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Ângela Sousa obtained her degree in Biochemistry in 2007 from the University of Beira Interior (UBI), Portugal. In 2008 she was awarded an FCT PhD grant and spent 3 month-stay (2009) in the Department of Pure and Applied Biochemistry at Lund University, Sweden, to explore innovative chromatographic columns developed by GE Healthcare company. In 2011, she had a 1 month-stay in BIA Separations company, Slovenia, for chemistry immobilization of amino acid ligands in monolithic chromatographic supports, and in the end of that year, she received her PhD degree in Biochemistry from UBI (Portugal). In 2012 and 2015 she was awarded with two postdoctoral grants at the Health Sciences Research Centre of UBI (CICS-UBI) in Portugal. From February 2017 to August 2018, she was Invited Assistant Professor in the Sciences Faculty of UBI. From September 2018 to March 2023, she was a contract as Researcher (DL-57) from CICS-UBI in Health Biotechnology area. In 2019, she got a Guest Researcher position and Visiting Professor at Faculty of Pharmaceutical Sciences, UNESP. Since January 2022 she is Invited Assistant Professor in the Health Sciences Faculty of UBI and since March 2023 she holds a CEEC institutional position as Assistant Researcher at CICS-UBI. Her main research interests include the implementation of new biotechnological platforms to obtain and deliver biopharmaceuticals with potential applications in DNA vaccines or gene therapy approaches. From 2014, the Dr. Sousa's team was pioneer in the modification of monolithic chromatographic supports with amino acids and its derivative ligands to efficiently purify therapeutic nucleic acids (plasmids and minicircle DNA), by applying a single chromatographic step. Recently, she developed the first chromatographic analytical method for the assessment of minicircle DNA supercoiled isoform. She participates as PI, team member and collaborator in several financed research projects. She collaborates with several international institutions, namely The University of Texas at Austin, UNESP, IRCCS Regina Elena National Cancer Institute, CRBM, Montpellier University, Birla Institute of Technology & Science-Pilani, Hyderabad; international companies namely BIA Separations and SARTORIUS, and national institutions, namely UCIBIO-I4HB, iMed.ULisboa and CDRsp-IPL. During her scientific career, she has gained supervision experience of 39 trainees, 10 research grants, 46 Master students (6 ongoing) and 11 PhD students (10 ongoing, which the project "Mannosylated minicircle DNA nanovaccine against COVID-19" has been disseminated by TV show, radio web channels and magazines). She has also Jury and Referee experience of Graduation, Master and Doctoral thesis. Her scientific experience is summarized in the publication of 85 research articles (Hindex 19), showing her experience as independent researcher from the last 5 years in publications as corresponding author (26), work responsible/last author (23) and international collaborations (24). She also published 11 papers in conference proceedings, 7 book chapters (invited), 1 national patent (PT-106446; 110595), 4 publications of scientific work in Websites and participated in several national and international conferences (receiving 11 awards of best oral/poster presentations). She received several awards (ex. UBI/Santander young researcher in 2023) and is PI of DryVac project (2023.00136.RESTART) from FCT. She was Member of Scientific and Organizing Committees and Awards Committee of national/international conferences. She is a frequent referee of papers in international journals, member of the editorial boards of scientific journals, Guest and Associate Editor of 9 Special Issues,Editor of 3 Books from Springer Nature, 4 eBooks, Evaluator of National Innovation Agency and member of International network of Prevention to One Health. Her outreach activities include being invited speaker at conferences, Cancer day, Science Week, journals, radio, TV interviews
Identificação

Identificação pessoal

Nome completo
Ângela Maria Almeida de Sousa

Nomes de citação

  • Sousa, Ângela

Identificadores de autor

Ciência ID
FB1F-08DE-23FE
ORCID iD
0000-0001-9155-7581

Endereços de correio eletrónico

  • angela@fcsaude.ubi.pt (Profissional)
  • angsousa@ubi.pt (Profissional)

Domínios de atuação

  • Ciências Médicas e da Saúde - Biotecnologia Médica - Diagnóstico e Terapias de Base Genética

Idiomas

Idioma Conversação Leitura Escrita Compreensão Peer-review
Inglês Utilizador independente (B1) Utilizador independente (B1) Utilizador independente (B1) Utilizador independente (B1)
Francês Utilizador independente (B1) Utilizador independente (B1) Utilizador independente (B1) Utilizador independente (B1)
Formação
Grau Classificação
2023/03/20 - 2029/03/19
Em curso
CEEC Institucional position as Assistant Researcher at CICS-UBI (Outros)
Especialização em Health Biotechnology
Universidade da Beira Interior, Portugal
2018/09/01 - 2023/03/19
Concluído
Assistant Researcher (Outros)
Especialização em Health Biotechnology
Universidade da Beira Interior, Portugal
2015 - 2018
Concluído
Health Biotechnology (Pós-doutoramento)
Universidade da Beira Interior, Portugal
2012 - 2015
Concluído
Health Biotechnology (Pós-doutoramento)
Universidade da Beira Interior, Portugal
2011
Concluído
Bioquímica (Doutoramento)
Universidade da Beira Interior, Portugal
"Affinity chromatography in plasmid DNA purification for therapeutic applications" (TESE/DISSERTAÇÃO)
19 valores (Qualificação final de Excelente)
2007
Concluído
Bioquímica (Licenciatura)
Universidade da Beira Interior, Portugal
"Cromatografia de Interacção Hidrofóbica da proteose peptona 3" (TESE/DISSERTAÇÃO)
16
Percurso profissional

Ciência

Categoria Profissional
Instituição de acolhimento
Empregador
2023/03/20 - 2029/03/19 Investigador Auxiliar (carreira) (Investigação) Universidade da Beira Interior, Portugal
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2018/09/03 - 2023/03/19 Investigador Contratado (Investigação) Universidade da Beira Interior, Portugal
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2019/12/09 - 2019/12/13 Investigador visitante (Investigação) Universidade Estadual Paulista Júlio de Mesquita Filho - Câmpus de Araraquara, Brasil
Universidade Estadual Paulista Júlio de Mesquita Filho - Câmpus de Araraquara, Brasil
2015/09/01 - 2018/08/31 Pós-doutorado (Investigação) Universidade da Beira Interior, Portugal
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2012/01/01 - 2015/08/31 Pós-doutorado (Investigação) Universidade da Beira Interior, Portugal
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal

Docência no Ensino Superior

Categoria Profissional
Instituição de acolhimento
Empregador
2022/01/03 - Atual Professor Auxiliar Convidado (Docente Universitário) Universidade da Beira Interior, Portugal
2019/12/09 - 2019/12/13 Professor Visitante (Docente Universitário) Universidade Estadual Paulista Júlio de Mesquita Filho - Câmpus de Araraquara, Brasil
Universidade Estadual Paulista Júlio de Mesquita Filho - Câmpus de Araraquara, Brasil
2017/02/20 - 2018/07/31 Professor Auxiliar Convidado (Docente Universitário) Universidade da Beira Interior, Portugal
Universidade da Beira Interior, Portugal

Outros

Categoria Profissional
Instituição de acolhimento
Empregador
2015/07/22 - Atual Responsible researcher for the integrated internship on the program "Ciência Viva no Laboratório - Ocupação Científica de Jovens nas férias". Universidade da Beira Interior, Portugal
2015/07/01 - Atual Responsible researcher for the internship developed under Scientific International Exchanges (LORE) of MedUBI (Mobility Department of medical students), in the project "Preparing of a DNA vaccine for Universidade da Beira Interior, Portugal
2014/07/01 - Atual Member of the Editorial board from the international scientific journal Universidade da Beira Interior, Portugal
2012/05/01 - Atual Member of training commission of young researchers from Health Science Research Center Universidade da Beira Interior, Portugal
2012/01/01 - Atual Integrated member from the Health Science Research Center (CICS) Universidade da Beira Interior, Portugal
2012/01/01 - Atual Post-Doc in Biotechnology, with the project "Purification of HPV-16 E6/E7 DNA vaccine by monolithic chromatography using amino acids and derivatives as affinity ligands for pharmaceutical applications Universidade da Beira Interior, Portugal
2011/01/01 - Atual Responsible for the Chromatography lab in the Health Science Research Center Universidade da Beira Interior, Portugal
2009/09/21 - Atual Collaboration as University teaching in: Theoretical classes of Bioprocess Design, Biotechnology; Tutorial classes of Cellular Biology; Laboratorial classes of Pharmaceutical Biotechnology and Cellula Universidade da Beira Interior, Portugal
2015/01/14 - 2015/02/27 Practical and theoretical Workshop of animal experimentation (110 hours) - "II Course for personal researcher in biomedical sciences and users of experimental animals, class C" in Spain. Universidade da Beira Interior, Portugal
2013/12/02 - 2013/12/04 Chairman from the Oral section of the "Encontro Nacional de Cromatografia" Universidade da Beira Interior, Portugal
2013/07/01 - 2013/07/02 Chairman from the 2nd session of the "VIII Symposium CICS" Universidade da Beira Interior, Portugal
2012/07/02 - 2012/07/03 Chairman from the 1st session of the "VIII Symposium CICS" Universidade da Beira Interior, Portugal
2008/01/01 - 2011/12/31 PhD in Biochemistry, with the project "Affinity chromatography on plasmid DNA purification for therapeutic applications" (Refª SFRH/BD/41390/2007). Universidade da Beira Interior, Portugal
2011/03/26 - 2011/04/24 Scientific internship about chemistry immobilization of amino acid ligands in monolithic chromatographic supports produced by BIA Separations company in Slovenia. Universidade da Beira Interior, Portugal
2010/01/01 - 2011/01/01 Responsible for the Centrifuge lab in the Health Science Research Center Universidade da Beira Interior, Portugal
2009/05/01 - 2009/07/31 Scientific internship about plasmid DNA purification by multimodal chromatography, exploring innovative columns developed by GE Healthcare company in Lund University. Universidade da Beira Interior, Portugal
2006/09/15 - 2007/07/15 Curricular internship with the project: "Development of a purification strategy based on hydrophobic interaction chromatography of a milk protein, Proteose Peptone 3", Health Sciences Research Centre Universidade da Beira Interior, Portugal
Projetos

Bolsa

Designação Financiadores
2015 - 2018 Platform for the biosynthesis and purification of HPV-16 E6/E7mutant DNA vaccine for cervical cancer treatment or prevention
SFRH/BPD/102716/2014
Bolseiro de Pós-Doutoramento
Fundação para a Ciência e a Tecnologia
Concluído
2012 - 2015 Purification of HPV-16 E6/E7 DNA vaccine by monolithic chromatography using amino acids and derivatives as affinity ligands for pharmaceutical applications
SFRH/BPD/79106/2011
Bolseiro de Pós-Doutoramento
Fundação para a Ciência e a Tecnologia
Concluído
2008 - 2011 Affinity chromatography in plasmid DNA purification for therapeutic applications
SFRH/BD/41390/2007
Fundação para a Ciência e a Tecnologia

Projeto

Designação Financiadores
2023/09 - Atual ONCOSENSING
PROMOVE
Investigador responsável
Fundação 'la Caixa'
Em curso
2017 - Atual Gene Therapy for Mitochondrial DNA disease: Development of a mitochondrial-targeted vector
IF/01459/2015
Outra
Universidade da Beira Interior, Portugal
Fundação para a Ciência e a Tecnologia
Em curso
2023/01/01 - 2033/05/31 NanoINTer: Functionalized DNA Nanovaccine for Cervical Cancer Immunotherapy
INOVC+
Investigador responsável
Universidade da Beira Interior, Portugal
Em curso
2023/03/20 - 2029/03/19 MIRACLE: Dry powder mannosylated MIniciRcle DNA nanovaccine against CervicaL cancEr
CEEC Institucional project
Investigador responsável
Universidade da Beira Interior, Portugal
Fundação para a Ciência e a Tecnologia
Em curso
2023/09/01 - 2025/02/28 DryVac: Dry powder mannosylated minicircle DNA nanoVaccine against Cervical Cancer
2023.00136.RESTART
Investigador responsável
Universidade da Beira Interior, Portugal
Fundação para a Ciência e a Tecnologia
Em curso
2023/03/01 - 2024/02/29 MIND: Multifunctional Innovative NanoDevice for lung cancer therapy
UBI/SANTANDER
Investigador responsável
Banco Santander Portugal
Em curso
2020/11/01 - 2022/10/31 Development of a metabolomic model of therapeutic individualization and prognosis for medically assisted procreation
15
Investigador
Em curso
2020/11/01 - 2022/08/31 Screening of potential synthetic and natural inhibitors of HPV E6 and E7 oncoproteins - Therapeutic approach against cervical cancer.
20
Investigador responsável
Concluído
2018/07/01 - 2021/06/30 Suportes modificados com Liquidos Iónicos para a Purificação de pré-miRNAs envolvidos na doença de Alzheimer
PTDC/BII-BBF/29496/2017
Universidade de Aveiro, Portugal

Universidade da Beira Interior, Portugal
Fundação para a Ciência e a Tecnologia
Em curso
2019/01/01 - 2019/12/31 Health Sciences Research Centre
UID/Multi/00709/2019
Universidade da Beira Interior, Portugal

Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
2014 - 2016 Ligandos do G-quadruplex para terapia do cancro
EXPL/QEQ-MED/1068/2013
Outra
Universidade da Beira Interior, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
2013 - 2015 Bioseparation and targeted delivery of pre-miR-29 for Alzheimer disease application
EXPL/BBBBIO/1056/2012
Outra
Universidade da Beira Interior, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
2011/03/01 - 2014/08/31 Biosynthesis of highly pure plasmid DNA for cancer therapy
PTDC/EBB-BIO/114320/2009
Investigador
Universidade da Beira Interior, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
2011 - 2014 Isolation and purification of DNA plasmid for cancer therapy
PTDC/EBBBIO/114320/2009
Fundação para a Ciência e a Tecnologia
Concluído
2011/01/01 - 2013/12/31 Strategic Project - UI 709 - 2011-2012
PEst-C/SAU/UI0709/2011
Universidade da Beira Interior, Portugal

Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
2007 - 2013 Technologies for purification and controlled release of biopharmaceuticals to be applied in age-related diseases
CENTRO-07-ST24-FEDER-002014
Investigador
Concluído
2007 - 2010 Synthesis, optimization and intensification of bioseparation processes for DNA vaccine manufacturing
PTDC/EQU-EQU/65492/2006
Outra
Universidade da Beira Interior, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
Produções

Publicações

Artigo em conferência
  1. Diana Pereira; Susana Ferreira; Nuno Alves; Ângela Sousa; Joana F. A. Valente. "Chitosan–Silver Nanoconjugates to Achieve Enhanced Antimicrobial Effect and Fight Cervical Cancer". 2022.
    10.3390/materproc2022008129
  2. Daniela Carvalho; Diana Pereira; Nuno Alves; Andreas Pickl; Sara Cardoso; Joana F. A. Valente; Ângela Sousa. "New Combined Approach to Simplify the Minicircular DNA Lysate and Directly Apply in Ion Exchange Membrane Chromatography". 2022.
    10.3390/materproc2022008041
  3. Diana Pereira; Susana Ferreira; Nuno Alves; Ângela Sousa; Joana F. A. Valente. "Dopamine-Coated Silver Nanoparticles: Therapeutic Agents to Fight Bacteria and Fungi". 2022.
    10.3390/materproc2022008028
  4. Nunes, Renato; Sousa, Ângela; Simaite, Aiva; Aido, Ahmed; Buzgo, Matej. "Sub-100 nm chitosan-triphosphate-DNA nanoparticles for delivery of DNA vaccines". 2020.
    10.3390/iecp2020-08653
  5. Sousa, Ângela. "Screening of L-histidine based ligands to purify the supercoiled plasmid DNA isoform". Trabalho apresentado em IEEE 4th Portuguese Meeting on Bioengineering, Porto, 2015.
  6. Sousa, Ângela. "Amino acid derivatized monoliths for purification of a DNA vaccine against influenza". Trabalho apresentado em IEEE 4th Portuguese Meeting on Bioengineering, Porto, 2015.
  7. Sousa, Ângela. "Plasmid DNA purification by integrating membrane technology with arginine affinity chromatography". Trabalho apresentado em 16th European Congress on Biotechnology, 2014.
  8. Sousa, Ângela. "Rapid quantification of supercoiled plasmid deoxyribonucleic acid using a monolithic ion exchanger". 2012.
  9. Sousa, Ângela. "Performance of a non-grafted monolithic support for purification of supercoiled plasmid DNA". Trabalho apresentado em International Symposium on Protiens, Peptides and Polynucleotides (ISPPP), 2012.
  10. Sousa, Ângela. "“Development of Chitosan Nanoparticles Loaded with Purified Supercoiled Plasmid DNA Biopharmaceuticals for Cancer Gene Therapy". 2011.
  11. Sousa, Ângela. "New strategies for plasmid DNA purification using aqueous two phase systems and multimodal chromatography". 2010.
Artigo em revista
  1. Eusébio, Dalinda; Paul, Milan; Biswas, Swati; Cui, Zhengrong; Costa, Diana; Sousa., Ângela. "Mannosylated polyethylenimine-cholesterol-based nanoparticles for targeted delivery of minicircle DNA vaccine against COVID-19 to antigen-presenting cells". International Journal of Pharmaceutics (2024): 123959. http://dx.doi.org/10.1016/j.ijpharm.2024.123959.
    10.1016/j.ijpharm.2024.123959
  2. Diana Pereira; Susana Ferreira; Gloria Belén Ramírez-Rodríguez; Nuno Alves; Ângela Sousa; Joana F. A. Valente. "Silver and Antimicrobial Polymer Nanocomplexes to Enhance Biocidal Effects". International Journal of Molecular Sciences (2024): https://doi.org/10.3390/ijms25021256.
    10.3390/ijms25021256
  3. Gomes, D.; Batista-Silva, J.P.; Sousa, A.; Passarinha, L.A.. "Progress and opportunities in Gellan gum-based materials: A review of preparation, characterization and emerging applications". Carbohydrate Polymers 311 (2023): 120782. http://dx.doi.org/10.1016/j.carbpol.2023.120782.
    10.1016/j.carbpol.2023.120782
  4. Gomes, D.; Correia, M.A.S.; Romão, M.J.; Passarinha, L.A.; Sousa, A.. Autor correspondente: Sousa, A.. "Integrated approaches for the separation and purification of recombinant HPV16 E6 protein from Escherichia coli crude extracts". Separation and Purification Technology 315 (2023): 123647. http://dx.doi.org/10.1016/j.seppur.2023.123647.
    10.1016/j.seppur.2023.123647
  5. Sousa, Ângela; Soares, Christiane P.; Chin, Chung Man; Trisciuoglio, Daniela; Valdes-Mora, Fatima. Autor correspondente: Sousa, Ângela. "Editorial: Epigenetic therapy against cancer: toward new molecular targets and technologies". Frontiers in Cell and Developmental Biology 11 (2023): http://dx.doi.org/10.3389/fcell.2023.1218986.
    10.3389/fcell.2023.1218986
  6. Miguel Ferreira; Diana Gomes; Miguel Neto; Luís A. Passarinha; Diana Costa; Ângela Sousa. Autor correspondente: Ângela Sousa. "Development and Characterization of Quercetin-Loaded Delivery Systems for Increasing Its Bioavailability in Cervical Cancer Cells". Pharmaceutics (2023): https://doi.org/10.3390/pharmaceutics15030936.
    10.3390/pharmaceutics15030936
  7. Rúben Faria; Prisca Boisguérin; Ângela Sousa; Diana Costa. "Delivery Systems for Mitochondrial Gene Therapy: A Review". Pharmaceutics (2023): https://doi.org/10.3390/pharmaceutics15020572.
    10.3390/pharmaceutics15020572
  8. João Batista-Silva; Diana Gomes; Jorge Barroca-Ferreira; Eugénia Gallardo; Ângela Sousa; Luís A. Passarinha. "Specific Six-Transmembrane Epithelial Antigen of the Prostate 1 Capture with Gellan Gum Microspheres: Design, Optimization and Integration". International Journal of Molecular Sciences (2023): https://doi.org/10.3390/ijms24031949.
    10.3390/ijms24031949
  9. Ana Teresa Brinca; Ofélia Anjos; Maria Manuel Casteleiro Alves; Ângela Sousa; António Hélio Oliani; Luiza Breitenfeld; Luís A. Passarinha; Ana Cristina Ramalhinho; Eugenia Gallardo. "Volatilomics as an Emerging Strategy to Determine Potential Biomarkers of Female Infertility: A Pilot Study". Biomedicines (2022): https://doi.org/10.3390/biomedicines10112852.
    10.3390/biomedicines10112852
  10. Ana Raquel Neves; Tânia Albuquerque; Rúben Faria; Ana M. Gonçalves; Cecília Santos; Eric Vivès; Prisca Boisguérin; et al. "Development of WRAP5 Peptide Complexes for Targeted Drug/Gene Co-Delivery toward Glioblastoma Therapy". Pharmaceutics (2022): https://doi.org/10.3390/pharmaceutics14102213.
    10.3390/pharmaceutics14102213
  11. Cathy Ventura; Ângelo Luís; Christiane P. Soares; Aldo Venuti; Francesca Paolini; Luísa Pereira; Ângela Sousa. Autor correspondente: Ângela Sousa. "The Effectiveness of Therapeutic Vaccines for the Treatment of Cervical Intraepithelial Neoplasia 3: A Systematic Review and Meta-Analysis". Vaccines (2022): https://doi.org/10.3390/vaccines10091560.
    10.3390/vaccines10091560
  12. Ana M. Gonçalves; Ângela Sousa; Augusto Q. Pedro; Maria J. Romão; João A. Queiroz; Eugénia Gallardo; Luís A. Passarinha. "Advances in Membrane-Bound Catechol-O-Methyltransferase Stability Achieved Using a New Ionic Liquid-Based Storage Formulation". International Journal of Molecular Sciences 23 13 (2022): 7264-7264. https://doi.org/10.3390/ijms23137264.
    10.3390/ijms23137264
  13. Diana Gomes; Shivani Yaduvanshi; Samuel Silvestre; Ana Paula Duarte; Adriana O. Santos; Christiane P. Soares; Veerendra Kumar; Luís Passarinha; Ângela Sousa. Autor correspondente: Ângela Sousa. "Taxifolin and Lucidin as Potential E6 Protein Inhibitors: p53 Function Re-Establishment and Apoptosis Induction in Cervical Cancer Cells". Cancers 14 12 (2022): 2834-2834. https://doi.org/10.3390/cancers14122834.
    10.3390/cancers14122834
  14. Ana Teresa Brinca; Ana Cristina Ramalhinho; Ângela Sousa; António Hélio Oliani; Luiza Breitenfeld; Luís A. Passarinha; Eugenia Gallardo. "Follicular Fluid: A Powerful Tool for the Understanding and Diagnosis of Polycystic Ovary Syndrome". Biomedicines 10 6 (2022): 1254-1254. https://doi.org/10.3390/biomedicines10061254.
    10.3390/biomedicines10061254
  15. Miguel Ferreira; Diana Costa; Ângela Sousa. Autor correspondente: Ângela Sousa. "Flavonoids-Based Delivery Systems towards Cancer Therapies". Bioengineering 9 5 (2022): 197-197. https://doi.org/10.3390/bioengineering9050197.
    10.3390/bioengineering9050197
  16. Cathy Ventura; Dalinda Eusébio; Ana M. Gonçalves; Jorge Barroca-Ferreira; Diana Costa; Zhengrong Cui; Luís A. Passarinha; Ângela Sousa. Autor correspondente: Ângela Sousa. "Maximization of the Minicircle DNA Vaccine Production Expressing SARS-CoV-2 RBD". Biomedicines 10 5 (2022): 990-990. https://doi.org/10.3390/biomedicines10050990.
    10.3390/biomedicines10050990
  17. Ângela Sousa; Christiane P. Soares; Aldo Venuti. Autor correspondente: Ângela Sousa. "Vaccines against Infectious Diseases and Cancer". Vaccines (2022): https://doi.org/10.3390/vaccines10050648.
    10.3390/vaccines10050648
  18. Nunes, Renato; Serra, Ana Sofia; Simaite, Aiva; Sousa, Ângela. Autor correspondente: Sousa, Ângela. "Modulation of Chitosan-TPP Nanoparticle Properties for Plasmid DNA Vaccines Delivery". Polymers 14 7 (2022): 1443. http://dx.doi.org/10.3390/polym14071443.
    10.3390/polym14071443
  19. Rúben Faria; Milan Paul; Swati Biswas; Eric Vivès; Prisca Boisguérin; Ângela Sousa; Diana Costa. "Peptides vs. Polymers: Searching for the Most Efficient Delivery System for Mitochondrial Gene Therapy". Pharmaceutics (2022): https://doi.org/10.3390/pharmaceutics14040757.
    10.3390/pharmaceutics14040757
  20. Ângela Sousa; Ana Margarida Almeida; Joana Valente; João Queiroz; Fani Sousa. "Hands-On Laboratory Class for Biopharmaceutical pDNA Quality Control". Journal of Chemical Education (2022): https://doi.org/10.1021/acs.jchemed.1c00695.
    10.1021/acs.jchemed.1c00695
  21. Diana Pereira; Tiago Soares Carreira; Nuno Alves; Ângela Sousa; Joana F. A. Valente. "Metallic Structures: Effective Agents to Fight Pathogenic Microorganisms". International Journal of Molecular Sciences 23 3 (2022): 1165-1165. https://doi.org/10.3390/ijms23031165.
    10.3390/ijms23031165
  22. Diana Gomes; Ângela Sousa; Luís A. Passarinha. "Tyrosinase Immobilization in Nickel-Cross-Linked Gellan Microspheres and Conversion of l-DOPA to Dopachrome". Journal of Chemical Education 98 11 (2021): 3560-3566. https://doi.org/10.1021/acs.jchemed.1c00176.
    10.1021/acs.jchemed.1c00176
  23. Gomes, D.; Costa, D.; Queiroz, J.A.; Passarinha, L.A.; Sousa, A.. "A new insight in gellan microspheres application to capture a plasmid DNA vaccine from an Escherichia coli lysate". Separation and Purification Technology 274 (2021): 119013. http://dx.doi.org/10.1016/j.seppur.2021.119013.
    10.1016/j.seppur.2021.119013
  24. Sousa, Ângela; Barud, Hernane Da Silva; Xu, Zhi Ping; Soares, Christiane P.. "Editorial: Nanotechnology for Precision Cancer Therapy: Advances in Gene Therapy, Immunotherapy, and 3D Bioprinting". Frontiers in Nanotechnology 3 (2021): http://dx.doi.org/10.3389/fnano.2021.776898.
    10.3389/fnano.2021.776898
  25. Carlos Rodolfo; Dalinda Eusébio; Cathy Ventura; Renato Nunes; Helena F. Florindo; Diana Costa; Ângela Sousa. "Design of Experiments to Achieve an Efficient Chitosan-Based DNA Vaccine Delivery System". Pharmaceutics 13 9 (2021): 1369-1369. https://doi.org/10.3390/pharmaceutics13091369.
    10.3390/pharmaceutics13091369
  26. Ana Raquel Neves; Tânia Albuquerque; Rúben Faria; Milan Paul; Swati Biswas; Ângela Sousa; Diana Costa. "Development of Tailor-Made Dendrimer Ternary Complexes for Drug/Gene Co-Delivery in Cancer". Pharmaceutics 13 8 (2021): 1256-1256. https://doi.org/10.3390/pharmaceutics13081256.
    10.3390/pharmaceutics13081256
  27. Diana Gomes; Samuel Silvestre; Ana Paula Duarte; Aldo Venuti; Christiane P. Soares; Luís Passarinha; Ângela Sousa. "In Silico Approaches: A Way to Unveil Novel Therapeutic Drugs for Cervical Cancer Management". Pharmaceuticals (2021): https://doi.org/10.3390/ph14080741.
    10.3390/ph14080741
  28. Faria, Rúben; Vivés, Eric; Boisguerin, Prisca; Sousa, Angela; Costa, Diana. "Development of Peptide-Based Nanoparticles for Mitochondrial Plasmid DNA Delivery". Polymers 13 11 (2021): 1836. http://dx.doi.org/10.3390/polym13111836.
    10.3390/polym13111836
  29. Serra, Ana Sofia; Eusébio, Dalinda; Neves, Ana Raquel; Albuquerque, Tânia; Bhatt, Himanshu; Biswas, Swati; Costa, Diana; Sousa, Ângela. "Synthesis and Characterization of Mannosylated Formulations to Deliver a Minicircle DNA Vaccine". Pharmaceutics 13 5 (2021): 673. http://dx.doi.org/10.3390/pharmaceutics13050673.
    10.3390/pharmaceutics13050673
  30. Sousa, Ângela; Christiane Pienna Soares; Jean Leandro Dos Santos. "Epigenetic Therapy with Histone Deacetylase Inhibitors: Implications for Cancer Treatment". Frontiers in Cell and Developmental Biology 662761 (2021):
    Acesso aberto • Aceite para publicação
  31. Sousa, Ângela. "Effect of Plasmid DNA Size on Chitosan or Polyethyleneimine Polyplexes Formulation". Polymers 13 (2021): 793. https://doi.org/10.3390/polym13050793.
    Acesso aberto • Publicado
  32. Eusébio, Dalinda; Almeida, Ana Margarida; Alves, Joel Marques; Maia, Cláudio Jorge; Queiroz, João António; Sousa, Fani; Sousa, Ângela. "The Performance of Minicircle DNA Versus Parental Plasmid in p53 Gene Delivery Into HPV-18-Infected Cervical Cancer Cells". Nucleic Acid Therapeutics 31 1 (2021): 82-91. http://dx.doi.org/10.1089/nat.2020.0904.
    10.1089/nat.2020.0904
  33. Lourenço de Freitas, Natália; Deberaldini, Maria Gabriela; Gomes, Diana; Pavan, Aline Renata; Sousa, Ângela; Dos Santos, Jean Leandro; Soares, Christiane P.. "Histone Deacetylase Inhibitors as Therapeutic Interventions on Cervical Cancer Induced by Human Papillomavirus". Frontiers in Cell and Developmental Biology 8 (2021): http://dx.doi.org/10.3389/fcell.2020.592868.
    10.3389/fcell.2020.592868
  34. Sousa, Ângela. "Epigenetic Therapy with Histone Deacetylase Inhibitors: Implications for Cancer Treatment". Frontiers Cell and Developmental Biology (2021):
    Acesso aberto • Aceite para publicação
  35. Eusébio, Dalinda; Neves, Ana Raquel; Costa, Diana; Biswas, Swati; Alves, Gilberto; Cui, Zhengrong; Sousa, Ângela. "Methods to improve the immunogenicity of plasmid DNA vaccines". Drug Discovery Today (2021):
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  36. Ana Margarida Almeida; João António Queiroz; Fani Sousa; Ângela Sousa. "Minicircle DNA: The Future for DNA-Based Vectors?". Trends in Biotechnology (2020): https://doi.org/10.1016/j.tibtech.2020.04.008.
    10.1016/j.tibtech.2020.04.008
  37. Sousa, Ângela. "Enhancement of a biotechnological platform for the purification and delivery of a human papillomavirus supercoiled plasmid DNA vaccine". New Biotechnology (2020):
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  38. Sousa, Ângela. "Polymer-peptide ternary systems as a tool to improve the properties of plasmid DNA vectors in gene delivery". Journal of Molecular Liquids (2020): http://dx.doi.org/10.1016/j.molliq.2020.113157.
    10.1016/j.molliq.2020.113157
  39. J.F.A. Valente; A. Sousa; G.A. Azevedo; J.A. Queiroz; F. Sousa. "Purification of supercoiled p53-encoding plasmid using an arginine-modified macroporous support". Journal of Chromatography A (2020): 460890-460890. https://doi.org/10.1016/j.chroma.2020.460890.
    10.1016/j.chroma.2020.460890
  40. Almeida, A.M.; Cernigoj, U.; Queiroz, J.A.; Sousa, F.; Sousa, A.. "Quality assessment of supercoiled minicircle DNA by cadaverine-modified analytical chromatographic monolith". Journal of Pharmaceutical and Biomedical Analysis 180 (2020): http://www.scopus.com/inward/record.url?eid=2-s2.0-85076629062&partnerID=MN8TOARS.
    10.1016/j.jpba.2019.113037
  41. Sousa, Â.; Faria, R.; Albuquerque, T.; Bhatt, H.; Biswas, S.; Queiroz, J.A.; Costa, D.. "Design of experiments to select triphenylphosphonium-polyplexes with suitable physicochemical properties for mitochondrial gene therapy". Journal of Molecular Liquids 302 (2020): http://www.scopus.com/inward/record.url?eid=2-s2.0-85078098670&partnerID=MN8TOARS.
    10.1016/j.molliq.2020.112488
  42. Sousa, Ângela. "Composite Central Face Design-An Approach to Achieve Efficient Alginate Microcarriers.". Polymers (2019): https://doi.org/10.3390/polym11121949.
    10.3390/polym11121949
  43. Ângela Sousa; Ana M. Almeida; Rúben Faria; Karidia Konate; Prisca Boisguerin; João A. Queiroz; Diana Costa. "Optimization of peptide-plasmid DNA vectors formulation for gene delivery in cancer therapy exploring design of experiments". Colloids and Surfaces B: Biointerfaces (2019): 110417-110417. https://doi.org/10.1016/j.colsurfb.2019.110417.
    10.1016/j.colsurfb.2019.110417
  44. A.R. Neves; A. Sousa; R. Faria; T. Albuquerque; J.A. Queiroz; D. Costa. "Cancer gene therapy mediated by RALA/plasmid DNA vectors: nitrogen to phosphate groups ratio (N/P) as a tool for tunable transfection efficiency and apoptosis". Colloids and Surfaces B: Biointerfaces (2019): https://doi.org/10.1016/j.colsurfb.2019.110610.
    10.1016/j.colsurfb.2019.110610
  45. A.M. Almeida; D. Eusébio; J.A. Queiroz; F. Sousa; A. Sousa. "The use of size-exclusion chromatography in the isolation of supercoiled minicircle DNA from Escherichia coli lysate". Journal of Chromatography A (2019): 460444-460444. https://doi.org/10.1016/j.chroma.2019.460444.
    10.1016/j.chroma.2019.460444
  46. Faria, R.; Sousa, Â.; Neves, A.R.; Queiroz, J.A.; Costa, D.. "Methotrexate-plasmid DNA polyplexes for cancer therapy: Characterization, cancer cell targeting ability and tuned in vitro transfection". Journal of Molecular Liquids 292 (2019): http://www.scopus.com/inward/record.url?eid=2-s2.0-85069657438&partnerID=MN8TOARS.
    10.1016/j.molliq.2019.111391
  47. Coelho, J.; Eusébio, D.; Gomes, D.; Frias, F.; Passarinha, L.A.; Sousa, Â.. "Biosynthesis and isolation of gellan polysaccharide to formulate microspheres for protein capture". Carbohydrate Polymers 220 (2019): 236-246. http://www.scopus.com/inward/record.url?eid=2-s2.0-85066399568&partnerID=MN8TOARS.
    10.1016/j.carbpol.2019.05.011
  48. Almeida, A.M.; Queiroz, J.A.; Sousa, F.; Sousa, A.. "Minicircle DNA purification: Performance of chromatographic monoliths bearing lysine and cadaverine ligands". Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 1118-1119 (2019): 7-16. http://www.scopus.com/inward/record.url?eid=2-s2.0-85064324749&partnerID=MN8TOARS.
    10.1016/j.jchromb.2019.04.024
  49. Almeida, A.M.; Queiroz, J.A.; Sousa, F.; Sousa, Â.. "Cervical cancer and HPV infection: ongoing therapeutic research to counteract the action of E6 and E7 oncoproteins". Drug Discovery Today (2019): http://www.scopus.com/inward/record.url?eid=2-s2.0-85070489597&partnerID=MN8TOARS.
    10.1016/j.drudis.2019.07.011
  50. Valente, J.F.A.; Sousa, A.; Queiroz, J.A.; Sousa, F.. "DoE to improve supercoiled p53-pDNA purification by O-phospho-L-tyrosine chromatography". Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 1105 (2019): 184-192. http://www.scopus.com/inward/record.url?eid=2-s2.0-85059140598&partnerID=MN8TOARS.
    10.1016/j.jchromb.2018.12.002
  51. Azevedo, G.M.; Valente, J.F.A.; Sousa, A.; Pedro, A.Q.; Pereira, P.; Sousa, F.; Queiroz, J.A.. "Effect of chromatographic conditions on supercoiled plasmid DNA stability and bioactivity". Applied Sciences (Switzerland) 9 23 (2019): http://www.scopus.com/inward/record.url?eid=2-s2.0-85076894415&partnerID=MN8TOARS.
    10.3390/app9235170
  52. Sousa, Ângela. "Arginine homopeptides for plasmid DNA purification using monolithic supports". Journal of Chromatography B (2018): https://doi.org/10.1016/j.jchromb.2018.04.031.
    10.1016/j.jchromb.2018.04.031
  53. Ana M. Almeida; Joana Tomás; Patrícia Pereira; João A. Queiroz; Fani Sousa; Ângela Sousa. "HPV-16 targeted DNA vaccine expression: The role of purification". Biotechnology Progress (2018): https://doi.org/10.1002/btpr.2603.
    10.1002/btpr.2603
  54. Costa, D.; Valente, A.J.M.; Queiroz, J.A.; Sousa, Â.. "Finding the ideal polyethylenimine-plasmid DNA system for co-delivery of payloads in cancer therapy". Colloids and Surfaces B: Biointerfaces 170 (2018): 627-636. http://www.scopus.com/inward/record.url?eid=2-s2.0-85049322981&partnerID=MN8TOARS.
    10.1016/j.colsurfb.2018.06.063
  55. Valente, J.F.A.; Sousa, A.; Gaspar, V.M.; Queiroz, J.A.; Sousa, F.. "The biological performance of purified supercoiled p53 plasmid DNA in different cancer cell lines". Process Biochemistry 75 (2018): 240-249. http://www.scopus.com/inward/record.url?eid=2-s2.0-85054460579&partnerID=MN8TOARS.
    10.1016/j.procbio.2018.09.014
  56. Lúcia F. A. Amorim; Rita Gaspar; Patrícia Pereira; Urh Cernigoj; Fani Sousa; João António Queiroz; Ângela Sousa. "Chromatographic HPV-16 E6/E7 plasmid vaccine purification employing L-histidine and 1-benzyl-L-histidine affinity ligands". ELECTROPHORESIS (2017): https://doi.org/10.1002/elps.201700147.
    10.1002/elps.201700147
  57. Bicho, D.; Caramelo-Nunes, C.; Sousa, A.; Sousa, F.; Queiroz, J.A.; Tomaz, C.T.. "Purification of influenza deoxyribonucleic acid-based vaccine using agmatine monolith". Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 1012-1013 (2016): 153-161. http://www.scopus.com/inward/record.url?eid=2-s2.0-84955605658&partnerID=MN8TOARS.
    10.1016/j.jchromb.2015.12.036
  58. Diamantino, T.; Pereira, P.; Queiroz, J.A.; Sousa, Â.; Sousa, F.. "Minicircle DNA purification using a CIM® DEAE-1 monolithic support". Journal of Separation Science 39 18 (2016): 3544-3549. http://www.scopus.com/inward/record.url?eid=2-s2.0-84988447206&partnerID=MN8TOARS.
    10.1002/jssc.201600375
  59. Almeida, A.M.; Queiroz, J.A.; Sousa, F.; Sousa, A.. "Optimization of supercoiled HPV-16 E6/E7 plasmid DNA purification with arginine monolith using design of experiments". Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 978-979 (2015): 145-150. http://www.scopus.com/inward/record.url?eid=2-s2.0-84919800600&partnerID=MN8TOARS.
    10.1016/j.jchromb.2014.12.004
  60. Bicho, D.; Santos, B.F.; Caramelo-Nunes, C.; Sousa, A.; Sousa, F.; Queiroz, J.A.; Tomaz, C.T.. "Application of ethylenediamine monolith to purify a hemagglutinin influenza deoxyribonucleic acid-based vaccine". Separation and Purification Technology 154 (2015): 320-327. http://www.scopus.com/inward/record.url?eid=2-s2.0-84943535397&partnerID=MN8TOARS.
    10.1016/j.seppur.2015.09.046
  61. Amorim, L.F.A.; Sousa, F.; Queiroz, J.A.; Cruz, C.; Sousa, A.. "Screening of l -histidine-based ligands to modify monolithic supports and selectively purify the supercoiled plasmid DNA isoform". Journal of Molecular Recognition 28 6 (2015): 349-358. http://www.scopus.com/inward/record.url?eid=2-s2.0-84928111894&partnerID=MN8TOARS.
    10.1002/jmr.2449
  62. Bicho, D.; Sousa, A.; Sousa, F.; Queiroz, J.; Tomaz, C.. "Effect of chromatographic conditions and plasmid DNA size on the dynamic binding capacity of a monolithic support". Journal of Separation Science 37 17 (2014): 2284-2292. http://www.scopus.com/inward/record.url?eid=2-s2.0-84906848855&partnerID=MN8TOARS.
    10.1002/jssc.201400127
  63. Mota, E.; Sousa, F.; Sousa, Â.; Queiroz, J.A.; Cruz, C.. "Molecular recognition of oligonucleotides and plasmid DNA by L-methionine". Journal of Molecular Recognition 27 10 (2014): 588-596. http://www.scopus.com/inward/record.url?eid=2-s2.0-84906888435&partnerID=MN8TOARS.
    10.1002/jmr.2382
  64. Sousa, T.; Pereira, P.; Sousa, F.; Queiroz, J.A.. "Binding mechanisms for histamine and agmatine ligands in plasmid deoxyribonucleic acid purifications". Journal of Chromatography A 1366 (2014): 110-119. http://www.scopus.com/inward/record.url?eid=2-s2.0-84907969355&partnerID=MN8TOARS.
    10.1016/j.chroma.2014.09.031
  65. Pereira, P.; Sousa, T.; Queiroz, J.A.; Figueiras, A.; Sousa, F.. "Pharmaceutical-grade pre-miR-29 purification using an agmatine monolithic support". Journal of Chromatography A 1368 (2014): 173-182. http://www.scopus.com/inward/record.url?eid=2-s2.0-84908209237&partnerID=MN8TOARS.
    10.1016/j.chroma.2014.09.080
  66. Nunes, C.; Sousa, A.; Nunes, J.C.; Morão, A.M.; Sousa, F.; Queiroz, J.A.. "Supercoiled plasmid DNA purification by integrating membrane technology with a monolithic chromatography". Journal of Separation Science 37 11 (2014): 1229-1236. http://www.scopus.com/inward/record.url?eid=2-s2.0-84902117888&partnerID=MN8TOARS.
    10.1002/jssc.201301160
  67. Pereira, P.; Sousa, T.; Queiroz, J.; Figueiras, A.; Sousa, F.. "New approach for purification of pre-miR-29 using lysine-affinity chromatography". Journal of Chromatography A 1331 (2014): 129-132. http://www.scopus.com/inward/record.url?eid=2-s2.0-84896715699&partnerID=MN8TOARS.
    10.1016/j.chroma.2014.01.033
  68. Cruz, C.; Sousa, Â.; Mota, É.; Sousa, F.; Queiroz, J.A.. "Quantitative analysis of histamine- and agmatine-DNA interactions using surface plasmon resonance". International Journal of Biological Macromolecules 70 (2014): 131-137. http://www.scopus.com/inward/record.url?eid=2-s2.0-84904357281&partnerID=MN8TOARS.
    10.1016/j.ijbiomac.2014.06.044
  69. Gonçalves, A.I.C.; Rocha, L.A.; Dias, J.M.L.; Passarinha, L.A.; Sousa, A.. "Optimization of a chromatographic stationary phase based on gellan gum using central composite design". Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 957 (2014): 46-52. http://www.scopus.com/inward/record.url?eid=2-s2.0-84896500707&partnerID=MN8TOARS.
    10.1016/j.jchromb.2014.02.050
  70. Pereira, P.; Sousa, Â.; Queiroz, J.; Correia, I.; Figueiras, A.; Sousa, F.. "Purification of pre-miR-29 by arginine-affinity chromatography". Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 951-952 1 (2014): 16-23. http://www.scopus.com/inward/record.url?eid=2-s2.0-84893705000&partnerID=MN8TOARS.
    10.1016/j.jchromb.2014.01.020
  71. Valente, J.F.A.; Sousa, A.; Queiroz, J.A.; Sousa, F.. "Selective purification of supercoiled p53-encoding pDNA with l-methionine-agarose matrix". Analytical Biochemistry 459 (2014): 61-69. http://www.scopus.com/inward/record.url?eid=2-s2.0-84902657783&partnerID=MN8TOARS.
    10.1016/j.ab.2014.05.011
  72. Afonso, A.; Pereira, P.; Queiroz, J.A.; Sousa, T.; Sousa, F.. "Purification of pre-miR-29 by a new O-phospho-l-tyrosine affinity chromatographic strategy optimized using design of experiments". Journal of Chromatography A 1343 (2014): 119-127. http://www.scopus.com/inward/record.url?eid=2-s2.0-84899989051&partnerID=MN8TOARS.
    10.1016/j.chroma.2014.03.071
  73. Sousa, A.; Almeida, A.M.; Cernigoj, U.; Sousa, F.; Queiroz, J.A.. "Histamine monolith versatility to purify supercoiled plasmid deoxyribonucleic acid from Escherichia coli lysate". Journal of Chromatography A 1355 (2014): 125-133. http://www.scopus.com/inward/record.url?eid=2-s2.0-84904286739&partnerID=MN8TOARS.
    10.1016/j.chroma.2014.06.003
  74. Soares, A.; Queiroz, J.A.; Sousa, F.; Sousa, A.. "Purification of human papillomavirus 16 E6/E7 plasmid deoxyribonucleic acid-based vaccine using an arginine modified monolithic support". Journal of Chromatography A 1320 (2013): 72-79. http://www.scopus.com/inward/record.url?eid=2-s2.0-84887611735&partnerID=MN8TOARS.
    10.1016/j.chroma.2013.10.053
  75. Cruz, C.; Sousa, A.; Sousa, F.; Queiroz, J.A.. "Affinity analysis between immobilized l-arginine and plasmid isoforms provided by surface plasmon resonance". Analytical Methods 5 7 (2013): 1682-1686. http://www.scopus.com/inward/record.url?eid=2-s2.0-84879509327&partnerID=MN8TOARS.
    10.1039/c3ay40213d
  76. Mota, É.; Sousa, Â.; Cernigoj, U.; Queiroz, J.A.; Tomaz, C.T.; Sousa, F.. "Rapid quantification of supercoiled plasmid deoxyribonucleic acid using a monolithic ion exchanger". Journal of Chromatography A 1291 (2013): 114-121. http://www.scopus.com/inward/record.url?eid=2-s2.0-84876791056&partnerID=MN8TOARS.
    10.1016/j.chroma.2013.03.070
  77. Sousa, Â.; Sousa, F.; Queiroz, J.A.. "Advances in chromatographic supports for pharmaceutical-grade plasmid DNA purification". Journal of Separation Science 35 22 (2012): 3046-3058. http://www.scopus.com/inward/record.url?eid=2-s2.0-84870040472&partnerID=MN8TOARS.
    10.1002/jssc.201200307
  78. Cruz, C.; Sousa, A.; Sousa, F.; Queiroz, J.A.. "Study of the specific interaction between l-methionine chromatography support and nucleotides". Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 909 (2012): 1-5. http://www.scopus.com/inward/record.url?eid=2-s2.0-84868249738&partnerID=MN8TOARS.
    10.1016/j.jchromb.2012.09.037
  79. Sousa, A.; Tomaz, C.T.; Sousa, F.; Queiroz, J.A.. "Successful application of monolithic innovative technology using a carbonyldiimidazole disk to purify supercoiled plasmid DNA suitable for pharmaceutical applications". Journal of Chromatography A 1218 46 (2011): 8333-8343. http://www.scopus.com/inward/record.url?eid=2-s2.0-80054865157&partnerID=MN8TOARS.
    10.1016/j.chroma.2011.09.033
  80. Gaspar, V.M.; Correia, I.J.; Sousa, A.; Silva, F.; Paquete, C.M.; Queiroz, J.A.; Sousa, F.. "Nanoparticle mediated delivery of pure P53 supercoiled plasmid DNA for gene therapy". Journal of Controlled Release 156 2 (2011): 212-222. http://www.scopus.com/inward/record.url?eid=2-s2.0-81255211172&partnerID=MN8TOARS.
    10.1016/j.jconrel.2011.08.007
  81. Sousa, A.; Bicho, D.; Tomaz, C.T.; Sousa, F.; Queiroz, J.A.. "Performance of a non-grafted monolithic support for purification of supercoiled plasmid DNA". Journal of Chromatography A 1218 13 (2011): 1701-1706. http://www.scopus.com/inward/record.url?eid=2-s2.0-79952363162&partnerID=MN8TOARS.
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  82. Sousa, A.; Sousa, F.; Queiroz, J.A.. "Impact of lysine-affinity chromatography on supercoiled plasmid DNA purification". Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 879 30 (2011): 3507-3515. http://www.scopus.com/inward/record.url?eid=2-s2.0-80555149385&partnerID=MN8TOARS.
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  83. Sousa, A.; Sousa, F.; Queiroz, J.A.. "Differential interactions of plasmid DNA, RNA and genomic DNA with amino acid-based affinity matrices". Journal of Separation Science 33 17-18 (2010): 2610-2618. http://www.scopus.com/inward/record.url?eid=2-s2.0-77956918746&partnerID=MN8TOARS.
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  84. Sousa, A.; Sousa, F.; Prazeres, D.M.F.; Queiroz, J.A.. "Histidine affinity chromatography of homo-oligonucleotides. Role of multiple interactions on retention". Biomedical Chromatography 23 7 (2009): 745-753. http://www.scopus.com/inward/record.url?eid=2-s2.0-69749128313&partnerID=MN8TOARS.
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  85. Sousa, Â.; Sousa, F.; Queiroz, J.A.. "Selectivity of arginine chromatography in promoting different interactions using synthetic oligonucleotides as model". Journal of Separation Science 32 10 (2009): 1665-1672. http://www.scopus.com/inward/record.url?eid=2-s2.0-67650001336&partnerID=MN8TOARS.
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  86. Sousa, A.; Sousa, F.; Queiroz, J.A.. "Biorecognition of supercoiled plasmid DNA isoform in lysine-affinity chromatography". Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 877 27 (2009): 3257-3260. http://www.scopus.com/inward/record.url?eid=2-s2.0-69749124060&partnerID=MN8TOARS.
    10.1016/j.jchromb.2009.08.021
  87. Sousa, A.; Passarinha, L.A.; Rodrigues, L.R.; Teixeira, J.A.; Mendonça, A.; Queiroz, J.A.. "Separation of different forms of proteose peptone 3 by hydrophobic interaction chromatography with a dual salt system". Biomedical Chromatography 22 5 (2008): 447-449. http://www.scopus.com/inward/record.url?eid=2-s2.0-44249099939&partnerID=MN8TOARS.
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Capítulo de livro
  1. Ana R. Neves; Albuquerque T; Eusébio, Dalinda; Sousa, Ângela; Costa, Diana. "Challenges and future perspectives of natural biopolymers in drug delivery". In Natural Biopolymers for Drug Delivery. Elsevier, 2024.
    Aceite para publicação
  2. Gomes, Diana; Passarinha, Luís A.; Sousa, Ângela. Autor correspondente: Sousa, Ângela. "E6 Tagged Protein Production, Extraction, and Purification from Escherichia coli Lysate". In Advanced Methods in Structural Biology, 21-33. Springer US, 2023.
    10.1007/978-1-0716-3147-8_2
  3. Neves, Ana R.; Biswas, Swati; Sousa, Ângela; Costa, Diana. "Nanoconjugates and nanoconjugate formulations for improving drug delivery and therapeutic efficacy". In Advanced Nanoformulations, 397-430. Elsevier, 2023.
    10.1016/b978-0-323-85785-7.00020-6
  4. Neves, Ana R.; Faria, Rúben; Albuquerque, Tânia; Quintela, Telma; Sousa, Ângela; Costa, Diana. "Future perspectives of biological macromolecules in biomedicine". In Biological Macromolecules, 607-632. Elsevier, 2022.
    10.1016/b978-0-323-85759-8.00026-9
  5. Faria, Rúben; Albuquerque, Tânia; Neves, Ana Raquel; Sousa, Ângela; Costa, Diana Rita Barata. "Nanotechnology to Correct Mitochondrial Disorders in Cancer Diseases". In Cancer Nanotechnology, 179-203. Springer International Publishing, 2022.
    10.1007/978-3-031-17831-3_6
  6. Eusébio, Dalinda; Albuquerque, Tânia; Neves, Ana R.; Faria, Rúben; Ventura, Cathy; Ferreira, Miguel; Sousa, Ângela. "Drug delivery for bone tissue engineering". In ADVANCED AND MODERN APPROACHES FOR DRUGDELIVERY. Elsevier, 2022.
    No prelo
  7. Sousa, Ângela. "Minicircle DNA Vaccine Purification and E7 Antigen Expression Assessment". In DNA Vaccines. Humana Press, 2020.
    Aceite para publicação
  8. Sousa, Ângela. "Conception of Plasmid DNA and Polyethylenimine delivery Systems with Potential Application in DNA Vaccines Field". In DNA Vaccines. Humana Press, 2020.
    Aceite para publicação
  9. Sousa, Â.; Queiroz, J.A.; Sousa, F.. "p53-encoding pDNA purification by affinity chromatography for cancer therapy". 2015.
    10.1007/978-1-4939-2727-2_7
Edição de livro
  1. Sousa, Ângela; Luís A. Passarinha. Advanced Methods in Structural Biology. Springer Nature. 2023.
    Submetido
  2. Â.M. Almeida de Sousa. Cancer Nanotechnology. Springer International Publishing. 2023.
    10.1007/978-3-031-17831-3
  3. Sousa, Ângela. Epigenetic Therapy with Histone Deacetylase Inhibitors: Implications for Cancer Treatment. Frontiers Media SA. 2021.
    10.3389/978-2-88966-835-9
  4. Â. Sousa. DNA Vaccines. Springer US. 2021.
    10.1007/978-1-0716-0872-2
  5. Sousa, Ângela. Autor correspondente: Sousa, Ângela. Nanotechnology for Precision Cancer Therapy: Advances in gene therapy, immunotherapy, and 3D bioprinting. Frontiers Media SA. 2021.
    10.3389/978-2-88971-821-4
Edição de número de revista
  1. Luís Passarinha. "Recent Advances in Bioseparation Technologies for the Capture of Emerging Biomacromolecules". International Journal of Molecular Sciences (2023):
    Editor convidado
  2. Sousa, Ângela. "Epigenetic Therapy Against Cancer: Toward New Molecular Targets and Technologies". Frontiers in Cell and Developmental Biology (2022):
    Em revisão
  3. Sousa, Ângela. "Nanotechnology for Precision Cancer Therapy: Advances in gene therapy, immunotherapy, and 3D bioprinting - Volume II". Frontiers in Nanotechnology (2022):
    Em revisão
  4. Sousa, Ângela. "Polymeric nanoparticles as drug delivery systems". Frontiers in Biomaterials Science (2022):
  5. Sousa, Ângela. "Vaccines against Infectious Diseases and Cancer". Vaccines, MDPI (2021):
    Editor convidado
  6. Sousa, Ângela. "Biopolymers: Recent Progress and New Perspectives". Polymers, MDPI (2021):
    Editor convidado
  7. Sousa, Ângela. "Nanotechnology for Precision Cancer Therapy: Advances in gene therapy, immunotherapy, and 3D bioprinting". Frontiers in Nanotechnology (2021):
    Editor associado
  8. Sousa, Ângela. "Biopolymers: Recent Progress and New Perspectives II". Polymers (2021): https://www.mdpi.com/journal/polymers/special_issues/biopoly_recent_progre_new_perspect_II.
    Editor convidado
  9. Sousa, Ângela. "Epigenetic Therapy with Histone Deacetylase Inhibitors: Implications for Cancer Treatment". Frontiers in Cell and Developmental Biology (2020):
    Editor associado
Poster em conferência
  1. Eusébio, Dalinda; Ventura, Cathy; Gonçalves, Ana M.; Barroca-Ferreira, Jorge; Costa, Diana; Cui, Zhengrong; Passarinha, Luís A.; Sousa, Ângela. "Optimization of the minicircle DNA vaccine expressing SARS-CoV-2 RBD production using orbital shaker experiments and a mini-bioreactor platform". Trabalho apresentado em IV International Congress on Health Sciences Research Towards Innovation and Entrepreneurship - Challenges from Research to Clinics, 2023.
  2. Pereira, Diana; Ferreira, Susana; ALVES, NUNO; Sousa, Ângela; Valente, Joana F. A.. "Silver-based nanoparticles as powerful pathogen-killing agents". Trabalho apresentado em Biosystems in Toxicology and Pharmacology ¿ Current challenges, 2022.
  3. Eusébio, Dalinda; Paul, Milan; Biswas, Swati; Cui, Zhengrong; Costa, Diana; Sousa, Ângela. "Multifunctional nanoparticles for targeted delivery of minicircle DNA vaccine against COVID-19 to antigen-presenting cells". Trabalho apresentado em XVII International CICS-UBI Symposium, 2022.
  4. Pereira, Diana; Ferreira, Susana; Nuno Alves; Sousa, Ângela; Joana Valente. "Nanoconjugates of metal and polymers with improved antimicrobial properties". 2022.
  5. Pereira, Diana; Ferreira, Susana; Nuno Alves; Sousa, Ângela; Joana Valente. "Nanocomplexes of Silver and Polymer to Fight Pathogens". Trabalho apresentado em Encontro Ciência 22, 2022.
Website
  1. Sousa, Ângela. Autor correspondente: Sousa, Ângela. Taxifolin and Lucidin as Potential E6 Protein Inhibitors: p53 Function Re-Establishment and Apoptosis Induction in Cervical Cancer Cells. 2022. https://www.aspic.pt/pt-pt/taxifolina-e-lucidina-como-potenciais-inibidores-da-prote%C3%ADna-e6-restabelecimento-da-fun%C3%A7%C3%A3o-de-p53-e#.Y4YJiHbP3D4.
  2. Sousa, Ângela. The performance of minicircle DNA versus parental plasmid in p53 gene delivery into HPV-18-infected cervical cancer cells. 2021. http://www.aspic.pt/pt-pt/compara%C3%A7%C3%A3o-entre-os-vetores-de-dna-minicircular-e-plasm%C3%ADdeo-parental-na-entrega-do-gene-p53-c%C3%A9lulas#.YEKMVY77TD5.
  3. Sousa, Ângela. In Silico Approaches: A Way to Unveil Novel Therapeutic Drugs for Cervical Cancer Management. 2021. http://www.aspic.pt/pt-pt/abordagens-silico-uma-forma-de-descobrir-novos-f%C3%A1rmacos-para-o-tratamento-do-cancro-do-colo-do-%C3%BAtero?fbclid=IwAR0MLdxgs1AeGmZJmvu5eCQBtzf95WQVvMI6IHj53QOP3Ro6_g2xm5Xx--M#.YROuRIhKjD5.
  4. Sousa, Ângela. Cervical cancer and HPV infection: ongoing therapeutic research to counteract the action of E6 and E7 oncoproteins. 2019. http://www.aspic.pt/pt-pt/node/6864#.YEKKjo77TD5.

Propriedade Intelectual

Patente
  1. Sousa, Ângela. 2020. "Formulation of gelan microspheres and application in the capture of proteins from complex cell lysates". Portugal.
  2. Rocha, Luis António; Martins, Rita; Silva, Filomena; Sousa, Ângela; Passarinha, Luis António. 2012. "Chromatographic matrix based in gelan gum polymer". Portugal.
    Concedida/Emitida
  3. 2012. "Matriz cromatográfica baseada no polímero polissacárido gelana".

Outros

Técnica de investigação
  1. 12º Encontro Nacional de Cromatografia. 06/12/2022 - 08/12/2022 Aveiro Poster submission: "Determinação de potenciais biomarcadores de infertilidade feminina: estudo piloto".. Brinca, Ana Teresa; Anjos, Ofélia; Maria Manuel Casteleiro Alves; Sousa, Ângela; António Hélio Oliani; Luiza Breitenfeld; Passarinha, Luís; Ana Cristina Ramalhinho; Gallardo, Eugenia.
Atividades

Apresentação oral de trabalho

Título da apresentação Nome do evento
Anfitrião (Local do evento)
2023/12 New generation of DNA vaccines against cancers induced by HPV infection Congress of Microbiology and Biotechnology 2023
(Covilhã, Portugal)
2023/11 Volatilomics as an Emerging Strategy to Determine Potential Biomarkers of Female Infertility: A Pilot Study 31st World Congress on Controversies in Obstetrics, Gynecology and Infertility (COGI)
(Austrália)
2023/06 Recombinant biosynthesis and purification of high-risk HPV E7 protein for anti-HPV drug development Affinity 2023
Universidade Nova de Lisboa (Lisboa, Portugal)
2023/06 Exploring synthetic compounds as HPV E6 protein inhibitors for cervical cancer management Affinity 2023
Universidade Nova de Lisboa (Lisboa, Portugal)
2023/06 Targeting HPV E6 protein: computational and experimental analysis of synthetic compounds 3rd Meeting of Young Biophysicists
Instituto de Investigação e Inovação em Saúde (Porto, Portugal)
2023/04 Innovative approach for lung cancer therapy Lung cancer – From innovation in diagnosis to advances in the investigation of new therapies
Universidade da Beira Interior (Covilhã, Portugal)
2023/02 An in silico approach to find potential HPV E6 oncoprotein inhibitors for personalized cervical cancer therapy IV International Congress in Health Science Research – Challenges from Research to Clinics
Universidade da Beira Interior (Covilhã, Portugal)
2023/02 Unveiling HPV E6 protein potential inhibitors against cervical cancer: From E6 biosynthesis to in silico and biophysical studies International Congress in Health Science Research – Challenges from Research to Clinics
Universidade da Beira Interior (Covilha, Portugal)
2023/02 Enhanced Effect of Silver Nanoparticles Coated with Antimicrobial Polymers Against Pathogenic Microorganisms IV International Congress in Health Science Research – Challenges from Research to Clinics
Universidade da Beira Interior (Covilha, Portugal)
2022/11 Rally of Biopharmaceuticals - Seminar Week of Science and Technology
2022/09 Are vaccines safe? - Research Marathon UNIGHT in the “UNITA edition” of the European Researchers' Night
2022/02 Cancer research: Different perspectives from CICS-UBI, Covilhã round table
2022 Impacts on the prevention of HPV infections and its vaccination during the Covid-19 pandemic in Portugal Integrative communication action for health prevention: Strategies to reduce the impacts of the pandemic
UNESP, Araraquara, Brazil
2022 Recombinant E6 protein production and isolation: a target for cervical cancer internacional workshop Path/mVACCIL/IL2BioPro
CICECO, Universidade de Aveiro
2022 From biosynthesis to purification of recombinant E6 protein: a therapeutic target for cervical cancer Biopartitioning & Purification conference (BPP)
CICECO, Universidade de Aveiro
2021/11 Cancer Vaccines development The Pharmacist and Oncology: Current and future
VII UBIPharma - Universidade da Beira Interior (Covilhã, Portugal)
2021/11 Rally of Biopharmaceuticals - Seminar week of Science and Technology
(Portugal)
2021/04 Mannosylated DNA nanovaccine for intranasal administration against COVID-19 COVID-19 from theory to practice: Projects developed during the pandemic
Nucleus of Students of IEEE- Instituto Superior Técnico and EMBS (Lisboa, Portugal)
2021/02 COVID-19 Vaccination: Nucleic Acid Vaccines COVID-19 Awareness Week
Nucleus of Students of Pharmaceutical Sciences at UBI (UBIPharma) (Covilhã, Portugal)
2021 Innovation and Access to Personalized Therapeutics VII UBIPharma Annual Scientific Congress “The Pharmacist and Oncology: Current and future”
Universidade da Beira Interior
2020/12 Sub-100 nm chitosan-triphosphate-DNA nanoparticles for delivery of DNA vaccines The 1st International Electronic Conference on Pharmaceutics
MDPI
2019/11/12 DNA vaccine against HPV infection: How to obtain it?
School of Pharmaceutical Sciences, Sao Paulo State University (UNESP) (Araraquara, Brasil)
2019 Gellan microsphere formulation for direct capture of Soluble catechol-O-methyltransferase from a Komagataella pastoris lysate 13th International Meeting of the Portuguese Carbohydrate Group – GLUPOR 13
(Porto, Portugal)
2019 Gellan exopolysaccharide biosynthesis and recovery to formulate microspheres optimized by Design of experiments 13th International Meeting of the Portuguese Carbohydrate Group – GLUPOR 13
(Portugal)
2019 Soluble catechol-O-methyltransferase capture from a Pichia pastoris X33 lysate through a new batch method with gellan microspheres 38th International Symposium on the Separation of Proteins, Peptides and Polynucleotides (ISPPP)
(Alemanha)
2019 Workshop Biofármacos no Laboratório V Congresso Científico Anual UBIPharma – Antibióticos: até quando?
Health Sciences faculty of University of Beira Interior (Covilhã, Portugal)
2018 Pri-mir-375 expressing mcDNA vector: assessing the role in HPV oncoproteins silencing 12º International Symposium “European Society of Biochemical Engineering Sciences” (ESBES)
(Portugal)
2018 Sample displacement chromatography – old principle for modern applications 8th Monolith Summer School (MSS)
(Eslovénia)
2018 Workshop Laboratorial em Biotecnologia Farmacêutica IV Congresso Científico Anual UBIPharma – Inovar e Reinventar a Prática Farmacêutica
Health Sciences faculty of University of Beira Interior (Covilhã, Portugal)
2017 Development and optimization of new monolithic adsorbents for plasmid DNA purification by affinity chromatography Anything but conventional chromatography (Abc2)
(Portugal)
2017 Affinity chromatography for Minicircle DNA purification: lysine- and cadaverine-monoliths performance II International Congress in Health Sciences Research: Towards Innovation and Entrepreneurship - Trends in Biotechnology and Biomedical Applications
(Portugal)
2017 Workshop prático de Biotecnologia: implementação de uma plataforma biotecnológica para a obtenção de um biofármaco III Congresso Científico Anual UBIPharma - Pharma goes Digital
Health Sciences faculty of University of Beira Interior (Covilhã, Portugal)
2016 Superporous affinity chromatography for the supercoiled p53 encoding plasmid isolation ISPPP2016 - International Symposium on the Separation of Proteins, Peptides and Polynucleotides
(Austrália)
2016 p53 supercoiled encoding plasmid in different cancer cell lines: Transfection and Expression effects Bio.Iberoamérica 2016
(Espanha)
2016 Versatility of arginine monolithic support in the purification of therapeutic DNA vectors 9º Encontro Nacional de Cromatografia
(Portugal)
2015 DNA vaccine development for HPV treatment: biotechnological production, purification and in vitro application Micro Biotech’14 - Portuguese Congress of Microbiology and Biotechnology
(Portugal)
2015 Supercoiled HPV16 E6/E7 plasmid DNA-based vaccine purified by affinity chromatography for in vitro transfection studies VIII Jornadas sobre Tecnologia e Saúde
(Portugal)
2015 Purification of a DNA vaccine against Human Papillomavirus infection using L-histidine and 1-benzyl-L-histidine monoliths VIII Jornadas sobre Tecnologia e Saúde
(Portugal)
2015 Screening of L-histidine based ligands to purify the supercoiled plasmid DNA isoform 4th Portuguese BioEngineering Meeting
(Portugal)
2015 Amino acid derivatized monoliths for purification of a DNA vaccine against influenza 4th Portuguese BioEngineering Meeting
(Portugal)
2014 Modification of monolithic columns by immobilization of amino acids and derivatives to purify the plasmid DNA vaccine against HPV-16 6th Monolith Summer School & Symposium 2014
(Eslovénia)
2013 Purification of Human Papillomavirus16 E6/E7 plasmid DNA-based vaccine using an arginine modified monolithic support Micro Biotech’13 - Portuguese Congress of Microbiology and Biotechnology
(Portugal)
2013 Molecular recognition of polynucleotides and plasmid DNA by L-methionine support 8º Encontro Nacional de Cromatografia
(Portugal)
2013 Pre-miR-29 purification by amino acids-affinity chromatography 8º Encontro Nacional de Cromatografia
(Portugal)
2013 Study of the interactions between immobilized L-methionine and oligonucleotides by chromatography, NMR and SPR analysis 19th International Symposium on Separation Sciences – New achievements in Chromatography
(Croácia)
2013 Gellan gum: A new approach in biomolecules purification 1st International Conference on Natural Fibers
(Portugal)
2012 Development of pDNA purification process using single monolithic chromatographic column SBCN2012 - Bioseparation & Characterization
(França)
2012 A new monolithic strategy for pharmaceutical-grade plasmid DNA purification 7º Encontro Nacional de Cromatografia
(Portugal)
2012 Purification of supercoiled plasmid DNA by affinity chromatography for therapeutic applications in Biotechnology
Polytechnic Institute of Guard (Guarda, Portugal)
2012 Purification of plasmids for application in DNA vaccines and gene therapy – Application to biotechnology
Faculty of Sciences of University of Beira Interior (Covilhã, Portugal)
2012 Purification technologies of a DNA vaccine to prevent and treat the HPV infection Biotechnology Forum
INESPO (Inovation Network Spain-Portugal) (Covilhã, Portugal)
2010 Performance of a new methacrylate monolith for supercoiled plasmid DNA dynamic adsorption SBCN - 12th International meeting and workshop of the Society for Biochromatography and Nanoseparations
(França)
2009 New lysine-affinity chromatography for biorecognition of supercoiled plasmid DNA isoform Affinity 2009
(Islândia)
2009 Arginine-affinity chromatography: selectivity for oligonucleotides biorecognition BPP – International Conference on Biopartitioning and Purification
(Reino Unido)

Orientação

Título / Tema
Papel desempenhado
Curso (Tipo)
Instituição / Organização
2024 - 2028 A new life in Citrus peels: from an agroindustrial waste to a promising antifungal biotherapeutic
Coorientador
Biomedicina (Doutoramento)
Universidade da Beira Interior, Portugal

Università degli Studi di Torino Sistema Bibliotecario d'Ateneo, Itália
2024/01/01 - 2027/12/31 In silico and in vitro Bioactivity Evaluation of Novel STEAP1 Antagonists for Prostate Cancer (FCT ref: 2023.04376.BD)
Coorientador
Biomedicina (Doutoramento)
Universidade da Beira Interior, Portugal
2022/11/01 - 2026/10 Targeted Delivery of small Molecules for Cancer therapy
Orientador
Biomedicina (Doutoramento)
Universidade da Beira Interior, Portugal
2022/11/01 - 2026/10 Multifunctional lipid nanoparticles for lung cancer therapy
Coorientador
Biomedicina (Doutoramento)
Universidade da Beira Interior, Portugal
2022/10/01 - 2026/09/30 TRiNa4CC - Transition metal nanoparticles and Gene therapy: Combined approach for cervical cancer (FCT ref: 2022.12947.BD)
Orientador
Bioquímica (Doutoramento)
Universidade da Beira Interior, Portugal
2021/09/01 - 2025/08/31 miniGEN4kill - Biotechnological platform based on gene therapy with minicircle DNA and antiviral nanoparticles to treat and fight cervical cancer (FCT ref: 2021.09331.BD)
Orientador
Biomedicina (Doutoramento)
Universidade da Beira Interior, Portugal
2021/01/01 - 2024/12/31 Mannosylated minicircle DNA nanovaccine against COVID-19 (mixed Grant, FCT ref: 2020.10201.BD)
Orientador
Biomedicina (Doutoramento)
Universidade da Beira Interior, Portugal
2021/01/01 - 2024/12/31 Biointeration studies of recombinant HPV-16 oncoproteins to discover therapeutic inhibitors (mixed Grant, FCT ref: 2020.06792.BD)
Coorientador
Bioquímica (Doutoramento)
Universidade da Beira Interior, Portugal
2023/09 - 2024/07 Development of a functionalized minicircular DNA nanovaccine with potential immunotherapeutic action
Orientador
Ciências Biomédicas (Mestrado)
Universidade da Beira Interior, Portugal
2023/09 - 2024/07 Glioblastoma Therapy: Study of the therapeutic capacity of a co-release nano-system
Coorientador
Ciências Biomédicas (Mestrado)
Universidade da Beira Interior, Portugal
2023/09 - 2024/07 Novel delivery systems based on functionalized gellan nanoparticles for Parkinson's disease therapeutics
Coorientador
Biotecnologia (Mestrado)
Universidade da Beira Interior, Portugal
2023/09 - 2024/07 3D printing of anti-HPV drugs for the treatment of cervical cancer
Coorientador
Bioquímica (Mestrado)
Universidade da Beira Interior, Portugal
2020/04 - 2024/04 Therapeutic strategies for the treatment of Human Papillomavirus (HPV) infections: Comparison of the effect of the HPV pDNA-E7 vaccine on different nanosystems (International doctorate, cotutela between UNESP/Brazil and UBI/Portugal)
Orientador
Ciências, Citologia e Biologia Celular / Bioquímica (Doutoramento)
Universidade Estadual Paulista Júlio de Mesquita Filho - Câmpus de Araraquara, Brasil

Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2023/12 - 2024/03 Fulbright fellowship: Synthesis and characterization of new chromatographic resins, aimed at purifying proteins that are difficult to isolate.
Orientador
University of Virginia Department of Engineering Systems and Environment, Estados Unidos
2022/09 - 2023/10 Development of peptide-based vectors for mitochondrial targeting
Coorientador
Biotecnologia (Mestrado)
Universidade da Beira Interior, Portugal
2023/05 - 2023/09 Unveiling potent STEAP1 antagonist ligands by integration of molecular docking and in vitro studies (doctoral incentive scholarship, financed from Santander)
Orientador
Biomedicina (Doutoramento)
Universidade da Beira Interior, Portugal
2022/09/19 - 2023 Potential inhibitors of the HPV E6 oncoprotein for personalized cervical cancer therapy
Orientador
Ciências Biomédicas (Mestrado)
Universidade da Beira Interior, Portugal
2022/09/19 - 2023 Development of ceramic nanoconjugates with mcDNA for the treatment of cervical cancer
Orientador
Bioquímica (Mestrado)
Universidade da Beira Interior, Portugal
2022/09/19 - 2023 Plasmid DNA-loaded calcium phosphate functional nanocomplexes: innovative approach against cervical cancer
Orientador
Biotecnologia (Mestrado)
Universidade da Beira Interior, Portugal
2022/09/19 - 2023 Formulation and characterization of taxifolin delivery systems as and anti-Human Papillomavirus drug
Orientador
Ciências Biomédicas (Mestrado)
Universidade da Beira Interior, Portugal
2019 - 2023 Mitochondrial Gene Therapy: Development of a mitochondrial targeted peptide/plasmid DNA vector (mixed Grant, FCT ref: SFRH/BD/148393/2019)
Coorientador
Biomedicina (Doutoramento)
Universidade da Beira Interior, Portugal
2022/10/01 - 2022/12/20 external fellowship from the Short-Term Research Internship at the University of Texas at Austin to convert the DNA nanovaccine into dry powder for intranasal administration.
Orientador
Universidade da Beira Interior, Portugal

The University of Texas at Austin Biodiversity Center, Estados Unidos
2021/09/20 - 2022/11/23 Application of Gellan Microspheres for the Isolation of the Six-Transmembrane Epithelial Antigen of the Prostate 1 (STEAP1) Protein
Coorientador
Biotecnologia (Mestrado)
Universidade da Beira Interior, Portugal
2021/09/20 - 2022/11/22 Delivery of anti-HPV drugs to cervical cancer cells
Orientador
Ciências Biomédicas (Mestrado)
Universidade da Beira Interior, Portugal
2021/09/19 - 2022/11/22 Obtaining a minicircular DNA vaccine against COVID-19
Orientador
Ciências Biomédicas (Mestrado)
Universidade da Beira Interior, Portugal
2022/09/01 - 2022/09/30 Multidisciplinary Experience in Research to fight Cancer and Infection (MERCI) project funded by special support “Summer with Science” - Chitosan-based systems to deliver an anti-HPV compound to cervical cancer cells
Orientador
Universidade da Beira Interior, Portugal
2022/09/01 - 2022/09/30 Multidisciplinary Experience in Research to fight Cancer and Infection (MERCI) project funded by special support “Summer with Science” - In silico screening of potential HPV E7 oncoprotein inhibitors
Orientador
Universidade da Beira Interior, Portugal
2021/09/20 - 2022 Production of membranes by electrospinning for filtration or clarification of a minicircular DNA vector.
Orientador
Biotecnologia (Mestrado)
Universidade da Beira Interior, Portugal
2021/09/01 - 2021/09/30 Summer school from UCIBIO project funded by special support of “Summer with Science” - In silico studies of flavonoid-derived small molecules and their potential therapeutic action on cervical cancer cells
Coorientador
REQUIMTE Unidade de Ciências Biomoleculares Aplicadas, Portugal
2021/09/01 - 2021/09/30 Multidisciplinary Experience in Research to fight Cancer and Infection (MERCI) project funded by special support “Summer with Science” - Application of gellan microspheres to directly capture/isolate STEAP1 from Komagataella pastoris lysates
Orientador
Universidade da Beira Interior, Portugal
2021/09/01 - 2021/09/30 Multidisciplinary Experience in Research to fight Cancer and Infection (MERCI) project funded by special support “Summer with Science” - Optimization of production conditions for a minicircular DNA vaccine against COVID-19
Orientador
Universidade da Beira Interior, Portugal
2020 - 2021 Assessment of the impact of containment measures on the transmission of SARS-CoV-2 on the incidence of respiratory infections in the 2020-21 season
Coorientador
Bioquímica (Mestrado)
Universidade da Beira Interior, Portugal
2020 - 2021 Evaluation of "low haemoglobin density" and "microcytic anemia factor" as early parameters of ferropenia
Coorientador
Bioquímica (Mestrado)
Universidade da Beira Interior, Portugal
2020 - 2021 Development of a functionalized minicircular DNA nanovaccine with potential immunotherapeutic action
Orientador
Bioquímica (Mestrado)
Universidade da Beira Interior, Portugal
2020 - 2021 Chitosan-triphosphate-DNA nanoparticles for delivery of DNA vaccines
Orientador
Bioengenharia (Mestrado)
Universidade da Beira Interior, Portugal
2019 - 2021 Targeted delivery to dendritic cells: Functionalization of a minicircular DNA vector with ligands
Orientador
Biotecnologia (Mestrado)
Universidade da Beira Interior, Portugal
2020/02/01 - 2020/12/31 Obtainment and targeted delivery of a minicircle DNA vaccine against Human Papillomavirus-associated cervical cancer (incentive scholarship to the doctorate, financed from Santander)
Orientador
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2019 - 2020 Epidemiologic study of incidence of SARS-CoV-2 in Beira Interior
Coorientador
Bioquímica (Mestrado)
Universidade da Beira Interior, Portugal
2019 - 2020 Design and development of innovative plasmid DNA transport systems
Coorientador
Bioengenharia (Mestrado)
Universidade da Beira Interior, Portugal
2018/09/15 - 2019/10/15 Proteomic study of the therapeutic effect of a DNA vector on cervical cancer cells
Orientador
Biotecnologia (Mestrado)
Universidade da Beira Interior, Portugal
2018/09/15 - 2019/10/12 In vitro evaluation of the synergistic effect of a multigenic vector of minicircular DNA in cervical cancer cells
Orientador
Ciências Biomédicas (Mestrado)
Universidade da Beira Interior, Portugal
2018/09/15 - 2019/10/11 Application of gelan spheres to capture or purify a plasmid DNA vaccine
Orientador
Biotecnologia (Mestrado)
Universidade da Beira Interior, Portugal
2018/09/15 - 2019/07/13 New plasmid DNA delivery systems: Design, development and in vitro evaluation
Coorientador
Biotecnologia (Mestrado)
Universidade da Beira Interior, Portugal
2015/04/01 - 2019/07 Biosynthesis and purification of minicircle DNA encoding pri-miR-375 for treatment of Human Papillomavirus infection (FCT ref: SFRH/BD/102284/2014)
Orientador
Bioquímica (Doutoramento)
Universidade da Beira Interior, Portugal
2017/09 - 2018/11 Capture strategies of human catechol-Omethyltransferase from Komagataella pastoris lysates by gellan microspheres
Coorientador
Química Medicinal (Mestrado)
Universidade da Beira Interior, Portugal
2017/09/15 - 2018/10/15 Multigenic minicircular DNA with potential therapeutic action in the cervical cancer
Orientador
Ciências Biomédicas (Mestrado)
Universidade da Beira Interior, Portugal
2017/09/16 - 2018/10/12 Cervical cancer therapy: Evaluation of therapeutic effect of the combined action with a drug and a plasmid DNA
Coorientador
Ciências Biomédicas (Mestrado)
Universidade da Beira Interior, Portugal
2016/09 - 2017/10 Obtaining of a plasmid DNA vector and target delivery to the tumor cells
Orientador
Biotecnologia (Mestrado)
Universidade da Beira Interior, Portugal
2016/09 - 2017/10 Minicircle DNA vectors with potential therapeutic action in cervical cancer
Orientador
Bioquímica (Mestrado)
Universidade da Beira Interior, Portugal
2016/09 - 2017/07 Biosynthesis, Isolation and Magnetization of gellan spheres for biorecognition of therapeutic His-tag proteins
Coorientador
Ciências Biomédicas (Mestrado)
Universidade da Beira Interior, Portugal
2015/09 - 2016/10 Obtaining a DNA vaccine for prevention or treatment of cervical cancer
Orientador
Ciências Biomédicas (Mestrado)
Universidade da Beira Interior, Portugal
2015/09 - 2016/10 Biorecognition of DNA minicircle by amino acids derivatives
Coorientador
Bioquímica (Mestrado)
Universidade da Beira Interior, Portugal
2015/09 - 2016/07 Development of purification strategies of SCOMT and MBCOMT proteins by affinity chromatography
Coorientador
Bioquímica (Mestrado)
Universidade da Beira Interior, Portugal
2015/09 - 2016/07 Purification of a plasmid DNA vaccine for prevention or treatment of cervical cancer
Orientador
Ciências Biomédicas (Mestrado)
Universidade da Beira Interior, Portugal
2014/10 - 2015/07 Development of new methodologies for DNA minicircle purification using monolithic supports
Coorientador
Bioquímica (Mestrado)
Universidade da Beira Interior, Portugal
2014/09 - 2015/07 Purification of a plasmid DNA vaccine against HPV
Orientador
Biotecnologia (Mestrado)
Universidade da Beira Interior, Portugal
2014/09 - 2015/07 Obtaining a plasmid DNA vaccine against HPV
Orientador
Bioquímica (Mestrado)
Universidade da Beira Interior, Portugal
2014/09 - 2015/07 Influence of purification and stabilization technology in the biological activity of miRNA
Coorientador
Biotecnologia (Mestrado)
Universidade da Beira Interior, Portugal
2014/07 - 2015/07 Application of a gellan gum support for recombinant proteins or nucleic acids partition
Orientador
Bioquímica (Mestrado)
Universidade da Beira Interior, Portugal
2013/07 - 2014/07 Purification of plasmid DNA HPV-16 E6/E7 vaccine and in vitro and in vivo immunogenicity evaluation
Orientador
Ciências Biomédicas (Mestrado)
Universidade da Beira Interior, Portugal
2013/07 - 2014/07 Purification of supercoiled HPV-16 E6/E7 plasmid using a modified monolithic support
Orientador
Ciências Biomédicas (Mestrado)
Universidade da Beira Interior, Portugal
2013/07 - 2014/07 Development of a new microcarrier culture based in matrix of natural polymer
Coorientador
Ciências Biomédicas
Universidade da Beira Interior, Portugal
2012/07 - 2013/07 Preparation of the HPV-16 E6/E7 DNA vaccine: purification with modified monoliths and in vitro evaluation
Orientador
Ciências Biomédicas (Mestrado)
Universidade da Beira Interior, Portugal
2012/07 - 2013/07 Development and optimization of a microRNA purification technology by affinity chromatography, using the concept of QbD
Coorientador
Biotecnologia (Mestrado)
Universidade da Beira Interior, Portugal
2012/07 - 2013/07 Development of new chromatographic support based on gellan gum for pharmaceutical biomolecules isolation
Coorientador
Bioquímica (Mestrado)
Universidade da Beira Interior, Portugal

Organização de evento

Nome do evento
Tipo de evento (Tipo de participação)
Instituição / Organização
2021 - Atual Workshop: "Rally dos Biofármacos" held during the week of Science and Technology (2021)
Oficina (workshop) (Coorganizador)
2015/07 - Atual Ciência Viva no Laboratório - Ocupação Científica de Jovens nas férias (2015/07)
Oficina (workshop) (Coorganizador)
Universidade da Beira Interior, Portugal
2019 - 2019 III International Congress in Health Sciences Research: Towards Innovation and Entrepreneurship - Trends in Aging and Cancer (2019/11/14 - 2019/11/16)
Congresso (Membro da Comissão Científica)
2017 - 2017 II International Congress in Health Sciences Research: Towards Innovation and Entrepreneurship – Trends in Biotechnology for Biomedical Applications (2017 - 2017)
Congresso (Membro da Comissão Organizadora)
2013 - 2013 8º National meeting of Chromatography (2013 - 2013)
Congresso (Membro da Comissão Organizadora)
2013 - 2013 8º National meeting of Chromatography (2013 - 2013)
Congresso (Membro da Comissão Científica)

Participação em evento

Descrição da atividade
Tipo de evento
Nome do evento
Instituição / Organização
2022 - Atual Research Marathon, UNIGHT in the UNITA edition of the European Researchers' Night
Encontro
European Researchers' Night
Universidade da Beira Interior, Portugal
2022 - Atual round table - working on cancer therapies
Mesa-redonda
Dia Internacional do cancro
Universidade da Beira Interior, Portugal

Júri de grau académico

Tema
Tipo de participação
Nome do candidato (Tipo de grau)
Instituição / Organização
2023/10 Plasmid DNA-loaded calcium phosphate functional nanocomplexes: innovative approach against cervical cancer
Orientador
Matilde Bogalheiro Costa (Mestrado)
Universidade da Beira Interior, Portugal
2023/10 Potential inhibitors of the HPV E6 oncoprotein for personalized cervical cancer therapy
Orientador
Carolina Alves Ribeiro (Mestrado)
Universidade da Beira Interior, Portugal
2023/10 Formulation and Characterization of taxifolin delivery systems as anti-Human papillomavirus drug
Orientador
Miguel Leite Neto (Mestrado)
Universidade da Beira Interior, Portugal
2023/10 The role of the glucosensing receptor TAS1R3 in glioblastoma, breast, and colorectal cancer hallmarks
Arguente principal
Inês Rosado de Matos (Mestrado)
Universidade da Beira Interior, Portugal
2023/10 Simvastatin Formulations on Tumor Cell Proliferation and Viability
Arguente principal
Maria Carolina Martins Gaspar (Mestrado)
Universidade da Beira Interior, Portugal
2023/10 Characterization of the Inhibition Kinetics of the Membrane isoform of the Catechol-O-methyltransferase enzyme
Arguente principal
Adriana Esteves Oliveira (Mestrado)
Universidade da Beira Interior, Portugal
2023/05 Unveiling Biopathways from the Biosynthesis Towards the Purification of STEAP1 Protein New Insights on Stability, Structure, and Function
Arguente
Jorge Daniel Barroca Ferreira (Doutoramento)
Universidade da Beira Interior, Portugal
2023/03/14 Physical and Chemical Parameters Screening for Co-Crystallization of rS -COMT-entacapone
Arguente principal
Pedro Daniel de Jesus Conde Fernandes (Mestrado)
Universidade da Beira Interior, Portugal
2023/03 Biomanufacturing of DNA-origami nanostructures for bio-imaging
Arguente principal
Ana Rita da Silva Santos (Doutoramento)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2023/02/23 In vitro evaluation of the microRNA-29 delivery to lung cancer cells
Arguente principal
Maria Carolina Martins Marques (Mestrado)
Universidade da Beira Interior, Portugal
2023 Application of membranes for clarification and/or purification of a minicircular DNA vector
Orientador
Daniela Maria Domingos de Carvalho (Mestrado)
Universidade da Beira Interior, Portugal
2022 Obtaining a minicircular DNA vaccine against COVID-19
Orientador
Cathy Lopes Ventura (Mestrado)
Universidade da Beira Interior, Portugal
2022 Delivery of anti-HPV drugs to cervical cancer cells
Orientador
Miguel Rodrigues Ferreira (Mestrado)
Universidade da Beira Interior, Portugal
2021 Targeted delivery to dendritic cells: Functionalization of a minicircular DNA vector with ligands
Orientador
Ana Sofia Serra (Mestrado)
Universidade da Beira Interior, Portugal
2021 Chitosan-triphosphate-DNA nanoparticles for delivery of DNA vaccines
Orientador
Renato Pereira Nunes (Mestrado)
Universidade da Beira Interior, Portugal
2021 Optimization of chitosan-based nanosystems to deliver plasmid DNA vaccines
Orientador
Carlos Afonso Rodolfo (Mestrado)
Universidade da Beira Interior, Portugal
2020/11 Development of new Catechol-Omethyltransferase inhibitors
Arguente principal
Pedro Miguel da Cruz Vicente (Mestrado)
Universidade da Beira Interior, Portugal
2020/07 Biosynthesis and purification of minicircle DNA encoding pri-miR-375 for treatment of Human Papillomavirus infection
Orientador
Ana Margarida Cardoso Valério de Almeida (Doutoramento)
Universidade da Beira Interior, Portugal
2019/10/15 Application of gelan spheres to capture or purify a plasmid DNA vaccine
Orientador
Diana Vanessa Duarte Gomes (Mestrado)
Universidade da Beira Interior, Portugal
2019/10/12 Proteomic study of the therapeutic effect of a DNA vector on cervical cancer cells
Orientador
Adriana Resende Pinto (Mestrado)
Universidade da Beira Interior, Portugal
2019/10/11 In vitro evaluation of the synergistic effect of a multigenic vector of minicircular DNA in cervical cancer cells
Orientador
Diana Carvalho Pereira (Mestrado)
Universidade da Beira Interior, Portugal
2019 Biophysical study of therapeutic antibody adsorption in affinity chromatography
Arguente
Gonçalo Fradique Lopes da Silva (Doutoramento)
Universidade da Beira Interior, Portugal
2018/10/10 Multigenic minicircular DNA with potential therapeutic action in the cervical cancer
Orientador
Dalinda Isabel da Silva Eusébio (Mestrado)
Universidade da Beira Interior, Portugal
2018 Mitochondrial gene therapy: In vitro studies using a plasmid DNA (pDNA) transport system with affinity for mitochondria
Arguente principal
Ana Cristina Candeias Melo (Mestrado)
Universidade da Beira Interior, Portugal
2018 The impact of oxidative stress and redox systems on malaria
Arguente principal
Olavo Patrício de Almeida Miguel (Mestrado)
Universidade da Beira Interior, Portugal
2018 Design of an one-step platform purification of STEAP1 using Hydrophobic Interaction Chromatography
Arguente principal
Diogo José Pinheiro Monteiro (Mestrado)
Universidade da Beira Interior, Portugal
2017/10/06 Minicircle DNA vectors with potential therapeutic action in cervical cancer
Orientador
Joel Marques Alves (Mestrado)
Universidade da Beira Interior, Portugal
2017/10/04 Cervical cancer therapy: Obtaining of a plasmid DNA vector and target delivery to the tumor cells
Orientador
Pedro Almeida (Mestrado)
Universidade da Beira Interior, Portugal
2017/07/14 Biosynthesis, Isolation and Magnetization of gellan spheres for biorecognition of therapeutic His-tag proteins
Vogal
Joana Coelho (Mestrado)
Universidade da Beira Interior, Portugal
2017 Improvement of Steap 1 Biosynthesis from Pichia Pastoris X33 cells under an optimized feeding strategy
Arguente principal
Diana Rute Tavares Duarte (Mestrado)
2016/07/09 Purification of a plasmid DNA vaccine for prevention or treatment of cervical cancer
Orientador
Duarte Gonçalves Carapito (Mestrado)
Universidade da Beira Interior, Portugal
2016/07/06 Obtaining of a DNA vaccine to prevent or treat the cervical cancer
Orientador
Ana Rita Simões (Mestrado)
Universidade da Beira Interior, Portugal
2016 Biosynthesis and Isolation of Steap1 Protein From Escherichia Coli Cells Lysates
Arguente principal
João Pedro Vicente Pais (Mestrado)
Universidade dos Açores, Portugal
2016 Understanding The Interaction Between PDNA and Different Chromatographic Supports
Arguente principal
Gregory Silva Dutra (Mestrado)
2015/07/11 Obtaining a plasmid DNA vaccine against HPV
Orientador
Vanessa Andrade Figueiredo (Mestrado)
Universidade da Beira Interior, Portugal
2015/07/10 Application of a gellan gum support for recombinant proteins or nucleic acids partition
Orientador
Filipe dos Santos Frias (Mestrado)
Universidade da Beira Interior, Portugal
2015/07/08 Purification of a plasmid DNA vaccine against HPV
Orientador
Rita Isabel Mota Gaspar (Mestrado)
Universidade da Beira Interior, Portugal
2014/07/16 Purification of plasmid DNA HPV-16 E6/E7 vaccine and in vitro and in vivo immunogenicity evaluation
Orientador
Ana Margarida Cardoso Valério de Almeida (Mestrado)
Universidade da Beira Interior, Portugal
2014/07/14 Purification of supercoiled HPV-16 E6/E7 plasmid using a modified monolithic support
Orientador
Lúcia Filipa Alves Amorim (Mestrado)
Universidade da Beira Interior, Portugal
2014 Biorecognition by amino acid-based affinity chromatography for RNA purification
Arguente
Ana Rita Nunes Martins (Doutoramento)
Universidade da Beira Interior, Portugal
2013/07/06 Preparation of the HPV-16 E6/E7 DNA vaccine: purification with modified monoliths and in vitro evaluation
Orientador
Alexandra Sabrina Antunes Soares (Mestrado)
Universidade da Beira Interior, Portugal
2013/07/06 Development and optimization of a microRNA purification technology by affinity chromatography, using the concept of QbD
Vogal
Adriana Alexandra Fernandes Afonso (Mestrado)
Universidade da Beira Interior, Portugal
2013/07/05 Development of new chromatographic support based on gellan gum for pharmaceutical biomolecules isolation
Vogal
Armanda Isabel Carvalho Gonçalves (Mestrado)
2013 Novel affinity chromatography process for the purification of plasmid DNA using small aromatic molecules
Arguente
Catarina Caramelo Nunes (Doutoramento)
Universidade da Beira Interior, Portugal
2013 Purification of Recombinant human membrane COMT by ionic exchange chromatography
Arguente principal
Filipa Inês Feliciano Correia (Mestrado)

Arbitragem científica em conferência

Nome da conferência Local da conferência
2023/12 - Atual MicroBiotec’23 - Portuguese Congress of Microbiology and Biotechnology Covilhã
2019/11/14 - 2019/11/16 III International Congress in Health Sciences Research: Towards Innovation and Entrepreneurship – Trends in Aging and Cancer Universidade da Beira Interior
2019 - 2019 XIV Annual CICS-UBI Symposium
2017 - 2017 II International Congress in Health Sciences Research: Towards Innovation and Entrepreneurship – Trends in Biotechnology for Biomedical Applications
2017 - 2017 XII Annual CICS-UBI Symposium
2015 - 2015 X Annual CICS-UBI Symposium

Arbitragem científica em revista

Nome da revista (ISSN) Editora
2022 - Atual Cells
2022 - Atual ACS Applied Nano Materials
2022 - Atual Frontiers in Oncology
2022 - Atual Hindawi
2022 - Atual Artificial Cells, Nanomedicine and Biotechnology
2021 - Atual Life
2021 - Atual Biochemical Pharmacology
2021 - Atual Frontiers in Bioengineering and Biotechnology
2021 - Atual Materials Science & Engineering C
2021 - Atual Molecular Biotechnology
2021 - Atual Materials Today Bio
2021 - Atual International Journal of Nanomedicine
2021 - Atual Plants MDPI
2021 - Atual Biomolecules
2021 - Atual Pharmaceutics
2021 - Atual International Journal of Molecular Sciences
2020/03/01 - Atual Archives of Biochemistry and Biophysics (1096-0384) Elsevier
2020/02/05 - Atual Medicinal Research Reviews (1098-1128) Wiley (John Wiley & Sons)
2020/01/08 - Atual Electrophoresis (1522-2683) Wiley (John Wiley & Sons)
2020 - Atual IUBMB Life Wiley Online Library
2020 - Atual ImmunoTargets and Therapy
2020 - Atual Scientific Reports Nature
2020 - Atual Cancers MDPI
2020 - Atual International Journal of Environmental Research and Public Health MDPI
2019/10/03 - Atual Microorganisms (2076-2607) MDPI AG
2019/07/05 - Atual Pharmacological Reports (1734-1140) Elsevier
2019 - Atual Vaccines (2076-393X) MDPI AG
2019 - Atual Colloids and Surfaces B Biointerfaces (0927-7765) Elsevier
2019 - Atual Membranes (2077-0375) MDPI AG
2018 - Atual Chemical Engineering Journal (1385-8947) Elsevier
2018 - Atual Clarivate Analytics
2017 - Atual Separations (2297-8739) MDPI AG
2016 - Atual Process Biochemistry (1359-5113) Elsevier
2016 - Atual RSC Advances (2046-2069) The Royal Society of Chemistry
2016 - Atual Journal of Chromatography B (1570-0232) Elsevier
2016 - Atual Polymers (2073-4360) MDPI AG
2015 - Atual Chromatography (1348-3315) The Society for Chromatographic Sciences
2015 - Atual ACS Applied Materials & Interfaces (1944-8252) American Chemical Society
2015 - Atual Molecular Pharmaceutics (1543-8392) American Chemical Society
2014 - Atual Journal of Biotechnology (0168-1656) Elsevier
2013 - Atual Journal of Chromatography A (0021-9673) Elsevier
2013 - Atual Journal of Separation Science (1615-9314) Wiley (John Wiley & Sons)

Comissão de avaliação

Descrição da atividade
Tipo de assessoria
Instituição / Organização Entidade financiadora
2021 - Atual Evaluator of national projects of Incentive System for Research and Technological Development (SI I&DT).
Avaliador
Agência Nacional de Inovação SA

Curso / Disciplina lecionado

Disciplina Curso (Tipo) Instituição / Organização
2024/01 - Atual Como produzir uma vacina em laboratório? AJSTEAM Junior Academy (Curso de Especialização Tecnológica) Universidade da Beira Interior, Portugal
2024 - Atual DNA Vaccines: From Design to Approval DNA Vaccines: From Design to Approval (Curso de doutoramento (conclusão de unidades curriculares)) Universidade da Beira Interior, Portugal
2023/05 - Atual Medical Structural Biology Medical Structural Biology (Curso de doutoramento (conclusão de unidades curriculares)) Universidade da Beira Interior, Portugal
2022 - Atual Bioinformatics for “dummies” AJSTEAM Junior Academy Universidade da Beira Interior, Portugal
2021/02/10 - Atual Student Choice Elements Medicina (Licenciatura) Universidade da Beira Interior, Portugal
2018/02/10 - Atual Seminar - Biomedical Sciences Ciências Biomédicas (Licenciatura) Universidade da Beira Interior, Portugal
2017 - Atual Biotechnology and innovation Biotecnologia (Mestrado integrado) Universidade da Beira Interior, Portugal
2013 - Atual Bioprocess Design Biotecnologia e Bioengenharia
2013 - Atual Pharmaceutic Biotechnology Ciências Farmacêuticas e Biotecnologia
2013 - Atual Biotechnology Ciências Biomédicas, Bioengenharia, Bioquímica
2012 - Atual Training of Younger Investigators in Biologic security and Good laboratorial practices Bioquímica, Biotecnologia, Ciências Biomédicas, Bioengenharia, Medicina, Ciências Farmacêuticas
2019/11/10 - 2019/11/14 Pharmaceutical Biotechnology Pos Graduation Program in Biosciences and Biotechnology Apply to Pharmacy (Postgraduate Certificate) Universidade Estadual Paulista Júlio de Mesquita Filho - Câmpus de Araraquara, Brasil
2019 - 2019 Responsible researcher for the internship developed under SEP, Student Exchange Programme of IPSF UBIPharma (Mobility Department of pharmaceutical sciences students. Minicircle DNA vaccine against Human Papilloma Virus infection
2015 - 2019 Responsible researcher for the integrated internship on the program "Ciência Viva no Laboratório - Ocupação Científica de Jovens nas férias" “Evaluation of the potential therapeutic action of a minicircle DNA vector in cervical cancer” and “Data management and editing a digital lab notebook”
2015 - 2019 Responsible researcher for the internship developed under Scientific International Exchanges (LORE) of MedUBI (Mobility Department of medical students) Preparing of a DNA vaccine for the treatment of the cervical cancer
2017/02 - 2018/07 Biochemistry II Bioquímica; Ciências Biomédicas; Ciências Farmacêuticas; Biotecnologia Universidade da Beira Interior, Portugal
2017 - 2018 Protein Structure and Function Biotecnologia e Bioquímica Universidade da Beira Interior, Portugal
2017 - 2018 Cellular communication Bioquímica Universidade da Beira Interior, Portugal
2012 - 2012 Celular Biology Medicina
2009 - 2011 Analytical Methods II Bioquímica e Ciências Farmacêuticas
2008 - 2011 Cellular and molecular biology Bioquímica, Biotecnologia, Ciências Biomédicas e Ciências Farmacêuticas

Entrevista (jornal / revista)

Descrição da atividade Jornal / Forum
2023/06 A. Sousa will receive funding from RESTART program (50000€), promoted by the Foundation for Science and Technology for the development of the project “DryVac: Dry powder mannosylated minicircle DNA nanoVaccine against Cervical Cancer” UBI journal
2023/03/25 UBI, in partnership with Banco Santander, awarded Ângela Sousa, on the 8th of March, the Young Investigator Prize. The award-winning project is entitled "MIND: Multifunctional Innovative NanoDevice for lung cancer therapy" and proposes to study a new form of treatment for lung cancer, which avoids the use of chemotherapy and its negative effects, with other advantages such as cost reduction for the health system. Thus, Jornal Fórum went to listen to the award-winning. FÓRUM COVILHÃ
2023/03/09 A. Sousa received the “UBI/Santander young researcher award” with the project “MIND: Multifunctional Innovative NanoDevice for lung cancer therapy” in March 2023 UBI Journal
2023/01/13 A. Sousa was awarded from the Ignition Projects and Proofs of Concept within the scope of the INOVC+ project/UBI with the project “NanoINTer: Functionalized DNA Nanovaccine for Cervical Cancer Immunotherapy” UBI journal
2023 The project ONCOSENSING under the mentorship of Ângela Sousa was awarded (5000€) by 2023 edition of the PROMOVE Program of the “La Caixa” Foundation . UBI notícias
2023 A. Sousa was awarded with an Assistant Research position of institutional CEEC – UBI; with the project MIRACLE - Dry powder mannosylated MIniciRcle DNA nanovaccine against CervicaL cancEr” UBI notícias
2021/02/11 International Day of Women and Girls in Science - February 11 Testimony as an Investigator UBIPHarma
2020/08/22 Young doctoral student, under the guidance of researcher Ângela Sousa, is going to develop a vaccine against COVID-19. Jornal O Interior
2020/08/17 PhD student in Biomedicine at the University of Beira Interior, supervised by Ângela Sousa, received a research grant for the development of a preventive and therapeutic nanovaccine against COVID-19. Viva Serra Digital Magazine
2020/08/12 Young doctoral student at the University of Beira Interior, under the guidance of Ângela Sousa Researcher, won a doctoral scholarship to create a vaccine that, in addition to being preventive, also intends to be therapeutic. Diário de Notícias
2020/08/10 Covid-19: Student at the University of Beira Interior studies nanovaccine PAÍSAOMINUTO
2020/08/10 PhD scholarship to develop vaccine for COVID-19 JORNAL do FUNDÃO
2020/08/10 PhD scholarship to develop vaccine for COVID-19 beira.pt

Entrevista / Programa (rádio / tv)

Programa Tema
2024/01/26 - Atual RTP Marcas - ONCOSENSING - Awarded project from PROMOVE programe from La Caixa Fundation. ONCOSENSING - Awarded project from PROMOVE programe from La Caixa Fundation.
2021/03/09 - Atual tvi 24 A NANOVACCINE, DRUG AND TEST WITH RESULTS IN 45 MINUTES: THE FIGHT OF THE PORTUGUESE SCIENTIFIC COMMUNITY AGAINST COVID-19
2021/02/09 - Atual TV show "É ou NÃO É? - The Great Debate - RTP1 In Search of Immunity - Debate on the status of vaccination in Portugal.
2020/12/05 - Atual Flagrant Direct - Radio Cova da Beira UBI researchers are working on the creation of an innovative vaccine against COVID-19.
2020/11/21 - Atual TV show "Página 2" Project "Development of a minicircular DNA vaccine against COVID-19" was awarded a FCT PhD scholarship to student Dalinda Eusébio.
2020/11/20 - Atual Jornal das 8: "IMMUNITY - Great Report TVI" Innovative project "Development of a minicircular DNA vaccine against COVID-19" was awarded a FCT PhD scholarship to student Dalinda Eusébio and is being developed at CICS-UBI, Covilhã, coordinated by Ângela Sousa researcher.
2020/09/02 - Atual TV show "SIC Notícias" University of Beira Interior is developing a vaccine against Covid-19

Membro de comissão

Descrição da atividade
Tipo de participação
Instituição / Organização
2022/12/05 - 2022/12/07 Scientific Board of "Global Conference on Vaccines Research and Development" Seville, Spain
Membro
2019/05/14 - 2019/05/16 Member of Scientific Committee of the "III International Congress in Health Sciences Research: Towards Innovation and Entrepreneurship – Trends in Aging and Cancer”
Membro
2019/05/14 - 2019/05/16 Member of Awards Committee of the III International Congress in Health Sciences Research: Towards Innovation and Entrepreneurship – Trends in Aging and Cancer” To evaluate the best poster presentation
Membro
2017/07/06 - 2017/07/07 Member of the Awards Committee of the XII Annual CICS-UBI Symposium to evaluate the best short and oral communication of master and doctoral students
Membro
2017/05/17 - 2017/05/20 Member of Organizing Committee and Member of Awards Committee of the II International Congress in Health Sciences Research: Towards Innovation and Entrepreneurship "Trends in Biotechnology for Biomedical Applications" To evaluate the best oral communication.
Membro
2015/07/06 - 2015/07/07 Member of the Awards Committee X Annual CICS-UBI Symposium to evaluate the best oral communication of doctoral students.
Membro
2013/12/02 - 2013/12/04 Member of Scientific Committee of the 9th Oral section of the "8º National meeting of Chromatography"
Membro

Outro júri / avaliação

Descrição da atividade Instituição / Organização
2024/01 - Atual Jury of a Doctoral Fellowship in the scope of Circular Economy, project “A new life in Citrus peels: from an agroindustrial waste to a promising antifungal biotherapeutic”, funded by the Foundation for Science and Technology (FCT) under the Collaboration Protocol for Financing the Pluriannual Plan of the PhD research fellowships, celebrated between FCT and the University of Beira Interior, within the scope of the consortium Universitas Montium (UNITA). Universidade da Beira Interior, Portugal
2022/08 - Atual Jury of six Research Initiation Scholarships for students enrolled in Masters or Integrated Masters (BII) within the scope of the Research Project entitled Multidisciplinary Experience in Research to fight Cancer and Infection (MERCI) Project, funded by special support "Summer with Science" supported by national funds through the budget of the Foundation for Science and Technology (FCT), taking place at the Health Sciences Research Centre of the University of Beira Interior (2022). Universidade da Beira Interior, Portugal
2021 - Atual Jury of six Research Initiation Scholarships for students enrolled in Masters or Integrated Masters (BII) within the scope of the Research Project entitled Multidisciplinary Experience in Research to fight Cancer and Infection (MERCI) Project, funded by special support “Summer with Science” supported by national funds through the budget of the Foundation for Science and Technology (FCT), taking place at the Center for Research in Health Sciences of the University of Beira Interior (2021). Universidade da Beira Interior, Portugal
2020 - Atual Jury of a master fellowship from the project “PAMI - Portuguese Initiative in Additive Manufacturing (CENTRO-01-0145-FEDER-022158)” co-financed by the Foundation for Science and Technology, IP and the European Regional Development Fund (ERDF), within the scope Portugal 2020 Program, through the Centro Regional Operational Program (CENTRO 2020) (2020). Instituto Politécnico de Leiria Centro para o Desenvolvimento Rápido e Sustentado de Produto, Portugal
2013 - Atual Jury of a master fellowship from the project “Biosynthesis, purification and biointeraction of human soluble catechol-O-methyltransferase protein with inhibitors of Parkinson diseases”, Ref: EXPL/BBB-BQB/0960/2012, financed by the Portuguese Science and Technology Foundation (2013) Universidade da Beira Interior, Portugal
2013 - Atual Jury of a master fellowship from the project “Technologies for Purification and controlled release of biopharmaceuticals to be applied in age—related diseases”, Ref: CENTRO-07-ST24-FEDER-002014, financed by the Center Regional Operational Programme 2007-2013 QREN (2013) Universidade da Beira Interior, Portugal
2013 - Atual Jury of a master fellowship from the project “Bioseparation and targeted delivery of pre-miR-29 for Alzheimer disease application”, Ref: EXPL/BBB-BIO/1056/2012, financed by the Portuguese Science and Technology Foundation (2013) Universidade da Beira Interior, Portugal
2013 - Atual Jury of a master fellowship from the project “Hrmonal and Inflammatory basis of age-related diseases”, Ref: CENTRO-07-ST24-FEDER-002015, funded by the Centro Regional Program 2007-2013 QREN (Prograna “Mais Centro”) (2013) Universidade da Beira Interior, Portugal
2013 - Atual Jury for the acquisition of an orbital shaker from the project: Center Regional Operational Program – More Center: System Support for Scientific and Technological Infrastructures (Centro-ICT-2013-05) Universidade da Beira Interior, Portugal
2013 - Atual Jury for the acquisition of an Chromatographic System from the project: Center Regional Operational Program – More Center: System Support for Scientific and Technological Infrastructures (Centro-ICT-2013-05) Universidade da Beira Interior, Portugal
2013 - Atual Jury for the acquisition of Software from Proteomics laboratory from the project: Center Regional Operational Program – More Center: System Support for Scientific and Technological Infrastructures (Centro-ICT-2013-05) Universidade da Beira Interior, Portugal
Distinções

Prémio

2023 Ignition Projects and Proofs of Concept within the scope of the INOVC+ project/UBI with the project NanoINTer: Functionalized DNA Nanovaccine for Cervical Cancer Immunotherapy
Universidade da Beira Interior, Portugal
2023 UBI/Santander young researcher award, with the project MIND: Multifunctional Innovative NanoDevice for lung cancer therapy
Banco Santander Portugal, Portugal

Universidade da Beira Interior, Portugal
2023 Younger investigator Award - Flash Communication: D. Gomes, C. Ribeiro, V. Brito, S. Silvestre, L. Passarinha, A. Sousa. “Exploring synthetic compounds as HPV E6 protein inhibitors for cervical cancer management” AFFINITY 2023, Lisboa, Portugal
2023 Best SPBf Flash Communication: D. Gomes, V. Kumar, S. Silvestre, L. Passarinha, A. Sousa. “Targeting HPV E6 protein: computational and experimental analysis of synthetic compounds” 3rd Meeting of Young Biophysicists, I3S Porto, Portugal
2023 The project ONCOSENSING under the mentorship of Ângela Sousa was awarded (5000€) by 2023 edition of the PROMOVE Program of the “La Caixa” Foundation
Fundação 'la Caixa', Portugal
2023 Joint publication merit award (500 €) from Faculty of Sciences of University of Beira Interior (doi: 10.3390/vaccines10091560)
2023 Oncosensing; Programme PROMOVE
Fundação 'la Caixa', Portugal
2022 Best Oral Presentation of the workshop internacional Path/mVACCIL/IL2BioPro of the CICECO, University of Aveiro
Universidade de Aveiro CICECO, Portugal
2022 Best Oral communication of the Biopartitioning & Purification conference (BPP), Aveiro, Portugal
Universidade de Aveiro CICECO, Portugal

Outra distinção

2015 Best Oral Communication: Polytechnic Institute of Guarda
Instituto Politécnico da Guarda, Portugal
2015 Best Oral Communication in X Annual CICS-UBI Symposium of Health Sciences Research Centre of University of Beira Interior
2015 Best Poster Presentation at I Congress in Health Sciences Research: Towards Innovation and Entrepreneurship - Trends in Endocrinology and Neurosciences
2014 Best "Short Communication" in IX CICS Simposium, of Health Sciences Research Centre of University of Beira Interior
2013 Best poster presentation in the session "Bioprocess Engineering" of the congress Micro Biotech'13, Aveiro
2011 First award of the scientific publication with higher impact factor in the Health Sciences research center
2009 Honourable mention of the Roche Younger Investigator Award 2009
Roche, Hungria