???global.info.a_carregar???
Identificação

Identificação pessoal

Nome completo
Pedro Ricardo Lucas Perdigão

Nomes de citação

  • Perdigão, PRL
  • Perdigão, P
  • Pedro Perdigão

Identificadores de autor

Ciência ID
0F1E-EFD6-AF01
ORCID iD
0000-0003-2943-3515
Formação
Grau Classificação
2020/11/09 - 2020/11/20
Concluído
Modular Training in Laboratory Animal Science (Curso médio)
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2012/01/01 - 2017/06/20
Concluído
Farmácia (Doutoramento)
Universidade de Lisboa Faculdade de Farmácia, Portugal
"Gene-targeted strategies to eliminate HIV latent cells through synthetic activators and suicide lentivectors" (TESE/DISSERTAÇÃO)
Aprovado com distinção e louvor
2009/09/20 - 2011/11/21
Concluído
Biologia Molecular e Genética (Mestrado)
Universidade de Lisboa Faculdade de Ciências, Portugal
"Specific elimination of HIV-1 infected cells using a Tat/Rev-dependent circuit" (TESE/DISSERTAÇÃO)
17 (0-20)
2005/09/20 - 2009/07/09
Concluído
Bioquímica (Licenciatura)
Universidade de Lisboa Faculdade de Ciências, Portugal
15 (0-20)
Percurso profissional

Ciência

Categoria Profissional
Instituição de acolhimento
Empregador
2019/07/15 - Atual Pós-doutorado (Investigação) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2017/11/15 - 2019/07/12 Pós-doutorado (Investigação) University College London Institute of Ophthalmology, Reino Unido
University College London Institute of Ophthalmology, Reino Unido
2010/08/01 - 2017/06/20 Investigador (Investigação) Universidade de Lisboa Instituto de Investigação do Medicamento, Portugal
Universidade de Lisboa Faculdade de Farmácia, Portugal
2013/11/07 - 2014/10/17 Investigador visitante (Investigação) Scripps Research Institute, Estados Unidos
Scripps Research Institute, Estados Unidos

Outros

Categoria Profissional
Instituição de acolhimento
Empregador
2020/03 - 2020/06 Research volunteer for COVID-19 task force Universidade de Coimbra, Portugal
Projetos

Bolsa

Designação Financiadores
2024/01/01 - Atual GeneT: The Gene Therapy CoE at the Center of Portugal
Investigador
Universidade de Coimbra, Portugal
European Commission
Em curso
2024/03/01 - 2025/02/28 Engineering RNA-Targeting CRISPR/Cas13 for the Treatment of Spinocerebellar Ataxia Type 3
25124
Investigador responsável
Universidade de Coimbra, Portugal
AFM-Téléthon
2022/01/01 - 2023/12/31 Genome editing to rescue Machado-Joseph disease by alternative splicing
EXPL/MED-NEU/0936/2021
Investigador responsável
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
2020/06/01 - 2023/05/31 Rescue of inherited retinal degeneration by CRISPR base editing
5129 / 5130
Co-Applicant
University College London Institute of Ophthalmology, Reino Unido
Fight for Sight Foundation
Em curso
2022 - 2023 (Less)PlasticBrain: Bio Replacing Plasticizers - Impact in the Human Brain Development
Investigador
Universidade de Coimbra, Portugal
Banco Santander Portugal
Concluído
2022 - 2023 (Less)PlasticBrain: Bio Replacing Plasticizers - Impact in the Human Brain Development Banco Santander Portugal
2016/01/01 - 2016/06/30 HIV-1 ERADICATION BY SITE-DIRECTED INTEGRATION
Bolseiro de Investigação
Fundação para a Ciência e a Tecnologia
Concluído
2014/03 - 2016/02 An innovative approach to deliver dual-targeting HIV entry inhibitors using cationic liposomes
Investigador
Fundação para a Ciência e a Tecnologia
2014/03 - 2015/12 HIV-1 ERADICATION BY SITE-DIRECTED INTEGRATION
Investigador
Fundação para a Ciência e a Tecnologia
2012/01/01 - 2015/12 ERADICATION OF HIV-1 INFECTED CELLS BY TARGETING NANOPARTICLES CARRYING THERAPEUTIC PLASMIDS
info:eu-repo/grantAgreement/FCT/SFRH/SFRH2%FBD2%F819412%F2011/PT
Bolseiro de Doutoramento
Fundação para a Ciência e a Tecnologia
Concluído
2013/01 - 2015/03 Strategic Project - UI 775 - 2013-2014 Fundação para a Ciência e a Tecnologia
2012/01/01 - 2012/12/31 Nanotechnology against viral latency: Sensor strategies to eliminate HIV-1 infected cells.
Universidade de Lisboa Instituto de Investigação do Medicamento, Portugal
Bill & Melinda Gates Foundation
Concluído

Projeto

Designação Financiadores
2023/08/01 - Atual CiNTech - Technological Hub for Innovation, Translation and Industrialization of Complex Injectable Drugs
C644865576-00000005
Investigador
Universidade de Coimbra, Portugal
European Commission
Em curso
2021/10/01 - Atual Machado-Joseph Disease iPSC Cell Models for Medium Throughput Drug Screening
CT 0091995
Investigador Pós-doutorado
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Institut Servier SAS
Em curso
2018/07/01 - 2021/09/30 Blocking neurodegeneration by spreading of gene silencers
PTDC/BTM-SAL/29716/2017
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Fundação para a Ciência e a Tecnologia
Em curso
2017/11/15 - 2019/07/12 Developing a photoreceptor model of AIPL1 LCA from patient derived induced pluripotent stem cells
R170015A
Investigador Pós-doutorado
University College London Institute of Ophthalmology, Reino Unido
Moorfields Eye Hospital
Concluído
2014/03/01 - 2016/08/31 An innovative approach to deliver dual-targeting HIV entry inhibitors using cationic liposomes
HIVERA/0003/2013
Instituto de Medicina Molecular João Lobo Antunes, Portugal

Instituto de Medicina Molecular João Lobo Antunes, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
Produções

Publicações

Artigo em jornal
  1. Vinhas, AC; Ferreira, AC; Silva, C; Miranda, C; Henriques, D; Oliveira, D; Ramos, D; et al. ""Uma Viagem Rara"", PÚBLICO, 2020, https://www.publico.pt/2020/02/28/ciencia/fotogaleria/viagem-rara-400481.
Artigo em revista
  1. Sai, Hali; Ollington, Bethany; Rezek, Farah O.; Chai, Niuzheng; Lane, Amelia; Georgiadis, Tassos; Bainbridge, James; et al. "Effective AAV-mediated gene replacement therapy in retinal organoids modelling AIPL1-associated LCA4". Molecular Therapy - Nucleic Acids (2024): 102148. http://dx.doi.org/10.1016/j.omtn.2024.102148.
    Publicado • 10.1016/j.omtn.2024.102148
  2. Benati, D; Leung, A; Perdigão, P; Toulis, V; van der Spuy, J; Recchia, A. "Induced Pluripotent Stem Cells and Genome-Editing Tools in Determining Gene Function and Therapy for Inherited Retinal Disorders". International Journal of Molecular Sciences 23 23 (2023): 15276. https://www.mdpi.com/1422-0067/23/23/15276.
    Acesso aberto • Publicado
  3. Martins, Mariana; Oliveira, Ana Rafaela; Martins, Solange; Vieira, José Pedro; Perdigão, Pedro; Fernandes, Ana Rita; de Almeida, Luís Pereira; et al. "A Novel Genetic Variant in MBD5 Associated with Severe Epilepsy and Intellectual Disability: Potential Implications on Neural Primary Cilia". International Journal of Molecular Sciences 24 16 (2023): 12603. http://dx.doi.org/10.3390/ijms241612603.
    Publicado • 10.3390/ijms241612603
  4. Afanasyeva, Tess A.V.; Athanasiou, Dimitra; Perdigao, Pedro R.L.; Whiting, Kae R.; Duijkers, Lonneke; Astuti, Galuh D.N.; Bennett, Jean; et al. "CRISPR-Cas9 correction of a nonsense mutation in LCA5 rescues lebercilin expression and localization in human retinal organoids". Molecular Therapy - Methods & Clinical Development 29 (2023): 522-531. http://dx.doi.org/10.1016/j.omtm.2023.05.012.
    Publicado • 10.1016/j.omtm.2023.05.012
  5. Pedro Perdigão; Bethany Ollington; Hali Sai; Amy Leung; Almudena Sacristan-Reviriego; Jacqueline van der Spuy. "Retinal Organoids from an AIPL1 CRISPR/Cas9 Knockout Cell Line Successfully Recapitulate the Molecular Features of LCA4 Disease". International Journal of Molecular Sciences (2023): https://www.mdpi.com/1422-0067/24/6/5912.
    10.3390/ijms24065912
  6. Rufino-Ramos, David; Leandro, K; Perdigão, Pedro (0F1E-EFD6-AF01); O'Brien, K; Pinto, MM; Santana, MM; van Solinge, TS; et al. "Extracellular communication between brain cells through functional transfer of Cre mRNA". Molecular Therapy (2023):
    No prelo
  7. Leung, Amy; Sacristan-Reviriego, Almudena; Perdigão, Pedro R.L.; Sai, Hali; Georgiou, Michalis; Kalitzeos, Angelos; Carr, Amanda-Jayne F.; et al. "Investigation of PTC124-mediated translational readthrough in a retinal organoid model of AIPL1-associated Leber congenital amaurosis". Stem Cell Reports 17 10 (2022): 2187-2202. http://dx.doi.org/10.1016/j.stemcr.2022.08.005.
    Publicado • 10.1016/j.stemcr.2022.08.005
  8. Leung, A; Sacristan-Reviriego, A; Perdigão, Pedro (0F1E-EFD6-AF01); Sai, H; Georgiou, M; Kalitzeos, A; Carr, AJ; et al. "Translational readthrough as a potential therapeutic for AIPL1-associated Leber Congenital Amaurosis in a patient-derived iPSC-retinal organoid model". bioRxiv (2021): https://doi.org/10.1101/2021.12.17.473147.
    Publicado
  9. Sladen, Paul E.; Perdigão, Pedro R.L.; Salsbury, Grace; Novoselova, Tatiana; van der Spuy, Jacqueline; Chapple, J. Paul; Yu-Wai-Man, Patrick; Cheetham, Michael E.. "CRISPR-Cas9 correction of OPA1 c.1334G>A: p.R445H restores mitochondrial homeostasis in dominant optic atrophy patient-derived iPSCs". Molecular Therapy - Nucleic Acids 26 (2021): 432-443. http://dx.doi.org/10.1016/j.omtn.2021.08.015.
    Publicado • 10.1016/j.omtn.2021.08.015
  10. Perdigão, Pedro R.L.; Cunha-Santos, Catarina; Barbas, Carlos F.; Santa-Marta, Mariana; Goncalves, Joao. "Protein Delivery of Cell-Penetrating Zinc-Finger Activators Stimulates Latent HIV-1-Infected Cells". Molecular Therapy - Methods & Clinical Development 18 (2020): 145-158. http://dx.doi.org/10.1016/j.omtm.2020.05.016.
    Publicado • 10.1016/j.omtm.2020.05.016
  11. Cunha-Santos, C; Perdigao, PRL; Oliveira, JG; Cardoso, M; Manuel, A; Taveira, N; Goncalves, J. "Inhibition of HIV replication through siRNA carried by CXCR4-targeted chimeric nanobody.". Cellular and molecular life sciences : CMLS (2019): https://doi.org/10.1007/s00018-019-03334-8.
    Publicado • 10.1007/s00018-019-03334-8
  12. Perdigão, Pedro; Gaj, T; Santa-Marta, M; Barbas III, C; Goncalves, J. "Reactivation of Latent HIV-1 Expression by Engineered TALE Transcription Factors.". PloS one (2016): http://europepmc.org/articles/PMC4774903.
    10.1371/journal.pone.0150037
Capítulo de livro
  1. Perdigão, Pedro; van der Spuy, J. "Gene and Cell Therapy for AIPL1-Associated Leber Congenital Amaurosis: Challenges and Prospects". 2019.
    10.1007/978-3-030-27378-1_16
Poster em conferência
  1. Perdigão, P; Matos, Carlos A; Lopes, Sara; Fernandes, Ana Rita; Lopes, Miguel; Conceição, André; Faro, Rosario; et al. "Modelling Machado-Joseph Disease in patient-derived isogenic lines by CRISPR/Cas9-mediated correction of ATXN3-CAG expansion". Trabalho apresentado em ESGCT 29th Annual Congress, European Society of Gene and Cell Therapy, Edinburgh, 2022, 2022.
  2. Carvalhas de Almeida, Catarina; Pereira de Almeida, Luis ; Cavadas, Claudia; Perdigão, Pedro*; Álvaro, Ana Rita* . Autor correspondente: Perdigão, Pedro*. "Insomnia patient-derived iPSC neurons with potential for disease modeling". Trabalho apresentado em World Spleep Congress 2022, Rome, Italy, 2022.
  3. Carvalhas de Almeida, Catarina; Pereira de Almeida, Luis ; Cavadas, Claudia; Perdigão, Pedro*; Álvaro, Ana Rita* . Autor correspondente: Perdigão, Pedro*. "Establishing iPSC-derived neuronal cell model to study human insomnia pathophysiology". Trabalho apresentado em XVI Symposium of Experimental Biology and Biomedicine Doctoral Programme, University of Coimbra, Coimbra, Portugal, 2022.
  4. Perdigão, Pedro (0F1E-EFD6-AF01); Matos, Carlos A; Lopes, Sara; Fernandes, Ana Rita; Lopes, Miguel; Faro, Rosário; Santana, Magda M; Nobre, Rui Jorge; Pereira de Almeida, Luis. "Precise and scarless correction of ATXN3-CAG expansion by CRISPR/Cas9 for the generation of patient-derived MJD isogenic iPSC". Trabalho apresentado em XVII Meeting of the Portuguese Society for Neuroscience, Coimbra, Portugal, 2021.
  5. Leung, A; Sacristan-Reviriego, A; Perdigão, PRL; Bainbridge, J; Michaelides, M; van der Spuy, J. "Investigation of Ataluren (PTC124) as a potential therapeutic for LCA4 in a patient-derived iPS-retinal organoid model". Trabalho apresentado em ARVO Annual Meeting 2021, 2021.
  6. Afanasyeva, T; Perdigão, PRL; Anasthasiou, D; van der Spuy, J; Cheetham, M; Roepman, R; Collin, RWJ. "Gene-corrected iPSC Lines as a Patient-Specific Model to study LCA5-Associated Retinal Disease". Trabalho apresentado em International Society for Stem Cell Research 2020 Virtual Meeting, 2020.
  7. Perdigao, PRL; Sacristan-Reviriego, A; Jovanovic, K; Cheetham, M; Brainbridge, J; Michaelides, M; van der Spuy, J. "Using iPSC-reprogramming and CRISPR-based genome engineering to model AIPL1-associated Leber congenital amaurosis". Trabalho apresentado em Gene Editing in Ophthalmology Meeting of Macular Society 2019, Oxford, United Kingdom, 2019.
  8. Perdigão, P; Sacristan-Reviriego, A; Jovanovic, K; Cheetham, M; Brainbridge, J; Michaelides, M; van der Spuy, J. "Using iPSC-reprogramming and CRISPR-based genome engineering to model AIPL1-associated Leber congenital amaurosis". Trabalho apresentado em UCL Institute of Ophthalmology Early Career Researcher Symposium 2019, London, United Kingdom, 2019.
  9. Perdigao, PRL; Sacristan-Reviriego, A; Jovanovic, K; Cheetham, M; Brainbridge, J; Michaelides, M; van der Spuy, J. "Using iPSC-reprogramming and CRISPR-based genome engineering to model AIPL1-associated Leber congenital amaurosis.". Trabalho apresentado em XVIIIth International Symposium on Retinal Degeneration 2018, Killarney, Ireland, 2018.
  10. Perdigao, PRL; Gaj, T; Rocha, C; Barbas III, C; Santa-Marta, M; Gonçalves, J. "Gene- therapeutic approach complementing TALE activators and suicidal vectors target and eliminate HIV latent cells.". Trabalho apresentado em FASEB Science Research Conference: Genome Engineering - Cutting-Edge Research and Applications 2016, Lisbon, Portugal, 2016.
  11. Perdigão, P; Gaj, T; Rocha, C; Barbas III, C; Santa-Marta, M; Gonçalves, J. "Gene- therapeutic approach complementing TALE activators and suicidal vectors target and eliminate HIV latent cells.". Trabalho apresentado em Portuguese Annual Meeting Ciência 2016, Lisbon, Portugal, 2016.
  12. Narciso, S; Santos, AC; Perdigão, P; Gonçalves, J. "New concept of immunotherapy for manipulation of gene expression". Trabalho apresentado em 8th iMed.ULisboa Postgraduate Students Meeting 2016, Lisbon, Portugal, 2016.
  13. Perdigão, P; Gaj, T; Barbas III, C; Santa-Marta, M; Gonçalves, J. "Reactivation of latent HIV-1 expression by engineered TALE transcription factors.". Trabalho apresentado em ESGCT and FSGT Collaborative Congress 2015, Helsinki, Finland, 2015.
  14. Perdigão, Pedro; Gaj, T; Barbas III, C; Santa-Marta, M; Gonçalves, J. "Reactivation of latent HIV-1 expression by engineered TALE transcription factors.". Trabalho apresentado em 7th iMed.UL Postgraduate Students Meeting 2015, Lisbon, Portugal, 2015.
  15. Perdigão, P; Acúrcio, R; Duarte, A; São-José, C; Santa-Marta, M; Moniz-Pereira, J; Gonçalves, J. "HIV-1 latency reactivation by bacterial molecular agents derived from opportunistic infections". Trabalho apresentado em 7th iMed.UL Postgraduate Students Meeting 2015, Lisbon, Portugal, 2015.
  16. Perdigão, P; Gaj, T; Barbas III, C; Santa-Marta, M; Gonçalves, J. "Engineered artificial transcription factors as novel tools to eliminate HIV latency". Trabalho apresentado em 6th iMed.UL Postgraduate Students Meeting 2014, Lisbon, Portugal, 2014.
  17. Perdigão, P; Santa-Marta, M; Duarte, A; Gonçalves, J. "Reactivation of latent HIV-1 by extracellular factors secreted by NI-bacteria". Trabalho apresentado em 5th iMed.UL Postgraduate Students Meeting 2013, Lisbon, Portugal, 2013.
  18. Santa-Marta, M; Perdigão, P; Gonçalves, J. "Specific elimination of HIV-1 infected cells using a Tat/Rev-dependent circuit". Trabalho apresentado em A Collaborative Solution for Tropical Diseases: The Luso-American Response. Conference on HIV/AIDS research 2011, Lisbon, Portugal, 2011.
  19. Santa-Marta, M; Gardner, R; Perdigão, P; Gonçalves, J. "Identification of the signaling pathways associated with HIV Latency". Trabalho apresentado em 5th National Meeting on Cell Signalling 2011, Coimbra, Portugal, 2011.
Pré-impressão
  1. David Rufino-Ramos; Kevin Leandro; Pedro R.L. Perdigão; Killian O’Brien; Maria Manuel Pinto; Magda M. Santana; Thomas S van Solinge; et al. "Extracellular communication between brain cells through functional transfer of Cre mRNA". 2023. https://doi.org/10.1101/2023.01.29.525937.
    10.1101/2023.01.29.525937
  2. Amy Leung; Almudena Sacristan-Reviriego; Pedro R. L. Perdigão; Hali Sai; Michalis Georgiou; Angelos Kalitzeos; Amanda-Jayne F. Carr; et al. "Translational readthrough as a potential therapeutic for AIPL1-associated Leber Congenital Amaurosis in a patient-derived iPSC-retinal organoid model". 2021. https://doi.org/10.1101/2021.12.17.473147.
    10.1101/2021.12.17.473147
Resumo em conferência
  1. Perdigão, Pedro (0F1E-EFD6-AF01); Gaj, Thomas; Barbas III, Carlos F; Santa-Marta, Mariana; Gonçalves, João. "Reactivation of latent HIV-1 expression by engineered TALE transcription factors". Trabalho apresentado em European Society of Gene and Cell Therapy and Finland Society of Gene and Cell Therapy Collaborative Congress, Helsinki, 2015.
    Publicado
Tese / Dissertação
  1. Perdigão, Pedro. "Gene-targeted strategies to eliminate HIV latent cells through synthetic activators and suicide lentivectors". Doutoramento, Universidade de Lisboa Faculdade de Farmácia, 2017. http://hdl.handle.net/10451/29864.
  2. Perdigão, Pedro. "Specific elimination of HIV-1 infected cells using a Tat/Rev-dependent circuit". Mestrado, Universidade de Lisboa Faculdade de Farmácia, 2011. http://hdl.handle.net/10451/29864.

Propriedade Intelectual

Pedido provisório de patente
  1. 2023. "ATXN3-TARGETING CRISPR BASE EDITING SEQUENCES, METHODS AND USES THEREOF".
    Protegido
Atividades

Apresentação oral de trabalho

Título da apresentação Nome do evento
Anfitrião (Local do evento)
2022/11/17 Modelling Machado-Joseph Disease in Patient-Derived Isogenic Lines by CRISPR/Cas9-Mediated Correction of ATXN3-CAG Expansion 26th Annual SPGH Meeting
Portuguese Society of Human Genetics (Coimbra, Portugal)
2022/02/10 Correction of ATXN3-CAG Expansion in MJD Patient-Derived iPSC by CRISPR/Cas9 Homology-Directed Repair LII SPF Meeting
Portuguese Society of Pharmacology (Porto, Portugal)
2021/05/24 Genome Editing 2.0: CRISPR-Base Editors CNC course in Principles and Practice in Drug Development
Center for Neuroscience and Cell Biology (Coimbra, Portugal)
2021/04/21 Genome editing 2.0: CRISPR-base editors PDBEB Course - Advanced Therapies
Center for Neuroscience and Cell Biology (Coimbra, Portugal)
2020/11/24 Edição Genética: o novo motor evolutivo? Shot de Ciência
Núcleo de Estudantes de Biologia da Associação Académica de Coimbra (NEB/AAC)
2020/10/07 Genome editing methods in the context of Machado-Joseph Disease Curso de Verão – Metodologias de Investigação Científica
Instituto de Investigação Interdisciplinar da Universidade de Coimbra (iiiUC) (Coimbra, Portugal)
2017 Gene-therapeutic approach complementing TALE activators and suicidal vectors target and eliminate HIV latent cells Portuguese Annual Meeting Ciência 2017, Lisbon, Portugal
Fundação para a Ciência e a Tecnologia (Lisbon, Portugal)
2016 Gene-therapeutic approach complementing TALE activators and suicidal vectors target and eliminate HIV latent cells 8th iMed.UL Postgraduate Students Meeting 2016, Lisboa, Portugal
Research Institute for Medicines (iMed.ULisboa) (Lisbon, Portugal)
2016 Gene-therapeutic approach complementing TALE activators and suicidal vectors target and eliminate HIV latent cells FASEB Science Research Conference: Genome Engineering - Cutting-Edge Research and Applications 2016, Lisbon, Portugal
Federation of American Societies For Experimental Biology (Lisbon, Portugal)
2015 Specific elimination of latent HIV infected cells through a shock and kill suicidal gene therapy 8th Biennal Congress of the Spanish Society of Gene and Cell Therapy 2015, San Sebastian, Spain
Spanish Society of Gene and Cell Therapy (San Sebastian, Espanha)

Orientação

Título / Tema
Papel desempenhado
Curso (Tipo)
Instituição / Organização
2022/09/01 - Atual Engineering CRISPR base editors to rescue Machado-Joseph Disease in a patient-derived neuronal model.
Orientador de Miguel Dias
Biotecnologia Farmacêutica (Mestrado)
Universidade de Coimbra Faculdade de Farmácia, Portugal
2021/10/01 - Atual Assessing the impact of ATXN3 silencing in the context of a human model of Machado-Joseph Disease
Coorientador
Ciências Farmacêuticas (Doutoramento)
Universidade de Coimbra Faculdade de Farmácia, Portugal
2021/09/01 - Atual High-content screening of a novel therapy for Machado-Joseph disease in reporter gene-edited induced pluripotentstem cells
Coorientador
Ciências Farmacêuticas (Doutoramento)
Universidade de Coimbra Faculdade de Farmácia, Portugal
2021/07/05 - Atual Modulation of ATXN3 splicing by CRISPR-base editors for treatment of Machado-Joseph Disease in patient-derived cellular models
Orientador de Mário Barreira
Biotecnologia Farmacêutica (Mestrado)
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal

Universidade de Coimbra Faculdade de Farmácia, Portugal
2022/09/01 - 2022/09/30 Gene Therapy for Spinocerebellar Ataxia Type 3 (Supervisor in the scope of "Verão com Ciência 2022" training action coordinated by Fundação pela Ciência e Tecnologia)
Orientador de Miguel Dias
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2021/02/16 - 2021/08/03 Design and validation of AAV constructs for delivery of CRISPR-base editors to rescue Machado-Joseph Disease (Supervisor for the "Projecto em Biologia Aplicada" course integrated in the Biologia bachelor degree)
Orientador de Miguel Lucas
Biologia (Iniciação científica)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2020/08/01 - 2020/10/31 Genome editing methods for the generation and characterization of isogenic models of neurologic disease (Supervisor in the scope of "Verão com Ciência 2020" training action coordinated by Fundação pela Ciência e Tecnologia)
Orientador de Ana Rita Fernandes
Verão com Ciência (Iniciação científica)
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal

Participação em evento

Descrição da atividade
Tipo de evento
Nome do evento
Instituição / Organização
2022/11/14 - 2022/11/18 Quantitative Fluorescence Microscopy Course
Oficina (workshop)
VI Quantitative Fluorescence Microscopy Course
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2020/11/09 - 2020/11/20 Animal Experimentation Course IV Modular Training in Laboratory Animal Science
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2019 - 2019 Course in Creativity in research
Oficina (workshop)
University College London Human Resources, Reino Unido
2018/12/03 - 2018/12/03 Course in Quality papers
Oficina (workshop)
University College London Human Resources, Reino Unido
2018/06/14 - 2018/06/14 UCL Institute of Ophthalmology Early Career Researcher Symposium 2018
Simpósio
University College London Institute of Ophthalmology, Reino Unido
2017/01/19 - 2017/01/19 Workshop Elsevier in How to write a great research paper and get it accepted by a good journal
Oficina (workshop)
Universidade de Lisboa Instituto de Investigação do Medicamento, Portugal
2015/07/02 - 2015/07/02 Workshop Hands-On in Preventive maintenance and calibration of micropipettes
Oficina (workshop)
Universidade de Lisboa Instituto de Investigação do Medicamento, Portugal
2015/01/28 - 2015/01/28 Workshop in Design of experiments in pharmaceutical sciences research
Oficina (workshop)
Universidade de Lisboa Instituto de Investigação do Medicamento, Portugal
2014/03/13 - 2014/03/14 Workshop in BSL2 and BSL3
Oficina (workshop)
Universidade de Lisboa Instituto de Investigação do Medicamento, Portugal
2013/10/23 - 2013/10/23 Workshop in Grant writing: tips and thoughts
Oficina (workshop)
Universidade de Lisboa Instituto de Investigação do Medicamento, Portugal
2012/01/18 - 2012/01/18 Workshop in Preparation of quality figures for publication
Oficina (workshop)
Universidade de Lisboa Instituto de Investigação do Medicamento, Portugal

Júri de grau académico

Tema
Tipo de participação
Nome do candidato (Tipo de grau)
Instituição / Organização
2020/12/11 Biodistribution Analysis of Striatal and Cerebellar Administration of Modified Adeno-Associated Viral Vectors in Normal Mice
Arguente principal
Inês Torquato Afonso (Mestrado)
Universidade do Algarve, Portugal
2020/09/22 Edição de bases: o futuro da edição de genes?
Arguente principal
Irina Marques da Silva Fernandes (Mestrado)
Universidade de Coimbra Faculdade de Farmácia, Portugal
2019/09/17 Terapia Génica na Diabetes Mellitus tipo 1
Arguente principal
Marta Salvado Abreu Castelo Branco (Mestrado)
Universidade de Coimbra Faculdade de Farmácia, Portugal

Curso / Disciplina lecionado

Disciplina Curso (Tipo) Instituição / Organização
2021/03/16 - 2021/03/16 Lecture in "Genome editing 2.0: CRISPR-base editors" (Discipline- Human Molecular Genetics) Análises Clínicas (Mestrado integrado) Universidade de Coimbra Faculdade de Farmácia, Portugal
2020/04/04 - 2020/04/04 Lecture in "Genome Editing and Induced Pluripotent Stem Cells" (Discipline- Tissue Engineering and Molecular Therapy) Biotecnologia Farmacêutica (Mestrado integrado) Universidade de Coimbra Faculdade de Farmácia, Portugal
2016/05/03 - 2016/05/03 Lecture in "Genome Editing: applications for human gene therapy" (Discipline- Biotecnology Pharmaceutics) Ciências Biofarmacêuticas (Mestrado integrado) Universidade de Lisboa Faculdade de Farmácia, Portugal

Outro júri / avaliação

Descrição da atividade Instituição / Organização
2020/09/27 - 2020/09/27 Participation as a jury member for the assignment of an MSc researcher fellowship (reference: BIM-PAC CANCEL_STEM/2020/06) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal

Tutoria

Tópico Nome do aluno
2021/02 - Atual Mentor of PhD candidate Catarina Carvalhas-Almeida "Establishing iPSC-derived neuronal cell model to study human insomniapathophysiology" Catarina Carvalhas-Almeida
2019/01 - 2019/06 Mentor of PhD candidate Tess Afanasyeva in "CRISPR/Cas9-based genome editing methods for the correction of mutations associated with Leber Congential Amaurosis type 5 in patient-derived iPSCs" Tess Afanasyeva
2016/09 - 2017/06 Mentor of MSc candidate Miguel Cardoso in "Novel Strategy to Detect and Localize Latent HIV Reservoirs" Miguel Cardoso
2015/01 - 2016/12 Mentor of MSc researcher Rita Acúrcio in "Reactivation of latent HIV-1 by nocosomial bacterial infections" Rita Acúrcio
2015/09 - 2016/09 Mentor of MSc candidate Ana Sofia Narciso in "New Concept of Immunotherapy for Gene Manipulation" Ana Sofia Narciso
2015/09 - 2016/09 Mentor of MSc candidate Maria Inês Gonçalves in "O papel do DNA-PKcs na transcrição mediada pelo promotor LTR do HIV-1" Maria Inês Gonçalves
2012/09 - 2013/09 Mentor of MSc candidate Vasco Gonçalves in "Targeting Membrane Proteins of Latently Infected CD4+ T Cells Providing a Starting Point to the Eradication of HIV­1" Vasco Gonçalves
Distinções

Prémio

2022 Best Oral Communication in Basic Research, 26th Annual SPGH Meeting
Sociedade Portuguesa de Genética Humana, Portugal
2018 Travel Award - XVIIIth International Symposium on Retinal Degeneration, Killarney, Ireland
Foundation Fighting Blindness, Canadá
2016 Top Abstract Award (Oral communication) - FASEB Science Research Conference: Genome Engineering - Cutting-Edge Research and Applications, Lisbon, Portugal
Federation of American Societies for Experimental Biology, Estados Unidos
2016 Best oral communication – 8th iMed.UL Postgraduate Students Meeting, Lisboa, Portugal
Universidade de Lisboa Instituto de Investigação do Medicamento, Portugal
2015 Best oral communication – 8th Biennal Congress of the Spanish Society of Gene and Cell Therapy, San Sebastian, Spain
Spanish Society of Gene and Cell Therapy, Espanha
2011 PhD Fellowship (SFRH/BD/81941/2011)
Fundação para a Ciência e a Tecnologia, Portugal