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His scientific interests are centered on the following topics: i) - the study of trimeric autotransporter adhesins (TAAs) as key virulence determinants in members of the Burkholderia cepacia complex (Bcc), a group of bacteria that cause infections in patients with Cystic Fibrosis. To study the contribution of these genes/proteins we take a multidisciplinary approach including molecular and cell biology, biochemistry and biophysics. We are particularly interested in defining the functions of TAAs during Bcc/host cell interactions. ii) - the study of bacterial proteins/peptides as drug candidates for cancer therapy. Studies aiming to elucidate the cellular and molecular effects of treating cancer cells with the protein azurin from P. aeruginosa, and its fragment, a cell penetrating peptide of 28 amino acids (p28). The p28 peptide completed two phase I clinical trials in US. We have paid a particular attention to the development of nano-carriers; in particular our focus relies on the design and fabrication of functionalized p28-nanoformulations capable of targeting cancer cells. iii) - the development of a non-mammalian animal model (the larvae of the greater wax moth Galleria mellonella) to dissect virulence caused by various microbes. We acquired experimental skills and made available an experimental facility at iBB including an insectarium for breeding and maintenance of larvae for in vivo studies of microbial infections. This animal model allowed us to assess the role of a number of virulence determinants of gram negative and positive bacteria.
Identificação

Identificação pessoal

Nome completo
Arsénio Carmo Sales Mendes Fialho

Identificadores de autor

Ciência ID
D912-1172-64A4

Endereços de correio eletrónico

  • afialho@tecnico.ulisboa.pt (Profissional)

Telefones

Telefone
  • 218417684 (Profissional)

Moradas

  • Instituto Superior Técnico, Departamento de Bioengenharia, Av. Rovisco Pais, 1049-001, Lisboa, Lisboa, Portugal (Profissional)

Websites

Idiomas

Idioma Conversação Leitura Escrita Compreensão Peer-review
Inglês Utilizador proficiente (C1) Utilizador proficiente (C1) Utilizador proficiente (C1) Utilizador proficiente (C1) Utilizador proficiente (C1)
Francês Utilizador independente (B2) Utilizador proficiente (C1) Utilizador independente (B1) Utilizador proficiente (C1) Utilizador independente (B2)
Formação
Grau Classificação
2011
Concluído
Biotecnologia (Título de Agregado)
Universidade de Lisboa Instituto Superior Técnico, Portugal
1990 - 1996
Concluído
Biotecnologia (Doutoramento)
Universidade de Lisboa Instituto Superior Técnico, Portugal
1982 - 1986
Concluído
Biologia (Licenciatura)
Universidade de Lisboa Faculdade de Ciências, Portugal
Percurso profissional

Docência no Ensino Superior

Categoria Profissional
Instituição de acolhimento
Empregador
2021/05/02 - Atual Professor Catedrático (Docente Universitário) Universidade de Lisboa Instituto Superior Técnico, Portugal
2004 - 2021/05/01 Professor Associado (Docente Universitário) Universidade de Lisboa Instituto Superior Técnico, Portugal
1996 - 2004 Professor Auxiliar (Docente Universitário) Universidade de Lisboa Instituto Superior Técnico, Portugal
1990 - 1996 Assistente (Docente Universitário) Universidade de Lisboa Instituto Superior Técnico, Portugal
1988 - 1990 Assistente Estagiário (Docente Universitário) Universidade de Lisboa Instituto Superior Técnico, Portugal
Projetos

Bolsa

Designação Financiadores
1987 - 1990 Produção e caracterização de exopolissacáridos de origem microbiana
JNICT BIO/61/87
JNICT BIO/61/87
Bolseiro de Iniciação Científica
N/A
Concluído

Projeto

Designação Financiadores
2021/03/01 - 2024/02/29 Ativação de bactérias dormentes com proteínas derivadas de fagos para aumentar a eficácia de antibióticos
PTDC/BIA-MIC/2312/2020
Universidade do Minho, Portugal

Universidade do Minho Centro de Engenharia Biológica, Portugal

Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
Fundação para a Ciência e a Tecnologia
Em curso
2018/12/01 - 2021/11/30 O péptido penetrador celular p28 como mediador da nano-entrega de fármacos para o tratamento do cancro
PTDC/BTM-SAL/30034/2017
Investigador responsável
Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal

Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal

Instituto Nacional de Engenharia Biomédica, Portugal
Fundação para a Ciência e a Tecnologia
Em curso
2018/10/01 - 2021/09/30 Explorando a interferência probiótica entre lactobacilos e Candida para potenciar o desenvolvimento de novas terapias antifúngicas
PTDC/BIA-MIC/31515/2017
Investigador
Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal

Universidade do Minho, Portugal

Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
Fundação para a Ciência e a Tecnologia
Em curso
2018/08/10 - 2021/08/09 Engenharia de Exossomas Inteligentes para Remoção de Proteína beta-amilóide na doença de Alzheimer.
PTDC/BTM-SAL/31057/2017
Investigador
Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal

Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
Fundação para a Ciência e a Tecnologia
Em curso
2012/02/01 - 2015/07/31 Adesinas triméricas autotransportadas associadas à virulência de Burkholderia cenocepacia: caracterização funcional, biofísica e estrutural
PTDC/BIA-MIC/118386/2010
Investigador responsável
Instituto de Biotecnologia e Bioengenharia, Portugal

Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal

Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal

Université catholique de Louvain Institut de la Matière Condensée et des Nanosciences, Bélgica
Fundação para a Ciência e a Tecnologia
Concluído
2010/01/01 - 2013/06/30 A proteína bacteriana azurina: nova abordagem terapêutica para cancro da mama com mau prognóstico
PTDC/EBB-BIO/100326/2008
Investigador responsável
Universidade de Lisboa Instituto Superior Técnico, Portugal

Instituto de Biotecnologia e Bioengenharia, Portugal

Universidade do Porto Instituto de Patologia e Imunologia Molecular, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
2008/01/01 - 2011/03/31 Elucidação do papel fundamental dos enzimas da família da RNase II no Metabolismo do RNA: Estudos Funcionais e Estruturais
PTDC/BIA-MIC/71453/2006
Investigador responsável
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Universidade de Lisboa Instituto Superior Técnico, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
2008/01/01 - 2011/03/31 Estudos Bioquímicos e Estruturais em UDP-Glucose Desidrogenases
PTDC/QUI/67925/2006
Investigador responsável
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Universidade de Lisboa Instituto Superior Técnico, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
2007/10/27 - 2010/10/26 Infecção invasiva versus infecção crónica em doentes com fibrose quística por bactérias do complexo Burkholderia cepacia: mecanismos envolvidos, em particular o papel da biossíntese de exopolissacárido
PTDC/SAU-MII/69591/2006
Investigador
Universidade de Lisboa Instituto Superior Técnico, Portugal

Universidade de Lisboa Instituto Superior Técnico, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
2005/04/01 - 2008/12/31 Biossíntese da goma bacteriana de gelano: biologia molecular, bioquímica e engenharia genética da via biossintética
POCI/BIO/58401/2004
Investigador responsável
Universidade de Lisboa Instituto Superior Técnico, Portugal

Universidade de Lisboa Instituto Superior Técnico, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
2005/09/01 - 2007/06/30 Estratégias para acelerar a biodegradação da atrazina em solos, águas e sedimentos e avaliação da ecotoxicidade deste herbicida e dos seus metabolitos (ATRAZINEBIOREM)
POCI/AMB/56039/2004
Investigador responsável
Universidade de Lisboa Instituto Superior Técnico, Portugal

Universidade de Lisboa Instituto Superior Técnico, Portugal

Universidade de Coimbra Instituto Ambiente e Vida, Portugal

Agência Portuguesa do Ambiente, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
2003 - 2006 Análise funcional de genes envolvidos na biossíntese de polissacáridos em Sinorhizobium meliloti: importância na fixação biológica do azoto
POCTI/AGG/39533/2001
POCTI/AGG/39533/2001
Investigador
N/A
Concluído
2003 - 2006 Bacterial exopolysaccharide biosynthesis: the steps following the repeat unit formation.
POCTI/BME/44441/2002
POCTI/BME/44441/2002
Investigador
N/A
Concluído
2004/10/01 - 2005/01/31 DETERMINANTES DE VIRULÊNCIA DO MICRORGANISMO BURKHOLDERIA CEPACIA E O SEU POSSÍVEL ENVOLVIMENTO NO TRATAMENTO DO CANCRO.
SFRH/BSAB/457/2004
Fundação para a Ciência e a Tecnologia
Concluído
2003 - 2004 Engenharia de polissacáridos bacterianos
POCTI/BIO/35733/99
POCTI/BIO/35733/99
Investigador responsável
N/A
Concluído
2000 - 2002 Biotecnologia do gelano: via biossintetica, manipulação genética e ambiental e relações estrutura/funções
PRAXIS/P/BIO/12020/1998
PRAXIS/P/BIO/12020/1998
Investigador
N/A
1996 - 1999 Manipulação genética e ambiental de vias biossintéticas de exopolissacáridos bacterianos.
PRAXIS 2/2.1/BIO/1125/95
Investigador
1995 - 1998 Engenharia de células imobilizadas
PRAXIS 2/2.1/BIO/37/94
Bolseiro de Doutoramento
Concluído
1990 - 1993 Fisiologia de bactérias geneticamente manipuladas
BIO/913/90
BIO/913/90
Bolseiro de Doutoramento
N/A
Concluído
Produções

Publicações

Artigo em revista
  1. Celso Martins; Daryna Piontkivska; Dalila Mil-Homens; Paula Guedes; João M. P. Jorge; João Brinco; Cátia Bárria; et al. "Increased Production of Pathogenic, Airborne Fungal Spores upon Exposure of a Soil Mycobiota to Chlorinated Aromatic Hydrocarbon Pollutants". Microbiology Spectrum (2023): http://dx.doi.org/10.1128/spectrum.00667-23.
    10.1128/spectrum.00667-23
  2. Ricardo Estevens; Dalila Mil-Homens; Arsenio M. Fialho. "In-Silico Analysis Highlights the Existence in Members of Burkholderia cepacia Complex of a New Class of Adhesins Possessing Collagen-like Domains". Microorganisms (2023): https://doi.org/10.3390/microorganisms11051118.
    10.3390/microorganisms11051118
  3. Joana Souza-Gonçalves; Arsénio Fialho; Cleide M. F. Soares; Natália M. Osório; Suzana Ferreira-Dias. "Continuous Production of Dietetic Structured Lipids Using Crude Acidic Olive Pomace Oils". Molecules (2023): https://doi.org/10.3390/molecules28062637.
    10.3390/molecules28062637
  4. João F. Almeida; Matilde Marques; Vanessa Oliveira; Conceição Egas; Dalila Mil-Homens; Romeu Viana; Daniel F. R. Cleary; et al. "Marine Sponge and Octocoral-Associated Bacteria Show Versatile Secondary Metabolite Biosynthesis Potential and Antimicrobial Activities against Human Pathogens". Marine Drugs (2022): https://doi.org/10.3390/md21010034.
    10.3390/md21010034
  5. Sandra Godinho Silva; Patrícia Paula; José Paulo da Silva; Dalila Mil-Homens; Miguel Cacho Teixeira; Arsénio Mendes Fialho; Rodrigo Costa; Tina Keller-Costa. "Insights into the Antimicrobial Activities and Metabolomes of Aquimarina (Flavobacteriaceae, Bacteroidetes) Species from the Rare Marine Biosphere". Marine Drugs (2022): https://doi.org/10.3390/md20070423.
    10.3390/md20070423
  6. Cátia Bárria; Dalila Mil-Homens; Sandra N. Pinto; Arsénio M. Fialho; Cecília M. Arraiano; Susana Domingues. "RNase R, a New Virulence Determinant of Streptococcus pneumoniae". Microorganisms 10 2 (2022): 317-317. https://doi.org/10.3390/microorganisms10020317.
    10.3390/microorganisms10020317
  7. Rocha, Jaqueline; Ferreira, Catarina; Mil-Homens, Dalila; Busquets, Antonio; Fialho, Arsénio M.; Henriques, Isabel; Gomila, Margarita; Manaia, Célia M.. "Third generation cephalosporin-resistant Klebsiella pneumoniae thriving in patients and in wastewater: what do they have in common?". BMC Genomics 23 1 (2022): http://dx.doi.org/10.1186/s12864-021-08279-6.
    10.1186/s12864-021-08279-6
  8. Pinto, Sandra N.; Mil-Homens, Dalila; Pires, Rita F.; Alves, Marta M.; Serafim, Gabriel; Martinho, Nuno; Melo, Manuel; Fialho, Arsénio M.; Bonifácio, Vasco D. B.. "Core–shell polycationic polyurea pharmadendrimers: new-generation of sustainable broad-spectrum antibiotics and antifungals". Biomaterials Science 10 18 (2022): 5197-5207. http://dx.doi.org/10.1039/d2bm00679k.
    10.1039/d2bm00679k
  9. Araújo, Daniela; Mil-Homens, Dalila; Jørgensen, Per Trolle; Fialho, Arsénio M.; Wengel, Jesper; Henriques, Mariana; Silva, Sónia. "Exploration of anti EFG1 locked nucleic acid gapmers to control Candida albicans filamentation". Access Microbiology 3 12 (2021): http://dx.doi.org/10.1099/acmi.cc2021.po0058.
    10.1099/acmi.cc2021.po0058
  10. Ana Rita Garizo; Lígia F. Coelho; Sandra Pinto; Tiago P. Dias; Fábio Fernandes; Nuno Bernardes; Arsénio M. Fialho. "The Azurin-Derived Peptide CT-p19LC Exhibits Membrane-Active Properties and Induces Cancer Cell Death". Biomedicines 9 9 (2021): 1194-1194. https://doi.org/10.3390/biomedicines9091194.
    10.3390/biomedicines9091194
  11. Garizo, Ana Rita; Castro, Flávia; Martins, Cláudia; Almeida, Andreia; Dias, Tiago P.; Fernardes, Fábio; Barrias, Cristina C.; et al. "p28-functionalized PLGA nanoparticles loaded with gefitinib reduce tumor burden and metastases formation on lung cancer". Journal of Controlled Release 337 (2021): 329-342. http://dx.doi.org/10.1016/j.jconrel.2021.07.035.
    10.1016/j.jconrel.2021.07.035
  12. Andreia I. Pimenta; Michelle Kilcoyne; Nuno Bernardes; Dalila Mil-Homens; Lokesh Joshi; Arsenio M. Fialho. "Burkholderia cenocepacia BCAM2418-induced antibody inhibits bacterial adhesion, confers protection to infection and enables identification of host glycans as adhesin targets". Cellular Microbiology (2021): https://doi.org/10.1111/cmi.13340.
    10.1111/cmi.13340
  13. Cavalheiro, Mafalda; Pereira, Diana; Formosa-Dague, Cécile; Leitão, Carolina; Pais, Pedro; Ndlovu, Easter; Viana, Romeu; et al. "From the first touch to biofilm establishment by the human pathogen Candida glabrata: a genome-wide to nanoscale view". Communications Biology 4 1 (2021): http://dx.doi.org/10.1038/s42003-021-02412-7.
    10.1038/s42003-021-02412-7
  14. Pimenta, Andreia I.; Bernardes, Nuno; Alves, Marta M.; Mil-Homens, Dalila; Fialho, Arsenio M.. Autor correspondente: Fialho, Arsenio M.. "Burkholderia cenocepacia transcriptome during the early contacts with giant plasma membrane vesicles derived from live bronchial epithelial cells". Scientific Reports 11 1 (2021): http://dx.doi.org/10.1038/s41598-021-85222-5.
    Publicado • 10.1038/s41598-021-85222-5
  15. Cavalheiro, Mafalda; Romão, Daniela; Santos, Rui; Mil-Homens, Dalila; Pais, Pedro; Costa, Catarina; Galocha, Mónica; et al. "Role of CgTpo4 in Polyamine and Antimicrobial Peptide Resistance: Determining Virulence in Candida glabrata". International Journal of Molecular Sciences 22 3 (2021): 1376. http://dx.doi.org/10.3390/ijms22031376.
    10.3390/ijms22031376
  16. Pires, Diana P.; Monteiro, Rodrigo; Mil-Homens, Dalila; Fialho, Arsénio; Lu, Timothy K.; Azeredo, Joana. "Designing P. aeruginosa synthetic phages with reduced genomes". Scientific Reports 11 1 (2021): http://dx.doi.org/10.1038/s41598-021-81580-2.
    10.1038/s41598-021-81580-2
  17. Silva, Marília; Monteiro, Gabriel Amaro; Fialho, Arsenio M.; Bernardes, Nuno; da Silva, Cláudia Lobato. "Conditioned Medium From Azurin-Expressing Human Mesenchymal Stromal Cells Demonstrates Antitumor Activity Against Breast and Lung Cancer Cell Lines". Frontiers in Cell and Developmental Biology 8 (2020): http://dx.doi.org/10.3389/fcell.2020.00471.
    10.3389/fcell.2020.00471
  18. Santanu Sasidharan; Chandrabose Selvaraj; Sanjeev Kumar Singh; Vikash Kumar Dubey; Sachin Kumar; Arsenio M. Fialho; Prakash Saudagar. "Bacterial protein azurin and derived peptides as potential anti-SARS-CoV-2 agents: insights from molecular docking and molecular dynamics simulations". Journal of Biomolecular Structure and Dynamics (2020): https://doi.org/10.1080/07391102.2020.1787864.
    10.1080/07391102.2020.1787864
  19. Andreia I. Pimenta; Dalila Mil-Homens; Sandra N. Pinto; Arsenio M. Fialho. "Phenotypic characterization of trimeric autotransporter adhesin-defective bcaC mutant of Burkholderia cenocepacia: cross-talk towards the histidine kinase BCAM0218". Microbes and Infection (2020): https://doi.org/10.1016/j.micinf.2020.05.018.
    10.1016/j.micinf.2020.05.018
  20. Pimenta, Andreia I.; Mil-Homens, Dalila; Fialho, Arsenio M.. "Burkholderia cenocepacia –host cell contact controls the transcription activity of the trimeric autotransporter adhesin BCAM2418 gene". MicrobiologyOpen 9 4 (2020): http://dx.doi.org/10.1002/mbo3.998.
    10.1002/mbo3.998
  21. Pedro Pais; Susana Vagueiro; Dalila Mil-Homens; Andreia I. Pimenta; Romeu Viana; Michiyo Okamoto; Hiroji Chibana; Arsénio M. Fialho; Miguel C. Teixeira. "A new regulator in the crossroads of oxidative stress resistance and virulence in Candida glabrata: The transcription factor CgTog1". Virulence (2020): https://doi.org/10.1080/21505594.2020.1839231.
    10.1080/21505594.2020.1839231
  22. Oliveira, Hugo; Mendes, Ana; Fraga, Alexandra G.; Ferreira, Alice; Pimenta, Andreia I.; Mil-Homens, Dalila; Fialho, Arsénio M.; Pedrosa, Jorge; Azeredo, Joana. "K2 Capsule Depolymerase Is Highly Stable, Is Refractory to Resistance, and Protects Larvae and Mice from Acinetobacter baumannii Sepsis". Applied and Environmental Microbiology 85 17 (2019): http://dx.doi.org/10.1128/aem.00934-19.
    10.1128/aem.00934-19
  23. Nuno Bernardes; Arsenio M. Fialho. "Perturbing the Dynamics and Organization of Cell Membrane Components: A New Paradigm for Cancer-Targeted Therapies". International Journal of Molecular Sciences (2018): https://doi.org/10.3390/ijms19123871.
    10.3390/ijms19123871
  24. Cavalheiro, Mafalda; Costa, Catarina; Silva-Dias, Ana; Miranda, Isabel M.; Wang, Can; Pais, Pedro; Pinto, Sandra N.; et al. "A Transcriptomics Approach To Unveiling the Mechanisms ofIn VitroEvolution towards Fluconazole Resistance of aCandida glabrataClinical Isolate". Antimicrobial Agents and Chemotherapy 63 1 (2018): http://dx.doi.org/10.1128/aac.00995-18.
    10.1128/aac.00995-18
  25. Nuno Bernardes; Ana Rita Garizo; Sandra N. Pinto; Bernardo Caniço; Catarina Perdigão; Fábio Fernandes; Arsenio M. Fialho. "Azurin interaction with the lipid raft components ganglioside GM-1 and caveolin-1 increases membrane fluidity and sensitivity to anti-cancer drugs". Cell Cycle 17 13 (2018): 1649-1666. https://doi.org/10.1080/15384101.2018.1489178.
    10.1080/15384101.2018.1489178
  26. Mil-Homens, Dalila; Barahona, Susana; Moreira, Ricardo N; Silva, Ines J; Pinto, Sandra N; Fialho, Arsenio M; Arraiano, Cecilia M. "The stress-response protein BolA influences fitness and promotes Salmonella Typhimurium virulence.". Applied and environmental microbiology (2018): http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=MEDLINE&KeyUT=MEDLINE:29439986&KeyUID=MEDLINE:29439986.
    10.1128/AEM.02850-17
  27. Romão, D.; Cavalheiro, M.; Mil-Homens, D.; Santos, R.; Pais, P.; Costa, C.; Takahashi-Nakaguchi, A.; et al. "A New Determinant of Candida glabrata Virulence: The Acetate Exporter CgDtr1". Front Cell Infect Microbiol 7 (2017): 473-473. https://www.ncbi.nlm.nih.gov/pubmed/29184852.
    10.3389/fcimb.2017.00473
  28. Silva, L. O.; Nobre, L. S.; Mil-Homens, D.; Fialho, A.; Saraiva, L. M.. "Repair of Iron Centers RIC protein contributes to the virulence of Staphylococcus aureus". Virulence (2017): 0-0. https://www.ncbi.nlm.nih.gov/pubmed/29020514.
    10.1080/21505594.2017.1389829
  29. Santos, R.; Costa, C.; Mil-Homens, D.; Romão, D.; de Carvalho, C. C.; Pais, P.; Mira, N. P.; Fialho, A. M.; Teixeira, M. C.. "The multidrug resistance transporters CgTpo1_1 and CgTpo1_2 play a role in virulence and biofilm formation in the human pathogen Candida glabrata". Cell Microbiol (2016): https://www.ncbi.nlm.nih.gov/pubmed/27780306.
    10.1111/cmi.12686
  30. Mil-Homens, D.; Ferreira-Dias, S.; Fialho, A. M.. "Fish oils against Burkholderia and Pseudomonas aeruginosa: in vitro efficacy and their therapeutic and prophylactic effects on infected Galleria mellonella larvae". J Appl Microbiol (2016):
    10.1111/jam.13145
  31. Bernardes, N.; Abreu, S.; Carvalho, F. A.; Fernandes, F.; Santos, N. C.; Fialho, A. M.. "Modulation of membrane properties of lung cancer cells by azurin enhances the sensitivity to EGFR-targeted therapy and decreased ß1 integrin-mediated adhesion". Cell Cycle (2016): 0-0.
    10.1080/15384101.2016.1172147
  32. Moreira, A. S.; Mil-Homens, D.; Sousa, S. A.; Coutinho, C. P.; Pinto-de-Oliveira, A.; Ramos, C. G.; Dos Santos, S. C.; et al. "Variation of Burkholderia cenocepacia virulence potential during cystic fibrosis chronic lung infection". Virulence (2016): 0-0.
    10.1080/21505594.2016.1237334
  33. Mil-Homens, D.; Pinto, S. N.; Matos, R. G.; Arraiano, C.; Fialho, A. M.. "Burkholderia cenocepacia K56-2 trimeric autotransporter adhesin BcaA binds TNFR1 and contributes to induce airway inflammation". Cell Microbiol (2016):
    10.1111/cmi.12677
  34. Mil-Homens, Dalila; Leca, Maria Ines; Fernandes, Fabio; Pinto, Sandra N.; Fialho, Arsenio M.. "Characterization of BCAM0224, a Multifunctional Trimeric Autotransporter from the Human Pathogen Burkholderia cenocepacia". Journal of Bacteriology 196 11 (2014): 1968-1979.
    10.1128/jb.00061-14
  35. Bernardes, Nuno; Ribeiro, Ana Sofia; Abreu, Sofia; Vieira, Andre F.; Carreto, Laura; Santos, Manuel; Seruca, Raquel; Paredes, Joana; Fialho, Arsenio M.. "High-throughput molecular profiling of a P-cadherin overexpressing breast cancer model reveals new targets for the anti-cancer bacterial protein azurin". International Journal of Biochemistry & Cell Biology 50 (2014): 1-9.
    10.1016/j.biocel.2014.01.023
  36. Viegas, Sandra C.; Mil-Homens, Dalila; Fialho, Arsenio M.; Arraiano, Cecilia M.. "The Virulence of Salmonella enterica Serovar Typhimurium in the Insect Model Galleria mellonella Is Impaired by Mutations in RNase E and RNase III". Applied and Environmental Microbiology 79 19 (2013): 6124-6133. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000324176900037&KeyUID=WOS:000324176900037.
    10.1128/aem.02044-13
  37. El-Kirat-Chatel, S.; Mil-Homens, D.; Beaussart, A.; Fialho, A.M.; Dufrêne, Y.F.. "Single-molecule atomic force microscopy unravels the binding mechanism of a Burkholderia cenocepacia trimeric autotransporter adhesin". Molecular Microbiology 89 4 (2013): 649-659. http://www.scopus.com/inward/record.url?eid=2-s2.0-84881545645&partnerID=MN8TOARS.
    10.1111/mmi.12301
  38. Bernardes, N.; Ribeiro, A.S.; Abreu, S.; Mota, B.; Matos, R.G.; Arraiano, C.M.; Seruca, R.; Paredes, J.; Fialho, A.M.. "The Bacterial Protein Azurin Impairs Invasion and FAK/Src Signaling in P-Cadherin-Overexpressing Breast Cancer Cell Models". PLoS ONE 8 7 (2013): http://www.scopus.com/inward/record.url?eid=2-s2.0-84880423678&partnerID=MN8TOARS.
    10.1371/journal.pone.0069023
  39. Bernardes, N.; Chakrabarty, A.M.; Fialho, A.M.. "Engineering of bacterial strains and their products for cancer therapy". Applied Microbiology and Biotechnology 97 12 (2013): 5189-5199. http://www.scopus.com/inward/record.url?eid=2-s2.0-84878687925&partnerID=MN8TOARS.
    10.1007/s00253-013-4926-6
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  96. Richau, J.A.; Choquenet, D.; Fialho, A.M.; Moreira, L.M.; Sá-Correia, I.. "The biosynthesis of the exopolysaccharide gellan results in the decrease of Sphingomonas paucimobilis tolerance to copper". Enzyme and Microbial Technology 20 7 (1997): 510-515. http://www.scopus.com/inward/record.url?eid=2-s2.0-0031149975&partnerID=MN8TOARS.
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  97. Richau, J.A.; Choquenet, D.; Fialho, A.M.; Sá-Correia, I.. "Emergence of Cu++-tolerant mutants defective in gellan synthesis in Cu++-stressed cultures of sphingomonas paucimobilis". Research in Microbiology 148 3 (1997): 251-261. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030935689&partnerID=MN8TOARS.
    10.1016/S0923-2508(97)85245-X
  98. Santana, FMC; Fialho, AM; SaCorreia, I; Empis, JMA. "Isolation of bacterial strains capable of using lupanine, the predominant quinolizidine alkaloid in white lupin, as sole carbon and energy source". Journal of Industrial Microbiology 17 2 (1996): 110-115.
    10.1007/BF01570053
  99. Leitao, J.H.; Fialho, A.M.; Sa-Correia, I.. "Effects of growth temperature on alginate synthesis and enzymes in Pseudomonas aeruginosa variants". Journal of General Microbiology 138 3 (1992): 605-610. http://www.scopus.com/inward/record.url?eid=2-s2.0-0026551642&partnerID=MN8TOARS.
  100. Monteiro, G.A.; Fialho, A.M.; Ripley, S.J.; Sa-Correia, I.. "Electrotransformation of gellan-gum producing and non-producing Pseudomonas elodea strains". Journal of Applied Bacteriology 72 5 (1992): 423-428. http://www.scopus.com/inward/record.url?eid=2-s2.0-0026537272&partnerID=MN8TOARS.
  101. MONTEIRO, GA; FIALHO, AM; RIPLEY, SJ; SACORREIA, I. "ELECTROTRANSFORMATION OF GELLAN-GUM PRODUCING AND NONPRODUCING PSEUDOMONAS-ELODEA STRAINS". Journal of Applied Bacteriology 72 5 (1992): 423-428.
    10.1111/j.1365-2672.1992.tb01855.x
  102. Shinabarger, D.; Berry, A.; May, T.B.; Rothmel, R.; Fialho, A.; Chakrabarty, A.M.. "Purification and characterization of phosphomannose isomerase-guanosine diphospho-D-mannose pyrophosphorylase: A bifunctional enzyme in the alginate biosynthetic pathway of Pseudomonas aeruginosa". Journal of Biological Chemistry 266 4 (1991): 2080-2088. http://www.scopus.com/inward/record.url?eid=2-s2.0-0025871067&partnerID=MN8TOARS.
  103. Fialho, A.M.; Monteiro, G.A.; Sa-Correia, I.. "Conjugal transfer of recombinant plasmids into gellan gum-producing and non-producing variants of Pseudomonas elodea ATCC 31461". Letters in Applied Microbiology 12 3 (1991): 85-87. http://www.scopus.com/inward/record.url?eid=2-s2.0-0025971191&partnerID=MN8TOARS.
  104. Fialho, A.M.; Zielinski, N.A.; Fett, W.F.; Chakrabarty, A.M.; Berry, A.. "Distribution of alginate gene sequences in the Pseudomonas rRNA homology group I-Azomonas-Azotobacter lineage of superfamily B procaryotes". Applied and Environmental Microbiology 56 2 (1990): 436-443. http://www.scopus.com/inward/record.url?eid=2-s2.0-0025093589&partnerID=MN8TOARS.
  105. Fernandes, H.L.; Tomé, M.M.; Lupi, F.M.; Fialho, A.M.; Sá-Correia, I.; Novais, J.M.. "Biosynthesis of high concentrations of an exopolysaccharide during the cultivation of the microalga Botryococcus braunii". Biotechnology Letters 11 6 (1989): 433-436. http://www.scopus.com/inward/record.url?eid=2-s2.0-0001224751&partnerID=MN8TOARS.
    10.1007/BF01089478
Capítulo de livro
  1. Bernardes, Nuno; Fialho, Arsénio. "Overcoming the Plasma Membrane". In Organelle and Molecular Targeting. CRC Press, 2021.
    Publicado • 10.1201/9781003092773
  2. Garizo, Ana Rita; Bernardes, Nuno; Chakrabarty, Ananda M.; Fialho, Arsénio M.. "The Anticancer Potential of the Bacterial Protein Azurin and Its Derived Peptide p28". In Microbial Infections and Cancer Therapy, 319-338. Jenny Stanford Publishing, 2019.
    10.1201/9781351041904-9
  3. Coelho, Lígia F.; Bernardes, Nuno; Fialho, Arsénio M.. "Prospective Therapeutic Applications of Bacteriocins as Anticancer Agents". In Microbial Infections and Cancer Therapy, 339-366. Jenny Stanford Publishing, 2019.
    10.1201/9781351041904-10
  4. Fialho, Arsénio. "Bioremediation strategies with Pseudomonas sp. ADP in worst-case scenarios of soil contamination with chlorinated s-triazines". In Industrial, medical and environmental applications of microorganisms. Wageningen Academic Publishers, 2014.
  5. Fialho, Arsénio. "Bioremediation of soils contaminated with atrazine and other s-triazine herbicides: current state and prospects". In Advances in Environmental Research, 1-49. 2012.
    Publicado
  6. Fialho,, Arsenio M.; Chakrabarty, Ananda M.. "Recent Patents on Bacterial Proteins as Potential Anticancer Agents". In Frontiers in Anti-Cancer Drug Discovery, 601-622. BENTHAM SCIENCE PUBLISHERS, 2011.
    10.2174/978160805161811001010601
  7. Fialho, A.M.; Chakrabarty, A.M.. "The Role and Importance of Intellectual Property Generation and Protection in Drug Development". In Emerging Cancer Therapy: Microbial Approaches and Biotechnological Tools. 2010.
    Publicado
  8. Fialho, Arsénio. "Promiscuous Anticancer Drugs from Pathogenic Bacteria: Rational versus Intelligent Drug Design". In Emerging Cancer Therapy. Wiley, 2010.
  9. Fialho, Arsénio. "The Role and Importance of Intellectual Property Generation and Protection in Drug Development". In Emerging Cancer Therapy. Wiley, 2010.
  10. Fialho, Arsénio. "Host-Pathogen Interactions: From Infection to Drug Discovery". In Text Book on Molecular Biotechnology. 2009.
    Publicado
  11. Fialho, Arsénio. "Promiscuous Drugs from Pathogenic Bacteria in the Post-Antibiotics Era". In Patho-Biotechnology, 167-184. CRC Press, 2008.
    10.1201/9781498713740-20
  12. Fialho, Arsénio. "Ribonucleases e controlo pós-transcricional da expressão génica". In O Mundo do RNA: Novos Desafios e Perspectivas Futuras. Portugal: LIDEL, 2007.
    Publicado
  13. Fialho, Arsénio M.; Moreira, Leonilde M.; Granja, Ana Teresa; Hoffmann, Karen; Popescu, Alma; Sá-Correia, Isabel. "Biotechnology of the Bacterial Gellan Gum: Genes and Enzymes of the Biosynthetic Pathway". In A Portrait of State-of-the-Art Research at the Technical University of Lisbon, 233-250. Springer Netherlands, 2007.
    10.1007/978-1-4020-5690-1_15
  14. Fialho, Arsénio. "Engenharia Genética". In Biotecnologia- Fundamentos e Aplicações. 2003.
    Publicado
  15. Fialho, Arsénio. "Electrotransformation of Sphingomonas paucimobilis". In Electrotransformation of Bacteria. Springer Berlin Heidelberg, 2000.
Edição de livro
  1. Fialho, Arsénio. Microbial Infections and Cancer Therapy. Jenny Stanford Publishing. 2019.
    10.1201/9781351041904
  2. Fialho, Arsénio. Autor correspondente: Fialho, Arsénio. Emerging Cancer Therapy. Wiley. 2010.
    Publicado • 10.1002/9780470626528 • Editor
  3. Fialho, Arsénio. O Mundo do RNA: Novos Desafios e Perspectivas Futuras. Portugal: Lidel. 2007.
    Publicado • Editor

Propriedade Intelectual

Patente
  1. A Chakrabarty, TD Gupta, T Yamada, A Chaudhari, A Fialho, Y Zhu. 2021. "Compositions and methods for treating conditions related to ephrin signaling with Cupredoxins".
  2. Fialho, Arsénio. 2019. "Cupredoxin derived transport agents and methods of use thereof". Estados Unidos.
  3. Fialho, Arsénio. 2018. "Compositions and methods for treating conditions related to ephrin signaling with cupredoxins". Estados Unidos.
  4. Fialho, Arsénio. 2018. "Transport agents for crossing the blood-brain barrier and into brain cancer cells, and methods of use thereof".
  5. Fialho, Arsénio. 2015. "Compositions and methods for treating conditions related to ephrin signaling with cupredoxins and mutants thereof". Estados Unidos.
  6. Fialho, Arsénio. 2014. "Compositions and methods for treating malaria with cupredoxin and cytochrome".
  7. Fialho, Arsénio. 2010. "Compositions and methods to prevent and/or treat cancer with Pa-CARD". Estados Unidos.
  8. Fialho, Arsénio. 2009. "Compositions and methods for treating HIV infection with cupredoxin and cytochrome c". Estados Unidos.
  9. Fialho, Arsénio. 2008. "Compositions and methods for treating conditions related to ephrin signaling with cupredoxins". Estados Unidos.
Atividades

Orientação

Título / Tema
Papel desempenhado
Curso (Tipo)
Instituição / Organização
2024 - Atual Evaluating glycans of eukaryotic origin as antagonists of bacterial adhesion
Orientador
Biotecnologia e Biociências (Doutoramento)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2019 - Atual p53/miR-181d/DEPTOR, a novel tumor-suppressor axis for regulation of growth and metastasis in breast cancer
Coorientador
Biotecnologia e Biociências (Doutoramento)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2019 - Atual Deciphering the evolutionary trajectory of Burkholderia multivorans during chronic respiratory infections in cystic fibrosis patients and unraveling the mechanisms of multicellular aggregates formation
Coorientador
Biotecnologia e Biociências (Doutoramento)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2018 - 2021 p28 peptide-functionalized gefitinib-loaded PLGA nanoparticles: development, characterization and in vitro evaluation of their anti-lung cancer activity
Orientador
Biotecnologia e Biociências (Doutoramento)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2017 - 2021 Chronic Haemolysis Pathophysiology in Sickle Cell Disease
Coorientador
Biotecnologia e Biociências (Doutoramento)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2017 - 2020 Trimeric Autotransporter Adhesins in the opportunistic pathogen Burkholderia cenocepacia: advances in the understanding of their mechanisms of action
Orientador
Biotecnologia e Biociências (Doutoramento)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2009 - 2013 Bacterial protein azurin as a new therapeutic tool to treat poor prognosis breast cancer with overexpression of P-cadherin
Orientador
Biotecnologia e Biociências (Doutoramento)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2008 - 2012 Burkholderia cenocepacia pathogenesis: unravelling the virulence functions of trimeric autotransporter adhesins and development of alternative therapies to target this pathogen
Orientador
Biotecnologia e Biociências (Doutoramento)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2004 - 2008 Gellan gum biosynthesis in Sphingomonas elodea and cytotoxicity studies of redox proteins: characterization of UDP-glucose dehydrogenase, a key enzyme of gellan biosynthesis and evaluation of gellan as a stabilizer of protein and peptide-based anticancer agents
Orientador
Biotecnologia e Biociências (Doutoramento)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2000 - 2004 Estratégias para acelerar a descontaminação de solos poluídos com o herbicida atrazina: inoculação da bactéria mineralizadora de atrazina Pseudomonas sp ADP, bioestimulação por adição de citrato ou succinato e biossíntese e adição de emulsionantes
Orientador
Biotecnologia e Biociências (Doutoramento)
Universidade de Lisboa Instituto Superior Técnico, Portugal
1999 - 2003 Biotecnologia do polissacárido gelano produzido por Sphingomonas paucimobilis: genes envolvidos na sua síntese e manipulação genética e ambiental da via biossintética
Coorientador
Biotecnologia e Biociências (Doutoramento)
Universidade de Lisboa Instituto Superior Técnico, Portugal
1998 - 2002 Biossíntese do exopolissacárido gelano em Sphingomonas paucimobilis ATCC 31461: genes, enzimas e manipulação genética da via biossintética
Coorientador
Biotecnologia e Biociências (Doutoramento)
Universidade de Lisboa Instituto Superior Técnico, Portugal

Comissão de avaliação

Descrição da atividade
Tipo de assessoria
Instituição / Organização Entidade financiadora
2020 - 2024 National Science Centre, Poland (Expert Review Panel member).
Avaliador
National Science Centre, Poland
2020 - 2024 EU expert number: EX2017D302781
Avaliador
Agence Europe SA
Distinções

Prémio

2019 Pedagogical Innovation Project (PIP) sponsored by Pedagogical Council of IST, 2019/20
Universidade de Lisboa Instituto Superior Técnico, Portugal
2019 IST Teaching Awards
Universidade de Lisboa Instituto Superior Técnico, Portugal
2014 IST Teaching Awards
Universidade de Lisboa Instituto Superior Técnico, Portugal
2014 Prémio de mérito – cidade de Moura
Municipality of Moura, Portugal
2011 IST Teaching Awards
Universidade de Lisboa Instituto Superior Técnico, Portugal
2006 IST Teaching Awards
Universidade de Lisboa Instituto Superior Técnico, Portugal

Título

2005 Invited Associate Professor
University of Illinois at Chicago, Estados Unidos