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My name is João Gabriel Silva. I'm currently a PhD Fellow at Doctoral Program of Experimental Biology and Biomedicine at the Institute of Interdisciplinary Research at University of Coimbra, supported by a grant from Fundação para a Ciência e Tecnologia. My project focuses on working with a zebrafish model for hepatic fluxes in diet-induced NAFLD context, hosted by Center of Neurosciences and Cell Biology at University of Coimbra, CICECO at University of Aveiro, and CIVG at Escola Universitária Vasco da Gama in Coimbra. I have a BSc in Biology in 2019 (final classification of 17) and a MSc in Cellular and Molecular Biology in 2021 (final classification of 19), both by University of Coimbra. My thesis was titled "The Effects Of High Fat Feeding On The Metabolomics Of Heart, Kidney And Skeletal Muscle In C57BL6J Mice As Analysed By 1H NMR", supervised by Doctor Ludgero Tavares, Professor Ana Gil and Professor Rui Carvalho, and an article as a main author is being prepared from this work. Currently I have 4 articles published in international journals, accumulating 28 citations in the past 3 years, and 4 participations in a international and national conferences posters, one awarded the 3rd Best Poster and another with an Honourable Mention. Additionally, I have been awarded with the prize "3% Best Students" in 2017 and" Merit Board" in 2018, both at University of Coimbra, as well as multiple honour awards in basic and high-school. During my time at University of Coimbra, I was a Collaborator (2016-2018) and then the Coordinator (2018-2020) of Scientific Formation and Professional Goals at the Nuclei of Biology Students of the Academic Association of Coimbra, organizing several scientific events within the Student Association and the Research Centres of the Universities. Furthermore, I was also a participant in different workshops, courses, meetings and conferences, as well as a volunteer in different organizations. My current research interests focus on Metabolism, Metabolomics and Big Data tools to better understand and characterise diseases such as the Metabolic Syndrome, Non-alcoholic Fatty Liver Disease and Diabetes, employing Nuclear Magnetic Resonance (NMR), Stable Isotope Tracers, and Multivariate Analyse Tools. During my (still short) academic experience I had different learning periods: a summer internship in Escola Superior Agrária, working in horses reproduction; a long term internship in the Metabolic Control Center in the Center of Neurosciences and Cell Biology in Coimbra, studying fish and mice metabolism; and more recently, I secured a position as a fellowship researcher at the Metabolomics Lab in CICECO-Aveiro, in the project "The role of extrahepatic fructose metabolism in the development of non-alcoholic fatty liver disease", acquiring and analysing NMR data from mice with different energetic diets. From my past research work, I have experience techniques such as NMR Spectroscopy, stable isotope labelling, HPLC and sample processing in extraction and purification of mice tissues (tissue homogenizers, liquid nitrogen grinding, freeze-drying, MTBE and Folch extractions, solid phase extractions for triglycerides). Additionally, I had contact with statistical tools in Multivariate Analysis (PCA, PLS-DA and OPLS-DA), and well as software such as Python, R, GraphPad Prism, Excel, MATLAB, SIMCA, and NMR tools (TopSpin, Amix and Chenomx).
Identification

Personal identification

Full name
João Gabriel Silva

Citation names

  • Silva, João Gabriel
  • Silva, J G

Author identifiers

Ciência ID
B61E-1EFB-F5A0
ORCID iD
0000-0002-1150-1893
Google Scholar ID
https://scholar.google.com/citations?user=mfk0C1AAAAAJ&hl=en
Researcher Id
ABG-1901-2021
Scopus Author Id
57218329674

Addresses

  • CNC University of Coimbra Rua Larga Faculty of Medicine, Pólo I, 1st floor, 3004-504, Coimbra, Coimbra, Portugal (Professional)
  • Biocant Park - UC Biotech, Parque Tecnológico de Cantanhede, Núcleo 04, Lote 3, 3060-197, Cantanhede, Cantanhede, Portugal (Professional)
  • Complexo de Laboratórios Tecnológicos Campus Universitário de Santiago, 3810-193, Aveiro, Aveiro, Portugal (Professional)

Websites

Knowledge fields

  • Natural sciences - Biological Sciences - Molecular Biology
  • Natural sciences - Biological Sciences - Cell Biology
  • Natural sciences - Biological Sciences - Biochemistry
  • Medical and Health Sciences - Clinical Medicine - Endocrinology and Metabolism
  • Medical and Health Sciences - Health Sciences

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese (Mother tongue)
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Spanish; Castilian Intermediate (B1) Upper intermediate (B2) Intermediate (B1) Upper intermediate (B2)
Education
Degree Classification
2022/09 - 2026
Ongoing
Doctoral Program in Experimental Biology and Biomedicine (Doutoramento)
Universidade de Coimbra Instituto de Investigação Interdisciplinar, Portugal
2023/09/11 - 2023/09/22
Concluded
Laboratory Animal Science Course - FELASA Accredited Course 020/08F (Outros)
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2023 - 2023
Concluded
Advanced Course Proteomics Approaches in Life Sciences (Curso de doutoramento (conclusão de unidades curriculares))
Universidade de Coimbra Instituto de Investigação Interdisciplinar, Portugal
19
2023 - 2023
Concluded
Advanced Course Neuroscience - Bioinformatics and Computational Biology (Curso de doutoramento (conclusão de unidades curriculares))
Universidade de Coimbra Instituto de Investigação Interdisciplinar, Portugal
17
2023 - 2023
Concluded
Advanced Course Principles and Practices in Drug Development (Curso de doutoramento (conclusão de unidades curriculares))
Universidade de Coimbra Instituto de Investigação Interdisciplinar, Portugal
19
2023 - 2023
Concluded
Advanced Course Ageing (Curso de doutoramento (conclusão de unidades curriculares))
Universidade de Coimbra Instituto de Investigação Interdisciplinar, Portugal
17
2023 - 2023
Concluded
Advanced Course ONE HEALTH: A Metabolic Approach from the Cell to Society (Curso de doutoramento (conclusão de unidades curriculares))
Universidade de Coimbra Instituto de Investigação Interdisciplinar, Portugal
18
2022 - 2022
Concluded
Advanced Course Connecting Researchers with the Society and Industry (Curso de mestrado (conclusão do curso de especialização))
Universidade de Coimbra Instituto de Investigação Interdisciplinar, Portugal
18
2022 - 2022
Concluded
Advanced Course Core Technologies (Curso de doutoramento (conclusão de unidades curriculares))
Universidade de Coimbra Instituto de Investigação Interdisciplinar, Portugal
18
2022 - 2022
Concluded
Advanced Course Experimental Science Design (Curso de doutoramento (conclusão de unidades curriculares))
Universidade de Coimbra Instituto de Investigação Interdisciplinar, Portugal
19
2019/09 - 2021/09
Concluded
Cellular and Molecular Biology (Mestrado)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
"The effects of high fat feeding on the metabolomics of heart, kidney and skeletal muscle in C57BL6J mice as analysed by 1H NMR" (THESIS/DISSERTATION)
19
2016/09 - 2019/06
Concluded
Biology (Licenciatura)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
17
2018/07 - 2019/03
Concluded
Quantification of adenine nucleotides by HPLC (Outros)
Universidade de Coimbra, Portugal
2015/06/29 - 2015/07/03
Concluded
Summer Internship - Ciência Viva no Laboratório - Ocupação Científica de Jovens nas Férias (Outros)
Instituto Politécnico de Coimbra Escola Superior Agrária de Coimbra, Portugal
Affiliation

Science

Category
Host institution
Employer
2022/09 - Current Researcher (Research) Fundação para a Ciência e a Tecnologia, Portugal
Universidade de Coimbra Centro de Inovação em Biomedicina e Biotecnologia, Portugal
(...)
2020/09 - 2021/06 Researcher (Research) Universidade de Aveiro CICECO, Portugal
Universidade de Aveiro Departamento de Química, Portugal
(...)
2018/07 - 2020/08 Research Trainee (Research) Universidade de Coimbra, Portugal
Universidade de Coimbra, Portugal
(...)
Projects

Grant

Designation Funders
2020/09/01 - 2021/01/01 VISCERAL: The role of extrahepatic fructose metabolism in the development of non-alcoholic fatty liver disease
POCI-01-0145-FEDER-028147
Research Fellow
Universidade de Aveiro CICECO, Portugal

Universidade de Coimbra Centro de Inovação em Biomedicina e Biotecnologia, Portugal

Centro de Neurociências e Biologia Celular, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
Outputs

Publications

Conference abstract
  1. Silva, João Gabriel; Ludgero C. Tavares; Belew, Getachew Debas; Gil, Ana; Jones, John. Corresponding author: Jones, John. "Beyond the liver: Impact of high-fat-induced MASLD in the lipidomic profiles of mice extrahepatic tissues". Paper presented in 58th Annual Scientific Meeting of the European Society for Clinical Investigation, Barcelona, 2024.
    Accepted • https://doi.org/10.1111/eci.14220
  2. Silva, João Gabriel; Ludgero, L C; Belew, Getachew Debas; Gil, Ana; Jones, John. Corresponding author: Jones, John. "Indo além do Fígado: Impacto de dFGNA induzida por dieta no perfil lipídico de tecidos extra-hepáticos de murganhos". Paper presented in 20º Congresso Português de Diabetes, Vilamoura, 2024.
    Published
  3. Silva, João Gabriel; Tavares, L C; Belew, Getachew Debas; Carvalho, R A; Gil, Ana; Jones, John. Corresponding author: Jones, John. "Tecidos extra-hepáticos e dFGNA induzida pela dieta: uma nova perspectiva metabolómica". Paper presented in 19th Portuguese Diabetes Congress, Vilamoura, 2023.
    Published
  4. Belew, G D; Silva, João Gabriel; Rito, J; Viegas, I; Teixeira, J; Oliveira, P J; Tavares, L C; Jones, J G. "13C NMR of hepatic triglyceride from mice fed fructose plus [U-13C]glucose informs the contribution of glucose to de novo lipogenesis and elongation of specific fatty acid classes". Paper presented in 7th EASD NAFLD Study Group Meeting, Lisbon, 2019.
    Published
  5. Tavares, L C; Belew, G D; Silva, João Gabriel; Rito, J; Viegas, I; Teixeira, J; Oliveira, P J; Jones, J G. "The distribution of saturated, monounsaturated and polyunsaturated fatty acids in the sn2 and sn1,3 positions of hepatic triglyceride is not homogenous". Paper presented in 7th EASD NAFLD Study Group Meeting, Lisbon, 2019.
    Published
Conference poster
  1. Silva, João Gabriel; Tavares, L C; Belew, Getachew Debas; Gil, Ana; Jones, John. "Exploring the lipidomic profiles of mice extrahepatic tissues in high-fat-induced NAFLD using 1H-NMR". Paper presented in IV Metabolism Ageing and Disease Meeting, 2024.
  2. Silva, João Gabriel; Ludgero, L C; Belew, Getachew Debas; Carvalho, RA; Carvalho, R; Carvalho Rui; Gil, Ana; Jones, John. Corresponding author: Silva, João Gabriel. "Tecidos extra-hepáticos e dFGNA induzida pela dieta: uma nova perspetiva através de metabolómica". Paper presented in 19º Congresso Português de Diabetes, 2023.
  3. Silva, João Gabriel; Ludgero, L C; Carvalho, RA; Carvalho, R; Carvalho Rui; Gil, Ana; Jones, John. Corresponding author: Silva, João Gabriel. "Extrahepatic Tissue And Diet-induced NAFLD: A New Insight Through Metabolomics". Paper presented in BEBDay 2023, 2023.
  4. Silva, João Gabriel; Tavares, L C; Gil, Ana M.; Jones, John. Corresponding author: Silva, João Gabriel. "Non-Alcoholic Fatty Liver Disease and Western Diet: A Tale of the Effects in the Metabolomics of the Heart". Paper presented in VI meet.Lab 2022, 2022.
  5. Palma, Mariana; Tavares, L C; Rito, Joao; Henriques, Luís; Silva, João Gabriel; Rema, P; Ozório, R.O.A.; et al. Corresponding author: Palma, Mariana. "Effects of Dietary Glycerol Supplementation in Liver And Muscle Metabolome: Comparative Study In European Seabass And Rainbow Trout". Paper presented in Aquaculture Europe 2019 – Berlin, Germany, 2019.
Journal article
  1. Ivan Viegas; Giada Di Nunzio; Getachew D. Belew; Alejandra N. Torres; João G. Silva; Luis Perpétuo; Cristina Barosa; Ludgero C. Tavares; John G. Jones. "Integration of Liver Glycogen and Triglyceride NMR Isotopomer Analyses Provides a Comprehensive Coverage of Hepatic Glucose and Fructose Metabolism". Metabolites (2022): https://doi.org/10.3390/metabo12111142.
    10.3390/metabo12111142
  2. DiNunzio, Giada; Belew, Getachew D.; Torres, Alejandra N.; Silva, João Gabriel; Silva, Luis P.; Barosa, Cristina; Tavares, Ludgero; Jones, John G.. "Determining the contribution of a high-fructose corn syrup formulation to hepatic glycogen synthesis during ad-libitum feeding in mice". Scientific Reports 10 1 (2020): http://dx.doi.org/10.1038/s41598-020-69820-3.
    10.1038/s41598-020-69820-3
  3. Belew, Getachew D.; Di Nunzio, Giada; Tavares, Ludgero; Silva, Joao G.; Torres, Alejandra N.; Jones, John G.. "Estimating pentose phosphate pathway activity from the analysis of hepatic glycogen 13 C-isotopomers derived from [U- 13 C]fructose and [U- 13 C]glucose". Magnetic Resonance in Medicine 84 5 (2020): 2765-2771. http://dx.doi.org/10.1002/mrm.28286.
    10.1002/mrm.28286
  4. Palma, Mariana; Tavares, Ludgero; Rito, João; Henriques, Luís; Silva, João; Ozório, Rodrigo; Pardal, Miguel; Magnoni, Leonardo; Viegas, Ivan. "Metabolic Effects of Dietary Glycerol Supplementation in Muscle and Liver of European Seabass and Rainbow Trout by 1H NMR Metabolomics". Metabolites 9 10 (2019): 202. http://dx.doi.org/10.3390/metabo9100202.
    10.3390/metabo9100202
Thesis / Dissertation
  1. "The effects of high fat feeding on the metabolomics of heart, kidney and skeletal muscle in C57BL6J mice as analysed by 1H NMR". Master, Universidade de Coimbra, 2021. https://eg.uc.pt/handle/10316/96156.
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2024/06/06 Beyond the liver: Impact of high-fat-induced MASLD in the lipidomic profiles of mice extrahepatic tissues 58th Annual Scientific Meeting of the European Society for Clinical Investigation
(Barcelona , Spain)
2023/05/08 The effects of high fat feeding on the metabolomics of heart, kidney and skeletal muscle in C57BL6J mice as analysed by 1H NMR "Methodologies in Research and Development", class part of the Integrated Masters in Veterinarian Medicine at Escola Universitária Vasco da Gama
Escola Universitária Vasco da Gama (Coimbra, Portugal)

Event organisation

Event name
Type of event (Role)
Institution / Organization
2023/09/29 - 2023/09/29 Researcher at booth "68. Poder Sustentável e Inclusivo - As Nossas Mitocôndrias." (2023/09/29 - 2023/09/29)
Other (Other)
2019 - 2019 III meet.Lab - Encontro Nacional de Estudantes de Laboratório (2019 - 2019)
Meeting (Member of the Organising Committee)
Universidade de Coimbra, Portugal

Núcleo de Estudantes de Biologia da Associação Académica de Coimbra, Portugal
2018 - 2018 II meet.Lab - Encontro Nacional de Estudantes de Laboratório (2018 - 2018)
Meeting (Member of the Organising Committee)
Universidade de Coimbra, Portugal

Núcleo de Estudantes de Biologia da Associação Académica de Coimbra, Portugal

Event participation

Activity description
Type of event
Event name
Institution / Organization
2023/03/16 - 2023/03/18 Portuguese National Congress on Diabetology for Fundamental, Applied and Medical Research.
Congress
19º Congresso Português de Diabetes
Sociedade Portuguesa de Diabetologia, Portugal
2023/02/07 - 2023/02/08 Conference organized by senior PhD Students of the Doctoral Program in Experimental Biology and Biomedicine. Objetive is for younger and senior students to present their works and attend talks of national and international speakers.
Conference
BEBDay 2023
Universidade de Coimbra Instituto de Investigação Interdisciplinar, Portugal
2022/11/08 - 2022/12/06 Training programme in Bioentrepreneurship, BioSTART, from the 8th of November to the 6th of December 2022, with the total duration of 16 hours
Other
BioSTART (Bioentrepreneurship Course)
Centro de Neurociências e Biologia Celular, Portugal

Universidade de Coimbra, Portugal

Biocant Associação de Transferência de Tecnologia, Portugal
2022/11/23 - 2022/11/28 2 day workshop in coding language R. Organized by Núcleo de Estudantes de Biologia
Workshop
R Workshop, Part I and II
Núcleo de Estudantes de Biologia da Associação Académica de Coimbra, Portugal
2022/11/10 - 2022/11/10 Identifying skills and strengths - Chaperone's Career Development Workshop
Workshop
Identifying skills and strengths - Chaperone's Career Development Workshop
Chaperone, Portugal
2022/10/19 - 2022/10/19 Data Analysis | Python - European Code Week (Webinar)
Workshop
Data Analysis | Python
2022/09/28 - 2022/09/28 Gibco - 60 Years loving your cells - Group of Seminars
Seminar
Gibco - 60 Years loving your cells
Thermo Fisher Scientific Inc Pittsburgh, United States
2021/09/29 - 2021/09/29 Intermediate Benchtop NMR Spectroscopy (Webinar)
Seminar
Intermediate Benchtop NMR Spectroscopy
Oxford Instruments Magnetic Ressonance, United Kingdom
2021 - 2021 9th IberoAmerican NMR meeting
Congress
Grupo Especializado de Resonancia Magnética Nuclear, Spain
2019 - 2019 Fish Metabolism - Applications to Aquaculture (Flash Course)
Conference
Universidade de Coimbra Departamento de Ciências da Vida, Portugal

Centro de Ecologia Funcional, Portugal

Centro de Neurociências e Biologia Celular, Portugal
2019 - 2019 How to write a great research paper and get published - Researcher Academy On Campus
Seminar
Elsevier Inc, United States

Universidade de Coimbra, Portugal
2018 - 2018 III Meet.Eco Coimbra - Encontro Nacional de Estudantes de Ecologia
Meeting
Universidade de Coimbra, Portugal

Núcleo de Estudantes de Biologia da Associação Académica de Coimbra, Portugal
2017 - 2017 XX Encontro Nacional de Estudantes de Biologia
Meeting
Universidade de Aveiro, Portugal
2017 - 2017 I meet.Lab - Encontro Nacional de Estudantes de Laboratório
Meeting
Universidade de Coimbra, Portugal

Núcleo de Estudantes de Biologia da Associação Académica de Coimbra, Portugal
2017 - 2017 Excel Workshop Level I and II
Workshop
Universidade de Coimbra, Portugal

Núcleo de Estudantes de Biologia da Associação Académica de Coimbra, Portugal
2017 - 2017 Computer Building Workshop
Workshop
Universidade de Coimbra, Portugal

Núcleo De Engenharia Eletrotrotécnica e de Computadores da AssociaçãoAcadémica de Coimbra, Portugal

Association member

Society Organization name Role
2018 - 2020 Núcleo de Estudantes de Biologia da Associação Académica de Coimbra Coordinator of Scientific Formation and Professional Goals
2017 - 2018 AFS Training Novos Voluntários Volunteer
2016 - 2018 Núcleo de Estudantes de Biologia da Associação Académica de Coimbra Collaborator of Scientific Formation and Professional Goals
2008 - 2018 Liga Portuguesa Contra o Cancro and Banco Alimentar Contra a Fome while in the Scouts Volunteer
Distinctions

Award

2023 Honourable Mention Poster - Poster in Fundamental Research
Sociedade Portuguesa de Diabetologia, Portugal
2022 3rd Best Poster Presentation at VI meet.Lab Coimbra
Núcleo de Estudantes de Biologia da Associação Académica de Coimbra, Portugal
2018 Merit Board at University of Coimbra
Universidade de Coimbra, Portugal
2017 3% Best Students at University of Coimbra
Universidade de Coimbra, Portugal
2016 Honour Student (All Years from 2008/2009 to 2015/2016)
Instituto Educativo de Souselas, Portugal

Other distinction

2022 CIBB-CNC - FCT PhD Fellowship
Universidade de Coimbra Centro de Inovação em Biomedicina e Biotecnologia, Portugal

Centro de Neurociências e Biologia Celular, Portugal

Fundação para a Ciência e a Tecnologia, Portugal