th PABMB congress, Lisboa, 2022.
    Published
Conference poster
  1. Machado, Nuno; Ines A Cardoso Pereira; G. Duarte, Américo; Conzuelo, F. "Desulfovibrio vulgaris Hildenborough bioelectrodes: a model for CO2 conversion". Paper presented in Bacterial Electron transfer and their regulation, 2024.
  2. Manteigas, Gonçalo; G. Duarte, Américo; A.C. Pereira, Inês. "Role of the electrogenic QrcABCD complex in sulfate and sulfite respiration". Paper presented in 6th International Symposium on Microbial Sulfur Metabolism, 2023.
  3. Catarino, Teresa; G. Duarte, Américo; Pimenta, Andreia I.; Ferreira, Delfim; Barbosa, Ana Catarina; Coito, Ana Margarida; Rebelo Manuel, Rita; et al. "Microbial Physiology and Biocatalysis with Anaerobic Bacteria". Paper presented in 1st LS4Future General Meeting, 2023.
  4. Amaral, Catarina; Morais, Inês; da Silva, Sofia M.; Antunes, Wilson; G. Duarte, Américo; Moe, Elin; Miragaia, Maria ; Serrano, Mónica; Pimentel, Catarina. "From lab to field: low cost saliva tests for mass-screening of SARS-CoV-2". Paper presented in IV International Conference NOVAhealth Chronic Disease and Infection, 2022.
  5. G. Duarte, Américo; I. Pimenta, Andreia; M. Paquete, Catarina; Catarino, Teresa; A.C. Pereira, Inês. "The role of the quinol-interacting ImcH complex on Geobacter sulfurreducens energy conservation and extracellular electron transfer". Paper presented in 21st European Bioenergetics conference EBEC20, 2022.
  6. Cimmino, Lorenzo; Maillard, Julien; G. Duarte, Américo; A.C. Pereira, Inês; Holliger, Christof. "Characterization of the membrane protein complexes responsible for reductive dehalogenation". Paper presented in 21st European Bioenergetics conference EBEC20, 2022.
  7. Pissarro, Teresa; G. Duarte, Américo; Catarina Amaral; Catarina Pimentel; Sofia M. da Silva. "Untangling arsenate response in yeast: characterization of the Acr3 permease". Paper presented in FEBS 2022, 2022.
  8. Pedras, Andreia; Luís, Cláudia; Gaspar-Cordeiro, A.; G. Duarte, Américo; Antunes, Wilson; Lima, Luís M. P.; Amaral, Catarina; Pimentel, Catarina. "Effect of iron on the antifungal activity of caspofungin". Paper presented in TIMB3 Final Meeting, 2022.
  9. Sofia M. da Silva; Pissarro, Teresa; G. Duarte, Américo; Catarina Pimentel. "Unexplored pathways of arsenic detoxification in yeast". Paper presented in Timb3 Final Meeting, 2022.
  10. Manteigas, Gonçalo; G. Duarte, Américo; A.C. Pereira, Inês. "The importance of QrcABCD-QmoABC redox-loop for H2/formate-sulfate respiration in sulfate reducers". Paper presented in Microbiotec2021, 2021.
  11. Mata, Cláudia; da Silva, Sofia; G. Duarte, Américo; Amaral, Catarina; Gonçalves-Pimentel, Catarina. "SYBR Green-based RT-PCR tests for rapid, sensitive and inexpensive detection of SARS-CoV-2". Paper presented in Microbiotec21, 2021.
  12. G. Duarte, Américo; Pimenta, Andreia I.; M. Paquete, Catarina; Domingos, Renato M.; A.C. Pereira, Inês. "The importance of quinol interacting ImcH complex on Geobacter sulfurreducens energy metabolism". Paper presented in Electromicrobiology, 2021.
  13. Cimmino, Lorenzo; G. Duarte, Américo; Schmid, Adrian; A.C. Pereira, Inês; Holliger, Christof; Maillard, Julien. "Absolute quantification of pceABCT gene products and characterization of the membrane protein complex responsible for the reduction of tetrachloroethene in Dehalobacter restrictus". Paper presented in Dehalocon ||| 2020, 2021.
  14. G. Duarte, Américo; Pimenta, Andreia I.; M. Paquete, Catarina; Domingos, Renato M.; A.C. Pereira, Inês. "The role of the quinol dehydrogenase ImcH in Geobacter sulfurreducens energy metabolism". Paper presented in 5th European Meeting of the International Society for Microbial Electrochemistry & Technology, 2021.
  15. G. Duarte, Américo; Pimenta, Andreia I.; M. Paquete, Catarina; Domingos, Renato M.; A.C. Pereira, Inês. "The importance of the ImcH quinol dehydrogenase complex on Geobacter sulfurreducens energy metabolism". Paper presented in Perspectives on Bioenergetics Bioenergetics of the German Biochemical Society (Gesellschaft für Biochemie und Molekularbiologie),, 2021.
  16. Manteigas, Gonçalo; G. Duarte, Américo; A.C. Pereira, Inês. "Sulfate reducers rely on the QrcABCD complex for H2 or formate-based respiration of sulfate". Paper presented in Perspectives on Bioenergetics Bioenergetics of the German Biochemical Society (Gesellschaft für Biochemie und Molekularbiologie),, 2021.
  17. G. Duarte, Américo; Domingos, Renato M.; M. Paquete, Catarina; A.C. Pereira, Inês. "The role of quinone-interacting ImcH in Geobacter sulfurreducens energy metabolism.". Paper presented in World Microbe Forum (American Society of Microbiology & Federation of European Biochemical Society), 2021.
Journal article
  1. Sofia Marques da Silva; Catarina Amaral; Cláudia Malta-Luís; Diana Grilo; Américo G. Duarte; Inês Morais; Gonçalo Afonso; et al. "A one-step low-cost molecular test for SARS-CoV-2 detection suitable for community testing using minimally processed saliva". Biology Methods and Protocols 9 1 (2024): https://novaresearch.unl.pt/en/publications/14f820dc-9fba-4ad8-90ec-9a2a425d474a.
    10.1093/biomethods/bpae035
  2. Anna Aldinio-Colbachini; Alain Grossi; Américo G. Duarte; Jean Vincent Daurelle; Vincent Fourmond. "Combining a Commercial Mixer with a Wall-Tube Electrode Allows the Arbitrary Control of Concentrations in Protein Film Electrochemistry". Analytical Chemistry (2024): https://novaresearch.unl.pt/en/publications/bdf81e17-50a0-4308-b457-9ad117b82956.
    10.1021/acs.analchem.3c05293
  3. Aldinio-Colbachini, Anna; Grossi, Alai ; G. Duarte, Américo; Daurelle, Jean-Vincent; Fourmond, Vincent. "RETURN TO ARTICLES ASAPPREVARTICLENEXT Journal Logo Combining a Commercial Mixer with a Wall-Tube Electrode Allows the Arbitrary Control of Concentrations in Protein Film Electrochemistry". Analytical Chemistry (2024):
    In press
  4. Lorenzo Cimmino; Américo G. Duarte; Dongchun Ni; Babatunde E. Ekundayo; Inês A. C. Pereira; Henning Stahlberg; Christof Holliger; Julien Maillard. "Structure of a membrane-bound menaquinol:organohalide oxidoreductase". Nature Communications (2023): https://doi.org/10.1038/s41467-023-42927-7.
    10.1038/s41467-023-42927-7
  5. Andreia I. Pimenta; Catarina M. Paquete; Leonor Morgado; Marcus J. Edwards; Thomas A. Clarke; Carlos A. Salgueiro; Inês A. C. Pereira; Américo G. Duarte. "Characterization of the inner membrane cytochrome ImcH from Geobacter reveals its importance for extracellular electron transfer and energy conservation". Protein Science (2023): https://doi.org/10.1002/pro.4796.
    10.1002/pro.4796
  6. Morais, Inês; Amaral, Catarina; Luís, Cláudia; da Silva, Sofia M.; Grilo, Diana; G. Duarte, Américo; Afonso, Gonçalo; et al. "A low-cost molecular test for SARS-CoV-2 detection suitable for variant discrimination and community testing using saliva". medRxiv (2023):
    Published
  7. Andreia I. Pimenta; Catarina M. Paquete; Leonor Morgado; Marcus J. Edwards; Thomas Clarke; Carlos A. Salgueiro; In^es A.C. Pereira; Américo G. Duarte. "Role of the inner membrane cytochrome ImcH in Geobacter extracellular electron transfer and energy conservation". bioRxiv (2023): https://www.biorxiv.org/content/early/2023/05/03/2023.05.03.539133.
    10.1101/2023.05.03.539133
  8. Barton, Larry L.; Duarte, Americo G.; Staicu, Lucian C.. "Genomic insight into iron acquisition by sulfate-reducing bacteria in microaerophilic environments". BioMetals 36 2 (2022): 339-350. http://dx.doi.org/10.1007/s10534-022-00410-8.
    10.1007/s10534-022-00410-8
  9. Catarina Amaral; Wilson Antunes; Elin Moe; Américo G. Duarte; Luís M. P. Lima; Cristiana Santos; Inês L. Gomes; et al. "A molecular test based on RT-LAMP for rapid, sensitive and inexpensive colorimetric detection of SARS-CoV-2 in clinical samples". Scientific Reports 11 1 (2021): https://novaresearch.unl.pt/en/publications/c091a9f3-2ccf-45ba-8743-d598d81bf771.
    10.1038/s41598-021-95799-6
  10. Amaral, C.; Antunes, W.; Moe, E.; G. Duarte, Américo; M. P. Lima, L; Santos, C.; L. Gomes, I; et al. "A molecular test based on RT-LAMP for rapid, sensitive and inexpensive colorimetric detection of SARS-CoV-2 in clinical samples". Scientific Reports 11 (2021):
    Published
  11. Américo G. Duarte; Ana C.C. Barbosa; Delfim Ferreira; Gonçalo Manteigas; Renato Domingos; Inês A. C. Pereira. "Redox loops in anaerobic respiration - The role of the widespread NrfD protein family and associated dimeric redox module". Biochimica et Biophysica Acta - Bioenergetics 1862 7 (2021): https://novaresearch.unl.pt/en/publications/76127d04-3b82-4cd6-99b8-23f99f13d702.
    10.1016/j.bbabio.2021.148416
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2024/03 Characterization of electron transfer chains in Geobacter sulfurreducens Bacterial Electron Transfer processes and their regulation
European Federation of Biotechnology (Alicante, Spain)
2023/08 The role of the inner membrane cytochrome ImcH in Geobacter extracellular electron transfer and energy conservation European Meeting of the International Society for Microbial Electrochemistry and Technology (EU-ISMET )
(Wagningen, Netherlands)
2023/05/10 Role of the inner membrane ImcH in Geobacter sulfurreducens energy metabolis SCAN
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica (Lisboa, Portugal)
2022/11/24 Science and how to be a Scientist, Science will move us foward Scientific culture Science Day, at the opening of the Science Clube
AE Aquilino Ribeiro- Oeiras (Lisboa, Portugal)
2022/09/29 The role of quinol-interacting ImcH in Geobacter sulfurreducens energy metabolism , Membrane proteins involved in extracellular electron transfer-Online symposium
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica (Lisboa, Portugal)
2022/09/20 It takes two times two to tango – cryoEM structure resolution of the membrane-bound tetrachloroethene reductive dehalogenase complex 21st European Bioenergetics conference EBEC20
(Aix En Provence, France)
2022/08/20 Energy conserving membrane complexes in prokaryotic respiration 21st European Bioenergetics conference EBEC20
(Aix en-Provence)
2021/09/09 Bioenergetics of dissimilatory sulfate reduction Perspectives on Bioenergetics Bioenergetics of the German Biochemical Society (Gesellschaft für Biochemie und Molekularbiologie),
2020/05 Energy conservation in sulfate reducers through a widespread dimeric redox module SCAN
ITQB-NOVA
2017/01/25 Electron Transfer between the QmoABC Membrane complex and Adenosine 5´-Phosphosulfate Reductase SCAN
ITQB-NOVA

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2023/05/15 - Current Development of electrochemical devices to valorize CO2 reduction
Co-supervisor of Nuno Machado
2022/02/01 - Current New arsenate transporters in yeasts
Co-supervisor of Teresa Pissarro
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2024/03 - 2025/02 Using sulfate reducing bacteria to reduce CO2
Co-supervisor
 Scientific Research Training (Specialization course)
2024/01 - 2024/06 Purification and characterization of a quinone-dependent Hydrogenase from Escherichia coli
Supervisor
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica António Xavier, Portugal
2024/01 - 2024/06 Engineering Desulfovibrio strains for multiple goals
Supervisor
 Biology (Degree)
2021/02/01 - 2022/10/31 Conservation mechanisms in electroactive bacteria
Supervisor
 Post-Doc (Other)
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2022/01 - 2022/05 Expression study of alcohol and aldehyde oxidoreductases in Desulfovibrio vulgaris
Supervisor
 BSc. (Degree)
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2022/01 - 2022/05 Expression and purification of a quinone dependent hydrogenase from Escherichia coli
Supervisor
 BSc. (Degree)
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Event organisation

Event name
Type of event (Role) 		Institution / Organization
2022/09/29 - 2022/09/29 Online Symposium- Membrane Proteins involved in Extracellular Electron Transfer (2022/09/29 - 2022/09/29)
Meeting (Member of the Organising Committee)
 Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Event participation

Activity description
Type of event 		Event name
Institution / Organization
2020/06/18 - 2020/06/18 JobShadowing Online Online informal conversation with HighSchool students to give them perspective of Science in nowadays
Other
 JobShadowing Online
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Jury of academic degree

Topic
Role 		Candidate name (Type of degree)
Institution / Organization
2021/03 Study of the QrcABCD membrane complex involved in energy conservation in gut anaerobes
Supervisor
 Gonçalo Gabriel Pereira Manteigas (Master)
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2021/02/02 Insight into Catalytic Features of Native and Engineered Laccases and Synthesis of Added-Value Compounds
(Thesis) Main arguer
 Ricardo Manuel Garcia Estevinho (Master)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2020/12/15 Insights into determinants of the activity of the flavodiiron NO reductase from Escherichia coli
(Thesis) Main arguer
 Jessica Cândido Soares (Master)
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Committee member

Activity description
Role 		Institution / Organization
2022/05 - Current Khira Amara PhD thesis scientific committee
Member
2020/10 - Current Maria Eduardo Martins PhD Scientific committee
Member
2022/11 - 2025 Member of the ITQB-NOVA Scientific Council as representative of the Biological Chemistry Division
Member
 Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Course / Discipline taught

Academic session Degree Subject (Type) Institution / Organization
2021/07 - Current Lecturing enzyme immobilization in the Biocatalysis curricular unit Sustainable Chemistry (Doutoramento) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2021/03 - Current Lecturing enzyme immobilization in the Biocatalysis curricular unit Biotechnology for Sustainability (Mestrado) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Evaluation committee

Activity description
Role 		Institution / Organization Funding entity
2022/04/22 - 2022/04/24 Portuguese Biology Olympiads. I was responsible for the design, organization and correction of the Biochemistry practical exam. This was one of three exams that selected the Portuguese representatives in the International Biology Olympiads from which two receive a bronze award.
Coordinator
Distinctions

Award

2022 Scientific Research Award in Military Sciences
Estado-Maior-General das Forças Armadas, Portugal