???global.info.a_carregar???
Diana Marques is an IST Bioengeneering PhD student who has a Master's degree in Biotechnology from IST and a Bachelor's degree in Biochemistry from the NOVA School of Science and Technology. Her scientific interests are in sustainable food systems, specifically in the cellular agriculture field. Currently, in her PhD studies, Diana is focused on edible and plant-based materials for scaffolding fabrication, fish cell eletrical stimulation, bioreactors, and fish cell culture, namely, fish adipogenesis and myogenesis. In her master's thesis, Diana developed novel edible vegan bioinks for 3D bioprinting using algae-based materials. This work was published. After that, Diana developed her research in SCERG at iBB, working on the project Algae2Fish, funded by the US non-profit "The Good Food Institute" (GFI), to produce algae- and plant-based scaffolds to fabricate seabass fish fillets using 3D bioprinting and electrospinning techniques. Diana is also recently involved in the projects VitaminSea (funded by GFI), CleanFish (funded by FCT), and FEASTS (funded by European Commission). Diana is the co-founder and co-president of "The University of Lisbon Alt Protein Project," a GFI program that aims to promote education, awareness, and opportunities for students and researchers who work with alternative proteins. Diana is also a co-founder member and the vice president of the fiscal council of the Portuguese Association for the Development of Cellular Agriculture.
Read full resume ↓
Identification

Personal identification

Full name
Diana Marques

Citation names

  • Marques, Diana

Author identifiers

Ciência ID
F31E-E98B-1C0F
ORCID iD
0000-0001-6291-2038

Websites

Knowledge fields

  • Engineering and Technology
  • Agrarian Sciences - Agrarian and Food Biotechnology - Agricultural Biotechnology and Food Biotechnology
  • Engineering and Technology - Industrial Biotechnology - Bio-derived Novel Materials

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese (Mother tongue)
English Upper intermediate (B2) Upper intermediate (B2) Upper intermediate (B2) Upper intermediate (B2) Upper intermediate (B2)
Education
Degree Classification
2022/09/01 - 2026/09/01
Ongoing
 PhD Program on Bioengineering (Doctor)
Major in Systems Bioengineering
Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
"Whole-cut cell-based fish fillet production by cellular agriculture strategies" (THESIS/DISSERTATION)
2019/09/01 - 2021/11/01
Concluded
 Biotechnology (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
"Novel edible, vegan and electroconductive bioinks for 3D Bioprinting " (THESIS/DISSERTATION)
2016/09/01 - 2019/07/01
Concluded
 Biochemistry (Bachelor)
Universidade NOVA de Lisboa Faculdade de Ciências e Tecnologia, Portugal
Affiliation

Science

Category
Host institution 		Employer
2021/12/01 - Current Researcher (Research) Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
Universidade de Lisboa Instituto Superior Técnico, Portugal
Projects

Grant

Designation Funders
2022/12/01 - Current Whole-cut cell-based fish fillet production by cellular agriculture strategies
2022.12900.BD
PhD Student Fellow
Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
 Fundação para a Ciência e a Tecnologia

Contract

Designation Funders
2023/03/01 - 2026/02/28 CleanFish - New strategies for sustainable production of cell-based fish
2022.07677.PTDC
Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal

Instituto Português do Mar e da Atmosfera, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2021/12/01 - 2022/12/01 Algae2Fish - Seabass whole-cut cultured fish fillet production
BL236
Research Fellow
Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
 The Good Food Institute
Ongoing

Other

Designation Funders
2024/01/01 - Current FEASTS - Fostering European Cellular Agriculture for Sustainable Transition Solutions
HORIZON-CL6-2023-FARM2FORK-01
Researcher
European Commission
2023 - Current VitaminSea - Enhancing Cost-Effective and Efficient Delivery in Fish Embryonic Stem Cell Culture Medium Via Functional Microcarriers
VitaminSea
Technical development
Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
 The Good Food Institute
Ongoing
Outputs

Publications

Book chapter
  1. Feddern, Vivian; Bastos, Ana Paula Almeida; Gressler, Vanessa; Marques, Diana M. C.; Ferreira, Frederico C.; Rodrigues, Carlos André Vitorino; Teixeira, Marcus Vinicius Telles; da Silva, Camila Luna. "Cell Lines for Cultivated Meat Production". In Cultivated Meat, 29-54. Springer Nature Switzerland, 2024.
    10.1007/978-3-031-55968-6_3
  2. Feddern, Vivian; de Oliveira, Karla Pollyanna Vieira; Gressler, Vanessa; Fogaça, Fabíola H.S.; de Faria Lopes, Giselle P.; Rodrigues, Carlos A.V.; Marques, Diana M.C.; Sanjuan-Alberte, Paola. "Cultivated poultry and seafood: Opportunities and future challenges". In Cellular Agriculture, 267-292. Elsevier, 2024.
    10.1016/b978-0-443-18767-4.00024-x
Conference poster
  1. Malhão, Beatriz; Pereira, Bernardo; Silva, João Carlos; Rodrigues, Carlos A.V.; Sanjuan Alberte, Paola; Marques, Diana; Frederico Castelo Ferreira. Corresponding author: Marques, Diana. "Edible electrospun plant-based nanofibers for fish cell culture: advancing scaffolds for cultured fish". Paper presented in 3rd International Multidisciplinary Conference on Polymers & Chemistry, 2024.
  2. Marques, Diana; GUSMÃO, AFONSO; Rodrigues, Carlos A.V.; Sanjuan Alberte, Paola; Frederico Castelo Ferreira. Corresponding author: Frederico Castelo Ferreira. "Combining 3D bioprinting and electrospinning as a strategy to produce multi-tissue cultured fish fillets". Paper presented in 2nd International Cellular Agriculture Conference: Bringing Cellular Agriculture to the Consumer, 2024.
  3. Marques, Diana; Rodrigues, Carlos A.V.; Sanjuan Alberte, Paola; Frederico Castelo Ferreira. "Manufacturing cultivated fish fillets combining bioprinting, electrospun fibers, and electrical stimulation". Paper presented in The Good Food Conference 2023: Path to 2030, 2023.
  4. Marques, Diana; Afonso Gusmão; Madalena Jabouille; Schüler, Kristin; Marco Leite; Rodrigues, Carlos A.V.; Sanjuan Alberte, Paola; Frederico Castelo Ferreira. Corresponding author: Frederico Castelo Ferreira. "3D bioprinting of novel edible bioinks for cultured fish muscle tissue". Paper presented in 1st International Conference of the Portuguese Association for Cellular Agriculture, 2023.
  5. GUSMÃO, AFONSO; Marques, Diana; Madalena Jabouille; Leite, Marco; Sanjuan Alberte, Paola; Frederico Castelo Ferreira. "Designing, prototyping and validation of an affordable 3D bioprinter for cultured fish fillets manufacture". Paper presented in 1st International Conference of the Portuguese Association for Cellular Agriculture, 2023.
  6. Bernardo Pereira; Marques, Diana; Fábio F. F. Garrudo; Silva, João Carlos. "Electrospun edible fibers: novel scaffolds for cultured fish fillets". Paper presented in 1st International Conference of the Portuguese Association for Cellular Agriculture, 2023.
  7. Jabouille, Madalena; Marques, Diana; Schüler, Kristin; GUSMÃO, AFONSO; Sanjuan-Alberte, Paola; Frederico Castelo Ferreira. "3D bioprinting of novel edible fat (bio)inks for enhanced fat tissue in cultured fish fillets". Paper presented in 1st International Conference of the Portuguese Association for Cellular Agriculture, 2023.
  8. Marques, Diana; Rodrigues, Carlos A.V.; Sanjuan Alberte, Paola; Frederico Castelo Ferreira. "Additive manufacturing approaches for fish cellular agriculture". Paper presented in Encontro Ciência 2023, 2023.
  9. Alves, Laura; Marques, Diana; Nogueira, Diogo E.S.; Salvador, William O.S.; Sanjuan-Alberte, Paola; Joaquim M.S. Cabral; Ferreira, Frederico; Duscher, Alexandrea ; Rodrigues, Carlos A.V.. Corresponding author: Rodrigues, Carlos A.V.. "Sea Bass Embryonic Cell Culture Using Microcarriers for Cultivated Seafood Production". Paper presented in 1st International Cellular Agriculture Conference, 2023.
  10. Bernardo Pereira; Marques, Diana; Rodrigues, Carlos A.V.; Sanjuan Alberte, Paola; Frederico Castelo Ferreira; João Carlos Silva. Corresponding author: Frederico Castelo Ferreira. "Novel electrospun fibers for cultured fish fillets: exploring topological features to guide cellular alignment". Paper presented in 1st International Cellular Agriculture Conference: harnessing biotechnology for food production, 2023.
Journal article
  1. Kristin Schüler; Diana M.C. Marques; Afonso Gusmão; Madalena Jabouille; Marco Leite; Joaquim M.S. Cabral; Paola Sanjuan-Alberte; Frederico Castelo Ferreira. "3D printing of plant-based fat inks towards manufacturing complex cellular agriculture products with fatty structures". Food Hydrocolloids (2024): https://doi.org/10.1016/j.foodhyd.2024.110369.
    10.1016/j.foodhyd.2024.110369
  2. Claire Bomkamp; Lisa Musgrove; Diana M. C. Marques; Gonçalo F. Fernando; Frederico C. Ferreira; Elizabeth A. Specht. "Differentiation and Maturation of Muscle and Fat Cells in Cultivated Seafood: Lessons from Developmental Biology". Marine Biotechnology (2023): https://doi.org/10.1007/s10126-022-10174-4.
    10.1007/s10126-022-10174-4
  3. Afonso gusmão. "Designing and Prototyping a 3D Printer for Multi-Extrusion of Thermo-and Photocurable Hydrogels: Enabling Affordable and Wider Access to Bioprinting". Engineering Archive (2023):
    Open access • Submitted
  4. Marques, Diana; Frederico Castelo Ferreira; Alberte, Paola. Corresponding author: Frederico Castelo Ferreira. "3D Bioprinting of Novel k-Carrageenan Bioinks: An Algae-Derived Polysaccharide". MDPI Bioengineering 9 3 (2022): https://www.mdpi.com/2306-5354/9/3/109.
Preprint
  1. Lisa Schenzle; Kristina Egger; Bernhard Spangl; Mohamed Hussein; Atefeh Ebrahimian; Harald Kuehnel; Frederico C. Ferreira; et al. "Never let me down: new possibilities for lowering serum free cultivation media costs". 2022. https://doi.org/10.1101/2022.11.13.516330.
    10.1101/2022.11.13.516330
Thesis / Dissertation
  1. "Novel edible, vegan and electroconductive bioinks for 3D Bioprinting". Master, Universidade de Lisboa Instituto Superior Técnico, 2021.
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2024/11/16 Combining electrospun plant-based nanofibers with muscle and fat bioinks: a novel approach to produce cultured fish fillets International Scientific Conference on Cultured Meat
(Maastricht, Netherlands)
2024/11/03 Combining 3D bioprinting, electrospinning, and electrical stimulation to produce cultured fish fillets Workshop - The Food of the Future
ITQB (Oeiras, Portugal)
2024/10/04 Combining 3D bioprinting and electrospinning as a strategy to produce multi-tissue cultured fish fillets 2nd International Conference of the Portuguese Association for Cellular Agriculture
CellAgri Portugal (Lisbon, Portugal)
2024/06/18 Cultured fish production - a novel perspective on cellular food? Rede Mar Summer Courses
Universidade Lisboa (Portugal)
2024/05/08 Criar estruturas comestíveis, vegetais e condutoras a partir de materiais naturalmente disponíveis para desenvolver células musculares 10º Edição do Fórum de Engenharia Química e Biológica
Instituto Superior de Engenharia de Lisboa (ISEL) (Lisbon, Portugal)
2023/10/17 The Next-Generation of Fish Fillets: Combining Bioprinting, Electrospinning, and Electrical Stimuli International Scientific Conference on Cultured Meat
University of Maatsrich (Maatsrich, Netherlands)
2023/09/08 Electrical Stimulation in cultured meat: a strategy to enhance myogenesis 1st International Conference of the Portuguese Association for Cellular Agriculture
International Iberian Nanotechnology Laboratory (Braga, Portugal)
2023/06/15 Cellular fish fillets made by 3D bioprinting of novel edible bioinks: enhancing nutritional and organoleptic value 1st International Cellular Agriculture Conference
VTT (Helsinki , Finland)
2023/02/14 Materiais como ingredientes: serão eles peças essenciais para bioimprimir filetes de peixe? Jornadas Tecnológicas 2023 (JORTEC)
JORTEC Departamento de Ciências dos Materiais (Almada, Portugal)
2022/10 Novel edible scaffolds for 3D bioprinting of cell-cultured sea bass fillets Soapbox Science
Festival Internacional de Ciência (FIC.A) (Oeiras, Portugal)
2022/03/28 Cellular Agriculture - Laboratory Food Production Vll Semana da Bioengenharia
Instituto Superior Técnico (Lisboa, Portugal)
2022/02/17 Cellular agriculture JorTec Bioquímica FCT-UNL
Faculdade Ciências e Tecnologia, NOVA (Caparica, Portugal)

Event organisation

Event name
Type of event (Role) 		Institution / Organization
2024/10/02 - 2024/11/20 Alt Protein Fellowship 2024: this fellowship is designed to offer an in-depth exploration of the world of alternative proteins. It’s a blend of self-study and interactive discussions, aimed at providing an intermediate understanding of the science, technology, and impact of alternative protein sources. The curriculum spans an array of topics, from plant-based proteins and fermentation processes to the nuances of cultivated meats and more. (2024/10/02 - 2024/11/20)
Other (Co-organisor)
 Universidade de Lisboa, Portugal

Brown University, United States

Tufts University, United States

University of Illinois Urbana-Champaign, United States

University of Wisconsin-Madison Biotechnology Center, United States
2024/10/04 - 2024/10/05 2nd International Conference of the Portuguese Association for Cellular Agriculture (2024/10/04 - 2024/10/05)
Conference (Member of the Organising Committee)
 Associação Portuguesa para o Desenvolvimento da Agricultura Celular, Portugal
2023/01/01 - 2023/09/09 1st International Conference of the Portuguese Association for Cellular Agriculture - with the goal to foster and encourage the collaborative development of cellular agriculture within Portugal, while also sharing the latest scientific and innovative advancements related to cellular agriculture products, such as cultivated meat, fish, milk, collagen, leather, fur, wool, and hair, both nationally and internationally. (2023/09/07 - 2023/09/08)
Conference (Member of the Organising Committee)
 Associação Portuguesa para o Desenvolvimento da Agricultura Celular, Portugal

Event participation

Activity description
Type of event 		Event name
Institution / Organization
2024/10/27 - Current Workshop "Um Peixe Bem Impresso, Por Favor" - Noite Europeia dos Investigadores
Workshop
 Um Peixe Bem Impresso, Por Favor
Pavilhão do Conhecimento-Centro Ciência Viva, Portugal
2024/04/22 - 2024/04/26 lX Semana da Bioengenharia IST - Pitch Presentation
Congress
2023/11/27 - 2023/11/29 PhD Open Days 2023
Congress
Universidade de Lisboa Instituto Superior Técnico, Portugal
2023/09/18 - 2023/09/19 Gathering of global students aiming to promote alternative proteins whitin their universities. Roundtables, discussions, field-trips, and networking events.
Conference
 The Alt Protein Project Global Student Summit
The Good Food Institute, United States
2023/05/11 - 2023/05/12 EFSA's Scientific Colloquium 27: Cell culture-derived foods and food ingredients The Colloquium aimed to: -Identify sectors in the agri-food system relevant to cell culture-derived foods and food ingredients; -Review the state of the art of relevant concepts, technologies, and derived products; -Discuss emerging safety and methodological aspects and their impact on EFSA¿s risk assessment approaches.
Congress
 EFSA's Scientific Colloquium 27: Cell culture-derived foods and food ingredients
European Food Safety Authority, Italy
2023/03/22 - 2023/03/22 Alumni Session - Semana da Bioengenharia/ Bioengineering Week
Symposium
 Semana da Bioengenharia/ Bioengineering Week
Universidade de Lisboa Instituto Superior Técnico, Portugal
2021/08 - 2021/08 Oral talk about the development of novel edible, vegetable, and conductive scaffolds from naturally available materials to grow cell-based fish using 3D bioprinting.
Conference
 Industrializing Cell-Based Meats & Seafood

Association member

Society Organization name Role
2023/09/01 - Current The University of Lisbon Alt Protein Project Co-founder & Co-president
2022 - Current CellAgri Portugal - Associação Portuguesa para o Desenvolvimento da Agricultura Celular/ Portuguese Association for the Development of Cellular Agriculture Co-founder member and Vice-President of the Fiscal Council

Interview (newspaper / magazine)

Activity description Newspaper / Forum
2023/09/19 Entrevista para o jornal Público: "Estes investigadores portugueses imprimiram o primeiro filete de robalo em 3D. Pronto para provar? A textura é semelhante à de um robalo de mar. Só este que ainda não está pronto a ser consumido — só em 2026. Até lá, testa-se o mesmo processo com células da cavala." Público
2021/12/27 Production of seabass fillets in vitro using algae- and plant-based materials Público

Interview (tv / radio show)

Program Topic
2023/05/25 - Current Reportagem no programa Linha da Frente 2023 na RTP "Somos o que imprimimos". Já alguma vez imaginou comer um filete de peixe produzido em laboratório e a partir de uma impressão 3D? RTP
2022/01 - Current Primeiro Jornal (Television news) In-vitro production of seabass fillets
2022/05/29 - 2022/05/29 Program Scroll at RTP - Television debate/conversation "Food For Thought" "Food For Thought": Alimentação
Distinctions

Award

2024 Best Poster Presentation at the 3rd International Conference on Chemistry, CHEMEET 2024
2023 3rd place at the PhD Open Days 2023 Pitch Competition
Universidade de Lisboa Instituto Superior Técnico, Portugal
2019 Honorable Mention by Armilar Ventures
Universidade de Lisboa Instituto Superior Técnico, Portugal