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Dr. Mario Del Rosario is currently a postdoctoral researcher in the lab of Prof. Ricardo Henriques and currently affiliated with the Gulbenkian Institute for Molecular Medicine and ITQB NOVA, in Oeiras, Portugal. Mario completed his undergraduate studies at Escuela Superior Politécnica del Litoral (Ecuador), where he studied for a BSc in Biology. He then did a Maters in Research in Biomedical Sciences at the University of Glasgow, in Scotland, where he focused on host-pathogen interactions. He remained in the same lab for his PhD, which he completed in 2019. During this time, he learned and developed super-resolution microscopy tools to study Toxoplasma gondii -host cell interactions, especially, actin dynamics and invasion kinetics. His current work as a postdoc focuses on using artificial intelligence and image analysis to study the biology of phototoxicity, leveraging cell health cues to assess photodamage and cellular stress. Additionally, he uses expansion and super-resolution microscopy to study pathogens.
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Identification

Personal identification

Full name
Mario Del Rosario

Citation names

  • Del Rosario, Mario
  • Mario Igor Del Rosario Minina

Author identifiers

Ciência ID
CF1C-E1E2-6217
ORCID iD
0000-0002-0430-1463
Google Scholar ID
oR_6MuoAAAAJ
Researcher Id
ABA-5811-2021

Email addresses

  • mario.delrosario@itqb.unl.pt (Professional)
  • mario.delrosario@itqb.unl.pt (Personal)

Telephones

Mobile phone
  • 912660162 (Personal)

Addresses

  • Av. da República, 2780-157, OEIRAS, LISBOA, Portugal (Professional)
  • Rua Antero de Quental 11, 5E, 2780-170, Oeiras, Lisbon, Portugal (Personal)

Knowledge fields

  • Natural sciences - Biological Sciences - Microbiology
  • Engineering and Technology
  • Engineering and Technology - Medical Engineering

Languages

Language Speaking Reading Writing Listening Peer-review
Spanish; Castilian (Mother tongue)
English Proficiency (C2) Proficiency (C2) Proficiency (C2) Proficiency (C2)
Russian Elementary (A2) Beginner (A1) Beginner (A1) Elementary (A2)
Portuguese Intermediate (B1) Advanced (C1) Upper intermediate (B2) Upper intermediate (B2)
Education
Degree Classification
2015/05/08 - 2019/10/09
Concluded
 PhD in Infection and Immunobiology (Doktor (PhD))
Major in Molecular Parasitology
University of Glasgow, United Kingdom
"The role of actin dynamics during Toxoplasma gondii invasion" (THESIS/DISSERTATION)
 PhDs are not graded in the UK
2013/09/16 - 2014/09/16
Concluded
 MRes in Biomedical Sciences (Merit) (Master)
Major in Biomedical Sciences
University of Glasgow, United Kingdom
"Toxoplasma gondii: differences in invasion between macrophages and epithelial cells." (THESIS/DISSERTATION)
 14
2007/04/04 - 2012/02/04
Concluded
 Bachelor of Science (BSc), Biology (Bachelor)
Major in Biology
Escuela Superior Politécnica del Litoral, Ecuador
"Presence of Antimicrobial Activity in the Mucus of Chame Fish (Dormitator latifrons)" (THESIS/DISSERTATION)
 14.825
Affiliation

Science

Category
Host institution 		Employer
2024/10/01 - Current Postdoc (Research) Universidade Nova de Lisboa Molecular Structural and Cellular Microbiology, Portugal
Universidade Nova de Lisboa Molecular Structural and Cellular Microbiology, Portugal
(...)
2020/12/01 - Current Postdoc (Research) Gulbenkian Institute for Molecular Medicine, Portugal
Gulbenkian Institute for Molecular Medicine, Portugal
(...)
2019/05/01 - 2019/09/28 Postdoc (Research) University of Glasgow, United Kingdom
University of Glasgow, United Kingdom
Projects

Contract

Designation Funders
2023/01/01 - Current Artificial Intelligence Guided 4D Super Resolution Microscopy
ERC-CoG-101001332-SelfDriving4DSR
Post-doc
Instituto Gulbenkian de Ciência, Portugal

Gulbenkian Institute for Molecular Medicine, Portugal
Ongoing

Other

Designation Funders
2015/06/05 - 2019/09/22 Apicomplexan F-actin is required for efficient nuclear entry during host cell invasion
CZ5Y8-2021-0295-O
PhD Student Fellow
University of Glasgow Wellcome Centre for Molecular Parasitology, United Kingdom

SENESCYT, Secretaría de Educación Superior, Ciencia, Tecnología e Innovación, Ecuador
SENESCYT, Secretaría de Educación Superior, Ciencia, Tecnología e Innovación
Concluded
Outputs

Publications

Book
  1. Toxoplasma gondii: Biology and Role in Health and Disease. Nova Science Publishers. 2021.
    10.52305/zqkt5678
Conference poster
  1. Del Rosario, Mario; Gómez-de-Mariscal, Estibaliz; Leonor Morgado; Guillaume Jacquemet; Pedro Matos Pereira; Ricardo Henriques. "Discovering the temporal footprint of phototoxicity". Paper presented in Single Molecule Localization Microscopy Symposium 2023, 2023.
  2. Del Rosario, Mario; Gómez-de-Mariscal, Estibaliz; Leonor Morgado; Pedro Matos Pereira; Ricardo Henriques. "Assessing the temporal footprint of phototoxicity". Paper presented in Imaging Cell Dynamics 2023, 2023.
  3. Del Rosario, Mario; Heil, Hannah; Leandro Lemgruber; Henriques, Ricardo. "Atlas Parasitus: Building A 3d Toxoplasma Gondii Cellular Components Map To Study Organelle Reorganisation During Cell Division.". Paper presented in Focus on Microscopy 2022, 2022.
  4. Del Rosario, Mario; Javier Periz; Jake Baum; Isabelle Tardieux; Markus Meissner. "Apicomplexan F-actin is required for efficient nuclear entry during host cell invasion". Paper presented in XXIX Annual Molecular Parasitology Meeting, 2018.
  5. Del Rosario, Mario. "Apicomplexan F-actin is required for efficient nuclear entry during host cell invasion". Paper presented in BioMalPar XIII: Biology and Pathology of the Malaria Parasite, 2017.
Journal article
  1. Dominik Kylies; Heil, Hannah; Vesga, Arturo; Del Rosario, Mario; Maria Schwerk; Malte Kuehl; Milagros N. Wong; Victor G. Puelles; Ricardo Henriques. "Expansion and fluctuations-enhanced microscopy for nanoscale molecular profiling of cells and tissues". Nature Protocols (2025):
    Under revision
  2. Del Rosario, Mario; Gómez-de-Mariscal, Estibaliz; Leonor Morgado; Raquel Portela; Guillaume Jacquemet; Pedro Pereira; Ricardo Henriques. "PhotoFiTT: A Quantitative Framework for Assessing Phototoxicity in Live-Cell Microscopy Experiments". Nature Communications (2025):
    Under revision
  3. Estibaliz Gómez-de-Mariscal; Mario Del Rosario; Joanna W. Pylvänäinen; Guillaume Jacquemet; Ricardo Henriques. "Harnessing artificial intelligence to reduce phototoxicity in live imaging". Journal of Cell Science (2024): https://doi.org/10.1242/jcs.261545.
    10.1242/jcs.261545
  4. Artemis G. Korovesi; Leonor Morgado; Fumasoni, Marco; Henriques, Ricardo; Heil, Hannah S; Del Rosario, Mario. Corresponding author: Del Rosario, Mario. "Expansion Microscopy on Saccharomyces cerevisiae". microPublications (2022): https://www.micropublication.org/journals/biology/micropub-biology-000566.
    10.17912/MICROPUB.BIOLOGY.000566
  5. Mario Del Rosario; Hannah S. Heil; Afonso Mendes; Vittorio Saggiomo; Ricardo Henriques. "The Field Guide to 3D Printing in Optical Microscopy for Life Sciences". Advanced Biology (2021): https://doi.org/10.1002/adbi.202100994.
    10.1002/adbi.202100994
  6. Mario Del Rosario; Javier Periz; Georgios Pavlou; Oliver Lyth; Fernanda Latorre-Barragan; Sujaan Das; Gurman S Pall; et al. "Apicomplexan F-actin is required for efficient nuclear entry during host cell invasion". EMBO reports 20 12 (2019): https://doi.org/10.15252/embr.201948896.
    10.15252/embr.201948896
  7. Javier Periz; Mario Del Rosario; Alexandra McStea; Simon Gras; Colin Loney; Lin Wang; Marisa L. Martin-Fernandez; Markus Meissner. "A highly dynamic F-actin network regulates transport and recycling of micronemes in Toxoplasma gondii vacuoles". Nature Communications 10 1 (2019): https://doi.org/10.1038/s41467-019-12136-2.
    10.1038/s41467-019-12136-2
  8. Rosario, Mario; Johannes Felix Stortz; Mario Del Rosario; Mirko Singer; Jonathan M Wilkes; Markus Meissner; Sujaan Das. "Formin-2 drives polymerisation of actin filaments enabling segregation of apicoplasts and cytokinesis in Plasmodium falciparum". eLife 8 (2019): http://dx.doi.org/10.7554/elife.49030.
    10.7554/elife.49030
  9. Periz J; Whitelaw J; Harding C; Gras S; Del Rosario Minina MI; Latorre-Barragan F; Lemgruber L; et al. "Toxoplasma gondii F-actin forms an extensive filamentous network required for material exchange and parasite maturation.". eLife (2017): http://europepmc.org/abstract/med/28322189.
    10.7554/eLife.24119
  10. Del Rosario, M.; De La Torre, H.; Reyes, D.; Noboa, A.; Salazar, L.; Marcillo, E.; Landívar, J.; Muñoz, M.. "Presence of antimicrobial activity in the mucus of chame fish (Dormitator latifrons)". Journal of Pure and Applied Microbiology 66 4 (2012): 1615-1622. http://www.scopus.com/inward/record.url?eid=2-s2.0-84874004098&partnerID=MN8TOARS.
Preprint
  1. Ricardo Henriques; Mario del Rosario; Estibaliz Gómez-de-Mariscal; Leonor Morgado; Raquel Portela; Guillaume Jacquemet; Pedro Pereira. "PhotoFiTT: A Quantitative Framework for Assessing Phototoxicity in Live-Cell Microscopy Experiments". 2024. https://doi.org/10.21203/rs.3.rs-4809905/v1.
    10.21203/rs.3.rs-4809905/v1
  2. Mario Del Rosario. "Harnessing Artificial Intelligence To Reduce Phototoxicity in Live Imaging". 2023. https://arxiv.org/abs/2308.04387.
  3. "The Field Guide to 3D Printing in Microscopy". 2021. http://dx.doi.org/10.20944/preprints202105.0352.v1.
    10.20944/preprints202105.0352.v1
Thesis / Dissertation
  1. "Apicomplexan F-actin is required for efficient nuclear entry during host cell invasion". PhD, University of Glasgow, 2019. http://theses.gla.ac.uk/id/eprint/75066.
    10.5525/gla.thesis.75066

Other

Other output
  1. Apicomplexan F-actin is required for efficient nuclear entry during host cell invasion. 2019. Del Rosario, M.; Periz, J.; Pavlou, G.; Lyth, O.; Latorre-Barragan, F.; Das, S.; Pall, G.S.; et al. http://www.scopus.com/inward/record.url?eid=2-s2.0-85095637915&partnerID=MN8TOARS.
    10.1101/646463
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2024/11/10 PhotoFiTT: A Framework for Assessing Phototoxicity in Live-Cell Microscopy Experiments Spanish & Portuguese Advanced Optical Microscopy Meeting - SPAOM24
Red Española de Microscopía Óptica Avanzada (Toledo, Spain)
2024/08/30 PhotoFiTT: A Framework for Assessing Phototoxicity in Live-Cell Microscopy Experiments Single Molecule Localization Microscopy Symposium 2024
Universidad NOVA (Lisbon, Portugal)
2021/09/29 Apicomplexan F-actin is required for efficient nuclear entry during host cell invasion Microscopy at the Frontiers of Science
Sociedade Portuguesa de Microscopia (Braga, Portugal)
2020/09 Apicomplexan F-actin is required for efficient nuclear entry during host cell invasion A bata quitada - Science Outreach Event in Ecuador
(Guayaquil, Ecuador)
2018/05/06 Apicomplexan F-actin is required for efficient nuclear entry during host cell invasion BioMalPar XIV: Biology and Pathology of the Malaria Parasite
European Molecular Biology Laboratory (Heidelberg, Germany)

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2022/01/11 - 2026/01/11 Prediction and Super-Resolution Observation of Structural Dynamics
Co-supervisor
 Integrative Biology and Biomedicine (PhD)
Instituto Gulbenkian de Ciência, Portugal

Event organisation

Event name
Type of event (Role) 		Institution / Organization
2024/04/04 - 2025/10/10 Main organiser of the "Democratizing microscopy for imaging in Latin America: imaging across scales" workshop in Ecuador, South America (2024/04/04 - 2025/10/10)
Workshop (Co-organisor)
 The Company of Biologists Ltd, United Kingdom
2024/10/02 - 2024/10/04 Trainer and mentor in Expansion Microscopy techniques at the "Basics of Advanced Microscopy" workshop (2024/10/02 - 2024/10/04)
Workshop (Other)
 Instituto Gulbenkian de Ciência, Portugal
2024/08/20 - 2024/08/30 Volunteer at Single Molecule Localization Microscopy Symposium 2024 (2024/08/28 - 2024/08/30)
Conference (Other)
 Instituto Gulbenkian de Ciência, Portugal

Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica António Xavier, Portugal
2023/09/18 - 2023/09/29 Trainer in the workshop "Fundamentals of light microscopy and image processing". Teach and trained workshop attendees on expansion microscopy principles and techniques (2023/09/18 - 2023/09/29)
Workshop (Other)
 Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica António Xavier, Portugal
2023/03/31 - 2023/03/31 Trainer in the Abbelight workshop "Beyond Diffraction: Trends in Nanoscopy". Lead the workshop on Expansion Microscopy techniques that includes sample preparation, image acquisition pipelines and the subsequent image analysis. (2023/03/31 - 2023/03/31)
Workshop (Other)
 Instituto Gulbenkian de Ciência, Portugal

Abbelight, France

Event participation

Activity description
Type of event 		Event name
Institution / Organization
2024/11/20 - 2024/11/22 Presenter at the phototoxicity and live-cell workshop. This includes live demostrations of different deep learning tools including ZeroCostDL4Mic and PhotoFiTT at SPAOM24
Workshop
 Spanish & Portuguese Advanced Optical Microscopy Meeting - SPAOM24
Portuguese Platform of BioImaging, Portugal

Interview (newspaper / magazine)

Activity description Newspaper / Forum
2024/09/17 Featured on the postdoctoral excellence showcase during the postdoc appreciation week The Company of Biologist
2023/08/22 Latin American Microscopist - An interview for FocalPlane FocalPlane
2019/10/14 Interview about my PhD work for the University of Glasgow Institute Research Uncovers Novel Invasion Mechanism For Toxoplasma

Mentoring / Tutoring

Topic Student name
2021/10/04 - 2021/10/22 Expansion microscopy on Saccharomyces cerevisiae Artemis Korovesi
2021/10/04 - 2021/10/22 Expansion Microscopy on CHO and yeast cells Leonor Morgado
Distinctions

Award

2015 Scholarship for University of Excellence 2015 – 2019
SENESCYT, Secretaría de Educación Superior, Ciencia, Tecnología e Innovación, Ecuador
2013 Scholarship for University of Excellence 2013 – 2014
SENESCYT, Secretaría de Educación Superior, Ciencia, Tecnología e Innovación, Ecuador

Other distinction

2024 Travel Grant for SPAOM24
Portuguese Platform of BioImaging, Portugal
2019 An image of my work was selected as the cover for the EMBO Reports Journal, volume 20, 12th edition in December 2019.