???global.info.a_carregar???
Identification

Personal identification

Full name
Michel Kranendonk

Author identifiers

Ciência ID
FC1B-515E-3C03
Education
Degree Classification
1999/04/08
Concluded
 Chemistry (Doctor)
Major in Molecular Toxicology
Vrije Universiteit Amsterdam, Netherlands
"Development of new Escherichia coli mutagenicity test systems. Engineering of cytochrome P450 competent tester bacteria for biotransformation studies of carcinogens" (THESIS/DISSERTATION)
1987
Concluded
 Teaching Certificate in Chemistry (Master)
Vrije Universiteit Amsterdam Afdeling Scheikunde en Farmaceutische Wetenschappen, Netherlands
1987
Concluded
 Biochemistry and Pharmacochemistry (Master)
Major in Biochemistry
Vrije Universiteit Amsterdam Afdeling Scheikunde en Farmaceutische Wetenschappen, Netherlands
"Mating type Switching in the Yeast" (THESIS/DISSERTATION)
1983
Concluded
 Mathematics, Physics and Basic Disciplines in Medicine, (Bachelor)
Vrije Universiteit Amsterdam Afdeling Scheikunde en Farmaceutische Wetenschappen, Netherlands
Affiliation

Science

Category
Host institution 		Employer
2015 - Current Principal Investigator (Research) Universidade Nova de Lisboa Centro de Toxicogenómica e Saúde Humana, Portugal
Universidade Nova de Lisboa, Portugal
2008/12/30 - Current Principal Investigator (Research) Universidade Nova de Lisboa Faculdade de Ciências Médicas, Portugal
2003/01/09 - 2008/12/29 Auxiliary Researcher (Research) Universidade Nova de Lisboa Faculdade de Ciências Médicas, Portugal
1999/01/01 - 2003/01/08 Postdoc (Research) Universidade Nova de Lisboa Faculdade de Ciências Médicas, Portugal
1994 - 1998 Research Trainee (Research) Universidade Nova de Lisboa Faculdade de Ciências Médicas, Portugal
Universidade Nova de Lisboa Faculdade de Ciências Médicas, Portugal

Positions / Appointments

Category
Host institution 		Employer
2024/06 - Current Coordinator Universidade Nova de Lisboa Centro de Toxicogenómica e Saúde Humana, Portugal
Projects

Grant

Designation Funders
2022/12 - 2026/11 Discovering chronic inflammation biomarkers that define key stages in the Healthy-to-NASH (non- alcoholic steatohepatitis) transition to inform early prevention and treatment strategies
101095679
Researcher
Horizon 2020
Ongoing
2014/05/01 - 2018/04/30 Domain Dynamics and Control of ELectron flux
FCT-ANR/BEX-BCM/0002/2013
Principal investigator
Universidade Nova de Lisboa Faculdade de Ciências Médicas, Portugal

Universidade NOVA de Lisboa NOVA Medical School, Portugal

Universidade Nova de Lisboa Centro de Toxicogenómica e Saúde Humana, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2013/04/01 - 2017/02/28 Molecular and Cellular Effects of Human Mutations in Cytochrome P450 Reductase
GM81568-01-04
Researcher
Universidade Nova de Lisboa Faculdade de Ciências Médicas, Portugal
 National Institutes of Health
Concluded
2011/02/01 - 2014/02/28 Bioactivation routes of the anti-HIV drug Nevirapine: identification of reactive metabolites and mutagenic potential
PTDC/QUI-QUI/113910/2009
Researcher
Universidade Nova de Lisboa Faculdade de Ciências Médicas, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2008/04/01 - 2012/02/29 Molecular and Cellular Effects of Human Mutations in Cytochrome P450 Reductase
GM81568-01-04
Researcher
Universidade Nova de Lisboa Faculdade de Ciências Médicas, Portugal
 National Institutes of Health
Concluded
2008/03 - 2012/02 The study of Antley-Bixler Syndrome related mutations of cytochrome P450 oxidoreductase: CYPOR polymorphism and the human cytochrome P450 enzyme complex
PTDC/SAU-GMG/71911/2006
PTDC/SAU-GMG/71911/2006
Principal investigator
Universidade Nova de Lisboa Faculdade de Ciências Médicas, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2000/10/01 - 2002/12/31 DESENVOLVIMENTO POR ENGENHARIA GENÉTICA DE NOVOS SISTEMAS DE TESTES DE MUTAGENICIDADE COM BACTÉRIAS COMPETENTES EM CITOCROMOS P450 HUMANOS
SFRH/BPD/1646/2000
Universidade NOVA de Lisboa NOVA Medical School, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
1999/01/01 - 2000/09/30 DESENVOLVIMENTO POR ENGENHARIA GENÉTICA DE NOVOS SISTEMAS DE TESTES DE MUTAGENICIDADE COM BACTÉRIAS COMPETENTES EM CITOCROMOS P450 HUMANOS
PRAXIS XXI/BPD/18832/98
Universidade NOVA de Lisboa NOVA Medical School, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
1994/12/01 - 1998/11/30 DESENVOLVIMENTO DE NOVOS TESTES BACTERIANOS DE MUTAGENICIDADE EM CÉLULAS CONSTRUÍDAS POR ENGENHARIA GENÉ- TICA E METABOLICAMENTE COMPETENTES
PRAXIS XXI/BD/3374/94
Universidade NOVA de Lisboa NOVA Medical School, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded

Contract

Designation Funders
2019/01/01 - 2019/12/31 Centre for Toxicogenomics and Human Health
UID/BIM/00009/2019
Universidade NOVA de Lisboa, Portugal

Universidade Nova de Lisboa Centro de Toxicogenómica e Saúde Humana, Portugal

Universidade NOVA de Lisboa NOVA Medical School, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2011/01/01 - 2013/12/31 Strategic Project - UI 9 - 2011-2012
PEst-OE/SAU/UI0009/2011
Universidade NOVA de Lisboa NOVA Medical School, Portugal

Universidade Nova de Lisboa Centro de Toxicogenómica e Saúde Humana, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
Outputs

Publications

Book
  1. Amit V. Pandey; Colin J. Henderson; Yuji Ishii; Michel Michel Kranendonk; Wayne L. Backes; Ulrich M. Zanger. Role of Protein-Protein Interactions in Metabolism: Genetics, Structure, Function, 2nd Edition. Lausanne, Switzerland: Frontiers Media SA. 2018.
    Published • 10.3389/978-2-88945-491-4 • Editor
  2. Amit V Pandey; Colin J Henderson; Yuji Ishii; Michel Kranendonk; Wayne L Backes; Ulrich M Zanger. Role of Protein-Protein Interactions in Metabolism: Genetics, Structure, Function. Lausanne, Switzerland: Frontiers Media SA. 2018.
    10.3389/978-2-88945-385-6
Conference abstract
  1. Isabel Barata; Esteves, Francisco; Rueff, José; Kranendonk, Michel. "The role of PGRMC1 in CYP-mediated drug metabolism and its implication in chemotherapy resistance". Paper presented in 3rd NMS Symposium on Chronic Diseases and Translational Science 2021, Lisbon, 2021.
    Published
  2. Inês Saldanha; Esteves, Francisco; Cristina Almeida; Sofia Silva; Rueff, José; Kranendonk, Michel. "CPR protein dynamics and its interaction with microsomal CYPs - the study of caffeine metabolism by CYP1A2". Paper presented in 3rd NMS Symposium on Chronic Diseases and Translational Science 2021, Lisbon, 2021.
    Published
  3. Kranendonk, Michel; Esteves, Francisco; Campelo, Diana; Urban, Philippe; Bozonnet, Sophie; Lautier, Thomas; Rueff, José; Truan, Gilles. "Putative motif in the FMN-domain of human cytochrome P450 oxidoreductase involved in its interaction with structurally diverse redox partner". Paper presented in 21st International Conference on Cytochrome P450: Biochemistry, Biophysics and Biotechnology (ICCP450), Brisbane, 2019.
    Published
  4. Kranendonk, Michel. "Putative motif in the FMN-domain of human cytochrome P450 oxidoreductase involved in its interaction with structurally diverse redox partners". Paper presented in 21th International Conference on Cytochrome P450, Brisbane, 2019.
  5. Kranendonk, Michel. "Cytochrome P450 reductase chimera: turn around the hinge". Paper presented in 19th International Symposium on Flavins and Flavoproteins, Groningen, 2017.
  6. Kranendonk, Michel. "The hinge region of human NADPH-cytochrome P450 reductase in conformational switching: the critical role of ionic strength.". Paper presented in 20th International Conference on Cytochrome P450, Düsseldorf, 2017.
  7. Kranendonk, Michel. "Bacterial Whole-cell Human Cytochrome P450 Activity Assay". Paper presented in 20th International Conference on Cytochrome P450, Düsseldorf, 2017.
  8. Kranendonk, Michel. "Human sulfotransferase 1A1 dependent mutagenicity of Nevirapine metabolite 12OH-NVP". Paper presented in 20th International Symposium on Microsomes and Drug Oxidations, Stuttgart, 2014.
  9. Kranendonk, Michel. "Functional Characterization Of Eight Human CYP1A2 Variants: The Role Of Cytochrome b5". Paper presented in 19th International Symposium on Microsmes and Drug Oxidations/12th European regional ISSX Meeting, Noordwijk aan Zee, 2012.
  10. Kranendonk, Michel. "Altered human CYP3A4 activity caused by Antley Bixler Syndrome-related variants of NADPH-cytochrome P450 oxidoreductase measured in a robust in vitro system". Paper presented in 17th International Conference on Cytochrome P450, Manchester, 2011.
  11. Kranendonk, Michel. "Cytochrome b5 in the CYP enzymatic complex: Prominent role of residue Y181 of CYP-oxidoreductase in the interaction with CYP". Paper presented in 17th International Conference on Cytochrome P450, Manchester, 2011.
  12. Kranendonk, Michel. "Molecular Effects of the Antley-Bixler Syndrome-Associated Mutations A287P and R457H of Human NADPH:P450 Oxidoreductase: Interaction with Redox Partner CYP1A2". Paper presented in 18th International Symposium on Microsomes and Drug Oxidations, Bejing, 2010.
Conference paper
  1. Kranendonk, Michel; Marohnic, Chris C.; McCammon, Karen; Masters, Bettie Sue S.; Rueff, Jose. "Human P450 Reductase Y181D Variant, Molecular Effects of FMN Deficiency". Paper presented in 11th European Regional ISSX Meeting, Lisbon, 2009.
  2. Brito Palma, B.; Sousa e Silva, M; Lastdrager, J.; Rueff, J.; Vermeulen, N.P.E.; Kranendonk, Michel. "Human Cytochrome P450 1A2: Comprehensive Study of Eight Polymorphic Variants". Paper presented in 11th European Regional ISSX Meeting, Lisbon, 2009.
  3. Duarte, MP; Palma, BB; Gilep, AA; Laires, A; Oliveira, JS; Usanov, SA; Rueff, J; Kranendonk, M. "The role of protein-protein interactions in the stimulation of the bioactivation by CYP1A2, 2A6 and 2E1 in the presence of cytochrome b5: A new cell system to study cytochrome P450 mediated biotransformation". Paper presented in 14th International Conference on Cytochromes P450: Biochemistry, Biophysics, and Bioinformatics, Dallas, 2005.
    Published
  4. Duart, M.P.; Brito Palma, B.; Laires, A.; Santos Oliveira, J.; Rueff, J.; Kranendonk, Michel. "BTC, a human P450 containing Escherichia coli tester strain with a high sensitivity towards alkylating agents.". Paper presented in 8th European Regional ISSX Meeting, Dijon, 2003.
  5. Kranendonk, Michel; Mesquita, P.; Vermeulen, N.P.E.; Rueff, J.. "Expression of human cytochrome P450 1A2 in Escherichia coli: A system for biotransformation and genotoxicity studies of chemical carcinogens". Paper presented in 10th International Meeting on Cytochrome P450, Biochemistry, Biophysics and Molecular Biology, San Francisco, 1997.
    10.3390/ecmc2019-06333
  6. Kranendonk, Michel; Mesquita, P.; Laires, A.; Vermeulen, N.P.E.; Rueff, J.. "Expression of human cytochrome P450 1A2 in Escherichia coli: A system for biotransformation and genotoxicity studies of chemical carcinogens.". Paper presented in 7th International Conference on Environmental Mutagens, Toulouse, 1997.
  7. Kranendonk, Michel; RUAS, M; LAIRES, A; RUEFF, J. "DEVELOPMENT AND PREVALIDATION OF AN ESCHERICHIA-COLI TESTER STRAIN FOR GENOTOXINS". Paper presented in Cytochrome P450, Biochemistry, Biophysics and Molecular Biology, Lisbon, 1994.
  8. Kranendonk, Michel; Ruas, M.; Laires, A.; Rueff, J.. "FRAMESHIFT MUTAGENESIS IN ESCHERICHIA COLI". Paper presented in 22nd Annual Meeting of the European Mutagen Society, Berlin, 1993.
    10.1016/0165-1161(93)90163-T
  9. Rueff, J.; Laires, A.; Kranendonk, Michel; Rodrigues, A. S.; Gaspar, J.; Borba, H.; Monteiro, M.. "METABOLIC ACTIVATION OF MUTAGENS BY HUMAN HEMOGLOBIN". Paper presented in 19th Annual Meeting of the European Mutagen Society, Rhodes, 1990.
    10.1016/0165-1161(90)90125-8
Conference poster
  1. Barata, Isabel S; Bruno Costa Gomes; António Sebastão Rodrigues; Rueff, José; Kranendonk, Michel; Esteves, Francisco. "Differential Expression of Phase I Drug Metabolism Enzymes in Doxorubicin Resistant Breast Cancer Cells". Paper presented in 26th Annual Meeting Sociedade Portuguesa de Genética Humana, 2022.
  2. Kranendonk, Michel. "Molecular Insights in the Interaction of Human Cytochrome P450 Oxidoreductase with its Structural Diverse Redox Partners: the Quest of Understanding Genetic Susceptibility". Paper presented in IV Genetics Workshop NOVA-Health, Universidade NOVA de Lisboa, 2019.
  3. Kranendonk, Michel. "The role of Progesterone Receptor Membrane Component 1 (PGRMC1) in cytochromes P450 (CYP)-mediated Xenobiotic metabolism". Paper presented in IV Genetics Workshop NOVA-Health, Universidade NOVA de Lisboa, 2019.
  4. Kranendonk, Michel. "Mutants of Cytochrome P450 Oxidoreductase: Probing the Role of the Hinge Segment in the Interaction with Cytochrome P450s". Paper presented in IV Genetics Workshop NOVA-Health, Universidade NOVA de Lisboa, 2019.
  5. Kranendonk, Michel. "Probing the Role of the Hinge Segment of Cytochrome P450 Oxidoreductase in the Interaction with Cytochrome P450". Paper presented in 9as Jornadas Científicas do IHMT, Instituto de Higiene e Medicina Tropical - Universidade NOVA de Lisboa, 2018.
  6. Kranendonk, Michel. "Probing the Role of the Hinge Segment of Cytochrome P450 Oxidoreductase in the Interaction with Cytochrome P450". Paper presented in 2nd NMS Symposium on Chronic Diseases and Translational Science 2018, 2018.
  7. Kranendonk, Michel. "Initial findings suggest a stimulatory role of Progesterone Receptor Membrane Component 1 (PGRMC1) in Cytochrome P450 (CYP)-mediated metabolism". Paper presented in 2nd NMS Symposium on Chronic Diseases and Translational Science 2018, 2018.
  8. Kranendonk, Michel. "Ionic interactions in the protein dynamics of Cytochrome P450 oxidoreductase". Paper presented in National Science Summit – Ciência 2018, 2018.
  9. Kranendonk, Michel. "Bacterial Whole-cell Human Cytochrome P450 Activity Assay: A high-throughput approach". Paper presented in National Science Summit – Ciência 2018, 2018.
  10. Kranendonk, Michel. "Bacterial Whole-cell Human Cytochrome P450 Activity Assay.". Paper presented in 20th International Conference on Cytochrome P450, 2017.
  11. Kranendonk, Michel. "The hinge region of human NADPH-cytochrome P450 reductase in conformational switching: the critical role of ionic strength". Paper presented in 20th International Conference on Cytochrome P450, 2017.
  12. Kranendonk, Michel. "Cytochrome P450 reductase chimera: turn around the hinge". Paper presented in 19th International Symposium on Flavins and Flavoproteins, 2017.
  13. Kranendonk, Michel. "A região “hinge” da NADPH-citocromo P450 reductase na troca conformacional e o papel crítico da força iónica". Paper presented in 8as Jornadas Científicas do IHMT, Instituto de Higiene e Medicina Tropical - Universidade NOVA de Lisb, 2017.
  14. Kranendonk, Michel. "Development of Human Cytochrome P450 Competent Genotoxicity Tester Bacterial Systems for High Throughput Screening - Functional Characterization of Human Cytochrome P450 1A2 Polymorphic Variants". Paper presented in 3rd Genetics Workshop NOVA-Health, Universidade NOVA de Lisboa, 2017.
  15. Kranendonk, Michel. "Bacterial Whole-cell Human Cytochrome P450 Activity Assay". Paper presented in 3rd Genetics Workshop NOVA-Health, Universidade NOVA de Lisboa, 2017.
  16. Kranendonk, Michel. "The hinge region of human NADPH-cytochrome P450 reductase in conformational switching: the critical role of ionic strength". Paper presented in 3rd Genetics Workshop NOVA-Health, Universidade NOVA de Lisboa, 2017.
  17. Kranendonk, Michel. "Dynamics in NADPH Cytochrome P450 oxidoreductase: Identification of critical protein residues". Paper presented in 7as Jornadas Científicas do IHMT, Universidade NOVA de Lisboa, 2016.
  18. Kranendonk, Michel. "The importance of hinge and FMN genetic variants of NADPH cytochrome P450 oxidoreductase". Paper presented in 2nd Genetics Workshop NOVA-Health, Universidade NOVA de Lisboa, 2016.
  19. Kranendonk, Michel. "Prototype Development of Human Cytochrome P450 competent High Throughput Test for the Detection of Genotoxic/Electrophilic Reactive Metabolites". Paper presented in 1st Genetics Workshop NOVA Health, Universidade NOVA Lisboa, 2015.
  20. Kranendonk, Michel. "Structural features controlling the protein dynamics of NADPH cytochrome P450 oxidoreductase in gated electron transf". Paper presented in 1st Genetics Workshop NOVA Health, Universidade NOVA Lisboa, 2015.
  21. Kranendonk, Michel. "Domain dynamics and control of electron flux of NADPH cytochrome P450 oxidoreductase: Identification of critical residues in the hinge region". Paper presented in 1st Genetics Workshop NOVA Health, Universidade NOVA Lisboa, 2015.
  22. Kranendonk, Michel. "Human sulfotransferase 1A1 dependent mutagenicity of Nevirapine metabolite 12OH-NVP". Paper presented in 20th International Symposium on Microsomes and Drug Oxidations, 2014.
  23. Kranendonk, Michel. "Functional Characterization Of Eight Human CYP1A2 Variants: The Role Of Cytochrome b5". Paper presented in 19th International Symposium on Microsomes and Drug Oxidations / 12th European Regional ISSX Meeting, 2012.
  24. Kranendonk, Michel. "Altered human CYP3A4 activity caused by Antley Bixler Syndrome-related variants of NADPH-cytochrome P450 oxidoreductase measured in a robust in vitro system". Paper presented in 17th International Conference on Cytochromes P450, 2011.
  25. Kranendonk, Michel. "Cytochrome b5 in the CYP enzymatic complex: Prominent role of residue Y181 of CYP-oxidoreductase in the interaction with CYP". Paper presented in 17th International Conference on Cytochromes P450, 2011.
  26. Kranendonk, Michel. "Molecular Effects of the Antley-Bixler Syndrome-Associated Mutations A287P and R457H of Human NADPH:P450 Oxidoreductase: Interaction with Redox Partner CYP1A2". Paper presented in 18th International Symposium on Microsomes and Drug Oxidations, 2010.
  27. Kranendonk, Michel. "NADPH-Cytochrome P450 Reductase Variant Q153R With Apparent Normal, Wild-Type Activities". Paper presented in 11th European Regional ISSX Meeting, 2009.
  28. Kranendonk, Michel. "Human P450 Reductase Y181D Variant, Molecular Effects Of FMN Deficiency". Paper presented in 11th European Regional ISSX Meeting, 2009.
  29. Kranendonk, Michel. "Human Cytochrome P450 1A2: Comprehensive Study of Eight Polymorphic Variants". Paper presented in 11th European Regional ISSX Meeting, 2009.
  30. Kranendonk, Michel. "Hampered FMN Cofactor Binding In the Antley-Bixler Syndrome Variant Y181D of Cytochrome P450 Oxidoreductase". Paper presented in 17th International Symposium on Microsomes and Drug Oxidations, 2008.
  31. Kranendonk, Michel. "Antley-Bixler Syndrome-Related Mutants of Human NADPH-Cytochrome P450 Reductase: Effects on Drug Metabolism and Mutagenesis.". Paper presented in 15th International Conference on Cytochromes P450, 2007.
  32. Kranendonk, Michel. "The Antley-Bixler Syndrome related Y459H mutation of P450 oxidoreductase is seriously hampering the activity of the xenobiotic metabolising enzyme CYP1A2". Paper presented in 16th International Symposium on Microsomes and Drug Oxidations, 2006.
  33. Kranendonk, Michel. "The role of protein-protein interactions in the stimulation of the bioactivation of mutagens by CYP1A2, 2A6 and 2E1 in the presence of cytochrome b5: A new cell system to study the cytochrome P450 mediated biotransformation.". Paper presented in 14th Conference on Cytochrome P450, 2005.
  34. Kranendonk, Michel. "The stimulatory role of human cytochrome b5 in the bioactivation activities of human CYP1A2, 2A6 and 2E1: A new cell-system for the study of human cytochrome P450 mediated biotransformation". Paper presented in 15th International Symposium on Microsomes and Drug Oxidations, 2004.
  35. Kranendonk, Michel. "BTC, a human P450 containing Escherichia coli tester strain with high sensitivity towards alkylating agents". Paper presented in 8th European Regional ISSX Meeting, 2003.
  36. Kranendonk, Michel. "Development of PD1000: A new Escherichia coli strain, highly sensitive towards the detection of alkylating agents". Paper presented in 32nd Annual Meeting of the European Environmental Mutagen Society, 2002.
  37. Kranendonk, Michel. "Escherichia coli MTC, a Human P450 containing in vitro Model for Genotoxicology Studies". Paper presented in 13th International Symposium on Microsomes and Drug Oxidations, 2000.
  38. Kranendonk, Michel. "Escherichia coli MTC, a Human NADPH cytochrome P450 reductase competent mutagenicity tester strain for the expression of human cytochrome P450s". Paper presented in 9th North American ISSX Meeting, 1999.
  39. Kranendonk, Michel. "Escherichia coli BMX100hOR, a P450 oxidoreductase competent mutagenicity tester strain for the expression of human cytochrome P450". Paper presented in 12th International Symposium on Microsomes and Drug oxidations, 1998.
  40. Kranendonk, Michel. "Expression of human cytochrome P450 1A2 in Escherichia coli: A system for biotransformation and genotoxicity studies of chemical carcinogens". Paper presented in 10th International Conference on Cytochrome P450, 1997.
  41. Kranendonk, Michel. "Expression of human cytochrome P450 1A2 in Escherichia coli: A system for biotransformation and genotoxicity studies of chemical carcinogens". Paper presented in 7th International Conference on Environmental Mutagens, 1997.
  42. Kranendonk, Michel. "MX100, an Escherichia coli tester strain for the use in studies of molecular aspects of genotoxic carcinogens". Paper presented in Conference on Molecular Aspects of Carcinogenesis, 1995.
  43. Kranendonk, Michel. "Genotoxicity testing in Escherichia coli: I. Isolation of a tester strain.". Paper presented in 23rd Annual Meeting of the European Environmental Mutagen Society, 1993.
  44. Kranendonk, Michel. "Genotoxicity testing in Escherichia coli: II. Pre-validation of a tester strain". Paper presented in 23rd Annual Meeting of the European Environmental Mutagen Society, 1993.
  45. Kranendonk, Michel. "The isolation and Prevalidation of an Escherichia coli tester strain". Paper presented in 8th International Conference on Cytochrome P450, 1993.
  46. Kranendonk, Michel. "Frameshift-Mutagenesis in E.coli.". Paper presented in 22nd Annual Meeting of the European Environmental Mutagen Society, 1992.
  47. Kranendonk, Michel. "Metabolic activation of mutagens by human haemoglobin". Paper presented in 19th Annual Meeting of the European Environmental Mutagen Society, 1989.
Journal article
  1. Isabel S. Barata; José Rueff; M Kranendonk; Esteves F. "Pleiotropy of Progesterone Receptor Membrane Component 1 in Modulation of Cytochrome P450 Activity.". MDPI (2024):
    10.48350/197179
  2. Francisco Esteves; Cristina M. M. Almeida; Sofia Silva; Inês Saldanha; Philippe Urban; José Rueff; Denis Pompon; Gilles Truan; Michel Kranendonk. "Single Mutations in Cytochrome P450 Oxidoreductase Can Alter the Specificity of Human Cytochrome P450 1A2-Mediated Caffeine Metabolism". Biomolecules (2023): https://doi.org/10.3390/biom13071083.
    10.3390/biom13071083
  3. Barata, Isabel S.; Gomes, Bruno C.; Rodrigues, António S.; Rueff, José; Kranendonk, Michel; Esteves, Francisco. "The Complex Dynamic of Phase I Drug Metabolism in the Early Stages of Doxorubicin Resistance in Breast Cancer Cells". Genes 13 11 (2022): 1977. http://dx.doi.org/10.3390/genes13111977.
    10.3390/genes13111977
  4. Nádia Vital; Célia Ventura; Michel Kranendonk; Maria João Silva; Henriqueta Louro. "Toxicological Assessment of Cellulose Nanomaterials: Oral Exposure". Nanomaterials (2022): https://doi.org/10.3390/nano12193375.
    10.3390/nano12193375
  5. Ana Cabrita; Alexandra M. Medeiros; Telmo Pereira; António Sebastião Rodrigues; Michel Kranendonk; César S. Mendes. "Motor dysfunction in Drosophila melanogaster as a biomarker for developmental neurotoxicity.". iScience (2022): https://doi.org/10.1016/j.isci.2022.104541.
    10.1016/j.isci.2022.104541
  6. Francisco Esteves; José Rueff; Michel Kranendonk. "The Central Role of Cytochrome P450 in Xenobiotic Metabolism—A Brief Review on a Fascinating Enzyme Family". Journal of Xenobiotics (2021): https://doi.org/10.3390/jox11030007.
    10.3390/jox11030007
  7. Célia M. Silveira; Patrícia R. Rodrigues; Wissam Ghach; Sofia A. Pereira; Francisco Esteves; Michel Kranendonk; Mathieu Etienne; M. Gabriela Almeida. "Electrochemical Activity of Cytochrome P450 1A2: The Relevance of O 2 Control and the Natural Electron Donor". ChemElectroChem 8 3 (2021): 430-430. https://doi.org/10.1002/celc.202001615.
    10.1002/celc.202001615
  8. Célia M. Silveira; Patrícia R. Rodrigues; Wissam Ghach; Sofia A. Pereira; Francisco Esteves; Michel Kranendonk; Mathieu Etienne; M. Gabriela Almeida. "Electrochemical Activity of Cytochrome P450 1A2: The Relevance of O 2 Control and the Natural Electron Donor". ChemElectroChem 8 3 (2021): 500-507. https://doi.org/10.1002/celc.202001255.
    10.1002/celc.202001255
  9. Célia M. Silveira; Patrícia R. Rodrigues; Wissam Ghach; Sofia A. Pereira; Francisco Esteves; Michel Kranendonk; Mathieu Etienne; M. Gabriela Almeida. "Front Cover: Electrochemical Activity of Cytochrome P450 1A2: The Relevance of O 2 Control and the Natural Electron Donor (ChemElectroChem 3/2021)". ChemElectroChem 8 3 (2021): 425-425. https://doi.org/10.1002/celc.202001616.
    10.1002/celc.202001616
  10. Francisco Esteves; Philippe Urban; José Rueff; Gilles Truan; Michel Kranendonk. "Interaction Modes of Microsomal Cytochrome P450s with Its Reductase and the Role of Substrate Binding". International Journal of Molecular Sciences (2020): https://doi.org/10.3390/ijms21186669.
    10.3390/ijms21186669
  11. Esteves, Francisco; Campelo, Diana; Gomes, Bruno Costa; Urban, Philippe; Bozonnet, Sophie; Lautier, Thomas; Rueff, José; Truan, Gilles; Kranendonk, Michel. "Corrigendum: The Role of the FMN-Domain of Human Cytochrome P450 Oxidoreductase in Its Promiscuous Interactions With Structurally Diverse Redox Partners". Frontiers in Pharmacology 11 (2020): http://dx.doi.org/10.3389/fphar.2020.00773.
    10.3389/fphar.2020.00773
  12. Esteves, Francisco; Campelo, Diana; Gomes, Bruno Costa; Urban, Philippe; Bozonnet, Sophie; Lautier, Thomas; Rueff, José; Truan, Gilles; Kranendonk, Michel. "The Role of the FMN-Domain of Human Cytochrome P450 Oxidoreductase in Its Promiscuous Interactions With Structurally Diverse Redox Partners". Frontiers in Pharmacology 11 (2020): http://dx.doi.org/10.3389/fphar.2020.00299.
    Open access • Published • 10.3389/fphar.2020.00299
  13. Rueff, J.; Rodrigues, A.S.; Kranendonk, M.. "A personally guided tour on some of our data with the Ames assay—A tribute to Professor Bruce Ames". Mutation Research/Genetic Toxicology and Environmental Mutagenesis 846 (2019): 503094. http://dx.doi.org/10.1016/j.mrgentox.2019.503094.
    10.1016/j.mrgentox.2019.503094
  14. Quast, Robert B.; Fatemi, Fataneh; Kranendonk, Michel; Margeat, Emmanuel; Truan, Gilles. "Accurate Determination of Human CPR Conformational Equilibrium by smFRET Using Dual Orthogonal Noncanonical Amino Acid Labeling". ChemBioChem 20 5 (2019): 659-666. http://dx.doi.org/10.1002/cbic.201800607.
    10.1002/cbic.201800607
  15. Campelo, Diana; Esteves, Francisco; Palma, Bernardo Brito; Gomes, Bruno Costa; Rueff, José; Lautier, Thomas; Urban, Philippe; Truan, Gilles; Kranendonk, Michel. "Probing the Role of the Hinge Segment of Cytochrome P450 Oxidoreductase in the Interaction with Cytochrome P450". International Journal of Molecular Sciences 19 12 (2018): 3914. http://dx.doi.org/10.3390/ijms19123914.
    10.3390/ijms19123914
  16. Francisco Esteves; Diana Campelo; Philippe Urban; Sophie Bozonnet; Thomas Lautier; José Rueff; Gilles Truan; Michel Kranendonk. "Human cytochrome P450 expression in bacteria: Whole-cell high-throughput activity assay for CYP1A2, 2A6 and 3A4". Biochemical Pharmacology 158 (2018): 134-140. https://doi.org/10.1016/j.bcp.2018.10.006.
    10.1016/j.bcp.2018.10.006
  17. Campelo, Diana; Lautier, Thomas; Urban, Philippe; Esteves, Francisco; Bozonnet, Sophie; Truan, Gilles; Kranendonk, Michel. "The Hinge Segment of Human NADPH-Cytochrome P450 Reductase in Conformational Switching: The Critical Role of Ionic Strength (vol 8, 755, 2017)". Frontiers in Pharmacology 9 (2018): http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000427722900001&KeyUID=WOS:000427722900001.
    10.3389/fphar.2018.00175
  18. Pandey, Amit V.; Henderson, Colin J.; Ishii, Yuji; Kranendonk, Michel; Backes, Wayne L.; Zanger, Ulrich M.. "Editorial: Role of Protein-Protein Interactions in Metabolism: Genetics, Structure, Function". Frontiers in Pharmacology 8 (2017): http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000416218000001&KeyUID=WOS:000416218000001.
    10.3389/fphar.2017.00881
  19. Campelo, Diana; Lautier, Thomas; Urban, Philippe; Esteves, Francisco; Bozonnet, Sophie; Truan, Gilles; Kranendonk, Michel. "The Hinge Segment of Human NADPH-Cytochrome P450 Reductase in Conformational Switching: The Critical Role of Ionic Strength". Frontiers in Pharmacology 8 (2017): http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000413932100001&KeyUID=WOS:000413932100001.
    10.3389/fphar.2017.00755
  20. Karen M. McCammon; Satya P. Panda; Chuanwu Xia; Jung-Ja P. Kim; Daniela Moutinho; Michel Kranendonk; Richard J. Auchus; et al. "Instability of the Human Cytochrome P450 Reductase A287P Variant Is the Major Contributor to its Antley-Bixler Syndrome-Like Phenotype". J. Biol. Chem. (2016): jbc.M116.716019-jbc.M116.716019. http://dx.doi.org/10.1074/jbc.m116.716019.
    10.1074/jbc.m116.716019
  21. Bernardo Brito Palma; Daniela Moutinho; Philippe Urban; José Rueff; Michel Kranendonk. "Cytochrome P450 expression system for high-throughput real-time detection of genotoxicity: Application to the study of human CYP1A2 variants". Mutation Research/Genetic Toxicology and Environmental Mutagenesis 806 (2016): 24-33. https://doi.org/10.1016%2Fj.mrgentox.2016.06.004.
    10.1016/j.mrgentox.2016.06.004
  22. Brito Palma, B.; Fisher, C.W.; Rueff, J.; Kranendonk, M.. "Prototype Systems Containing Human Cytochrome P450 for High-Throughput Real-Time Detection of DNA Damage by Compounds That Form DNA-Reactive Metabolites". Chemical Research in Toxicology 29 5 (2016): 747-756. http://www.scopus.com/inward/record.url?eid=2-s2.0-84969835495&partnerID=MN8TOARS.
    10.1021/acs.chemrestox.5b00455
  23. Kranendonk, M.; Alves, M.; Antunes, P.; Rueff, J.. "Human sulfotransferase 1A1-dependent mutagenicity of 12-hydroxy-nevirapine: The missing link?". Chemical Research in Toxicology 27 11 (2014): 1967-1971. http://www.scopus.com/inward/record.url?eid=2-s2.0-84910623665&partnerID=MN8TOARS.
    10.1021/tx5003113
  24. Palma, B.B.; Silva E Sousa, M.; Urban, P.; Rueff, J.; Kranendonk, M.. "Functional characterization of eight human CYP1A2 variants: The role of cytochrome b5". Pharmacogenetics and Genomics 23 2 (2013): 41-52. http://www.scopus.com/inward/record.url?eid=2-s2.0-84873852475&partnerID=MN8TOARS.
    10.1097/FPC.0b013e32835c2ddf
  25. Moutinho, D.; Marohnic, C.C.; Panda, S.P.; Rueff, J.; Masters, B.S.; Kranendonk, M.. "Altered human CYP3A4 activity caused by antley-bixler syndrome-related variants of NADPH-cytochrome P450 oxidoreductase measured in a robust in vitro system". Drug Metabolism and Disposition 40 4 (2012): 754-760. http://www.scopus.com/inward/record.url?eid=2-s2.0-84858412533&partnerID=MN8TOARS.
    10.1124/dmd.111.042820
  26. Gómez-Bombarelli, R.; Palma, B.B.; Martins, C.; Kranendonk, M.; Rodrigues, A.S.; Calle, E.; Rueff, J.; Casado, J.. "Alkylating potential of oxetanes". Chemical Research in Toxicology 23 7 (2010): 1275-1281. http://www.scopus.com/inward/record.url?eid=2-s2.0-77955359925&partnerID=MN8TOARS.
    10.1021/tx100153w
  27. Marohnic, C.C.; Panda, S.P.; McCammon, K.; Rueff, J.; Masters, B.S.S.; Kranendonk, M.. "Human cytochrome P450 oxidoreductase deficiency caused by the Y181D mutation: Molecular consequences and rescue of defect". Drug Metabolism and Disposition 38 2 (2010): 332-340. http://www.scopus.com/inward/record.url?eid=2-s2.0-76149123185&partnerID=MN8TOARS.
    10.1124/dmd.109.030445
  28. Palma, B.B.; Sousa, M.S.; Vosmeer, C.R.; Lastdrager, J.; Rueff, J.; Vermeulen, N.P.E.; Kranendonk, M.. "Functional characterization of eight human cytochrome P450 1A2 gene variants by recombinant protein expression". Pharmacogenomics Journal 10 6 (2010): 478-488. http://www.scopus.com/inward/record.url?eid=2-s2.0-78649330101&partnerID=MN8TOARS.
    10.1038/tpj.2010.2
  29. Franke-Fayard, B.; Djokovic, D.; Dooren, M.W.; Ramesar, J.; Waters, A.P.; Falade, M.O.; Kranendonk, M.; et al. "Simple and sensitive antimalarial drug screening in vitro and in vivo using transgenic luciferase expressing Plasmodium berghei parasites". International Journal for Parasitology 38 14 (2008): 1651-1662. http://www.scopus.com/inward/record.url?eid=2-s2.0-55549120197&partnerID=MN8TOARS.
    10.1016/j.ijpara.2008.05.012
  30. Kranendonk, M.; Marohnic, C.C.; Panda, S.P.; Duarte, M.P.; Oliveira, J.S.; Masters, B.S.S.; Rueff, J.. "Impairment of human CYP1A2-mediated xenobiotic metabolism by Antley-Bixler syndrome variants of cytochrome P450 oxidoreductase". Archives of Biochemistry and Biophysics 475 2 (2008): 93-99. http://www.scopus.com/inward/record.url?eid=2-s2.0-44949214648&partnerID=MN8TOARS.
    10.1016/j.abb.2008.04.014
  31. Duarte, M.P.; Palma, B.B.; Gilep, A.A.; Laires, A.; Oliveira, J.S.; Usanov, S.A.; Rueff, J.; Kranendonk, M.. "The stimulatory role of human cytochrome b5 in the bioactivation activities of human CYP1A2, 2A6 and 2E1: A new cell expression system to study cytochrome P450-mediated biotransformation (a corrigendum report on Duarte et al. (2005) Mutagenesis 20, 93-100)". Mutagenesis 22 1 (2007): 75-81. http://www.scopus.com/inward/record.url?eid=2-s2.0-33846094609&partnerID=MN8TOARS.
    10.1093/mutage/gel054
  32. Maralhas, A.; Monteiro, A.; Martins, C.; Kranendonk, M.; Laires, A.; Rueff, J.; Rodrigues, A.S.. "Genotoxicity and endoreduplication inducing activity of the food flavouring eugenol". Mutagenesis 21 3 (2006): 199-204. http://www.scopus.com/inward/record.url?eid=2-s2.0-33745113204&partnerID=MN8TOARS.
    10.1093/mutage/gel017
  33. Duarte, M.P.; Palma, B.B.; Gilep, A.A.; Laires, A.; Oliveira, J.S.; Usanov, S.A.; Rueff, J.; Kranendonk, M.. "The stimulatory role of human cytochrome b5 in the bioactivation activities of human CYP1A2, 2A6 and 2E1: A new cell expression system to study cytochrome P450 mediated biotransformation". Mutagenesis 20 2 (2005): 93-100. http://www.scopus.com/inward/record.url?eid=2-s2.0-18344393458&partnerID=MN8TOARS.
    10.1093/mutage/gei012
  34. Duarte, M.P.; Palma, B.B.; Laires, A.; Oliveira, J.S.; Rueff, J.; Kranendonk, M.. "Escherichia coli BTC, a human cytochrome P450 competent tester strain with a high sensitivity towards alkylating agents: Involvement of alkyltransferases in the repair of DNA damage induced by aromatic amines". Mutagenesis 20 3 (2005): 199-208. http://www.scopus.com/inward/record.url?eid=2-s2.0-19544372412&partnerID=MN8TOARS.
    10.1093/mutage/gei028
  35. Duarte, MP; Palma, BB; Laires, A; Oliveira, JS; Rueff, J; Kranendonk, M. "BTC, a human P450 containing Escherichia coli tester strain with a high sensitivity towards alkylating agents". Drug Metabolism Reviews 35 (2003): 127-127. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000182128400253&KeyUID=WOS:000182128400253.
  36. Rueff, J.; Gaspar, J.; Kranendonk, M.. "DNA polymorphisms as modulators of genotoxicity and cancer". Biological Chemistry 383 6 (2002): 923-932. http://www.scopus.com/inward/record.url?eid=2-s2.0-0036333019&partnerID=MN8TOARS.
    10.1515/BC.2002.099
  37. Kranendonk, M.; Laires, A.; Rueff, J.; Estabrook, R.W.; Vermeulen, N.P.E.. "Heterologous expression of xenobiotic mammalian-metabolizing enzymes in mutagenicity tester bacteria: An update and practical considerations". Critical Reviews in Toxicology 30 3 (2000): 287-306. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034080524&partnerID=MN8TOARS.
  38. Kranendonk, M.; Fisher, C.W.; Roda, R.; Carreira, F.; Theisen, P.; Laires, A.; Rueff, J.; Vermeulen, N.P.E.; Estabrook, R.W.. "Escherichia coli MTC, a NADPH cytochrome P450 reductase competent mutagenicity tester strain for the expression of human cytochrome P450: Comparison of three types of expression systems". Mutation Research - Genetic Toxicology and Environmental Mutagenesis 439 2 (1999): 287-300. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033006924&partnerID=MN8TOARS.
    10.1016/S1383-5718(98)00193-4
  39. Kranendonk, M.; Carreira, F.; Theisen, P.; Laires, A.; Fisher, C.W.; Rueff, J.; Estabrook, R.W.; Vermeulen, N.P.E.. "Escherichia coli MTC, a human NADPH P450 reductase competent mutagenicity tester strain for the expression of human cytochrome P450 isoforms 1A1, 1A2, 2A6, 3A4, or 3A5: Catalytic activities and mutagenicity studies". Mutation Research - Genetic Toxicology and Environmental Mutagenesis 441 1 (1999): 73-83. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032910448&partnerID=MN8TOARS.
    10.1016/S1383-5718(99)00032-7
  40. Kranendonk, M.; Mesquita, P.; Laires, A.; Vermeulen, N.P.E.; Rueff, J.. "Expression of human cytochrome P450 1A2 in Escherichia coli: A system for biotransformation and genotoxicity studies of chemical carcinogens". Mutagenesis 13 3 (1998): 263-269. http://www.scopus.com/inward/record.url?eid=2-s2.0-0031748441&partnerID=MN8TOARS.
    10.1093/mutage/13.3.263
  41. Kranendonk, M.; Commandeur, J.N.M.; Laires, A.; Rueff, J.; Vermeulen, N.P.E.. "Characterization of enzyme activities and cofactors involved in bioactivation and bioinactivation of chemical carcinogens in the tester strains Escherichia coli K12 MX100 and Salmonella typhimurium LT2 TA100". Mutagenesis 12 4 (1997): 245-254. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030747265&partnerID=MN8TOARS.
    10.1093/mutage/12.4.245
  42. Kranendonkl, M.; Mesquitai, P.; Laires, A.; Vermeulen, N.P.E.; Rueffi, J.. "Expression of human cytochrome P450 1A2 in esctierichia colh a new system for biotransformation and genotoxicity studies of chemical carcinogens". FASEB Journal 11 9 (1997): http://www.scopus.com/inward/record.url?eid=2-s2.0-0344892937&partnerID=MN8TOARS.
  43. Kranendonk, M.; Pintado, F.; Mesquita, P.; Laires, A.; Vermeulen, N.P.E.; Rueff, J.. "MX100, a new Escherichia coli tester strain for use in genotoxicity studies". Mutagenesis 11 4 (1996): 327-333. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030036804&partnerID=MN8TOARS.
    10.1093/mutage/11.4.327
  44. Kranendonk, M.; Ruas, M.; Laires, A.; Rueff, J.. "Isolation and prevalidation of an Escherichia coli tester strain for the use in mechanistic and metabolic studies of genotoxins". Mutation Research/Environmental Mutagenesis and Related Subjects 312 2 (1994): 99-109. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028082743&partnerID=MN8TOARS.
    10.1016/0165-1161(94)90014-0
  45. Rademaker, B.; Kramer, K.; Van Ingen, H.; Kranendonk, M.; Timmerman, H.. "Non-specific binding of the fluorescent b-adrenergic receptor probe alprenolol-NBD". Journal of Receptors and Signal Transduction 5 2-3 (1985): 121-131. http://www.scopus.com/inward/record.url?eid=2-s2.0-0022415408&partnerID=MN8TOARS.
    10.3109/10799898509041874
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2019/06/24 Putative motif in the FMN-domain of human cytochrome P450 oxidoreductase involved in its interaction with structurally diverse redox partners 21th International Conference on Cytochrome P450
The University of Queensland (Brisbane, Australia)
2019 Molecular Insights in the Interaction of Human Cytochrome P450 Oxidoreductase with its Structural Diverse Redox Partners: the Quest of Understanding Genetic Susceptibility IV Genetics Workshop NOVA-Health
Universidade NOVA de Lisboa (Lisbon, Portugal)
2018 Ionic interactions in the protein dynamics of Cytochrome P450 oxidoreductase National Science Summit – Ciência 2018
Fundação para a Ciência e Tecnologia (Lisbon, Portugal)
2017 Drug Metabolism is mediated by “sloppy” enzymes - the case of cytochrome P450 Seminar serie, Faculty of Pharmacy, Universidade de Lisboa
(Lisbon, Portugal)
2017 The hinge region of human NADPH-cytochrome P450 reductase in conformational switching: the critical role of ionic strength. 20th International Conference on Cytochrome P450
(Düsseldorf, Germany)
2017 Cytochrome P450 reductase chimera: turn around the hinge 19th International Symposium on Flavins and Flavoproteins
(Groningen, Netherlands)
2017 The hinge region of human NADPH-cytochrome P450 reductase in conformational switching: the critical role of ionic strength National Science Summit – Ciência 2017
Fundação para a Ciência e Tecnologia (Lisbon, Portugal)
2016 Cytochromes P450s and their role in Variable Drug Metabolism: An Introduction iMed8.0 Meeting, Workshop Genetics
NOVA Medical School, Universidade NOVA de Lisboa (Lisbon, Portugal)
2016 Drug Metabolism is mediated by “sloppy” enzymes - the case of cytochrome P450 Seminar serie, CEDOC – NOVA Medical School
NOVA Medical School | FCM, Universidade Nova de Lisboa (Lisbon, Portugal)
2016 Instability of the Human Cytochrome P450 Reductase A287P Variant Is the Major Contributor to the Antley-Bixler Syndrome-Like Phenotype 2nd Genetics Workshop NOVA Health, Universidade NOVA Lisboa
Universidade NOVA de Lisboa (Lisbon, Portugal)
2013 New and “old” in vitro tools for the study of polymorphic variants of the human cytochrome P450 enzyme complex 2nd Conference of the European Society of Pharmacogenomics and Personalized Therapy
INFARMED (Lisbon, Portugal)
2013 Pharmacokinetic/toxicokinetic studies in Pharmacogenetics: Principles and requisites of in vitro models exemplified with human POR and CYP1A2 polymorphism studies Annual Conference of the Portuguese Pharmacology Society
(Porto, Portugal)
2011 Altered human CYP3A4 activity caused by Antley Bixler Syndrome-related variants of NADPH-cytochrome P450 oxidoreductase measured in a robust in vitro system 17th International Conference on Cytochromes P450
(Manchester, United Kingdom)
2007 Antley-Bixler Syndrome-Related Mutants of Human NADPH-Cytochrome P450 Reductase: Effects on Drug Metabolism and Mutagenesis 15th International Conference on Cytochromes P450
(Bled, Slovenia)
2005 Human cytochrome P450 competent Bacterial Cell-systems. Applications in Genotoxicology
University of Texas Health Science Center at San Antonio (San Antonio, United States)
1999 Development of specialized mutagenicity tester bacteria for the mechanistic study of xenobiotics
Texas Tech University, School of Pharmacy (Amarillo TX, United States)
1999 Eschericia coli MTC, a human NADPH cytochrome P450 reductase competent mutagenicity tester strain for the expression of human cytochrome P450s 9th North American ISSX Meeting
(Nashville, United States)
1998 Development of human cytochrome P450 competent mutagenicity tester bacteria
Texas Tech University, School of Pharmacy (Amarillo, TX, United States)

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2020/11/01 - Current Toxicological profile of cellulose nanomaterials in human gastrointestinal cells
Co-supervisor
 Biomedicina (PhD)
Universidade Nova de Lisboa Faculdade de Ciências Médicas, Portugal
2020/09/01 - Current The Role of Progesterone Receptor Membrane Component 1 (PGRMC1) in Cytochromes P450 (CYP)-Mediated Drug Metabolism and its Implication in Chemotherapy Resistance
Supervisor
 Bioquímica para a Saúde (Master)
Universidade Nova de Lisboa Faculdade de Ciências Médicas, Portugal
2018 - 2019 O papel da Progesterone Receptor Membrane Component 1 no metabolismo de fármacos mediado por citocromo P450
Co-supervisor
 Bioquímica (Master)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2015 - 2019 Domain Dynamics and Control of Electron flux of NADPH cytochrome P450 oxidoreductase
Supervisor
 Ciências Biomédicas (PhD)
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2006 - 2017 Development of human cytochrome P450 competent genotoxicity tester bacterial systems for high throughput screening
Supervisor
Vrije Universiteit Amsterdam Faculteit der Exacte Wetenschappen, Netherlands
2013 - 2014 Sistemas bacterianos competentes em biotransformação humana para estudos mecanísticos e toxicológicos
Supervisor
 Genética Molecular e Biomedicina (Master)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2011 - 2012 Sistemas Bacterianos no Estudo da Bioactivação do Antirectoviral Nevirapina
Supervisor
 Genética Molecular e Biomedicina (Master)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2009 - 2010 O citocromo b5 no complexo enzimático de citocromo P450
Supervisor
 Genética Molecular e Biomedicina (Master)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2008 - 2009 Sistemas bacterianos do complexo enzimático de citocromos P450 humanos: Contribuição para a avaliação do mutante Q153R de NADPH oxidoreductase dos citocromos P450 e no desenvolvimento de de uma metodologia para o doseamento da NADPH oxidoreductase dos citocromos P450 utilizando células inteiras
Supervisor
 Ciências Biomédicas (Master)
Universidade do Algarve Faculdade de Ciências e Tecnologia, Portugal
2007 - 2008 Estudos de variantes polimórficas de citocromo P450 1A2 humano
Supervisor
 Biologia Celular e Biotecnologia (Master)
Universidade de Lisboa Faculdade de Ciências, Portugal
2000 - 2008 Desenvolvimento de Novos Sistemas de Escherichia coli Competentes em Biotransformação Humana: Aplicação no Estudo da Metabolização de Xenobióticos Mediada Pelos Citocromos P450
Co-supervisor
 Ciências do Ambiente (PhD)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2005 - 2006 Contribuição para o desenvolvimento de novas estirpes de Escherichia coli para estudos de biotransformação de xenobióticos
Supervisor
 Engenharia Biotecnológica (Degree)
Universidade Lusófona de Humanidades e Tecnologias, Portugal
2005 - 2006 DEVELOPMENT OF A NEW TECHNIQUE FOR HIGH-THROUGHPUT ANTIMALARIAL DRUG EFFICACY SCREENING IN VIVO
Co-supervisor
 Parasitologia Médica (Master)
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2002 - 2003 Desenvolvimento de novas estirpes de bácterias para estudos de biotransformação de xeobióticos - Coexpressão de citocromo b5 com citocromo P450 e NADPH-citocromo P450 reductase humanos, para testes de mutagenicidade bacterianos
Supervisor
 Bioquímica (Master)
Universidade de Lisboa Faculdade de Ciências, Portugal
2000 - 2001 Estudos de Toxicidade in vivo e in vitro de plantas medicinais antimaláricas das ilhas de São Tomé e Príncipe
Co-supervisor
 Química Aplicada (Master)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2000 - 2001 Desenvolvimento e pré-caracterização de novas estirpes de Escherichia coli com coexpressaõ simultânea de múltiplas formas de citocromos P450 humanos
Supervisor
 Bioquímica (Master)
Universidade de Lisboa Faculdade de Ciências, Portugal
1998 - 1999 Desenvolvimento de novas estirpes de Escherichia coli metabolicamente competentes a usar em testes de mutagenicidade
Supervisor
 Biologia Microbiana e Genética (Master)
Universidade de Lisboa Faculdade de Ciências, Portugal
1998 - 1999 Desenvolvimento de estirpes de Escherichia coli metabolicamente competentes para testes de mutagenicidade - Expressão heteróloga de citocromos P450 humanos
Supervisor
 Biologia Molecular Humana (Master)
Universidade Nova de Lisboa Faculdade de Ciências Médicas, Portugal
1995 - 1996 Desenvolvimento de novas estirpes de Escherichia coli para o estudo de mutagenicidade oxidativa
Supervisor
 Bioquímica (Master)
Universidade de Lisboa Faculdade de Ciências, Portugal
1993 - 1994 Melhoramento de uma estirpe de Escherichia coli a usar em testes de mutagenicidade - Determinação da sensibilidade a produtos oxidativos
Supervisor
 Química Aplicada (Master)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
1993 - 1994 Contribuiçaõ para o estudo de mecanismos de genotoxicidade da quercetina e de outros flavanoids
Co-supervisor
 Bioquímica (Master)
Universidade de Lisboa Faculdade de Ciências, Portugal
1992 - 1993 Isolamento de uma estirpe de Escherichia coli a usar em testes de mutagenicidade - Validação preliminar
Supervisor
 Bioquímica (Master)
Universidade de Lisboa Faculdade de Ciências, Portugal

Jury of academic degree

Topic
Role 		Candidate name (Type of degree)
Institution / Organization
2022/02/07 Role of Micro RNAs in Cancer Drug Resistence
President of the jury
 Ana Beatriz Vigário Domingues (Master)
Universidade Nova de Lisboa Faculdade de Ciências Médicas, Portugal
2021/04/15 Assessment of microRNAs as biomarkers of Disease Progression in Amyotrophic Lateral Sclerosis
President of the jury
 Ana Rita Gomes Pisco (Master)
Universidade Nova de Lisboa Faculdade de Ciências Médicas, Portugal
2021/02/03 Cellular Mechanisms of Toxicity of Ingested Nanomaterials
(Thesis) Main arguer
 Adriana Isabel Ramos Vieira (Master)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2019/05/28 Domain Dynamics and Control of Electron flux of NADPH cytochrome P450 oxidoreductase
(Thesis) Arguer
 Diana Isabel Viana da Fonseca Campelo (PhD)
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2017/10/16 Development of human cytochrome P450 competent genotoxicity tester bacterial systems for high throughput screening
(Thesis) Arguer
 Bernardo José Espadinha de Brito Palma (PhD)
Vrije Universiteit Amsterdam Faculteit der Exacte Wetenschappen, Netherlands
2017/01/31 Study of the Natural Alkenylbenzenes Compounds: Mechanisms of DNA Lesions and Implications for Human Health
(Thesis) Arguer
 Célia Maria da Silva Martins (PhD)
Universidade Nova de Lisboa Faculdade de Ciências Médicas, Portugal
2015 Sistemas bacterianos competentes em biotransformação humana para estudos mecanísticos e toxicológicos
(Thesis) Arguer
 Pedro Miguel da Silva Antunes (Master)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2013 Sistemas Bacterianos no Estudo da Bioactivação do Antirectoviral Nevirapina
(Thesis) Arguer
 Mónica Filipa Neves Moço Alves (Master)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2011/12/12 Chemical processes that can damage cellular DNA: Reactivity and alkylating potential of some O-heterocycles
(Thesis) Arguer
 Rafael Gómez Bombarelli (PhD)
Universidad de Salamanca Facultad de Ciencias Químicas, Spain
2011/02 Early Detection of the Biological and Genetic Determinants of Resistance to Artemisin-Based Combination Therapy in Malaria Parasites
(Thesis) Arguer
 Louise Alves Pereira Rodrigues (PhD)
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2011 O citocromo b5 no complexo enzimático do citocromo P450
(Thesis) Arguer
 Daniela Figueiredo Simões Presa (Master)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2010 Sistemas bacterianos do complexo enzimático de citocromos P450 humanos: Contribuição para a avaliação do mutante Q153R de NADPH oxidoreductase dos citocromos P450 e no desenvolvimento de de uma metodologia para o doseamento da NADPH oxidoreductase dos citocromos P450 utilizando células inteiras
(Thesis) Main arguer
 Carmen Luísa Silva Lourenço (Master)
Universidade do Algarve Faculdade de Ciências e Tecnologia, Portugal
2008/07/02 Desenvolvimento de Novos Sistemas de Escherichia coli Competentes em Biotransformação Humana: Aplicação no Estudo da Metabolização de Xenobióticos Mediada Pelos Citocromos P450
(Thesis) Arguer
 Maria Paula Amaro de Castilho Duarte (PhD)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2008 Estudos de variantes polimórficas de citocromo P450 1A2 humano
(Thesis) Arguer
 Marta Sofia Taborda Silva e Sousa (Master)
Universidade de Lisboa Faculdade de Ciências, Portugal
2007/03/21 Towards Novel Biocatalysts for Drugs and Drug-like Molecules
(Thesis) Arguer
 Barbara Medea Alice van Vught-Lussenburg (PhD)
Vrije Universiteit Amsterdam Faculteit der Exacte Wetenschappen, Netherlands
2003 Desenvolvimento de Novas Estirpes de Bactérias para Estudos de Biotransformação de Xenobióticos
(Thesis) Arguer
 Bernardo José Espadinha de Brito Palma (Master)
Universidade de Lisboa Faculdade de Ciências, Portugal

Committee member

Activity description
Role 		Institution / Organization
2019/10/23 - Current Member of the Internal Advisory Committee of the International Conferences on Cytochrome P450 series. (URL: https://my.vanderbilt.edu/p450meetings/iac/)
Member
 Vanderbilt University School of Medicine, United States
2018/10 - Current Management Commmittee member of the European Cost Action CA17112 Prospective European Drug Induced Liver Injury Network
Member
 European Cooperation in Science and Technology, Belgium
2018/10 - Current Core Committee member of the the European COST Action CA17112 Prospective European Drug Induced Liver Injury Network
Member
 European Cooperation in Science and Technology, Belgium
2015/01/01 - Current Member of the Coordination Board of the Center for Toxicogenomics and Health (ToxOmics),
Member
 Universidade Nova de Lisboa, Portugal
2010 - 2020 Member of the Scientific Council - NOVA Medical School
Member
 Universidade Nova de Lisboa Faculdade de Ciências Médicas, Portugal

Consulting

Activity description Institution / Organization
2019 - Current Training course Introduction to Toxicology ASCENZA AGRO, Portugal
2013 - 2013 Training course Ames mutagenicity test ASCENZA AGRO, Portugal
1994 - 1995 Liaison Officer Rephartox BV, Netherlands
1988 - 1989 Consultant Dalton BV, Netherlands

Course / Discipline taught

Academic session Degree Subject (Type) Institution / Organization
2020/05 - Current Curso Pós-graduado em Genética e Genómica na Clínica Genética e Genómica na Clínica (Pós-Graduação) Universidade Nova de Lisboa Faculdade de Ciências Médicas, Portugal
2018 - Current Lic. Nutrition Sciences - Lecturer of Genetics Module Ciências da Nutrição (Licenciatura) Universidade Nova de Lisboa Faculdade de Ciências Médicas, Portugal
2017 - Current MSc Integrated Masters in Medicine - Lecturer in Human Toxicology and Genetics Medicina (Mestrado integrado) Universidade Nova de Lisboa Faculdade de Ciências Médicas, Portugal
2017 - Current MSc NOVA Biomedical Research - Lecturer of Advanced Research Methods module Investigação Biomédica (Mestrado integrado) Universidade Nova de Lisboa Faculdade de Ciências Médicas, Portugal
2014 - Current MSc Oncobiology – Molecular Mechanisms in Cancer - Lecturer of the Genetic Toxicology module ONCOBIOLOGIA - MECANISMOS MOLECULARES DO CANCRO (Mestrado) Universidade do Algarve Departamento de Ciências Biomédicas e Medicina, Portugal
2013 - Current MSc Biochemistry in Health - Lecturer of the Principles of Toxicology module Bioquímica para a Saúde (Mestrado) Universidade Nova de Lisboa Faculdade de Ciências Médicas, Portugal
2009 - Current PhD Medicine - Lecturer of the Advanced Genetics Module Medicina (Doutoramento) Universidade Nova de Lisboa Faculdade de Ciências Médicas, Portugal
2007 - Current MSc Integrated Masters in Medicine - Lecturer in Genetics Medicina (Mestrado integrado) Universidade Nova de Lisboa Faculdade de Ciências Médicas, Portugal
2013 - 2018 PhD Human Genetics and Infectious Disease - Lecture of the Human Genetics, Computational Biology and Bioinformatics, Laboratory in vivo and in vitro Experimentation, and Molecular Toxicology modules Genética Humana e Doenças Infeciosas (Doutoramento) Universidade Nova de Lisboa Faculdade de Ciências Médicas, Portugal
2009/04 - 2009/07 Academic Extension Course Genetics and Genomics in Toxicology - Coordinator of Module 4: Biotransformation in Genotoxicology: Cytochrome P450 CURSO DE EXTENSÃO UNIVERSITÁRIA EM TOXICOLOGIA GENÉTICA E TOXICOGENÓMICA (Especialização pós-licenciatura) Universidade Nova de Lisboa - Centro de Investigação em Genética Molecular Humana, Portugal
2006/04 - 2006/06 Academic Extension Course Genetics and Genomics in Toxicology - Coordinator of Module 3: Biotransformation in Genotoxicology: Cytochrome P450 CURSO DE EXTENSÃO UNIVERSITÁRIA EM TOXICOLOGIA GENÉTICA E TOXICOGENÓMICA (Especialização pós-licenciatura) Universidade Nova de Lisboa - Centro de Investigação em Genética Molecular Humana, Portugal

Evaluation committee

Activity description
Role 		Institution / Organization Funding entity
2014 - Current Evaluator of PhD Project-proposals and Progress reports of the three Doctoral Programs of the NOVA Medical School| Faculdade de Ciências Médicas
Evaluator
 Universidade Nova de Lisboa Faculdade de Ciências Médicas, Portugal
2017/01 - 2017/12 Jury member of the selection committee of the Internal admission tender to occupy a job in the category of auxiliary researcher - scientific area of chronic-degenerative and genetic diseases, specifically the area of human genetics, of the scientific research career, in the Department of Human Genetics - INSA
Member
 Instituto Nacional de Saúde Doutor Ricardo Jorge, Portugal

Journal scientific committee

Journal title (ISSN) Publisher
2021/03/18 - Current Frontiers in Toxicogenomics Frontiers Media SA, part of Nature Publishing Group
2013 - Current Frontiers in Pharmacology/Pharmacogenetics and Pharmacogenomics (1663-9812) Frontiers Media SA, part of Nature Publishing Group
2019 - 2020 Human Reproduction (1460-2350) Oxford University Press
2019 - 2019 Biomolecules (2218-273X) MDPI AG
2019 - 2019 Scientific Reports (2045-2322) Springer Science and Business Media LLC
2018 - 2019 FEBS Journal (1742-4658) Wiley (Blackwell Publishing)
2010 - 2018 Xenobiotica (1366-5928) Informa UK (Taylor & Francis)
2006 - 2018 Mutation Research/Genetic Toxicology and Environmental Mutagenesis (1383-5718) Elsevier
2016 - 2016 Fundamental and Clinical Pharmacology (1472-8206) Wiley (Blackwell Publishing)
2016 - 2016 Pharmacogenetics and Genomics (1744-6872) Ovid Technologies (Wolters Kluwer) - Lippincott Williams & Wilkins
2009 - 2016 Toxicology in Vitro (0887-2333) Elsevier
2015 - 2015 Biochimica et Biophysica Acta (BBA) - General Subjects (0304-4165) Elsevier
2013 - 2015 Drug Metabolism and Disposition (1521-009X) American Society for Pharmacology & Experimental Therapeutics
2013 - 2013 Archives in Toxicology ( ¿0340-5761) Springer
2010 - 2011 The Open Drug Metabolism Journal (1874-0731) Bentham Science
2009 - 2010 Drug Metabolism Letters (1872-3128) Bentham Science
2009 - 2010 Toxicology Letters (0378-4274) Elsevier
2007 - 2007 Food and Chemical Toxicology (0278-6915) Elsevier
2006 - 2006 Basic & Clinical Pharmacology & Toxicology (1742-7843) Wiley (Blackwell Publishing)
2005 - 2005 International Journal of Cancer (1097-0215) Wiley (John Wiley & Sons)
2001 - 2004 Environmental Toxicology and Pharmacology (1382-6689) Elsevier

Mentoring / Tutoring

Topic Student name
2019 - Current Mentoring Assistant Researcher Doutor Francisco Esteves
2016/01 - Current Protein dynamics of the human NADPH cytochrome P450 reductase Francisco Vaz de Carvalho Esteves
2014/09 - 2019/05 Domain dynamics and control of electron flux of NADPH cytochrome P450 oxidoreductase Diana Isabel Viana da Fonseca Campelo Delgado
2017/09 - 2018/02 Protein dynamics of the human NADPH cytochrome P450 reductase Bernardo José Espadinha de Brito Palma
2015 - 2018 Mentoring postdoc team member Doutor Francisco Esteves
2017/09 - 2017/11 Protein dynamics of the human NADPH cytochrome P450 reductase Bruno Daniel da Costa Gomes
2012/03 - 2012/07 Mutagenic effect of nitrosated biologicals Mario González Jiménez - Universidad d´Salamanca
2008 - 2011 The study of Antley-Bixler Syndrome related mutations of cytochrome P450 oxidoreductase: CYPOR polymorphism and the human cytochrome P450 enzyme complex Daniela Mesquita Moutinho
2009/10 - 2010/05 Determination of the effects of human PGRMC1 on the activities of human CYP-mediated biotransformation Albertina Pires Lopes - Universidade de Aveiro
2009/08 - 2009/12 DNA alkylating potential of O-heterocyclic compounds Rafael Gómez Bombarelli - Universidad d' Salamanca
2004 - 2005 Structural and functional analysis of P450c17 involvement in steroidogenesis and xenobiotics metabolism Andrei Aleksandrovich Gilep - Institute of Bioorganic Chemistry, Minsk Belarus
2003/02 - 2003/03 Heterologous expression of human cytochrome P450s in Escherichia coli Barbara Medea Alice van Vught-Lussenburg - Vrije Universtiteit Amsterdam

Other jury / evaluation

Activity description Institution / Organization
2017/10 - 2017/11 Evaluator of the Activity Report 2014-2017 of the Assistant Researcher for definitive appointment of the Career Scientific Research from the National Institute of Health Dr. Ricardo Jorge Instituto Nacional de Saúde Doutor Ricardo Jorge, Portugal
2016 - 2016 Evaluator of first triennium - post-doc grants Fundação para a Ciência e a Tecnologia, Portugal
2012/01 - 2012 Evaluator of grant application to the Natural Sciences and Engineering Research Council of Canada Natural Sciences and Engineering Research Council of Canada, Canada
2009 - 2009 Evaluator of grant application to the Czech Science Foundation Czech Science Foundation - Grantová agentura Ceské republiky, Czech Republic
Distinctions

Award

2007 Best proposed project in the program “Promoção de Projectos de Investigação Clínica na Rede de Hospitais Articulados com a Faculdade de Ciências Médicas, Universidade Nova de Lisboa
Universidade Nova de Lisboa Faculdade de Ciências Médicas, Portugal