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PhD student in Bioengineering, Cell Therapies and Regenerative Medicine from April of 2021, at the Stem Cell Engineering Research Group (SCERG), Institute for Bioengineering and Biosciences (iBB), Instituto Superior Técnico (IST). Her research is focused on the establishment of a manufacturing platform able to reach clinically meaningful numbers of umbilical cord blood (UCB) chimeric antigen receptor-natural killer (CAR-NK) cells and derived extracellular vesicles (EVs), using defined good manufacturing pracices (GMP)-compliant reagents, for immunotherapy. She graduated in Biological Engineering from IST in November 2019. During her studies, she developed a special interest for stem cells, which led her to complete her master's thesis in the Stem Cell Institute Leuven at KU Leuven, with the topic "Co-Culture Strategies Mimicking the Fetal Liver Niche for In Vitro Expansion of Hematopoietic Stem Cells". The skills obtained until now include cell culture techniques, analytical cell monitoring techniques, with emphasis on FACS, and molecular biology techniques, among others. During her master's thesis , she had the oportunity to work with animal models, in what concerns bone marrow extraction, and to perform co-culture experiments. Other personal competencies developed include organization, spirit of initiative, autonomy and teamwork.
Identificação

Identificação pessoal

Nome completo
Isabel Maria Pereira Doutor

Nomes de citação

  • Doutor, Isabel

Identificadores de autor

Ciência ID
5E1E-ED6C-6AD7
ORCID iD
0000-0002-2807-6619

Endereços de correio eletrónico

  • isabel.doutor@tecnico.ulisboa.pt (Profissional)

Websites

Domínios de atuação

  • Ciências Naturais - Ciências Biológicas
  • Ciências da Engenharia e Tecnologias - Engenharia Química - Engenharia dos Processos Químicos
  • Ciências Médicas e da Saúde - Biotecnologia Médica - Biotecnologia Aplicada à Saúde
  • Ciências Médicas e da Saúde - Medicina Básica - Imunologia

Idiomas

Idioma Conversação Leitura Escrita Compreensão Peer-review
Português (Idioma materno)
Inglês Utilizador independente (B2) Utilizador proficiente (C1) Utilizador independente (B2) Utilizador independente (B2) Utilizador independente (B2)
Espanhol; Castelhano Utilizador independente (B1) Utilizador independente (B2) Utilizador independente (B1) Utilizador independente (B1) Utilizador independente (B1)
Francês Utilizador independente (B1) Utilizador independente (B2) Utilizador independente (B1) Utilizador independente (B1) Utilizador independente (B1)
Holandês Utilizador elementar (A1) Utilizador elementar (A2) Utilizador elementar (A1) Utilizador elementar (A1) Utilizador elementar (A1)
Formação
Grau Classificação
2021/04 - 2025
Em curso
Bioengenharia - Terapias Celulares e Medicina Regenerativa (Doutoramento)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2014/09 - 2019/11/18
Concluído
Engenharia Biológica (Mestrado integrado)
Universidade de Lisboa Instituto Superior Técnico, Portugal
"Co-Culture Strategies Mimicking the Fetal Liver Niche for In Vitro Expansion of Hematopoietic Stem Cells" (TESE/DISSERTAÇÃO)
17
2019/02/10 - 2019/07/10
Concluído
Engenharia Biológica (Master)
Katholieke Universiteit Leuven Faculteit Geneeskunde, Bélgica
"Co-Culture Strategies Mimicking the Fetal Liver Niche for In Vitro Expansion of Hematopoietic Stem Cells" (TESE/DISSERTAÇÃO)
18
Percurso profissional

Ciência

Categoria Profissional
Instituição de acolhimento
Empregador
2021/04/05 - 2025/04/05 Investigador (Investigação) Universidade de Lisboa Instituto Superior Técnico, Portugal
Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal

Outros

Categoria Profissional
Instituição de acolhimento
Empregador
2019/02/10 - 2019/07/10 ERASMUS Student Researcher, "Co-Culture Strategies Mimicking the Fetal Liver Niche for In Vitro Expansion of Hematopoietic Stem Cells" (Master Thesis) Katholieke Universiteit Leuven Faculteit Geneeskunde, Bélgica
Katholieke Universiteit Leuven Faculteit Geneeskunde, Bélgica
(...)
2018/07 - 2018/09 Student Researcher, "Biological Activity Study of Cynara cardunculus Leaves Extracts: In Vitro Cell Assays" Centro de Biotecnologia Agrícola e Agro-Alimentar do Alentejo, Portugal
Centro de Biotecnologia Agrícola e Agro-Alimentar do Alentejo, Portugal
2017/09 - 2018/07 Student Researcher, "Bioengineering Strategies for Pluripotent Stem Cell Expansion and Differentiation" Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
(...)
2017/07/03 - 2017/09/15 Student Researcher, "Characterization and Selective Fractionation of Relevant Lignocellulosic Materials from the Alentejo Region within the Biorefinaria Concept" Centro de Biotecnologia Agrícola e Agro-Alimentar do Alentejo, Portugal
Centro de Biotecnologia Agrícola e Agro-Alimentar do Alentejo, Portugal
Projetos

Bolsa

Designação Financiadores
2021/04/05 - 2025/04/04 Technology-driven platform for the manufacturing of extracellular vesicles derived from umbilical cord blood CARengineered NK cells for immunotherapy
UI/BD/151248/2021
Bolseiro de Doutoramento
Fundação para a Ciência e a Tecnologia
Em curso
2022 - 2024 NKBoost - Quality by design-centered platform for the manufacturing of CAR-engineered NK cells and extracellular vesicles for immunotherapy
PTDC/EQU-EQU/3708/2021
Bolseiro de Integração na Investigação
Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal

Universidade de Lisboa Instituto Superior Técnico, Portugal
Fundação para a Ciência e a Tecnologia
Em curso

Outro

Designação Financiadores
2018/07 - 2018/09 ValBioTecCynara - Valorização económica do cardo (Cynara cardunculus): variabilidade natural e suas aplicações biotecnológicas
ALT20-03-0145- FEDER-000038
Outra
Centro de Biotecnologia Agrícola e Agro-Alimentar do Alentejo, Portugal
Em curso
2017/07 - 2017/09 SelecTEcoli - Seleção e caraterização de estirpes de E. coli com tolerância acrescida a multi-inibidores derivados dos processos de pré-tratamento da biomassa lenhocelulósica
ALT20-03-0145-FEDER-000034
Outra
Centro de Biotecnologia Agrícola e Agro-Alimentar do Alentejo, Portugal
Em curso
Produções

Publicações

Artigo em conferência
  1. Ivone Torrado; Isabel Doutor; Florbela Carvalheiro; Maria da Conceição Fernandes; Helena Pereira; Luís C. Duarte. "Microwave Assisted Hydrothermal Pre-Treatment of Corn Cobs for Xylooligosaccharides Production". Trabalho apresentado em 4th Iberoamerican Congress on Biorefineries, Jaén, 2018.
Poster em conferência
  1. Gabriel Costa; Doutor, Isabel; Ana Fernandes-Platzgummer. "Influence of different culture media exchange ratios on umbilical cord blood-derived Natural Killer cell expansion". Trabalho apresentado em 28th ESACT Meeting, 2024.
  2. Doutor, Isabel; Ulpiano, Cristiana; Costa, Gabriel; Ana Azevedo; Fernandes-Platzgummer, Ana; Cláudia Lobato da Silva. "Harnessing the power of cord blood natural killer cells for cancer immunotherapy through cytotoxic extracellular vesicles". Trabalho apresentado em XLIX Annual Meeting of the Portuguese Society for Immunology, 2024.
  3. Gabriel Costa; Doutor, Isabel; Ana Fernandes-Platzgummer. "Influence of different culture media exchange ratios on NK cell expansion". Trabalho apresentado em XLIX Annual Meeting of the Portuguese Society for Immunology, 2024.
  4. Doutor, Isabel; Ulpiano, Cristiana; Cláudia Alexandra Martins Lobato da Silva; Ana Azevedo; Fernandes-Platzgummer, Ana. "Development of a manufacturing platform for umbilical cord blood natural killer cells and derived extracellular vesicles for immunotherapy". Trabalho apresentado em 7ª Conferência Anual da RedeSaúde, 2023.
  5. Gabriel Costa; Doutor, Isabel; Ana Fernandes-Platzgummer. "Influence of different culture media exchange ratios on NK cell expansion". Trabalho apresentado em III iBB Workshop, 2023.
  6. Doutor, Isabel; Ulpiano, Cristiana; Cláudia Alexandra Martins Lobato da Silva; Ana Azevedo; Fernandes-Platzgummer, Ana. "Development of a manufacturing platform for umbilical cord blood natural killer cells and derived extracellular vesicles for immunotherapy". Trabalho apresentado em III iBB Workshop, 2023.
  7. Doutor, Isabel; Ulpiano, Cristiana; Cláudia Alexandra Martins Lobato da Silva; Ana Azevedo; Fernandes-Platzgummer, Ana. "Development of a manufacturing platform for umbilical cord blood natural killer cells and derived extracellular vesicles for immunotherapy". Trabalho apresentado em ESGCT 30th Annual Congress, 2023.
  8. Doutor, Isabel; Branco, André; Ulpiano, Cristiana; Cláudia Alexandra Martins Lobato da Silva; Fernandes-Platzgummer, Ana. "Ex vivo expansion of umbilical cord blood-derived natural killer cells under static conditions and production of derived extracellular vesicles". Trabalho apresentado em International Society for Cell & Gene Therapy 2023 Meeting, 2023.
  9. Doutor, Isabel; Branco, André; Ulpiano, Cristiana; Cláudia Alexandra Martins Lobato da Silva; Fernandes-Platzgummer, Ana. "Optimizing the ex vivo expansion of umbilical cord blood natural killer cells and derived extracellular vesicle production under static conditions". Trabalho apresentado em XLVIII Annual Meeting Portuguese Society for Immunology, 2023.
  10. Doutor, Isabel; Branco, André; Cláudia Alexandra Martins Lobato da Silva; Fernandes-Platzgummer, Ana. "Optimization of the Ex Vivo Expansion of Umbilical Cord Blood Natural Killer Cells and Production of Derived Extracellular Vesicles Under Static Conditions". Trabalho apresentado em 33rd Annual Meeting ESACT-UK 2023, 2023.
  11. Doutor, Isabel. "Technology-driven platform for the manufacturing of extracellular vesicles derived from umbilical cord blood CAR- engineered NK cells for immunotherapy". Trabalho apresentado em PhD Open Days, 2022.
  12. Doutor, Isabel; Branco, André; Cláudia Alexandra Martins Lobato da Silva; Fernandes-Platzgummer, Ana. "Optimization of ex vivo umbilical cord blood-derived natural killer (NK) cell expansion under static conditions envisaging the standardized production of NK-derived extracellular vesicles". Trabalho apresentado em 2nd iBB Workshop, 2022.
  13. Doutor, Isabel; Branco, André; Cláudia Alexandra Martins Lobato da Silva; Fernandes-Platzgummer, Ana. "Optimization of ex vivo umbilical cord blood-derived natural killer cell expansion and production of derived extracellular vesicles under static conditions". Trabalho apresentado em 27th ESACT Meeting of the European Society for Animal Cell Technology, 2022.
  14. Doutor, Isabel; Branco, André; Cláudia Alexandra Martins Lobato da Silva; Fernandes-Platzgummer, Ana. "Optimization of the ex-vivo expansion of umbilical cord blood-derived natural killer cells and production of their derived extracellular vesicles under static conditions". Trabalho apresentado em 12th International Meeting of the Portuguese Society for Stem Cells and Cell Therapies, 2022.
  15. Ivone Torrado; Isabel Doutor; Florbela Carvalheiro; Maria da Conceição Fernandes; Helena Pereira; Luís C. Duarte. "Microwave Assisted Hydrothermal Pre-Treatment of Corn Cobs for Xylooligosaccharides Production". Trabalho apresentado em 4th Iberoamerican Congress on Biorefineries, 2018.
Tese / Dissertação
  1. "Co-Culture Strategies Mimicking the Fetal Liver Niche for In Vitro Expansion of Hematopoietic Stem Cells". Mestrado, Universidade de Lisboa Instituto Superior Técnico, 2019. https://scholar.tecnico.ulisboa.pt/api/records/IsXq2ToRnsN1NEyVOlReD44Agpd0uR3m1C7D/file/2e14e6e7d9392169b4c69353d145d6fc37380bd95fbfa0a8f88c2885fe4d31f8.pdf.
Atividades

Apresentação oral de trabalho

Título da apresentação Nome do evento
Anfitrião (Local do evento)
2024/06 Cord blood natural killer cells: a promising source of cytotoxic extracellular vesicles for cancer immunotherapy 28th ESACT Meeting
(Edinburgh, Reino Unido)
2023/11/14 Development of a manufacturing platform for umbilical cord blood natural killer cells and derived extracellular vesicles for immunotherapy 7ª Conferência Anual da RedeSaúde
(Lisbon, Portugal)
2023/11/13 Umbilical cord blood natural killer cells and derived extracellular vesicles manufacturing platform for immunotherapy III iBB Workshop
(Lisbon, Portugal)
2023/07/14 Umbilical Cord Blood Natural Killer Cells and Derived Extracellular Vesicles for Immunotherapy: Optimizing Cell Expansion and EV Production and Isolation SNEV Virtual Conference
Student Network on Extracellular Vesicles
2023/06/02 Impact of culture media on the expansion and functionality of umbilical cord blood natural killer cells and derived extracellular vesicles 2nd Meeting of the Portuguese Network on Extracellular Vesicles
Portuguese Network on Extracellular Vesicles (Lisbon, Portugal)
2023/01/11 Optimization of the Ex Vivo Expansion of Umbilical Cord Blood Natural Killer Cells and Production of Derived Extracellular Vesicles Under Static Conditions 33rd Annual Meeting ESACT-UK 2023
ESACT-UK (Nottingham, Reino Unido)
2022/11/15 Technology-driven manufacturing platform of EVs derived from UCB CAR-engineered NK cells for immunotherapy PhD Open Days
Instituto Superior Técnico (Lisbon, Portugal)

Participação em evento

Descrição da atividade
Tipo de evento
Nome do evento
Instituição / Organização
2023/11/14 - Atual 7ª Conferência Anual da RedeSaúde: "Viver em Qualidade: Valor em Saúde e Bem-Estar" 7ª Conferência Anual da RedeSaúde
2023/11/13 - Atual III iBB Workshop
Conferência
III iBB Workshop
Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
2023/11/11 - Atual IST-Taguspark Open Day: We had the chance to show the facilities of the stem cell engineering and regenerative medicine laboratory to a diverse group of enthusiasts of all ages and share the potential of stem cell research with aspiring young scientists and curious minds.
Outro
IST-Taguspark Open Day
2023/06/26 - Atual GEIVEX Workshop: Clinical Translation Of EV-Based Therapeutic Products: This workshop focused on giving a first grasp of the steps needed for clinical translation for EV-based products. The sessions included GMP basics and requirements to regulatory issues and the industry viewpoint, given by experts in the field of cell and gene therapy, clinical and regulatory bodies.
Oficina (workshop)
GEIVEX Workshop: Clinical Translation Of EV-Based Therapeutic Products
2022/09/23 - Atual II iBB Workshop
Conferência
II iBB Workshop
2024/09/18 - 2024/09/19 CIMT Cancer Cell Therapy Summit
Conferência
Fundação Champalimaud, Portugal
2024/06/23 - 2024/06/26 28th ESACT Meeting
Encontro
28th European Society for Animal Cell Technology Meeting
2024/03/10 - 2024/03/15 Tiny Packages with Big Impact: Workshop on EVs Separation and Characterization
Oficina (workshop)
Tiny Packages with Big Impact: Workshop on EVs Separation and Characterization
University of Virginia School of Medicine, Estados Unidos
2023/10/24 - 2023/10/27 ESGCT 30th Annual Congress
Congresso
ESGCT 30th Annual Congress
Europen Society of Gene & Cell Therapy, Reino Unido
2023/07/13 - 2023/07/14 SNEV Virtual Conference
Conferência
SNEV Virtual Conference
2023/06/22 - 2023/06/23 2nd Meeting of the Portuguese Network on Extracellular Vesicles
Encontro
2nd Meeting of the Portuguese Network on Extracellular Vesicles
Universidade Nova de Lisboa Nova Medical School, Portugal
2023/05/24 - 2023/05/25 Flow Cytometry Facility User Training
Outro
Flow Cytometry Facility User Training
2023/04/26 - 2023/04/28 Training in Science Communication: An additional stimulus to develop training in science communication, as well as fostering dialogue between science and society (https://www.ulisboa.pt/evento/formacao-em-comunicacao-de-ciencia).
Outro
Training in Science Communication
Universidade de Lisboa, Portugal
2023/03/29 - 2023/03/31 XLVIII Annual Meeting of the Portuguese Society for Immunology
Encontro
XLVIII Annual Meeting of the Portuguese Society for Immunology
2023/01/11 - 2023/01/12 33rd Annual Meeting ESACT-UK 2023
Conferência
33rd Annual Meeting ESACT-UK 2023
2022/11/14 - 2022/11/16 PhD Open Days: An opportunity for more than one thousand PhD students to socialize and share their research with the rest of the academic community, companies, and alumni.
Encontro
PhD Open Days
Universidade de Lisboa Instituto Superior Técnico, Portugal
2022/07/11 - 2022/07/15 Advanced Therapies Summer Course: The aim of this course was the diffusion of knowledge about Advanced Therapies (gene therapy, somatic cell therapy, tissue engineering), and their therapeutic possibilities, with a special emphasis on their current applications in the field of genetic and neoplastic diseases as well as regenerative medicine.
Outro
Advanced Therapies Summer Course
Universidad de Murcia, Espanha
2022/06/26 - 2022/06/29 27th ESACT Meeting of the European Society for Animal Cell Technology
Conferência
27th ESACT Meeting of the European Society for Animal Cell Technology
2022/03/10 - 2022/03/12 12th International Meeting of the Portuguese Society for Stem Cells and Cell Therapies
Encontro
12th International Meeting of the Portuguese Society for Stem Cells and Cell Therapies
2019/03 - 2019/03 Flow Cytometry Facility User Training: This training is composed of a theory and practical instrument training. The theory covers the basic principles of flow cytometry; instrument components, fluorescence absorption and emission, compensation, experimental design and appropriate controls for flow cytometry. It also covers more advanced concepts such as multiparameter experimental design. In the instrument training, users practice using the instrument by running their own stained samples.
Outro
Flow Cytometry Facility User Training
Katholieke Universiteit Leuven Groep Biomedische Wetenschappen, Bélgica
2018/03/05 - 2018/03/09 IV Semana da Bioengenharia
Encontro
IV Semana da Bioengenharia
Universidade de Lisboa Instituto Superior Técnico, Portugal
2017/03/06 - 2017/03/10 III Semana da Bioengenharia
Encontro
III Semana da Bioengenharia
Universidade de Lisboa Instituto Superior Técnico, Portugal
2016/02/29 - 2016/03/04 II Semana da Bioengenharia (SBE): SBE happens every year at IST and used as a way of spreading both knowledge about what is done is the bioengineering area and the opportunities of investigation in Portugal and in the world, not only to students of the referred masters, but to all interested in this topic. This way the SBE is a week full of lectures from investigators and companies in the bioengineering area, scientific field trips to relevant investigation or industrial facilities, workshops and international guests, among others.
Encontro
II Semana da Bioengenharia
Universidade de Lisboa Instituto Superior Técnico, Portugal

Curso / Disciplina lecionado

Disciplina Curso (Tipo) Instituição / Organização
2022/11 - 2022/12 After School - Engenharia Genética e Bioprocessos 2022-02 (Ensino secundário)

Membro de associação

Nome da associação Tipo de participação
2016 - 2018 Núcleo de Engenharia Biológica Colaboradora (2016); Coordenadora (2017); Presidente do Conselho Fiscal (2018)

Tutoria

Tópico Nome do aluno
2023/09 - 2024/06 UCB-NK cell isolation and expansion and NK-EVs production and isolation Beatriz Filipe
2023/05 - 2024/02 UCB-NK cell isolation and expansion Gabriel Costa
Distinções

Prémio

2023 Best Oral Presentation on the SNEV Virtual Conference

Outra distinção

2019 Aluna de Mérito
Universidade de Lisboa Instituto Superior Técnico, Portugal
2018 Aluna de Mérito
Universidade de Lisboa Instituto Superior Técnico, Portugal
2017 Aluna de Mérito
Universidade de Lisboa Instituto Superior Técnico, Portugal
2016 Aluna de Mérito
Universidade de Lisboa Instituto Superior Técnico, Portugal
2015 Aluna de Mérito
Universidade de Lisboa Instituto Superior Técnico, Portugal