Sandra Sousa (BC16-DBBF-D5C2)

Curriculum Vitae

Sandra Sousa

Sandra Sousa is a microbiologist with deep interest in Host-pathogen interactions. She obtained a PhD in the laboratory of Pascale Cossart (Institut Pasteur, Paris) studying the bacterial mechanisms triggering key host signaling pathways to facilitate bacterial invasion of mammalian cells. S. Sousa has been focusing her research on the understanding of mechanisms of host usurpation by relevant human pathogens. She has extensive expertise in host cytoskeleton remodeling induced in response to bacterial infection and to bacterial plasma membrane damaging factors, such as secreted pore-forming toxins. S. Sousa leads the group of Cell Biology of Bacterial Infections at i3S since June 2019. S. Sousa published more than 40 articles in high impact peer-reviewed journals (1900 citations, h-index 20), completed the supervision of 4 PhD thesis and 8 MSc dissertations, and secured funds to her research. Her current research focus on intracellular responses to plasma membrane damage caused by pore-forming toxins, which are secreted by relevant human pathogens and play key roles in infection. In particular, she concentrates on plasma membrane and actomyosin remodeling, ER disruption and calcium signaling upon damage induced by different pore-forming toxins.

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Identification

Personal identification

Full name: Sandra Sousa

Citation names

Sousa, Sandra

Author identifiers

Ciência ID: BC16-DBBF-D5C2
ORCID iD: 0000-0001-8578-0461

Email addresses

srsousa@ibmc.up.pt (Professional)
srsousa@i3s.up.pt (Professional)

Addresses

i3S, IBMC. Rua Alfredo Allen, 208, 4200-135, Porto, Porto, Portugal (Professional)

Websites

https://www.i3s.up.pt/research-group.php?groupid=137 (Professional)

Knowledge fields

Natural sciences - Biological Sciences - Microbiology

Languages

Language	Speaking	Reading	Writing	Listening	Peer-review
Portuguese (Mother tongue)
English	Advanced (C1)	Advanced (C1)	Advanced (C1)	Advanced (C1)	Advanced (C1)
French	Proficiency (C2)	Proficiency (C2)	Proficiency (C2)	Proficiency (C2)	Proficiency (C2)
Spanish; Castilian	Intermediate (B1)	Upper intermediate (B2)	Elementary (A2)	Intermediate (B1)	Intermediate (B1)

Education

	Degree	Classification
2000/11/02 - 2004/11/17 Concluded	Microbiology and Virology (Doctorat) Université Paris VII Denis Diderot UFR Sciences du Vivant, France Institut Pasteur, France "Mécanismes d'entrée de Listeria monocytogenes dans les céllules non-phagocytaires: Etude des facteurs cellulaires en aval de l'interaction InlA/E-cadhérine" (THESIS/DISSERTATION)
1999/09 - 2000/09 Concluded	General Microbiology (DEA) Université Paris VII Denis Diderot UFR Sciences du Vivant, France Institut Pasteur, France Ecole Normale Supérieure Département de Biologie, France
1993/09/05 - 1998/07/30 Concluded	Microbiologia (Licenciatura) Universidade Católica Portuguesa Escola Superior de Biotecnologia, Portugal	17

Affiliation

Science

	Category Host institution	Employer
2023/03/01 - Current	Principal Investigator (Research)	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2019/09 - 2023/02/28	Auxiliary Researcher (Research)	Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal (...)
2019/01/01 - 2019/08/31	Auxiliary Researcher (Research)	Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal (...)
2014/01/01 - 2018/12/31	Auxiliary Researcher (Research)	Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
	Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal (...)
2009/06/01 - 2013/12/31	Auxiliary Researcher (Research)	Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
	Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2006/01/01 - 2009/05/31	Postdoc (Research)	Fundação para a Ciência e a Tecnologia, Portugal
	Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2005/01/01 - 2005/12/31	Postdoc (Research)	Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
	Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2000/11/01 - 2004/10/31	Research Trainee (Research)	Fundação para a Ciência e a Tecnologia, Portugal
	Institut Pasteur, France
1999/09/01 - 2000/08/31	Research Trainee (Research)	Fundação para a Ciência e a Tecnologia, Portugal
	Ecole Normale Supérieure Département de Biologie, France

Projects

Grant

	Designation	Funders
2018/08 - 2022/07	Enlighten host plasma membrane repair mechanisms upon bacterial pore-forming toxins attack POCI-01-0145-FEDER-030863 Principal investigator Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal	Ministério da Ciência Tecnologia e Ensino Superior Ongoing
2018/07 - 2022/06	Cell Wall glycosylation: an original target for innovative antimicrobial strategies against Gram-positive pathogens POCI-01-0145-FEDER-0320 Researcher Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal	Fundo Regional para a Ciência e Tecnologia Ongoing
2016 - 2019	Structured Program on Bioengineering Therapies for Infectious Diseases and Tissue Regeneration NORTE-01-0145-FEDER-000012 Researcher Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal	Fundo Regional para a Ciência e Tecnologia Concluded
2014/09 - 2017/08	Subversive pro- and anti-inflammation trade-offs promote infection by Listeria monocytogenes Provided by PTCRIS: 137216 Researcher Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2014/06 - 2016/12	Novel interfaces during host responses to pore forming toxins: crosstalk between ER chaperones and cytoskeletal proteins ESCMID Research Grant 2014 Principal investigator Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal	European Society of Clinical Microbiology and Infectious Diseases Concluded
2011/04 - 2015/09	Unraveling the interplay between Listeria monocytogenes infection and the host cell cycle Provided by PTCRIS: 111215 111215 Principal investigator Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal	Fundação para a Ciência e a Tecnologia, I.P. Concluded
2013 - 2015	Host Pathogen Interactions NORTE-01-0124-FEDER-00002 Researcher Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal	Fundo Regional para a Ciência e Tecnologia Concluded
2011/08 - 2014/08	Characterization of two new Listeria monocytogenes virulence genes up-regulated during in vivo infection: role of cadmium efflux system and teichoic acid decoration in Listeria pathogenicity 111581 Provided by PTCRIS: 111581 Researcher	Fundação para a Ciência e a Tecnologia, I.P. Concluded
2011/03 - 2014/08	LISTRESS. Analysis of the cellular mechanisms underlying the early response of the host to stress induced by Listeria infection: Teichoic acid modifications, surface protein anchoring and virulence. Provided by PTCRIS: 116472 116472 Researcher	Fundação para a Ciência e a Tecnologia, I.P. Concluded
2010/05 - 2014/07	New host factors hijacked by human bacterial pathogens to promote and establish infection: role of tyrosine-phosphorylated Myosin IIA and Cytokeratin 18 100088 Provided by PTCRIS: 100088 Principal investigator Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal	Fundação para a Ciência e a Tecnologia, I.P. Concluded
2007/10 - 2011/09	Host-pathogen interactions: role of Vip-Gp96 complex in the virulence of Listeria monocytogenes Provided by PTCRIS: 65407 65407 Post-doc Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal	Fundação para a Ciência e a Tecnologia, I.P. Concluded
2007/10 - 2011/01	Identification of novel bacterial factors implicated in the virulence of Listeria monocytogenes Provided by PTCRIS: 65406 65406 Post-doc Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal	Fundação para a Ciência e a Tecnologia, I.P. Concluded
2007/02 - 2010/07	SPATELIS - “Spatio-temporal analysis of Listeria-host protein interactions” Provided by PTCRIS: 81993 Post-doc Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2005/04 - 2008/09	Listeria as a model for the analysis of pathogen and host factors that determine the fate of infections Provided by PTCRIS: 60443 60443 Post-doc	Fundação para a Ciência e a Tecnologia, I.P. Concluded

Contract

	Designation	Funders
2019/01/01 - 2019/12/31	Institute for Research and Innovation in Health Sciences UID/BIM/04293/2019 LA/P/0070/2020 10.54499/LA/P/0070/2020 Instituto Nacional de Engenharia Biomédica, Portugal Universidade do Porto, Portugal Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal Universidade do Porto Instituto de Patologia e Imunologia Molecular, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2014/09/01 - 2017/08/31	A subversão do equilíbrio da resposta pro- e anti-inflamatória promove a infecção por Listeria monocytogenes Infect-ERA/0001/2013 Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2011/04/01 - 2014/09/30	Estudo da influência da infecção por Listeria monocytogenes no ciclo celular da célula hospedeira PTDC/BIA-BCM/111215/2009 Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2011/01/01 - 2012/12/31	Projecto Estratégico - LA 2 - 2011-2012 PEst-C/SAU/LA0002/2011 Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal Instituto Nacional de Engenharia Biomédica, Portugal	Fundação para a Ciência e a Tecnologia Concluded

Outputs

Publications

Book chapter	Cabanes, Didier; Sousa, Sandra; Cossart, Pascale. "Listeria Genomics". In Genomics of Foodborne Bacterial Pathogens, edited by Wiedmann, Martin; Zhang, Wei (Eds.), 141. Springer New York, 2011. Published • 10.1007/978-0-387-49376-3_3
Conference poster	Meireles, Diana; Pombinho R.; Lisboa, Johnny; Monteiro, Ricardo; Sousa, Sandra; Morais-Cabral, João; Cabanes, Didier. "Unravelling signals underlying Listeria monocytogenes cadC regulation". Paper presented in European Congress of Clinical Microbiology and Infectious Diseases, 2024. Meireles, Diana; Pombinho R.; Lisboa, Johnny; Monteiro, Ricardo; Sousa, Sandra; Morais-Cabral, João; Cabanes, Didier. "Uncovering the signals behind the expression of the Listeria monocytogenes regulator CadC". Paper presented in 10th Molecular and Cell Biology Symposium, 2024. Pereira, J. M.; Alves, Sara; Xu, Shuying; Cabanes, Didier; Leong, John; Sousa, Sandra. "S100A4 is a master regulator of cytoskeleton remodeling induced by pneumolysin pore formation". Paper presented in Cell Bio 23, 2023. Meireles, Diana; Pombinho, Rita; Sousa, Sandra; Morais-Cabral, João; Cabanes, Didier. Corresponding author: Cabanes, Didier. "Signals controlling the expression of the Listeria monocytogenes virulence regulator CadC". Paper presented in EMBO \| EMBL Symposium: New approaches and concepts in microbiology, 2023. Alves, Ângela; Pombinho R.; Sousa, Sandra; Cabanes, Didier. "PurA, an adenylosuccinate synthetase required for Listeria monocytogenes intracellular lifecycle". Paper presented in EMBO \| EMBL Symposium: New approaches and concepts in microbiology, 2023. Pombinho, Rita; Meireles, Diana; Pombinho, António; Maia, André; Sousa, Sandra; Cabanes, Didier. Corresponding author: Pombinho, Rita. "Automated high-throughput microscopy screen for Listeria monocytogenes factors required for intracellular infection". Paper presented in 32nd ECCMID Congress, 2022. Pereira, J. M.; Alves, Sara; Cabanes, Didier; Sousa, Sandra. "Mechanisms of host resistance to plasma membrane damage induced by pneumolysin attack". Paper presented in ISEV2020, 2020. Monteiro, R.; Meireles, Diana; Cereija, T. B.; Pombinho, António; Morais-Cabral, João; Sousa, Sandra; Cabanes, Didier. "Shaping the activity of Listeria monocytogenes rhamnosyltransferase RmlT as auspicious antivirulence strategy.". Paper presented in i3S annual meeting 2019, 2019.
Journal article	Cláudia Brito; Joana M. Pereira; Francisco S. Mesquita; Didier Cabanes; Sandra Sousa. "Src-Dependent NM2A Tyrosine Phosphorylation Regulates Actomyosin Remodeling". Cells (2023): https://doi.org/10.3390/cells12141871. 10.3390/cells12141871 Sara Alves; Joana M. Pereira; Rupert L. Mayer; Alexandre D. A. Gonçalves; Francis Impens; Didier Cabanes; Sandra Sousa. "Cells Responding to Closely Related Cholesterol-Dependent Cytolysins Release Extracellular Vesicles with a Common Proteomic Content Including Membrane Repair Proteins". Toxins (2022): https://doi.org/10.3390/toxins15010004. 10.3390/toxins15010004 Pereira, Joana M.; Xu, Shuying; Leong, John M.; Sousa, Sandra. "The Yin and Yang of Pneumolysin During Pneumococcal Infection". Frontiers in Immunology 13 (2022): http://dx.doi.org/10.3389/fimmu.2022.878244. Open access • Published • 10.3389/fimmu.2022.878244 Rita Pombinho; Jorge Pinheiro; Mariana Resende; Diana Meireles; Sirpa Jalkanen; Sandra Sousa; Didier Cabanes. "Stabilin-1 plays a protective role against Listeria monocytogenes infection through the regulation of cytokine and chemokine production and immune cell recruitment". Virulence 12 1 (2021): 2088-2103. https://doi.org/10.1080/21505594.2021.1958606. 10.1080/21505594.2021.1958606 Cláudia Brito; Sandra Sousa. "Non-Muscle Myosin 2A (NM2A): Structure, Regulation and Function". Cells (2020): https://doi.org/10.3390/cells9071590. 10.3390/cells9071590 Ana Catarina Costa; Jorge Pinheiro; Sandra A. Reis; Didier Cabanes; Sandra Sousa. "Listeria monocytogenes Interferes with Host Cell Mitosis through Its Virulence Factors InlC and ActA". Toxins (2020): https://doi.org/10.3390/toxins12060411. 10.3390/toxins12060411 Diana Meireles; Rita Pombinho; Filipe Carvalho; Sandra Sousa; Didier Cabanes. "Listeria monocytogenes Wall Teichoic Acid Glycosylation Promotes Surface Anchoring of Virulence Factors, Resistance to Antimicrobial Peptides, and Decreased Susceptibility to Antibiotics". Pathogens (2020): https://www.mdpi.com/2076-0817/9/4/290. 10.3390/pathogens9040290 Pombinho, Rita; Vieira, Ana; Camejo, Ana; Archambaud, Cristel; Cossart, Pascale; Sousa, Sandra; Cabanes, Didier. "Virulence gene repression promotes Listeria monocytogenes systemic infection". Gut Microbes (2020): 1-14. http://dx.doi.org/10.1080/19490976.2020.1712983. Published • 10.1080/19490976.2020.1712983 Cláudia Brito; Francisco S. Mesquita; Christopher K. E. Bleck; James R. Sellers; Didier Cabanes; Sandra Sousa. "Perfringolysin O-Induced Plasma Membrane Pores Trigger Actomyosin Remodeling and Endoplasmic Reticulum Redistribution". Toxins (2019): https://doi.org/10.3390/toxins11070419. Published • 10.3390/toxins11070419 Cláudia Brito; Didier Cabanes; Francisco Sarmento Mesquita; Sandra Sousa. "Mechanisms protecting host cells against bacterial pore-forming toxins". Cellular and Molecular Life Sciences 76 7 (2019): 1319-1339. https://doi.org/10.1007/s00018-018-2992-8. Published • 10.1007/s00018-018-2992-8 Ana Catarina Costa; Filipe Carvalho; Didier Cabanes; Sandra Sousa. "Stathmin recruits tubulin to Listeria monocytogenes-induced actin comets and promotes bacterial dissemination". Cellular and Molecular Life Sciences 76 5 (2019): 961-975. https://doi.org/10.1007/s00018-018-2977-7. Published • 10.1007/s00018-018-2977-7 Rita Pombinho; Sandra Sousa; Didier Cabanes. "Scavenger Receptors: Promiscuous Players during Microbial Pathogenesis". Critical Reviews in Microbiology 44 6 (2018): 685-700. https://doi.org/10.1080/1040841X.2018.1493716. Published • 10.1080/1040841X.2018.1493716 Pinheiro, Jorge; Lisboa, Johnny; Pombinho, Rita; Carvalho, Filipe; Carreaux, Alexis; Brito, Cláudia; Pöntinen, Anna; et al. "MouR controls the expression of the Listeria monocytogenes Agr system and mediates virulence". Nucleic Acids Research (2018): http://dx.doi.org/10.1093/nar/gky624. 10.1093/nar/gky624 Carvalho, Filipe; Sousa, Sandra; Cabanes, Didier. "l -Rhamnosylation of wall teichoic acids promotes efficient surface association of Listeria monocytogenes virulence factors InlB and Ami through interaction with GW domains". Environmental Microbiology 20 11 (2018): 3941-3951. http://dx.doi.org/10.1111/1462-2920.14351. 10.1111/1462-2920.14351 Cruz, R.; Pereira-Castro, I.; Almeida, M.T.; Moreira, A.; Cabanes, D.; Sousa, S.. "Epithelial keratins modulate cMet expression and signaling and promote InlB-mediated Listeria monocytogenes infection of HeLa cells". Frontiers in Cellular and Infection Microbiology 8 MAY (2018): http://www.scopus.com/inward/record.url?eid=2-s2.0-85046878080&partnerID=MN8TOARS. Published • 10.3389/fcimb.2018.00146 Mesquita, F.S.; Brito, C.; Cabanes, D.; Sousa, S.. "Control of cytoskeletal dynamics during cellular responses to pore forming toxins". Communicative and Integrative Biology 10 5-6 (2017): http://www.scopus.com/inward/record.url?eid=2-s2.0-85030151258&partnerID=MN8TOARS. Published • 10.1080/19420889.2017.1349582 Mesquita, F.S.; Brito, C.; Mazon Moya, M.J.; Pinheiro, J.C.; Mostowy, S.; Cabanes, D.; Sousa, S.. "Endoplasmic reticulum chaperone Gp96 controls actomyosin dynamics and protects against pore-forming toxins". EMBO Reports 18 2 (2017): 303-318. http://www.scopus.com/inward/record.url?eid=2-s2.0-85007487818&partnerID=MN8TOARS. Published • 10.15252/embr.201642833 Pombinho, R.; Camejo, A.; Vieira, A.; Reis, O.; Carvalho, F.; Almeida, M.T.; Pinheiro, J.C.; Sousa, S.; Cabanes, D.. "Listeria monocytogenes CadC regulates cadmium efflux and fine-tunes lipoprotein localization to escape the host immune response and promote infection". Journal of Infectious Diseases 215 9 (2017): 1468-1479. http://www.scopus.com/inward/record.url?eid=2-s2.0-85021919420&partnerID=MN8TOARS. Published • 10.1093/infdis/jix118 Pinheiro, J.; Reis, O.; Vieira, A.; Moura, I.M.; Moreno, L.Z.; Carvalho, F.; Pucciarelli, M.G.; et al. "Listeria monocytogenes encodes a functional ESX-1 secretion system whose expression is detrimental to in vivo infection". Virulence 8 6 (2017): 993-1004. http://www.scopus.com/inward/record.url?eid=2-s2.0-84992702646&partnerID=MN8TOARS. Published • 10.1080/21505594.2016.1244589 do Vale, A.; Cabanes, D.; Sousa, S.. "Bacterial toxins as pathogen weapons against phagocytes". Frontiers in Microbiology 7 FEB (2016): http://www.scopus.com/inward/record.url?eid=2-s2.0-84962082244&partnerID=MN8TOARS. Published • 10.3389/fmicb.2016.00042 Carvalho, F.; Atilano, M.L.; Pombinho, R.; Covas, G.; Gallo, R.L.; Filipe, S.R.; Sousa, S.; Cabanes, D.. "L-Rhamnosylation of Listeria monocytogenes Wall Teichoic Acids Promotes Resistance to Antimicrobial Peptides by Delaying Interaction with the Membrane". PLoS Pathogens 11 5 (2015): http://www.scopus.com/inward/record.url?eid=2-s2.0-84930354332&partnerID=MN8TOARS. Published • 10.1371/journal.ppat.1004919 Almeida, M.T.; Mesquita, F.S.; Cruz, R.; Osório, H.; Custódio, R.; Brito, C.; Vingadassalom, D.; et al. "Src-dependent tyrosine phosphorylation of non-muscle myosin heavy chain-IIA restricts Listeria monocytogenes cellular infection". Journal of Biological Chemistry 290 13 (2015): 8383-8395. http://www.scopus.com/inward/record.url?eid=2-s2.0-84925760833&partnerID=MN8TOARS. Published • 10.1074/jbc.M114.591313 Sousa, S.; Mesquita, F.S.; Cabanes, D.. "Old war, new battle, new fighters!". Journal of Infectious Diseases 211 9 (2015): 1361-1363. http://www.scopus.com/inward/record.url?eid=2-s2.0-84928914684&partnerID=MN8TOARS. Published • 10.1093/infdis/jiu521 Carvalho, F.; Sousa, S.; Cabanes, D.. "How Listeria monocytogenes organizes its surface for virulence". Frontiers in cellular and infection microbiology 4 (2014): http://www.scopus.com/inward/record.url?eid=2-s2.0-84924841119&partnerID=MN8TOARS. Published • 10.3389/fcimb.2014.00048 Leitão, E.; Costa, A.C.; Brito, C.; Costa, L.; Pombinho, R.; Cabanes, D.; Sousa, S.. "Listeria monocytogenes induces host DNA damage and delays the host cell cycle to promote infection". Cell Cycle 13 6 (2014): 928-940. http://www.scopus.com/inward/record.url?eid=2-s2.0-84897994129&partnerID=MN8TOARS. Published • 10.4161/cc.27780 Henriques, A.; Carvalho, F.; Pombinho, R.; Reis, O.; Sousa, S.; Cabanes, D.. "PCR-based screening of targeted mutants for the fast and simultaneous identification of bacterial virulence factors". BioTechniques 53 1 (2012): http://www.scopus.com/inward/record.url?eid=2-s2.0-85003043352&partnerID=MN8TOARS. Published • 10.2144/000113906 Martins, M.; Custod´io, R.; Camejo, A.; Almeida, M.T.; Cabanes, D.; Sousa, S.. "Listeria monocytogenes triggers the cell surface expression of Gp96 protein and interacts with its N terminus to support cellular infection". Journal of Biological Chemistry 287 51 (2012): 43083-43093. http://www.scopus.com/inward/record.url?eid=2-s2.0-84871163192&partnerID=MN8TOARS. Published • 10.1074/jbc.M112.422568 Camejo, A.; Carvalho, F.; Reis, O.; Leitão, E.; Sousa, S.; Cabanes, D.. "The arsenal of virulence factors deployed by Listeria monocytogenes to promote its cell infection cycle". Virulence 2 5 (2011): 379-394. http://www.scopus.com/inward/record.url?eid=2-s2.0-80053927534&partnerID=MN8TOARS. Published • 10.4161/viru.2.5.17703 Reis, O.; Sousa, S.; Camejo, A.; Villiers, V.; Gouin, E.; Cossart, P.; Cabanes, D.. "LapB, a novel Listeria monocytogenes LPXTG surface adhesin, required for entry into eukaryotic cells and virulence". Journal of Infectious Diseases 202 4 (2010): 551-562. http://www.scopus.com/inward/record.url?eid=2-s2.0-77954706300&partnerID=MN8TOARS. Published • 10.1086/654880 Camejo, A.; Buchrieser, C.; Couvé, E.; Carvalho, F.; Reis, O.; Ferreira, P.; Sousa, S.; Cossart, P.; Cabanes, D.. "In vivo transcriptional profiling of Listeria monocytogenes and mutagenesis identify new virulence factors involved in infection". PLoS Pathogens 5 5 (2009): http://www.scopus.com/inward/record.url?eid=2-s2.0-67249147690&partnerID=MN8TOARS. Published • 10.1371/journal.ppat.1000449 Sousa, S.; Cabanes, D.; Bougnères, L.; Lecuit, M.; Sansonetti, P.; Tran-van-nhieu, G.; Cossart, P.. "Src, cortactin and Arp2/3 complex are required for E-cadherin-mediated internalization of Listeria into cells". Cellular Microbiology 9 11 (2007): 2629-2643. http://www.scopus.com/inward/record.url?eid=2-s2.0-34848843528&partnerID=MN8TOARS. Published • 10.1111/j.1462-5822.2007.00984.x Boneca, I.G.; Dussurget, O.; Cabanes, D.; Nahori, M.-A.; Sousa, S.; Lecuit, M.; Psylinakis, E.; et al. "A critical role for peptidoglycan N-deacetylation in Listeria evasion from the host innate immune system". Proceedings of the National Academy of Sciences of the United States of America 104 3 (2007): 997-1002. http://www.scopus.com/inward/record.url?eid=2-s2.0-33846526208&partnerID=MN8TOARS. Published • 10.1073/pnas.0609672104 Cabanes, D.; Sousa, S.; Cebriá, A.; Lecuit, M.; García-Del Portillo, F.; Cossart, P.. "Gp96 is a receptor for a novel Listeria monocytogenes virulence factor, Vip, a surface protein". EMBO Journal 24 15 (2005): 2827-2838. http://www.scopus.com/inward/record.url?eid=2-s2.0-23844459938&partnerID=MN8TOARS. Published • 10.1038/sj.emboj.7600750 Sousa, S.; Cabanes, D.; Archambaud, C.; Colland, F.; Lemichez, E.; Popoff, M.; Boisson-Dupuis, S.; et al. "ARHGAP10 is necessary for a-catenin recruitment at adherens junctions and for Listeria invasion". Nature Cell Biology 7 10 (2005): 954-960. http://www.scopus.com/inward/record.url?eid=2-s2.0-27144507346&partnerID=MN8TOARS. Published • 10.1038/ncb1308 Sousa, S.; Lecuit, M.; Cossart, P.. "Microbial strategies to target, cross or disrupt epithelia". Current Opinion in Cell Biology 17 5 SPEC. IS (2005): 489-498. http://www.scopus.com/inward/record.url?eid=2-s2.0-24644435107&partnerID=MN8TOARS. Published • 10.1016/j.ceb.2005.08.013 Pizarro-Cerdá, J.; Sousa, S.; Cossart, P.. "Exploitation of host cell cytoskeleton and signalling during Listeria monocytogenes entry into mammalian cells.". Comptes rendus biologies 327 6 (2004): 523-531. http://www.scopus.com/inward/record.url?eid=2-s2.0-4644360131&partnerID=MN8TOARS. Published Sousa, S.; Cabanes, D.; El-Amraoui, A.; Petit, C.; Lecuit, M.; Cossart, P.. "Unconventional myosin VIIa and vezatin, two proteins crucial for Listeria entry into epithelial cells". Journal of Cell Science 117 10 (2004): 2121-2130. http://www.scopus.com/inward/record.url?eid=2-s2.0-2942549121&partnerID=MN8TOARS. Published • 10.1242/jcs.01066 Pizarro-Cerdá, J.; Sousa, S.; Cossart, P.. "Exploitation of host cell cytoskeleton and signalling during Listeria monocytogenes entry into mammalian cells". Comptes Rendus - Biologies 327 2 (2004): 115-123. http://www.scopus.com/inward/record.url?eid=2-s2.0-1342330320&partnerID=MN8TOARS. Published • 10.1016/j.crvi.2003.11.007 Pizarro-Cerdá, J.; Sousa, S.; Cossart, P.. "Erratum: "Exploitation of host cell cytoskeleton and signalling during Listeria monocytogenes entry into mammalian cells" (Comptes Rendus Biologies (2004) vol. 327 (2) (115-123) 10.1016/j.crvi.2004.06.001)". Comptes Rendus - Biologies 327 6 (2004): http://www.scopus.com/inward/record.url?eid=2-s2.0-3242764544&partnerID=MN8TOARS. 10.1016/j.crvi.2004.05.001 Leroy, A.; Vanzo, N.F.; Sousa, S.; Dreyfus, M.; Carpousis, A.J.. "Function in Escherichia coli of the non-catalytic part of RNase E: Role in the degradation of ribosome-free mRNA". Molecular Microbiology 45 5 (2002): 1231-1243. http://www.scopus.com/inward/record.url?eid=2-s2.0-0036047092&partnerID=MN8TOARS. Published • 10.1046/j.1365-2958.2002.03104.x Sousa, S.; Marchand, I.; Dreyfus, M.. "Autoregulation allows Escherichia coli RNase E to adjust continuously its synthesis to that of its substrates". Molecular Microbiology 42 3 (2001): 867-878. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035168335&partnerID=MN8TOARS. Published • 10.1046/j.1365-2958.2001.02687.x
Preprint	Ricardo Monteiro; Tatiana B. Cereija; Rita Pombinho; Sandra Sousa; João H. Morais-Cabral; Didier Cabanes. "Molecular properties of RmlT, a wall teichoic acid rhamnosyltransferase that modulatesListeria monocytogenesvirulence and antibiotic resistance". 2024. https://doi.org/10.1101/2024.01.23.574818. 10.1101/2024.01.23.574818
Thesis / Dissertation	Sousa, Sandra. "Bases moléculaires et cellulaires de l'entrée de Listeria monocytogenes dans les cellules de mammifères". PhD, Université Paris VII Denis Diderot UFR Sciences du Vivant, 2004. Sousa, Sandra. "Biological consequences of variation in RNase E concentration, an essential Escherichia coli endonuclease.". Master, Université Paris VII Denis Diderot UFR Sciences du Vivant, 2000.
Working paper	Cláudia Brito; Francisco S. Mesquita; Daniel S. Osório; Joana Pereira; Neil Billington; James R. Sellers; Ana X. Carvalho; Didier Cabanes; Sandra Sousa. 2020. "Src-dependent NM2A tyrosine-phosphorylation regulates actomyosin dynamics". https://doi.org/10.1101/2020.08.12.246090. 10.1101/2020.08.12.246090

Activities

Oral presentation

	Presentation title	Event name Host (Event location)
2017/11	Role for host cytoskeletal components during Listeria monocytogenes invasion of epithelial cells and bacterial pore-forming toxins attack.	External Seminars Sackler School of Biomedical Sciences, Tufts University John Leong (Boston, United States)
2016	Host survival responses to the pore-forming toxin LLO: cytoskeleton and ER partnership.	ISOPOL XIX. Problems of Listeriosis EMBO Conference (Paris, France)
2015/06	Host survival responses to the pore-forming toxin LLO: cytoskeleton and ER partnership.	ETOX'17 European Workshop on Bacterial Protein Toxins ETOX'17 European Workshop on Bacterial Protein Toxins (Braga, Portugal)
2007/12	Listeria: subverting cellular functions to get into host cells	Seminar Series IBMC Didier Cabanes (Porto, Portugal)
2004/03	Entry of Listeria monocytogenes in non-phagocytic cells: role for myosin VIIa and Vezatin.	External Seminars Mayo Clinic Marc McNiven (Rochester, United States)
2004/03	Entry of Listeria monocytogenes in non-phagocytic cells: role for myosin VIIa and Vezatin.	External Seminar National Institutes of Health, Rocky Mountain Laboratories Ted Hackstadt (Hamilton, United States)

Supervision

	Thesis Title Role	Degree Subject (Type) Institution / Organization
2020/01 - 2024/12	Mechanisms of host resistance to plasma membrane damage induced by pneumolysin attack Supervisor	Biologia Molecular e Celular (PhD) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2020/02 - 2021/01	Exploring the role of Vav3 in the repair of toxin-induced plasma membrane damage. Supervisor	Mestrado integrado em Engenharia Biológica (Master) Universidade do Minho, Portugal Universidade do Minho Escola de Engenharia, Portugal
2015 - 2020/04	Host cell cytoskeleton responses against bacterial pore-forming toxins Supervisor	Biologia Molecular e Celular (PhD) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2019/01 - 2019/07	Cellular localisation of pore-forming toxins upon time of intoxication Supervisor	ERASMUS (Degree) Università degli Studi dell'Aquila, Italy
2014/02 - 2018/09	Unravelling the role of keratins in bacterial infections Supervisor	Ciências Biomédicas (PhD) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2009/01 - 2015/09	Identification of new host signaling pathways hijacked by bacterial pathogens during infection Supervisor	Ciências Biomédicas (PhD) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2011 - 2015	Studies on the mechanisms of maturation and membrane disassembly of vacuoles containing invasive bacteria Co-supervisor	Biologia Básica e Aplicada (PhD) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2012/09 - 2013/10	Identification of novel non-muscle myosin IIA (NMIIA) interacting partners Supervisor	Biologia Celular e Molecular (Master) Universidade do Porto Faculdade de Ciências, Portugal
2012/09 - 2013/09	Effects induced by Listeria monocytogenes infection on the host cell cycle Supervisor	Bioquímica (Master) Universidade do Porto Faculdade de Ciências, Portugal
2013/01 - 2013/07	Identification of host proteins hijacked by Listeria monocytogenes to interfere with cell cycle progression Supervisor	ERASMUS (Degree) Université Paris VII Denis Diderot UFR Sciences du Vivant, France
2012/01 - 2012/07	Identification of bacterial factors required to induce host cell cycle delay Supervisor	ERASMUS (Degree) Université Paris VII Denis Diderot UFR Sciences du Vivant, France
2006 - 2010	identification of new Listeria monocytogenes virulence factors: comparative genomics and transcriptomics Co-supervisor	Ciências Biomédicas (PhD) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2006 - 2010	Identification and characterization of new Listeria monocytogenes virulence factors Co-supervisor	Ciências Biomédicas (PhD) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2008/09 - 2009/12	Molecular characterization of the Vip-Gp96 interaction. Supervisor	Bioquímica (Master) Universidade do Porto Faculdade de Ciências, Portugal Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2007/01 - 2008/12	Tyrosine-phosphorylation profile of Listeria monocytogenes infected-cells: Identification of new host factors hijacked to promote infection Supervisor	Medicina e Oncologia Molecular (Master) Universidade do Porto Faculdade de Medicina, Portugal

Event organisation

	Event name Type of event (Role)	Institution / Organization
2014/10 - 2015/06/24	Organizing Committee ETO'17-European Workshop on Bacterial Protein Toxins (2015/06/20 - 2015/06/24) Workshop (Member of the Organising Committee)
2010/01 - 2010/09	Organization of International Workshop on Host-Pathogen Interactions (2010/09/27 - 2010/09/27) Symposium (Member of the Organising Committee)	Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal

Jury of academic degree

	Topic Role	Candidate name (Type of degree) Institution / Organization
2018/12	Photobacterrium damselae subp. damselae: analysis of the genetic diversity and characterization of a conserved two-component system regulating virulence (Thesis) Main arguer	Mateus de Souza Terceti (PhD) Universidade de Santiago de Compostela - Campus Vida, Spain
2018	Identification and characterization of CteG, a novel Chlamydia trachomatis type III secretion effector protein (Thesis) Main arguer	Sara Raquel Vilela Pais (PhD) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2016/03	Functional analysis of inclusion membrane proteins of Chlamydia trachomatis (Thesis) Arguer	Filipe Manuel baeta da Silva Almeida (PhD) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2014/11	Manipulation of host cell functions by Samonella: the role of the bacterial effector protein SteA (Thesis) Arguer	Lia Dora David Domingues (PhD) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2014	Intracellular trafficking of AIP56, a bacterial AB toxin targetting NK-kB (Thesis) Main arguer	Liliana Marisa Gonçalves Pereira (PhD) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2013/01	Identification of nuclear envelope proteins involved in actin-dependent nuclear movement (Thesis) Arguer	Daniel Osório (PhD) Universidade do Porto Faculdade de Medicina, Portugal
2012	Characterisation of the oligomerisation-dependent signalling function of the LD domain of the TARP virulence factor from Chlamydia (Thesis) Main arguer	Ana Celeste Teixeira Nogueira (Master) Universidade Católica Portuguesa Escola Superior de Biotecnologia, Portugal
2011	Development of a mammalian cell model to study AIP56 (Thesis) Main arguer	Silvia Liliana Gomes Mota (Master) Universidade de Aveiro Departamento de Biologia, Portugal
2011	The characterisation of the possible interaction between the Chlamydia effector TARP and its putative chaperone CT043 (Thesis) Main arguer	António José santos Tedim Sousa Pedrosa (Master) Universidade Católica Portuguesa Escola Superior de Biotecnologia, Portugal
2011	Investigation of the anti-apoptotic activity of the Salmonella NleH homologues (Thesis) Main arguer	Joana Lopes Abreu Miranda (Master) Universidade Católica Portuguesa Escola Superior de Biotecnologia, Portugal
2009	Characterization of CD6 splicing regulatory mechanism governed by activation-induced splicing sensors (Thesis) Main arguer	Vânia Raquel Gomes Glória (Master) Universidade do Porto Faculdade de Ciências, Portugal
2008	The role of exoribonucleases in Growth phase control and their function in the degradation of RNA (Thesis) Arguer	José Eduardo Marques Andrade (PhD) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Course / Discipline taught

	Academic session	Degree Subject (Type)	Institution / Organization
2017 - Current	Methods of forward genetics	Biologia Molecular e Celular (Doutoramento)	Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2008 - Current	Host cell usurpation by bacterial pathogens	Biologia Básica e Aplicada (Doutoramento)	Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2006 - Current	Host cell usurpation by bacterial pathogens	Bioquímica (Mestrado integrado)	Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal

Evaluation committee

	Activity description Role	Institution / Organization	Funding entity
2022/08 - Current	Grant Evaluation for Deutsche Forschungsgemeinschaft-German Research Foundation Evaluator	Deutsche Forschungsgemeinschaft, Germany
2019/12 - Current	Grant Evaluation for Swiss National Science Foundation (SNSF- Switzerland) Evaluator	Schweizerischer Nationalfonds zur Förderung der wissenschaftlichen Forschung, Switzerland
2019/06 - Current	Grant Evaluation for Swiss National Science Foundation (SNSF- Switzerland) Evaluator	Schweizerischer Nationalfonds zur Förderung der wissenschaftlichen Forschung, Switzerland
2020/10 - 2020/10	Grant Evaluation for projects for ETH Zurich (Switzerland) Evaluator	Eidgenössische Technische Hochschule Zürich, Switzerland	Eidgenössische Technische Hochschule Zürich
2020/05 - 2020/05	Grant Evaluations for FCT- Covid 19 Call Evaluator	Fundação para a Ciência e a Tecnologia, Portugal	Fundação para a Ciência e a Tecnologia
2019 - 2019	Grant Evaluation for Agence Nationale de la Recherche (ANR-France) Evaluator	Agence nationale de la recherche, France
2017 - 2017	Grant Evaluation for Agence Nationale de la Recherche (ANR-France) Evaluator	Agence nationale de la recherche, France
2016 - 2016	Grant Evaluation for Agence Nationale de la Recherche (ANR-France) Evaluator	Agence nationale de la recherche, France

Journal scientific committee

	Journal title (ISSN)	Publisher
2023 - Current	Frontiers in Bacteriology
2021 - Current	Toxins (2072-6651)	MDPI
2021 - Current	Frontiers in Cellular and Infection Microbiology (2235-2988)	Frontiers

Other jury / evaluation

	Activity description	Institution / Organization
2010 - Current	Referee for peer reviewed journals: - Cellular Microbiology - EMBO Reports - Infection & Immunity - Microbes and Infection - PLoS Pathogens - International Journal of Medical Microbiology - Future Microbiology - Virulence - Toxins - Research in Microbiology - Scientific Reports - Molecular Microbiology - Microbial Pathogenesis

Distinctions

Award

2014	ESCMID Research Grant Award European Society of Clinical Microbiology and Infectious Diseases, Switzerland
2011	Role Model for the Next Generations Câmara Municipal do Porto, Portugal
2005	L'Oréal for Women in Science Fundação para a Ciência e a Tecnologia, Portugal Fondation L'Oréal, France