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Pedro Prudêncio completed the Ph.D. in Ciências Biomedicas in 2020/07/23 by Universidade do Algarve Departamento de Ciências Biomédicas e Medicina, Mestrado in Mestrado Integrado em Engenharia Biologica in 2010 by Universidade do Algarve and Licenciatura in Engenharia Biotecnológica in 2007 by Universidade do Algarve. He is a Postdoc in Instituto de Medicina Molecular JLA at Faculdade de Medicina da Universidade de Lisboa. Published 14 articles in journals. Supervised 1 MSc dissertation. Participated as Post-doc in 1 project and collaborates on 3 other projects. Works in the area of Molecular Cell Biology with emphasis on Gene expression regulation more specifically Splicing regulation during RNA transcription. He has expertise in a vast type of techniques, namely: plasmid cloning techniques; CRISPR-Cas9 gene editing; RNA/DNA extraction from several cell types and tissues; preparation of libraries for RNA-seq and NET-seq; protein blot analysis; Co-immunoprecipitation of proteins; Chromatin immunoprecipitations; immunohistochemistry and the skills handling cell cultures and Drosophila flies
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Identification

Personal identification

Full name
Pedro Prudêncio

Citation names

  • Prudêncio, Pedro

Author identifiers

Ciência ID
7B1C-9F36-22C8
ORCID iD
0000-0003-1390-8491
Google Scholar ID
https://scholar.google.com/citations?hl=pt-PT&user=NnJJEewAAAAJ

Knowledge fields

  • Natural sciences - Biological Sciences - Cell Biology

Languages

Language Speaking Reading Writing Listening Peer-review
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Education
Degree Classification
2016/07/01 - 2020/07/23
Concluded
 Ciências Biomedicas (Doutoramento)
Universidade do Algarve Departamento de Ciências Biomédicas e Medicina, Portugal
"Pre-mRNA splicing and regulation of gene expression after egg fertilization" (THESIS/DISSERTATION)
 Muito Bom, com Distinção e Louvor
2010
Concluded
 Mestrado Integrado em Engenharia Biologica (Mestrado)
Universidade do Algarve, Portugal
"Differential requirements of aPKC activity within different epithelial tissues" (THESIS/DISSERTATION)
 15
2007
Concluded
 Engenharia Biotecnológica (Licenciatura)
Universidade do Algarve, Portugal
"Detecção de Ilarvirus por RT-PCR Multiplex em Real time" (THESIS/DISSERTATION)
 13
Affiliation

Science

Category
Host institution 		Employer
2020/01/08 - Current Postdoc (Research) Instituto de Medicina Molecular, Portugal
Instituto de Medicina Molecular, Portugal

Others

Category
Host institution 		Employer
2015/12/01 - 2020/02/10 PhD student Instituto de Medicina Molecular, Portugal
2010/04/01 - 2015/11/30 Graduate Researcher Instituto Gulbenkian de Ciência, Portugal
2008/01/01 - 2010/04/01 Master student Instituto Gulbenkian de Ciência, Portugal
2007/02/01 - 2007/11/01 Laboratory Technician Instituto Gulbenkian de Ciência, Portugal
Projects

Grant

Designation Funders
2022/01/01 - 2024/12/31 Mutação BRCA2 fundadora Portuguesa: impacto na transcrição e identidade de células estaminais Impact of the Portuguese BRCA2 founder mutation on transcription and tissue stem cell identity
PTDC/MED-ONC/3921/2021
Researcher
Instituto de Medicina Molecular, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2023/01/01 - 2024/06/30 Mapping the spatial organization of co-transcriptional splicing
2022.01836.PTDC
Principal investigator
Instituto de Medicina Molecular, Portugal
Ongoing
2022/01/01 - 2023/06/30 A new mechanism of nuclear compartmentalization in meiosis: from basic to clinical research in male infertility
EXPL/MEC-AND/0676/2021
Researcher
Instituto de Medicina Molecular, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2022/01/01 - 2023/06/30 Inativação do Cromossoma X no Lúpus Eritematoso Sistémico X-Chromosome Inactivation in Systemic Lupus Erythematosus
EXPL/MED-OUT/1517/2021
Researcher
Instituto de Medicina Molecular, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2018/09/29 - 2021/09/28 O despertar do oócito: reactivação transcricional após um estado quiescente prolongado.
PTDC/BIA-BID/28441/2017
Integration into Research Grant Fellow
Universidade do Algarve, Portugal

Fundação Calouste Gulbenkian, Portugal

Universidade do Porto, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2020/09/22 - 2021/01/31 Promoção do avanço significativo do conhecimento na área de medicina de precisão e sua translação para benefício da sociedade
SAICTPAC/0021/2015
Post-doc
Instituto de Medicina Molecular, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2015/12/01 - 2019/12/01 Pre-mRNA splicing and regulation of gene expression after egg fertilization
SFRH/BD/109689/2015
PhD Student Fellow
Universidade do Algarve Departamento de Ciências Biomédicas e Medicina, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2008/02/01 - 2011/03/31 Caracterização dos mecanismos moleculares responsáveis pela indução de transcrição zigótica após a fertilização do oócito.
PTDC/BIA-BCM/69256/2006
Integration into Research Grant Fellow
Fundação Calouste Gulbenkian, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2008/01/01 - 2010/12/31 Caracterização da segregação da linha germinal durante o desenvolvimento embrionário de Drosophila melanogaster
PPCDT/DG/BIA/82013/2006
Integration into Research Grant Fellow
Fundação Calouste Gulbenkian, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded

Contract

Designation Funders
2022/02/06 - 2022/12/31 Cardiac Splicing as a Therapeutic Target
21CVD02
Post-doc
Instituto de Medicina Molecular, Portugal
 Fondation Leducq
Ongoing
Outputs

Publications

Conference poster
  1. Prudêncio, Pedro. "Dynamics of anti-sense transcription during iPSC differentiation". Paper presented in Cell Symposia – “The conceptual power of single cell biology”, 2023.
  2. Prudêncio, Pedro; Savisaar, Rosina; Rebelo, Kenny; Martinho, Rui Gonçalo; Carmo-Fonseca, Maria. "The timing of co-transcriptional splicing in Drosophila embryos.". Paper presented in The 26th Annual Meeting of the RNA Society - RNA 2021, 2021.
  3. Prudêncio, Pedro; Savisaar, Rosina; Rebelo, Kenny; Martinho, Rui; Carmo-Fonseca, Maria. "“Immediate” co-transcriptional splicing in Drosophila is associated to S5P of the Pol II CTD". Paper presented in Splicecon 2021 Symposium. Virtual, 2021.
  4. Prudêncio, Pedro; Savisaar, Rosina; Rebelo K.; Martinho, Rui Gonçalo; Carmo-Fonseca, Maria. "Impact of gene architecture on pervasive transcription in Drosophila embryos.". Paper presented in EMBO | EMBL Symposium: The Complex Life of RNA. Virtual., 2020.
  5. Prudêncio, Pedro; Savisaar, Rosina; Rebelo K.; Martinho, Rui Gonçalo; Carmo-Fonseca, Maria. "Links between RNA Pol II pausing and immediate splicing.". Paper presented in International Meeting of the Portuguese Society of Genetics. Universidade do Algarve Faro, Portugal, 2020.
  6. Prudêncio, Pedro; Rebelo K.; Martinho, Rui Gonçalo; Carmo-Fonseca, Maria. "Genome-wide analysis of the nascent RNApolII transcriptome during Drosophila development. (short talk + poster)". Paper presented in EMBO|EMBL Symposium The Complex Life of mRNA. Heidelberg, Germany, 2018.
  7. Prudêncio, Pedro; Guilgur, Leonardo Gaston; Lisekova D.; Sobral, Daniel; Martinho, Rui Gonçalo. "Avoidance of premRNA splicing during Drosophila early embryonic development.". Paper presented in 2nd Meeting Portuguese Society for Development Biology. Lisboa, Portugal, 2013.
  8. Prudêncio, Pedro; Guilgur, Leonardo Gaston; Lisekova D; Sobral, Daniel; Martinho, Rui Gonçalo. "Developmental constraints to splicing in early Drosophila embryo.". Paper presented in 7th Annual Meeting for Gulbenkian Students (AMeeGuS). Seia, Portugal, 2013.
  9. Prudêncio, Pedro; Guilgur, Leonardo Gaston; Lisekova D; Sobral, Daniel; Martinho, Rui Gonçalo. "Developmental constraints to pre-mRNA splicing during Drosophila early embryogenesis". Paper presented in EMBO|EMBL Symposium The Complex Life of mRNA. Heidelberg, Germany, 2012.
  10. Prudêncio, Pedro; Guilgur, Leonardo Gaston; Lisekova D.; Ferreira, Tania; Martinho, Rui Gonçalo. "Drosophila aPKC is required for mitotic spindle orientation during symmetric division of epithelial cells". Paper presented in 22nd European Drosophila Research Conference. Lisbon, Portugal, 2011.
  11. Prudêncio, Pedro; Guilgur, L.G.; Lisekova D.; Ferreira, Tania; Martinho, Rui Gonçalo. "Tissue specific requirements of Drosophila NTC complex in pre-mRNA splicing.". Paper presented in Eukaryotic mRNA Processing meeting, Cold Spring Harbor Laboratory, 2011.
Journal article
  1. Rion B. Correia; M. Almeida, Joana; Wyrwoll, Margot J.; Irene Julca; Sobral, Daniel; Misra, Chandra Shekhar; Guilgur, Leonardo G.; et al. Corresponding author: Navarro-Costa, Paulo. "The conserved transcriptional program of metazoan male germ cells uncovers ancient origins of human infertility". bioRxiv (2022):
    Submitted • https://doi.org/10.1101/2022.03.02.482557
  2. Prudêncio, Pedro; Savisaar, Rosina; Rebelo, Kenny; Martinho, Rui Gonçalo; Carmo-Fonseca, Maria. "Transcription and splicing dynamics during early Drosophila development". RNA 28 2 (2022): 139-161. http://dx.doi.org/10.1261/rna.078933.121.
    10.1261/rna.078933.121
  3. Rathore, Om Singh; Silva, Rui D.; Ascensão-Ferreira, Mariana; Matos, Ricardo; Carvalho, Célia; Marques, Bruno; Tiago, Margarida N.; et al. "NineTeen Complex-subunit Salsa is required for efficient splicing of a subset of introns and dorsal–ventral patterning". RNA 26 12 (2020): 1935-1956. http://dx.doi.org/10.1261/rna.077446.120.
    10.1261/rna.077446.120
  4. Prudêncio, Pedro. "Analysis of Mammalian Native Elongating Transcript sequencing (mNET-seq) high-throughput data". Methods (2019): http://dx.doi.org/10.1016/j.ymeth.2019.09.003.
    10.1016/j.ymeth.2019.09.003
  5. Prudêncio, Pedro. "The Trithorax group protein dMLL3/4 instructs the assembly of the zygotic genome at fertilization". EMBO reports (2018): http://dx.doi.org/10.15252/embr.201845728.
    10.15252/embr.201845728
  6. Rathore, O.S.; Faustino, A.; Prudêncio, P.; Van Damme, P.; Cox, C.J.; Martinho, R.G.. "Absence of N-terminal acetyltransferase diversification during evolution of eukaryotic organisms". Scientific Reports 6 (2016): http://www.scopus.com/inward/record.url?eid=2-s2.0-84958074128&partnerID=MN8TOARS.
    10.1038/srep21304
  7. Navarro-Costa, P.; McCarthy, A.; Prudêncio, P.; Greer, C.; Guilgur, L.G.; Becker, J.D.; Secombe, J.; Rangan, P.; Martinho, R.G.. "Early programming of the oocyte epigenome temporally controls late prophase i transcription and chromatin remodelling". Nature Communications 7 (2016): http://www.scopus.com/inward/record.url?eid=2-s2.0-84981510292&partnerID=MN8TOARS.
    10.1038/ncomms12331
  8. Martinho RG; Guilgur LG; Prudêncio P. "How gene expression in fast-proliferating cells keeps pace.". (2015): http://europepmc.org/abstract/med/25823409.
    10.1002/bies.201400195
  9. Prudêncio, Pedro; Guilgur, Leonardo. "Protein Extraction from Drosophila Embryos and Ovaries". BIO-PROTOCOL 5 9 (2015): http://dx.doi.org/10.21769/bioprotoc.1459.
    10.21769/bioprotoc.1459
  10. Prudêncio, Pedro; Guilgur, Leonardo. "FLP/FRT Induction of Mitotic Recombination in Drosophila Germline". BIO-PROTOCOL 5 9 (2015): http://dx.doi.org/10.21769/bioprotoc.1458.
    10.21769/bioprotoc.1458
  11. Ferreira, Tania; Prudêncio, Pedro; Martinho, Rui Gonçalo. "Drosophila protein kinase N (Pkn) is a negative regulator of actin-myosin activity during oogenesis.". (2014): http://europepmc.org/abstract/med/25131196.
    10.1016/j.ydbio.2014.08.008
  12. Guilgur, Leonardo Gaston; Prudêncio, Pedro; Sobral, D.; Liszekova, D.; Rosa, A.; Martinho, Rui Gonçalo. "Requirement for highly efficient pre-mRNA splicing during Drosophila early embryonic development". eLife 2014 3 (2014): http://www.scopus.com/inward/record.url?eid=2-s2.0-84899657623&partnerID=MN8TOARS.
    10.7554/eLife.02181.001
  13. Alves E; Henriques BJ; Rodrigues JV; Prudêncio, Pedro; Rocha H; Vilarinho L; Martinho RG; Gomes CM. "Mutations at the flavin binding site of ETF:QO yield a MADD-like severe phenotype in Drosophila.". (2012): http://europepmc.org/abstract/med/22580358.
    10.1016/j.bbadis.2012.05.003
  14. Guilgur LG; Prudêncio, Pedro; Ferreira, Tania; Pimenta-Marques AR; Martinho, Rui Gonçalo. "Drosophila aPKC is required for mitotic spindle orientation during symmetric division of epithelial cells.". (2012): http://europepmc.org/abstract/med/22223679.
    10.1242/dev.071027
Thesis / Dissertation
  1. Prudêncio, Pedro. "Pre-mRNA splicing and regulation of gene expression after egg fertilization". PhD, Universidade do Algarve Departamento de Ciências Biomédicas e Medicina, 2020.
  2. Prudêncio, Pedro António Pereira. "Differential requirements of aPKC activity within different epithelial tissues". Master, 2009. http://hdl.handle.net/10400.1/5508.
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2021/12/03 Splicing: astonishing facts and open questions. Seminar for Mestrado em Biologia Molecular e Microbiana,
Faculdade de Medicina e Ciências Biomédicas da Universidade do Algarve (Faro)
2020/03/09 Links between RNA Pol II Pausing and Immediate Splicing. iMM Scientific Retreat
Instituto de Medicina Molecular João Lobo Antunes (Lisboa, Portugal)
2018/10/03 Genome-wide analysis of the nascent RNApolII transcriptome during Drosophila development. (short talk + poster) EMBO|EMBL Symposium The Complex Life of mRNA
EMBO|EMBL (Heidelberg, Germany)
2018/09/08 Genome-wide analysis of the nascent RNApolII transcriptome during Drosophila development Portuguese Drosophila meeting.
(Tomar, Portugal)

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2020/09/01 - 2021/12/21 The role of cyclin-dependent kinases (CDKs) on co-transcriptional splicing through phosphorylation of the RNA Pol II carboxyl-terminal domain (CTD).
Supervisor
 Ciências Biofarmacêuticas (Master)
Universidade de Lisboa Faculdade de Farmácia, Portugal

Course / Discipline taught

Academic session Degree Subject (Type) Institution / Organization
2023/11 - 2023/11/16 "Junk code: The key for life complexity and diversity" Seminar for Master program in Biologia Molecular e Microbiana Master program in Biologia Molecular e Microbiana Universidade do Algarve Departamento de Ciências Biomédicas e Medicina, Portugal
2020/09/14 - 2020/09/25 Laboratory practical course for medical students at the University of Lisbon Medical School Medicina (Licenciatura) Universidade de Lisboa Faculdade de Medicina, Portugal

Mentoring / Tutoring

Topic Student name
2021/01/12 - 2021/02/04 Generation of APEX2 fusion proteins by CRISPR-Cas9 genome editing to study co-transcriptional splicing Júlia Canning Clode Castro Neves
2018/01/01 - 2018/04/20 project CanSat Portugal 2018 Bernardo Alves and Francisco Alves
2016/09/01 - 2017/09/01 Mestrado, Splicing kinetics revealed by nascent transcript sequencing. Kenny Patrick Figueira Rebelo
Distinctions

Award

2020 Best Poster
Sociedade Portuguesa de Genética Humana – SPGH, Portugal

Other distinction

2018 Travel grant
EMBO|EMBL , Germany