Matthew Guy Holt (3816-811A-DB66)

Curriculum Vitae

Matthew Guy Holt

Matthew Holt is the ERA Chair Holder for Neural Cell Biology (NCBio) and vice director of the Neurobiology and Neurologic Disorders integrative program at i3S, University of Porto. He is also head of the NCBio academic-industrial hub for northern Portugal. Dr. Holt holds a Ph.D. in neuroscience from the MRC Laboratory of Molecular Biology (2002); a post-doc from the Max Planck Institute for Biophysical Chemistry (2011) and established his own research group at KU Leuven in 2012. In 2022, Dr. Holt was appointed NCBio ERA Chair Holder at i3S. He has 20 years of scientific experience, particularly in the field of biochemistry (transcriptomics and proteomics) and cell biology. His work has been published in 59 peer-reviewed papers (H index = 35, >8500 citations; Google Scholar), often in high impact factor journals, including Cell, Nature Neuroscience and Nature Immunology. He has written 2 book chapters. His research aims to understand how astrocytes contribute to synapse formation and function. Work from Dr. Holt’s lab (amongst others) has been instrumental in establishing astrocytes as a highly molecularly heterogeneous cell type with implications for local circuit function, which he is currently following up using a range of technologies from advanced proteomics through to electrophysiology. Dr. Holt has attracted > €5M in 18 projects as Principal Investigator and > €11M in 15 projects as co-PI. He supervised/has supervised 10 Ph.D. candidates; 11 M.Sc. students; 7 B.Sc. students and has been an opponent in 31 Ph.D. thesis committees. He has given 50 invited talks at international conferences and has been awarded several prizes, including the prestigious ERC Consolidator Grant (2011) and the ERC Proof-of-Concept (PoC) Grant (2015). Dr. Holt has established an international reputation reflected by his election to the editorial board of the journal Glia, which is the main specialist journal in his field. In addition, he is a regular reviewer for high-impact, prestigious journals including (but not limited to): Cell, Nature, Nature Neuroscience, EMBO Journal, EMBO Molecular Medicine. His expertise is also sought as a regular reviewer for grant agencies including the ERC, and as an evaluator of scientific departments/institutes (such as Collège de France). Dr. Holt is co-founder of Aila Biotech, a spin-off company based on the considerable success of AAV-delivered IL2 in ameliorating traumatic brain injury (TBI), stroke and multiple sclerosis, which was published in Nature Immunology (Yshii et al, 2022). As a founder member, Dr. Holt has a place on the board of directors and acts as a science advisor. He also serves as scientific advisor to Verge Genomics, Janssen Pharma, Sirion Biotech and is inventor of 3 patents.

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Identification

Personal identification

Full name: Matthew Guy Holt

Citation names

Holt, Matthew
Matthew G. Holt

Author identifiers

Ciência ID: 3816-811A-DB66
ORCID iD: 0000-0002-8958-4027
Researcher Id: AAJ-1813-2020
Scopus Author Id: 35775275100

Telephones

Telephone

226074900 (Professional)

Addresses

Instituto de Biologia Molecular e Celular e Instituto de Investigação e Inovação em Saúde (i3S). Rua Alfredo Allen 208, 4200-135, Porto, Porto, Portugal (Professional)

Websites

https://ncbio.i3s.up.pt/ (Professional)
https://www.i3s.up.pt/research-group.php?groupid=201 (Professional)
https://twitter.com/AstrocytesRus (Social media)

Knowledge fields

Medical and Health Sciences - Basic Medicine - Neurosciences

Languages

Language	Speaking	Reading	Writing	Listening	Peer-review
English (Mother tongue)

Education

	Degree	Classification
1998 - 2002 Concluded	Neuroscience (Doctor of Philosophy) MRC Laboratory of Molecular Biology, United Kingdom
1994 - 1998 Concluded	Biochemistry (Bachelor) University of Liverpool, United Kingdom	1st Class Honors

Affiliation

Science

	Category Host institution	Employer
2022 - Current	Coordinating Researcher (Research)	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal

2012 - 2022	Principal Investigator (Research)	VIB KU Leuven Center for Brain & Disease Research, Belgium

2003 - 2011	Postdoc (Research)	Max-Planck-Institut für Multidisziplinäre Naturwissenschaften, Germany

2002 - 2003	Postdoc (Research)	MRC Laboratory of Molecular Biology, United Kingdom

Teaching in Higher Education

	Category Host institution	Employer
2024 - Current	Lecturer (University Teacher)	Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
	Universidade do Porto, Portugal
2023 - Current	Lecturer (University Teacher)	Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
	Universidade do Porto, Portugal
2023 - 2023	Lecturer (University Teacher)	Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
	Universidade do Minho, Portugal
2012 - 2022	Assistant Professor (University Teacher)	Associatie KU Leuven, Belgium

Others

	Category Host institution	Employer
2023/01/01 - Current	Vice-Chair for Neurobiology and Neurologic Disorders Program at the i3S	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2022/02/01 - Current	Head NCBio Academic-Industrial Hub for Portugal	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal

Projects

Grant

	Designation	Funders
2021/01 - 2026/12	Unlocking excellence in research and innovation in neurobiology and neurological disorders at IBMC/i3S 951923 Principal investigator	European Commission
2022 - 2025	Astrocyte dysfunctions in Phelan-McDermid syndrome: from mechanisms towards new therapeutic strategies EURON_NDD-003 / SHANKAstro Co-Principal Investigator (Co-PI)	European Commission
2022 - 2025	Investigating the role of astrocytes and noradrenaline as critical regulators of neuronal circuit excitability G0C7922N Co-Principal Investigator (Co-PI)	Fonds Wetenschappelijk Onderzoek Vlaanderen
2021 - 2024	Investigating the contribution of astrocytes to experience-dependent cortical plasticity G080821N Co-Principal Investigator (Co-PI)	Fonds Wetenschappelijk Onderzoek Vlaanderen
2021 - 2024	Cell subtype-specific neuron-glia contributions to cortical plasticity C14/20/071 Co-Principal Investigator (Co-PI)	Associatie KU Leuven
2021 - 2024	Cell subtype-specific neuron-glia contributions to cortical plasticity C14/20/071	Katholieke Universiteit Leuven
2020 - 2024	Integration of complementary, high throughput single-cell 'omics with spatial resolution I005920N Co-Principal Investigator (Co-PI)	Fonds Wetenschappelijk Onderzoek Vlaanderen
2022 - 2022	Confronting dementia in the front line: the role of astrocytes 2020/0020 Principal investigator	Stichting Alzheimer Onderzoek
2018 - 2022	Mass cytometry by time-of-flight: next-generation flow cytometry for multiplexed single cell analysis I007418N Co-Principal Investigator (Co-PI)	Fonds Wetenschappelijk Onderzoek Vlaanderen
2018 - 2022	Single-cell 'omics in high throughput and at spatial resolution I001818N	Fonds Wetenschappelijk Onderzoek Vlaanderen
2020/01 - 2021/12	Treating cognitive decline by targeting energy production in the central nervous system CELSA/19/036 Principal investigator	Katholieke Universiteit Leuven
2020 - 2021	Decoding the mechanisms of neuronal wiring: the clustered protocadherin system Internal Funding Principal investigator	Vlaams Instituut voor Biotechnologie
2020 - 2021	Validation of traumatic brain injury targets with large-scale spatial transcriptomics Internal Funding Principal investigator	Vlaams Instituut voor Biotechnologie
2019/01 - 2020/12	Study of the Disease Modifying Properties of an Optimized Vectored Immunoprophylaxis Approach for Alzheimer's 2018/29 Principal investigator	Stichting Alzheimer Onderzoek
2019 - 2020	Astrocyte driven recovery of neuronal loss in Alzheimer's disease brain 20180024 Principal investigator	Stichting Alzheimer Onderzoek
2016 - 2020	Development of a technology platform for next-generation cytometry ZW15_03 Co-Principal Investigator (Co-PI)	Fonds Wetenschappelijk Onderzoek Vlaanderen
2016 - 2020	A platform comprising complementary fluidics pipelines for high-throughput single-cell processing, sequencing and data analysis STK2015-143 Co-Principal Investigator (Co-PI)	Fondation contre le Cancer
2016 - 2020	Live tissue imaging platform with enhanced confocal detection and full non-linear excitation possibilities AKUL/15/37 - G0H1916N Co-Principal Investigator (Co-PI)	Fonds Wetenschappelijk Onderzoek Vlaanderen
2018 - 2019	Tailored AAV delivery systems that boost production of anti-BACE1 nanobodies for effective treatment of Alzheimer’s disease #20170030 Principal investigator	Stichting Alzheimer Onderzoek
2017 - 2019	Hi-Fi: Synapses in action: from high-throughput fluorescence imaging assays of synaptic plasticity to drug discovery HBC.2019.2926 Co-Principal Investigator (Co-PI)	Vlaanderen Agentschap Innoveren & Ondernemen
2015/01 - 2018/12	Single cell heterogeneity of hippocampal astrocytes: investigating molecular diversity and its correlation to functional specialization G066715N Principal investigator	Fonds Wetenschappelijk Onderzoek Vlaanderen
2016/12 - 2018/05	Alzheimer’s disease and AAV9: Use of a virus-based delivery system for vectored immunoprophylaxis in dementia. 713755 Principal investigator	European Research Council
2017 - 2018	Role/s of CYFIP1 in brain development and implications in neurodevelopmental disorders G088415N Principal investigator	Fonds Wetenschappelijk Onderzoek Vlaanderen
2014 - 2018	Microfluidics for single-cell 'omics G0F1914N Co-Principal Investigator (Co-PI)	Fonds Wetenschappelijk Onderzoek Vlaanderen
2016/01 - 2017/12	Spatial transcriptomic analysis of amyloid-induced astrogliosis in early Alzheimer’s disease S#16025 Principal investigator	Stichting voor Alzheimer Onderzoek
2015/01 - 2016/12	New roles for old actors: immune molecule function in astrocytes 1527315N Principal investigator	Fonds Wetenschappelijk Onderzoek Vlaanderen
2012/01 - 2016/12	Molecular Studies of Astrocyte Function in Health and Disease 281961 Principal investigator	European Research Council
2016 - 2016	Use of an AAV9 based system for vectored immunoprophylaxis (VIP) targeting SOD1 in motor neuron disease. SOD-VIP 1513616N Principal investigator	Fondation Thierry Latran Fonds Wetenschappelijk Onderzoek Vlaanderen
2016 - 2016	Validation of single cell transcriptomic data using multiplexed in situ hybridization Internal Funding Principal investigator	Vlaams Instituut voor Biotechnologie
2015 - 2016	Delivery of recombinant AAV9 encoding an antibody against BACE1 to treat Alzheimer's disease P#14006 Principal investigator	Stichting Alzheimer Onderzoek
2014 - 2015	Dual-color implantable mini-fluorescence microscope for imaging brain function in behaving rodents Internal Funding Principal investigator	Vlaams Instituut voor Biotechnologie
2014/01 - 2014/01	Plastic circuits: in vivo imaging of astrocyte-neuron interactions in the mouse brain 1523014N	Fonds Wetenschappelijk Onderzoek Vlaanderen

Outputs

Publications

Book chapter	Francisco Pestana; T. Grant Belgard; Thierry Voet; Matthew G. Holt. "Studying the Role of Astrocytes at Synapses Using Single-Cell Transcriptomics". 2024. 10.1007/978-1-0716-3742-5_9 Grønborg, M.; Holt, M.; Richter, F.; Takamori, S.; Riedel, D.; Jahn, R.; Urlaub, H.. "Proteome analysis of synaptic vesicles". In Proteomics of the nervous system. Wiley, 2008. Published
Conference paper	Castorph, S.; Arleth, L.; Sztucki, M.; Vainio, U.; Ghosh, S.K.; Holt, M.; Jahn, R.; Salditt, T.. "Synaptic vesicles studied by SAXS: Derivation and validation of a model form factor". 2010. 10.1088/1742-6596/247/1/012015
Conference poster	Pereira, Maria João; Ayana, Rajagopal; Holt, Matthew; Arckens, Lutgarde. "Manipulating astrocytic activity as a gateway to modulate adult neuroplasticity". Paper presented in XVI European Meeting on Glial Cells in Health and Disease, 2023. Pereira, Maria João; Holt, Matthew; Arckens, Lutgarde. "Star Power - Elucidating the mechanisms by which astrocytes promote neuroplasticity in adulthood". Paper presented in Leuven Brain Institute (LBI) Scientific meeting, 2022. Pereira, Maria João; Holt, Matthew; Arckens, Lutgarde. "Star Power - Elucidating the mechanisms by which astrocytes promote adult brain plasticity". Paper presented in VI Symposium of the Portuguese Glia Network, 2022. Machado, João Luís; Viana, João Filipe; Veiga, Alexandra; Teixeira, Eduardo; Abreu, Daniela Sofia; Wahis, Jerôme; Liu, Chen; Holt, Matthew; Oliveira, João Filipe. "Morphological analysis of astrocytes in different preparations". Paper presented in FENS Forum 2022 July 9 ¿ 13 in Paris, 2022.
Journal article	Jérôme Wahis; Cansu Akkaya; Andre M. Kirunda; Aline Mak; Karen Zeise; Jens Verhaert; Hayk Gasparyan; Sargis Hovhannisyan; Matthew G. Holt. "The astrocyte a1A-adrenoreceptor is a key component of the neuromodulatory system in mouse visual cortex". Glia (2024): https://doi.org/10.1002/glia.24591. 10.1002/glia.24591 Araks Martirosyan; Rizwan Ansari; Francisco Pestana; Katja Hebestreit; Hayk Gasparyan; Razmik Aleksanyan; Silvia Hnatova; et al. "Unravelling cell type-specific responses to Parkinson’s Disease at single cell resolution". Molecular Neurodegeneration (2024): http://dx.doi.org/10.1186/s13024-023-00699-0. 10.1186/s13024-023-00699-0 Maria João Pereira; Rajagopal Ayana; Matthew G. Holt; Lutgarde Arckens. "Chemogenetic manipulation of astrocyte activity at the synapse— a gateway to manage brain disease". Frontiers in Cell and Developmental Biology (2023): http://dx.doi.org/10.3389/fcell.2023.1193130. 10.3389/fcell.2023.1193130 João Filipe Viana; João Luís Machado; Daniela Sofia Abreu; Alexandra Veiga; Sara Barsanti; Gabriela Tavares; Manuella Martins; et al. "Astrocyte structural heterogeneity in the mouse hippocampus". Glia (2023): http://dx.doi.org/10.1002/glia.24362. 10.1002/glia.24362 Pierre Lemaitre; Samar HK Tareen; Emanuela Pasciuto; Loriana Mascali; Araks Martirosyan; Zsuzsanna Callaerts-Vegh; Suresh Poovathingal; et al. "Molecular and cognitive signatures of ageing partially restored through synthetic delivery of IL2 to the brain". EMBO Molecular Medicine (2023): https://doi.org/10.15252/emmm.202216805. 10.15252/emmm.202216805 Juan P Bolaños; Matthew G. Holt. "Astrocyte heterogeneity and interactions with local neural circuits". Essays in Biochemistry (2023): https://doi.org/10.1042/EBC20220136. 10.1042/EBC20220136 Matthew Holt; Disha Shah; Willy Gsell; Jérôme Wahis; Emma S. Luckett; Tarik Jamoulle; Ben Vermaercke; et al. "Astrocyte calcium dysfunction causes early network hyperactivity in Alzheimer’s disease". Cell Reports 40 8 (2022): 111280-111280. http://dx.doi.org/10.1016/j.celrep.2022.111280. 10.1016/j.celrep.2022.111280 Karl Frontzek; Marco Bardelli; Assunta Senatore; Anna Henzi; Regina R. Reimann; Seden Bedir; Marika Marino; et al. "A conformational switch controlling the toxicity of the prion protein". Nature Structural and Molecular Biology 29 8 (2022): 831-840. http://dx.doi.org/10.1038/s41594-022-00814-7. 10.1038/s41594-022-00814-7 Matthew Holt; Lidia Yshii; Emanuela Pasciuto; Pascal Bielefeld; Loriana Mascali; Pierre Lemaitre; Marika Marino; et al. "Astrocyte-targeted gene delivery of interleukin 2 specifically increases brain-resident regulatory T cell numbers and protects against pathological neuroinflammation". Nature Immunology 23 6 (2022): 878-891. http://dx.doi.org/10.1038/s41590-022-01208-z. 10.1038/s41590-022-01208-z Matthew Holt; Ahmed Abdelfattah; Srinivasa Rao Allu; Robert E. Campbell; Xiaojun Cheng; Tomáš Cižmár; Irene Costantini; et al. "Neurophotonic Tools for Microscopic Measurements and Manipulation: Status Report". Neurophotonics 9 S1 (2022): http://dx.doi.org/10.1117/1.nph.9.s1.013001. 10.1117/1.nph.9.s1.013001 Matthew Holt; Marika Marino; Matthew G. Holt. "AAV Vector-Mediated Antibody Delivery (A-MAD) in the Central Nervous System". Frontiers in Neurology 13 (2022): http://dx.doi.org/10.3389/fneur.2022.870799. 10.3389/fneur.2022.870799 Marika Marino; Lujia Zhou; Melvin Y Rincon; Zsuzsanna Callaerts-Vegh; Jens Verhaert; Jérôme Wahis; Eline Creemers; et al. "AAV-mediated delivery of an anti-BACE1 VHH alleviates pathology in an Alzheimer's disease model". EMBO Molecular Medicine 14 4 (2022): http://dx.doi.org/10.15252/emmm.201809824. 10.15252/emmm.201809824 Shinmyo Y; Saito K; Hamabe-Horiike T; Kameya N; Ando A; Kawasaki K; Duong TAD; et al. "Localized astrogenesis regulates gyrification of the cerebral cortex.". Science advances (2022): http://europepmc.org/abstract/med/35275722. 10.1126/sciadv.abi5209 Gong JY; Holt MG; Hoet PHM; Ghosh M. "Neurotoxicity of four frequently used nanoparticles: a systematic review to reveal the missing data.". Archives of toxicology (2022): http://europepmc.org/abstract/med/35278105. 10.1007/s00204-022-03233-1 Matthew Holt; Jérôme Wahis; Maroussia Hennes; Lutgarde Arckens; Matthew G Holt. "Star power: the emerging role of astrocytes as neuronal partners during cortical plasticity". Current Opinion in Neurobiology 67 (2021): 174-182. http://dx.doi.org/10.1016/j.conb.2020.12.001. 10.1016/j.conb.2020.12.001 Escartin C; Galea E; Lakatos A; O'Callaghan JP; Petzold GC; Serrano-Pozo A; Steinhäuser C; et al. "Reactive astrocyte nomenclature, definitions, and future directions.". Nature neuroscience (2021): http://europepmc.org/abstract/med/33589835. 10.1038/s41593-020-00783-4 Wahis J; Holt MG. "Astrocytes, Noradrenaline, a1-Adrenoreceptors, and Neuromodulation: Evidence and Unanswered Questions.". Frontiers in cellular neuroscience (2021): http://europepmc.org/abstract/med/33716677. 10.3389/fncel.2021.645691 Eykens C; Rossaert E; Duqué S; Rué L; Bento-Abreu A; Hersmus N; Lenaerts A; et al. "AAV9-mediated gene delivery of MCT1 to oligodendrocytes does not provide a therapeutic benefit in a mouse model of ALS.". Molecular therapy. Methods & clinical development (2021): http://europepmc.org/abstract/med/33614825. 10.1016/j.omtm.2021.01.006 Serneels L; T'Syen D; Perez-Benito L; Theys T; Holt MG; De Strooper B. "Modeling the ß-secretase cleavage site and humanizing amyloid-beta precursor protein in rat and mouse to study Alzheimer's disease.". Molecular neurodegeneration (2020): http://europepmc.org/abstract/med/33076948. 10.1186/s13024-020-00399-z Holt, Matthew. "Astrocytes shape the plastic response of adult cortical neurons to vision loss.". Glia (2020): https://doi.org/10.1002/glia.23830. 10.1002/glia.23830 Holt, Matthew. "Astrocyte layers in the mammalian cerebral cortex revealed by a single-cell in situ transcriptomic map.". Nature neuroscience (2020): https://doi.org/10.1038/s41593-020-0602-1. 10.1038/s41593-020-0602-1 Francisco Pestana; Gabriela Edwards-Faret; T. Grant Belgard; Araks Martirosyan; Matthew G. Holt. "No Longer Underappreciated: The Emerging Concept of Astrocyte Heterogeneity in Neuroscience". Brain Sciences 10 3 (2020): 168-168. https://doi.org/10.3390/brainsci10030168. 10.3390/brainsci10030168 Holt, Matthew. "Identification of region-specific astrocyte subtypes at single cell resolution.". Nature communications (2020): https://europepmc.org/articles/PMC7058027. 10.1038/s41467-019-14198-8 Holt, Matthew. "Distinct Mechanisms for Visual and Motor-Related Astrocyte Responses in Mouse Visual Cortex.". Current biology : CB (2019): https://doi.org/10.1016/j.cub.2019.07.078. 10.1016/j.cub.2019.07.078 Holt, Matthew. "Production, Purification, and Quality Control for Adeno-associated Virus-based Vectors.". Journal of visualized experiments : JoVE (2019): https://doi.org/10.3791/58960. 10.3791/58960 Holt, Matthew. "Widespread transduction of astrocytes and neurons in the mouse central nervous system after systemic delivery of a self-complementary AAV-PHP.B vector.". Gene therapy (2018): https://doi.org/10.1038/s41434-018-0005-z. 10.1038/s41434-018-0005-z Holt M. "Satnav for cells: Destination membrane fusion.". Cell calcium (2017): http://europepmc.org/abstract/med/29129204. 10.1016/j.ceca.2017.10.001 Zhou L; McInnes J; Wierda K; Holt M; Herrmann AG; Jackson RJ; Wang YC; et al. "Tau association with synaptic vesicles causes presynaptic dysfunction.". Nature communications (2017): http://europepmc.org/abstract/med/28492240. 10.1038/ncomms15295 Batiuk MY; de Vin F; Duqué SI; Li C; Saito T; Saido T; Fiers M; Belgard TG; Holt MG. "An immunoaffinity-based method for isolating ultrapure adult astrocytes based on ATP1B2 targeting by the ACSA-2 antibody.". The Journal of biological chemistry (2017): http://europepmc.org/abstract/med/28373281. 10.1074/jbc.M116.765313 Koch M; Nicolas M; Zschaetzsch M; de Geest N; Claeys A; Yan J; Morgan MJ; et al. "A Fat-Facets-Dscam1-JNK Pathway Enhances Axonal Growth in Development and after Injury.". Frontiers in cellular neuroscience (2017): http://europepmc.org/abstract/med/29472843. 10.3389/fncel.2017.00416 Segura I; Lange C; Knevels E; Moskalyuk A; Pulizzi R; Eelen G; Chaze T; et al. "The Oxygen Sensor PHD2 Controls Dendritic Spines and Synapses via Modification of Filamin A.". Cell reports (2016): http://europepmc.org/abstract/med/26972007. 10.1016/j.celrep.2016.02.047 Muhammad K; Reddy-Alla S; Driller JH; Schreiner D; Rey U; Böhme MA; Hollmann C; et al. "Presynaptic spinophilin tunes neurexin signalling to control active zone architecture and function.". Nature communications (2015): http://europepmc.org/abstract/med/26471740. 10.1038/ncomms9362 Kremer A; Lippens S; Bartunkova S; Asselbergh B; Blanpain C; Fendrych M; Goossens A; et al. "Developing 3D SEM in a broad biological context.". Journal of microscopy (2015): http://europepmc.org/abstract/med/25623622. 10.1111/jmi.12211 Depner H; Lützkendorf J; Babkir HA; Sigrist SJ; Holt MG. "Differential centrifugation-based biochemical fractionation of the Drosophila adult CNS.". Nature protocols (2014): http://europepmc.org/abstract/med/25393777. 10.1038/nprot.2014.192 Andlauer TF; Scholz-Kornehl S; Tian R; Kirchner M; Babikir HA; Depner H; Loll B; et al. "Drep-2 is a novel synaptic protein important for learning and memory.". eLife (2014): http://europepmc.org/abstract/med/25392983. 10.7554/eLife.03895 Tudor C; te Riet J; Eich C; Harkes R; Smisdom N; Bouhuijzen Wenger J; Ameloot M; et al. "Syntenin-1 and ezrin proteins link activated leukocyte cell adhesion molecule to the actin cytoskeleton.". The Journal of biological chemistry (2014): http://europepmc.org/abstract/med/24662291. 10.1074/jbc.M113.546754 Boesmans W; Rocha NP; Reis HJ; Holt M; Vanden Berghe P. "The astrocyte marker Aldh1L1 does not reliably label enteric glial cells.". Neuroscience letters (2014): http://europepmc.org/abstract/med/24589880. 10.1016/j.neulet.2014.02.042 Kiessling V; Ahmed S; Domanska MK; Holt MG; Jahn R; Tamm LK. "Rapid fusion of synaptic vesicles with reconstituted target SNARE membranes.". Biophysical journal (2013): http://europepmc.org/abstract/med/23663838. 10.1016/j.bpj.2013.03.038 Ahmed S; Holt M; Riedel D; Jahn R. "Small-scale isolation of synaptic vesicles from mammalian brain.". Nature protocols (2013): http://europepmc.org/abstract/med/23619891. 10.1038/nprot.2013.053 Park Y; Hernandez JM; van den Bogaart G; Ahmed S; Holt M; Riedel D; Jahn R. "Controlling synaptotagmin activity by electrostatic screening.". Nature structural & molecular biology (2012): http://europepmc.org/abstract/med/22940675. 10.1038/nsmb.2375 Matta S; Van Kolen K; da Cunha R; van den Bogaart G; Mandemakers W; Miskiewicz K; De Bock PJ; et al. "LRRK2 controls an EndoA phosphorylation cycle in synaptic endocytosis.". Neuron (2012): http://europepmc.org/abstract/med/22998870. 10.1016/j.neuron.2012.08.022 Owald D; Khorramshahi O; Gupta VK; Banovic D; Depner H; Fouquet W; Wichmann C; et al. "Cooperation of Syd-1 with Neurexin synchronizes pre- with postsynaptic assembly.". Nature neuroscience (2012): http://europepmc.org/abstract/med/22864612. 10.1038/nn.3183 Ghosh SK; Castorph S; Konovalov O; Salditt T; Jahn R; Holt M. "Measuring Ca2+-induced structural changes in lipid monolayers: implications for synaptic vesicle exocytosis.". Biophysical journal (2012): http://europepmc.org/abstract/med/22455922. 10.1016/j.bpj.2012.01.006 Koch M; Holt M. "Coupling exo- and endocytosis: an essential role for PIP2 at the synapse.". Biochimica et biophysica acta (2012): http://europepmc.org/abstract/med/22387937. 10.1016/j.bbalip.2012.02.008 Castorph S; Schwarz Henriques S; Holt M; Riedel D; Jahn R; Salditt T. "Synaptic vesicles studied by dynamic light scattering.". The European physical journal. E, Soft matter (2011): http://europepmc.org/abstract/med/21706281. 10.1140/epje/i2011-11063-2 van den Bogaart G; Thutupalli S; Risselada JH; Meyenberg K; Holt M; Riedel D; Diederichsen U; et al. "Synaptotagmin-1 may be a distance regulator acting upstream of SNARE nucleation.". Nature structural & molecular biology (2011): http://europepmc.org/abstract/med/21642968. 10.1038/nsmb.2061 Groemer TW; Thiel CS; Holt M; Riedel D; Hua Y; Hüve J; Wilhelm BG; Klingauf J. "Amyloid precursor protein is trafficked and secreted via synaptic vesicles.". PloS one (2011): http://europepmc.org/abstract/med/21556148. 10.1371/journal.pone.0018754 Matthew Holt; S K Ghosh; S Castorph; O Konovalov; R Jahn; M Holt; T Salditt. "In vitro study of interaction of synaptic vesicles with lipid membranes". New Journal of Physics 12 10 (2010): 105004-105004. http://dx.doi.org/10.1088/1367-2630/12/10/105004. 10.1088/1367-2630/12/10/105004 Castorph S; Riedel D; Arleth L; Sztucki M; Jahn R; Holt M; Salditt T. "Structure parameters of synaptic vesicles quantified by small-angle x-ray scattering.". Biophysical journal (2010): http://europepmc.org/abstract/med/20371319. 10.1016/j.bpj.2009.12.4278 van den Bogaart G; Holt MG; Bunt G; Riedel D; Wouters FS; Jahn R. "One SNARE complex is sufficient for membrane fusion.". Nature structural & molecular biology (2010): http://europepmc.org/abstract/med/20139985. 10.1038/nsmb.1748 Rummel A; Häfner K; Mahrhold S; Darashchonak N; Holt M; Jahn R; Beermann S; et al. "Botulinum neurotoxins C, E and F bind gangliosides via a conserved binding site prior to stimulation-dependent uptake with botulinum neurotoxin F utilising the three isoforms of SV2 as second receptor.". Journal of neurochemistry (2009): http://europepmc.org/abstract/med/19650874. 10.1111/j.1471-4159.2009.06298.x Hosoi N; Holt M; Sakaba T. "Calcium dependence of exo- and endocytotic coupling at a glutamatergic synapse.". Neuron (2009): http://europepmc.org/abstract/med/19640480. 10.1016/j.neuron.2009.06.010 Holt M; Riedel D; Stein A; Schuette C; Jahn R. "Synaptic vesicles are constitutively active fusion machines that function independently of Ca2+.". Current biology : CB (2008): http://europepmc.org/abstract/med/18485705. 10.1016/j.cub.2008.04.069 Takamori S; Holt M; Stenius K; Lemke EA; Grønborg M; Riedel D; Urlaub H; et al. "Molecular anatomy of a trafficking organelle.". Cell (2006): http://europepmc.org/abstract/med/17110340. 10.1016/j.cell.2006.10.030 Holt M; Varoqueaux F; Wiederhold K; Takamori S; Urlaub H; Fasshauer D; Jahn R. "Identification of SNAP-47, a novel Qbc-SNARE with ubiquitous expression.". The Journal of biological chemistry (2006): http://europepmc.org/abstract/med/16621800. 10.1074/jbc.m513838200 Holt M; Jahn R. "Neuroscience. Synaptic vesicles in the fast lane.". Science (New York, N.Y.) (2004): http://europepmc.org/abstract/med/15044796. 10.1126/science.1096747 Holt M; Cooke A; Neef A; Lagnado L. "High mobility of vesicles supports continuous exocytosis at a ribbon synapse.". Current biology : CB (2004): http://europepmc.org/abstract/med/14761649. 10.1016/j.cub.2003.12.053 Holt, M.; Cooke, A.; Neef, A.; Lagnado, L.. "High Mobility of Vesicles Supports Continuous Exocytosis at a Ribbon Synapse". Current Biology 14 3 (2004): 173-183. http://www.scopus.com/inward/record.url?eid=2-s2.0-1242310783&partnerID=MN8TOARS. 10.1016/S0960-9822(03)00973-4 Holt M; Cooke A; Wu MM; Lagnado L. "Bulk membrane retrieval in the synaptic terminal of retinal bipolar cells.". The Journal of neuroscience : the official journal of the Society for Neuroscience (2003): http://europepmc.org/abstract/med/12598621. 10.1523/jneurosci.23-04-01329.2003 O'Brien JA; Holt M; Whiteside G; Lummis SC; Hastings MH. "Modifications to the hand-held Gene Gun: improvements for in vitro biolistic transfection of organotypic neuronal tissue.". Journal of neuroscience methods (2001): http://europepmc.org/abstract/med/11640958. 10.1016/s0165-0270(01)00457-5
Preprint	Jérôme Wahis; Cansu Akkaya; Andre M. Kirunda; Aline Mak; Karen Zeise; Jens Verhaert; Hayk Gasparyan; Sargis Hovhannisyan; Matthew G. Holt. "The astrocyte a1-adrenoreceptor is a key component of the neuromodulatory system in mouse visual cortex". 2023. https://doi.org/10.1101/2023.05.02.538934. 10.1101/2023.05.02.538934 Lidia YSHII; Loriana Mascali; Lubna Kouser; Pierre Lemaitre; Marika Marino; James Dooley; Oliver Burton; et al. "The AppNL-G-F mouse model of Alzheimer’s disease is refractory to regulatory T cell treatment". 2022. http://dx.doi.org/10.1101/2022.03.11.483903. 10.1101/2022.03.11.483903 "AAV mediated delivery of a novel anti-BACE1 VHH reduces Abeta in an Alzheimer’s disease mouse model". 2019. http://dx.doi.org/10.1101/698506. 10.1101/698506
Thesis / Dissertation	Machado, Maria Eugénia Freire Torres Alves. "Vectored immunoprophylaxis to produce anti-BACE1 nanobodies for treatment of Alzheimer's disease". Master, 2019. http://hdl.handle.net/10451/40536.

Other

Other output

Molecular and cognitive signatures of ageing partially restored through synthetic delivery of IL2 to the brain. 2022. Lemaitre, P.; Tareen, S.; Pasciuto, E.; Mascali, L.; Martirosyan, A.; Callaerts-Vegh, Z.; Dooley, J.; et al. http://www.scopus.com/inward/record.url?eid=2-s2.0-85128987197&partnerID=MN8TOARS.
10.1101/2022.03.01.482519
Flexible, fast and selective genetic manipulation of the vertebrate CNS with misPiggy. 2018. Slezak, M.; de Vin, F.; Shinmyo, Y.; Batiuk, M.Y.; Rincon, M.Y.; Pando, C.M.; Urschitz, J.; et al. http://www.scopus.com/inward/record.url?eid=2-s2.0-85095644876&partnerID=MN8TOARS.
10.1101/481580
Astrocytes integrate local sensory and brain-wide neuromodulatory signals. 2018. Slezak, M.; Kandler, S.; Van Veldhoven, P.P.; Bonin, V.; Holt, M.G.. http://www.scopus.com/inward/record.url?eid=2-s2.0-85095660573&partnerID=MN8TOARS.
10.1101/381434

Activities

Oral presentation

	Presentation title	Event name Host (Event location)
2024/11	Career development talk	University of Amsterdam (Amsterdam, Netherlands)
2024/11	Invited talk	Swammerdam Lecture University of Amsterdam (Amsterdam, Netherlands)
2024/07	Invited talk	Scuola Internazionale Superiore di Studi Avanzati Scuola Internazionale Superiore di Studi Avanzati (Trieste, Italy)
2024/04	Invited talk	University of Padova (Padova, Italy)
2024/03	Invited talk	Gordon Research Conference: Antibody Biology and Engineering (Italy)
2024/02	Invited talk	Institut du Cerveau et de la Moelle épinière (France)
2023/11	Keynote talk: The emerging concept of astrocyte heterogeneity: Consequences for CNS function	Astrocytes and ApoE mini-symposium (Leuven, Belgium)
2023/09	Keynote talk: AAV-mediated delivery of an anti-BACE1 VHH alleviates pathology in an Alzheimer’s disease model	Nanobody Symposium (Paris, France)
2023/08	Astrocyte heterogeneity and interactions with local circuits	International Society for Neurochemistry Satellite Meeting (Portugal)
2023/05	Career development talk (ERC information session)	FENS Regional Meeting (Algarve, Portugal)
2023/05	New tools to study the brain in health and disease	FENS Regional Meeting (Portugal)
2022/11	Molecular Heterogeneity of Astrocytes: Potential Implications for Development and Function	i3S Neuro Day (Porto, Portugal)
2022/10	Keynote Talk: “Molecular Heterogeneity of Astrocytes: Potential Implications for Development and Function”	Portuguese Glia Meeting (Porto, Portugal)
2022/10	Astrocytes: Master regulators of inhibition and plasticity	i3S Group Leaders’ Seminar Series (Porto, Portugal)
2022/09	Invited Talk	ITN AstroTech Workshop (Porto, Portugal)
2022/07	AAV-mediated delivery of an anti-BACE1 VHH alleviates pathology in an Alzheimer’s disease model	Alzheimer’s Association International Conference (United States)
2022/07	The emerging concept of astrocyte heterogeneity: consequences for CNS function	FENS Forum (France)
2022/07	Astrocytes: Master regulators of inhibition and plasticity?	FENS Glia Satellite (Paris, France)
2022/06	Astrocytes: Master regulators of inhibition and plasticity?	Institute of Molecular Medicine (Lisbon, Portugal)
2022/06	Spatial Transcriptomics: mapping gene expression and cell populations in the brain	Dutch Neuroscience Society (Tiel, Netherlands)
2022/05	Experiences of an ERA Chair	Science Europe Workshop on Widening Participation and Spreading
2022/05	Astrocytes: Master regulators of inhibition and plasticity?	Belgian Neuroscience Society (Brussels, Belgium)
2021/06	Mapping transcriptome heterogeneity in the CNS with single cell resolution using highly multiplexed spatial transcriptomics	American Society for Neurochemistry - Advances in Genome Biology and Technology (Florida, United States)
2020/11	Invited talk	Neuroscience Ireland Young Investigators Symposium (Ireland)
2020/10	Invited talk	Neurophotonics Virtual Mini-Symposium
2020/09	Invited talk	University of Maastricht (Maastricht, Netherlands)
2020/09	Invited talk	OpenBox Science Symposium, Virtual Conference
2020/02	Career development talk	Careers in neuroscience symposium University of Luxembourg (Luxembourg)
2020/02	Invited talk	University of Luxembourg (Luxembourg)
2020/01	Invited talk	Resolve Life Sciences (formerly Qiagen) (Germany)
2019/12	Invited talk	Vrije Universiteit Brussels (Belgium)
2019/07	Invited talk	Ludwig Maximilians-Universiteit (Germany)
2019/07	Invited talk	European Glia Conference (Portugal)
2019/06	Invited talk	Lowlands Neuroscience Meeting (Belgium)
2019/03	Invited talk	Gordon Research Conference, Glial Biology: Functional Interactions Among Glia and Neurons (United States)
2019/02	Invited talk	University of Osnabrück (Germany)
2018/09	Invited talk	University of Lausanne (Lausanne, Switzerland)
2017/07	Invited talk	Cajal Neuroscience School (France)
2017/05	Invited talk	Thierry Latran Meeting (Slovenia)
2017/01	Invited talk	University of Amsterdam (Amsterdam, Netherlands)
2016/05	Invited talk	Thierry Latran Meeting (Italy)
2016/05	Invited talk	Janssen Pharma (Belgium)
2016/03	Career development talk (ERC information session)	University of Utrecht (Utrecht, Netherlands)
2016/03	Invited talk	University of Utrecht (Utrecht, Netherlands)
2015/11	Invited talk	University of Maastricht (Maastricht, Netherlands)
2015/04	Invited talk	KU Leuven Biomedical Congress (Belgium)
2015/03	Invited talk	KU Leuven Stem Cell Institute (Belgium)
2014/10	Invited talk	Bogaziçi University (Turkey)
2014/09	Invited talk	VIB Neurogenetics Unit (Belgium)
2014/06	Invited talk	Scripps Research Institute (United States)
2013/05	Invited talk	Neuronus Conference (Poland)
2013/01	Invited talk	University of Lausanne (Lausanne, Switzerland)
2012/11	Invited talk	Université Libre de Bruxelles (Brussels, Belgium)
2012/05	Career development talk (ERC information session)	Leadnet Conference Max Planck Society (Berlin, Germany)
2012/03	Invited talk	International Astrocyte Summer School (Italy)

Supervision

	Thesis Title Role	Degree Subject (Type) Institution / Organization
2024 - Current	Development of Cas9-based tools for astrocyte-specific manipulation Supervisor of Rafaela Seixas	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2023 - Current	Identification of astrocyte-secreted factors involved in synapse formation Supervisor of João Baltar Guimarães	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2023 - Current	Development of TurboID-based systems for secretome analysis Supervisor of Luísa Florido	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2023 - Current	Implementation of TurboID-based systems for surface proteome analysis Supervisor of Carlos Pinto	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2022 - Current	Establishing the molecular basis and function of the astrocyte-neuron interplay at the inhibitory tripartite synapse. Supervisor of Domenico Natale	Associatie KU Leuven, Belgium
2021 - Current	Critical periods and brain plasticity – astrocytes as key modulators of cortical wiring. Supervisor of Maria João Pereira	Associatie KU Leuven, Belgium
2020 - Current	Investigating the protocadherin system in astrocytes: a cross species study on their involvement in formation of brain circuitry. Supervisor of Francisco Pestana	Associatie KU Leuven, Belgium
2019 - Current	Postdoctoral Researcher in Dr. Matthew Holt's lab. Supervisor of Dr. Sarah van Veen	Associatie KU Leuven, Belgium
2017 - Current	Postdoctoral Researcher in Dr. Matthew Holt's lab. Supervisor	Associatie KU Leuven, Belgium
2023/11/06 - 2024/03/01	Generation and in vitro validation of CRISPR/Cas9-based knockout of proteins in astrocytes Supervisor of Julia Oster	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2022 - 2022/09	Optimized AAV systems for vectored immunoprophylaxis as a therapeutic strategy to treat CNS disease. Supervisor of Marika Marino	Associatie KU Leuven, Belgium
2021 - 2022	Investigating cell type heterogeneity in the brain using machine learning techniques. Supervisor of Hayk Gasparyan	Yerevan State University, Armenia
2021 - 2022	Single image dehazing. Supervisor of Sargis Hovhannisyan	Yerevan State University, Armenia
2021 - 2022	Solving the problems of classification and segmentation of brain cells. Supervisor of Razmik Aleksanyan	Yerevan State University, Armenia
2021 - 2022	Postdoctoral Researcher in Dr. Matthew Holt's lab. Supervisor of Dr. Çansu Akkaya	Associatie KU Leuven, Belgium
2017 - 2022	Postdoctoral Researcher in Dr. Matthew Holt's lab. Supervisor of Dr. Araks Martirosyan	Associatie KU Leuven, Belgium
2019 - 2021	Postdoctoral Researcher in Dr. Matthew Holt's lab. Supervisor of Dr. Lidia Yshii	Associatie KU Leuven, Belgium
2020 - 2020/12	Characterizing the missing cellular component in visual cortex plasticity. Co-supervisor of Maroussia Hennes	Associatie KU Leuven, Belgium
2020 - 2020/09	Target specific routing of visual information by the superior colliculus. Co-supervisor of Chen Li	Associatie KU Leuven, Belgium
2020 - 2020	Optimisation of an rAAV-based tool for the treatment of CNS disorders. Supervisor of Jens Verhaert	UC Leuven-Limburg, Belgium
2020 - 2020	Studying neuromodulatory functions of astrocytes using cell-type specific genetic tools. Supervisor of Thomas Alkins	UC Leuven-Limburg, Belgium
2020 - 2020	Solving the problems of classification and segmentation of brain cells. Supervisor of Razmik Aleksanyan	Yerevan State University, Armenia
2018 - 2019	Vectored immunoprophylaxis in the CNS. Supervisor of Eugénia Machado	Universidade de Lisboa, Portugal
2018 - 2019	Astrocytes as mediators in noradrenergic modulation of long-term potentiation in visual cortex. Supervisor of Aline Mak	Vrije Universiteit Amsterdam, Netherlands
2018 - 2019	Postdoctoral Researcher in Dr. Matthew Holt's lab. Supervisor of Dr. Jordan Koeppen	Associatie KU Leuven, Belgium
2017 - 2019	Postdoctoral Researcher in Dr. Matthew Holt's lab. Supervisor of Dr. Melina Figueiredo	Associatie KU Leuven, Belgium
2016 - 2019	Postdoctoral Researcher in Dr. Matthew Holt's lab. Supervisor of Dr. Melvin Rincon	Associatie KU Leuven, Belgium
2018 - 2018/11	Study of astrocyte heterogeneity using single cell transcriptomics. Supervisor of Mykhailo Batiuk	Associatie KU Leuven, Belgium
2017 - 2018	Development of new systems for enhanced gene therapy in the central nervous system. Supervisor of Shelly Fripont	Associatie KU Leuven, Belgium
2017 - 2017	Validation of effective silencing of p53 with a p53 shRNA strategy. Supervisor of Catherine Marneffe	Université de Namur, Belgium
2014 - 2017	Postdoctoral Researcher in Dr. Matthew Holt's lab. Supervisor of Dr. Sandra Duqué	Associatie KU Leuven, Belgium
2016 - 2016	AAV9-based delivery of anti-SOD1 nanobodies into the CNS has no therapeutic benefit in a SOD1G93A mouse model. Supervisor of Ori Roethler	Vrije Universiteit Amsterdam, Netherlands
2012 - 2016	Postdoctoral Researcher in Dr. Matthew Holt's lab. Supervisor of Dr. Michal Slezak	Associatie KU Leuven, Belgium
2015 - 2015	Visualization of Ca2+ responses in neurons and astrocytes. Supervisor of Vicky Leuris	Plantijn Technical School, Belgium
2014 - 2015	The role of inflammation and glial Notch in the control of behaviour in Drosophila and mice. Co-supervisor of Sedef Dalbeyer	Associatie KU Leuven, Belgium
2014 - 2014	Implementation of organotypic slice culture system to study astrocyte function. Supervisor	Vrije Universiteit Amsterdam, Netherlands
2014 - 2014	Implementation of organotypic slice culture system to study astrocyte function. Supervisor of Ana Sobornova	Vrije Universiteit Amsterdam, Netherlands
2014 - 2014	Expression and purification of recombinant proteins in mammalian tissue culture cells. Supervisor of Tom Mertens	Plantijn Technical School, Belgium
2013 - 2014	A novel tool for astrocyte specific expression of genetic manipulations. Supervisor of Arno Vandebroek	Associatie KU Leuven, Belgium
2013 - 2014	TU tagging to measure gene expression. Supervisor of Ilayda Hasakiogullari	Universiteit Hasselt, Belgium
2013 - 2013	Co-purification of astrocyte process with synaptosomes. Supervisor of Liese Vandevyvere	Plantijn Technical School, Belgium

Event organisation

	Event name Type of event (Role)	Institution / Organization
2023/06/30 - 2023/06/30	Neural Cell Biology (NCBio) Stakeholder Hub Kick-Off Meeting (2023/06/30 - 2023/06/30) Meeting (Member of the Organising Committee)	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2022/10/18 - 2022/11/18	VI Portuguese Glia Meeting (2022/10/18 - 2022/10/18) Meeting (Co-organisor)	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2022/11/04 - 2022/11/04	Meeting of the i3S Program of Neurobiology and Neurologic Diseases (2022/11/04 - 2022/11/04) Meeting (Co-organisor)	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2022 - 2022	Symposium in Single Cell Sequencing and Spatial Transcriptomics, Dutch Neuroscience Meeting (2022 - 2022) Symposium (Co-organisor)
2017/05 - 2017/05	VIB Training Course on Gene Regulation and Tools for Transcriptional Profiling (2017/05 - 2017/05) Workshop (Co-organisor)	Vlaams Instituut voor Biotechnologie, Belgium
2016/09 - 2016/09	VIB Training Course on Single Cell Analysis (2016/09 - 2016/09) Workshop (Co-organisor)	Vlaams Instituut voor Biotechnologie, Belgium
2016/09 - 2016/09	VIB Conference on Single Cell Analysis (Brain Mosaic) (2016/09 - 2016/09) Conference (Co-organisor)	Vlaams Instituut voor Biotechnologie, Belgium
2012/09 - 2012/09	VIB Training Course on ‘Omics Technologies (2012/09 - 2012/09) Workshop (Other)	Vlaams Instituut voor Biotechnologie, Belgium

Event participation

	Activity description Type of event	Event name Institution / Organization
2017/07 - 2017/07	Instructor at the FENS Summer School “Advanced Neuroscience Techniques” Workshop	Federation of European Neuroscience Societies, Belgium
2015/09 - 2015/09	Speaker at the Annalis Ultracentrifuge Training Symposium Symposium
2013/04 - 2013/04	Speaker at the Brain Train Integrative Neuroscience School on Brain Function and Disease Conference

Jury of academic degree

	Topic Role	Candidate name (Type of degree) Institution / Organization
2022/12	Systematic profiling of microRNA-132 functional targetome and its therapeutic potential in Alzheimer's disease. (Thesis) Arguer	Hannah Walgrave (PhD)
2022/11	Modelling amyotrophic lateral sclerosis: Human iPSC-derived astrocytes and neuromuscular junctions in microfluidic devices. (Thesis) Arguer	Katarina Dittlau (PhD)
2022/11	Decoding gene regulation in the fly brain. (Thesis) Arguer	Jasper Janssens (PhD)
2022/09	Radial astrocytes in the developing retinotectal system promote escape behavior through the detection and modulation of neuronal activity. (Thesis) Arguer	Nick Benfey (PhD)
2021/12	Unraveling the role of PREPL, a serine hydrolase deleted in a Prader-Willi-like syndrome. (Thesis) Arguer	Karen Rosier (PhD)
2021/08	Stress-dependent mental disorders: a molecular and structural basis. (Thesis) Arguer	Dorien Vandael (PhD)
2021/07	Generation of excitatory and inhibitory neurons from common progenitors via Notch signaling in the cerebellum. (Thesis) Arguer	Tingting Zhang (PhD)
2021/03	Nanobodies to cross the blood-brain barrier. (Thesis) Arguer	Yessica Wouters (PhD)
2021/03	The role of nucleocytoplasmic transport in C9orf72 amyotrophic lateral sclerosis. (Thesis) Arguer	Joni Vanneste (PhD)
2020/10	Mapping the cell-surface proteome underlying synapse type-specific identity. (Thesis) Arguer	Nuno Apóstolo (PhD)
2020/01	The role of TNFAIP3/A20 in neurolupus. (Thesis) Arguer	Carmen Daems (PhD)
2019/01	Exploration and modulation of oligodendrocyte dysfunction in ALS. (Thesis) Arguer	Caroline Eykens (PhD)
2019	Molecular mechanisms of synaptic vesicle rejuvenation: Skywalker and epilepsy. (Thesis) Arguer	Ina Maes (PhD)
2018/11	Hsp90 mediates membrane deformation and exosome release. (Thesis) Arguer	Yu Chun Wang (PhD)
2018/10	BACE2 distribution in the brain and identification of its substrates. (Thesis) Arguer	Iryna Voytyuk (PhD)
2018/02	The synapse to nucleus signalling is impaired in Fragile X syndrome. (Thesis) Arguer	Laura D’Andrea (PhD)
2017/09	Investigation of a genetic and environmental modifier of sporadic Alzheimer’s disease. (Thesis) Arguer	Isabel Salas (PhD)
2016/10	Inhibition of histone deacetylase 6 (HDAC6) protects against vincristine induced peripheral neuropathies and inhibits tumor progression in vivo. (Thesis) Arguer	Lawrence Van Helleputte (PhD)
2016/09	Modulation of tau pathology propagation. (Thesis) Arguer	Sara Calafate (PhD)
2016/01	Exploring the mechanism and therapeutic potential of the ephrin system in ALS. (Thesis) Arguer	Lies Schoonaert (PhD)
2016/01	Dynamic properties of the alkaline vesicle population at hippocampal synapses. (Thesis) Arguer	Mareike Röther (PhD)

Ad Hoc journal article review

	Journal title (ISSN)	Publisher
2023 - Current	Member of Editorial Board of Brain Organoid and Systems Neuroscience	Elsevier B.V.
2022/10/07 - Current	Member of Editorial Board of GLIA (1098-1136)	Wiley Periodicals, LLC.
2021 - Current	Frontiers in Cell and Developmental Biology (2296-634X)	Frontiers
2019 - Current	Frontiers in Cellular Neuroscience (1662-5102)	Frontiers
2019 - Current	Editorial board member of Brain Sciences (2076-3425 )	MDPI
2019 - Current	Editorial board member of Frontiers in Cellular Neuroscience (1662-5102)	Frontiers
2012 - Current	Acta Neuropathologica Communications (2051-5960)	BMC
2012 - Current	Brain Behavior and Immunity (1090-2139)	Elsevier B.V.
2012 - Current	Brain Structure and Function (1863-2661)	Springer
2012 - Current	Biophysical Journal (1542-0086)	Cell Press
2012 - Current	Cell (1097-4172)	Cell Press
2012 - Current	Cell Calcium (0143-4160)	Elsevier B.V.
2012 - Current	Cell Reports (2211-1247)	Cell Press
2012 - Current	Current Opinion in Neurobiology (1873-6882)	Elsevier B.V.
2012 - Current	Developmental Cell (1878-1551)	Cell Press
2012 - Current	eLife (2050-084X)	eLife Sciences Publications, Ltd
2012 - Current	EMBO Journal (1460-2075)	EMBO Press
2012 - Current	EMBO Molecular Medicine (1757-4684)	EMBO Press
2012 - Current	European Journal of Neuroscience (1460-9568)	Federation of European Neuroscience Societies and John Wiley & Sons Ltd
2012 - Current	FEBS Journal (1742-4658)	FEBS Press
2012 - Current	Glia (1098-1136)	Wiley Periodicals, LLC.
2012 - Current	iScience (2589-0042)	Cell Press
2012 - Current	Journal of Cell Biology (0021-9525)	Rockefeller University Press
2012 - Current	Journal of Cellular Biochemistry (1097-4644)	Wiley Periodicals, LLC.
2012 - Current	Journal of Cellular Physiology (1097-4644)	Wiley Periodicals, LLC.
2012 - Current	Journal of Neuroscience (1529-2401)	Society for Neuroscience
2012 - Current	Journal of Neuroscience Research (1097-4547)	Wiley Periodicals, LLC.
2012 - Current	JoVE (1940-087X)	MyJove Corp.
2012 - Current	Molecular Psychiatry (1476-5578)	Nature Portofolio
2012 - Current	Molecular Therapy (Nucleic Acids) (2162-2531)	Cell Press
2012 - Current	Nature (1476-4687)	Nature Portfolio, Springer Nature
2012 - Current	Nature Communications (2041-1723)	Nature Portofolio
2012 - Current	Nature Neuroscience (1546-1726)	Nature Portofolio
2012 - Current	Neurobiology of Disease (1095-953X)	Elsevier B.V.
2012 - Current	Neuroscience (1873-7544)	Elsevier B.V.
2012 - Current	Plos One (1932-6203)	PLOS
2012 - Current	Plos Pathogens (1553-7374 )	PLOS
2012 - Current	PNAS (1091-6490)	National Academy of Sciences
2012 - Current	Science (0036-8075)	AAAS
2012 - Current	Scientific Reports (2045-2322)	Nature Portofolio
2012 - Current	Theranostics (1838-7640)	Ivyspring International Publisher
2012 - Current	Trends in Cell Biology (1879-3088)	Cell Press
2022 - 2022	Frontiers in Cell and Developmental Biology - Editor of Astrogliogenesis special topic (2296-634X)	Frontiers

Association member

	Society Organization name	Role
2012 - Current	Elected Member of the “Sapienza Academic Network” based at Sapienza University, Rome.

Committee member

	Activity description Role	Institution / Organization
2018 - Current	Member of the oversight panel for gene therapy trials run by the Dementia Research Institute, UK. Member	UK Dementia Research Institute, United Kingdom
2016 - Current	Member of the management committees for the KU Leuven and VIB FACS facilities. Member	Associatie KU Leuven, Belgium
2012 - Current	Member of the evaluation panel for the "Synapses Under Stress" Research Unit. Member	Deutsche Forschungsgemeinschaft, Germany
2012 - Current	Member of the “Talent Stars” selection panel for junior PIs. Member	Università degli Studi di Padova, Italy
2002 - Current	Member of the "Astrocyte Heterogeneity" evaluation panel Member	Deutsche Forschungsgemeinschaft, Germany
2015 - 2016	Member of the VIB Group Leader’s Management Committee (with responsibility for selection of students admitted to the VIB Ph.D. program) Member	VIB - KU Leuven Center for Brain & Disease Research, Belgium

Consulting

	Activity description	Institution / Organization
2012 - Current	Ad hoc review on glia	Agence nationale de la recherche, France
2012 - Current	Ad hoc review on glia	Alzheimer Nederland, Netherlands
2012 - Current	Ad hoc review on glia	Association Européenne contre les Leucodystrophies, France
2012 - Current	Ad hoc review on glia	Biotechnology and Biological Sciences Research Council, United Kingdom
2012 - Current	Ad hoc review on glia	UK Dementia Research Institute, United Kingdom
2012 - Current	Ad hoc review on glia	Wellcome Trust, United Kingdom
2012 - Current	Ad hoc review on glia	Düsseldorf University Internal Funds, Germany
2012 - Current	Ad hoc review on glia (Consolidator Panel and Synergy Panel)	European Research Council, Belgium
2012 - Current	Ad hoc review on glia	European Science Foundation, France
2012 - Current	Ad hoc review on glia	Fédération pour la recherche sur le cerveau, France
2012 - Current	Ad hoc review on glia	Fondation pour la Recherche Médicale, France
2012 - Current	Ad hoc review on glia	Fondazione Cariplo, Italy
2012 - Current	Ad hoc review on glia	Israeli Science Foundation, Israel
2012 - Current	Ad hoc review on glia	Medical Research Council UK, United Kingdom
2012 - Current	Ad hoc review on glia	Nederlandse Organisatie voor Wetenschappelijk Onderzoek, Netherlands
2012 - Current	Ad hoc review on glia	Schweizerischer Nationalfonds zur Förderung der wissenschaftlichen Forschung, Switzerland
2012 - Current	Ad hoc review on glia	Fondation Thierry Latran, France

Course / Discipline taught

	Academic session	Degree Subject (Type)	Institution / Organization
2024 - Current	Faculty Member of the International Astrocyte School, Bertinoro, Italy
2024 - Current	Synapse Assembly, Structure, and Function PhD course	(Doutoramento)	Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2023 - Current	Neuroscience Ph.D. lectures on astrocyte biology	(Doutoramento)	Universidade do Porto, Portugal
2022 - Current	Molecular and Cellular Biology Ph.D. lectures on glial biology	(Doutoramento)	Universidade do Porto, Portugal
2019 - Current	Topics in Neurodegeneration	(Master)	Associatie KU Leuven, Belgium
2012 - Current	Advanced Biology of the Cell/Neuron	(Master)	Associatie KU Leuven, Belgium
2012 - Current	Hot Topics in the Biology of the Cell/Neuron	(Master)	Associatie KU Leuven, Belgium
2012 - 2022	Organizer of the VIB-CBD institutional lecture series		VIB-KU Leuven Center for Brain & Disease Research, Belgium

Evaluation committee

	Activity description Role	Institution / Organization	Funding entity
2023 - 2024	Member of the ATIP-Avenir Evaluation Board (CNRS-Inserm) Member	Centre National de la Recherche Scientifique, France	Centre National de la Recherche Scientifique
2023 - 2023	Member of the ERA-NET NEURON initiative pre-selection panel for ‘Transnational Research Projects on Mechanisms of Resilience and Vulnerability to Environmental Challenges in Mental Health’ run with DFG Member	Deutsche Forschungsgemeinschaft, Germany	Deutsche Forschungsgemeinschaft
2023 - 2023	Member of the Collège de France Scientific Evaluation board (Inserm) Member	Collège de France, France
2022 - 2022	Elected Member of the Sapienza Academic Network Member	Sapienza Università Editrice, Italy
2021 - 2021	Member of the panel for the “Synapses Under Stress” Research Unit Member	Deutsche Forschungsgemeinschaft, Germany	Deutsche Forschungsgemeinschaft
2021 - 2021	Member of the “Talent Stars” selection panel for junior PIs. Member	Università degli Studi di Padova, Italy
2017 - 2017	Member of the “Astrocyte Heterogeneity” panel Member	Deutsche Forschungsgemeinschaft, Germany	Deutsche Forschungsgemeinschaft

Mentoring / Tutoring

	Topic	Student name
2023/09 - Current	Identification of astrocyte cell surface proteins involved in synapse formation	Dr. Maria Joana Pinto (post-doc)
2022 - 2022	Analysis of cell differentiation pathways using RNA Velocity analysis.	Arabo Apresyan (Institute of Molecular Biology NAS RA, Armenia)
2020 - 2020	Effect of Noradrenergic Signalling on Astrocytes and Synaptic Plasticity.	Leon Kremers (Tübingen, Germany]
2019 - 2019	Development of genetic strategies for astrocyte-specific alpha1a-adrenoceptor silencing.	Andre Kirunda (Northeastern University, Boston, USA)
2019 - 2019	Vectored immunoprophylaxis in the CNS.	Laura Manjarres Madrid (CES University, Colombia)
2018 - 2019	Tracing astrocyte development.	Carola Kusserow (University of Freiburg, Germany)
2018 - 2018	Development of genetic strategies for astrocyte-specific alpha1a-adrenoceptor silencing.	Karen Zeise (Northeastern University, Boston, USA)
2017 - 2017	Development of novel in utero electroporation systems.	Carmen Menacho Pando (Universitat Autònoma de Barcelona, Spain)
2017 - 2017	Development of transposon-based genetic tools.	Ana Toledano (Universidad Autonoma de Madrid, Spain)
2017 - 2017	Development of TRAP-based methodologies to study local translation in astrocytes.2017	Sarah Neshat (Northeastern University, Boston, USA)
2016 - 2016	Development of transposon-based lineage tracing tools.	Busra Kirpat (Bilkent University, Turkey)

Distinctions

Award

2022	ERA Chair in Neural Cell Biology EU Framework Programme for Research and Innovation Spreading Excellence and Widening Participation, Belgium
2015	ERC Proof-of-Concept Grant European Research Council, Belgium
2011	ERC Starting Grant European Research Council, Belgium
2010	Fellowship from the Neurocure consortium German Elite University Initiative, Germany
2008	Bombannes-Institut Laue-Langevin Prize for “Scattering Methods Applied to Soft Condensed Matter”
2003	Postdoctoral stipend of the Max Planck Society Max-Planck-Gesellschaft zur Förderung der Wissenschaften, Germany
1998	Ph.D. fellowship from the Medical Research Council Medical Research Council UK, United Kingdom
1998	Glaxo Wellcome Prize for Biochemistry for distinguished performance in undergraduate studies Glaxo Wellcome, United Kingdom
1994	Full faculty scholarships at the University of Liverpool. Awarded to top 1% of students in the faculty. University of Liverpool, United Kingdom

Other distinction

2012	ESRF annual research highlight for work on structure-function analysis of synaptic vesicles ESRF, France