Ana Maria Marques Valentim (9E18-3854-6BA9)

Curriculum Vitae

Ana Maria Marques Valentim

Ana Maria Marques Valentim. Graduated in Biology at the University of Coimbra and completed the PhD in Veterinarian Sciences in 2012/03/01 at Porto University, Instituto de Ciências Biomédicas Abel Salazar (ICBAS). Is a contracted researcher at the Instituto de Biologia Molecular e Celular/ Instituto de Investigação e Inovação em Saúde (i3S), integrated into the Laboratory Animal Science group where she has been developing the research line of anaesthesia refinement in animal models, currently zebrafish, in collaboration with the University of Trás-os-Montes e Alto Douro (UTAD). Recently, she is involved in refining other techniques, such as analgesia and environmental enrichment. Is part of the team of trainers of the Laboratory Animal Science course at i3S (the only course accredited by FELASA in Portugal), and participates in external courses. She is involved in a project to create e-learning modules for the Laboratory Animal Science area for the EU Commission as an expert in the recognition of stress and pain and the biology of zebrafish. Published 42 articles in journals and 3 book chapters, and was guest editor in 2 special issues. Organized 7 events. Supervised / co-supervised (or ongoing) 4 PhD theses, 3 MSc dissertations, and 7 works of course completion of LSc/BSc. Has been involved in 10 projects, of which 3 as the Principal Investigator. Her consolidated expertise in zebrafish animal models, specifically in the areas of anaesthesia, behaviour, and laboratory animal science, allows her to make substantial contributions to improving animal welfare and minimizing the impact of confounding factors in research, such as anaesthesia, analgesia, and husbandry conditions. In addition to her focus on animal welfare, Ana Maria is committed to enhancing the zebrafish model for research purposes. For instance, she is involved in developing behavioural assessments to study SCA37 models and other diseases like diabetes using zebrafish.

Read full resume ↓

Identification

Personal identification

Full name: Ana Maria Marques Valentim

Citation names

Valentim, Ana
Valentim, Ana M

Author identifiers

Ciência ID: 9E18-3854-6BA9
ORCID iD: 0000-0003-1428-0691
Google Scholar ID: 1TyedkUAAAAJ
Researcher Id: C-3389-2013
Scopus Author Id: 24339284100

Email addresses

amaria@ibmc.up.pt (Professional)
amaria@i3s.up.pt (Professional)

Telephones

Telephone

226074900 (Professional)

Addresses

i3S - IBMC. Rua Alfredo Allen, 208, 4200-135, Porto, Porto, Portugal (Professional)

Websites

http://orcid.org/0000-0003-1428-0691 (Professional)

Knowledge fields

Agrarian Sciences - Veterinary Sciences
Medical and Health Sciences - Basic Medicine - Toxicology
Natural sciences - Biological Sciences - Behavioural Sciences Biology

Languages

Language	Speaking	Reading	Writing	Listening	Peer-review
English	Upper intermediate (B2)	Advanced (C1)	Upper intermediate (B2)	Advanced (C1)	Advanced (C1)
Portuguese (Mother tongue)

Education

	Degree	Classification
2023/11 Concluded	Análise e Exploração de Dados em R/ "Analyzing and exploring data in R" (Outros) Universidade do Porto Faculdade de Ciências, Portugal
2023/10 Concluded	Course Building and Leading Research Teams (Outros) Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2022/06/01 - 2022/06/08 Concluded	Research Project Management (Outros) Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2016/06/20 - 2016/06/23 Concluded	International Zebrafish and Medaka Course (Outros) Karlsruher Institut für Technologie, Germany	Approved
2008/02/01 - 2012/03/01 Concluded	Ciências Veterinárias (Doutoramento) Major in PhD in Veterinary Sciences Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal "Behavioural effects of anaesthetics in mice-risk of cognitive alterations " (THESIS/DISSERTATION)	Aproved with distinction
2006/11/20 - 2006/11/24 Concluded	Post-graduate course of Experimental Design and Statistical Methods in Biomedical Experimentation (Outros) Itä-Suomen yliopisto, Finland	Approved
2006/06/12 - 2006/06/23 Concluded	International course in Laboratory Animal Science (Outros) Universiteit Utrecht, Netherlands	Approved
2005 Concluded	Biology (Licenciatura) Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal "Ethological study of a freshwater fish, Cobitis paludica" (THESIS/DISSERTATION)	17 (0-20)
2000 Concluded	Cientifico-Natural (Ensino secundário) Escola Secundária de Tondela, Portugal "none" (THESIS/DISSERTATION)	18 (0-20)

Affiliation

Science

	Category Host institution	Employer
2019/01/02 - Current	Contracted Researcher (Research)	Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal

2015/04/01 - 2018/12/29	Postdoc (Research)	Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal

2013/07/01 - 2015/09/30	Invited Principal Investigator (Research)	Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal

2012/04/02 - 2013/06/30	Postdoc (Research)	Universidade de Trás-os-Montes e Alto Douro, Portugal

2006/02/01 - 2008/01/31	Researcher (Research)	Universidade de Trás-os-Montes e Alto Douro, Portugal

2005/10/03 - 2006/01/31	Researcher (Research)	Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal

2004/10/01 - 2005/06/30	Research Trainee (Research)	Instituto Superior de Psicologia Aplicada Centro de Investigacao, Portugal

Others

	Category Host institution	Employer
2019/08/21 - 2020/01/17	Maternity leave	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal

2008/02/01 - 2012/03/01	PhD student (FCT studentship)	Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal

Projects

Grant

	Designation	Funders
2024 - 2025	Analgesics absorption and elimination: the path to improve prolonged analgesia in zebrafish N/A Principal investigator Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal	American College of Laboratory Animal Medicine Ongoing
2018 - 2022	Pathogenic Mechanisms and Therapy in a Repeat Disease Caused by a Noncoding Mutation in the DAB1 Gene PTDC/MED-GEN/29255/2017 Researcher Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2018/06 - 2021/12/26	ZEBREFINE: Refining zebrafish anesthesia: economical, clinical and animal welfare implications POCI-01-0145-FEDER-029542 Principal investigator Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal	Fundação para a Ciência e a Tecnologia, I.P. Ongoing
2015 - 2018	Optimizing anaesthesia for adult zebrafish – a practical and animal welfare-based approach SFRH/BPD/103006/2014 Post-doc Fellow Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2013/07 - 2015/06	Zebrafish (Danio rerio)anaesthesia and potential implications in research - reduction, replacement and refining techniques/procedures 131642 Provided by PTCRIS: 131642 Principal investigator Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal	Fundação para a Ciência e a Tecnologia, I.P. Concluded
2010/04 - 2013/09	Effects of Ketamine anaesthesia in animal models used on research Provided by PTCRIS: 99022 PTDC/CVT/099022/2008 Post-doc Universidade de Trás-os-Montes e Alto Douro, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2008/02 - 2012/01	BEHAVIOURAL EFFECTS OF ANESTHETICS IN MICE SFRH/BD/36121/2007 PhD Student Fellow	Fundação para a Ciência e a Tecnologia
2006/02 - 2008/01	Refinements in laboratory animals anaesthesia, behavioural and physiological measures Provided by PTCRIS: 59056 Provided by PTCRIS: 59056 Research Fellow Universidade de Trás-os-Montes e Alto Douro, Portugal	Fundação para a Ciência e a Tecnologia Concluded

Contract

	Designation	Funders
2022/01 - Current	Development of interactive e-learning modules on specific areas of the Education & Training framework facilitating implementation of DIR 2010/63/EU ENV/2021/OP/0011 Module expert in "Basic and appropriate biology– zebrafish" and "Recognition of pain, suffering and distress – zebrafish" Universiteit Utrecht, Netherlands	European Commission Ongoing

Outputs

Publications

Book chapter	Ana M. Valentim. "Behavioral Profiling of Zebrafish (Danio rerio) Larvae: Activity, Anxiety, Avoidance, and Startle Response". 2024. 10.1007/978-1-0716-3625-1_26 Köhler, Almut; Valentim, Ana M.. "Analgesia, anesthesia, and euthanasia in zebrafish". In Laboratory Fish in Biomedical Research, 119-137. Elsevier, 2022. 10.1016/b978-0-12-821099-4.00016-x Valentim, Ana M.. "Behavioral Profiling of Zebrafish (Danio rerio) Larvae Following Teratogen Exposure". In Teratogenicity Testing, 403-419. Springer New York, 2018. 10.1007/978-1-4939-7883-0_22
Conference abstract	Castro, Ana Filipa; Ana Figueiredo; Joana Maria Geraldes da Rocha Loureiro; Valentim, Ana M; Jose Carlos Ribeiro Bessa; Isabel Alexandra Azevedo Silveira. "SCA37 (ATTTC)n cell and animal models as valuable resources for therapeutic screening". Paper presented in Final Meeting of COST Action 17103 DARTER Delivery of RNA Antisense Therapeutics, Bilbao, 2023. Ana Filipa Azevedo Castro; Joana Maria Geraldes da Rocha Loureiro; Valentim, Ana M; Jose Carlos Ribeiro Bessa; Isabel Alexandra Azevedo Silveira. "Is the (AUUUC)n insertion RNA enough for motor dysfunction in SCA37? – insights from a zebrafish developmental model". Paper presented in 9th Molecular and Cell Biology Symposium, 2023. Ferreira, Jorge; Inês Caetano; Sofia Barros; Pedro Fernandes; Valentim, Ana M. "Validation of a learning task for TU and AB zebrafish strains". Paper presented in 8th McBiology Symposium, 2022. Ferreira, Jorge; Collins, Mark; Valentim, Ana M. "Anaesthesia induced memory impairment: the silent variable". Paper presented in 8th McBiology Symposium, 2022. Sara Silva Jorge; Jorge Ferreira; Inês Caetano; Valentim, Ana M. "Would social housing of zebrafish (Danio rerio) interfere with the physiological stress response?". Paper presented in IJUP 2022, 2022. Ana Filipa Azevedo Castro; Joana Maria Geraldes da Rocha Loureiro; Jorge Miguel Custódio Martins Ferreira; Valentim, Ana M; Jose Carlos Ribeiro Bessa; Isabel Alexandra Azevedo Silveira. "The spinocerebellar ataxia type 37 ATTTC repeat insertion impacts zebrafish CNS development". Paper presented in Neuroscience 2022, online, 2022. Loureiro, Joana R; Valentim, Ana M; Mafalda Sousa; Paula Sampaio; Silveira, Isabel. "The need for speed in zebrafish behavioural analysis". Paper presented in Euro-BioImaging event: Virtual Pub Speed edition, 2021. Ana Claro; Sara Jorge; Valentim, Ana M. "Behavioural recovery after long-term anaesthesia in zebrafish larvae". Paper presented in IJUP 2021, 2021. Joana Silva; Ferreira, Jorge; Valentim, Ana M. "Behind the scenes of behavioural science in zebrafish: setup and validation of a behavioural task". Paper presented in IJUP, 2020. Jorge Ferreira; Anna I Olsson; Valentim, Ana M. "Anaesthetic efficacy and aversion: Comparing 2 common zebrafish protocols with a combination of propofol/lidocaine". Paper presented in 14th FELASA congress 2019, Prague, 2019. 10.1177/0023677219839199 Ferreira, Jorge; Collins, Mark; Valentim, Ana M. "Refinement of zebrafish anaesthesia – a behavioural and mechanistic approach to study memory alterations". Paper presented in InCell to ExCell - MCBiology Seminar 6th edition, 2019. Jorge M Ferreira; Anna I Olsson; Valentim, Ana M. "Efficacy of different methods of euthanasia in adult zebrafish: anaesthesia overdose and rapid cooling". Paper presented in SPCAL 2018, Braga, 2018. 10.1177/0023677218789822 Jorge M Ferreira; Anna I Olsson; Valentim, Ana M. "Can different depths of anaesthesia influence recognition memory in mice?". Paper presented in SECAL/SPCAL meeting 2015 Abstract book, Caceres, 2015. Published • 10.1177/0023677215609551 Correia, R.; Pereira, AM; Valentim, Ana M; Azevedo, C.; Silva, E.; Gabriel, J; Antunes, LM. "Automatically detect righting reflex loss and respiratory rate during induction of anaesthesia". Paper presented in SECAL/SPCAL meeting 2015, Caceres, 2015. Published • 10.1177/0023677215609551 Antunes, LM; Valentim, Ana M; Guedes, SR; Pereira, AM; Silva, E.; Correia, R.; Gabriel, J. "New developments in gas euthanasia". Paper presented in SECAL/SPECAL meeting 2015, Caceres, 2015. 10.1177/0023677215609551 Monteiro, MP; Félix, Luís Manuel Lourenço; Valentim, Ana M. "Short and long-term effects of ketamine exposure in the development of the animal model zebrafish - first outcomes". Paper presented in Euroanaesthesia 2015, Berlim, 2015. Valentim, Ana M; Magalhães, Ana; Pereira, M.; Venâncio, C.; Summavielle, Teresa; Antunes, LM. "Is ketamine alone and in combination with midazolam or dexmedetomidine safe regarding post-anaesthetic memory?". Paper presented in Euroanaesthesia 2013, Barcelona, 2013. Magalhães, Ana; Valentim, Ana M; Pereira, M.; Melo, P; Venâncio, C.; Summavielle, Teresa; Antunes, LM. "Is ketamine alone or combined with midazolam or dexmedetomidine a good anaesthetic to use in biomedical research that requires behavioural evaluation?". Paper presented in 12th FELASA SECAL Congress, Barcelona, 2013. Published Valentim, Ana M; Anna I Olsson; Antunes, LM. "Influence of anesthetic ketamine/midazolam combination in spatial and motor learning". Paper presented in Anesthesiology annual meeting 2011, Chicago, 2011. Ribeiro, PO; Valentim, Ana M; Rodrigues, PC; Antunes, LM. "Different Concentrations of Ketamine did not Affect Cognition or Neurodegeneration in Adult Mice". Paper presented in Anesthesiology annual meeting 2011, Chicago, 2011. Valentim, Ana M; Anna I Olsson; Antunes, LM. "Ketamine/Midazolam Do Not Interfere with Consolidation and Recall of a Spatial Task in Mice". Paper presented in Anesthesiology annual meeting 2010, San Diego, 2010. Ribeiro, PO; Valentim, Ana M; Rodrigues, PC; Antunes, LM. "Cognitive and Neurodegenerative Effects of the Medetomidine/Ketamine Anesthesia Combination in Mice". Paper presented in Anesthesiology annual meeting 2010, San Diego, 2010. Valentim, Ana M; Di Giminiani, P.; Ribeiro, PO; Anna I Olsson; Antunes, LM. "Depth of Anesthesia Effects on Cognition – a Battery of Behavioral Tests". Paper presented in Anesthesiology Annual meeting 2008, Orlando, 2008. Valentim, Ana M; Alves, HC; Anna I Olsson; Antunes, LM. "Effects of Depth of Isoflurane Anesthesia on Spatial Cognition of Mice". Paper presented in Anesthesiology annual meeting 2007, San Francisco, 2007. Alves, HC; Valentim, Ana M; Anna I Olsson; Antunes, LM. "Effects of medetomidine and fentanyl on propofol requirements for loss of consciousness in mice". Paper presented in Anesthesiology annual meeting 2007, San Francisco, 2007. Valentim, Ana M; Alves, HC; Anna I Olsson; Antunes, LM. "Lower isoflurane concentrations may have a stronger effect than higher concentrations in spatial learning in mice". Paper presented in Euroanaesthesiology 2007, Munich, 2007. Alves, HC; Valentim, Ana M; Anna I Olsson; Antunes, LM. "Effects of propofol combined with fentanyl, sulfentanil or remifentanil in mice ananesthesia by intraperitoneal route". Paper presented in Euroanaesthesia 2006, Madrid, 2007. Alves, HC; Valentim, Ana M; Alves, M.; Anna I Olsson; Antunes, LM. "Synergic effects between alpha2 agonist (medetomidine) and fentanyl with dissociative (tiletamine) and zolazepam in rats anaesthesia". Paper presented in Euroanaesthesia 2007, Madrid, 2007.
Conference paper	Pereira, A.M.; Antunes, L.; Valentim, A.M.; Correia, R.; Gabriel, J.. "Development of a respiratory rate monitoring device for mice anesthesia induction chamber". 2014. 10.1109/MeMeA.2014.6860037
Conference poster	Jorge, Sara; Félix, Luís Manuel Lourenço; Costas, Benjamin; Valentim, Ana. "DIVING INTO ZEBRAFISH STRESS: SKIN MUCUS CORTISOL AS A STRESS BIOMARKER". Paper presented in Aquaculture Europe 2023, 2023. Santos, C.A.; Félix, Luís Manuel Lourenço; Valentim, Ana M; Antunes, Luis Miguel Joaquim Marques; Pinto, Maria de Lurdes. Corresponding author: Pinto, Maria de Lurdes. "Effects of ketamine administration in the expression of Sox 2 in zebrafish CNS". Paper presented in Joint congress of Veterinary Pathology and Veterinary Clinical Pathology, 2023. Santos, C.; Valentim, Ana M; Félix, Luís; Antunes, Luis Miguel Joaquim Marques; Pinto, Maria de Lurdes. Corresponding author: Pinto, Maria de Lurdes. "Ketamine administration at the 1-4 somites stage zebrafish does not alter the distribution patterns of serotonin 5-ht-2b receptor in adult animals". Paper presented in Joint congress of Veterinary Pathology and Veterinary Clinical Pathology, 2023. Marques, Joana; Teixeira, Joana; Cadete, Francisco; Duque, Marta; Valentim, Ana M; Bessa, Jose.. "Modelling the crosstalk of diabetes and anxiety in zebrafish". Paper presented in 12th European Zebrafish Meeting, Krakow, Poland, 2023. Santos, C.; Félix, Luís Manuel Lourenço; Valentim, Ana; Pinto, MLr. "Central nervous system atypical Sox 2 expression following ketamine exposure in zebrafish". Paper presented in 2ndMeeting of the Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), 2023. Sara Silva Jorge; Luís Félix; Benjamin Costas Refojos; Valentim, Ana M. "ZMATRIX PROJECT - Changing science and zebrafish welfare one step at a time". Paper presented in Beyond REMODEL: 3R models for biomedical research, 2023. Sara Silva Jorge; Ana Claro; Luís Félix; Valentim, Ana M. "The challenge of long duration anaesthesia for zebrafish larvae". Paper presented in FELASA 2022, 2022. 10.1177/00236772221103950 Castro, Ana Filipa; Loureiro, Joana R; Valentim, Ana M; Bessa, Jose; Silveira, Isabel. "(AUUUC)n zebrafish developmental model: a tool to study the SCA37 pathogenic mechanism". Paper presented in 8th McBiology Symposium, 2022. Jorge, Sara; Valentim, Ana M; et al.. "Protocol for acute stress induction in adult zebrafish - a validation study". Paper presented in 8th McBiology Symposium, 2022. Jorge Miguel Custódio Martins Ferreira(C312-4F71-3635); Ingrid Anna Sofia Olsson; Valentim, Ana M. "The importance of behavioural tasks terminology: methodological evidence". Paper presented in 54th Congress of the ISAE, 2021. Sara Silva Jorge; Jorge Miguel Custódio Martins Ferreira; Ingrid Anna Sofia Olsson; Valentim, Ana M. "Are anaesthetics affecting zebrafish behaviour? - a post-recovery study". Paper presented in Zebrafish 2020 virtual meeting, 2020. Rita Correia; Rita Sequeira; Catarina Ribeiro; Luís Félix; Sandra Mariza Veiga Monteiro; Luis Antunes; Valentim, Ana M; Carlos Venâncio. "Improving fish welfare in transport using MS-222 or propofol as sedative". Paper presented in AquaImprove: 4th Aquaculture Research Workshop, 2019. Catarina Ribeiro; Rita Correia; Rita Sequeira; Luís Félix; Luís Antunes; Valentim, Ana M; Carlos Venâncio; Sandra Mariza Veiga Monteiro. "Effect of tricaine mesylate (MS-222) and propofol on Nile Tilapia (Oreochromis niloticus) gill after transport stimulation". Paper presented in AquaImprove: 4th Aquaculture Research Workshop, 2019. Rita Sequeira; Rita Correia; Catarina Ribeiro; Carlos Venâncio; Sandra Mariza Veiga Monteiro; Luís Antunes; Valentim, Ana M; Luís Félix. "Oxidative status in the brain and liver of Nile tilapia sedated with propofol and MS-222 under simulated transport conditions". Paper presented in ”, AquaImprove: 4th Aquaculture Research Workshop, 2019. Rita Correia; Rita Sequeira; Catarina Ribeiro; Luís Félix; Sandra Mariza Veiga Monteiro; Luís Antunes; Valentim, Ana M; Carlos Venâncio. "Fish welfare: sedative effects of MS222 and propofol under simulated transport conditions". Paper presented in 11th International Symposium on Wild Fauna, 2019. Jorge Miguel Custódio Martins Ferreira(C312-4F71-3635); Ingrid Anna Sofia Olsson; Valentim, Ana M. "A less aversive anaesthetic to adult zebrafish". Paper presented in UFAW International Animal Welfare Science Symposium, 2017. Valentim, Ana M; et al. "Zebrafish (Danio rerio) aversion to MS222 and propofol/lidocaine anaesthesia". Paper presented in XIII Congresso Sociedade Portuguesa de Etologia, 2016. Valentim, Ana M; et al. "Zebrafish as a development model for learning and anxiety alterations induced by ketamine". Paper presented in International FishMed Conference on Zebrafish Research, 2016. Valentim, Ana M; et al. "Ketamine long-term effects on learning and anxiety of zebrafish.". Paper presented in AAALAC International, ESLAV and ECLAM Conference, 2015. Valentim, Ana M; et al. "Mice aversion to the gases isoflurane, sevoflurane and CO2". Paper presented in 54th CALAS/ACSAL Symposium, 2015. Valentim, Ana M; et al. "Automatic detection of righting reflex loss in laboratory rodents with piezoelectric sensors". Paper presented in 54th CALAS/ACSAL Symposium, 2015. Valentim, Ana M; et al. "Assessment of central nervous cell proliferation ability in Zebrafish embryos treated with ketamine". Paper presented in Biomarcadores em Ciência Animal e Ciências Veterinárias: Uma abordagem Interdisciplinar, 2015. Valentim, Ana M; et al. "A pilot study about alternatives for adult zebrafish anesthesia". Paper presented in Joint HSBLAS/ESLAV/ECLAM Meeting, 2014. Valentim, Ana M; Olsson, I A S. "Zebrafish model on the upswing - a behavioural review". Paper presented in X Congresso Nacional de Etologia, 2013. Valentim, Ana M; et al. "Anaesthesia as a variable or as a refinement". Paper presented in ASAB/SEB/NC3Rs Symposium, 2012. Valentim, Ana M; et al. "Is the common used anaesthetic isoflurane interfering with behavioural results?". Paper presented in II SPCAL meeting, 2012. Patricia Ribeiro; Valentim, Ana M; et al. "Cognitive and neurodegenerative effects of the medetomidine/ketamine anesthesia combination in adult mice". Paper presented in SCAND-FELASA Joint Meeting, 2010. Patricia Ribeiro; Valentim, Ana M; et al. "Histological and behavioural effects of medetomidine/ketamine anaesthesia in mice". Paper presented in AVA meeting, 2008. Héber Alves; Valentim, Ana M; et al. "Tiletamine – Zolazepam - Medetomidine - Fentanyl anaesthesia combination in rats". Paper presented in FELASA-ICLAS Joint Meeting, 2007. Héber Alves; Valentim, Ana M; et al. "Propofol- Medetomidine and Fentanyl anaesthesia combination in mice". Paper presented in FELASA-ICLAS Joint Meeting, 2007.
Journal article	Jorge, Sara; Félix, Luís; Costas, Benjamín; Valentim, Ana M.. "Advancing cortisol measurements in zebrafish: Analytical validation of a commercial ELISA kit for skin mucus cortisol analysis". MethodsX 12 (2024): 102726. http://dx.doi.org/10.1016/j.mex.2024.102726. 10.1016/j.mex.2024.102726 Beatriz Custódio; Patrícia Carneiro; Joana Marques; Victoria Leiro; Ana M. Valentim; Mafalda Sousa; Sofia D. Santos; José Bessa; Ana P. Pêgo. "Biological Response Following the Systemic Injection of PEG–PAMAM–Rhodamine Conjugates in Zebrafish". Pharmaceutics (2024): https://doi.org/10.3390/pharmaceutics16050608. 10.3390/pharmaceutics16050608 Santos, C.; Valentim, A.M.; Félix, L.; Balça-Silva, J.; Pinto, M.L.. "Longitudinal effects of ketamine on cell proliferation and death in the CNS of zebrafish". NeuroToxicology 97 (2023): 78-88. http://dx.doi.org/10.1016/j.neuro.2023.05.008. Published • 10.1016/j.neuro.2023.05.008 Sara Jorge; Luís Félix; Benjamín Costas; Ana M. Valentim. "Housing Conditions Affect Adult Zebrafish (Danio rerio) Behavior but Not Their Physiological Status". Animals (2023): https://doi.org/10.3390/ani13061120. 10.3390/ani13061120 Jorge M. Ferreira; Sara Jorge; Luís Félix; Gabriela M. Morello; I. Anna S. Olsson; Ana M. Valentim. "Behavioural Aversion and Cortisol Level Assessment When Adult Zebrafish Are Exposed to Different Anaesthetics". Biology (2022): https://doi.org/10.3390/biology11101433. 10.3390/biology11101433 Jorge M. Ferreira; Luís Félix; Sara Jorge; Sandra M. Monteiro; I. Anna S. Olsson; Ana M. Valentim. "Anesthesia Overdose Versus Rapid Cooling for Euthanasia of Adult Zebrafish". Zebrafish (2022): https://doi.org/10.1089/zeb.2022.0001. 10.1089/zeb.2022.0001 Luís Félix; Rita Correia; Rita Sequeira; Cristiana Ribeiro; Sandra Monteiro; Luís Antunes; José Silva; Carlos Venâncio; Ana Valentim. "MS-222 and Propofol Sedation during and after the Simulated Transport of Nile tilapia (Oreochromis niloticus)". Biology (2021): https://doi.org/10.3390/biology10121309. 10.3390/biology10121309 Valentim, Ana M.; Gaburro, Stefano; Parker, Matthew O.. "Editorial: Post-anesthesia Cognitive Dysfunction: How, When and Why". Frontiers in Behavioral Neuroscience 15 (2021): http://dx.doi.org/10.3389/fnbeh.2021.797483. Published • 10.3389/fnbeh.2021.797483 Sara Jorge; Jorge M. Ferreira; I. Anna S. Olsson; Ana M. Valentim. "Adult Zebrafish Anesthesia: A Study of Efficacy and Behavioral Recovery of Different Anesthetics". Zebrafish (2021): https://doi.org/10.1089/zeb.2021.0023. 10.1089/zeb.2021.0023 Félix, Luís M.; Luzio, Ana; Antunes, Luís; Coimbra, Ana M.; Valentim, Ana M.. "Malformations and mortality in zebrafish early stages associated with elevated caspase activity after 24 h exposure to MS-222". Toxicology and Applied Pharmacology 412 (2021): 115385. http://dx.doi.org/10.1016/j.taap.2020.115385. 10.1016/j.taap.2020.115385 Félix, L.M.; Luzio, A.; Santos, A.; Antunes, L.M.; Coimbra, A.M.; Valentim, A.M.. "MS-222 induces biochemical and transcriptional changes related to oxidative stress, cell proliferation and apoptosis in zebrafish embryos". Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology 237 108834 (2020): http://www.scopus.com/inward/record.url?eid=2-s2.0-85087124131&partnerID=MN8TOARS. 10.1016/j.cbpc.2020.108834 Luís Félix; Ana Maria Coimbra; Ana Maria Valentim; Luís Antunes. "Review on the use of zebrafish embryos to study the effects of anesthetics during early development". Critical Reviews in Toxicology (2019): 1-14. https://doi.org/10.1080/10408444.2019.1617236. 10.1080/10408444.2019.1617236 Santos, A.; Balça-Silva, J.; Félix, L.; Valentim, A.M.; Pires, M.A.; Gonçalves, C.; Pinto, M.L.. "Effects of ketamine exposure in zebrafish central nervous system proliferation capability". Journal of Comparative Pathology 166 (2019): 121. http://dx.doi.org/10.1016/j.jcpa.2018.10.067. 10.1016/j.jcpa.2018.10.067 Martins T; Valentim A; Pereira N; Antunes LM. "Anaesthetics and analgesics used in adult fish for research: A review.". Laboratory animals (2018): http://europepmc.org/abstract/med/30514148. 10.1177/0023677218815199 Luís M. Félix; Ana Luzio; Maria Themudo; Luís Antunes; Manuela Matos; Ana M. Coimbra; Ana M. Valentim. "MS-222 short exposure induces developmental and behavioural alterations in zebrafish embryos". Reproductive Toxicology 81 (2018): 122-131. https://doi.org/10.1016/j.reprotox.2018.07.086. 10.1016/j.reprotox.2018.07.086 Sneddon, Lynne U.; Lopez-Luna, Javier; Wolfenden, David C.C.; Leach, Matthew C.; Valentim, Ana M.; Steenbergen, Peter J.; Bardine, Nabila; et al. "Fish sentience denial: Muddying the waters". Animal Sentience 3 21 (2018): http://dx.doi.org/10.51291/2377-7478.1317. 10.51291/2377-7478.1317 Sneddon, Lynne U.; Wolfenden, David C.C.; Leach, Matthew C.; Valentim, Ana M.; Steenbergen, Peter J.; Bardine, Nabila; Broom, Donald M.; Brown, Culum. "Ample evidence for fish sentience and pain". Animal Sentience 3 21 (2018): http://dx.doi.org/10.51291/2377-7478.1375. 10.51291/2377-7478.1375 Félix, L.M.; Vidal, A.M.; Serafim, C.; Valentim, A.M.; Antunes, L.M.; Monteiro, S.M.; Matos, M.; Coimbra, A.M.. "Ketamine induction of p53-dependent apoptosis and oxidative stress in zebrafish (Danio rerio) embryos". Chemosphere 201 (2018): 730-739. http://www.scopus.com/inward/record.url?eid=2-s2.0-85043517573&partnerID=MN8TOARS. 10.1016/j.chemosphere.2018.03.049 Sneddon, Lynne U.; Lopez-Luna, Javier; Wolfenden, David C. C.; Leach, Matthew C.; Valentim, Ana M.; Steenbergen, Peter J.; Bardine, Nabila; et al. "Response to: Responses of larval zebrafish to low pH immersion assay. Comment on Lopez-Luna et al.". Journal of Experimental Biology 220 17 (2017): 3192-3194. http://dx.doi.org/10.1242/jeb.163451. 10.1242/jeb.163451 Magalhães, A.; Valentim, A.; Venâncio, C.; Pereira, M.; Melo, P.; Summavielle, T.; Antunes, L.. "Ketamine alone or combined with midazolam or dexmedetomidine does not affect anxiety-like behaviours and memory in adult wistar rats". Laboratory Animals 51 2 (2017): 147-159. http://www.scopus.com/inward/record.url?eid=2-s2.0-85018396469&partnerID=MN8TOARS. 10.1177/0023677216652380 Félix, L.M.; Serafim, C.; Valentim, A.M.; Antunes, L.M.; Matos, M.; Coimbra, A.M.. "Apoptosis-related genes induced in response to ketamine during early life stages of zebrafish". Toxicology Letters 279 (2017): 1-8. http://www.scopus.com/inward/record.url?eid=2-s2.0-85025172761&partnerID=MN8TOARS. 10.1016/j.toxlet.2017.07.888 Antunes, L.M.; Coimbra, A.M.; Valentim, A.M.. "Behavioral alterations of zebrafish larvae after early embryonic exposure to ketamine". Psychopharmacology 234 4 (2017): 549-558. http://www.scopus.com/inward/record.url?eid=2-s2.0-85001555685&partnerID=MN8TOARS. 10.1007/s00213-016-4491-7 Félix, L.M.; Serafim, C.; Martins, M.J.; Valentim, A.M.; Antunes, L.M.; Matos, M.; Coimbra, A.M.. "Morphological and behavioral responses of zebrafish after 24 h of ketamine embryonic exposure". Toxicology and Applied Pharmacology 321 (2017): 27-36. http://www.scopus.com/inward/record.url?eid=2-s2.0-85013752088&partnerID=MN8TOARS. 10.1016/j.taap.2017.02.013 Guedes, S.R.; Valentim, A.M.; Antunes, L.M.. "Mice aversion to sevoflurane, isoflurane and carbon dioxide using an approach-avoidance task". Applied Animal Behaviour Science 189 (2017): 91-97. http://www.scopus.com/inward/record.url?eid=2-s2.0-85011556791&partnerID=MN8TOARS. 10.1016/j.applanim.2017.01.012 Köhler, A.; Collymore, C.; Finger-Baier, K.; Geisler, R.; Kaufmann, L.; Pounder, K.C.; Schulte-Merker, S.; et al. "Report of workshop on euthanasia for zebrafish - a matter of welfare and science". Zebrafish 14 6 (2017): 547-551. http://www.scopus.com/inward/record.url?eid=2-s2.0-85036516157&partnerID=MN8TOARS. 10.1089/zeb.2017.1508 Valentim, A.M.; van Eeden, F.J.; Strähle, U.; Olsson, I.A.S.. "Euthanizing zebrafish legally in Europe: Are the approved methods of euthanizing zebrafish appropriate to research reality and animal welfare?". EMBO Reports 17 12 (2016): 1688-1689. http://www.scopus.com/inward/record.url?eid=2-s2.0-84995451033&partnerID=MN8TOARS. 10.15252/embr.201643153 Valentim, A.M.; Guedes, S.R.; Pereira, A.M.; Antunes, L.M.. "Euthanasia using gaseous agents in laboratory rodents". Laboratory Animals 50 4 (2016): 241-253. http://www.scopus.com/inward/record.url?eid=2-s2.0-84986557845&partnerID=MN8TOARS. 10.1177/0023677215618618 Félix, L.M.; Serafim, C.; Valentim, A.M.; Antunes, L.M.; Campos, S.; Matos, M.; Coimbra, A.M.. "Embryonic Stage-Dependent Teratogenicity of Ketamine in Zebrafish (Danio rerio)". Chemical Research in Toxicology 29 8 (2016): 1298-1309. http://www.scopus.com/inward/record.url?eid=2-s2.0-84982279770&partnerID=MN8TOARS. 10.1021/acs.chemrestox.6b00122 Martins, T.; Valentim, A.M.; Pereira, N.; Antunes, L.M.. "Anaesthesia and analgesia in laboratory adult zebrafish: A question of refinement". Laboratory Animals 50 6 (2016): 476-488. http://www.scopus.com/inward/record.url?eid=2-s2.0-85014491998&partnerID=MN8TOARS. 10.1177/0023677216670686 Valentim, A.M.; Félix, L.M.; Carvalho, L.; Diniz, E.; Antunes, L.M.. "A new anaesthetic protocol for adult zebrafish (Danio rerio): Propofol combined with lidocaine". PLoS ONE 11 1 (2016): http://www.scopus.com/inward/record.url?eid=2-s2.0-84959205162&partnerID=MN8TOARS. 10.1371/journal.pone.0147747 Luís M. Félix; Ana M. Vidal; Cindy Serafim; Ana M. Valentim; Luís M. Antunes; Sónia Campos; Manuela Matos; Sandra M. Monteiro; Ana M. Coimbra. "Ketamine-induced oxidative stress at different developmental stages of zebrafish (Danio rerio) embryos". RSC Adv. 6 66 (2016): 61254-61266. http://dx.doi.org/10.1039/c6ra08298j. 10.1039/c6ra08298j Santos, Carla; Félix, L.; Neves, M.; Coimbra, A.; Valentim, A.M.; Antunes, L.; Gonçalves, C.; et al. "Refinement Techniques in Zebrafish Anaesthesia – Results from a Pilot Study". Microscopy and Microanalysis 21 S5 (2015): 93-94. http://dx.doi.org/10.1017/s1431927615014270. 10.1017/s1431927615014270 Ribeiro, P.O.; Rodrigues, P.C.; Valentim, A.M.; Antunes, L.M.. "A single intraperitoneal injection of ketamine does not affect spatial working, reference memory or neurodegeneration in adult mice: An animal study". European Journal of Anaesthesiology 30 10 (2013): 618-626. http://www.scopus.com/inward/record.url?eid=2-s2.0-84884480706&partnerID=MN8TOARS. 10.1097/EJA.0b013e3283610321 Valentim, A.M.; Ribeiro, P.O.; Olsson, I.A.S.; Antunes, L.M.. "The memory stages of a spatial Y-maze task are not affected by a low dose of ketamine/midazolam". European Journal of Pharmacology 712 1-3 (2013): 39-47. http://www.scopus.com/inward/record.url?eid=2-s2.0-84878219774&partnerID=MN8TOARS. 10.1016/j.ejphar.2013.04.027 Valentim, A.M.; Olsson, I.A.S.; Antunes, L.M.. "The anaesthetic combination of ketamine/midazolam does not alter the acquisition of spatial and motor tasks in adult mice". Laboratory Animals 47 1 (2013): 19-25. http://www.scopus.com/inward/record.url?eid=2-s2.0-84878042050&partnerID=MN8TOARS. 10.1258/la.2012.011179 Ribeiro, P.O.; Valentim, A.M.; Rodrigues, P.; Olsson, I.A.; Antunes, L.M.. "Apoptotic neurodegeneration and spatial memory are not affected by sedative and anaesthetics doses of ketamine/medetomidine combinations in adult mice". British Journal of Anaesthesia 108 5 (2012): 807-814. http://www.scopus.com/inward/record.url?eid=2-s2.0-84859840810&partnerID=MN8TOARS. 10.1093/bja/aes003 Valentim, A.M.; Summavielle, T.; Antunes, L.M.. "Importance of body temperature and clinical data in behavioral and anesthesia studies". Anesthesiology 116 1 (2012): 226-227. http://www.scopus.com/inward/record.url?eid=2-s2.0-84555218001&partnerID=MN8TOARS. 10.1097/ALN.0b013e31823cf056 Valentim, A.M.; Di Giminiani, P.; Ribeiro, P.O.; Rodrigues, P.; Olsson, I.A.S.; Antunes, L.M.. "Lower isoflurane concentration affects spatial learning and neurodegeneration in adult mice compared with higher concentrations". Anesthesiology 113 5 (2010): 1099-1108. http://www.scopus.com/inward/record.url?eid=2-s2.0-78049352411&partnerID=MN8TOARS. 10.1097/ALN.0b013e3181f79c7c Alves, H.C.; Valentim, A.M.; Olsson, I.A.S.; Antunes, L.M.. "Intraperitoneal anaesthesia with propofol, medetomidine and fentanyl in mice". Laboratory Animals 43 1 (2009): 27-33. http://www.scopus.com/inward/record.url?eid=2-s2.0-58349101546&partnerID=MN8TOARS. 10.1258/la.2008.007036 Valentim, A.M.; Alves, H.C.; Olsson, I.A.S.; Antunes, L.M.. "The effects of depth of isoflurane anesthesia on the performance of mice in a simple spatial learning task". Journal of the American Association for Laboratory Animal Science 47 3 (2008): 16-19. http://www.scopus.com/inward/record.url?eid=2-s2.0-44649149078&partnerID=MN8TOARS. Valentim, A.M.; Alves, H.C.; Silva, A.M.; Olsson, I.A.S.; Antunes, L.M.. "Effects of depth of isoflurane anaesthesia on a cognition task in mice". British Journal of Anaesthesia 101 3 (2008): 434-435. http://www.scopus.com/inward/record.url?eid=2-s2.0-50949121694&partnerID=MN8TOARS. 10.1093/bja/aen228 Alves, H.C.; Valentim, A.M.; Olsson, I.A.S.; Antunes, L.M.. "Intraperitoneal propofol and propofol fentanyl, sufentanil and remifentanil combinations for mouse anaesthesia". Laboratory Animals 41 3 (2007): 329-336. http://www.scopus.com/inward/record.url?eid=2-s2.0-34547617862&partnerID=MN8TOARS. 10.1258/002367707781282767
Journal issue	Félix, Luís Manuel Lourenço; Valentim, Ana M; Carlos Venâncio. "Research Topic: Efficacy and effects of aquatic animal anaesthesia". Frontiers in Veterinary Science (2024): https://www.frontiersin.org/research-topics/60921/efficacy-and-effects-of-aquatic-animal-anaesthesia. Under revision • Invited editor Valentim, Ana M; Sneddon, Lynne; Robyn Crook. "Special Issue "Anaesthetics and Analgesics Used in Aquatic Animals"". Biology (2023): https://www.mdpi.com/journal/biology/special_issues/AAUAA. Published • Invited editor Valentim, Ana M; Gaburro, Stefano; Parker, Matthew O.. "Research Topic Post-anesthesia Cognitive Dysfunction: How, When and Why". Frontiers in Behavioral Neuroscience (2021): https://www.frontiersin.org/research-topics/16916/post-anesthesia-cognitive-dysfunction-how-when-and-why#overview. Published • Invited editor
Preprint	Sara Jorge; Luís Félix; Benjamín Costas; Ana M Valentim. "Housing conditions affect adult zebrafish (Danio rerio) behavior but not their physiological status". 2023. https://doi.org/10.1101/2023.02.10.528020. 10.1101/2023.02.10.528020 Ferreira, Jorge Miguel; Olsson, I. Anna S.; Valentim, Ana Maria. "Disentangling the terminology of the light/ dark and white/ black test in zebrafish". 2023. http://dx.doi.org/10.31219/osf.io/f9hmq. 10.31219/osf.io/f9hmq Jorge, Sara; Ferreira, Jorge M; Olsson, I Anna S; Valentim, Ana M. "Adult zebrafish anaesthesia: a study of efficacy and behavioural recovery of different anaesthetics". 2021. http://dx.doi.org/10.1101/2021.02.23.432432. 10.1101/2021.02.23.432432 Ferreira, Jorge M; Valentim, Ana M. "Lighter compared to deeper isoflurane anaesthesia can affect recognition memory in male C57BL/6 mice". 2019. http://dx.doi.org/10.1101/645051. 10.1101/645051 Ferreira, Jorge M; Olsson, I Anna S; Valentim, Ana M. "Adult zebrafish euthanasia: efficacy of anaesthesia overdose versus rapid cooling". 2018. http://dx.doi.org/10.7287/peerj.preprints.27432v1. 10.7287/peerj.preprints.27432v1

Activities

Oral presentation

	Presentation title	Event name Host (Event location)
2024/01	Navigating into the 2R’s of zebrafish research: refinement and reduction examples	V SPCAL congress Sociedade Portuguesa de Animais de Laboratório (Coimbra, Portugal)
2023/12/13	Zebrafish Anaesthesia: A Tail of Refinement and Side-Effects	Pain and Analgesia in Zebrafish and Rodent Models Bioresearch & Veterinary Services, University of Edinburgh (Edinburgh )
2023/11/28	Analgesia in fish - situation point	SGV's (Swiss Laboratory Animal Science Association) annual meeting Swiss Laboratory Animal Science Association (Zurich, Switzerland)
2023/04	3Rs and Zebrafish: a swim through our work as laboratory animal scientists	Beyond REMODEL: 3R models for biomedical research i3S – Instituto de Investigação e Inovação em Saúde in Porto, Portugal (Portugal)
2022/06	Anaesthesia induced memory impairment: the silent variable	FELASA 2022 (France)
2022/05	ZEBREFINE - a Portuguese project to refine zebrafish anaesthesia	Spain and Portugal animal welfare, model refinement and advocacy webinar: technology and science Spanish National Research Council (online)
2022/02/23	Swimming towards better anaesthetic protocols in zebrafish	Focus on zebrafish - practical refinements for fish in research and testing Royal Society for the Prevention of Cruelty to Animals (RSPCA ) (online, United Kingdom)
2022/01/27	Which anaesthetics can be used for long-duration anaesthesia in zebrafish larvae, and Anaesthetic overdose vs rapid cooling to euthanise adult zebrafish	ZEBREFINE - refinement opportunities for zebrafish anaesthesia i3S – Instituto de Investigação e Inovação em Saúde in Porto, Portugal (online)
2022	Taking the stress out of zebrafish	Zebrafish Husbandry Association Euro meeting The Francis Crick Institute (London, United Kingdom)
2022	A 3D-printed Do It Yourself approach to memory assessment for adult zebrafish	Zebrafish Husbandry Association Euro meeting The Francis Crick Institute (London, United Kingdom)
2021/11	"Efeitos toxicológicos da anestesia em peixe-zebra" - webinar
2021	Long duration anaesthesia in zebrafish larvae	XVIII Conference of the Portuguese Society of Ethology- SPE 2021 (online)
2021	Open-source behavioural apparatus for memory assessment for the AB and TU zebrafish strain	XVIII Conference of the Portuguese Society of Ethology- SPE 2021 Luso-brasilian organization (online)
2020/11/27	"Efeitos toxicológicos da anestesia em peixe-zebra" - webinar	Escola Universitária Vasco da Gama (online, Portugal)
2018/11	Protocols' comparison to zebrafish anaesthesia and euthanasia	Nordic Zebrafish and Husbandry meeting 2018 Karolinska Institute (Stockholm, Sweden)
2017/08	Avaliação de doenças nos roedores Limites críticos e eutanásia	IV Symposium of "Bioterismo do Einstein" Instituto Einstein (São Paulo, Brazil)
2016/04	Anaesthesia of laboratory animals: considering refinement and research	I Jornadas do Programa de Doutoramento em Ciências Veterinárias ICBAS, University of Porto (Porto, Portugal)
2016/02	Zebrafish: an upswing model in research and the need for refinement of anaesthesia	International Laboratory Animal Science (LAS) virtual conference Labroots
2015/10	Optimization of the object recognition task protocol in C57BL/6 mice	XII Congresso Sociedade Portuguesa de Etologia ISPA (Lisbon, Portugal)
2015/06	New developments in gas euthanasia	. 54th CALAS/ACSAL Symposium (Montréal, Canada)
2014/09	New anaesthesia protocols in zebrafish - pilot study	52nd Annual Meeting GV-SOLAS (Frankfurt, Germany)
2014/04	Session "A Ciência em Portugal" (The Science in Portugal)	I Jornadas de Bioquímica University of Porto (Porto, Portugal)
2012/11	Potential Artifacts Induced by Anesthesia in Rodent Models, Special Topic Lecture	63rd AALAS National Meeting (Minneapolis, United States)
2010/06	No effect of ketamine/midazolam anaesthesia in mice on consolidation or recall of a spatial task	SCAND-FELASA Joint Meeting (Helsinki, Finland)
2006/06	"Aspectos etológicos e de bem-estar animal em testes de memória e aprendizagem aplicados a roedores" (Discussion of some topics from learning and memory behavioural tests in rodents)	Congresso Nacional de Etologia Universidade de Coimbra (Coimbra, Portugal)

Supervision

	Thesis Title Role	Degree Subject (Type) Institution / Organization
2023 - Current	Antisense repeat expression in spinocerebellar ataxia type 37 with implications for therapy Co-supervisor of Ana Filipa Azevedo Castro	Biologia Molecular e Celular (PhD) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2021/02/01 - Current	Establishment of different matrices to measure cortisol in zebrafish under acute and chronic stressful situations Supervisor of Sara Jorge	Ciências Veterinárias (PhD) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2021 - Current	Modelling the cross-talk of diabetes and emotional disorders comorbidities in zebrafish for improvement of therapeutic strategies and animal welfare Co-supervisor of Joana Marques	Programa Doutoral em Biologia Molecular e Celular (PhD) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2019/01/02 - Current	Refinement of zebrafish anaesthesia – a behavioural and mechanistic approach to study memory alterations Supervisor of Jorge Miguel Ferreira	Programa Doutoral em Biologia Molecular e Celular (PhD) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2023/11 - 2024/06	Refinement of analgesic protocol in zebrafish Supervisor	Applied Science (Degree) School of Engineering and Applied Science, Rotterdam University of Applied Science, Netherlands Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2021/10 - 2022/12	Behavioural and physiological assessment of socially isolated Zebrafish (Danio rerio) Supervisor of Inês Caetano	Ciências do Mar - Recursos Marinhos (Master) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2022/02 - 2022/09	Preference test for environmental enrichment of laboratory adult zebrafish Supervisor of Maria Inês Amaral	Ciências do Meio Aquático (Degree) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2021/02/01 - 2021/02/28	Manutenção de um biotério de peixe-zebra e cuidados a ter com os animais Supervisor	Prática Veterinária Integrada (Other) Escola Universitária Vasco da Gama, Portugal
2020/11 - 2021	The influence of anaesthesia on zebrafish (Danio rerio) memory Supervisor	Biologia (Degree) Universidade do Porto Faculdade de Ciências, Portugal
2020/11 - 2021	Effects of anaesthesia induction and recovery on zebrafish (Danio rerio) larvae behaviour Supervisor	Biologia (Degree) Universidade do Porto Faculdade de Ciências, Portugal
2019/10 - 2020/07	Setting up and validating a behavioural task to test aversive learning in zebrafish Supervisor of Joana Silva	Biologia (Degree) Universidade do Porto Faculdade de Ciências, Portugal
2015 - 2016	Anaesthetic aversion in zebrafish Supervisor	Ecologia, Ambiente e Território (Master) Universidade do Porto Faculdade de Ciências, Portugal
2014 - 2015	Potential Implications of Ketamine Anesthesia on Zebrafish Behavior Co-supervisor of Margarida Pinto Monteiro	Psicologia (Master) Universidade do Minho, Portugal
2014 - 2015	Object recognition task in mice Supervisor of Margarida Pinto Monteiro	Basic processes in Psychology (Degree) Universidade do Minho, Portugal
2013 - 2014	The effects of isoflurane anaesthesia depth on the performance of mice in a non-spatial memory task Supervisor of Jorge Miguel Custódio Martins Ferreira	Biologia (Degree) Universidade do Porto Faculdade de Ciências, Portugal
2012 - 2013	"Implicações da cetamina e suas combinações anestésicas nos níveis de ansiedade no rato Wistar" - Implications of ketamine and its anaesthetic combinations on the anxiety levels of the Wistar rats Co-supervisor of Mariana Pereira	Neurofisiologia (Degree) Instituto Politécnico do Porto Escola Superior de Saúde, Portugal

Event organisation

	Event name Type of event (Role)	Institution / Organization
2014 - Current	Junior University: outreach activity consisting in a summer school to high school students to contact with how research is done, in particular in the area of laboratory animal science with the focus on animal behaviour. (2016) Other (Co-organisor)	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2021/11 - 2022/01	ZEBREFINE - refinement opportunities for zebrafish anaesthesia: presentation of the ZEBREFINE project outputs, and invited talks from the scientific community about the challenges of anaesthesia refinement. (https://www.i3s.up.pt/event?v=200) (2022/01/27 - 2022/01/27) Meeting (Member of the Organising Committee)	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2020/11 - 2021/10	Wellbeing and Mental Health at Work: a thematic cycle of seminars and workshops, aiming to promote wellbeing and mental health in the i3S work context. (2021/03 - 2021/10) Workshop (Member of the Organising Committee)	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2018 - 2019	Building and Leading Research Teams (2019/01 - 2019/01) Workshop (Co-organisor)	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal Universidade do Porto Faculdade de Psicologia e de Ciências da Educação, Portugal
2018 - 2018	Session "Career development- beyond the scientific skills" IN Annual Retreat of i3S (2018 - 2018) Other (Co-organisor)	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2017 - 2018	Career development opportunities for researchers and PhD students: effective communication skills and career guidance (2018/01 - 2018/01) Workshop (Co-organisor)	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2017 - 2018	Career development workshop for researchers in the biosciences (2018/12 - 2018/12) Workshop (Co-organisor)	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2016 - 2017	Writing your way into the right job - a CV skills workshop (2017/05 - 2017/05) Workshop (Co-organisor)	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2015 - 2016	Careers in science & beyond (2016 - 2016) Conference (Co-organisor)	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal

Event participation

	Activity description Type of event	Event name Institution / Organization
2022 - Current	Collaborated in this outreach activity by visiting high schools and delivering the workshop "Integridade em experimentação animal". (https://cienciaetal.i3s.up.pt/?page_id=2997) Workshop	Ciência et al - Programa educativo do i3S Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2015 - Current	Mostra da UP: to represent the IBMC and now i3S in the outreach activity of the University of Porto to reach the general public and future researchers and to disclose the research done in the institute. Exhibition	Mostra da UP Universidade do Porto, Portugal Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2024/01/30 - 2024/01/31	Workshop Formação de Formadores LAS/ "Workshop to train the trainer in Laboratory Animal Science Workshop	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2020 - 2021	Correspondent in the program "Cartas com Ciência" which promotes communication between scientists and students from disadvantaged socioeconomic contexts by exchanging letters. (https://en.cartascomciencia.org/)	Cartas com Ciência, Portugal
2020/02/06 - 2020/02/07	Workshop "Good practices in doctoral supervision" by the Group of Trainers for the Professionalization of the Doctoral Supervision at University Rovira i Virgili Workshop	Good practices in doctoral supervision Universidade do Porto, Portugal Universitat Rovira i Virgili, Spain
2019/03 - 2019/03	Introduction to data analysis and image processing with MATLAB- 5th Edition Workshop	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2017/10/09 - 2017/10/11	Course in Experimental design and analysis of data for research with animals Other	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2017/03 - 2017/03	Workshop in Anesthesia and Euthanasia of Zebrafish and Medaka Workshop	Anesthesia and Euthanasia of Zebrafish and Medaka Karlsruher Institut für Technologie Fakultät für Chemie und Biowissenschaften, Germany
2017/03 - 2017/03	Grant Writing and Reviewing workshop Workshop	AWRN Grant Writing and Reviewing workshop Animal Welfare Research Network (AWRN), United Kingdom
2016/04 - 2016/04	Workshop: addressing the needs for refinement in laboratory fish Workshop	Addressing the needs for refinement in laboratory fish National Centre for the Replacement Refinement and Reduction of Animals in Research, United Kingdom Centre for Environment Fisheries and Aquaculture Science, United Kingdom
2016 - 2016	Noite Europeia dos Investigadores: outreach activity as a researcher talking about my research to the general public, the impact on society and to stimulate interest in research careers, especially amongst young people. Other	Noite Europeia dos Investigadores EU Framework Programme for Research and Innovation Marie Sklodowska-Curie Actions, Belgium Universidade do Porto, Portugal
2012/02 - 2012/02	Workshop on Mice and Rat Colony Management Workshop	Mice and Rat Colony Management Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2010/03 - 2010/03	Memory formation and memory loss Workshop	Memory formation and memory loss Universidad Autónoma de Madrid Facultad de Medicina, Spain
2010/03 - 2010/03	Euron workshop “Drugs and the brain: an update in psychopharmacology” Workshop	Drugs and the brain: an update in psychopharmacology Universidade do Minho, Portugal Universiteit Maastricht, Netherlands
2009/08/24 - 2009/08/29	2nd CNCR Behavior Course (1.8 credits according to ECTS)	Volksuniversiteit Amsterdam, Netherlands
2009/02 - 2009/02	Workshop of Scientific Writing to Senior Researcher Workshop	Workshop of Scientific Writing to Senior Researcher Universidade do Porto, Portugal
2008/03 - 2008/03	Course Fish in Experimental Research Workshop	Fish in Experimental Research Instituto de Medicina Molecular, Portugal

Jury of academic degree

	Topic Role	Candidate name (Type of degree) Institution / Organization
2024/04/11	Chronic Application Of Low Doses Of Ketamine In The Drinking Water Of Mice: Renal Effects And On Behavioural Responses (Thesis) Main arguer	Cláudia Alexandra Soares Rocha (Master) Universidade de Trás-os-Montes e Alto Douro, Portugal
2023/12/15	"Efeitos da presença de visitantes do zoo no comportamento de Pinguins de Humboldt (Spheniscus humboldti) em cativeiro no zoo Santo Inácio, Portugal" (Thesis) Main arguer	Ana Sofia Barros Ferreira Leite Nunes (Master) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2023/09/14	"Efeito do Enriquecimento no polvo-comum (Octopus vulgaris)" (Thesis) Main arguer	Manuel Maria F. de Q. V. e Pinto (Other) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2023/07/31	"Efeito do enriquecimento ambiental no comportamento do Urso Pardo ("Ursus arctos") do Zoo da Maia " (Thesis) Main arguer	Rita Maria Rodrigues Alves Gomes (Other) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2023/07/31	"A influência de enriquecimento ambiental no comportamento da tartaruga-nariz-de-porco (Carettochelys insculpta) residente no Zoo da Maia" (Thesis) Main arguer	Mafalda Sofia Azevedo Veloso (Other) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2021	Brain Expression of the DAB1 Repeat Region causing Spinocerebellar Ataxia Type 37 (Thesis) Main arguer	Catarina Silva Viegas (Master) Universidade do Porto Faculdade de Medicina, Portugal

Ad Hoc journal article review

	Journal title (ISSN)	Publisher
2024 - Current	Fishes	Mdpi
2023 - Current	Communications Biology (2399-3642)
2022 - Current	Veterinary Anaesthesia and Analgesia (1467-2987)
2022 - Current	Toxics (2305-6304)
2022 - Current	Journal of marine science and engineering (2077-1312)
2021 - Current	Journal of Applied Animal Research
2021 - Current	Experimental results
2021 - Current	Heliyon
2021 - Current	Biology
2020 - Current	Aquaculture (0044-8486)
2020 - Current	Pharmacology, Biochemistry and Behavior
2020 - Current	Water
2020 - Current	Aquatic Toxicology
2020 - Current	Biomolecules
2019 - Current	Behavioural Brain Research (0166-4328) (Reviewer)	Elsevier
2019 - Current	Environmental Pollution (0269-7491) (Reviewer)	Elsevier
2018 - Current	Journal of Visualized Experiments (1940-087X) (Reviewer)	MyJoVE Corporation
2018 - Current	Regulatory Toxicology and Pharmacology (1096-0295) (Reviewer)	Elsevier
2017 - Current	Zebrafish (1557-8542) (Reviewer)	Mary Ann Liebert
2017 - Current	PLoS ONE (1932-6203) (Reviewer)	Public Library of Science
2014 - Current	Lab Animal (1548-4475) (Reviewer)	Springer Science and Business Media LLC
2013 - Current	Neurobiology of Learning and Memory (1095-9564) (Reviewer)	Elsevier
2013 - Current	Journal of the American Association for Laboratory Animal Science (1559-6109) (Reviewer)	American Association for Laboratory Animal Science
2013 - Current	Drug and Alcohol Dependence (0376-8716) (Reviewer)	Elsevier
2012 - Current	Laboratory Animals (1758-1117) (Reviewer)	SAGE Publications
2009 - Current	Physiology & Behavior (0031-9384) (Reviewer)	Elsevier

Association member

	Society Organization name	Role
2018 - Current	Zebrafish Husbandry Association: International, US	Member
2016 - Current	EuFishBioMed: Eggenstein-Leopoldshafen, DE	Member
2008 - Current	Sociedade Portuguesa de Animais de Laboratório (SPCAL)	Member

Conference scientific committee

	Conference name	Conference host
2020/10 - Current	3rd PhDay \| Back to the Future	i3S – Instituto de Investigação e Inovação em Saúde in Porto, Portugal
2016/02 - 2017/02	6th annual Laboratory Animal Science (LAS) virtual conference	Labroots

Consulting

	Activity description	Institution / Organization
2022/12 - 2023/01	Invitation to evaluate the analgesia procedures of a project involving zebrafish to be sent to the Swiss authority in charge of animal experimentation (the veterinarian of canton Basel-Stadt) for permission to perform experiments.	Friedrich Miescher Institute for Biomedical Research, Switzerland

Course / Discipline taught

	Academic session	Degree Subject (Type)	Institution / Organization
2017 - Current	Ethological analysis of behaviour	Neurobiologia (Mestrado integrado)	Universidade do Porto Faculdade de Medicina, Portugal
2016 - Current	Zebrafish procedures - practical lessons	Course in Laboratory Animal Science	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2012 - Current	Ethological analysis of behaviour	Neurociências (Doutoramento)	Universidade do Porto Faculdade de Medicina, Portugal
2008 - Current	Refinement of experimental procedures, humane endpoints and euthanasia	Course in Laboratory Animal Science (Outros)	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2024/02/23 - 2024/03/01	Training the trainer in Animal Welfare in Aquaculture and Public Aquariums module to the WP of the ERASMUS project: AFISHE- Development of aquaculture and fisheries education for green deal in Armenia and Ukraine: from education to ecology		Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2021/01 - 2022/01	"Métodos Humanos de Occisão"	Curso de Ciências de Animais de Laboratório (Outros)	Instituto Politécnico de Leiria, Portugal
2016 - 2019	Ethics, 3 R’s and scientific integrity	Curso de Ciências de Animais Aquáticos de Laboratório (Cal-Aqua) (Outros)	Universidade do Porto Centro Interdisciplinar de Investigação Marinha e Ambiental, Portugal
2016 - 2019	Zebrafish biology, housing and maintenance	Laboratory animal science course (Outros)	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2008 - 2019	Handling / Minor procedures in rodents - practical classes	Course in Laboratory Animal Science	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2016/11 - 2016/11	Comportamento e Bem-estar em animais de laboratório - Behaviour and welfare in laboratory animals (invited lecturer)	Medicina Veterinária (Licenciatura)	Escola Universitária Vasco da Gama, Portugal
2011 - 2011	Introduction to scientific research (invited teacher)	Ciências Veterinárias (Doutoramento)	Universidade de Trás-os-Montes e Alto Douro Escola de Ciências Agrárias e Veterinárias, Portugal

Evaluation committee

	Activity description Role	Institution / Organization	Funding entity
2024 - 2024	Project evaluator in the Swiss 3RCC Refinement Review Panel Evaluator	Swiss 3R competence center, Switzerland

Mentoring / Tutoring

	Topic	Student name
2022/01 - Current		Part of the UFAW Mentoring Scheme, UK
2021 - 2022	Laboratory Animal Science course	Inês Caetano
2017 - 2017	Laboratory Animal Science course	Gabriela Munhoz Morello
2014 - 2014	Laboratory Animal Science course	Jorge Miguel Ferreira
2014 - 2014	Laboratory Animal Science course	Margarida Pinto Monteiro
2013 - 2013	Acute administration of ketamine and its anesthetic combinations: influence on anxiety in adult rats.	Sofia Leal Santos
2012 - 2012	Laboratory Animal Science course	Silvana Guedes
2007 - 2008	Effects of medetomidine/ketamine anaesthesia combination in mice at brain level	Patrícia do Céu Ribeiro

Distinctions

Award

2023	AE2023 (Aquaculture Europe) Best Student poster - co-author and supervisor European Aquaculture Society, Belgium
2015	Third best poster in the I Iberian Congress for Laboratory Animal Science Sociedad Española para las Ciencias del Animal de Laboratorio, Spain
2010	Travel grant - FLAD C&T Papers 2010 to present oral communications in Conferences in the USA Fundação Luso-Americana, Portugal

Other distinction

2015	UFAW Animal Welfare Student Scholarship - supervisor Universities Federation for Animal Welfare, United Kingdom
2014	Individual Post-doc fellowship grant Fundação para a Ciência e a Tecnologia, I.P., Portugal
2008	Individual PhD scholarship grant Fundação para a Ciência e a Tecnologia, I.P., Portugal
2004	Prize for the Best Student of Faculdade de Ciência e Tecnologia de Universidade de Coimbra (2003/2004) Universidade de Coimbra, Portugal