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Identification

Personal identification

Full name
Paul Alexandre De Sousa

Author identifiers

Ciência ID
FD1F-26BA-A715
ORCID iD
0000-0003-0745-2504
Outputs

Publications

Book
  1. Bruce, K.W.; Campbell, J.D.M.; De Sousa, P.. Quality Assured Characterization of Stem Cells for Safety in Banking for Clinical Application. 2017.
    10.1007/978-1-4939-6921-0_6
Book chapter
  1. Fraser, L.; Bruce, K.; Campbell, J.M.; De Sousa, P.A.. "Quality assessment and production of human cells for clinical use". 607-629. 2018.
    10.1007/978-1-4939-7825-0_28
  2. Hoeve, M.A.; De Sousa, P.A.; Willoughby, N.A.. "Challenges of Scale-up of Cell Separation and Purification Techniques". 127-165. 2016.
  3. De Sousa, P.A.; McRae, S.; Stacey, G.. "Stem cells in the development of products for regenerative medicine". 77-97. 2012.
Journal article
  1. Eirini Koutsouraki; Steve Pells; Paul A. De Sousa. "Sufficiency of hypoxia-inducible 2-oxoglutarate dioxygenases to block chemical oxidative stress-induced differentiation of human embryonic stem cells". Stem Cell Research 34 (2019): 101358-101358. https://doi.org/10.1016/j.scr.2018.11.019.
    10.1016/j.scr.2018.11.019
  2. De Sousa PA; Ritchie D; Green A; Chandran S; Knight R; Head MW. "Renewed assessment of the risk of emergent advanced cell therapies to transmit neuroproteinopathies.". Acta neuropathologica (2018): http://europepmc.org/abstract/med/30483944.
    10.1007/s00401-018-1941-9
  3. Paul A. De Sousa; Rachel Steeg; Beate Kreisel; Timothy E. Allsopp. "Hot Start to European Pluripotent Stem Cell Banking". Trends in Biotechnology 35 7 (2017): 573-576. https://doi.org/10.1016/j.tibtech.2017.04.006.
    10.1016/j.tibtech.2017.04.006
  4. Paul A. De Sousa; Rachel Steeg; Elisabeth Wachter; Kevin Bruce; Jason King; Marieke Hoeve; Shalinee Khadun; et al. "Rapid establishment of the European Bank for induced Pluripotent Stem Cells (EBiSC) - the Hot Start experience". Stem Cell Research 20 (2017): 105-114. https://doi.org/10.1016%2Fj.scr.2017.03.002.
    10.1016/j.scr.2017.03.002
  5. P.A. De Sousa; J.M. Downie; B.J. Tye; K. Bruce; P. Dand; S. Dhanjal; P. Serhal; et al. "Development and production of good manufacturing practice grade human embryonic stem cell lines as source material for clinical application". Stem Cell Research 17 2 (2016): 379-390. https://doi.org/10.1016%2Fj.scr.2016.08.011.
    10.1016/j.scr.2016.08.011
  6. Davis MR; Arner E; Duffy CR; De Sousa PA; Dahlman I; Arner P; Summers KM. "Datasets of genes coexpressed with FBN1 in mouse adipose tissue and during human adipogenesis.". Data in brief (2016): http://europepmc.org/abstract/med/27508231.
    10.1016/j.dib.2016.06.055
  7. Davis MR; Arner E; Duffy CR; De Sousa PA; Dahlman I; Arner P; Summers KM. "Expression of FBN1 during adipogenesis: Relevance to the lipodystrophy phenotype in Marfan syndrome and related conditions.". (2016): http://europepmc.org/abstract/med/27386756.
    10.1016/j.ymgme.2016.06.009
  8. P.A. De Sousa; B.J. Tye; K. Bruce; P. Dand; G. Russell; D.M. Collins; A. Greenshields; et al. "Derivation of the clinical grade human embryonic stem cell line RCe018-A (RC-14)". Stem Cell Research (2016): http://dx.doi.org/10.1016/j.scr.2016.04.004.
    10.1016/j.scr.2016.04.004
  9. "Derivation of the human embryonic stem cell line RCe008-A (RC-4)". Stem Cell Research (2016):
    10.1016/j.scr.2016.02.039
  10. De Sousa PA; Tye BJ; Bruce K; Dand P; Russell G; Collins DM; Greenshields A; et al. "Derivation of the clinical grade human embryonic stem cell line RCe019-A (RC-15).". (2016): http://europepmc.org/abstract/med/27346200.
    10.1016/j.scr.2016.04.003
  11. De Sousa PA; Tye BJ; Bruce K; Dand P; Russell G; Collins DM; Greenshields A; et al. "Derivation of the clinical grade human embryonic stem cell line RCe020-a (RC-16).". (2016): http://europepmc.org/abstract/med/27346205.
    10.1016/j.scr.2016.04.002
  12. De Sousa PA; Tye B; Bruce K; Dand P; Russell G; Gardner J; Downie JM; Bateman M; Courtney A. "Derivation of the human embryonic stem cell line RCe007-A (RC-3).". (2016): http://europepmc.org/abstract/med/27346192.
    10.1016/j.scr.2016.02.041
  13. De Sousa PA; Tye BJ; Bruce K; Dand P; Russell G; Collins DM; Greenshields A; et al. "Derivation of the clinical grade human embryonic stem cell line RCe016-A (RC-12).". (2016): http://europepmc.org/abstract/med/27346204.
    10.1016/j.scr.2016.04.001
  14. P.A. De Sousa; B.J. Tye; K. Bruce; P. Dand; G. Russell; D.M. Collins; A. Greenshields; et al. "Derivation of the clinical grade human embryonic stem cell line RCe017-A (RC-13)". Stem Cell Research (2016):
    10.1016/j.scr.2016.04.006
  15. P.A. De Sousa; B.J. Tye; K. Bruce; P. Dand; G. Russell; D.M. Collins; A. Greenshields; et al. "Derivation of the human embryonic stem cell line RCe014-A (RC-10)". Stem Cell Research 16 2 (2016): 537-540. https://doi.org/10.1016%2Fj.scr.2016.02.034.
    10.1016/j.scr.2016.02.034
  16. De Sousa PA; Tye BJ; Bruce K; Dand P; Russell G; Collins DM; Bradburn H; et al. "Derivation of the human embryonic stem cell line RCe010-A (RC-6).". (2016): http://europepmc.org/abstract/med/27346019.
    10.1016/j.scr.2016.02.035
  17. De Sousa PA; Tye BJ; Sneddon S; Bruce K; Dand P; Russell G; Collins DM; et al. "Derivation of the human embryonic stem cell line RCM1.". (2016): http://europepmc.org/abstract/med/27346018.
    10.1016/j.scr.2015.12.020
  18. De Sousa PA; Tye BJ; Bruce K; Dand P; Russell G; Collins DM; Greenshields A; et al. "Derivation of the human embryonic stem cell line RCe012-A (RC-8).". (2016): http://europepmc.org/abstract/med/27346021.
    10.1016/j.scr.2016.02.033
  19. De Sousa PA; Tye BJ; Collins DM; Bruce K; Dand P; Russell G; Bradburn H; et al. "Derivation of the human embryonic stem cell line RCe011-A (RC-7).". (2016): http://europepmc.org/abstract/med/27346020.
    10.1016/j.scr.2016.02.036
  20. De Sousa PA; Tye B; Bruce K; Dand P; Russell G; Gardner J; Downie JM; Bateman M; Courtney A. "Derivation of the human embryonic stem cell line RCe006-A (RC-2).". (2016): http://europepmc.org/abstract/med/27346014.
    10.1016/j.scr.2016.02.029
  21. De Sousa PA; Tye B; Bruce K; Dand P; Russell G; Collins DM; Gardner J; et al. "Derivation of the human embryonic stem cell line RCe009-A (RC-5).". (2016): http://europepmc.org/abstract/med/27346004.
    10.1016/j.scr.2016.02.030
  22. N. A. Willoughby; H. Bock; M. A. Hoeve; S. Pells; C. Williams; G. McPhee; P. Freile; D. Choudhury; P. A. De Sousa. "A scalable label-free approach to separate human pluripotent cells from differentiated derivatives". Biomicrofluidics 10 1 (2016): 014107-014107. https://doi.org/10.1063/1.4939946.
    10.1063/1.4939946
  23. De Sousa, P.A.; Tye, B.J.; Bruce, K.; Dand, P.; Russell, G.; Collins, D.M.; Greenshields, A.; et al. "Derivation of the clinical grade human embryonic stem cell line RCe015-A (RC-11)". Stem Cell Research 17 1 (2016): 42-48. http://www.scopus.com/inward/record.url?eid=2-s2.0-84969785238&partnerID=MN8TOARS.
    10.1016/j.scr.2016.04.021
  24. De Sousa, P.A.; Tye, B.J.; Bruce, K.; Dand, P.; Russell, G.; Collins, D.M.; Greenshields, A.; et al. "Derivation of the clinical grade human embryonic stem cell line RCe013-A (RC-9)". Stem Cell Research 17 1 (2016): 36-41. http://www.scopus.com/inward/record.url?eid=2-s2.0-84969916526&partnerID=MN8TOARS.
    10.1016/j.scr.2016.04.020
  25. De Sousa, P.A.; Tye, B.J.; Bruce, K.; Dand, P.; Russell, G.; Collins, D.M.; Greenshields, A.; et al. "Derivation of the clinical grade human embryonic stem cell line RCe021-A (RC-17)". Stem Cell Research 17 1 (2016): 1-5. http://www.scopus.com/inward/record.url?eid=2-s2.0-84966863452&partnerID=MN8TOARS.
    10.1016/j.scr.2016.04.019
  26. Mangani C; Lilienkampf A; Roy M; de Sousa PA; Bradley M. "Thermoresponsive hydrogel maintains the mouse embryonic stem cell "naive" pluripotency phenotype.". (2015): http://europepmc.org/abstract/med/26372076.
    10.1039/c5bm00121h
  27. Pells S; Koutsouraki E; Morfopoulou S; Valencia-Cadavid S; Tomlinson SR; Kalathur R; Futschik ME; De Sousa PA. "Novel Human Embryonic Stem Cell Regulators Identified by Conserved and Distinct CpG Island Methylation State.". (2015): http://europepmc.org/abstract/med/26151932.
    10.1371/journal.pone.0131102
  28. Canham MA; Van Deusen A; Brison DR; De Sousa PA; Downie J; Devito L; Hewitt ZA; et al. "The Molecular Karyotype of 25 Clinical-Grade Human Embryonic Stem Cell Lines.". (2015): http://europepmc.org/abstract/med/26607962.
    10.1038/srep17258
  29. Andrews PW; Baker D; Benvinisty N; Miranda B; Bruce K; Brüstle O; Choi M; et al. "Points to consider in the development of seed stocks of pluripotent stem cells for clinical applications: International Stem Cell Banking Initiative (ISCBI).". (2015): http://europepmc.org/abstract/med/25675265.
    10.2217/rme.14.93
  30. Tsenkina Y; Ruzov A; Gliddon C; Horsburgh K; De Sousa PA. "White matter tract and glial-associated changes in 5-hydroxymethylcytosine following chronic cerebral hypoperfusion.". (2014): http://europepmc.org/abstract/med/25305569.
    10.1016/j.brainres.2014.09.060
  31. Duffy CR; Zhang R; How SE; Lilienkampf A; De Sousa PA; Bradley M. "Long term mesenchymal stem cell culture on a defined synthetic substrate with enzyme free passaging.". (2014): http://europepmc.org/abstract/med/24780167.
    10.1016/j.biomaterials.2014.04.013
  32. Hansen A; Mjoseng HK; Zhang R; Kalloudis M; Koutsos V; de Sousa PA; Bradley M. "High-density polymer microarrays: identifying synthetic polymers that control human embryonic stem cell growth.". (2014): http://europepmc.org/abstract/med/24353271.
    10.1002/adhm.201300489
  33. Krejciova Z; De Sousa P; Manson J; Ironside JW; Head MW. "Human tonsil-derived follicular dendritic-like cells are refractory to human prion infection in vitro and traffic disease-associated prion protein to lysosomes.". (2014): http://europepmc.org/abstract/med/24183781.
    10.1016/j.ajpath.2013.09.013
  34. Yan X; Ehnert S; Culmes M; Bachmann A; Seeliger C; Schyschka L; Wang Z; et al. "5-azacytidine improves the osteogenic differentiation potential of aged human adipose-derived mesenchymal stem cells by DNA demethylation.". (2014): http://europepmc.org/abstract/med/24603866.
    10.1371/journal.pone.0090846
  35. Duffy, C.R.E.; Zhang, R.; How, S.-E.; Lilienkampf, A.; Tourniaire, G.; Hu, W.; West, C.C.; De Sousa, P.; Bradley, M.. "A high-throughput polymer microarray approach for identifying defined substrates for mesenchymal stem cells". Biomaterials Science 2 11 (2014): 1683-1692. http://www.scopus.com/inward/record.url?eid=2-s2.0-84907529682&partnerID=MN8TOARS.
    10.1039/c4bm00112e
  36. Krause KH; van Thriel C; De Sousa PA; Leist M; Hengstler JG. "Monocrotophos in Gandaman village: India school lunch deaths and need for improved toxicity testing.". (2013): http://europepmc.org/abstract/med/23943209.
    10.1007/s00204-013-1113-6
  37. De Sousa PA. "Biologically equivalent substitutive technology: what is needed to manufacture pluripotent stem cells for next-generation platforms for discovery and therapy.". (2013): http://europepmc.org/abstract/med/23998744.
    10.2217/rme.13.50
  38. Armit, Chris; Bauer, Nina G.; Besseling, Rut; Bradley, Mark; Duffy, Cairnan R. E.; Edenhofer, Frank; Helfen, Martina; et al. "A thermoresponsive and chemically defined hydrogel for long-term culture of human embryonic stem cells". Nature Communications 4 (2013):
    10.1038/ncomms2341
  39. De Sousa, Paul; Duffy, Cairnan R. E.; Mjoseng, Heidi K.; Pells, Steve; Pethig, Ronald; Smith, Stewart; Velugotla, Srinivas. "Dielectrophoresis based discrimination of human embryonic stem cells from differentiating derivatives". Biomicrofluidics 6 4 (2012):
    10.1063/1.4771316
  40. Bagnaninchi, Pierre; Corradetti, Bruna; Fahmy, Tarek M.; Freile, Paz; Park, Jason; Pells, Steve; de Sousa, Paul A.. "Paracrine signalling events in embryonic stem cell renewal mediated by affinity targeted nanoparticles". Biomaterials 33 28 (2012): 6634-6643.
    10.1016/j.biomaterials.2012.06.011
  41. Almeida, Rimple D.; De Sousa, Paul A.; Johnson, Andrew D.; Loose, Matthew; Ruzov, Alexey; Sottile, Virginie. "Semi-quantitative immunohistochemical detection of 5-hydroxymethyl-cytosine reveals conservation of its tissue distribution between amphibians and mammals". Epigenetics 7 2 (2012): 137-140.
    10.4161/epi.7.2.18949
  42. Bradley, Mark; De Sousa, Paul A.; Ermakov, Alexander; Freile, Paz; Ganeva, Veronika V.; Millar, Robert; Pawson, Adam; Pells, Steve; Wildenhain, Jan. "A role for intracellular calcium downstream of G-protein signaling in undifferentiated human embryonic stem cell culture". Stem Cell Research 9 3 (2012): 171-184.
    10.1016/j.scr.2012.06.007
  43. Courtney A; de Sousa P; George C; Laurie G; Tait J. "Balancing open source stem cell science with commercialization.". (2011): http://europepmc.org/abstract/med/21301432.
    10.1038/nbt.1773
  44. Saft, C.; Epplen; Wieczorek, S.; Landwehrmeyer, G.B.; Roos, R.A.C.; de Yebenes, J.G.; Dose, M.; et al. "NMDA receptor gene variations as modifiers in Huntington disease: A replication study". PLoS Currents (2011): http://www.scopus.com/inward/record.url?eid=2-s2.0-83655189292&partnerID=MN8TOARS.
    10.1371/currents.RRN1247
  45. Adya, Ashok K.; Bock, Henry; Canetta, Elisabetta; De Sousa, Paul; Kiss, Robert; Moore, Andrew J.; Pells, Steve; Willoughby, Nicholas A.. "Elasticity of Human Embryonic Stem Cells as Determined by Atomic Force Microscopy". Journal of Biomechanical Engineering-Transactions of the Asme 133 10 (2011):
    10.1115/1.4005286
  46. Barclay, G. Robin; Bishop, Matthew; Cancellotti, Enrico; De Sousa, Paul; Freile, Paz; Head, Mark W.; Ironside, James W.; et al. "Human embryonic stem cells rapidly take up and then clear exogenous human and animal prions in vitro". Journal of Pathology 223 5 (2011): 635-645.
    10.1002/path.2832
  47. Bai, Yu; Bradley, Mark; Chandran, Siddharthan; Chebotareva, Tatiana; De Sousa, Paul; Dudnakova, Tatiana; Fletcher, Judy; et al. "Lineage-specific distribution of high levels of genomic 5-hydroxymethylcytosine in mammalian development". Cell Research 21 9 (2011): 1332-1342.
    10.1038/cr.2011.113
  48. Amps, Katherine; Andrews, Peter W.; Anyfantis, George; Armstrong, Lyle; Avery, Stuart; Baharvand, Hossein; Baker, Duncan; et al. "Screening ethnically diverse human embryonic stem cells identifies a chromosome 20 minimal amplicon conferring growth advantage". Nature Biotechnology 29 12 (2011): 1132-U113.
    10.1038/nbt.2051
  49. Anderson, Richard A.; Bayne, Rosemary A. L.; De Sousa, Paul A.; Gardner, John. "Brain-derived neurotrophic factor is a regulator of human oocyte maturation and early embryo development". Fertility and Sterility 93 5 (2010): 1394-1406.
    10.1016/j.fertnstert.2009.04.007
  50. De Sousa, Paul; Menachery, Anoop; Pells, Steve; Pethig, Ronald. "Dielectrophoresis: A Review of Applications for Stem Cell Research". Journal of Biomedicine and Biotechnology (2010):
    10.1155/2010/182581
  51. Abbruzzese, Giovanni; Aguilar Barbera, Miquel; Albanese, Alberto; Andrews, Thomasin; Andrich, Jurgen; Antczak, Jakub; Arran, Natalie; et al. "Observing Huntington's Disease: the European Huntington's Disease Network's REGISTRY.". PLoS currents (2010):
    10.1371/currents.RRN1184
  52. Jordanides, Niove E.; Mountford, Joanne C.; Oliver, Emmanuel; Turner, Marc L.; de Sousa, Paul. "Red blood cells from pluripotent stem cells for use in transfusion". Regenerative Medicine 5 3 (2010): 411-423.
    10.2217/rme.10.22
  53. Orth M; Handley OJ; Schwenke C; Dunnett SB; Craufurd D; Ho AK; Wild E; et al. "Observing Huntington's Disease: the European Huntington's Disease Network's REGISTRY.". (2010): http://europepmc.org/abstract/med/20890398.
  54. Aziz NA; Jurgens CK; Landwehrmeyer GB; EHDN Registry Study Group; van Roon-Mom WM; van Ommen GJ; Stijnen T; Roos RA. "Normal and mutant HTT interact to affect clinical severity and progression in Huntington disease.". (2009): http://europepmc.org/abstract/med/19776381.
    10.1212/WNL.0b013e3181bd1121
  55. Brison, Daniel R.; Collins, Daniel M.; Cooke, Michael; Dand, Pawlina; De Sousa, Paul A.; Gardner, John; Kimber, Susan J.; et al. "Clinically failed eggs as a source of normal human embryo stem cells". Stem Cell Research 2 3 (2009): 188-197.
    10.1016/j.scr.2009.01.002
  56. Ahrlund-Richter, Lars; Alvarez, Manuel; Andrews, Peter W.; Arias-Diaz, Javard; Auerbach, Jonathan; Baker, Duncan; Ben-Josef, Dalit; et al. "Consensus Guidance for Banking and Supply of Human Embryonic Stem Cell Lines for Research Purposes". Stem Cell Reviews and Reports 5 4 (2009): 301-314.
    10.1007/s12015-009-9085-x
  57. Anderson, R. A.; Bayne, R. A. L.; Kinnell, H.; Pickering, S.; Sciorio, R.; Thong, K. J.; de Sousa, P. A.. "Cumulus gene expression as a predictor of human oocyte fertilisation, embryo development and competence to establish a pregnancy". Reproduction 138 4 (2009): 629-637.
    10.1530/REP-09-0144
  58. De Sousa, Paul A.; Wilmut, Ian. "Human parthenogenetic embryo stem cells: Appreciating what you have when you have it". Cell Stem Cell 1 3 (2007): 243-244.
    10.1016/j.stem.2007.08.006
  59. De Sousa, P.. "New Center to deliver GMP-grade stem cells". European Biotechnology Science and Industry News 5 11-12 (2006): 27-27. http://www.scopus.com/inward/record.url?eid=2-s2.0-33845576831&partnerID=MN8TOARS.
  60. King T; De Sousa PA. "Maintenance of pregnancy in pigs with limited viable embryos.". (2006): http://europepmc.org/abstract/med/16988373.
    10.1007/978-1-59745-154-3_5
  61. De Sousa, PA. "Optimising the therapeutic safety of human embryonic stem cells through the evolution of defined culture systems supporting their isolation". Expert Opinion on Biological Therapy 6 6 (2006): 551-554.
    10.1517/14712598.6.6.551
  62. De Sousa, Paul A.; Galea, George; Turner, Marc. "The road to providing human embryo stem cells for therapeutic use: the UK experience". Reproduction 132 5 (2006): 681-689.
    10.1530/rep.1.01080
  63. Brownstein, David G.; De Sousa, Paul A.; Delhanty, Joy; Dhanjal, Seema; Ferrier, Patricia M.; Fletcher, Judy M.; Gardner, John O.; et al. "Variations in humanized and defined culture conditions supporting derivation of new human embryonic stem cell lines". Cloning and Stem Cells 8 4 (2006): 319-334.
    10.1089/clo.2006.8.319
  64. Martins da Silva SJ; Gardner JO; Taylor JE; Springbett A; De Sousa PA; Anderson RA. "Brain-derived neurotrophic factor promotes bovine oocyte cytoplasmic competence for embryo development.". (2005): http://europepmc.org/abstract/med/15798017.
    10.1530/rep.1.00471
  65. Anderson, RA; Da Silva, SJM; De Sousa, PA. "Neurotrophin signaling in oocyte survival and developmental competence: A paradigm for cellular toti-potency". Cloning and Stem Cells 6 4 (2004): 375-385.
    10.1089/clo.2004.6.375
  66. De Sousa, P; Ferrier, T; Fletcher, J; Gao, SR; Gasparrini, B; Harkness, L; McGarry, M; et al. "Effect of cell confluence on production of cloned mice using an inbred embryonic stem cell line". Biology of Reproduction 68 2 (2003): 595-603.
    10.1095/biolreprod.102.005819
  67. De Sousa, P; Ferrier, T; Fletcher, J; Gao, SR; Gasparrini, B; Harkness, L; McGarry, M; et al. "Effects of donor oocytes and cultural conditions on development of cloned mice embryos". Molecular Reproduction and Development 66 2 (2003): 126-133.
    10.1002/mrd.10300
  68. De Sousa, PA; King, TJ; McGarry, M; Rhind, SM; Taylor, JE; Wilmut, I. "Human cloning: Can it be made safe?". Nature Reviews Genetics 4 11 (2003): 855-864.
    10.1038/nrg1205
  69. Aslam, S; Burl, S; Clark, AJ; Cui, W; Denning, C; Ferrier, P; Ross, A; et al. "Proliferative lifespan is conserved after nuclear transfer". Nature Cell Biology 5 6 (2003): 535-538.
    10.1038/ncb992
  70. Ainslie, A; De Sousa, PA; Fletcher, J; Gao, S; Gasparrini, B; McGarry, M; Overstrom, EW; et al. "Cloned mice derived from embryonic stem cell karyoplasts and activated cytoplasts prepared by induced enucleation". Biology of Reproduction 68 4 (2003): 1259-1266.
    10.1095/biolreprod.102.008730
  71. Beaujean, N; Dinnyes, A; King, TJ; Paterson, LA; Wells, DN; Wilmut, I; Young, LE; de Sousa, PA. "Somatic cell nuclear transfer". Nature 419 6907 (2002): 583-586.
    10.1038/nature01079
  72. Ainslie, A; Albertini, D; Archibald, AL; Bosma, W; Bowering, J; Bracken, J; De Sousa, PA; et al. "Somatic cell nuclear transfer in the pig: Control of pronuclear formation and integration with improved methods for activation and maintenance of pregnancy". Biology of Reproduction 66 3 (2002): 642-650.
    10.1095/biolreprod66.3.642
  73. De Sousa, Paul; Dinnyes, Andras; King, Tim; Wilmut, Ian. "Somatic cell nuclear transfer: Recent progress and challenges". Cloning and Stem Cells 4 1 (2002): 81-90.
    10.1089/153623002753632075
  74. Bosma, W; Day, BN; De Sousa, PA; Dinnyes, A; Dobrinsky, JR; Finlayson, HA; Harkness, L; et al. "Embryo development and establishment of pregnancy after embryo transfer in pigs: coping with limitations in the availability of viable embryos". Reproduction 123 4 (2002): 507-515.
    10.1530/rep.0.1230507
  75. De Sousa, P. A.; King, T. J.; Wilmut, I.; Zhu, J.. "Evaluation of pregnancy maintenance regimes in the pig for assisted reproductive technologies". Research in Veterinary Science 72 Supplement (2002): 4-4.
    10.1016/S0034-5288(02)90013-5
  76. De Sousa, P; Ferrier, T; Fletcher, J; Gao, SR; Gasparrini, B; Harkness, L; McGarry, M; Wilmut, I. "Germinal vesicle material is essential for nucleus remodeling after nuclear transfer". Biology of Reproduction 67 3 (2002): 928-934.
    10.1095/biolreprod.102.004606
  77. De Sousa, PA; Dobrinsky, JR; Fletcher, J; Springbett, A; Telfer, EE; Wilmut, I; Zhu, J. "Improvement of an electrical activation protocol for porcine oocytes". Biology of Reproduction 66 3 (2002): 635-641.
    10.1095/biolreprod66.3.635
  78. Ainslie, A; Bracken, J; Burl, S; Clark, AJ; Denning, C; Dinnyes, A; Fletcher, J; et al. "Deletion of the alpha(1,3)galactosyl transferase (GGTA1) gene and the prion protein (PrP) gene in sheep". Nature Biotechnology 19 6 (2001): 559-562.
    10.1038/89313
  79. De Sousa, PA; Harkness, L; King, T; Walker, SK; Wilmut, I; Young, LE. "Evaluation of gestational deficiencies in cloned sheep fetuses and placentae". Biology of Reproduction 65 1 (2001): 23-30.
    10.1095/biolreprod65.1.23
  80. De Sousa, PA; Natale, DR; Watson, AJ; Westhusin, ME. "Sensitivity of bovine blastocyst gene expression patterns to culture environments assessed by differential display RT-PCR". Reproduction 122 5 (2001): 687-693.
    10.1530/rep.0.1220687
  81. Wrenzycki C; De Sousa P; Overström EW; Duby RT; Herrmann D; Watson AJ; Niemann H; O'Callaghan D; Boland MP. "Effects of superovulated heifer diet type and quantity on relative mRNA abundances and pyruvate metabolism in recovered embryos.". (2000): http://europepmc.org/abstract/med/10793627.
    10.1530/jrf.0.1180069
  82. Boland, MP; De Sousa, P; Duby, RT; Herrmann, D; Niemann, H; O'Callaghan, D; Overstrom, EW; Watson, AJ; Wrenzycki, C. "Effects of superovulated heifer diet type and quantity on relative mRNA abundances and pyruvate metabolism in recovered embryos". Journal of Reproduction and Fertility 118 1 (2000): 69-78.
    10.1530/reprod/118.1.69
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