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Diana completed a First Degree in Biochemistry, a Masters Degree in Molecular Genetics and obtained the PhD in Pharmaceutical Sciences. In 2009, Diana initiated her scientific activity on Henrique Almeida' s team, under the supervision of Alexandra Gouveia, at the Laboratory of Cellular and Molecular Biology of FMUP. There, Diana focused her study on the importance of N-terminus for the trafficking and signalling of melanocortin 5 receptor. Following 2 years, Diana was selected to work on a project entitled "Molecular and nanotecchnology-based approaches to improve the antitumor activity of small molecules", carried out at IPATIMUP, under the supervision of M. Helena Vasconcelos. Three years later, Diana initiated her PhD studies funded by FCT. During her PhD, Diana studied the importance of the cargo and the release of extracellular vesicles in tumor multidrug resistance at Cancer Drug Resistance Group, i3S/IPATIMUP. During this period, Diana was awarded with two fellowships, from GEIVEX and ME-HAD COST action, which allowed her internationalization at CIC bioGUNE in Bilbao, Spain, under the supervision of Juan M. Falcón-Pérez. In 2021, Diana was contracted as Junior Researcher at i3S/IPATIMUP investigating early molecular bases for the diagnosis and treatment of adrenocortical tumors in the era of personalized medicine. Since July 2020, Diana is an Invited Professor at Santa Maria Health School- Porto. Diana has published 24 peer-reviewed publications, most in Q1 journals, and has presented her work in national and international meetings.
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Identificação

Identificação pessoal

Nome completo
Diana Duarte de Sousa

Nomes de citação

  • Sousa, Diana

Identificadores de autor

Ciência ID
1513-B2FB-A940
ORCID iD
0000-0003-0145-2642

Domínios de atuação

  • Ciências Médicas e da Saúde - Medicina Clínica - Oncologia
  • Ciências Naturais - Ciências Biológicas - Biologia Celular
  • Ciências Naturais - Ciências Biológicas - Biologia Molecular
Formação
Grau Classificação
2019/06/07
Concluído
 Pharmaceutical Sciences (Doutoramento)
Especialização em Cellular and Molecular Biology
Universidade do Porto Faculdade de Farmácia, Portugal
"Studying extracellular vesicles from P-glycoprotein overexpressing multidrug resistant cells: new insights into their cargo and mechanisms of release" (TESE/DISSERTAÇÃO)
 Approved
2016/03/01 - 2016/03/03
Concluído
 ME-HaD Training Course : Extracellular Vesicles & Exosomes: Analysis and Properties (Outros)
Panepistemio Ioanninon, Grécia
2016/01/01 - 2016/02/28
Concluído
 Introductory animal science course-14th Edition (40 hours) (Outros)
Instituto de Biologia Molecular e Celular, Portugal
2015/04/16 - 2015/04/16
Concluído
 Flow cytometry workshop: DNA analysis and cell death course (Outros)
Instituto de Biologia Molecular e Celular, Portugal
2014/10/13 - 2014/11/03
Concluído
 Workshop: Introdução à bioestatística com recurso ao SPSS (14 hours) (Outros)
Universidade do Porto Instituto de Patologia e Imunologia Molecular, Portugal
2014/01/10 - 2014/01/10
Concluído
 Theoretical-Pratical Course: Formação em Citometria de Fluxo: BD Accuri C6 (Outros)
Universidade do Porto Faculdade de Farmácia, Portugal
2009/09 - 2011/07
Concluído
 Molecular Genetics (Mestrado)
Universidade do Minho, Portugal
 18/20
2005/09 - 2009/07
Concluído
 Biochemistry (Licenciatura)
Instituto Universitário de Ciências da Saúde, Portugal
 16/20
Percurso profissional

Ciência

Categoria Profissional
Instituição de acolhimento 		Empregador
2022 - Atual Investigador (Investigação) Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2021/05 - 2022/07 Investigador Contratado (Investigação) Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2019/07 - 2019/12 Pós-doutorado (Investigação) Universidade do Porto Instituto de Patologia e Imunologia Molecular, Portugal

Docência no Ensino Superior

Categoria Profissional
Instituição de acolhimento 		Empregador
2020/07 - Atual Professor Auxiliar Convidado (Docente Universitário) Escola Superior de Saúde de Santa Maria, Portugal
2021/02 - 2021/07 Professor Auxiliar Convidado (Docente Universitário) Universidade do Porto Faculdade de Farmácia, Portugal

Outros

Categoria Profissional
Instituição de acolhimento 		Empregador
2014/10 - 2019/06 PhD student with a grant from FCT (SFRH/BD/98054/2013) Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2016 - 2016 Visiting Researcher CIC bioGUNE, Espanha
2013/10 - 2014/09 Research grant (BI); Project: Microenvironment, metabolism and cancer (NORTE- 07-0124-FEDER) Universidade do Porto Instituto de Patologia e Imunologia Molecular, Portugal
2011/08 - 2013/09 Research grant (BI); Project: Molecular and nanotecchnology-based approaches to improve the antitumor activity of small molecules Universidade do Porto Faculdade de Farmácia, Portugal
Universidade do Porto Instituto de Patologia e Imunologia Molecular, Portugal
2009/09 - 2011/07 Master degree Student Universidade do Porto Faculdade de Medicina, Portugal
Projetos

Bolsa

Designação Financiadores
2023/02 - Atual Non-invasive liquid biopsy in the accurate diagnosis of mild and overt Cushing's Syndrome
Investigador
Em curso
2022/02/06 - 2023/02/06 Comprehensive molecular characterization of ACTH-producing cells within Primary Bilateral Macronodular Adrenal Hyperplasias originating Cushing's Syndrome
Investigador
Em curso
2013/10 - 2014/09 Microenvironment, metabolism and cancer
NORTE- 07-0124-FEDER
Bolseiro de Investigação
Concluído
2011/08 - 2013/09 Molecular and nanotecchnology-based approaches to improve the antitumor activity of small molecules
PTDC/SAU-FCT/100930/2008
Bolseiro de Investigação
Concluído

Projeto

Designação Financiadores
2023/02 - Atual COST action: Harmonizing clinical care and research on adrenal tumours in European countries
CA20122
Em curso
2021/05/24 - 2022/09/30 Early molecular bases for the diagnosis and treatment of ACTs in the era of personalized medicine
POCI- 01-0145-FEDER-031384
Investigador Pós-doutorado
Universidade do Porto Instituto de Patologia e Imunologia Molecular, Portugal
Concluído
2018/07 - 2019/06 Circulating tumor Extracellular Vesicles for minimally-invasive detection of Minimal Residual Disease in Acute Myeloid Leukemia patients
POCI-01-0145-FEDER-030457
Bolseiro de Doutoramento
Concluído
2019 - 2019 "Advancing cancer research: from basic knowledge to application"
NORTE-01-0145-FEDER-000029
Bolseiro de Pós-Doutoramento
Concluído
Produções

Publicações

Artigo em boletim informativo
  1. Diana Sousa, Hugo R. Caires, Verónica Bastos. "Early Career Investigators (ECIs) were awarded ITC Conference Grants and won Best Presentation Awards", 2021, https://stratagem-cost.eu/2021/07/early-career-investigators-ecis-were-awarded-itc-conference-grants-and-were-awarded-best-presentations/.
  2. Raquel T. Lima; Sousa, Diana; Paiva, Ana M.; Palmeira, A.; Barbosa, J.; Pedro, Madalena; Pinto, Madalena M. M.; Sousa, E. ""Modulation of autophagy by a thioxanthone decreases the viability of melanoma cells", ASPIC News , 2017", 2017, https://www.aspic.pt/pt-pt/node/5320#.ZFg_G3bMK5d.
  3. Lopes-Rodrigues, Vanessa; Di Luca, Alessio; Sousa, Diana; Seca, Hugo; Meleady, Paula; Henry, Michael; Raquel T. Lima; O'. ""Multidrug resistant tumour cells shed more microvesicle-like EVs and less exosomes than their drug-sensitive counterpart cells", ASPIC News, 2016", 2016, https://www.aspic.pt/en/noticias/multidrug-resistant-tumour-cells-shed-more-microvesicle-evs-and-less-exosomes-their-drug#.ZFg_OnbMK5c.
Artigo em revista
  1. Sousa, Diana; Pereira, Sofia S.; Pignatelli, Duarte. "Modulation of Autophagy in Adrenal Tumors". Frontiers in Endocrinology 13 (2022): http://dx.doi.org/10.3389/fendo.2022.937367.
    10.3389/fendo.2022.937367
  2. Sousa, Diana; Lima, Raquel T.; Lopes-Rodrigues, Vanessa; Gonzalez, Esperanza; Royo, Félix; Xavier, Cristina P. R.; Falcón-Pérez, Juan M.; Vasconcelos, M. Helena. "Different Ability of Multidrug-Resistant and -Sensitive Counterpart Cells to Release and Capture Extracellular Vesicles". Cells 10 11 (2021): 2886. http://dx.doi.org/10.3390/cells10112886.
    10.3390/cells10112886
  3. Klionsky, Daniel J.; Abdel-Aziz, Amal Kamal; Abdelfatah, Sara; Abdellatif, Mahmoud; Abdoli, Asghar; Abel, Steffen; Abeliovich, Hagai; et al. "Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)1". Autophagy 17 1 (2021): 1-382. http://dx.doi.org/10.1080/15548627.2020.1797280.
    10.1080/15548627.2020.1797280
  4. Sousa, Diana; Matthiesen, Rune; Lima, Raquel T.; Vasconcelos, M. Helena. "Deep Sequencing Analysis Reveals Distinctive Non-Coding RNAs When Comparing Tumor Multidrug-Resistant Cells and Extracellular Vesicles with Drug-Sensitive Counterparts". Cancers 12 1 (2020): 200. http://dx.doi.org/10.3390/cancers12010200.
    10.3390/cancers12010200
  5. Vanessa, Lopes-Rodrigues; Cristina, P. R. Xavier; Diana, Sousa; Hugo, Osório; Yehuda, G. Assaraf; Raquel, T. Lima; M., Helena Vasconcelos. "ALIX protein analysis: storage temperature may impair results". Journal of Molecular and Clinical Medicine 2 2 (2019): 29. http://dx.doi.org/10.31083/j.jmcm.2019.02.7161.
    10.31083/j.jmcm.2019.02.7161
  6. Lima, Raquel; Sousa, Diana; Gomes, Ana; Mendes, Nuno; Matthiesen, Rune; Pedro, Madalena; Marques, Franklim; et al. "The Antitumor Activity of a Lead Thioxanthone is Associated with Alterations in Cholesterol Localization". Molecules 23 12 (2018): 3301. http://dx.doi.org/10.3390/molecules23123301.
    10.3390/molecules23123301
  7. Pereira, Joana M.; Peixoto, Vanessa; Teixeira, Alexandra; Sousa, Diana; Barros, Lillian; Ferreira, Isabel C.F.R.; Vasconcelos, M. Helena. "Achillea millefolium L. hydroethanolic extract inhibits growth of human tumor cell lines by interfering with cell cycle and inducing apoptosis". Food and Chemical Toxicology 118 (2018): 635-644. http://dx.doi.org/10.1016/j.fct.2018.06.006.
    10.1016/j.fct.2018.06.006
  8. Bizarro, Ana; Sousa, Diana; Lima, Raquel; Musso, Loana; Cincinelli, Raffaella; Zuco, Vantina; De Cesare, Michelandrea; Dallavalle, Sabrina; Vasconcelos, M.. "Synthesis and Evaluation of the Tumor Cell Growth Inhibitory Potential of New Putative HSP90 Inhibitors". Molecules 23 2 (2018): 407. http://dx.doi.org/10.3390/molecules23020407.
    10.3390/molecules23020407
  9. Rodrigues, A.R.; Sousa, D.; Almeida, H.; Gouveia, A.M.. "Cell surface targeting of the Melanocortin 5 Receptor (MC5R) requires serine-rich terminal motifs". Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 1864 7 (2017): 1217-1226. http://dx.doi.org/10.1016/j.bbamcr.2017.04.004.
    10.1016/j.bbamcr.2017.04.004
  10. Lima, Raquel; Sousa, Diana; Paiva, Ana; Palmeira, Andreia; Barbosa, João; Pedro, Madalena; Pinto, Madalena; Sousa, Emília; Vasconcelos, M.. "Modulation of Autophagy by a Thioxanthone Decreases the Viability of Melanoma Cells". Molecules 21 10 (2016): 1343. http://dx.doi.org/10.3390/molecules21101343.
    10.3390/molecules21101343
  11. Reis, Filipa S.; Sousa, Diana; Barros, Lillian; Martins, Anabela; Morales, Patricia; Ferreira, Isabel C.F.R.; Vasconcelos, M. Helena. "Leccinum vulpinum Watling induces DNA damage, decreases cell proliferation and induces apoptosis on the human MCF-7 breast cancer cell line". Food and Chemical Toxicology 90 (2016): 45-54. http://dx.doi.org/10.1016/j.fct.2016.02.005.
    10.1016/j.fct.2016.02.005
  12. Lopes-Rodrigues, Vanessa; Di Luca, Alessio; Sousa, Diana; Seca, Hugo; Meleady, Paula; Henry, Michael; Lima, Raquel T.; O'Connor, Robert; Vasconcelos, M. Helena. "Multidrug resistant tumour cells shed more microvesicle-like EVs and less exosomes than their drug-sensitive counterpart cells". Biochimica et Biophysica Acta (BBA) - General Subjects 1860 3 (2016): 618-627. http://dx.doi.org/10.1016/j.bbagen.2015.12.011.
    10.1016/j.bbagen.2015.12.011
  13. Lopes-Rodrigues, Vanessa; Di Luca, Alessio; Sousa, Diana; Seca, Hugo; Meleady, Paula; Henry, Michael; Lima, Raquel T.; O’Connor, Robert; Vasconcelos, M. Helena. "Data supporting the shedding of larger extracellular vesicles by multidrug resistant tumour cells". Data in Brief 6 (2016): 1023-1027. http://dx.doi.org/10.1016/j.dib.2016.02.004.
    10.1016/j.dib.2016.02.004
  14. Barbosa, João; Lima, Raquel; Sousa, Diana; Gomes, Ana; Palmeira, Andreia; Seca, Hugo; Choosang, Kantima; et al. "Screening a Small Library of Xanthones for Antitumor Activity and Identification of a Hit Compound which Induces Apoptosis". Molecules 21 1 (2016): 81. http://dx.doi.org/10.3390/molecules21010081.
    10.3390/molecules21010081
  15. Sousa, Diana; Lima, Raquel T.; Vasconcelos, M. Helena. "Intercellular Transfer of Cancer Drug Resistance Traits by Extracellular Vesicles". Trends in Molecular Medicine 21 10 (2015): 595-608. http://dx.doi.org/10.1016/j.molmed.2015.08.002.
    10.1016/j.molmed.2015.08.002
  16. Bizarro, Ana; Ferreira, Isabel; Sokovic, Marina; van Griensven, Leo; Sousa, Diana; Vasconcelos, M.; Lima, Raquel. "Cordyceps militaris (L.) Link Fruiting Body Reduces the Growth of a Non-Small Cell Lung Cancer Cell Line by Increasing Cellular Levels of p53 and p21". Molecules 20 8 (2015): 13927-13940. http://dx.doi.org/10.3390/molecules200813927.
    10.3390/molecules200813927
  17. Lima, M. João; Sousa, Diana; Lima, Raquel T.; Carvalho, Ana Maria; Ferreira, Isabel C.F.R.; Vasconcelos, M. Helena. "Flower extracts of Filipendula ulmaria (L.) Maxim inhibit the proliferation of the NCI-H460 tumour cell line". Industrial Crops and Products 59 (2014): 149-153. http://dx.doi.org/10.1016/j.indcrop.2014.05.009.
    10.1016/j.indcrop.2014.05.009
  18. dos Santos, Tiago; Oliveira, Marta; Sousa, Diana; Lima, Raquel T.; Martins, Anabela; Ferreira, Isabel C.F.R.; Vasconcelos, M. Helena. "Suillus luteus methanolic extract inhibits proliferation and increases expression of p-H2A.X in a non-small cell lung cancer cell line". Journal of Functional Foods 6 (2014): 100-106. http://dx.doi.org/10.1016/j.jff.2013.09.023.
    10.1016/j.jff.2013.09.023
  19. Oliveira, Marta; Reis, Filipa S.; Sousa, Diana; Tavares, Catarina; Lima, Raquel T.; Ferreira, Isabel C. F. R.; Santos, Tiago dos; Vasconcelos, M. Helena. "A methanolic extract of Ganoderma lucidum fruiting body inhibits the growth of a gastric cancer cell line and affects cellular autophagy and cell cycle". Food Funct. 5 7 (2014): 1389-1394. http://dx.doi.org/10.1039/c4fo00258j.
    10.1039/c4fo00258j
  20. Azevedo, Carlos M.G.; Afonso, Carlos M.M.; Soares, José X.; Reis, Salette; Sousa, Diana; Lima, Raquel T.; Vasconcelos, M. Helena; et al. "Pyranoxanthones: Synthesis, growth inhibitory activity on human tumor cell lines and determination of their lipophilicity in two membrane models". European Journal of Medicinal Chemistry 69 (2013): 798-816. http://dx.doi.org/10.1016/j.ejmech.2013.09.012.
    10.1016/j.ejmech.2013.09.012
  21. Lopes-Rodrigues, Vanessa; Seca, Hugo; Sousa, Diana; Sousa, Emília; Lima, Raquel T.; Vasconcelos, M. Helena. "The network of P-glycoprotein and microRNAs interactions". International Journal of Cancer 135 2 (2013): 253-263. http://dx.doi.org/10.1002/ijc.28500.
    10.1002/ijc.28500
  22. Rodrigues, A R; Sousa, D; Almeida, H; Gouveia, A M. "Structural determinants regulating cell surface targeting of melanocortin receptors". Journal of Molecular Endocrinology 51 2 (2013): R23-R32. http://dx.doi.org/10.1530/jme-13-0055.
    10.1530/jme-13-0055
  23. Santos, Tiago dos; Tavares, Catarina; Sousa, Diana; Vaz, Josiana A.; Calhelha, Ricardo C.; Martins, Anabela; Almeida, Gabriela M.; Ferreira, Isabel C.F.R.; Vasconcelos, M. Helena. "Suillus luteus methanolic extract inhibits cell growth and proliferation of a colon cancer cell line". Food Research International 53 1 (2013): 476-481. http://dx.doi.org/10.1016/j.foodres.2013.05.037.
    10.1016/j.foodres.2013.05.037
  24. Azevedo, Carlos M.G.; Afonso, Carlos M.M.; Sousa, Diana; Lima, Raquel T.; Helena Vasconcelos, M.; Pedro, Madalena; Barbosa, João; et al. "Multidimensional optimization of promising antitumor xanthone derivatives". Bioorganic & Medicinal Chemistry 21 11 (2013): 2941-2959. http://dx.doi.org/10.1016/j.bmc.2013.03.079.
    10.1016/j.bmc.2013.03.079
Poster em conferência
  1. Rui Bergantim, Sara Peixoto da Silva, Joana Pereira, Vanessa Pinto, Diana Sousa, Rune Matthiesen , José E. Guimarães M.. "Identifying microRNAs as potential biomarkers of drug resistance in Multiple Myeloma patients: proof-of-concept study. 19th International Myeloma Society Annual Meeting in Los Angeles, California". 2022.
  2. D. Sousa, R. T. Lima, C. P. R. Xavier, V. Lopes-Rodrigues, E. Gonzalez, F. Royo, J. M. Falcón-Pérez, M. H. Vasconcelos#.. "Studying the ability of tumor multidrug-resistant cells and drug-sensitive counterparts to release and capture extracellular vesicles. 5th Congress of the Serbian Association for Cancer Research (SDIR)". 2021.
  3. D. Sousa, R. Rebelo #, R. T. Lima, C. P. R. Xavier, M. H. Vasconcelos.. "Drug sequestration by Extracellular Vesicles shed by multidrug resistant cells: is there a role for P-glycoprotein in this process? First Meeting of the Portuguese Network on Extracellular Vesicles (PNEV). i3S, Porto, Portugal". 2021.
  4. D. Sousa, R. T. Lima, C. P. R. Xavier#, V. Lopes-Rodrigues, E. Gonzalez , F. Royo, J. M. Falcón-Pérez, M. H. Vasconcelos. "Endocytic pathway alterations between multidrug resistant and drug sensitive cells imply different abilities of those cells to release and capture EVs. First Meeting of the Portuguese Network on Extracellular Vesicles (PNEV)". 2021.
  5. "R. Rebelo#, D. Sousa, M. H. Vasconcelos. (2020) “Optimization of an immuno-TEM protocol for analyzing P-glycoprotein on the surface of extracellular vesicles shed by drug sensitive and multidrug resistant counterpart cells” IJUP2020. Porto, Portugal". 2020.
  6. "M.J. Almeida#, D. Sousa, C. Xavier, M.H. Vasconcelos “Screening of novel compounds in pairs of drug-sensitive and multidrug resistant counterpart human tumor cell lines: identifying multidrug resistance inhibitors” IJUP2020. Porto, Portugal". 2020.
  7. "D. Sousa#, R. Matthiesen, R. T. Lima, M. H. Vasconcelos. (2019) “Deep sequencing analysis shows distinct transcripts in tumor multidrug resistant cells and their extracellular vesicles when compared with their drug-sensitive counterparts” i3S Retreat. Póvoa do Varzim, Portugal". 2019.
  8. "D. Sousa#, R. T. Lima, V. Lopes-Rodrigues, E. Gonzalez, F. Royo, J. M. Falcón-Pérez, M.H. Vasconcelos (2019) “Distinct regulation of the endocytic pathway between drug-sensitive and multidrug resistant cancer cells may influence their ability to release and capture EVs” Day of the Microbiology Lab from FFUP- Microbiology, Immunology and Oncobiology: Interdisciplinary approaches to control infectio". 2019.
  9. "D. Sousa#, R. Matthiesen, R. T. Lima, M. H. Vasconcelos (2019) “Deep sequencing analysis shows distinct transcripts in tumor multidrug resistant cells and their extracellular vesicles when compared with their drug-sensitive counterparts” Day of the Microbiology Lab from FFUP- Microbiology, Immunology and Oncobiology: Interdisciplinary approaches to control infectious diseases and cancer. Porto, Po". 2019.
  10. "D. Sousa#, R.T. Lima, V. Lopes-Rodrigues, F. Royo, J.M. Falcón-Pérez, M.H. Vasconcelos. (2018) “Understanding mechanisms of release and internalization of Extracellular Vesicles by multidrug resistant and drug-sensitive cells.”, i3S Scientific Retreat 2018. Póvoa de Varzim, Portugal.". 2018.
  11. "D. Sousa#, R.T. Lima, V. Lopes-Rodrigues, F. Royo, J.M. Fálcon-Perez, M.H. Vasconcelos. (2018) “Influence of Extracellular Vesicles to the acquisition of MDR phenotype by tumor cells.”, 1st Meeting in Vesicular Biology. Coimbra, Portugal.". 2018.
  12. "J. Pereira#, V. Peixoto, A. Teixeira, D. Sousa, I.C.F.R. Ferreira, M.H. Vasconcelos. (2018) “Achillea millefolium L. hydroethanolic extract has phenolic compounds and inhibits the growth of human tumor cell lines.”, 19th Biennial Meeting for the Society for Free Radical Research International. Lisbon, Portugal.". 2018.
  13. "D. Sousa#, R.T. Lima, V. Lopes-Rodrigues, F. Royo, J.M. Fálcon-Perez, M.H. Vasconcelos. (2017) “Is the dissemination of cancer multidrug resistance phenotype influenced by the shedding of Extracellular Vesicles by resistant tumor cells?”, 19th Euro Congress on Cancer Science and Therapy. Lisbon, Portugal.". 2017.
  14. "J. Pereira#, A. Teixeira, V. Peixoto, D. Sousa, I.C.F.R. Ferreira and M.H. Vasconcelos. (2017) “Investigating the cell growth inhibitory effect of an aqueous extract from Achillea millefolium L. in human tumor cell lines”. 19th Euro Congress on Cancer Science and Therapy, Lisbon, Portugal.". 2017.
  15. "Teixeira#, D. Sousa, C.P.R. Xavier, M.H. Vasconcelos. (2017) “Characterization of Extracellular Vesicles from a pair of drug-sensitive and drug-resistant BCR-ABL positive cell lines”, V AEICBAS Biomedical Congress, ICBAS. Porto, Portugal.". 2017.
  16. "A. Teixeira#, D. Sousa, C.P.R. Xavier, M.H. Vasconcelos. (2017) “Is there intercellular transfer of drug resistance mediated by Extracellular Vesicles in a Chronic Myeloid Leukemia cell line with mutated BCR-ABL?”, IJUP - 10th Meeting of Young Researchers of the University of Porto. Porto, Portugal.". 2017.
  17. "J. Pereira, V. Peixoto#, A. Teixeira, D. Sousa, I.C.F.R. Ferreira, M.H. Vasconcelos. (2017) “Investigating the cell growth inhibitory effect of aqueous extract from Achillea millefolium in human tumor cell lines”, IJUP - 10th Meeting of Young Researchers of the University of Porto. Porto, Portugal.". 2017.
  18. "D. Sousa#, R.T. Lima, V. Lopes-Rodrigues, F. Royo, J.M. Fálcon-Perez, M.H. Vasconcelos. (2016) “Extracellular Vesicles in the dissemination of cancer multidrug resistance.”, i3S Scientific Retreat 2016. Póvoa de Varzim, Portugal.". 2016.
  19. "F. S. Reis#, D. Sousa, A. Martins, P. Morales, I. C.F.R. Ferreira, M. H. Vasconcelos. (2016) “Leccinum vulpinum antitumor potential: which cell biological functions may be affected?” XIII Encontro de Química dos Alimentos. Porto, Portugal.". 2016.
  20. "F. S. Reis#, D. Sousa, L. Barros, A. Martins, P. Morales, I. C.F.R. Ferreira, M. H. Vasconcelos. (2016) “Leccinum vulpinum Watling: nutritional, antioxidant and antitumor potential.” 5th i3S Annual Meeting. Póvoa de Varzim, Portugal.". 2016.
  21. F. S. Reis#, D. Sousa, L. Barros, A. Martins, P. Morales, I.C.F.R. Ferreira, M. H. Vasconcelos.. "Potencial antitumoral de cogumelos silvestres do Nordeste Transmontano. e-Poster on Encontro Ciência 2016. Lisboa, Portugal.". 2016.
  22. "D. Sousa, V. Lopes-Rodrigues#, R.T. Lima, M.H. Vasconcelos. (2015) “Transfer of Drug Resistance by extracellular vesicles shed by tumor cells.”, First Iberian Meeting on Extracellular Vesicles. Porto, Portugal.". 2015.
  23. "Bizarro#, I.C.F.R. Ferreira, M. Sokovic, L. J.L.D. van Griensven, D. Sousa, M.H. Vasconcelos, R.T. Lima. (2014) “Cordyceps militaris (L.) Link fruiting body reduces NCI-H460 cellular viability through a mechanism involving p53 and p21.”, XX Encontro Luso- Galego de Química. Porto, Portugal.". 2014.
  24. "F. S. Reis#, D. Sousa, L. Barros, A. Martins, P. Morales, M. Helena Vasconcelos, I. C.F.R. Ferreira. (2014) “Nutritional, antioxidant and antitumour properties of Leccinum vulpinum Watling”. XX Encontro Luso-Galego de Química. Porto, Portugal.". 2014.
  25. "M.J. Lima#, D. Sousa, R.T. Lima, A.M. Carvalho, I.C.F.R. Ferreira, M.H. Vasconcelos. (2014) “Flower extracts of Filipendula ulmaria (L.) Maxim inhibit the cell growth of human tumour cell lines”, XX Encontro Luso- Galego de Química. Porto, Portugal.". 2014.
  26. "R.T. Lima, D. Sousa#, N. Mendes, R. Matthiesen, A.S. Gomes, M.M. Pinto, E. Sousa, M.H. Vasconcelos. (2014) “TXA1.HCl induces autophagy in NCI-H460 cells and reduces the growth of these lung cancer cells xenografted in nude mice”, i3S Scientific Retreat 2014. Póvoa de Varzim, Portugal.". 2014.
  27. "V. Lopes-Rodrigues#, A.D. Luca, D. Sousa, H. Seca, P. Meleady, M. Henry, R.T. Lima, R. O’Connor, M.H. Vasconcelos (2014) “Extracellular vesicles shed by multidrug resistant cells are bigger than their sensitive counterparts and have a cargo with a specific signature of proteins involved in their biogenesis”, i3S Scientific Retreat 2014. Póvoa de Varzim, Portugal.". 2014.
  28. "M. J. Lima, D. Sousa#, R.T. Lima, A.M. Carvalho, l.C.F.R. Ferreira, M.H. Vasconcelos (2014) “Flower extracts of Filipendula ulmaria (L.) Maxim inhibit the proliferation of human tumor cell lines”, IJUP’14- 7º Encontro de Jovens Investigadores da Universidade do Porto. Porto, Portugal.". 2014.
  29. "R. T. Lima, D. Sousa#, K. Choosang, P. Pakkong, A. Palmeira, A.M. Paiva, H. Seca, F. Cerqueira, M. Pedro, M.M. Pinto, E. Sousa, M.H. Vasconcelos. (2013) “A small molecule which affects the viability of a melanoma cell line through the induction of autophagy.” XII Porto Cancer Meeting. Porto, Portugal.". 2013.
  30. "A.S. Gomes#, T. Oliveira, D. Sousa, R.T. Lima, J.A.R. Salvador, M.H. Vasconcelos, M.M. Pinto, E. Sousa. (2013) “Studies of the possible oxidative metabolism of a promising antitumor compound.” XII Porto Cancer Meeting. Porto, Portugal.". 2013.
  31. "J.F. Barbosa#, D. Sousa, R.T. Lima, H. Bousbaa, A.S. Gomes, M.M. Pinto, E. Sousa, M.H. Vasconcelos, M. Pedro. (2013) “A potent small molecule that irreversible induces cell death in MCF-7 breast cancer cells.” XII Porto Cancer Meeting. Porto, Portugal.". 2013.
  32. "R.T. Lima, D. Sousa#, K. Choosang, P. Pakkong, A. Palmeira, A.M. Paiva, H. Seca, F. Cerqueira, M. Pedro, M.M. Pinto, E. Sousa, M.H. Vasconcelos. (2013) “A potent thioxanthone which induces autophagy and cell death in a melanoma cell line.” International Congress on Targeted AntiCancer Therapies (TAT). Paris, France.". 2013.
  33. "R.T. Lima#, D. Sousa, K. Choosang, P. Pakkong, A. Paiva, A. Palmeira, H. Seca, F. Cerqueira, M. Pedro, M.M. Pinto, E. Sousa and M.H. Vasconcelos. (2012) “Identification of a potent xanthone which induces cell death in a melanoma cell line model.” 3º Encontro Nacional de Química Terapêutica. Aveiro, Portugal.". 2012.
  34. "C.M.G. Azevedo, C.M.M. Afonso#, H. Vasconcelos, D. Sousa, R.T. Lima, M.M.M. Pinto. (2012) “Synthesis of fused 2,2-dimethyl-3,4-dihydrobenzopyran xanthones and evaluation of their growth inhibitory effect on four human tumour cell lines.” 3º Encontro Nacional de Química Terapêutica. Aveiro, Portugal.". 2012.
  35. "D. Sousa#, A. Rodrigues, H. Almeida, A. Gouveia. (2011) “Cell surface targeting of Melanocortin 5 Receptor requires a small N-terminal motif (Ser4-Leu7).” I3S Retreat. Póvoa do Varzim, Portugal.". 2011.
  36. "D. Sousa#, A. Rodrigues, H. Almeida, A. Gouveia. (2010) “Importance of amino terminus for Melanocortin 5 receptor intracellular trafficking and signalling.” Congresso Nacional da Sociedade Portuguesa de Bioquímica. Porto, Portugal.". 2010.
  37. "D. Sousa#, A. Rodrigues, H. Almeida, A. Gouveia. (2010) “Melanocortin 5 Receptor targeting to cell surface: The role of N-terminus. Young European Scientist Meeting V Edition.” Porto, Portugal.". 2010.
  38. "D. Sousa#, A. Rodrigues, H. Almeida, A. Gouveia. (2009) “Função do N-terminal do receptor 5 das melanocortinas no tráfego celular.” III Jornadas de Ciências da Saúde – Norte “Ciência: a causa das coisas”. Porto, Portugal.". 2009.
Tese / Dissertação
  1. Diana Duarte de Sousa. "Studying extracellular vesicles from P-glycoprotein overexpressing multidrug resistant cells: new insights into their cargo and mechanisms of release". Doutoramento, 2019. https://hdl.handle.net/10216/121237.
  2. "Trafficking and signalling of melanocortin 5 receptor: importance of N-terminus". Mestrado, 2011.
Website
  1. Sousa, Diana; Pereira, Sofia S.; Anabela Nunes; Pignatelli, Duarte. ADRENO +. 2021. https://adrenomais.i3s.up.pt/.
Atividades

Apresentação oral de trabalho

Título da apresentação Nome do evento
Anfitrião (Local do evento)
2023/02/05 Prevelance of bilateral adrenocortical incidentalomas: A systematic review and meta-analysis Portuguese Congress of Endocrinology 2023
2021/06/07 Next generation sequencing analysis reveals distinct transcripts and microRNAs in tumor multidrug resistant cells and extracellular vesicles when compared with their drug-sensitive counterparts" D. Sousa, R. T. Lima, R. Mathiessen, M. H. Vasconcelos (flash presentation) III International AICC Exosome meeting
2020/11/04 "Multidrug Resistant cells show different ability to release and capture EVs than their sensitive counterparts: effect of endocytic pathway regulation" D. Sousa, R. T. Lima, V. Lopes-Rodrigues, E. Gonzalez, F. Royo, J. M. Falcón-Pérez, M. H. Vasconcelos. (speed talk) CA17104 -STRATAGEM- NEW DIAGNOSTIC AND THERAPEUTIC TOOLS AGAINST MULTIDRUG RESISTANT COST WG3 meeting - Online event
2019/12/03 "Extracellular vesicles shed by tumor multidrug resistant cells: from mechanisms of release to cargo" Conferences in Oncobiology 2019/20 in Porto, Portugal
2019/11/06 "Distinct regulation of the endocytic pathway between drug-sensitive and multidrug resistant cancer cells may influence their ability to release and capture EVs." D. Sousa, R. T. Lima, V. Lopes-Rodrigues, E. Gonzalez, F. Royo, J. M. Falcón-Pérez, M. H. Vasconcelos 5th GEIVEX Symposium in Granada, Spain
2017/03/23 "Understanding the role of Extracellular Vesicles in the transfer of Multidrug Resistant traits." D. Sousa, R. T. Lima, V. Lopes-Rodrigues, E. Gonzalez, F. Royo, J. M. Falcón-Pérez, M. H. Vasconcelos 1st Euskadi Workshop on Exosomes in Bilbao, Spain
2016/03/02 "Transfer of cancer drug resistance traits by Extracellular Vesicles" D. Sousa, V. Lopes-Rodrigues, R.T. Lima, M.H. Vasconcelos COST ME-HaD Training Course on Extracellular vesicles in Ioannina, Greece

Orientação

Título / Tema
Papel desempenhado 		Curso (Tipo)
Instituição / Organização
2022/07 - Atual Tumor molecular profiling and non-invasive liquid biopsy to fine-tune adrenocortical carcinoma diagnosis
Coorientador
 PhD in Biomedical Sciences (Doutoramento)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal

Organização de evento

Nome do evento
Tipo de evento (Tipo de participação) 		Instituição / Organização
2011 - 2015 Outreach activity: “Ciência Viva” Visit to the laboratories by high school students and Future perspectives on Science (2 weeks each year) (2011 - 2015)
2013/02/21 - 2013/02/22 COST Action CM1106 Chemical Approaches to Targeting Drug Resistance in Cancer Stem Cells Secretariat (2013/02/21 - 2013/02/22)
2010 - 2010 Outreach activity: “Universidade Junior- O mundo colorido das células” Visit to the laboratories by high school students and Future perspectives on Science (during 2 weeks) (2010 - 2010)
2008/04/18 - 2008/04/19 II Jornadas de Ciências do Instituto Superior de Ciências da Saúde-Norte CSI: fronteira ficção/realidade Organizing Commitee (2008/04/18 - 2008/04/19)

Júri de grau académico

Tema
Tipo de participação 		Nome do candidato (Tipo de grau)
Instituição / Organização
2022/11/11 Determinação da atividade antitumoral de derivados quirais de xantonas e flavonoides
Arguente principal
 Flávia Patrícia Ribeiro Barbosa (Mestrado)
Universidade de Aveiro, Portugal
2022/07/21 Prognostic molecular markers in adrenocortical carcinomas
Arguente principal
 Mariana Queirós Machado (Outro)
2021/11/23 "Angiogenesis in adrenocortical tumors"
Arguente principal
 Sofia Borges Oliveira (Mestrado)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2021/11/19 "Comparação de duas metodologias para o isolamento de vesículas extracelulares de células de cancro gástrico para a deteção de STn."
Arguente principal
 Tânia Isabel Meleiro Lopes (Mestrado)
Universidade de Aveiro, Portugal
2019/12/19 "Estudo da expressão da proteína LRP1B em tumores de ovário."
Arguente principal
 Ana Isabel Cunha (Mestrado)
Universidade de Aveiro, Portugal

Arbitragem científica em conferência

Nome da conferência Local da conferência
2022/05/04 - 2022/05/06 IJUP 22 ¿ 15º Encontro de Investigação Jovem da UP_Poster evaluator

Curso / Disciplina lecionado

Disciplina Curso (Tipo) Instituição / Organização
2021 - Atual Pharmacology Nursing degree Escola Superior de Saúde de Santa Maria, Portugal
2021 - Atual Basic microbiology and antimicrobials Post-graduation in Quality and Control of Infections in Health Units Escola Superior de Saúde de Santa Maria, Portugal
2020 - Atual Microbiology (chair) Nursing degree Escola Superior de Saúde de Santa Maria, Portugal
2021 - 2021 Virology (pratical units) Msc Pharmaceutical Sciences Universidade do Porto Faculdade de Farmácia, Portugal

Expedição científica

Descrição da atividade Instituição / Organização
2023/02 - 2023/07 Guest Editor Special Issue "Molecular Research of Endocytic and Autophagic Pathways in Diseases" International Journal of Molecular Sciences (ISSN 1422-0067)

Tutoria

Tópico Nome do aluno
2019 - 2019 Master student at i3S Maria João Almeida
2019 - 2019 Trainee at i3S Rita Rebelo (MSc)
2017 - 2017 Trainee at i3S Joana Pereira (MSc)
2013 - 2014 Trainee at IPATIMUP Filipa S. Reis (MSc)
2013 - 2013 Master student at Faculty of Pharmacy of University of Porto Maria João Lima
2013 - 2013 Master student at Faculty of Pharmacy of University of Porto Marta Oliveira
Distinções

Prémio

2021 Best Flash presentation award at the AICC international meeting
2020 3rd prize for the best poster presentation (with speed talk) at CA17104 -Stratagem- New diagnostic and therapeutic tools against multidrug resistant COST WG3 meeting
2020 Co-author_Distinction for poster presentation at 13th Meeting of Young Researchers of the University of Porto
2018 Co-author_Honorable Mention for best poster at 11th Meeting of Young Researchers of the University of Porto
2017 Co-author_2nd best oral communication at the Plenary Session of 12th Young European Scientist Meeting
2017 Co-author_3rd best oral poster presentation at V AEICBAS Biomedical Congress,
2010 Best Poster at Young European Scientist Meeting- V Edition
2009 Best student of Biochemistry degree at Advanced Institute of Health Sciences – North
2009 3rd best poster at “III Jornadas de Ciências da Saúde -Norte -Ciência: a causa das coisas”

Outra distinção

2021 ITC Conference Grant from COST Action: CA17104 "New diagnostic and therapeutic tools against multidrug resistant tumors"
2017 Short-term scientific mission grant from ME-HAD (Microvesicles and Exosomes in Health and Disease) COST Action BM1202 to work at CIC bioGUNE (Bilbao, Spain
2016 Mobility fellowship from GEIVEX (Grupo Español de Innovación e Investigación en Vesículas Extracelulares) to work at CIC bioGUNE (Bilbao, Spain)
2016 Course and Travel Grant from ME-HaD for the Training Course "Extracellular Vesicles & Exosomes: Analysis and Properties”, University of Ioannina, Greece
2014 Career Grant: Individual PhD Fellowship