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MJS earned her PhD in 2016 (IST, Lisbon), specializing in membrane biophysics. Her research focused on unravelling the organization of PIP2 phosphoinositol in both membrane mimetics and live cell membranes. During her PhD, she spent two months at King's College London (UK), conducting time-resolved fluorescence anisotropy imaging, a technique she later implemented in her home laboratory. Post-PhD, she ventured to IIT in Genoa (Italy) for her initial PostDoc (2016-2017), employing STED-based approaches to visualize nuclear structures in acute promyelocytic leukaemia. Subsequently, she joined the Heyrovský Institute in Prague (Czech Republic) delving into single-molecule fluorescence microscopy and spectroscopy to decipher the protective role of gangliosides against membrane-mediated oligomerization of amyloidogenic proteins implicated in neurodegenerative diseases. In 2019, MJS received both the CEECind (FCT) and a Marie Curie fellowship to investigate the molecular determinants of healthy human aging. Returning to Lisbon in June 2020, she joined iMM where she currently explores lipid-lamin interactions in the nuclear compartment and their impact on nuclear structural integrity during premature and physiological aging. This includes working with both model systems and primary cells, and implementing different approaches in the host lab, such as nuclei isolation, lamin purification and lipidomics. With more than 10 years of experience, MJS now possesses advanced expertise in quantitative biophysical approaches, including several optical microscopy techniques (confocal, FRET, FLIM, FCS, FAIM, STED) and steady-state/time-resolved fluorescence spectroscopy. Her skills extend, for example, from vesicle preparation and lipid biochemistry to live cell handling. She plays an active role in mentoring students and fostering national and international collaborations. Additionally, she has been contributing to the Young Researchers and Innovators committee within COST Action EpiLipidNET (focused on lipidomics analysis) since 2021. She has authored numerous publications in esteemed journals, such as Nature Communications, Chemical Reviews, Cellular and Molecular Life Sciences, Journal of Physical Chemistry Letters and Frontiers in Cell and Developmental Biology, often serving as the first and/or corresponding author.
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Identification

Personal identification

Full name
Maria João Martins Sarmento

Citation names

  • Sarmento, Maria J.

Author identifiers

Ciência ID
DC17-AA56-FFDE
ORCID iD
0000-0003-1765-4724
Researcher Id
O-4818-2018
Scopus Author Id
56083323900

Email addresses

  • maria.sarmento@medicina.ulisboa.pt (Professional)

Websites

Knowledge fields

  • Natural sciences - Biological Sciences - Biophysics
  • Natural sciences - Biological Sciences - Biochemistry

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese (Mother tongue)
English Advanced (C1) Proficiency (C2) Advanced (C1) Advanced (C1) Advanced (C1)
Education
Degree Classification
2012/01 - 2016/04
Concluded
 Química (Doutoramento)
Universidade de Lisboa Instituto Superior Técnico, Portugal
"Lateral organization of PI(4,5)P2 in model membranes and at the plasma membrane of living cells " (THESIS/DISSERTATION)
 Pass with Distinction and Honour
2011
Concluded
 Bioquímica (ramo médica) (Mestrado)
Universidade de Lisboa Faculdade de Ciências, Portugal
"Effect of palmitoylated synaptic proteins and biologically relevant calcium concentrations on the compartmentalization of PI(4,5)P2 in membrane model systems and in the plasma membrane of PC12 cells" (THESIS/DISSERTATION)
 18
2009
Concluded
 Bioquímica (Licenciatura)
Universidade de Lisboa Faculdade de Ciências, Portugal
 16
Affiliation

Science

Category
Host institution 		Employer
2017/05/01 - 2020/05/31 Contracted Researcher (Research) Ústav fyzikální chemie J Heyrovského Akademie ved Ceské republiky, Czech Republic
2016/04/16 - 2017/03/31 Contracted Researcher (Research) Istituto Italiano di Tecnologia, Italy
2012/01 - 2016/04 Researcher (Research) Universidade de Lisboa Centro de Química-Física Molecular, Portugal
2014/11/01 - 2014/12/20 Visiting Researcher (Research) King's College London, United Kingdom
2010/09 - 2011/11 Research Trainee (Research) Universidade de Lisboa Centro de Química-Física Molecular, Portugal
2010/03/01 - 2010/06/30 Research Trainee (Research) Uniwersytet Wroclawski, Poland

Others

Category
Host institution 		Employer
2020/06/01 - Current CEEC Junior Researcher (FCT) Instituto de Medicina Molecular, Portugal
Instituto de Medicina Molecular, Portugal
2021/09 - 2023/08 Marie Sklodowska-Curie Individual Fellowship Instituto de Medicina Molecular, Portugal
2008/09/01 - 2009/07/31 Trainee research grant Universidade de Lisboa / Fundação Amadeu Dias Universidade de Lisboa Centro de Química e Bioquímica, Portugal
Projects

Grant

Designation Funders
2011/02/01 - 2016/04/11 Relationship between PI(4,5)P2 compartmentalization and palmitoylated synaptic proteins in membrane model systems and in the plasma membrane of neuron and non-neuron like cells.
info:eu-repo/grantAgreement/FCT/3599-PPCDT/112067/PT
PhD Student Fellow
Fundação para a Ciência e a Tecnologia
2008 - 2009 Do Design à Síntese de Novos Agentes Anti-Tuberculares
PTDC/QUI/67933/2006
Scientific Initiation Fellow

Contract

Designation Funders
2020/12/01 - Current Exploring lamin-lipid interactions and the loss of nuclear structural integrity as the molecular determinants of human aging
892321
Principal investigator
Instituto de Medicina Molecular, Portugal
 EU Framework Programme for Research and Innovation Marie Sklodowska-Curie Actions
Concluded
2020/06/01 - Current Structural integrity of the nuclear envelope: investigating the molecular determinants of human aging (individual support)
CEECIND/00098/2018
Principal investigator
Instituto de Medicina Molecular, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2021/01/01 - 2025/12/31 Institute of Molecular Medicine João Lobo Antunes
LA/P/0082/2020
Universidade de Lisboa Instituto de Medicina Molecular João Lobo Antunes, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2017/04/01 - 2020/05/31 The role of gangliosides in the development of neurodegenerative amyloidogenic diseases: Molecular insights by advanced fluorescence microscopy
17-03160S
Researcher
Ústav fyzikální chemie J Heyrovského Akademie ved Ceské republiky, Czech Republic
Concluded
2016/04/17 - 2017/03/31 Super-resolution imaging of the organization of transcription and replication during oncogene-induced replicative stress
TRIDEO
Researcher
2012/03/01 - 2015/08/31 Interaction of bile acids with lipid membranes: creating a shield or bursting the bubble.
PTDC/QUI-BIQ/119494/2010
Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal

Universidade de Lisboa Centro de Química-Física Molecular, Portugal

Universidade de Lisboa Faculdade de Farmácia, Portugal
Fundação para a Ciência e a Tecnologia
Concluded

Other

Designation Funders
2020 - Current PanEuropean Network in Lipidomics and EpiLipidomics (EpiLipidNET)
CA19105
Young Researchers & Innovators committee
European Cooperation in Science and Technology
Ongoing
2024/01/01 - 2026/12/31 Nano-coatings for prosthetics based on microbial phenazines conjugated with silver nanostars
CI23-10134
Researcher
Universidade de Lisboa Instituto de Medicina Molecular João Lobo Antunes, Portugal
 Fundação 'la Caixa'
Ongoing
2023/10 - 2025/10 COLOR2COAT: Nano-coatings for prosthetics based on microbial phenazines conjugated with silver nanostars (CI23-10134)
CI23-10134
Researcher
Universidade de Lisboa Instituto de Medicina Molecular João Lobo Antunes, Portugal
Ongoing
2024/01/01 - 2024/12/31 ImPlat: A single-cell platform to validate material scaffolds and drugs for the enhanced osteointegration of biomedical implants
ImPlat
Researcher
Universidade de Lisboa Instituto de Medicina Molecular João Lobo Antunes, Portugal
 Plano de Recuperação e Resiliência (PRR) do Centro de Tecnologia e Inovação (CTI) do iMM
Ongoing
2023/03/10 - 2024/09/09 Amp-NStar: AMP-AgNP conjugates: the mutual reinforcement of antibacterial activity
2022.01991.PTDC
10.54499/2022.01991.PTDC
Researcher
Universidade de Lisboa Instituto de Medicina Molecular João Lobo Antunes, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2023/02/01 - 2024/07/31 Biophysical studies of flavivirus capsid protein interactions with key biological targets to achieve its future inhibition
2022.02763.PTDC
10.54499/2022.02763.PTDC
Researcher
Universidade de Lisboa Instituto de Medicina Molecular João Lobo Antunes, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
Outputs

Publications

Book chapter
  1. Maria J. Sarmento; Fábio Fernandes. "Choosing the Right Fluorescent Probe". In Fluorescence Microscopy and Spectroscopy in Biology. Springer International Publishing, 2022.
    Published • 10.1007/4243_2022_30
Conference abstract
  1. Sarmento, Maria J.; Kohutova, Petra; Carvalho, Filomena A.; Hof, Martin; Santos, Nuno C.. "Deciphering the role of nuclear envelope lipids in human healthy aging". Paper presented in EBSA Congress, Stockholm, 2023.
    Published
  2. Hof, Martin; Sarmento, Maria J.; Owen, Michael C.; Ricardo, Joana C.; Chmelová, Barbora; Davidovic, David; Mikhalyov, Ilya; et al. ""Why do gangliosides form nanodomains": An old question answered by combining Monte-Carlo FRET with MD Simulations". Paper presented in 17th MAF, Gothenburg, 2023.
  3. Oneto, M.; Scipioni, L.; Sarmento, Maria J.; Cainero, I.; Cerutti, E.; Pelicci, S.; Furia, L.; et al. "Nanoscale distribution of nuclear sites analyzed by superresolution STED image cross-correlation spectroscopy". Paper presented in 64th Annual Meeting of the Biophysical Society, San Diego, California, 2020.
    Published
  4. Lanzanò, L.; Oneto, M.; Cainero, Isotta; Pelicci, S.; Sarmento, Maria J.; Scipioni, L.; Faretta, M.; et al. "Chromatin alterations in a model of oncogene activation studied by advanced fluorescence microscopy". Paper presented in 63rd Annual Meeting of the Biophysical Society, Baltimore, 2019.
    Published • 10.1016/j.bpj.2018.11.1515
  5. Lanzanò, L.; Sarmento, Maria J.; Scipioni, L.; Oneto, M.; Pelicci, S.; Di Bona, M.; Pesce, L.; et al. "SPLIT-STED imaging of nuclear structures". Paper presented in Biophysical Society 62nd Annual Meeting, San Francisco, 2018.
    Published • https://doi.org/10.1016/j.bpj.2017.11.1940
  6. Sarmento, Maria J.; Scipioni, L.; Di Bona, M.; Faretta, M.; Furia, L.; Dellino, Gaetano I.; Pelicci, Pier G.; et al. "Spatial organization of nuclear structures by dual color super-resolution microscopy". Paper presented in Biophysical Society 61st Annual Meeting, New Orleans, 2017.
    Published • https://doi.org/10.1016/j.bpj.2016.11.1696
  7. Lanzanò, L.; Di Bona, M.; Scipioni, L.; Sarmento, Maria J.; Gratton, E.; Vicidomini, G.; Diaspro, A.. "Heterogeneity of the nuclear environment investigated by super-resolution microscopy and fluorescence correlation spectroscopy". Paper presented in Biophysical Sociey 61st Annual Meeting, New Orleans, 2017.
    Published • DOI:https://doi.org/10.1016/j.bpj.2016.11.783
  8. Scipioni, L.; Di Bona, M.; Sarmento, Maria J.; Vicidomini, G.; Diaspro, A.; Lanzanò, L.. "Quantitative mapping of intranuclear diffusion in living cells by phasor analysis of local RICS". Paper presented in Biophysical Society 61st Annual Meeting, New Orleans, 2017.
    Published • https://doi.org/10.1016/j.bpj.2016.11.1601
  9. Di Bona, M.; Scipioni, L.; Sarmento, Maria J.; Vicidomini, G.; Diaspro, A.; Lanzanò, L.. "Chromatin accessibility studied by slow scan FCS in the eukaryotic nucleus". Paper presented in Biophysical Society 61st Annual Meeting, New Orleans, 2017.
    Published • https://doi.org/10.1016/j.bpj.2016.11.1195
  10. Sarmento, Maria J.; Coutinho, A.; Prieto, M.; Fernandes, F.. "Analysis of PI(4,5)P2 lateral organization at the plasma membrane of living cells through FRET". Paper presented in Biophysical Society 59th Annual Meeting, 2015.
    Published • https://doi.org/10.1016/j.bpj.2014.11.1873
  11. Sarmento, Maria J.; Coutinho, A.; Fedorov, A.; Prieto, M.; Fernandes, F.. "Physiological calcium concentrations induce PI(4,5)P2 clustering: PI(4,5)P2 as a lipidic calcium sensor". Paper presented in Biophysical Society 57th Annual Meeting, Philadelphia, 2013.
    Published • https://doi.org/10.1016/j.bpj.2012.11.2069
  12. Sarmento, Maria J.; Coutinho, A.; Fedorov, A.; Prieto, M.; Fernandes, F.. "PI(4,5)P2 acts as a lipid cancium sensor in the presence pf physiological calcium concentrations". Paper presented in 9th European Biophysics Congress, Lisboa, 2013.
    Published
  13. Sarmento, Maria J.; Prieto, M.; Fernandes, F.. "High affinity immobilization of giant unilamellar vesicles (GUVs) induces redistribution of lipid domains". Paper presented in Biophysical Society 56th Annual Meeting, San Diego, 2012.
    Published • https://doi.org/10.1016/j.bpj.2011.11.1634
  14. Fernandes, F.; Sarmento, Maria J.; Prieto, M.. "Tethering of lipid membranes reorganizes the distribution of lipid domains". Paper presented in 22nd IUBMB & 37th FEBS Congress, Seville, 2012.
    Published • https://doi.org/10.1111/j.1742-4658.2010.08705.x
  15. Sarmento, Maria J.; Fernandes, F.; Prieto, M.. "Influence of avidin-coated surface for GUV immobilization on lipid domain size and distribution". Paper presented in 8th European Biophysics Congress, Budapeste, 2011.
    Published
  16. Sarmento, Maria J.; Pinto, S.N.; Fernandes, F.; Prieto, M.. "Regulation of PIP2 clustering in neuron-like model cells by synaptic protein palmitoylation". Paper presented in 8th European Biophysics Congress, Budapeste, 2011.
    Published • https://doi.org/10.1007/s00249-011-0734-z
Conference poster
  1. Sarmento, Maria J.; Kohutova, Petra; Carvalho, Filomena A.; Hof, Martin; Santos, Nuno C.. "Deciphering the role of nuclear envelope lipids in human healthy aging". Paper presented in EBSA Congress, 2023.
  2. Sarmento, Maria J.; Kohutova, Petra; Carvalho, Filomena A.; Hof, Martin; Santos, Nuno C.. "Unveiling the role of nuclear envelope lipids in human physiological aging". Paper presented in EMBO Young Scientists' Forum, 2023.
  3. Sarmento, Maria J.; Pais do Amaral, Constança; Santos, Nuno C.. "Deciphering the lipid composition of the human nuclear envelope during healthy aging". Paper presented in Membrane Biophysics Meeting, 2022.
  4. Sarmento, Maria J.; Pais do Amaral, Constança; Santos, Nuno C.. "Deciphering the lipid composition of the human nuclear envelope during healthy aging.". Paper presented in FEBS meeting, 2022.
  5. Patrícia M. Silva; Inês V. Silva; Maria J. Sarmento; Filomena A. Carvalho; Graça Soveral; Nuno C. Santos. "AQP3 and AQP5 silencing promotes changes in cell-cell adhesion and biomechanical properties of pancreatic cancer cells". Paper presented in 20th IUPAB Congress, 45th Annual SBBf Meeting, and 50th Annual SBBq Meeting, 2021.
  6. Dalila Mil-Homens; Maria Martins; José Barbosa; Maria João Sarmento; Rita Pires; Vasco D. B. Bonifácio; Sandra N. Pinto. "Virulence quantification of KPC- and OXA-48-producing Klebsiella pneumoniae isolates in a Galleria mellonella model: Towards a novel therapeutic approach using linear cationic polymers". Paper presented in Galleria Mellonella Workshop, 2021.
  7. Dalila Mil-Homens; Maria Martins; José Barbosa; Maria J. Sarmento; Rita F. Pires; Vasco D. B. Bonifácio; Sandra N. Pinto. "Therapeutic antimicrobial polymers: the polycationic oligoethyleneimine killing performance against KPC- and OXA-48-producing Klebsiella pneumoniae clinical isolates". Paper presented in 31st European Congress of Clinical Microbiology & Infectious Diseases (ECCMID), 2021.
  8. Patrícia M. Silva; Inês V. Silva; Maria J. Sarmento; Filomena A. Carvalho; Graça Soveral; Nuno C. Santos. Corresponding author: Nuno C. Santos. "Effects of aquaporin-3 and aquaporin-5 silencing on pancreatic cancer cell biomechanics and cell-cell adhesion". Paper presented in 7th International Iberian Biophysics congress (Virtual meeting), 2021.
  9. Sarmento, Maria J.; Ricardo, J.C.; Amaro, M.; Hof, M.; Sachl, R.. "Ganglioside nanodomains". Paper presented in Membrane Biophysics Meeting, 2020.
  10. Oneto, M.; Scipioni, L.; Sarmento, Maria J.; Cainero, I.; Cerutti, E.; Pelicci, S.; Furia, L.; et al. "Nanoscale distribution of nuclear sites analyzed by superresolution STED image cross-correlation spectroscopy". Paper presented in 64th Annual Meeting of the Biophysical Society, 2020.
  11. Lanzanò, L.; Oneto, M.; Cainero, I.; Pelicci, S.; Sarmento, Maria J.; Scipioni, L.; Faretta, M.; et al. "Chromatin alterations in a model of oncogene activation studied by advanced fluorescence microscopy". Paper presented in 63rd Annual Meeting of the Biophysical Society, 2019.
  12. Sarmento, Maria J.; Amaro, M.; Hof, M.. "Membrane organization within DIBMALPs.". Paper presented in SMALP User Meeting, 2019.
  13. Borges-Araújo, L.; Monteiro, M.E.; Mil-Homens, D.; Bernardes, N.; Sarmento, Maria J.; Coutinho, A.; Prieto, Manuel; Melo, M.N.; Fernandes, F.. "Impact of Ca2+-dependent PI(4,5)P2 clustering on dynamics of PI(4,5)P2 binding proteins.". Paper presented in Joint 12th EBSA, 10th ICBP-IUPAP Biophysics Congres, 2019.
  14. Sarmento, Maria J.; Amaro, M.; Hof, M.. "Lipid bilayer nanodiscs to study 'in-membrane' features of amyloid peptide oligomerization.". Paper presented in Joint 12th EBSA, 10th ICBP-IUPAP Biophysics Congres, 2019.
  15. Mil-Homens, D.; Martins, M.; Barbosa, J.; Sarmento, Maria J.; Pires, R.; Rogrigues, V.; Bonifácio, V.D.B.. "Virulence quantification of KPC- and OXA-48-producing Klebsiella pneumoniae isolates in a Galleria mellonella model: Towards a novel therapeutic approach using linear cationic polymers". Paper presented in Microbiotec 19, 2019.
  16. Ricardo, J.C.; Sarmento, Maria J.; Amaro, M.; Hof, M.; Sachl, R.. "Aß1-40 peptide influences lipid nanodomains in model membranes.". Paper presented in Biomembrane Days, 2019.
  17. Lanzanò, L.; Sarmento, Maria J.; Scipioni, L.; Oneto, M.; Pelicci, S.; Di Bona, M.; Pesce, L.; et al. "SPLIT-STED imaging of nuclear structures". Paper presented in Biophysical Society 62nd Annual Meeting, 2018.
  18. Borges-Araújo, L.; Monteiro, M.E.; Mil-Homens, D.; Bernardes, N.; Sarmento, Maria J.; Coutinho, A.; Prieto, Manuel; Fernandes, F.. "Impact of Ca2+-dependent PI(4,5)P2 clustering on the properties of PI(4,5)P2 binding proteins.". Paper presented in 6th International Iberian Biophysics Congress – X Iberoamerican congress of Biophysics, 2018.
  19. Di Bona, M.; Scipioni, L.; Sarmento, Maria J.; Vicidomini, G.; Diaspro, A.; Lanzanò, L.. "Chromatin accessibility studied by slow scan FCS in the eukaryotic nucleus". Paper presented in Biophysical Society 61st Annual Meeting, 2017.
  20. Scipioni, L.; Di Bona, M.; Sarmento, Maria J.; Vicidomini, G.; Diaspro, A.; Lanzanò, L.. "Quantitative mapping of intranuclear diffusion in living cells by phasor analysis of local RICS". Paper presented in Biohysical Society 61st Annual Meeting, 2017.
  21. Lanzanò, L.; Di Bona, M.; Scipioni, L.; Sarmento, Maria J.; Gratton, E.; Vicidomini, G.; Diaspro, A.. "Heterogeneity of the nuclear environment investigated by super-resolution microscopy and fluorescence correlation spectroscopy". Paper presented in Biohysical Society 61st Annual Meeting, 2017.
  22. Sarmento, Maria J.; Coutinho, A.; Fedorov, A.; Prieto, M.; Fernandes, F.. "PI(4,5)P2 acts as a lipid cancium sensor in the presence pf physiological calcium concentrations.". Paper presented in 9th EBSA European Biophysics Congress, 2013.
  23. Fernandes, F.; Sarmento, Maria J.; Prieto, M.. "Tethering of lipid membranes reorganizes the distribution of lipid domains". Paper presented in 22nd IUBMB & 37th FEBS Congress, 2012.
  24. Sarmento, Maria J.; Prieto, M.; Fernandes, F.. "High affinity immobilization of giant unilamellar vesicles (GUVs) induces redistribution of lipid domains". Paper presented in Biophysical Society 56th Annual Meeting, 2012.
  25. Sarmento, Maria J.; Fernandes, F.; Prieto, M.. "Regulation of PIP2 clustering in neuron-like model cells by synaptic protein palmitoylation.". Paper presented in 8th European Biophysics Congress, 2011.
  26. Sarmento, Maria J.; Fernandes, F.; Prieto, M.. "Influence of avidin-coated surface for GUV immobilization on lipid domain size and distribution.". Paper presented in 8th European Biophysics Congress, 2011.
Journal article
  1. Maria J. Sarmento; Alicia Llorente; Toni Petan; Denis Khnykin; Iuliana Popa; Matea Nikolac Perkovic; Marcela Konjevod; Morana Jaganjac. "The expanding organelle lipidomes: current knowledge and challenges". Cellular and Molecular Life Sciences 80 8 (2023): http://dx.doi.org/10.1007/s00018-023-04889-3.
    10.1007/s00018-023-04889-3
  2. David Davidovic; Mercedes Kukulka; Maria J. Sarmento; Ilya Mikhalyov; Natalia Gretskaya; Barbora Chmelová; Joana C. Ricardo; et al. "Which moiety drives gangliosides to form nanodomains?". The Journal of Physical Chemistry Letters (2023): https://doi.org/10.1021/acs.jpclett.3c00761.
    10.1021/acs.jpclett.3c00761
  3. Borges-Araújo, Luís; Monteiro, Marina E.; Mil-Homens, Dalila; Bernardes, Nuno; Sarmento, Maria J.; Coutinho, Ana; Prieto, Manuel; Fernandes, Fábio. "Impact of Ca2+-induced PI(4,5)P2 clusters on PH-YFP organization and protein-protein interactions". Biomolecules 12 7 (2022): 912. http://dx.doi.org/10.3390/biom12070912.
    10.3390/biom12070912
  4. Silva, Patrícia M.; da Silva, Inês V.; Sarmento, Maria J.; Silva, Ítala C.; Carvalho, Filomena A.; Soveral, Graça; Santos, Nuno C.. "Aquaporin-3 and aquaporin-5 facilitate migration and cell–cell adhesion in pancreatic cancer by modulating cell biomechanical properties". Cells 11 8 (2022): 1308. http://dx.doi.org/10.3390/cells11081308.
    Published • 10.3390/cells11081308
  5. Sarmento, Maria J.; Owen, Michael C.; Ricardo, Joana C.; Chmelová, Barbora; Davidovic, David; Mikhalyov, Ilya; Gretskaya, Natalia; et al. "The impact of the glycan headgroup on the nanoscopic segregation of gangliosides". Biophysical Journal 120 24 (2021): 5530-5543. http://dx.doi.org/10.1016/j.bpj.2021.11.017.
    10.1016/j.bpj.2021.11.017
  6. Sarmento, Maria J.; Borges-Araújo, Luís; Pinto, Sandra N.; Bernardes, Nuno; Ricardo, Joana C.; Coutinho, Ana; Prieto, Manuel; Fernandes, Fábio. Corresponding author: Sarmento, Maria J.. "Quantitative FRET microscopy reveals a crucial role of cytoskeleton in promoting PI(4,5)P2 confinement". International Journal of Molecular Sciences 22 21 (2021): 11727. http://dx.doi.org/10.3390/ijms222111727.
    10.3390/ijms222111727
  7. Mil-Homens, Dalila; Martins, Maria; Barbosa, José; Serafim, Gabriel; Sarmento, Maria J.; Pires, Rita F.; Rodrigues, Vitória; Bonifácio, Vasco D.B.; Pinto, Sandra N.. "Carbapenem-resistant Klebsiella pneumoniae clinical isolates: In vivo virulence assessment in Galleria mellonella and potential therapeutics by polycationic oligoethyleneimine". Antibiotics 10 1 (2021): 56. http://dx.doi.org/10.3390/antibiotics10010056.
    10.3390/antibiotics10010056
  8. Sarmento, Maria J.; Ricardo, Joana C.; Amaro, Mariana; Sachl, Radek. Corresponding author: Sarmento, Maria J.. "Organization of gangliosides into membrane nanodomains". FEBS Letters 594 22 (2020): 3668-3697. http://dx.doi.org/10.1002/1873-3468.13871.
    10.1002/1873-3468.13871
  9. Sarmento, Maria J.; Hof, Martin; Sachl, Radek. Corresponding author: Sachl, Radek. "Interleaflet coupling of lipid nanodomains - Insights from in vitro systems". Frontiers in Cell and Developmental Biology 8 (2020): http://dx.doi.org/10.3389/fcell.2020.00284.
    10.3389/fcell.2020.00284
  10. Oneto, Michele; Scipioni, Lorenzo; Sarmento, Maria J.; Cainero, Isotta; Pelicci, Simone; Furia, Laura; Pelicci, Pier G.; et al. "Nanoscale distribution of nuclear sites by super-resolved image cross-correlation spectroscopy". Biophysical Journal 117 11 (2019): 2054-2065. http://dx.doi.org/10.1016/j.bpj.2019.10.036.
    Published • 10.1016/j.bpj.2019.10.036
  11. Sarmento, Maria J.; Oneto, M.; Pelicci, S.; Pesce, L.; Scipioni, L.; Faretta, M.; Furia, L.; et al. "Exploiting the tunability of stimulated emission depletion microscopy for super-resolution imaging of nuclear structures". Nature Communications 9 1 (2018): https://doi.org/10.1038/s41467-018-05963-2.
    10.1038/s41467-018-05963-2
  12. Cebecauer, M.; Amaro, M.; Jurkiewicz, P.; Sarmento, Maria J.; Sachl, R.; Cwiklik, L.; Hof, Martin. "Membrane lipid nanodomains". Chemical Reviews 118 23 (2018): 11259-11297. http://dx.doi.org/10.1021/acs.chemrev.8b00322.
    Published • 10.1021/acs.chemrev.8b00322
  13. Sarmento, Maria J.; Coutinho, Ana; Fedorov, Aleksander; Prieto, Manuel; Fernandes, Fábio. Corresponding author: Fernandes, Fábio. "Membrane order is a key regulator of divalent cation-induced clustering of PI(3,5)P2 and PI(4,5)P2". Langmuir 33 43 (2017): 12463-12477. http://dx.doi.org/10.1021/acs.langmuir.7b00666.
    10.1021/acs.langmuir.7b00666
  14. Sarmento, Maria J.; Pinto, S.N.; Coutinho, A.; Prieto, M.; Fernandes, F.. "Accurate quantification of inter-domain partition coefficients in GUVs exhibiting lipid phase coexistence". RSC Advances 6 71 (2016): 66641-66649. http://www.scopus.com/inward/record.url?eid=2-s2.0-84979587338&partnerID=MN8TOARS.
    Published • 10.1039/c6ra13170k
  15. Monteiro, M. E.; Sarmento, Maria J.; Fernandes, Fábio. Corresponding author: Sarmento, Maria J.. "Role of calcium in membrane interactions by PI(4,5)P2-binding proteins". Biochemical Society Transactions 42 5 (2014): 1441-1446. http://dx.doi.org/10.1042/bst20140149.
    Published • 10.1042/bst20140149
  16. Sarmento, Maria J.; Coutinho, A.; Fedorov, Aleksander; Prieto, Manuel; Fernandes, Fabio. "Ca2+ induces PI(4,5)P2 clusters on lipid bilayers at physiological PI(4,5)P2 and Ca2+ concentrations". Biochimica et Biophysica Acta (BBA) - Biomembranes 1838 3 (2014): 822-830. http://dx.doi.org/10.1016/j.bbamem.2013.11.020.
    Published • 10.1016/j.bbamem.2013.11.020
  17. Sarmento, Maria J.; Prieto, M.; Fernandes, F.. "Reorganization of lipid domain distribution in giant unilamellar vesicles upon immobilization with different membrane tethers". Biochimica et Biophysica Acta (BBA) - Biomembranes 1818 11 (2012): 2605-2615. http://dx.doi.org/10.1016/j.bbamem.2012.05.028.
    Published • 10.1016/j.bbamem.2012.05.028
Journal issue
  1. Fábio Fernandes; Maria J. Sarmento. "Protein-lipid interactions and membrane organization (special issue co-editor; open for submission)". Molecules (2022):
    Coeditor
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2024/06/06 Nuclear structural integrity during premature and physiological aging Lipids and Cell Plasticity Symposium
University of Aveiro (Portugal)
2023 Deciphering the role of nuclear envelope lipids in human healthy aging EBSA Congress
EBSA (Stockholm, Sweden)
2022/04/06 Deciphering the lipidome of the human nuclear envelope during healthy age progression (invited) Lipidomics in Health & Disesase
Universidade de Aveiro
2021/10/15 The impact of the glycan headgroup on the nanoscopic segregation of gangliosides (invited) XXI SPB - National Congress of Biochemistry
Portuguese Biochemistry Academy (Évora, Portugal)
2021/10/14 Deciphering the lipidome of the human nuclear envelope during healthy age progression. I PhasAGE International Conference (online)
i3S
2021/09/24 Development of membrane models mimicking the NE in healthy aging (invited; ECI podium) EpiLipidNET COST Action General Meeting
EpiLipidNET COST Action
2021 Virulence quantification of KPC- and OXA-48-producing Klebsiella pneumoniae isolates in a Galleria mellonella model: Towards a novel therapeutic approach using linear cationic polymers Galleria Mellonella Workshop
2019/05 Impact of glycosphingolipids on membrane organization: focus on lipid nanodomains and their influence on peptide-membrane interactions. (invited) FEBS Special Meeting 2019 ¦ Sphingolipid Biology: Sphingolipids in Physiology and Pathology
(Cascais, Portugal)
2018 Impact of Ca2+-dependent PI(4,5)P2 clustering on the properties of PI(4,5)P2 binding proteins. Lisbon Biophysics Network Meeting: From protein dynamics to Membrane Biophysics
(Lisbon, Portugal)
2018 Impact of Ca2+-dependent PI(4,5)P2 clustering on the properties of PI(4,5)P2 binding proteins. 1st Biophysics Party
(Lisbon, Portugal)
2017/02/14 Spatial organization of nuclear structures by dual colour super-resolution microscopy. Biophysical Society 61st Annual Meeting
(New Orleans, United States)
2017 Nanoscale compartmentalization of PI(4,5)P2 in living cells and model membranes, a fluorescence spectroscopy and FRET imaging study. XXV National SPQ Meeting
(Lisbon, Portugal)
2016 Analysis of nanoscale compartmentalization of PI(4,5)P2 in the plasma membrane through FRET imaging. Spanish-Portuguese Meeting for Advanced Optical Microscopy (SPAOM)
(Bilbao, Spain)
2016 Analysis of nanoscale compartmentalization of PI(4,5)P2 in living cells and model membranes through fluorescence spectroscopy and FRET imaging. 5th International Iberian Biophysics Congress
(Porto, Portugal)
2016 Quantification of nanoscale compartmentalization in the plasma membrane of living cells through FRET imaging. II Congresso Regional da Sociedade Brasileira de Biofísica
(Aracaju, Brazil)
2015/02/10 Analysis of PI(4,5)P2 lateral organization at the plasma membrane of living cells through FRET. Biophysical Society 59th Annual Meeting
(Baltimore, United States)
2015 FRET Analysis of the Nanoscale Organization of PI(4,5)P2 in Living Cells. Biophysics of Proteins at Surfaces: Assembly, Activation, Signaling (Biophysical Society Sponsored meeting)
(Madrid, Spain)
2014/06/05 Modulators of PI(4,5)P2 lateral distribution in model systems and cellular membranes. EMBO workshop: Cellular Imaging of Lipids
(Naples, Italy)
2014/05/01 Physiological Ca2+ concentrations induce PI(4,5)P2 clustering and have an impact in PI(4,5)P2 partition properties. 4th Portuguese Young Chemists Meeting
(Coimbra, Portugal)
2014 Detection and characterization of calcium induced PI(4,5)P2 clustering in membranes. Application of advanced fluorescence and microscopy methodologies. 43rd Annual Meeting SBBQ
(Iguaçu, Brazil)
2014 Characterization of PI(4,5)P2 distribution in membranes through fluorescence spectroscopy and FRET microscopy. 1st International Caparica Conference on Chromogenic and Emissive Materials
(Lisboa, Portugal)
2014 Application of advanced fluorescence and microscopy approaches to the study of PI(4,5)P2 clustering in membranes. Xth International Symposium on Cell Surface Macromolecules
(Calcutta, India)
2013/07/15 PI(4,5)P2 acts as a lipid cancium sensor in the presence pf physiological calcium concentrations. 9th European Biophysics Congress
(Lisboa, Portugal)
2013/02/05 Physiological calcium concentrations induce PI(4,5)P2 clustering: PI(4,5)P2 as a lipidic calcium sensor. Biophysical Society 57th Annual Meeting
(Philadelphia, United States)
2013 Size and dynamics of PI(4,5)P2 clusters, induced by calcium concentrations in the physiological range in model systems of membranes: Application of advanced fluorescence and microscopy approaches. VIII Ibero-American Congress of Biophysics
(Valparaíso, Chile)
2013 Physiological concentrations of calcium induce PI(4,5)P2 clustering in membranes: Application of advanced fluorescence and microscopy approaches. Annual Meeting of the Turkish Biophysical Society
(Trabzon, Turkey)
2012 Tethering of lipid membranes reorganizes the distribution of lipid domains. XXXV Spanish Society for Biochemistry and Molecular Biology (SEBBM) Congress
(Seville, Spain)

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2023 - Current Deciphering alterations in mechanical properties of the nucleus during healthy human aging.
Supervisor of Aivaras Vilutis
Universidade NOVA de Lisboa NOVA Medical School, Portugal
2023 - Current From health to disease: nuclear envelope dynamics in ageing
Supervisor of Francisco Rocha
2020/09/01 - 2022/01 Development of membrane models mimicking the inner nuclear membrane in healthy and premature aging
Supervisor of Maria Inês Silva
 Genética Molecular e Biomedicina (Master)
Universidade Nova de Lisboa, Portugal

Event organisation

Event name
Type of event (Role) 		Institution / Organization
2024/04 - 2024/04 "Building bridges: carving new paths for biophysicists in a biology landscape". Networking Event aiming to ignite the creation of long-lasting productive tights between biophysics and biologists within the Greater Lisbon Area. Funds granted by the American Biophysical Society. (2024/04 - 2024/04)
Meeting (Co-organisor)
 Instituto de Medicina Molecular, Portugal
2013 - 2013 Volunteer at the 9th European Biophysics Congress, Lisbon, Portugal. (2013 - 2013)

Event participation

Activity description
Type of event 		Event name
Institution / Organization
2023/12/13 - Current Chair of the oral communications
Other
 Dia da Investigação
Universidade de Lisboa Faculdade de Medicina, Portugal
2023/03/27 - 2023/03/31 Experimental data analysis using business intelligence - 3rd Ed (Power BI)
Workshop
 Experimental data analysis using business intelligence - 3rd Ed
2022/10/17 - 2022/10/21 Introduction to Python and Machine Learning for the Biosciences | 4th Edition
Workshop
 Introduction to Python and Machine Learning for the Biosciences | 4th Edition
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2020/03 - 2020/03 Chair of the session "GUVs" at the Membrane Biophysics Meeting, Drübeck, Germany
Meeting
DGfB Membrane Section, Germany
2019 - 2019 SMALP User Meeting
2018/04 - 2018/04 7th Wroclaw-Prague seminar on biophysics of lipids. Wroclaw, Poland.
Seminar
2018 - 2018 Lisbon Biophysics Networking Meeting: From Protein Dynamics to Membrane Biophysics.
Meeting
2015/04 - 2015/04 585. WE-Heraeus seminar: advanced microscopy of membrane biophysics. Bad Honef, Germany.
Seminar
2014/06 - 2014/06 EMBO workshop: cellular imaging of lipids. Vico Equense, Naples, Italy.
Workshop
2014 - 2014 European Researcher's Night, Lisbon, Portugal.
Exhibition
2012/09 - 2012/09 FEBS course - microspectroscopy: visualization of protein dynamics in living cells. Nijmegen/Wageningen, The Netherlands.
Workshop
2011/11 - 2011/11 10th short course of the portuguese biophysical society - nanosciences for life. Santarém, Portugal.
2010 - 2010 9th short course of the portuguese biophysical society - protein folding, misfolding and disease. Santarém, Portugal.

Jury of academic degree

Topic
Role 		Candidate name (Type of degree)
Institution / Organization
2022 Eletroporação e estabilidade de membranas aniônicas com diferentes níveis de complexidade.
(Thesis) Main arguer
 Fernanda Leomil (PhD)
Universidade Federal de São Paulo, Escola Paulista de Medicina, Brazil
2021/11/19 Characterization of potential biophysical regulators for clustering of the multifunctional lipid PI(4,5)P2.
(Thesis) Main arguer
 Raquel Alexandra Bispo Guerreiro (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2020/12/21 Novel strategies for anti-cancer therapeutics using lipid droplets labelling and cationic polyurea dendrimers
(Thesis) Main arguer
 Patrícia Marta Antunes (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2019/02/28 Sphingosine-induced alterations in membrane biophysical properties: biological relevance in the pathophysiology of human disease
(Thesis) Main arguer
 Ana Cláudia Nunes Carreira (PhD)
Universidade de Lisboa Faculdade de Farmácia, Portugal

Ad Hoc journal article review

Journal title (ISSN) Publisher
2023 - Current Cells
2023 - Current Biomolecules
2022 - Current Biophysical Journal
2022 - Current Chemistry and Physics of Lipids
2022 - Current Membranes
2018 - Current ACS Omega

Committee member

Activity description
Role 		Institution / Organization
2021/01 - Current Young Researchers and Innovators Committee of the EpiLipidNET COST action (former Early Career Investigators Committee)
Member
2020 - 2021 MC Substitute for Portugal in the EpiLipidNET COST action
Member

Conference scientific committee

Conference name Conference host
2024/05 - 2024/05 Biophysics Festival - 4th Meeting of Young Biophysicists Young Portuguese Biophysicists Association

Course / Discipline taught

Academic session Degree Subject (Type) Institution / Organization
2019/01/10 - 2019/01/10 Pharmaceutical Biophysics (single class on fluorescence microscopy) Medicines and pharmaceutical innovation (Doutoramento) Universidade de Lisboa Faculdade de Farmácia, Portugal
2018/11/21 - 2018/11/21 Biochemistry of Neurodegeneration (single class on membrane-associated oligomerization of amyloid peptides) Bioquímica (Mestrado integrado) Universidade de Lisboa Faculdade de Ciências, Portugal
2016 - 2016 Advanced Optical Fluorescence Microscopy Methods II (single class on FRET applications) PhD course Istituto Italiano di Tecnologia, Italy

Journal scientific committee

Journal title (ISSN) Publisher
2023 - Current Frontiers in Molecular Biosciences (Review Editor on the Editorial Board of Molecular Biophysics )

Mentoring / Tutoring

Topic Student name
2019/01 - 2019/10 Characterization of lipid bilayer nanodiscs of different lipid composition. Mariana Ribeiro
Distinctions

Award

2022 SPB Bursary (for participation in The Biochemistry Global Summit (IUBMB-FEBS-PABMB 2022 Congress))
Sociedade Portuguesa de Bioquímica, Portugal
2020 Travel Award
DGfB Membrane Section, Germany
2020 Best Poster Award at the Membrane Biophysics Meeting
DGfB Membrane Section, Germany
2014 Youth Travel Grant
European Molecular Biology Organization, Germany
2013 International Travel Award
Biophysical Society, United States

Other distinction

2014 EBSA Bursary for a working visit
European Biophysical Societies' Association, Germany
2010 Erasmus Program
Uniwersytet Wroclawski Wydzial Biotechnologii, Poland