Benedita Pinheiro (CB17-E1B7-4EE7)

Curriculum Vitae

Benedita Pinheiro

I am a researcher in at the Applied Molecular Biosciences Unit & i4HB Associated Laboratory-NOVA School of Science and Technology (UCIBIO-FCT-NOVA). I’m a member of the UCIBIO Macromolecular Crystallography and Functional Glycobiology Labs. I have been responsible for applying structural and sequence bioinformatics for the identification, construct design and recombinant expression of carbohydrate-binding and carbohydrate-active proteins. I have specialized in the analysis of these proteins using glycan-microarrays, x-ray crystallography and several biophysical characterization techniques such as Isothermal titration calorimetry (ITC) and Microscale Thermophoresis (MST). I also give assistance in molecular biology in both groups. PHD TRAINING My PhD was focused on the study at the molecular and atomic level of the cohesin-dockerin interaction, important for the assembly of a multi-enzymatic complex involved in the degradation of the plant cell wall by cellulolytic bacteria, the cellulosome. For this, I gained expertise in Molecular Biology essential for the recombinant production of the cohesin and dockerin proteins and mutational analysis of the function. In the initial study, I specially devised and implemented an essay using Non-Denaturing Gel Electrophoresis (NGE) for the study of cohesin-dockerin pairs and complemented with a thermodynamic analysis of the interaction by Isothermal Titration Calorimetry (ITC) and structurally characterization by X-ray Crystallography. In my PhD, I was also involved in the cloning and production of other protein modules constituents of the C. thermocellum cellulosome like esterases, glycohydrolases and carbohydrate-binding modules (CBM). POST-DOC TRAINING For my Post-Doc, I decided to deepen my knowledge in X-ray Crystallography moving to the group of Prof Maria João Romão. During this time, I initiated a collaboration with Dr Angelina Palma, having been introduced to glycan microarrays. I have also received training in HTP techniques for protein production (EMBO course) and Structural Bioinformatics (EMBL-EBI course). RESEARCH CONTRIBUTIONS I was involved in setting up molecular biology techniques and protocols in the Macromolecular Crystallography laboratory, which opened new avenues of research. I implemented the protein microarray technology in the Functional Glycobiology laboratory within the scope of a project grant funded by FCT to establish the proof-of-concept. I have been involved in several funded projects (2022.06104.PTDC, PTDC/BIA-MIB/31730/2017, PTDC/BIA-MIB/31730/2017, PTDC/QUI-QUI/112537/2009) where X-ray crystallography is combined with glycan microarray analysis, revealed the unique selective binding of several carbohydrate interacting proteins from bacteria from the rumen and from the human gut. I’m currently part of the internationally funded project GLYCOTwinning (HORIZON-WIDERA-2021-101079417). SUPERVISION, I have been the supervisor of three MSc student Thesis and was involved in the training of four PhD students, 3 MSc researchers and 7 undergraduate students. I have been involved in the tutoring of students for the program Verão com Ciência. TEACHING I have given regular support to Biochemistry and Structural Biology classes. I annually teach practical classes for the subject “Metabolismo e Regulação”. OUTREACH I participate in the FCT Open day and European Researcher Night. I am responsible for the Functional Glycobiology website (https://glycolab.webhost.fct.unl.pt/). Our divulgation videos are available at https://glycolab.webhost.fct.unl.pt/index.php/acucares-mais-do-que-apenas-doces-moleculas/.

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Identification

Personal identification

Full name: Benedita Pinheiro

Citation names

Pinheiro, Benedita
Pinheiro, B. A.

Author identifiers

Ciência ID: CB17-E1B7-4EE7
ORCID iD: 0000-0002-3045-077X

Email addresses

b.pinheiro@fct.unl.pt (Professional)

Websites

https://glycolab.webhost.fct.unl.pt/ (Professional)

Knowledge fields

Natural sciences - Biological Sciences - Biochemistry

Languages

Language	Speaking	Reading	Writing	Listening
English	Advanced (C1)	Advanced (C1)	Advanced (C1)	Advanced (C1)
French	Beginner (A1)	Intermediate (B1)	Beginner (A1)	Beginner (A1)
Portuguese (Mother tongue)
Creole and Pidgin, Portuguese-based (Other)

Education

	Degree	Classification
2010 Concluded	Tese de Doutoramento em Ciência e Tecnologia Animal/PhD in Animal Science and Technology (Doutoramento) Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal "Novel insight into the mechanism of cellulosome assembly and plant cell wall hydrolysis in anaerobic bacteria" (THESIS/DISSERTATION)	Por unanimidade/By unanimity
2009 Concluded	Certificate in Advanced English (Diploma de especialização) Cambridge School Portugal, Portugal	B
2008/04/08 - 2008/04/18 Concluded	VII Course on Laboratory Animal Science (Outros) Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
2004 Concluded	Bioquímica (Licenciatura) Universidade de Lisboa Faculdade de Ciências, Portugal "Adesão de Desulfovibrio alaskensis a superfícies de vidro" (THESIS/DISSERTATION)	16 (maximum 20)

Affiliation

Science

	Category Host institution	Employer
2018/12/19 - Current	Contracted Researcher (Research)	REQUIMTE Unidade de Ciências Biomoleculares Aplicadas, Portugal

Others

	Category Host institution	Employer
2010/10/01 - Current	Postdoctoral researcher	REQUIMTE Unidade de Ciências Biomoleculares Aplicadas, Portugal

2010/02/15 - 2010/09/30	FCT-UNL Requimte Research fellow.	Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal

2006/01/01 - 2009/12/31	PhD student at the Laboratory of Nutrition under supervision of Prof Carlos Fontes	Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal

2009/01/09 - 2009/04/09	Traineeship (3 months) in the laboratory of Professor Harry Gilbert	Newcastle University Institute for Cell and Molecular Biosciences, United Kingdom

2008/06/01 - 2008/09/30	Traineeship (3 months) in the laboratory of Professor Harry Gilbert. Consolidation of the calorimetric data of protein complexes under study.	Newcastle University Institute for Cell and Molecular Biosciences, United Kingdom

2007/05/01 - 2007/07/30	Traineeship in the laboratory of Professor Harry Gilbert . Aim was to obtain calorimetric values of the formation of protein-protein complexes. Learned the handling of the isothermal calorimetry titra	Newcastle University Institute for Cell and Molecular Biosciences, United Kingdom

2005/04/01 - 2006/12/31	Research Fellow at the Interdisciplinary Centre of Research in Animal Health (CIISA)	Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal

2004/11/01 - 2005/03/31	Technical Volunteer in Biocorrosion group	Universidade de Lisboa Faculdade de Ciências, Portugal

2003/09/01 - 2004/10/30	Final course trainee in the laboratory of Biocorrosion	Universidade de Lisboa Faculdade de Ciências, Portugal

Projects

Contract

	Designation	Funders
2023/01 - 2027/12	CryoEM@NOVA: a cryo electron microscopy hub enabling drug discovery 101087571	European Commission
2023/03/01 - 2026/02/28	Glicofoldameros como inibidores de toxinas bacterianas 2022.03561.PTDC Universidade Nova de Lisboa, Portugal Associação para a Inovação e Desenvolvimento da FCT, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2023/03/01 - 2026/02/28	Descoberta de proteínas do microbioma humano selectivas para O-glicanos – ferramentas para novas abordagens na investigação em cancro 2022.06104.PTDC Associação para a Inovação e Desenvolvimento da FCT, Portugal Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2021/01/01 - 2025/12/31	Institute for Health and Bioeconomy LA/P/0140/2020 10.54499/LA/P/0140/2020 Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal Universidade Nova de Lisboa, Portugal Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal Unidade de Ciências Biomoleculares Aplicadas, Portugal Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal INESC Microsistemas e Nanotecnologias, Portugal Universidade Nova de Lisboa Associação para a Inovação e Desenvolvimento da FCT, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2022/12 - 2025/11	GLYCOTwinning: Building Networks to Excel in Glycosciences 101079417	European Commission
2022/01/10 - 2023/07/09	Compreender as interações e modulação alostérica do transportador de norepinefrina NET EXPL/BIA-BQM/0011/2021 Associação para a Inovação e Desenvolvimento da FCT, Portugal REQUIMTE Unidade de Ciências Biomoleculares Aplicadas, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2018/09/01 - 2022/08/31	Interação do microbioma humano com hidratos de carbono: diversidade estrutural e reconhecimento de hidratos de carbono no intestino humano e impacto na saúde e nutrição PTDC/BIA-MIB/31730/2017 Associação para a Inovação e Desenvolvimento da FCT, Portugal Universidade de Aveiro, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2019/01/01 - 2019/12/31	Applied Molecular Biosciences Unit UID/Multi/04378/2019 Universidade do Porto Faculdade de Ciências, Portugal Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal Universidade do Porto Instituto de Ciências Tecnologias e Agroambiente, Portugal Unidade de Ciências Biomoleculares Aplicadas, Portugal Universidade Nova de Lisboa Associação para a Inovação e Desenvolvimento da FCT, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2010/10/01 - 2018/12/18	THE CELLULOSOME ASSEMBLY: STRUCTURE, FUNCTION AND SPECIFICITY OF NEW CELLULASES AND SUPRAMOLECULAR COMPLEXES SFRH/BPD/68563/2010	Fundação para a Ciência e a Tecnologia Concluded
2017/01 - 2018/12	Molecular determinants of bacterial cellulosome diversity in different ecological niches Provided by PTCRIS: 144587 Post-doc Fellow	Fundação para a Ciência e a Tecnologia, I.P.
2016/10 - 2017/09	An integrative structural biology approach to characterise the protein-carbohydrate microbial recognition Provided by PTCRIS: 139494 Post-doc Fellow	Fundação para a Ciência e a Tecnologia, I.P.
2013/07 - 2016/06	Modern Structural Biology: Resources for the advancement of in-house X-ray Crystallography 125363 Provided by PTCRIS: 125363	Fundação para a Ciência e a Tecnologia, I.P.
2011/03/01 - 2014/08/31	Microarrays de hidratos de carbono para identificação de novos ligandos no reconhecimento de polissacáridos por módulos proteicos de bactérias. PTDC/QUI-QUI/112537/2009 Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal Associação para a Inovação e Desenvolvimento da FCT, Portugal Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2013/07 - 2014/06	Development of cohesin- microarrays for determining cohesin-dockerin specificities of novel cellulolytic species Provided by PTCRIS: 126085 126085 Principal investigator	Fundação para a Ciência e a Tecnologia, I.P.
2013/07 - 2014/06	Pylori GlycoArrays: Development of microarrays from Helicobacter pylori cell surface glycome toward immunological and serological studies 127236 Provided by PTCRIS: 127236 EXPL/BBB-BQB/0750/2012 Researcher Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal Associação para a Inovação e Desenvolvimento da FCT, Portugal Universidade de Aveiro, Portugal	Fundação para a Ciência e a Tecnologia, I.P. Fundação para a Ciência e a Tecnologia Concluded
2009/01/01 - 2011/12/31	Estrutura e função de novos módulos de ligação a carbohidratos e complexos coesina-doquerina do Clostridium thermocellum PTDC/BIA-PRO/69732/2006 Universidade de Lisboa Centro de Investigação Interdisciplinar em Sanidade Animal, Portugal Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2006/01/01 - 2009/12/31	DEVELOPING AND TESTING TAILOR-MADE CELLULASE AND XYLANASES MULTI-ENZYME COMPLEXES TO IMPROVE THE NUTRITIVE VALUE OF CEREAL-BASED DIETS FOR POULTRY SFRH/BD/25439/2005	Fundação para a Ciência e a Tecnologia Concluded

Outputs

Publications

Book chapter	Correia, Viviana; Costa, Raquel; Trovão, Filipa; Candeias, Alícia; Pinheiro, Benedita. "The bacterial toolkit for glycan recognition in the human gut". In Carbohydrate Chemistry, edited by Amélia Pilar Rauter; Yves Queneau; Palma, Angelina S., 175-191. Royal Society of Chemistry, 2024. Published • 10.1039/bk9781837672844-00175 Ribeiro, Diana O.; Pinheiro, Benedita A.; Carvalho, Ana Luísa; Palma, Angelina S.. "Targeting protein-carbohydrate interactions in plant cell-wall biodegradation: the power of carbohydrate microarrays". In Carbohydrate Chemistry, 159-176. Royal Society of Chemistry, 2017. 10.1039/9781788010641-00159
Conference paper	Correia, Viviana G.; Brás, Joana Luís Armada; Liu, Yan; Silva, Lisete; Zhang, Yibing; Pinheiro, Benedita; Romão, Maria João C.; et al. "An integrative strategy to decipher glycan recognition in the human gut microbiome.". Paper presented in Annual Meeting of the Society for Glycobiology, New Orleans, LA, 2016. Published
Conference poster	Candeias, Alícia; Pinheiro, Benedita; Palma, Angelina S.; Marcelo, Filipa; Coelho, Helena. Corresponding author: Coelho, Helena. "Mucin O-glycan recognition by Salmonella SiiE adhesin for targeted anti-adhesion therapy". Paper presented in 4th Meeting of Young Biophysicists - Biophysics Festival, 2024. Candeias, Alícia; Trovão, Filipa; Oliveira Soares, Cátia; Grosso, Ana Sofia; Marcelo, Filipa; Pinheiro, Benedita; Carvalho, Ana Luísa; Palma, Angelina S.. Corresponding author: Candeias, Alícia. "Structural elucidation of a novel Carbohydrate-binding module from Bacteroides caccae targeting tumor-associated mucin O-glycans". Paper presented in How Time-Resolved X-ray Crystallography is driving Structural Biology forward – Workshop, 2024. Pinheiro, B. A.; Trovão, Filipa; Correia, V.G.; Costa, R.L.; Candeias, Alícia; Di Maio, A.; Feizi, Ten; et al. "Discovery and characterization of O-glycan selective proteins from the human gut microbiota". Paper presented in 7th Iberian Carbohydrate Meeting, 2024. Candeias, Alícia; Trovão, Filipa; Oliveira Soares, Cátia; Grosso, Ana Sofia; Marcelo, Filipa; Pinheiro, Benedita; Carvalho, Ana Luísa; Palma, Angelina S.. Corresponding author: Candeias, Alícia. "A novel Carbohydrate-binding module from Bacteroides caccae targeting tumour-associated mucin O-glycans". Paper presented in 7th Iberian Carbohydrate Meeting, 2024. Leite, Marlene; Candeias, Alícia; Oliveira Soares, Cátia; Pinheiro, Benedita; Dias, Jorge S; Palma, Angelina S.; Marcelo, Filipa; Coelho, H.. Corresponding author: Coelho, H.. "Decoding Salmonella adhesins: Unravelling molecular interactions for novel anti-infection strategies". Paper presented in 7 th Iberian Carbohydrate Meeting, 2024. Candeias, Alícia; Trovão, Filipa; Pinheiro, Benedita; Palma, Angelina S.; Carvalho, Ana Luísa. Corresponding author: Candeias, Alícia. "Structural insights on the recognition of mucin O-glycans by a novel member of family 32 CBM from Bacteroides caccae". Paper presented in 1st GLYCOTwinning Summer School, 2023. Candeias, Alícia; Trovão, Filipa; Pinheiro, Benedita; Palma, Angelina S.; Carvalho, Ana Luísa. Corresponding author: Candeias, Alícia. "Structural basis for recognition of mucin O-glycans by a novel member of family 32 CBM from Bacteroides caccae". Paper presented in XIII Jornadas de Carbohidratos (Spanish Carbohydrate Meeting), 2023. Lopes, Nuno; Trindade, Gonçalo; Pinheiro, Benedita; Palma, Angelina S.; Catarina Brito . Corresponding author: Catarina Brito. "Profiling glycan epitopes in Triple Negative Breast Cancer to explore interactions with tumor-associated macrophages". Paper presented in 14th International Meeting of the Portuguese Carbohydrate Group, 2023. Candeias, Alícia; Trovão, Filipa; Pinheiro, Benedita; Palma, Angelina S.; Carvalho, Ana Luísa. Corresponding author: Candeias, Alícia. "The 3D structure of BC16100-C, a novel protein from Bacteroides caccae, in complex with the Tn antigen reveals the molecular determinants that allow this bacterium to recognise host glycoproteins". Paper presented in GLUPOR14 - 14th International Meeting of the Portuguese Carbohydrate Group, 2023. Engrola, Filipa; Lora Denson; Pinheiro, Benedita; Ci Chu; Santos-Silva, Teresa; Manuel Etzkorn; Viegas, Aldino. "Production of human norepinephrine transporter - hNET - in insect cells". Paper presented in Joint Instruct-iNEXT Course: Integrating Structural Biology Techniques, 2022. Oliveira Soares, Cátia; Natasha Rodrigues; Pinheiro, Benedita; Palma, Angelina S.; Marcelo, Filipa; Coelho, Helena. Corresponding author: Coelho, Helena. "Molecular recognition of host¬glycans by fimbrial adhesins of SalmonellaTyphimurium". Paper presented in Biochemistry Global Summit (IUBMB-FEBS-PABMB 2022) Lisboa (Portugal), 2022. Trovão, Filipa; Candeias, Alícia; Pinheiro, Benedita; Palma, Angelina S.; Carvalho, Ana Luísa. Corresponding author: Candeias, Alícia. "Structural insights on the glycan-binding function of a novel protein from Bacteroides caccae, a commensal bacterium from the human gut". Paper presented in 2nd National Crystallographic Meeting, 2022. Oliveira Soares, Cátia; Natasha Rodrigues; Pinheiro, Benedita; Palma, Angelina S.; Marcelo, Filipa; Coelho, Helena. Corresponding author: Coelho, Helena. "Decoding the adhesion step of Salmonella Typhimurium to host-glycans". Paper presented in First Summer School in Biomedical Glycoscience, 2022. Trovão, Filipa; Candeias, Alícia; Pinheiro, Benedita; Liu, Yan; Carvalho, Ana Luísa; Palma, Angelina S.. Corresponding author: Trovão, Filipa. "Structural elucidation of novel glycan-binding proteins from Bacteroides caccae - a resident of the human gut microbiota". Paper presented in Diamond-CCP4 Data Collection and Structure Solution Workshop, 2021. Pereira, J. R. A.; Correia, V.G.; Carvalho, A. L.; Palma, A.S.; Romão, M. J.; Pinheiro, B. A.. "Cohesin microarrays: A tool to unveil new cellulosome species". Paper presented in National Meeting of Portuguese Students in Biochemistry (ENEBIOQ VII), 2014.
Journal article	Galrinho, Miguel; Silva, Lisete; Lopes, Guido R.; Bernardo A. C. Ferreira; Valente, Sara A.; Isabel Ferreira; Pinheiro, Benedita; et al. Corresponding author: Passos, Claudia P.. "The study of galactomannans with different molecular weights and their ability to form microparticles suitable for pulmonary delivery". Carbohydrate Polymers (2024): Under revision Sofia F. Reis; Susana Messias; Rita Bastos; Vítor J. Martins; Viviana G. Correia; Benedita A. Pinheiro; Lisete M. Silva; et al. "Structural differences on cell wall polysaccharides of brewer's spent Saccharomyces and microarray binding profiles with immune receptors". Carbohydrate Polymers 301 (2023): 120325-120325. http://dx.doi.org/10.1016/j.carbpol.2022.120325. 10.1016/j.carbpol.2022.120325 Sofia F. Reis; Vitor J. Martins; Rita Bastos; Tânia Lima; Viviana G. Correia; Benedita A. Pinheiro; Lisete M. Silva; et al. "Feasibility of Brewer’s Spent Yeast Microcapsules as Targeted Oral Carriers". Foods (2023): https://doi.org/10.3390/foods12020246. 10.3390/foods12020246 Trovão, Filipa; Correia, Viviana G.; Lourenço, Frederico M.; Ribeiro, Diana O.; Carvalho, Ana Luísa; Palma, Angelina S.; Pinheiro, Benedita A.. "The structure of a Bacteroides thetaiotaomicron carbohydrate-binding module provides new insight into the recognition of complex pectic polysaccharides by the human microbiome". Journal of Structural Biology: X 7 (2023): 100084. http://dx.doi.org/10.1016/j.yjsbx.2022.100084. 10.1016/j.yjsbx.2022.100084 Correia VG; Trovão F; Pinheiro BA; Brás JLA; Silva LM; Nunes C; Coimbra MA; et al. "Mapping Molecular Recognition of ß1,3-1,4-Glucans by a Surface Glycan-Binding Protein from the Human Gut Symbiont Bacteroides ovatus.". Microbiology spectrum (2021): http://europepmc.org/abstract/med/34817219. 10.1128/spectrum.01826-21 Gomes, Ana Sara; Ramos, Helena; Gomes, Sara; Loureiro, Joana B.; Soares, Joana; Barcherini, Valentina; Monti, Paola; et al. "SLMP53-1 interacts with wild-type and mutant p53 DNA-binding domain and reactivates multiple hotspot mutations". Biochimica et Biophysica Acta (BBA) - General Subjects 1864 1 (2020): 129440. http://dx.doi.org/10.1016/j.bbagen.2019.129440. 10.1016/j.bbagen.2019.129440 Pandeirada CO; Maricato É; Ferreira SS; Correia VG; Pinheiro BA; Evtuguin DV; Palma AS; et al. "Structural analysis and potential immunostimulatory activity of Nannochloropsis oculata polysaccharides.". Carbohydrate polymers (2019): http://europepmc.org/abstract/med/31320077. 10.1016/j.carbpol.2019.06.001 Gomes, Ana; Trovão, Filipa; Andrade Pinheiro, Benedita; Freire, Filipe; Gomes, Sara; Oliveira, Carla; Domingues, Lucília; et al. "The Crystal Structure of the R280K Mutant of Human p53 Explains the Loss of DNA Binding". International Journal of Molecular Sciences 19 4 (2018): 1184. http://dx.doi.org/10.3390/ijms19041184. 10.3390/ijms19041184 Pinheiro, B. A.; Bras, J. L.; Bule, P.; Pires, V. M.; Romao, M. J.; Alves, V. D.; Palma, A. S.; Fontes, C. M.; Carvalho, A. L.. "Probing the unique molecular mechanisms used by Clostridium thermocellum for Cellulosome function". European Biophysics Journal With Biophysics Letters 46 (2017): S170-S170. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000416406201101&KeyUID=WOS:000416406201101. Brás JL; Pinheiro BA; Cameron K; Cuskin F; Viegas A; Najmudin S; Bule P; et al. "Diverse specificity of cellulosome attachment to the bacterial cell surface.". (2016): http://europepmc.org/abstract/med/27924829. 10.1038/srep38292 Pinheiro, Benedita Andrade; Armada Bras, Joana Luis; Najmudin, Shabir; Carvalho, Ana Luisa; Ferreira, Luis M. A.; Prates, Jose A. M.; Godinho Andrade Fontes, Carlos Mendes. "Flexibility and specificity of the cohesin-dockerin interaction: implications for cellulosome assembly and functionality". Biocatalysis and Biotransformation 30 3 (2012): 309-315. 10.3109/10242422.2012.681854 Najmudin, Shabir; Pinheiro, Benedita A.; Prates, Jose A. M.; Gilbert, Harry J.; Romao, Maria J.; Fontes, Carlos M. G. A.. "Putting an N-terminal end to the Clostridium thermocellum xylanase Xyn10B story: Crystal structure of the CBM22-1-GH10 modules complexed with xylohexaose". Journal of Structural Biology 172 3 (2010): 353-362. 10.1016/j.jsb.2010.07.009 Pinheiro, Benedita A.; Gilbert, Harry J.; Sakka, Kazutaka; Sakka, Kazuo; Fernandes, Vania O.; Prates, Jose A. M.; Alves, Victor D.; et al. "Functional insights into the role of novel type I cohesin and dockerin domains from Clostridium thermocellum". Biochemical Journal 424 (2009): 375-384. 10.1042/BJ20091152 Montanier, Cedric; Money, Victoria A.; Pires, Virginia M. R.; Flint, James E.; Pinheiro, Benedita A.; Goyal, Arun; Prates, Jose A. M.; et al. "The Active Site of a Carbohydrate Esterase Displays Divergent Catalytic and Noncatalytic Binding Functions". Plos Biology 7 3 (2009): 687-697. 10.1371/journal.pbio.1000071 Najmudin, Shabir; Pinheiro, Benedita A.; Romao, Maria J.; Prates, Jose A. M.; Fontes, Carlos M. G. A.. "Purification, crystallization and crystallographic analysis of Clostridium thermocellum endo-1,4-beta-D-xylanase 10B in complex with xylohexaose". Acta Crystallographica Section F-Structural Biology and Crystallization Communications 64 (2008): 715-718. 10.1107/S1744309108019696 Pinheiro, Benedita A.; Proctor, Mark R.; Martinez-Fleites, Carlos; Prates, Jose A. M.; Money, Victoria A.; Davies, Gideon J.; Bayer, Edward A.; et al. "The Clostridium cellulolyticum dockerin displays a dual binding mode for its cohesin partner". Journal of Biological Chemistry 283 26 (2008): 18422-18430. 10.1074/jbc.M801533200

Other

Other output

Resistance to Aminoglycosides. Glycomics and the Link to the Human Gut Microbiome. 2019. Viviana Gomes Correia; Pinheiro, Benedita; Carvalho, Ana Luísa; Palma, Angelina S.. http://dx.doi.org/10.1002/9781119282549.ch1.
10.1002/9781119282549.ch1
The Structural Basis of the Recognition of Di-glucosylated N-glycans by the ER Lectin Malectin. Glycobiology. 2013. Ana Luísa Carvalho; Maria João Romão; Palma, Angelina S.; Pinheiro, Benedita; Liu, Yan; Takeda, Yoichi; Chai, Wengang; et al. https://novaresearch.unl.pt/en/publications/a3f0fd44-8cef-4765-a321-f549d44a6fa4.

Activities

Oral presentation

	Presentation title	Event name Host (Event location)
2023/06/16	Molecular Characterization of O-glycan selective proteins from human commensal bacteria	15th Jenner Glycobiology and Medicine Symposium Institute for Research and Innovation in Health (i3S) (Porto, Portugal)
2023/01/17	Mapping Molecular Recognition of beta-1,3-1,4-Glucans by a Surface Glycan-Binding Protein from the Human Gut Symbiont Bacteroides ovatus	14th International meeting of the Portuguese Carbohydrate Group (GLUPOR 14) FCT-NOVA (Caparica, Portugal)
2020/12/16	Exploring the human microbiome for novel glyco-selective proteins acting on dietary and host glycans	bioSeminars@UCIBIO UCIBIO (Caparica)
2019	Structure of a novel CBM provides further insight into the bacterial degradation of complex pectic polysaccharides	13th International Meeting of the Portuguese Carbohydrate Group (Glupor 13) i3S – Instituto de Investigação e Inovação em Saúde, Universidade do Porto (Porto, Portugal)
2017	Structural Basis for the Highly Selective Recognition of Di-Glucosylated N-glycans by Human Malectin in the Endoplasmic Reticulum	Glupor12 - 12th International Meeting of Portuguese Carbohydrate Group Universidade Aveiro (Aveiro, Portugal)
2015	A multi-disciplinary study of the cellulosome assembly.	6th European Conference Chemistry in the Life Sciences REQUIMTE-FCT-NOVA (Lisbon, Portugal)
2011	Cohesin-dockerin interactions are determinant for efficient cellulose degradation in cellulolyticum bacteria	9th Carbohydrate Bioengineering Meeting CIISA - FMV/UTL (Lisbon, Portugal)

Supervision

	Thesis Title Role	Degree Subject (Type) Institution / Organization
2023/02 - 2023/06	Discovery and Characterization of novel GlycO-selective mucinases Supervisor	Bioquímica (Degree) Applied Molecular Biosciences Unit (UCIBIO), Portugal Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2023/02 - 2023/06	Development Cancer Glycan Binding proteins Supervisor	Bioquímica (Degree) Applied Molecular Biosciences Unit (UCIBIO), Portugal Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2022/09/01 - 2022/09/30	Descoberta e caracterização de novas o-enzimas do microbioma para análise da glicosilação tipo mucina Supervisor	Iniciação à Investigação em Ciências Biomoleculares (Scientific initiation) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2022/09/01 - 2022/09/30	Desenvolvimento de módulos de deteção de glicosilação aberrante em células cancerígenas Co-supervisor	Iniciação à Investigação em Ciências Biomoleculares (Scientific initiation) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2022 - 2022	Glycan Binding Proteins for Cancer Glycan Recognition Co-supervisor	Erasmus research practical course (Scientific initiation) Applied Molecular Biosciences Unit (UCIBIO), Portugal Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2021 - 2021	Structural and functional characterization of the human malectin glycan-binding pocket Supervisor	Bioquímica para a Saúde (Master) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2019 - 2021	Structural and functional characterization of the human malectin glycan-binding pocket Supervisor	Mestrado Bioquímica para saúde (Master) Applied Molecular Biosciences Unit (UCIBIO), Portugal Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2017 - 2017	Assignment of new roles for malectin-like domains to understand their divergent evolution Supervisor	Bioquímica (Master) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2016 - 2017	Assignment of new roles for malectin-like domains to understand their divergent evolution Supervisor	Bioquímica (Master) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal Applied Molecular Biosciences Unit (UCIBIO), Portugal
2015 - 2015	A organização molecular do Celulossoma: caracterização estrutural da proteína CipA e estudo da diversidade das interações coesina-doquerina Supervisor	Bioquímica (Master) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2014 - 2015	Estudos da organização do celulossoma: caracterização estrutural da proteína CipA por Cristalografia de Raios-X e estudo da diversidade das interacções coesina-doquerina utilizando Microarrays Supervisor	Bioquímica (Master) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal Applied Molecular Biosciences Unit (UCIBIO), Portugal
2014 - 2014	MSc Fellow hired under project EXPL/BBB-BEP/0506/2012 Supervisor
2014 - 2014	Microarrays de coesina para decifrar o código de montagem do celulossoma a uma escala proteómica Supervisor	Bioquímica Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2014 - 2014	Malectina: Novo processo de purificação Supervisor	Bioquímica (Degree) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2013 - 2013	Estudo da Interação coesina-doquerina e o seu papel na formação do celulossoma de C. thermocellum Supervisor	Brazilian interchange program “Ciência sem fronteiras”, (Degree) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2012 - 2012	Caracterização bioquímica, por electroforese, de vários módulos proteicos do Celulossoma Supervisor	Bioquímica (Degree) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal

Event organisation

	Event name Type of event (Role)	Institution / Organization
2023/01/16 - 2023/01/18	14th International Meeting of the Portuguese Carbohydrate Group The meeting is dedicated to discussing scientific advances in carbohydrate research by displaying various and cross-disciplinary areas within the field of glycosciences, with a focus on basic and applied applications. The meeting also provides a unique opportunity for the carbohydrate national and international community to form new collaborations and strengthen existing ones. (2023/01/16 - 2023/01/18) Conference (Member of the Organising Committee)	Applied Molecular Biosciences Unit (UCIBIO), Portugal Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2019 - 2019	Local Organizing Committee (Volunteer) of the European Union Science Olympiad 2019 (EUSO 2019), FCT-NOVA, Caparica, Portugal. (https://www.euso2019.pt/) (2019 - 2019)	Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2018 - 2018	UCIBIO Annual Meeting (2018 - 2018) (2018 - 2018) Meeting (Member of the Organising Committee)	Applied Molecular Biosciences Unit (UCIBIO), Portugal

Event participation

	Activity description Type of event	Event name Institution / Organization
2020/11/23 - 2020/11/27	EMBL-EBI Structural Bioinformatics course Workshop	EMBL-EBI Structural Bioinformatics
2017/07/06 - 2017/07/14	EMBO practical course on HTP techniques for protein production and crystallization. Workshop	High-throughput protein production and crystallization, EMBO Course University of Oxford Research Complex at Harwell, United Kingdom
2016/05/29 - 2016/06/04	Course on theoretical principles of crystallography Workshop	3rd International Summer School of Crystallography Center for Free-Electron Laser Science, Germany
2014/05/26 - 2014/05/30	EMBO course on GLycobiology and Glycochemistry Workshop	Glycobiology and glycochemistry: Applications to human health and disease NOVA Medical School - CEDOC, Portugal
2007/03/26 - 2007/03/30	Course on Protein Crystallography Workshop	Protein Crystallography Applied Molecular Biosciences Unit (UCIBIO), Portugal Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2006/03/11 - 2006/03/15	Bioinformatic course sequence analysis Workshop	SAHPC06: Sequence Analysis using High Performance Computing Instituto Gulbenkian de Ciência, Portugal

Jury of academic degree

	Topic Role	Candidate name (Type of degree) Institution / Organization
2023/12/14	The impact of defective host cell glycosylation in bacterial infection (Thesis) Main arguer	Patrícia Alexandra Nunes Mexia (Master) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica António Xavier, Portugal
2023/07	The role of microbiota on the rate of horizontal gene transfer events in a mouse model of Inflammatory Bowel Disease (Thesis) Main arguer	Maria Leonor Silva (Other) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2022/07	Isolamento e caracterização Bioquímica da Peroxidase da Escherichia coli (Thesis) Main arguer	Catarina Rosado Correia (Other) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2021/07	Caracterização da interação do domínio C-terminal da endosina Twort com o peptidoglicano de S. aureus (Thesis) Main arguer	Francisco Seco (Other) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal

Association member

	Society Organization name	Role
2015 - Current	Sociedade Portuguesa de Química	Membro
2015 - Current	Portuguese Carbohydrate Group	Membro

Consulting

	Activity description	Institution / Organization
2023 - 2023	Analysis of the Pequi exocarp total extract for lectin activity using glycan microarrays.	Universidade de Lisboa Faculdade de Farmácia, Portugal Universidade de Lisboa Instituto Superior de Agronomia, Portugal
2021 - 2023	Pre-clinical characterization of antibodies for a clinical trial using carbohydrate microarrays for CellmAbs biopharmaceuticals.

Course / Discipline taught

	Academic session	Degree Subject (Type)	Institution / Organization
2020 - Current	Practical classes of Metabolism and Regulation curricular unit, a total of 28 hours	(Licenciatura)	Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2023/09/25 - 2023/09/28	"Hands-on: Protein crystallization" module of the GlycoTwinning Summer School "Scientific Hands-on course in protein/glycan interactions: a structural perspective. NMR, X-ray crystallography, cryoEM and molecular modelling."	(Pós-Graduação)	Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2020/07/27 - 2020/07/31	UCIBIO Summer School, Programa “Ciência no Verão” FCT-MCTES, Lesson on MST and ITC biophysical techniques, 29 July, FCT-NOVA	(Mestrado)	Unidade de Ciências Biomoleculares Aplicadas, Portugal
2019 - 2019	Practical classes of Metabolism and Regulation curricular unit, a total of 25 hours	Biologia Celular e Molecular; Química Aplicada (Licenciatura)	Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2015/07/13 - 2015/07/19	International hands-on Course ISBio2015: Integrative Structural Biology tools for the study of protein-ligand interactions. INSTRUCT/COST supported training course. (co-organization and teaching; supervisor of the Isothermal Titration Calorimetry module).	(Outros)	Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2014/10/06 - 2014/10/12	Course ISBio2014: Integrative Structural Biology tools for the study of protein-ligand interactions. INSTRUCT/COST supported training course (co-organization and teaching in the Isothermal titration Calorimetry module).	(Outros)	Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2014 - 2014	Practical crystallization classes of the curricular unit Structural Biochemistry	Bioquímica (Mestrado integrado)	Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2014 - 2014	Practical crystallization classes of the curricular unit Fundaments of Structural Biochemistry	Bioquímica (Licenciatura)	Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2010 - 2014	Practical Classes of Metabolism and Regulation curricular unit, 15 hours per year	Bioquímica (Licenciatura)	Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal

Mentoring / Tutoring

	Topic	Student name
2024/01 - Current	Molecular Biology/Biochemistry/Carbohydrate Microarrays	Ana Luísa Da Silva Ribeiro Benavente
2022 - Current	Molecular Biology	Filipa Engrola
2017 - Current	Molecular Biology / Protein Biochemistry and Crystallography	Filipa Trovão
2015 - Current	Molecular Biology / Protein Biochemistry and Crystallography	Raquel Costa
2021 - 2024	Biochemistry / Protein microarrays	Nuno Lopes
2016 - 2019	Molecular Biology / Protein Biochemistry and Crystallography	Viviana Correia
2016 - 2019	Molecular Biology / Protein Biochemistry and Crystallography	Diana Ribeiro

Distinctions

Award

2014	Best Poster Award at the EMBO course "Glycobiology and glycochemistry: Applications to human health and disease" NOVA Medical School - CEDOC, Portugal