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Identification

Personal identification

Full name
David Pamies

Citation names

  • Pamies, David

Author identifiers

Ciência ID
FB1A-594B-8A48
ORCID iD
0000-0002-1224-573X
Affiliation

Science

Category
Host institution 		Employer
2018/01/01 - Current Principal Investigator (Research) Universite de Lausanne, Switzerland
Projects

Grant

Designation Funders
2022/09 - 2025/09 Next-generation human iPSC-derived 3D brain model to quantify chemical-induced effects on myelin.
407940_206450
 Schweizerischer Nationalfonds zur Förderung der wissenschaftlichen Forschung
2020/01 - 2021/01 A 3D human iPSC-derived brain model coupled to multi-well microelectrode arrays (MEA) to improve in vitro neurotoxicity screening
#19C172
 Novartis Stiftung für Medizinisch-Biologische Forschung
2020/01 - 2020/12 Evaluation of multi-well microelectrode arrays (MEA) for neurotoxicity screening in a 3D human brain iPSC-derived model using a chemical training set
NA
Integration into Research Grant Fellow
Ongoing
2019/01 - 2019/12 Quantitative evaluation of the Key Events Relationships (KERs) resulting in impairment of learning and memory abilities (OECD AOP13) to support regulatory decision-making
NA
Integration into Research Grant Fellow
Concluded
2011/09 - 2017/03 Mapping the Human Toxome by Systems Toxicology
grant.R01ES020750
 National Institute of Environmental Health Sciences
2016 - 2017 A 3D in vitro BBB- microphysiological system to study cerebrovascular perturbation
NA
2012/07 - 2015/06 A 3D Model of Human Brain Development for Studying Gene/Environment Interactions
grant.U18TR000547
 National Center for Advancing Translational Sciences
Outputs

Publications

Book
  1. Martínez, C.E.; Pamies, D.; Sogorb, M.A.; Vilanova, E.. OECD Guidelines and Validated Methods for in Vivo Testing of Reproductive Toxicity. 2011.
    10.1016/B978-0-12-382032-7.10010-4
  2. Pamies, D.; Martínez, C.E.; Sogorb, M.A.; Vilanova, E.. Mechanism-Based Models in Reproductive and Developmental Toxicology. 2011.
    10.1016/B978-0-12-382032-7.10011-6
Journal article
  1. Nancy B Hopf; Laura Suter-Dick; Jörg Huwyler; Myriam Borgatta; Lucie Hegg; David Pamies; Hélène Paschoud; et al. "Novel Strategy to Assess the Neurotoxicity of Organic Solvents Such as Glycol Ethers: Protocol for Combining In Vitro and In Silico Methods With Human-Controlled Exposure Experiments". JMIR Research Protocols (2024): https://doi.org/10.2196/50300.
    10.2196/50300
  2. David Pamies; Tatjana Vujic; Domitille Schvartz; Julien Boccard; Cendrine Repond; Carolina Nunes; Serge Rudaz; et al. "Digoxin Induces Human Astrocyte Reaction In Vitro". Molecular Neurobiology (2023): https://doi.org/10.1007/s12035-022-03057-1.
    10.1007/s12035-022-03057-1
  3. Megan Chesnut; Hélène Paschoud; Cendrine Repond; Lena Smirnova; Thomas Hartung; Marie-Gabrielle Zurich; Helena T. Hogberg; David Pamies. "Human IPSC-Derived Model to Study Myelin Disruption". International Journal of Molecular Sciences 22 17 (2021): 9473-9473. https://doi.org/10.3390/ijms22179473.
    10.3390/ijms22179473
  4. Megan Chesnut; Thomas Hartung; Helena Hogberg; David Pamies. "Human Oligodendrocytes and Myelin In Vitro to Evaluate Developmental Neurotoxicity". International Journal of Molecular Sciences 22 15 (2021): 7929-7929. https://doi.org/10.3390/ijms22157929.
    10.3390/ijms22157929
  5. Qinjie Zhou; Diego F. Niño; Yukihiro Yamaguchi; Sanxia Wang; William B. Fulton; Hongpeng Jia; Peng Lu; et al. "Necrotizing enterocolitis induces T lymphocyte–mediated injury in the developing mammalian brain". Science Translational Medicine (2021): https://doi.org/10.1126/scitranslmed.aay6621.
    10.1126/scitranslmed.aay6621
  6. David Pamies; Marie-Gabrielle Zurich; Thomas Hartung. "Organotypic Models to Study Human Glioblastoma: Studying the Beast in Its Ecosystem". iScience 23 10 (2020): 101633-101633. https://doi.org/10.1016/j.isci.2020.101633.
    10.1016/j.isci.2020.101633
  7. Zhong, Xiali; Harris, Georgina; Smirnova, Lena; Zufferey, Valentin; Sá, Rita de Cássia da Silveira e; Baldino Russo, Fabiele; Baleeiro Beltrao Braga, Patricia Cristina; et al. "Antidepressant Paroxetine Exerts Developmental Neurotoxicity in an iPSC-Derived 3D Human Brain Model". Frontiers in Cellular Neuroscience 14 (2020): http://dx.doi.org/10.3389/fncel.2020.00025.
    10.3389/fncel.2020.00025
  8. Pamies, David. "Good Cell and Tissue Culture Practice 2.0 (GCCP 2.0) – Draft for Stakeholder Discussion and Call for Action". ALTEX (2020): http://dx.doi.org/10.14573/altex.2007091.
    10.14573/altex.2007091
  9. Pamies, David. "A Human iPSC-derived 3D platform using primary brain cancer cells to study drug development and personalized medicine". Scientific Reports (2019): http://dx.doi.org/10.1038/s41598-018-38130-0.
    10.1038/s41598-018-38130-0
  10. Pamies, David. "Suitability of 3D human brain spheroid models to distinguish toxic effects of gold and poly-lactic acid nanoparticles to assess biocompatibility for brain drug delivery". Particle and Fibre Toxicology (2019): http://dx.doi.org/10.1186/s12989-019-0307-3.
    10.1186/s12989-019-0307-3
  11. Chesnut, Megan; Muñoz, Laura S.; Harris, Georgina; Freeman, Dana; Gama, Lucio; Pardo, Carlos A.; Pamies, David. "In vitro and in silico Models to Study Mosquito-Borne Flavivirus Neuropathogenesis, Prevention, and Treatment". Frontiers in Cellular and Infection Microbiology 9 (2019): http://dx.doi.org/10.3389/fcimb.2019.00223.
    10.3389/fcimb.2019.00223
  12. Pamies, David. "Microglia Increase Inflammatory Responses in iPSC-Derived Human BrainSpheres". Frontiers in Microbiology (2018): http://dx.doi.org/10.3389/fmicb.2018.02766.
    10.3389/fmicb.2018.02766
  13. Harris G; Eschment M; Orozco SP; McCaffery JM; Maclennan R; Severin D; Leist M; et al. "Toxicity, recovery, and resilience in a 3D dopaminergic neuronal in vitro model exposed to rotenone.". Archives of toxicology (2018): http://europepmc.org/abstract/med/29955902.
    10.1007/s00204-018-2250-8
  14. Pamies D; Bal-Price A; Chesné C; Coecke S; Dinnyes A; Eskes C; Grillari R; et al. "Advanced Good Cell Culture Practice for human primary, stem cell-derived and organoid models as well as microphysiological systems.". ALTEX (2018): http://europepmc.org/abstract/med/29697851.
    10.14573/altex.1710081
  15. Pamies D; Block K; Lau P; Gribaldo L; Pardo CA; Barreras P; Smirnova L; et al. "Rotenone exerts developmental neurotoxicity in a human brain spheroid model.". Toxicology and applied pharmacology (2018): http://europepmc.org/abstract/med/29428530.
    10.1016/j.taap.2018.02.003
  16. Pamies, D.; Barreras, P.; Block, K.; Makri, G.; Kumar, A.; Wiersma, D.; Smirnova, L.; et al. "A human brain microphysiological system derived from induced pluripotent stem cells to study neurological diseases and toxicity". Altex 34 3 (2017): 362-376. http://www.scopus.com/inward/record.url?eid=2-s2.0-85026243203&partnerID=MN8TOARS.
    10.14573/altex.1609122
  17. Pamies, D.; Bal-Price, A.; Simeonov, A.; Tagle, D.; Allen, D.; Gerhold, D.; Yin, D.; et al. "Good cell culture practice for stem cells & stem-cell-derived models". Altex 34 1 (2017): 95-132. http://www.scopus.com/inward/record.url?eid=2-s2.0-85012027021&partnerID=MN8TOARS.
    10.14573/altex.1607121
  18. Pamies, D.; Hartung, T.. "21st century cell culture for 21st century toxicology". Chemical Research in Toxicology 30 1 (2017): 43-52. http://www.scopus.com/inward/record.url?eid=2-s2.0-85015134000&partnerID=MN8TOARS.
    10.1021/acs.chemrestox.6b00269
  19. Eskes, C.; Boström, A.-C.; Bowe, G.; Coecke, S.; Hartung, T.; Hendriks, G.; Pamies, D.; Piton, A.; Rovida, C.. "Good cell culture practices & in vitro toxicology". Toxicology in Vitro 45 (2017): 272-277. http://www.scopus.com/inward/record.url?eid=2-s2.0-85019569593&partnerID=MN8TOARS.
    10.1016/j.tiv.2017.04.022
  20. Zander, N.E.; Piehler, T.; Hogberg, H.; Pamies, D.. "Explosive Blast Loading on Human 3D Aggregate Minibrains". Cellular and Molecular Neurobiology 37 7 (2017): 1331-1334. http://www.scopus.com/inward/record.url?eid=2-s2.0-85009834050&partnerID=MN8TOARS.
    10.1007/s10571-017-0463-7
  21. Pistollato F; Ohayon EL; Lam A; Langley GR; Novak TJ; Pamies D; Perry G; et al. "Alzheimer disease research in the 21st century: past and current failures, new perspectives and funding priorities.". Oncotarget (2016): http://europepmc.org/abstract/med/27229915.
    10.18632/oncotarget.9175
  22. Kilic, O.; Pamies, D.; Lavell, E.; Schiapparelli, P.; Feng, Y.; Hartung, T.; Bal-Price, A.; et al. "Brain-on-a-chip model enables analysis of human neuronal differentiation and chemotaxis". Lab on a Chip 16 21 (2016): 4152-4162. http://www.scopus.com/inward/record.url?eid=2-s2.0-84992418466&partnerID=MN8TOARS.
    10.1039/c6lc00946h
  23. Pistollato, F.; Ohayon, E.L.; Lam, A.; Langley, G.R.; Novak, T.J.; Pamies, D.; Perry, G.; et al. "Alzheimer disease research in the 21stcentury: Past and current failures, new perspectives and funding priorities". Oncotarget 7 26 (2016): 38999-39016. http://www.scopus.com/inward/record.url?eid=2-s2.0-84982924447&partnerID=MN8TOARS.
  24. Smirnova, L.; Harris, G.; Delp, J.; Valadares, M.; Pamies, D.; Hogberg, H.T.; Waldmann, T.; Leist, M.; Hartung, T.. "A LUHMES 3D dopaminergic neuronal model for neurotoxicity testing allowing long-term exposure and cellular resilience analysis". Archives of Toxicology 90 11 (2016): 2725-2743. http://www.scopus.com/inward/record.url?eid=2-s2.0-84949509769&partnerID=MN8TOARS.
    10.1007/s00204-015-1637-z
  25. Zhu, H.; Bouhifd, M.; Donley, E.; Egnash, L.; Kleinstreuer, N.; Kroese, E.D.; Liu, Z.; et al. "Supporting read-across using biological data". Altex 33 2 (2016): 167-182. http://www.scopus.com/inward/record.url?eid=2-s2.0-84962920734&partnerID=MN8TOARS.
    10.14573/altex.1601252
  26. Ball, N.; Cronin, M.T.D.; Shen, J.; Blackburn, K.; Booth, E.D.; Bouhifd, M.; Donley, E.; et al. "Toward good read-across practice (GRAP) guidance". Altex 33 2 (2016): 149-166. http://www.scopus.com/inward/record.url?eid=2-s2.0-84962861082&partnerID=MN8TOARS.
    10.14573/altex.1601251
  27. Sogorb, M.A.; Pamies, D.; Estevan, C.; Estévez, J.; Vilanova, E.. "Roles of NTE protein and encoding gene in development and neurodevelopmental toxicity". Chemico-Biological Interactions 259 (2016): 352-357. http://www.scopus.com/inward/record.url?eid=2-s2.0-84998812207&partnerID=MN8TOARS.
    10.1016/j.cbi.2016.07.030
  28. Bouhifd, M.; Beger, R.; Flynn, T.; Guo, L.; Harris, G.; Hogberg, H.; Kaddurah-Daouk, R.; et al. "Quality assurance of metabolomics". Altex 32 4 (2015): 319-326. http://www.scopus.com/inward/record.url?eid=2-s2.0-84946731469&partnerID=MN8TOARS.
    10.14573/altex.1509161
  29. Pamies, D.; Vilanova, E.; Sogorb, M.A.. "Functional pathways altered after silencing Pnpla6 (the codifying gene of neuropathy target esterase) in mouse embryonic stem cells under differentiation". In Vitro Cellular and Developmental Biology - Animal 50 3 (2014): 261-273. http://www.scopus.com/inward/record.url?eid=2-s2.0-84898057799&partnerID=MN8TOARS.
    10.1007/s11626-013-9691-4
  30. Dhaouadi, K.; Belkhir, M.; Akinocho, I.; Raboudi, F.; Pamies, D.; Barrajón, E.; Estevan, C.; Fattouch, S.. "Sucrose supplementation during traditional carob syrup processing affected its chemical characteristics and biological activities". LWT - Food Science and Technology 57 1 (2014): 1-8. http://www.scopus.com/inward/record.url?eid=2-s2.0-84894255917&partnerID=MN8TOARS.
    10.1016/j.lwt.2014.01.025
  31. Sogorb, M.A.; Pamies, D.; de Lapuente, J.; Estevan, C.; Este´vez, J.; Vilanova, E.. "An integrated approach for detecting embryotoxicity and developmental toxicity of environmental contaminants using in vitro alternative methods". Toxicology Letters 230 2 (2014): 356-367. http://www.scopus.com/inward/record.url?eid=2-s2.0-84907176470&partnerID=MN8TOARS.
    10.1016/j.toxlet.2014.01.037
  32. Estevan, C.; Fuster, E.; Del Río, E.; Pamies, D.; Vilanova, E.; Sogorb, M.A.. "Organophosphorus pesticide chlorpyrifos and its metabolites alter the expression of biomarker genes of differentiation in D3 mouse embryonic stem cells in a comparable way to other model neurodevelopmental toxicants". Chemical Research in Toxicology 27 9 (2014): 1487-1495. http://www.scopus.com/inward/record.url?eid=2-s2.0-84918567530&partnerID=MN8TOARS.
    10.1021/tx500051k
  33. Pamies, D.; Hartung, T.; Hogberg, H.T.. "Biological and medical applications of a brain-on-a-chip". Experimental Biology and Medicine 239 9 (2014): 1096-1107. http://www.scopus.com/inward/record.url?eid=2-s2.0-84907010370&partnerID=MN8TOARS.
    10.1177/1535370214537738
  34. Leist, M.; Hasiwa, N.; Rovida, C.; Daneshian, M.; Basketter, D.; Kimber, I.; Clewell, H.; et al. "Consensus report on the future of animal-free systemic toxicity testing". Altex 31 3 (2014): 341-356. http://www.scopus.com/inward/record.url?eid=2-s2.0-84904901979&partnerID=MN8TOARS.
    10.14573/altex.1406091
  35. Pamies, D.; Sogorb, M.A.; Fabbri, M.; Gribaldo, L.; Collotta, A.; Scelfo, B.; Vilanova, E.; Harris, G.; Bal-Price, A.. "Genomic and phenotypic alterations of the neuronal-like cells derived from human embryonal carcinoma stem cells (NT2) caused by exposure to organophosphorus compounds paraoxon and mipafox". International Journal of Molecular Sciences 15 1 (2014): 905-926. http://www.scopus.com/inward/record.url?eid=2-s2.0-84892155292&partnerID=MN8TOARS.
    10.3390/ijms15010905
  36. Pamies, D.; Bal-Price, A.; Fabbri, M.; Gribaldo, L.; Scelfo, B.; Harris, G.; Collotta, A.; Vilanova, E.; Sogorb, M.A.. "Silencing of PNPLA6, the neuropathy target esterase (NTE) codifying gene, alters neurodifferentiation of human embryonal carcinoma stem cells (NT2)". Neuroscience 281 (2014): 54-67. http://www.scopus.com/inward/record.url?eid=2-s2.0-84908066521&partnerID=MN8TOARS.
    10.1016/j.neuroscience.2014.08.031
  37. Hogberg, H.T.; Bressler, J.; Christian, K.M.; Harris, G.; Makri, G.; O'Driscoll, C.; Pamies, D.; et al. "Toward a 3D model of human brain development for studying gene/environment interactions". Stem Cell Research and Therapy 4 SUPPL.1 (2013): http://www.scopus.com/inward/record.url?eid=2-s2.0-84891357438&partnerID=MN8TOARS.
    10.1186/scrt365
  38. Pamies, D.; Vicente-Salar, N.; Sogorb, M.A.; Roche, E.. "The effect of CO2 concentration in neuroectoderm commitment of mouse embryonic stem cells". Journal of Histotechnology 36 1 (2013): 11-16. http://www.scopus.com/inward/record.url?eid=2-s2.0-84874773490&partnerID=MN8TOARS.
    10.1179/2046023612Y.0000000018
  39. Dhaouadi, K.; Raboudi, F.; Funez-Gomez, L.; Pamies, D.; Estevan, C.; Hamdaoui, M.; Fattouch, S.. "Polyphenolic Extract of Barbary-Fig (Opuntia ficus-indica) Syrup: RP-HPLC-ESI-MS Analysis and Determination of Antioxidant, Antimicrobial and Cancer-Cells Cytotoxic Potentials". Food Analytical Methods 6 1 (2013): 45-53. http://www.scopus.com/inward/record.url?eid=2-s2.0-84872266719&partnerID=MN8TOARS.
    10.1007/s12161-012-9410-x
  40. Laurenza, I.; Pallocca, G.; Mennecozzi, M.; Scelfo, B.; Pamies, D.; Bal-Price, A.. "A human pluripotent carcinoma stem cell-based model for in vitro developmental neurotoxicity testing: Effects of methylmercury, lead and aluminum evaluated by gene expression studies". International Journal of Developmental Neuroscience 31 7 (2013): 679-961. http://www.scopus.com/inward/record.url?eid=2-s2.0-84887606653&partnerID=MN8TOARS.
    10.1016/j.ijdevneu.2013.03.002
  41. Pallocca, G.; Fabbri, M.; Sacco, M.G.; Gribaldo, L.; Pamies, D.; Laurenza, I.; Bal-Price, A.. "MiRNA expression profiling in a human stem cell-based model as a tool for developmental neurotoxicity testing". Cell Biology and Toxicology 29 4 (2013): 239-257. http://www.scopus.com/inward/record.url?eid=2-s2.0-84883306186&partnerID=MN8TOARS.
    10.1007/s10565-013-9250-5
  42. Pamies, D.; Reig, J.A.; Vilanova, E.; Sogorb, M.A.. "Expression of neuropathy target esterase in mouse embryonic stem cells during differentiation". Archives of Toxicology 84 6 (2010): 481-491. http://www.scopus.com/inward/record.url?eid=2-s2.0-77953027655&partnerID=MN8TOARS.
    10.1007/s00204-010-0518-8
  43. Pamies, D.; Vicente-Salar, N.; Sogorb, M.A.; Roche, E.; Reig, J.A.. "Specific effect of 5-fluorouracil on a-fetoprotein gene expression during the in vitro mouse embryonic stem cell differentiation". International Journal of Toxicology 29 3 (2010): 297-304. http://www.scopus.com/inward/record.url?eid=2-s2.0-77953834499&partnerID=MN8TOARS.
    10.1177/1091581810366312
  44. Sogorb, M.A.; González-González, I.; Pamies, D.; Vilanova, E.. "An alternative in vitro method for detecting neuropathic compounds based on acetylcholinesterase inhibition and on inhibition and aging of neuropathy target esterase (NTE)". Toxicology in Vitro 24 3 (2010): 942-952. http://www.scopus.com/inward/record.url?eid=2-s2.0-77649183580&partnerID=MN8TOARS.
    10.1016/j.tiv.2010.01.008
Preprint
  1. Nancy B Hopf; Laura Suter-Dick; Jörg Huwyler; Myriam Borgatta; Lucie Hegg; David Pamies; Hélène Paschoud; et al. "Novel Strategy to Assess the Neurotoxicity of Organic Solvents Such as Glycol Ethers: Protocol for Combining In Vitro and In Silico Methods With Human-Controlled Exposure Experiments (Preprint)". 2023. https://doi.org/10.2196/preprints.50300.
    10.2196/preprints.50300
Distinctions

Award

2017 Lush - Young Researcher Americas Award
Lush, United Kingdom
2016 SOT Colgate-Palmolive Grant for Alternative Research 2016
Society of Toxicology, United States
2015 Hispanic Organization Of Toxicologists (HOT) travel award
Society of Toxicology, United States
2015 3rd place Neurotoxicology SS Toshio Narahashi Postdoctoral Fellow/Associate Poster Award
Society of Toxicology, United States
2014 In Vitro and Alternative Method Specialty Section Award
Society of Toxicology, United States
2014 MB Research Award
Society of Toxicology, United States
2013 James G. Wilson Presentation Award
2010 ESTIV oral presentation award