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José Pedro Sequeira holds a degree in Genetics and Biotechnology (2019) by University of Trás-os-Montes and Alto Douro and a Master Degree in Oncology (2021) granted by the School of Medicine & Biomedical Sciences of University of Porto. His Master dissertation was untitled "LiKidMiRS: unveiling the diagnostic potential of liquid biopsy-based circulating microRNAs in renal cell tumors" and was graded 20/20 by the MSc's jury. JP Sequeira is a Research Assistant in the Cancer Biology & Epigenetics Group from the Research Center of Portuguese Oncology Institute of Porto, with a fellowship from Portuguese League Against Cancer - North Delegation with the work entitled "Circulating Long Non-coding RNAs: developing a Liquid Biopsy-based Test for Prostate Cancer Clinical Management". In his curriculum has 10 articles peer-review publications, 2 of them as first author. Moreover, Sequeira is author or co-author of 10 poster presentations and 5 oral communications. JP Sequeira collaborated in 2 events organization and participated in more than 20 events. In addition, he still has 3 awards/distinctions on his curriculum. His present research is focused in circulating epigenetics biomarkers in cancer, namely miRNAs, lncRNAs and DNA methylation-based biomarkers.
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Identification

Personal identification

Full name
José Pedro Leite Sequeira

Citation names

  • José Pedro Sequeira
  • JP Sequeira
  • Sequeira, JP

Author identifiers

Ciência ID
FE1E-E1CF-6983
ORCID iD
0000-0002-4687-3347

Email addresses

  • joseplsequeira@gmail.com (Personal)

Knowledge fields

  • Medical and Health Sciences - Basic Medicine
  • Medical and Health Sciences - Clinical Medicine - Oncology
  • Medical and Health Sciences - Health Sciences - Health Policy and Services
  • Medical and Health Sciences - Basic Medicine - Human Genetics
  • Natural sciences - Biological Sciences - Molecular Biology
Education
Degree Classification
2019 - 2021
Concluded
 Oncologia (Mestrado)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
"LiKidMiRS: unveiling the diagnostic potential of liquid biopsy-based circulating microRNAs in renal cell tumors" (THESIS/DISSERTATION)
 19
2016 - 2019
Concluded
 Genética e Biotecnologia (Licenciatura)
Universidade de Trás-os-Montes e Alto Douro, Portugal
"Diffuse large B-cell lymphoma: unveiling the putative lymphomagenic signalosome crosstalk with histone modifiers" (THESIS/DISSERTATION)
 16
2013 - 2016
Concluded
 Curso Científico-Humanístico de Ciências e Tecnologias (Ensino secundário)
Agrupamento de Escolas Professor António da Natividade, Portugal
 19
Affiliation

Science

Category
Host institution 		Employer
2022/01/01 - Current Research Assistant (Research) Liga Portuguesa Contra o Cancro Núcleo Regional do Norte, Portugal
Instituto Português de Oncologia do Porto Francisco Gentil Centro de Investigação, Portugal
2019/02 - 2021/12 Research Trainee (Research) Instituto Português de Oncologia do Porto Francisco Gentil Centro de Investigação, Portugal
2018/07 - 2018/07 Research Trainee (Research) Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2016/08 - 2016/08 Research Trainee (Research) Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
Projects

Grant

Designation Funders
2022/01/01 - Current Circulating Long Non-coding RNAs: developing a Liquid Biopsy-based Test For Prostate Cancer Clinical Management
Bolsas LPCC-NRN 2022
Research Fellow
Instituto Português de Oncologia do Porto Francisco Gentil Centro de Investigação, Portugal
 Liga Portuguesa Contra o Cancro Núcleo Regional do Norte
Ongoing
Outputs

Publications

Conference poster
  1. José Pedro Sequeira; Constâncio, Vera; Salta, S; Brito-Rocha, Tiago; Carvalho-Maia, Carina; BRAGA, ISAAC; Henrique, Rui; Jerónimo, Carmen. Corresponding author: Jerónimo, Carmen. "KidLBMiRs: unveiling the diagnosis world of circulating microRNAs in renal cell tumors based liquid biopsies by ddPCR". Paper presented in II ASPIC-ASEICA International Meeting - Current Trends in Precision Medicine in Cancer, 2022.
  2. Salta, S; Maia-Moço, L; Estevão-Pereira, Helena; Sequeira, JP; Vieira, R; BARTOSCH, CARLA; Petronilho, S; et al. Corresponding author: Jerónimo, Carmen. "DNA methylation markers as potential tool for colposcopy referral in hr-HPV positive women enrolled in cervical cancer screening program". Paper presented in II ASPIC-ASEICA International Meeting - Current Trends in Precision Medicine in Cancer, 2021.
  3. José Pedro Sequeira; Lobo, João; Constâncio, Vera; Carvalho-Maia, Carina; BRAGA, ISAAC; Maurício, Joaquina; Henrique, Rui; Jerónimo, Carmen. Corresponding author: Jerónimo, Carmen. "ddPCR: liquid biopsy-based miRNA detection concept in a clinical context". Paper presented in 16th YES Meeting, CIM - Centro de Investigação Médica, FMUP - Faculdade de Medicina da Universidade do Porto, 2021.
  4. Teixeira-Marques, A; Monteiro-Reis, Sara; Constâncio, Vera; Sequeira, JP; Felizardo, Diana; Carvalho-Maia, Carina; Freitas, Rui; et al. Corresponding author: Jerónimo, Carmen. "Urinary Extracellular Vesicles: A comparison between isolation methods". Paper presented in 1st Meeting of Portuguese Network on Extracellular Vesicles, Porto, 2021.
  5. Cláudia Martins-Lima; Lobo, João; Constâncio, Vera; Catarina Guimarães-Teixeira; Vera Miranda-Gonçalves; Monteiro-Reis, Sara; Lameirinhas, Ana; et al. Corresponding author: Jerónimo, Carmen. "EMT impact in bladder cancer progression: understanding the cadherins´ role and their regulatory mechanisms". Paper presented in 27th Porto Cancer Meeting, 2021.
  6. Petronilho, S; Sequeira, JP; Paulino, S; Chacim, Sérgio; Lobo, João; Jerónimo, Carmen; Henrique, Rui. Corresponding author: Henrique, Rui. "Prognostic value of histone modifying enzyme EZH2 and histone mark H3K4me3 in R-CHOP-treated diffuse large B-cell lymphoma". Paper presented in 32nd Congress of the ESP and XXXIII International Congress of the IAP, 2020.
  7. José Pedro Sequeira; Quintas-Gonçalves, Joana; Chaves, Raquel. Corresponding author: Chaves, Raquel. "A importância da proteína p53 na apoptose e no desenvolvimento tumoral". Paper presented in XI Encontro Nacional de Estudantes de Bioquímica, 2018.
  8. Sequeira, JP; Quintas-Gonçalves, Joana; Chaves, Raquel. Corresponding author: Chaves, Raquel. "A importância da proteína p53 na apoptose e no desenvolvimento tumoral". Paper presented in X Jornadas de Genética e Biotecnologia, 2018.
  9. Quintas-Gonçalves, Joana; Sequeira, JP; Chaves, Raquel. Corresponding author: Chaves, Raquel. "Tumores de mama Her2 positivos e terapias disponíveis: uma abordagem mecanicista". Paper presented in XI Encontro Nacional de Estudantes de Bioquimica, 2018.
  10. Quintas-Gonçalves, Joana; Sequeira, JP; Chaves, Raquel. Corresponding author: Chaves, Raquel. "Tumores de mama Her2 positivos e terapias disponíveis: uma abordagem mecanicista". Paper presented in X Jornadas de Genética e Biotecnologia, 2018.
Journal article
  1. José Pedro Sequeira; Daniela Barros-Silva; Patrícia Ferreira-Torre; Sofia Salta; Isaac Braga; João Carvalho; Rui Freitas; Rui Henrique; Carmen Jerónimo. "OncoUroMiR: Circulating miRNAs for Detection and Discrimination of the Main Urological Cancers Using a ddPCR-Based Approach". International Journal of Molecular Sciences (2023): https://doi.org/10.3390/ijms241813890.
    10.3390/ijms241813890
  2. Guimarães-Teixeira, Catarina; Lobo, João; Miranda-Gonçalves, Vera; Barros-Silva, Daniela; Martins-Lima, Cláudia; Monteiro-Reis, Sara; Sequeira, José Pedro; et al. "Downregulation of m 6 A writer complex member METTL14 in bladder urothelial carcinoma suppresses tumor aggressiveness". Molecular Oncology (2022): http://dx.doi.org/10.1002/1878-0261.13181.
    10.1002/1878-0261.13181
  3. José Pedro Sequeira; Vera Constâncio; Sofia Salta; João Lobo; Daniela Barros-Silva; Carina Carvalho-Maia; Jéssica Rodrigues; et al. "LiKidMiRs: A ddPCR-Based Panel of 4 Circulating miRNAs for Detection of Renal Cell Carcinoma". Cancers (2022): https://doi.org/10.3390/cancers14040858.
    10.3390/cancers14040858
  4. Cláudia Martins-Lima; Vera Miranda-Gonçalves; João Lobo; Vera Constâncio; Pedro Leite-Silva; Catarina Guimarães-Teixeira; Sara Monteiro-Reis; et al. "Cadherin switches during epithelial-mesenchymal transition: CDH4/RCAD downregulation reduces bladder cancer progression". Cellular Oncology (2022): https://doi.org/10.1007/s13402-021-00657-2.
    10.1007/s13402-021-00657-2
  5. Sequeira, J.P.; Lobo, J.; Constâncio, V.; Brito-Rocha, T.; Carvalho-Maia, C.; Braga, I.; Maurício, J.; Henrique, R.; Jerónimo, C.. "DigiMir Test: Establishing a Novel Pipeline for MiR-371a Quantification Using Droplet Digital PCR in Liquid Biopsies From Testicular Germ Cell Tumor Patients". Frontiers in Oncology 12 (2022): http://www.scopus.com/inward/record.url?eid=2-s2.0-85133412256&partnerID=MN8TOARS.
    10.3389/fonc.2022.876732
  6. Sara Petronilho; José Pedro Sequeira; Sofia Paulino; Paula Lopes; Susana Lisboa; Sérgio Chacim; João Lobo; et al. "Prognostic Value of Histone Modifying Enzyme EZH2 in RCHOP-Treated Diffuse Large B-Cell Lymphoma and High Grade B-Cell Lymphoma". Journal of Personalized Medicine (2021): https://doi.org/10.3390/jpm11121384.
    10.3390/jpm11121384
  7. José Pedro Sequeira; Vera Constâncio; João Lobo; Rui Henrique; Carmen Jerónimo. "Unveiling the World of Circulating and Exosomal microRNAs in Renal Cell Carcinoma". Cancers 13 21 (2021): 5252-5252. https://doi.org/10.3390/cancers13215252.
    10.3390/cancers13215252
  8. Salta, Sofia; Maia-Moço, Leonardo; Estevão-Pereira, Helena; Sequeira, José Pedro; Vieira, Renata; Bartosch, Carla; Petronilho, Sara; et al. "Performance of DNA methylation-based biomarkers in the cervical cancer screening program of northern Portugal: A feasibility study". International Journal of Cancer 149 11 (2021): 1916-1925. http://dx.doi.org/10.1002/ijc.33778.
    10.1002/ijc.33778
  9. João Lobo; Vera Constâncio; Catarina Guimarães-Teixeira; Pedro Leite-Silva; Vera Miranda-Gonçalves; José Pedro Sequeira; Laura Pistoni; et al. "Promoter methylation of DNA homologous recombination genes is predictive of the responsiveness to PARP inhibitor treatment in testicular germ cell tumors". Molecular Oncology 15 4 (2021): 846-865. https://doi.org/10.1002/1878-0261.12909.
    10.1002/1878-0261.12909
  10. José Pedro Sequeira. "Extracellular Vesicles Enriched in hsa-miR-301a-3p and hsa-miR-1293 Dynamics in Clear Cell Renal Cell Carcinoma Patients: Potential Biomarkers of Metastatic Disease". Cancers (2020): http://dx.doi.org/10.3390/cancers12061450.
    10.3390/cancers12061450
  11. Francisca Dias; Ana L Teixeira; Inês Nogueira; Mariana Gomes Morais; Joana Maia; Cristian Bodo; Marta Ferreira; et al. "Plasma Extracellular Vesicle-Derived TIMP-1 mRNA as a Prognostic Biomarker in Clear Cell Renal Cell Carcinoma: A Pilot Study". International Journal of Molecular Sciences (2020): https://www.mdpi.com/1422-0067/21/13/4624.
    10.3390/ijms21134624
  12. Sequeira, José. "The inflammation-related biomarker CXCR7 independently predicts patient outcome after radical prostatectomy". Urologic Oncology: Seminars and Original Investigations (2020): http://dx.doi.org/10.1016/j.urolonc.2020.03.004.
    10.1016/j.urolonc.2020.03.004
Thesis / Dissertation
  1. "LiKidMiRS: unveiling the diagnostic potential of liquid biopsy-based circulating microRNAs in renal cell tumors". Master, Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, 2022.
  2. "Diffuse large B-cell lymphoma: unveiling the putative lymphomagenic signalosome crosstalk with histone modifiers". Degree, Universidade de Trás-os-Montes e Alto Douro, 2019.
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2021/10/20 Triage of hr-HPV positive women enrolled in cervical cancer screening program: DNA methylation markers as potential tool The Epigenome in Human Health and Diseases Conference – Autumn school
FEBS (France)
2021/09/24 DigiMir: pipeline para a quantificação de miR-371a em pacientes com tumores de células germinativas do testículo através de droplet digital PCR 3º Encontro Nacional de Jovens investigadores em Oncologia
Liga Portuguesa Contra o Cancro – Núcleo Regional do Norte (Porto, Portugal)
2021/06/05 DdPCR pipeline optimization for liquid biopsy based miRNA detection in a clinical context IJUP’21 – 14th Youth Research Meeting of the University of Porto
University of Porto (Porto, Portugal)
2021/02/22 KidLBmiRs: Kidney Tumors subtyping characterization using microRNAs based Liquid Biopsies 5th Symposium of the MSc in Oncology
ICBAS-IPO (Porto, Portugal)
2020/09/24 Uncovering the mechanisms of cadherins´ expression regulation in bladder cancer: impact in epithelialmesenchymal transition 2º Encontro Nacional de Jovens Investigadores em Oncologia
Liga Portuguesa Contra o Cancro – Núcleo Regional do Norte (Porto, Portugal)

Event organisation

Event name
Type of event (Role) 		Institution / Organization
2021 - 2021 Organizing Committee of "5th Symposium of the MSc in Oncology" (2021 - 2021)
Symposium (Member of the Organising Committee)
 Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2021 - 2021 Collaborator in Organization of "European Cancer Research Summit 2021" (2021 - 2021)
Conference (Other)
 Instituto Português de Oncologia do Porto Francisco Gentil EPE, Portugal

Event participation

Activity description
Type of event 		Event name
Institution / Organization
2021 - 2021 Attendance in "UROwebinar: Future roles for liquid biopsies in clinical decision making in uro-oncology"
Other
 UROwebinar: Future roles for liquid biopsies in clinical decision making in uro-oncology
European Association of Urology, Netherlands
2021 - 2021 Attendance in "IJUP21 - 14th Youth Research Meeting of the University of Porto"
Meeting
 IJUP21 - 14th Youth Research Meeting of the University of Porto
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2021 - 2021 Attendance in "16th YES Meeting"
Meeting
 16th YES Meeting
Universidade do Porto Faculdade de Medicina, Portugal
2021 - 2021 Attendance in "3º Encontro Nacional de Jovens investigadores em Oncologia"
Conference
 3º Encontro Nacional de Jovens investigadores em Oncologia
Liga Portuguesa Contra o Cancro – Núcleo Regional do Norte, Portugal
2021 - 2021 Attendance in "II ASPIC-ASEICA International Meeting - Current Trends in Precision Medicine in Cancer"
Meeting
 II ASPIC-ASEICA International Meeting - Current Trends in Precision Medicine in Cancer
Associação Portuguesa de Investigação em Cancro, Portugal
2021 - 2021 Attendance in "27th Porto Cancer Meeting"
Meeting
 27th Porto Cancer Meeting
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2020 - 2020 Attendance in "First Workshop of the Portuguese Network on Extracellular Vesicles: EVs in Health and Disease"
Workshop
 First Workshop of the Portuguese Network on Extracellular Vesicles: EVs in Health and Disease
Portuguese Network on Extracellular Vesicles, Portugal
2020 - 2020 Attendance at "ISLB Annual Congress 2020"
Congress
 ISLB Annual Congress 2020
International Society of Liquid Biopsy, Spain
2020 - 2020 Attendance in "Animal free hydrogel - Taking you from cell to therapy"
Workshop
 Animal free hydrogel - Taking you from cell to therapy
VWR International, United Kingdom
2020 - 2020 Attendance in "4th Symposium of the MSc in Oncology"
Symposium
 4th Symposium of the MSc in Oncology
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2019 - 2019 Attendance in "3rd Symposium of the MSc in Oncology"
Symposium
 3rd Symposium of the MSc in Oncology
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2018 - 2018 Attendance in "IX Forum on Pharmacological Research: from isolation to in vitro evaluation (8 Hours)"
Conference
 IX Forum on Pharmacological Research: from isolation to in vitro evaluation (8 Hours)
Universidade de Trás-os-Montes e Alto Douro, Portugal
2018 - 2018 Attendance in "BiotechHealth Symposium - Science & Co.: Bridging the Gap, BiotechHealth - International Doctoral Programme"
Symposium
 BiotechHealth Symposium - Science & Co.: Bridging the Gap, BiotechHealth - International Doctoral Programme
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2018 - 2018 Attendance at "Annual Meeting 2018 - Portuguese Society of Genetics"
Meeting
 Annual Meeting 2018 - Portuguese Society of Genetics
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2018 - 2018 Attendance in Workshop "Soft Skills: Scientific Writing"
Workshop
 XI National Meeting of Biochemistry Students
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2018 - 2018 Attendance in Workshop “How to make effective presentations”
Workshop
 XI National Meeting of Biochemistry Students
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2018 - 2018 Attendance at "XI National Meeting of Biochemistry Students"
Meeting
 XI National Meeting of Biochemistry Students
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2018 - 2018 Attendance in Workshop “Sanger sequencing and electropherogram reading”
Workshop
 X Conference on Genetics and Biotechnology
Universidade de Trás-os-Montes e Alto Douro, Portugal
2018 - 2018 Attendance in "X Conference on Genetics and Biotechnology"
Conference
 X Conference on Genetics and Biotechnology
Universidade de Trás-os-Montes e Alto Douro, Portugal
2017 - 2017 Attendance in "IX Conference on Genetics and Biotechnology"
Conference
 IX Conference on Genetics and Biotechnology
Universidade de Trás-os-Montes e Alto Douro, Portugal
2017 - 2017 Attendance to "Science and Technology Week"
Exhibition
 Science and Technology Week
Universidade de Trás-os-Montes e Alto Douro, Portugal
2017 - 2017 Attendance to Workshop "Communication and Networking Techniques"
Workshop
 TEDxUniversityofPorto
Universidade do Porto Faculdade de Economia, Portugal
2017 - 2017 Attendance in "TEDxUniversityofPorto"
Meeting
 TEDxUniversityofPorto
Universidade do Porto Faculdade de Economia, Portugal
2016 - 2016 Basic Life Support Training
Workshop
Associação Cultural, Recreativa e Social de Loivos da Ribeira, Portugal
2015 - 2015 National Youth Deputy for Vila Real District
Other
 Parlamento dos Jovens
Assembleia da República, Portugal
2014 - 2014 National Youth Deputy for Vila Real District
Other
 Parlamento dos Jovens
Assembleia da República, Portugal

Association member

Society Organization name Role
2020 - Current The European Association for Cancer Research (EACR) Member
2020 - Current Associação Portuguesa de Investigação em Cancro (ASPIC) Member
Distinctions

Award

2021 Oncology & Molecular Biology Poster Session Honorable Mention
Universidade do Porto Faculdade de Medicina, Portugal
2016 2nd Award Ciência Viva - All Time GABBAs
2016 Prémio Professor António da Natividade
Agrupamento de Escolas Professor António da Natividade, Portugal